CSPI02G08610 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G08610 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 6.7e-152
Identity = 322/713 (45.16%), Postives = 469/713 (65.78%), Query Frame = 0

Query: 5   KSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIP 64
           KS +  +   +  P+K   +P+        R    R + +KPKSRF +P+ P+       
Sbjct: 45  KSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPV------- 104

Query: 65  ESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIG------------PENENERKAGRRRR 124
             T +L+  ++++     S S +S ++  +  +G             ++E+E    + + 
Sbjct: 105 -DTSILEEEVREQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKL 164

Query: 125 RKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRL 184
            +  R KI+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G L
Sbjct: 165 NREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGML 224

Query: 185 VSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN-- 244
           V+ W + ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +  
Sbjct: 225 VTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPA 284

Query: 245 -NKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSG 304
             KVL  + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG
Sbjct: 285 ATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSG 344

Query: 305 PPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSL 364
            PL EE   + V R     H+S +            T+ +       + IDM ++ K+  
Sbjct: 345 LPLMEEA--ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKR 404

Query: 365 ERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKN 424
           E + SAW+++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+N
Sbjct: 405 E-KVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRN 464

Query: 425 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 484
           VA+P   YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAH
Sbjct: 465 VAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAH 524

Query: 485 SLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKT 544
           SLNDTKTAV+QL+KL +A+++V+ +VI LL+L VATTKVL   ++QL+ + F+  +TCK 
Sbjct: 525 SLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKN 584

Query: 545 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 604
           +FESI+FVFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPIS
Sbjct: 585 LFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPIS 644

Query: 605 NFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 664
           N+ RSP+M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+
Sbjct: 645 NYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKL 704

Query: 665 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           KM+L   HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 705 KMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CSPI02G08610 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 7.6e-140
Identity = 304/697 (43.62%), Postives = 443/697 (63.56%), Query Frame = 0

Query: 28  TTTPTPTRTKTLRRLRTKPKSRF-DEPNYPLSTPKTIPESTDLLQPPLQQEDSTSSSSSS 87
           T+  +  RTKTL +++T+ +      P YP       P S + L P     ++   + + 
Sbjct: 170 TSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGN-LNPGFSGRNTKPGTPNQ 229

Query: 88  SSSSDYEDGEIGPENENERKAGRRRRRKGKRKKINKRVLIEWILFLTITTCLICALTLES 147
             S D E+ E  P +E +   G R      ++KI   V+IEWI  + I   LIC+L +  
Sbjct: 230 GGSKDLEEEE-DPFSEEDLPEGLR------KEKICVWVIIEWIFLILIIASLICSLVIPY 289

Query: 148 LQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSF 207
           L+ K +W L +WKW ++V+V+ CGRLVS W+V + V+ +E NF+ R++VLYFVYG+RK  
Sbjct: 290 LRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPV 349

Query: 208 QNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFLIAVLIGATIWLLKILLVKVLASS 267
           QNC WLGLVLIAW  +F    +    + VL  V + LI +L+   IWL+K LLVKVLASS
Sbjct: 350 QNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASS 409

Query: 268 FHVATFFDRMKESVFNHYILETLSGPP-----LDEEERDKEV-------NRRRRLVHMSK 327
           FH++T+FDR++ES+F  Y++ETLSGPP     ++EE+   +V        +   L   + 
Sbjct: 410 FHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAV 469

Query: 328 SLPARWREGGG-----------GQTLSR--SKRQDSCQKIDMERLRKLSLERRPSAWSVK 387
           S P +   G G              LSR  SK++   + I ++ L++++  +  SAW +K
Sbjct: 470 SSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMN-TKNVSAWKMK 529

Query: 388 RLVSYVRSSGLSTISRTVDDFANAE----SEITSESEARNCAQRVFKNVAKPGARYIEEE 447
           +L++ ++   LST+   + D    E    ++I SE EA+  A+++F+NVA+PG+RYI  E
Sbjct: 530 KLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYME 589

Query: 448 DLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQL 507
           D +RFL ++E      LFEGA E  KISKS  +NWVV+A+ ER+ALA +LNDTKTAV +L
Sbjct: 590 DFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRL 649

Query: 508 HKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMH 567
           H++   +V ++I++I LL+LG+ATTK L VI+SQLLLV F+F N+CKTIFE++IFVFVMH
Sbjct: 650 HRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMH 709

Query: 568 PFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTV 627
           PFDVGDRC IDGV M VEEMNIL+TVFLRFDN+KI YPNS+L TKPI+N+ RSPDM D +
Sbjct: 710 PFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAI 769

Query: 628 DFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNL 687
           +F + ++T  +  TALR+ +  Y+++K  HW P   +V +++  ++ +K+++   H MN 
Sbjct: 770 EFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNH 829

Query: 688 QNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           QN  ER  RR  L+ E+ R+   L I+Y L P  + V
Sbjct: 830 QNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 857

BLAST of CSPI02G08610 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.9e-138
Identity = 289/702 (41.17%), Postives = 442/702 (62.96%), Query Frame = 0

Query: 19  DKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIPESTDLLQPPLQQED 78
           D E +  S    P    + TL ++RT  +SR  +P     TP+  P++ D+    + +  
Sbjct: 148 DGEVVKCSGNNAPIQRSSSTLLKMRT--RSRLSDP----PTPQLPPQTADMKSGRIPKSG 207

Query: 79  STSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKINKRVLIEWILFLTITTCL 138
              S     S         G E E++  A      + ++ K++  +++EW+  + I    
Sbjct: 208 QMKSGFFGKSPKTQ-----GEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGF 267

Query: 139 ICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYF 198
           +C L + SL++K++W L++WKW  +V+V+ CGRLVS W+V ++VF IERNF+LR+RVLYF
Sbjct: 268 VCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYF 327

Query: 199 VYGLRKSFQNCAWLGLVLIAWMIMFPD---VHHNNKVLLKVFRFLIAVLIGATIWLLKIL 258
           VYG+RK+ QNC WLGLVL+AW  +F +      N K L  V +  + +L+G  +WL+K L
Sbjct: 328 VYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTL 387

Query: 259 LVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVNRRRRL--------- 318
           LVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E ++++E   R  +         
Sbjct: 388 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPG 447

Query: 319 --------------VHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRP 378
                            S  L      GGGG   ++    DS  K++ + +         
Sbjct: 448 GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNV--------- 507

Query: 379 SAWSVKRLVSYVRSSGLSTISRTVDDFA---NAESEITSESEARNCAQRVFKNVAKPGAR 438
           SAW +KRL++ +R+  L+T+   + D +   +  ++I SE EA+  A+++F NVAKPG++
Sbjct: 508 SAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSK 567

Query: 439 YIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKT 498
           +I   D++RFL D+E      LFEGA ET +ISKS+ +NWVV+A+ ER+ALA +LNDTKT
Sbjct: 568 FIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKT 627

Query: 499 AVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIF 558
           AV +LHK+ + VV +II+VI L++LG+ +TK L V++SQ+++V F+F N CK +FESII+
Sbjct: 628 AVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIY 687

Query: 559 VFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPD 618
           +FV+HPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+K+ YPNS+L TK I N+ RSPD
Sbjct: 688 LFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPD 747

Query: 619 MSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQ 678
           M D ++F+I ++T  + I  +++ +  YIE K  HW P   +V K++E+++ +++++   
Sbjct: 748 MGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPT 807

Query: 679 HTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           H MN Q+  E+  RRS L+ E+ ++   L I+Y L P ++ V
Sbjct: 808 HRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of CSPI02G08610 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 4.2e-138
Identity = 295/672 (43.90%), Postives = 431/672 (64.14%), Query Frame = 0

Query: 43  RTKPKSRFDEPNYPLSTPKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENE 102
           R K KSR  +P  P     T P          ++     S     S      G  G E E
Sbjct: 191 RNKTKSRLQDPPTP-----THPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEE 250

Query: 103 NERK--AGRRRRRKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKW 162
            E           + KR K++  V +EWI  + I T L+C+LT+ +LQ K  W L++WKW
Sbjct: 251 EEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKW 310

Query: 163 CLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWM 222
            + V+V+ CGRLVS W+V ++VF++E+NF  R+RVLYFVYG+RKS QNC WLGLVL+AW 
Sbjct: 311 EVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWH 370

Query: 223 IMFP---DVHHNNKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESV 282
            +F    +    +  L  V R L+ +L+   IWL+K +LVKVLASSFH++T+FDR++ES+
Sbjct: 371 FLFDKKVERETRSTALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESL 430

Query: 283 FNHYILETLSGPPLDEEERDKEVNRR-------------RRLVHMSKSLPARWREGGGGQ 342
           F  Y++ETLSGPPL E +R +E  ++              +L    K+    + + G   
Sbjct: 431 FTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSP 490

Query: 343 TLSR--SKRQDSCQKIDMERLRKLSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFANA 402
            L+R  SKR +  + I +++L++++  +  SAW++KRL++ +    +ST+ + + D    
Sbjct: 491 GLNRIGSKRGEDGEGIRIDQLKRMN-TKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQE 550

Query: 403 ESEIT---SESEARNCAQRVFKNVAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETG 462
           + + T   SE EA+  A+++F NV +PG+RYI  ED LRFL +EE      LFEGA E+ 
Sbjct: 551 DEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESD 610

Query: 463 KISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATT 522
           KISKS  +NWVV A+ ER+ALA +LNDTKTAV +LH++ + V+ +III+I LL+LG+ATT
Sbjct: 611 KISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 670

Query: 523 KVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILST 582
           + L V++SQLLLV F+F N+CKTIFE+IIF+FVMHPFDVGDRC IDGV + VEEMNIL+T
Sbjct: 671 RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTT 730

Query: 583 VFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIE 642
           VFLR+DN+KI YPNSVL TKPI+N+ RSPDM D V+F + ++T  + ITA+++ +  Y++
Sbjct: 731 VFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVD 790

Query: 643 SKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLG 692
           +K  +W P   +V   +++++ +K+++ + H MN Q+  ER  RR  L+ E+ +    L 
Sbjct: 791 NKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELD 850

BLAST of CSPI02G08610 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 6.1e-129
Identity = 299/712 (41.99%), Postives = 450/712 (63.20%), Query Frame = 0

Query: 13  LTIDPPDKEQISPS---PTTTPTP---TRTKTL-RRLRTKPKSRFDEP---NYPLSTP-- 72
           ++I  P+  + S S   P   P+P    R K+L R + +KPKSRF E     Y  +    
Sbjct: 52  ISIPTPEIYKFSGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREEN 111

Query: 73  --KTIPE----------STDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAG 132
             +++ E          S D   P  +   S +S++ S  + +  D       ENE    
Sbjct: 112 GGRSLREQFGAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPD-------ENEEIYK 171

Query: 133 RRRRRKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVF 192
           + +  + KR  +     +E ++F+ I   LI +LT++ + +  IW LE WKWC++VMV  
Sbjct: 172 KVKLHRVKRSGMKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTL 231

Query: 193 CGRLVSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFP-DVH 252
            G LV+ W +  +VF+IE+N++LR++VLYFV+GL+K+ Q   W  LVLIAW+ +F  DV 
Sbjct: 232 SGMLVTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVK 291

Query: 253 HNNKV---LLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILE 312
              K    L  +   ++++L+G+ ++L+K   +KVLAS F+V  FF+R++ESVF+ Y+L+
Sbjct: 292 RTRKTKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQ 351

Query: 313 TLSGPPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLR 372
           TLSGPPL EE   + V R     H+S +   R ++G         K +D  + IDM ++ 
Sbjct: 352 TLSGPPLIEEA--ENVGRVPSTGHLSFT---RTKDG---------KVKDK-KVIDMGKVH 411

Query: 373 KLSLERRPSAWSVKRLVSYVRSSGLSTISRTVDDFAN----AESEITSESEARNCAQRVF 432
           ++  E + SAW+++ L+  V +SG+STIS T+D+  N     + EIT+E EA   A  VF
Sbjct: 412 RMKQE-KVSAWTMRVLIEAVGTSGISTISSTLDEVNNKKERTDKEITNEMEAVAAAYDVF 471

Query: 433 KNVAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKAL 492
            NVAKP   YIEE+DLLRF+  EEV+ + PL E A +TGKI++  F  WVV+ Y  RK +
Sbjct: 472 NNVAKPNHNYIEEDDLLRFMIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTI 531

Query: 493 AHSLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTC 552
            HSLNDTKTAV+QL KL + ++ VI  ++ +++L +A+TK+L V +SQ L + FM  +TC
Sbjct: 532 GHSLNDTKTAVKQLDKLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTC 591

Query: 553 KTIFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKP 612
           K IFES +FVFVMHP+DVGDRCV+DGV + VEE+++L+TVFL+ DNEK++YPNSVL++KP
Sbjct: 592 KNIFESFMFVFVMHPYDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKP 651

Query: 613 ISNFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVD 672
           ISNF RSPDM D VDF I  ST  + I  L+  +  Y+ +  +HW P+  ++V+ IEN++
Sbjct: 652 ISNFYRSPDMGDYVDFGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMN 711

Query: 673 KMKMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVT 693
           K+ +++ VQHT+N Q + E++ RR+ LI+ +KR+ E+L I Y LLPQ+V +T
Sbjct: 712 KLVLNILVQHTINFQVYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNLT 739

BLAST of CSPI02G08610 vs. ExPASy TrEMBL
Match: A0A0A0LHK4 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_2G118330 PE=3 SV=1)

HSP 1 Score: 1337.8 bits (3461), Expect = 0.0e+00
Identity = 705/706 (99.86%), Postives = 705/706 (99.86%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP 60
           MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP
Sbjct: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI 120
           KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI
Sbjct: 61  KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA 480
           KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA
Sbjct: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA 480

Query: 481 VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 706

BLAST of CSPI02G08610 vs. ExPASy TrEMBL
Match: A0A5D3B9B7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00100 PE=3 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 684/707 (96.75%), Postives = 693/707 (98.02%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+ RKSDNDHLVLTIDPP KEQISPSPTTT T TRTKTLRRL  +KPKSRFDEPNYPLST
Sbjct: 1   MEPRKSDNDHLVLTIDPPQKEQISPSPTTT-TITRTKTLRRLNFSKPKSRFDEPNYPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQPP + EDSTSSSSSSSSSS+YEDGEIG ENENERKAGRRRRRKGKRKK
Sbjct: 61  PRTIPESTDLLQPPPEHEDSTSSSSSSSSSSEYEDGEIGLENENERKAGRRRRRKGKRKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTLES+QEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLESIQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWL LVLIAWMIMFPDVHH NKVLLKVFRF
Sbjct: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLALVLIAWMIMFPDVHHKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE
Sbjct: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
           VNR R L+HMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR
Sbjct: 301 VNRGRGLMHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF
Sbjct: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFENLGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 706

BLAST of CSPI02G08610 vs. ExPASy TrEMBL
Match: A0A1S3C4Z6 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103496913 PE=3 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 684/707 (96.75%), Postives = 693/707 (98.02%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+ RKSDNDHLVLTIDPP KEQISPSPTTT T TRTKTLRRL  +KPKSRFDEPNYPLST
Sbjct: 1   MEPRKSDNDHLVLTIDPPQKEQISPSPTTT-TITRTKTLRRLNFSKPKSRFDEPNYPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQPP + EDSTSSSSSSSSSS+YEDGEIG ENENERKAGRRRRRKGKRKK
Sbjct: 61  PRTIPESTDLLQPPPEHEDSTSSSSSSSSSSEYEDGEIGLENENERKAGRRRRRKGKRKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTLES+QEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLESIQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWL LVLIAWMIMFPDVHH NKVLLKVFRF
Sbjct: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLALVLIAWMIMFPDVHHKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE
Sbjct: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
           VNR R L+HMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR
Sbjct: 301 VNRGRGLMHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF
Sbjct: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFENLGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 706

BLAST of CSPI02G08610 vs. ExPASy TrEMBL
Match: A0A6J1HL20 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111464526 PE=3 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 632/707 (89.39%), Postives = 659/707 (93.21%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+VRK +NDHLVL IDPP+KEQIS S      P RTKTLRRL  +KPKSRFDEPN+PLST
Sbjct: 1   MEVRKPENDHLVLAIDPPEKEQISLS------PARTKTLRRLNFSKPKSRFDEPNFPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQP  +++   SSSSSS S  + ED EIG  NE     GR+R+RK K+KK
Sbjct: 61  PRTIPESTDLLQPTAEED---SSSSSSYSEEEDEDEEIGAGNE-----GRKRKRKKKKKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTLESLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLESLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           +LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRF
Sbjct: 181 LLVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE
Sbjct: 241 LIAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEAEMDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
            NR+RRL  MSKSLPAR +E GGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KR
Sbjct: 301 ENRKRRLQKMSKSLPARLQESGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           L+SYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRF
Sbjct: 361 LMSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFV+TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVVTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 693

BLAST of CSPI02G08610 vs. ExPASy TrEMBL
Match: A0A6J1KX29 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111498994 PE=3 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 620/708 (87.57%), Postives = 659/708 (93.08%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+VRK + DHLVLTIDPP+KEQISPS      PTRTKT RRL  +KPKSRF+EP++  S 
Sbjct: 1   MEVRKPEKDHLVLTIDPPEKEQISPS------PTRTKTFRRLNFSKPKSRFEEPDFSRSI 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQPP+ +EDS+SSSSS SSSS+YED EIGPENE+  K GRRR+RK KRKK
Sbjct: 61  PRTIPESTDLLQPPV-EEDSSSSSSSYSSSSEYEDEEIGPENEDGGKGGRRRKRKAKRKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKR LIEWILFLTITTCLICALTL+SLQEKQIWSL+VWKWCLIVMV+FCGRL SEWLVG
Sbjct: 121 INKRALIEWILFLTITTCLICALTLKSLQEKQIWSLQVWKWCLIVMVIFCGRLGSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           +LVF+IERNFMLRERVLYFVYGLR SFQNC WLGLVLIAWMIMFPDVHHNNK LLKVFRF
Sbjct: 181 LLVFIIERNFMLRERVLYFVYGLRNSFQNCTWLGLVLIAWMIMFPDVHHNNKALLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKE+VFNHYILETLSGPPLDEEERD  
Sbjct: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKENVFNHYILETLSGPPLDEEERDNV 300

Query: 301 VNRRRRLVHMSKSLPARWREGG-GGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVK 360
           +N +RRL+  SKS+P RWREGG GGQ LSRSKRQ SC+KID+ERLRKLSL+R+PSAWSVK
Sbjct: 301 INCQRRLLQQSKSMPVRWREGGEGGQNLSRSKRQGSCKKIDIERLRKLSLQRKPSAWSVK 360

Query: 361 RLVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLR 420
           RLVSYVRSSGLSTISRTVDDFANAESEITSESEAR CAQRVFKNVA PGARYIEEEDLLR
Sbjct: 361 RLVSYVRSSGLSTISRTVDDFANAESEITSESEARRCAQRVFKNVAVPGARYIEEEDLLR 420

Query: 421 FLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLA 480
           FLK EEVNTIFPLFEGAIETGKISKSAFRNWV+HAYIERKALAHSLNDTKTAV QLHKLA
Sbjct: 421 FLKVEEVNTIFPLFEGAIETGKISKSAFRNWVLHAYIERKALAHSLNDTKTAVHQLHKLA 480

Query: 481 SAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDV 540
           SAVV VIIIVISLLVLGVAT KVL V+TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDV
Sbjct: 481 SAVVSVIIIVISLLVLGVATIKVLLVVTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDV 540

Query: 541 GDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTI 600
           GDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTV+FTI
Sbjct: 541 GDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVNFTI 600

Query: 601 DVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFP 660
           D STSFDNITAL+KAMQIYIESKPKHWSPKH+LVVKEIEN+DKMKMSLCVQHTMN QNF 
Sbjct: 601 DASTSFDNITALKKAMQIYIESKPKHWSPKHTLVVKEIENMDKMKMSLCVQHTMNHQNFT 660

Query: 661 ERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           ERN+RRSDLILELKRVFENLGIKYHLLPQ+VL+T FNLTN +  +PSS
Sbjct: 661 ERNSRRSDLILELKRVFENLGIKYHLLPQQVLLTHFNLTNAQTLLPSS 701

BLAST of CSPI02G08610 vs. NCBI nr
Match: XP_011649063.1 (mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN61410.1 hypothetical protein Csa_006738 [Cucumis sativus])

HSP 1 Score: 1337.8 bits (3461), Expect = 0.0e+00
Identity = 705/706 (99.86%), Postives = 705/706 (99.86%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP 60
           MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP
Sbjct: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTP 60

Query: 61  KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI 120
           KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI
Sbjct: 61  KTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKI 120

Query: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180
           NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV
Sbjct: 121 NKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGV 180

Query: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240
           LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL
Sbjct: 181 LVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRFL 240

Query: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300
           IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV
Sbjct: 241 IAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEV 300

Query: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360
           NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL
Sbjct: 301 NRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKRL 360

Query: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420
           VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL
Sbjct: 361 VSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRFL 420

Query: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA 480
           KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA
Sbjct: 421 KDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLASA 480

Query: 481 VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540
           VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD
Sbjct: 481 VVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVGD 540

Query: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600
           RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV
Sbjct: 541 RCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTIDV 600

Query: 601 STSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660
           STSFD ITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER
Sbjct: 601 STSFDIITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPER 660

Query: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS
Sbjct: 661 NNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 706

BLAST of CSPI02G08610 vs. NCBI nr
Match: XP_008457175.1 (PREDICTED: mechanosensitive ion channel protein 10 [Cucumis melo] >KAA0039640.1 mechanosensitive ion channel protein 10 [Cucumis melo var. makuwa] >TYJ95524.1 mechanosensitive ion channel protein 10 [Cucumis melo var. makuwa])

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 684/707 (96.75%), Postives = 693/707 (98.02%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+ RKSDNDHLVLTIDPP KEQISPSPTTT T TRTKTLRRL  +KPKSRFDEPNYPLST
Sbjct: 1   MEPRKSDNDHLVLTIDPPQKEQISPSPTTT-TITRTKTLRRLNFSKPKSRFDEPNYPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQPP + EDSTSSSSSSSSSS+YEDGEIG ENENERKAGRRRRRKGKRKK
Sbjct: 61  PRTIPESTDLLQPPPEHEDSTSSSSSSSSSSEYEDGEIGLENENERKAGRRRRRKGKRKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTLES+QEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLESIQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWL LVLIAWMIMFPDVHH NKVLLKVFRF
Sbjct: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLALVLIAWMIMFPDVHHKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE
Sbjct: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
           VNR R L+HMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR
Sbjct: 301 VNRGRGLMHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF
Sbjct: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFENLGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 706

BLAST of CSPI02G08610 vs. NCBI nr
Match: XP_038876133.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 664/707 (93.92%), Postives = 684/707 (96.75%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+VRKSDNDHLVLT+DPP+KEQISPSPT+      TKTLRRL  +KPKSRFDEPN+P ST
Sbjct: 1   MEVRKSDNDHLVLTVDPPEKEQISPSPTS------TKTLRRLNFSKPKSRFDEPNFPHST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQPPL +EDSTSSSSSSSSSS+YED EIGPENENE K+GRRR+RKGKRKK
Sbjct: 61  PRTIPESTDLLQPPL-EEDSTSSSSSSSSSSEYEDEEIGPENENEGKSGRRRKRKGKRKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLIC+LTLESLQEKQIWSLEVWKWCLIVMV+FCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICSLTLESLQEKQIWSLEVWKWCLIVMVIFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH  NKVLLKVFRF
Sbjct: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHQKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVLIGAT+WLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE
Sbjct: 241 LIAVLIGATVWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
           +NRRRRL+ MSKSLPARW EGGGGQ LSRSKRQ S +KIDMERLRKLSLERRPSAWSVKR
Sbjct: 301 MNRRRRLLQMSKSLPARWLEGGGGQILSRSKRQGSSKKIDMERLRKLSLERRPSAWSVKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           LVSYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF
Sbjct: 361 LVSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKIEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVL VITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLVVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHW+PKHSLVVKEIENVDKMKMSLCVQHTMN QNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWNPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFEILGIKYHLLPQEVVVTQFNLTNGRMAIPSS 700

BLAST of CSPI02G08610 vs. NCBI nr
Match: XP_022964525.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >KAG6593772.1 Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 632/707 (89.39%), Postives = 659/707 (93.21%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+VRK +NDHLVL IDPP+KEQIS S      P RTKTLRRL  +KPKSRFDEPN+PLST
Sbjct: 1   MEVRKPENDHLVLAIDPPEKEQISLS------PARTKTLRRLNFSKPKSRFDEPNFPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPESTDLLQP  +++   SSSSSS S  + ED EIG  NE     GR+R+RK K+KK
Sbjct: 61  PRTIPESTDLLQPTAEED---SSSSSSYSEEEDEDEEIGAGNE-----GRKRKRKKKKKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTLESLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLESLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           +LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRF
Sbjct: 181 LLVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE
Sbjct: 241 LIAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEAEMDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
            NR+RRL  MSKSLPAR +E GGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KR
Sbjct: 301 ENRKRRLQKMSKSLPARLQESGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           L+SYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRF
Sbjct: 361 LMSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFV+TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVVTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 693

BLAST of CSPI02G08610 vs. NCBI nr
Match: XP_023513519.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 630/707 (89.11%), Postives = 659/707 (93.21%), Query Frame = 0

Query: 1   MDVRKSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLR-TKPKSRFDEPNYPLST 60
           M+VRK +NDHLVL IDPP+KEQIS S      P RTKTLRRL  +KPKSRFDEPN+PLST
Sbjct: 1   MEVRKPENDHLVLAIDPPEKEQISLS------PARTKTLRRLNFSKPKSRFDEPNFPLST 60

Query: 61  PKTIPESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKK 120
           P+TIPEST+LLQP  +++   SSSSSS S  + ED EIG  NE     GR+R+RK K+KK
Sbjct: 61  PRTIPESTELLQPTAEED---SSSSSSYSEEEDEDEEIGAGNE-----GRKRKRKKKKKK 120

Query: 121 INKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180
           INKRVLIEWILFLTITTCLICALTL+SLQ KQIWSLEVWKWCLIVMVVFCGRLVSEWLVG
Sbjct: 121 INKRVLIEWILFLTITTCLICALTLKSLQAKQIWSLEVWKWCLIVMVVFCGRLVSEWLVG 180

Query: 181 VLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHHNNKVLLKVFRF 240
           +LVF+IERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVH+ NKVLLKVFRF
Sbjct: 181 LLVFIIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMFPDVHNKNKVLLKVFRF 240

Query: 241 LIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKE 300
           LIAVL+GATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDE E DKE
Sbjct: 241 LIAVLVGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEAEMDKE 300

Query: 301 VNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRPSAWSVKR 360
            NR+RRL  MSKSLPAR +E GGGQ LSRSKRQDSC+KIDME LRKLSL+RRPSAWS KR
Sbjct: 301 ENRKRRLQKMSKSLPARLQESGGGQILSRSKRQDSCKKIDMEMLRKLSLQRRPSAWSAKR 360

Query: 361 LVSYVRSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARYIEEEDLLRF 420
           L+SYV+SSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGAR I E+DLLRF
Sbjct: 361 LMSYVKSSGLSTISRTVDDFANAESEITSESEARNCAQRVFKNVAKPGARSIGEDDLLRF 420

Query: 421 LKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480
           LK EEV TIFPLFEGA+ETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS
Sbjct: 421 LKVEEVTTIFPLFEGAMETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQLHKLAS 480

Query: 481 AVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540
           AVVIVIIIVISLLVLGVATTKVLFV+TSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG
Sbjct: 481 AVVIVIIIVISLLVLGVATTKVLFVVTSQLLLVGFMFQNTCKTIFESIIFVFVMHPFDVG 540

Query: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600
           DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID
Sbjct: 541 DRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTVDFTID 600

Query: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNLQNFPE 660
           VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMN QNFPE
Sbjct: 601 VSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNHQNFPE 660

Query: 661 RNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFNLTNGRMAIPSS 707
           RNNRRSDLILELKRVFE LGIKYHLLPQEV+VTQFNLTNGRMAIPSS
Sbjct: 661 RNNRRSDLILELKRVFELLGIKYHLLPQEVVVTQFNLTNGRMAIPSS 693

BLAST of CSPI02G08610 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 539.3 bits (1388), Expect = 4.7e-153
Identity = 322/713 (45.16%), Postives = 469/713 (65.78%), Query Frame = 0

Query: 5   KSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIP 64
           KS +  +   +  P+K   +P+        R    R + +KPKSRF +P+ P+       
Sbjct: 45  KSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPV------- 104

Query: 65  ESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIG------------PENENERKAGRRRR 124
             T +L+  ++++     S S +S ++  +  +G             ++E+E    + + 
Sbjct: 105 -DTSILEEEVREQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKL 164

Query: 125 RKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRL 184
            +  R KI+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G L
Sbjct: 165 NREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGML 224

Query: 185 VSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN-- 244
           V+ W + ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +  
Sbjct: 225 VTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPA 284

Query: 245 -NKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSG 304
             KVL  + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG
Sbjct: 285 ATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSG 344

Query: 305 PPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSL 364
            PL EE   + V R     H+S +            T+ +       + IDM ++ K+  
Sbjct: 345 LPLMEEA--ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKR 404

Query: 365 ERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKN 424
           E + SAW+++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+N
Sbjct: 405 E-KVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRN 464

Query: 425 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 484
           VA+P   YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAH
Sbjct: 465 VAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAH 524

Query: 485 SLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKT 544
           SLNDTKTAV+QL+KL +A+++V+ +VI LL+L VATTKVL   ++QL+ + F+  +TCK 
Sbjct: 525 SLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKN 584

Query: 545 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 604
           +FESI+FVFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPIS
Sbjct: 585 LFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPIS 644

Query: 605 NFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 664
           N+ RSP+M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+
Sbjct: 645 NYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKL 704

Query: 665 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           KM+L   HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 705 KMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CSPI02G08610 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 539.3 bits (1388), Expect = 4.7e-153
Identity = 322/713 (45.16%), Postives = 469/713 (65.78%), Query Frame = 0

Query: 5   KSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIP 64
           KS +  +   +  P+K   +P+        R    R + +KPKSRF +P+ P+       
Sbjct: 45  KSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPV------- 104

Query: 65  ESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIG------------PENENERKAGRRRR 124
             T +L+  ++++     S S +S ++  +  +G             ++E+E    + + 
Sbjct: 105 -DTSILEEEVREQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKL 164

Query: 125 RKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRL 184
            +  R KI+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G L
Sbjct: 165 NREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGML 224

Query: 185 VSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN-- 244
           V+ W + ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +  
Sbjct: 225 VTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPA 284

Query: 245 -NKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSG 304
             KVL  + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG
Sbjct: 285 ATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSG 344

Query: 305 PPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSL 364
            PL EE   + V R     H+S +            T+ +       + IDM ++ K+  
Sbjct: 345 LPLMEEA--ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKR 404

Query: 365 ERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKN 424
           E + SAW+++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+N
Sbjct: 405 E-KVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRN 464

Query: 425 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 484
           VA+P   YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAH
Sbjct: 465 VAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAH 524

Query: 485 SLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKT 544
           SLNDTKTAV+QL+KL +A+++V+ +VI LL+L VATTKVL   ++QL+ + F+  +TCK 
Sbjct: 525 SLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKN 584

Query: 545 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 604
           +FESI+FVFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPIS
Sbjct: 585 LFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPIS 644

Query: 605 NFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 664
           N+ RSP+M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+
Sbjct: 645 NYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKL 704

Query: 665 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           KM+L   HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 705 KMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CSPI02G08610 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 539.3 bits (1388), Expect = 4.7e-153
Identity = 322/713 (45.16%), Postives = 469/713 (65.78%), Query Frame = 0

Query: 5   KSDNDHLVLTIDPPDKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIP 64
           KS +  +   +  P+K   +P+        R    R + +KPKSRF +P+ P+       
Sbjct: 45  KSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPV------- 104

Query: 65  ESTDLLQPPLQQEDSTSSSSSSSSSSDYEDGEIG------------PENENERKAGRRRR 124
             T +L+  ++++     S S +S ++  +  +G             ++E+E    + + 
Sbjct: 105 -DTSILEEEVREQLGAGFSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKL 164

Query: 125 RKGKRKKINKRVLIEWILFLTITTCLICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRL 184
            +  R KI+   LIE   F+ I + L+ +LT+  L+    W LEVWKWC++VMV+F G L
Sbjct: 165 NREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGML 224

Query: 185 VSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSFQNCAWLGLVLIAWMIMF-PDVHHN-- 244
           V+ W + ++VF+IE NF+LR +VLYFV+GL+KS Q   WL L+L+AW+++F  DV  +  
Sbjct: 225 VTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPA 284

Query: 245 -NKVLLKVFRFLIAVLIGATIWLLKILLVKVLASSFHVATFFDRMKESVFNHYILETLSG 304
             KVL  + R LI++L GA  WL+K LL+K+LA++F+V  FFDR+++SVF+ Y+L+TLSG
Sbjct: 285 ATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSG 344

Query: 305 PPLDEEERDKEVNRRRRLVHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSL 364
            PL EE   + V R     H+S +            T+ +       + IDM ++ K+  
Sbjct: 345 LPLMEEA--ERVGREPSTGHLSFA------------TVVKKGTVKEKKVIDMGKVHKMKR 404

Query: 365 ERRPSAWSVKRLVSYVRSSGLSTISRTVDDFA------NAESEITSESEARNCAQRVFKN 424
           E + SAW+++ L+  VR+SGLSTIS T+D+ A       A+ EITSE EA   A  VF+N
Sbjct: 405 E-KVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRN 464

Query: 425 VAKPGARYIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAH 484
           VA+P   YIEEEDLLRF+  EEV+ +FPLF+GA ETG+I++ AF  WVV  Y  R+ALAH
Sbjct: 465 VAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAH 524

Query: 485 SLNDTKTAVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKT 544
           SLNDTKTAV+QL+KL +A+++V+ +VI LL+L VATTKVL   ++QL+ + F+  +TCK 
Sbjct: 525 SLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKN 584

Query: 545 IFESIIFVFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPIS 604
           +FESI+FVFVMHP+DVGDRCV+DGV M VEEMN+L+TVFL+ +NEK+YYPN+VL TKPIS
Sbjct: 585 LFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPIS 644

Query: 605 NFRRSPDMSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKM 664
           N+ RSP+M +TV+F+I  ST    I  L++ +  Y+E  P+HW+P HS+VVKEIEN++K+
Sbjct: 645 NYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKL 704

Query: 665 KMSLCVQHTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLVTQFN 696
           KM+L   HT+  Q   ERN RR++L L +KR+ E+L I Y LLPQ++ +T+ N
Sbjct: 705 KMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINLTKKN 734

BLAST of CSPI02G08610 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 499.2 bits (1284), Expect = 5.4e-141
Identity = 304/697 (43.62%), Postives = 443/697 (63.56%), Query Frame = 0

Query: 28  TTTPTPTRTKTLRRLRTKPKSRF-DEPNYPLSTPKTIPESTDLLQPPLQQEDSTSSSSSS 87
           T+  +  RTKTL +++T+ +      P YP       P S + L P     ++   + + 
Sbjct: 170 TSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGN-LNPGFSGRNTKPGTPNQ 229

Query: 88  SSSSDYEDGEIGPENENERKAGRRRRRKGKRKKINKRVLIEWILFLTITTCLICALTLES 147
             S D E+ E  P +E +   G R      ++KI   V+IEWI  + I   LIC+L +  
Sbjct: 230 GGSKDLEEEE-DPFSEEDLPEGLR------KEKICVWVIIEWIFLILIIASLICSLVIPY 289

Query: 148 LQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYFVYGLRKSF 207
           L+ K +W L +WKW ++V+V+ CGRLVS W+V + V+ +E NF+ R++VLYFVYG+RK  
Sbjct: 290 LRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPV 349

Query: 208 QNCAWLGLVLIAWMIMFP---DVHHNNKVLLKVFRFLIAVLIGATIWLLKILLVKVLASS 267
           QNC WLGLVLIAW  +F    +    + VL  V + LI +L+   IWL+K LLVKVLASS
Sbjct: 350 QNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASS 409

Query: 268 FHVATFFDRMKESVFNHYILETLSGPP-----LDEEERDKEV-------NRRRRLVHMSK 327
           FH++T+FDR++ES+F  Y++ETLSGPP     ++EE+   +V        +   L   + 
Sbjct: 410 FHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAV 469

Query: 328 SLPARWREGGG-----------GQTLSR--SKRQDSCQKIDMERLRKLSLERRPSAWSVK 387
           S P +   G G              LSR  SK++   + I ++ L++++  +  SAW +K
Sbjct: 470 SSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMN-TKNVSAWKMK 529

Query: 388 RLVSYVRSSGLSTISRTVDDFANAE----SEITSESEARNCAQRVFKNVAKPGARYIEEE 447
           +L++ ++   LST+   + D    E    ++I SE EA+  A+++F+NVA+PG+RYI  E
Sbjct: 530 KLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYME 589

Query: 448 DLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKTAVQQL 507
           D +RFL ++E      LFEGA E  KISKS  +NWVV+A+ ER+ALA +LNDTKTAV +L
Sbjct: 590 DFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRL 649

Query: 508 HKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIFVFVMH 567
           H++   +V ++I++I LL+LG+ATTK L VI+SQLLLV F+F N+CKTIFE++IFVFVMH
Sbjct: 650 HRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMH 709

Query: 568 PFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPDMSDTV 627
           PFDVGDRC IDGV M VEEMNIL+TVFLRFDN+KI YPNS+L TKPI+N+ RSPDM D +
Sbjct: 710 PFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAI 769

Query: 628 DFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQHTMNL 687
           +F + ++T  +  TALR+ +  Y+++K  HW P   +V +++  ++ +K+++   H MN 
Sbjct: 770 EFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNH 829

Query: 688 QNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           QN  ER  RR  L+ E+ R+   L I+Y L P  + V
Sbjct: 830 QNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINV 857

BLAST of CSPI02G08610 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 494.6 bits (1272), Expect = 1.3e-139
Identity = 289/702 (41.17%), Postives = 442/702 (62.96%), Query Frame = 0

Query: 19  DKEQISPSPTTTPTPTRTKTLRRLRTKPKSRFDEPNYPLSTPKTIPESTDLLQPPLQQED 78
           D E +  S    P    + TL ++RT  +SR  +P     TP+  P++ D+    + +  
Sbjct: 148 DGEVVKCSGNNAPIQRSSSTLLKMRT--RSRLSDP----PTPQLPPQTADMKSGRIPKSG 207

Query: 79  STSSSSSSSSSSDYEDGEIGPENENERKAGRRRRRKGKRKKINKRVLIEWILFLTITTCL 138
              S     S         G E E++  A      + ++ K++  +++EW+  + I    
Sbjct: 208 QMKSGFFGKSPKTQ-----GEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGF 267

Query: 139 ICALTLESLQEKQIWSLEVWKWCLIVMVVFCGRLVSEWLVGVLVFVIERNFMLRERVLYF 198
           +C L + SL++K++W L++WKW  +V+V+ CGRLVS W+V ++VF IERNF+LR+RVLYF
Sbjct: 268 VCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYF 327

Query: 199 VYGLRKSFQNCAWLGLVLIAWMIMFPD---VHHNNKVLLKVFRFLIAVLIGATIWLLKIL 258
           VYG+RK+ QNC WLGLVL+AW  +F +      N K L  V +  + +L+G  +WL+K L
Sbjct: 328 VYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTL 387

Query: 259 LVKVLASSFHVATFFDRMKESVFNHYILETLSGPPLDEEERDKEVNRRRRL--------- 318
           LVKVLASSFH++T+FDR++ES+F  Y++ETLSGPPL E ++++E   R  +         
Sbjct: 388 LVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPG 447

Query: 319 --------------VHMSKSLPARWREGGGGQTLSRSKRQDSCQKIDMERLRKLSLERRP 378
                            S  L      GGGG   ++    DS  K++ + +         
Sbjct: 448 GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNV--------- 507

Query: 379 SAWSVKRLVSYVRSSGLSTISRTVDDFA---NAESEITSESEARNCAQRVFKNVAKPGAR 438
           SAW +KRL++ +R+  L+T+   + D +   +  ++I SE EA+  A+++F NVAKPG++
Sbjct: 508 SAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSK 567

Query: 439 YIEEEDLLRFLKDEEVNTIFPLFEGAIETGKISKSAFRNWVVHAYIERKALAHSLNDTKT 498
           +I   D++RFL D+E      LFEGA ET +ISKS+ +NWVV+A+ ER+ALA +LNDTKT
Sbjct: 568 FIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKT 627

Query: 499 AVQQLHKLASAVVIVIIIVISLLVLGVATTKVLFVITSQLLLVGFMFQNTCKTIFESIIF 558
           AV +LHK+ + VV +II+VI L++LG+ +TK L V++SQ+++V F+F N CK +FESII+
Sbjct: 628 AVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIY 687

Query: 559 VFVMHPFDVGDRCVIDGVHMFVEEMNILSTVFLRFDNEKIYYPNSVLLTKPISNFRRSPD 618
           +FV+HPFDVGDRC IDGV M VEEMNIL+TVFLRFDN+K+ YPNS+L TK I N+ RSPD
Sbjct: 688 LFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPD 747

Query: 619 MSDTVDFTIDVSTSFDNITALRKAMQIYIESKPKHWSPKHSLVVKEIENVDKMKMSLCVQ 678
           M D ++F+I ++T  + I  +++ +  YIE K  HW P   +V K++E+++ +++++   
Sbjct: 748 MGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPT 807

Query: 679 HTMNLQNFPERNNRRSDLILELKRVFENLGIKYHLLPQEVLV 692
           H MN Q+  E+  RRS L+ E+ ++   L I+Y L P ++ V
Sbjct: 808 HRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINV 829