Cucsat.G10803 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10803
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionBeta-amylase
Locationctg1681: 140469 .. 143565 (-)
RNA-Seq ExpressionCucsat.G10803
SyntenyCucsat.G10803
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTGGGAGAAGTGTAGACACTGTAGACGACAATGGTCAAAGATGAACTGTGAAATTGATACACTAAATAAAGAAAAGATAAAAAAAGAGAATCGCTGTTGATTCCTCTGCTTTTGTGCGAAATTTCAGTAAAATGGAACTGAAAATCAATCAAAAAGCAGCAAACACCTGAAAATAGGATCGACACATCGAATCTAACACAACTCACATTTCACCCTCATAATCTACAAACCCATCTAAGAATTCAAGACAGATTTTTTTTCTTTTCTGAAAGATATAAATGAGCTAAATCAATGAAAAAATGGATCTCAGAACTTCTTGTTGGTCTTACGAATCGAGGACTGCAGCTTAGCATGCATTGGTCCCGGACTCACCAGTGTCGTACTGGGCGGAGTTAACGGCGAAGCAGCTTGGGTTTCTGGAGGAGATGGAGATTGAGATGCCGGCGGCGGCGGCGGTAAGGGATTTTGGATGGTATCAGGTGGAGGTGGGGAAATCGGAATAGTGTCAGGCGGAGGTGGAGAAGTTGGAATAACGTCAGGTGGAGGTGGGGAAGCCGGAGTAGCGTCAGGTGGAGGTGGGGAAGCTGAAGTAGCGTCAGACGGAGGCGGAAAAGCAGGAATCGAATTTTGGGGTGGTGGGTTTGGATTAGAATACGATGGGTTGGGTGGGTTCTGTGGCTGATTGGAGGCGGAGATGGGGGAGGCGCCGGGAGTGGCGGAATTGGGGTTGGAAAAGACGGCCGGAGGAGAAAAACAGGAGTTGCCGGAGCACTTGTGGGATTTTTGGAGGCCGTGGCGCATAAATCCGAAGCCAAGAACTAGACCAAGAACCACCAAAACAGTTACAATGAAGATGAAGATCTTAGTTGCTTTACCCACGTAACACATTTTTTATTTCCAGACTTCCAAATTTCAGATTCCAAAAAATAGACACAGTTCTTCGCTTCACAAGAAAAAGATTTGGGCTTTGTTTCTCTTTCGTCTTTTTCTCATGGCGGTTGGAGAAGACGAGCAAGATGAGAGAGATAGAGAGTAGGGAACTGGACTGGGCTACTCACTGAGTTGTAACTTGAAATGGGATCGGATCCACGTGAAGACCAATAAAACCGTCACCATTCTTTTACCCTTTTTACTTTTTTTGCTGAGTGAATGACAGGTAAGATTTTCACTTTTTACTGTCCTTACATCGATCAAGAGAGTGATAGTGGGTGAACGGAAGGTTGCTAAGGTTAAAAAAAGATTTAACTCTCTCTCTTAACTAGTGATAATAGCTCTTAAGCATGGTTTTTATGTAACCAAAGTGAAGTGAACTGGCTTAGATAAATAATAATAGCTCAAAGATTCAAATCTTCAAATTCCACATGACTGAAAAAGAAAGAGAAAAAATCTAAATTTTTACCTCAAGGGATTGGTGGGCAATGGCGGTCAGCAATACACCATGCCCATGGGTTGAAGTGGGGCACTAATGACATATGGTAAAAGCCTGTAGGATTGGACTTCGAGAAAAATCCGTTCACTTTTGAGGTTAAAAAATAAAAATGAAAAGGGATTTTGTTTGTTATCACATACCTGTTGAATCGACATCAAGAATCGAGCCAACGAAGCATGTCTGCCAAGTCATCCCTCTTTGAGATAGGGGTTAACCAACTGTGGCTCTGAGAAATAGCTGAACTATGCATCACATTTCCCCTCTTTAAAAGAAGCCTACGCAGATTGAATGTTTGTAAATAATGTTGAAAACTAAACCATCATAATTTTAATTTGTCAATATTTTGAATTCTTCCAAACTGTCACTATTCACAAAAACCTTTCTGACTCGTGCAAGTCTCAGCAAGAATACAACCAATAAAGCATCAAATGACCCATCTTCCTCATCTTTTACCAATCATGGACTTGGACATGAAGAAACTACAAGATATCTTCTTTCTTGAATTAGAGAAGAACGTGTATGGATTGTGTTTCACTAGCTCATTCGTTATCCTTACCTTATGTGTGAATTCATGTTCGCTCACTTCCTATCTGCTCTAATGCTCATAAACAATAGAAGGAAATGGCTTCAGACACAATCATGGCAAGTTTTGTGTGCCAAGTGCATAGTGGAATAATAAAGAGAATAGCACATGTTCAAGGTGAAGTTAAATGTAAATTGCAAGTAAAGAATCTAAAACCCAACTGGTCTCTCCCATGTTGGTCTATAGCACCAAGAATCAATCTTCGGACTCCAGCATATGAGACGACTACGATTGAACAAGAGGTAACTATGTTCAGGGATGTACATCTTTCTTCTATCAGATATCCTGAACCAGTGGCATAGATGGCCTTTCAAAAGCTTGAAGAGGCCATCAACCTTGATTTAATCTTTACGTAGTATGCAGAAACATCAAGGATCAACAAATGCCTTGTTTTTCTTTTCTTTTTTTTTGGGGGGGGAAGCCTATATTCTCTCTCATCAACTCGATACTTTATTTATATATTCTAGTTTGGGACTCATGTCATTTAATTGCTTTATGCACTTTTATCCACTTTGCAGTCTTCCATGAAAATTTTCTTTCATGAAGGTATCAACAAGCAGCAGTCCTGACTACGATAAAAAGTTGATACAAAACTATGTGCCAGTTTATGTGATGCTCCCAGTAAGTTGCATACAGAATCTTGTATACTCGCGTTCATCAGAGTCAAACAAGTTCAGTTTATCATTCTTCTTATTTTTTTAACACCAGTTGGGAGTTGTTACAACTGATAACGAACTGGAAGATAGAGATGGGATAAAGAAACAACTCAAACAACTACAAGCAGCAGACGTGGATGGGGTAATGATTGATGTTTGGTGGGGTATTGTAGAATCCAAAGGGCCAAATCAGTATGATTGGACTGCATATAGGAGCTTATTTAAGATAATTCAAGAATGTGGCTTGAAACTTCAAGCTATTATGTCATTCCACCAATGTGGAGGGAATGTGGGAGATTCTGTTAACATTCCACTCCCCAGTTGGATTCTCAAAATTGGAGAATTAAATCCTGATATCTTCTACACGAACCGATCAGGGACCAGGAACAAGGAGTACCTCACTCTTGGAGTTGATAACCAACCTCTCTTTCATGGGCGAACTGCTGTAGAGGTCAGCTCAGCTCCTTGAAACTGCCTGACAGATAAACGAATTCAAACAAATAATCAGTCGCCAAAAATTTCAAATCAGTCACAGTAAATCAATTACTCCTCACACTGAAATGTTTCCATTGATGGGTACCCTTTGATCCCTGATGTAAATGTTCAGAAGAAAATTCTGAAAATGGAAGAACATTAAAATTAGATTACAAATGTTGATCCTTTATATTGCACCTCATTCCTTATAATAATTTCAAATTACGGAACTACACAGTTATACAGGGATTACATGAAGAGTTTCAGAGAAAATATGGTGGATTTTTTAGACAATGGTCTTATAATAGACATTGAAGTGGGACTCGGCCCCGCCGGGGAGTTGAGGTATCCTTCATATGTACAAAATCAGGGATGGGAATTTCCTGGTATTGGAGAATTCCAGGTAAGGCAAGTTTTAGAACATTTTAAAGGACACCCTGTTCTGGTTTTAACTTCATTTTTCAAATATTACAGTGCTATGACAAATATCTCAAAATGGAATTCAAAGGGGCTGCAGTAACAGCGGGACACCCTGAATGGAAGCTTCCAGATAATGCTGGAACATACAATGACGCCCCTGAATCTACAGAGTTCTTCAGATCAAATGGCACTTATCAATCTGACGAAGGAAGGTTTTTCTTGACATGGTATTCCAACAAGTTATTGAACCATGGTGATCAGATCCTTGAAGAAGCAAACCAAGTTTTCCTTGGTTGTAAACTTAAATTAGCAGCCAAAGTATGTCCCCAAGAAAATATATTTCTACATCTTTATAATTATAGAAACATCTAAAAGCATAAGTTATTGTCTTTCTTCTGAAGTCATGTCAGTAAAAGTTATTGTACTTCCTCATAAAAACAGGTAGCAGGGATCCACTGGTGGTATCAAACAGAAAACCATGCAGCTGAGCTTACATCAGGATACTATAATTTGAAAACTAGAGATGGATACCGACCTATAGCACGGATGCTATCAAGACACCATGCAATTTTGAATTTTACATGTCTAGAGATGAGAAATTATGAGCATATTTCAAAAGCCAAAAGTGGACCTGAGGAACTGGTTCAGCAGGTAAAGTAGAACCAAATTCATCGTGTGTGTGTTATTAGGCAGATTAAAATCACGAATTCTAGGAATGATTGAATTTGTGACCAAGTATTAATCATCACAAACAGGTTTTAAGTGGGGGCTGGAGAGAAGGCATTCCAGTAGCAGGAGAGAATGCACTACCAAGGTATGACAATGCAGCTTACAATCAAATTCTTTTGAATGCTAGACCAACCGGAATCAACAAAGAGGGTCAACCAAAGCATAAGATGTTTGGAGTGACATACCTTCGACTTTGTAACAAACTCCTGCAGAAAAGAAATTTCAATATATTCAAGAGCTTTGTGATGAAGATGCATGCTGATCAGGTAATGAATTACATTATTTACGAAAAGCGAGGACTGAATAAAGGTTAATCAAATTCCTCCTTTCTGCCTTTGCACTCAGCTGGCTTGTCTTTTGTGTTCTATAGGGATACTGCCCCAATCCAGAGGACTATAACTGCTATGTTGTCCCACTCAATCAGTCAAAAGAAAAAATTTCAATGGAAGCACTTTTAGAAGCAACTGAACCATTGGAGCCATTCTCATGGGATAAGGAAACCGATACTCCAATTGGTGGTCCTTTTGTAGATTTCTTTAATAGAATACTATACATATTTAAGTGAAATAAGAGATACACCAAACGTTACTATAGAATCCATGTTTAAGAAACAATGTTCCTGAGTTCCTCCTGCTATTGTATAAAATCGATATGAAATGAAGTTAAATAATTACCGAAAATTACCACAGGAACAAATGCATC

Coding sequence (CDS)

ATGAAGGTATCAACAAGCAGCAGTCCTGACTACGATAAAAAGTTGATACAAAACTATGTGCCAGTTTATGTGATGCTCCCATTGGGAGTTGTTACAACTGATAACGAACTGGAAGATAGAGATGGGATAAAGAAACAACTCAAACAACTACAAGCAGCAGACGTGGATGGGGTAATGATTGATGTTTGGTGGGGTATTGTAGAATCCAAAGGGCCAAATCAGTATGATTGGACTGCATATAGGAGCTTATTTAAGATAATTCAAGAATGTGGCTTGAAACTTCAAGCTATTATGTCATTCCACCAATGTGGAGGGAATGTGGGAGATTCTGTTAACATTCCACTCCCCAGTTGGATTCTCAAAATTGGAGAATTAAATCCTGATATCTTCTACACGAACCGATCAGGGACCAGGAACAAGGAGTACCTCACTCTTGGAGTTGATAACCAACCTCTCTTTCATGGGCGAACTGCTGTAGAGTTATACAGGGATTACATGAAGAGTTTCAGAGAAAATATGGTGGATTTTTTAGACAATGGTCTTATAATAGACATTGAAGTGGGACTCGGCCCCGCCGGGGAGTTGAGGTATCCTTCATATGTACAAAATCAGGGATGGGAATTTCCTGGTATTGGAGAATTCCAGTGCTATGACAAATATCTCAAAATGGAATTCAAAGGGGCTGCAGTAACAGCGGGACACCCTGAATGGAAGCTTCCAGATAATGCTGGAACATACAATGACGCCCCTGAATCTACAGAGTTCTTCAGATCAAATGGCACTTATCAATCTGACGAAGGAAGGTTTTTCTTGACATGGTATTCCAACAAGTTATTGAACCATGGTGATCAGATCCTTGAAGAAGCAAACCAAGTTTTCCTTGGTTGTAAACTTAAATTAGCAGCCAAAGTAGCAGGGATCCACTGGTGGTATCAAACAGAAAACCATGCAGCTGAGCTTACATCAGGATACTATAATTTGAAAACTAGAGATGGATACCGACCTATAGCACGGATGCTATCAAGACACCATGCAATTTTGAATTTTACATGTCTAGAGATGAGAAATTATGAGCATATTTCAAAAGCCAAAAGTGGACCTGAGGAACTGGTTCAGCAGGTTTTAAGTGGGGGCTGGAGAGAAGGCATTCCAGTAGCAGGAGAGAATGCACTACCAAGGTATGACAATGCAGCTTACAATCAAATTCTTTTGAATGCTAGACCAACCGGAATCAACAAAGAGGGTCAACCAAAGCATAAGATGTTTGGAGTGACATACCTTCGACTTTGTAACAAACTCCTGCAGAAAAGAAATTTCAATATATTCAAGAGCTTTGTGATGAAGATGCATGCTGATCAGGGATACTGCCCCAATCCAGAGGACTATAACTGCTATGTTGTCCCACTCAATCAGTCAAAAGAAAAAATTTCAATGGAAGCACTTTTAGAAGCAACTGAACCATTGGAGCCATTCTCATGGGATAAGGAAACCGATACTCCAATTGGTGGTCCTTTTGTAGATTTCTTTAATAGAATACTATACATATTTAAGTGA

Protein sequence

MKVSTSSSPDYDKKLIQNYVPVYVMLPLGVVTTDNELEDRDGIKKQLKQLQAADVDGVMIDVWWGIVESKGPNQYDWTAYRSLFKIIQECGLKLQAIMSFHQCGGNVGDSVNIPLPSWILKIGELNPDIFYTNRSGTRNKEYLTLGVDNQPLFHGRTAVELYRDYMKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEFKGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQILEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHAILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLNARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCYVVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK
Homology
BLAST of Cucsat.G10803 vs. ExPASy Swiss-Prot
Match: P10538 (Beta-amylase OS=Glycine max OX=3847 GN=BMY1 PE=1 SV=3)

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-154
Identity = 254/338 (75.15%), Postives = 283/338 (83.73%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFL++GLIIDIEVGLGPAGELRYPSY Q+QGWEFP IGEFQCYDKYLK +F
Sbjct: 159 MKSFRENMSDFLESGLIIDIEVGLGPAGELRYPSYPQSQGWEFPRIGEFQCYDKYLKADF 218

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           K A   AGHPEW+LPD+AG YND PEST FF+SNGTY +++G+FFLTWYSNKLLNHGDQI
Sbjct: 219 KAAVARAGHPEWELPDDAGKYNDVPESTGFFKSNGTYVTEKGKFFLTWYSNKLLNHGDQI 278

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           L+EAN+ FLGCK+KLA KV+GIHWWY+ ENHAAELT+GYYNL  RDGYRPIARMLSRHHA
Sbjct: 279 LDEANKAFLGCKVKLAIKVSGIHWWYKVENHAAELTAGYYNLNDRDGYRPIARMLSRHHA 338

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMR+ E  S AKSGP+ELVQQVLSGGWRE I VAGENALPRYD  AYNQI+LN
Sbjct: 339 ILNFTCLEMRDSEQPSDAKSGPQELVQQVLSGGWREDIRVAGENALPRYDATAYNQIILN 398

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           A+P G+N  G PK  MFGVTYLRL + LLQK NFNIFK FV+KMHADQ YC NP+ YN  
Sbjct: 399 AKPQGVNNNGPPKLSMFGVTYLRLSDDLLQKSNFNIFKKFVLKMHADQDYCANPQKYNHA 458

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGG 339
           + PL  S  KI +E LLEAT+P  PF W  ETD  + G
Sbjct: 459 ITPLKPSAPKIPIEVLLEATKPTLPFPWLPETDMKVDG 496

BLAST of Cucsat.G10803 vs. ExPASy Swiss-Prot
Match: O64407 (Beta-amylase OS=Vigna unguiculata OX=3917 GN=BMY1 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 2.5e-152
Identity = 244/338 (72.19%), Postives = 289/338 (85.50%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFL + +IIDIEVGLGPAGELRYPSY QNQGW FPGIGEFQCYDKYLK EF
Sbjct: 159 MKSFRENMSDFLKSEVIIDIEVGLGPAGELRYPSYPQNQGWVFPGIGEFQCYDKYLKAEF 218

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           K AA  AGH EW+LPD+AGTYND PESTEFF++NGTY +++G+FFLTWYSN+LLNHGD+I
Sbjct: 219 KAAAARAGHSEWELPDDAGTYNDVPESTEFFKTNGTYLTEKGKFFLTWYSNQLLNHGDEI 278

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           L+EAN+ FLGCK+ LA KV+GIHWWY+ +NHAAELT+GYYNL  RDGYRPIA+M+SRHHA
Sbjct: 279 LDEANKAFLGCKVNLAIKVSGIHWWYKAQNHAAELTAGYYNLDDRDGYRPIAKMVSRHHA 338

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
            LNFTCLEMR+ E  S A+SGP+ELVQQVLSGGWRE I VAGENAL RYD  AYNQI+LN
Sbjct: 339 SLNFTCLEMRDSEQSSDAQSGPQELVQQVLSGGWRENIEVAGENALSRYDATAYNQIILN 398

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP G+NK+G PKH+M+GVTYLRL ++LLQ+ NF+IFK FV+KMHADQ YC +P++YN  
Sbjct: 399 ARPQGVNKDGPPKHRMYGVTYLRLSDELLQQSNFDIFKKFVVKMHADQDYCEDPQEYNHG 458

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGG 339
           + PL +S+ KI ++ L EAT+P+ PF WD ETD  + G
Sbjct: 459 IPPLKRSEPKIPVDVLNEATKPIPPFPWDSETDMKVDG 496

BLAST of Cucsat.G10803 vs. ExPASy Swiss-Prot
Match: O65015 (Beta-amylase OS=Trifolium repens OX=3899 GN=BMY1 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.2e-149
Identity = 242/338 (71.60%), Postives = 285/338 (84.32%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM +FL + LIIDIEVGLGPAGELRYPSY QNQGW FPGIGEFQCYDKYLK +F
Sbjct: 159 MKSFRENMSEFLKSELIIDIEVGLGPAGELRYPSYPQNQGWVFPGIGEFQCYDKYLKADF 218

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           K AA  AGH EW+LPD+AGTYND PESTEFF++NGTY +++G+FFLTWYSN+LLNHGDQI
Sbjct: 219 KAAAAKAGHSEWELPDDAGTYNDIPESTEFFKTNGTYLTEKGKFFLTWYSNQLLNHGDQI 278

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           L+EAN+ FLGCK+KLA KV+GIHWWY+ +NHAAELT+GYYNL  RDGYRPIA+M+SRHH 
Sbjct: 279 LDEANKAFLGCKVKLAIKVSGIHWWYKAQNHAAELTAGYYNLDDRDGYRPIAKMVSRHHG 338

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMR+ E  S A+S P+ELVQQVLSGGWRE I VAGENAL RYD  AYNQI+LN
Sbjct: 339 ILNFTCLEMRDSEQSSDAQSAPQELVQQVLSGGWRENIEVAGENALSRYDATAYNQIILN 398

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP G+NK+G PK +M+GVTYLRL + LLQ+ NF IFK FV+KMHADQ +C +P++YN  
Sbjct: 399 ARPQGVNKDGPPKLRMYGVTYLRLSDDLLQESNFEIFKKFVVKMHADQSHCDDPQEYNHA 458

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGG 339
           + PL +S   I ++ LLEAT+P+ PF WD ETD  + G
Sbjct: 459 IPPLKRSGPNIPVDDLLEATKPILPFPWDSETDMKVDG 496

BLAST of Cucsat.G10803 vs. ExPASy Swiss-Prot
Match: O22585 (Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 6.5e-148
Identity = 240/338 (71.01%), Postives = 282/338 (83.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM D L + +IIDIEVGLGPAGELRYPSY QNQGW+FPGIGEFQCYDKYL+  F
Sbjct: 159 MKSFRENMSDLLKSEVIIDIEVGLGPAGELRYPSYPQNQGWQFPGIGEFQCYDKYLRESF 218

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           K AA  AGH EW+LPD+AGTYND PESTEFF++NGTY +++G+FFLTWYSN+LLNHGDQI
Sbjct: 219 KAAAAKAGHSEWELPDDAGTYNDVPESTEFFKTNGTYLTEKGKFFLTWYSNQLLNHGDQI 278

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           L+EAN+ FLGCK+KLA KV+GIHWWY+  NHAAELT+GYYNL  RDGYRPIA+++SRHHA
Sbjct: 279 LDEANKAFLGCKVKLAIKVSGIHWWYKAPNHAAELTAGYYNLDDRDGYRPIAKIVSRHHA 338

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMR+ E  S A S P++LVQQVLSGGWRE I VAGENAL RYD  AYNQI+LN
Sbjct: 339 ILNFTCLEMRDSEQSSDAHSSPQKLVQQVLSGGWRENIEVAGENALSRYDATAYNQIILN 398

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP G+NK+G PK +M+GVTYLRL + L+Q+ NF+IFK FV+KMHADQ YC +PE YN  
Sbjct: 399 ARPQGVNKDGPPKLRMYGVTYLRLSDDLMQQSNFDIFKKFVVKMHADQDYCSDPEKYNHG 458

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGG 339
           + PL +S  KI  + L EAT+P+ PF WD ETD  + G
Sbjct: 459 IPPLKRSGPKIPDDVLNEATKPIPPFPWDSETDMKVDG 496

BLAST of Cucsat.G10803 vs. ExPASy Swiss-Prot
Match: Q8L762 (Beta-amylase 6 OS=Arabidopsis thaliana OX=3702 GN=BAM6 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.5e-139
Identity = 229/337 (67.95%), Postives = 273/337 (81.01%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DF+ +G+IIDIEVGLGPAGELRYPSY + QGW FPGIGEFQCYDKYL+ ++
Sbjct: 224 MKSFRENMEDFISSGVIIDIEVGLGPAGELRYPSYSETQGWVFPGIGEFQCYDKYLRSDY 283

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFR-SNGTYQSDEGRFFLTWYSNKLLNHGDQ 120
           +      GHPEWKLP+NAG YN  P  TEFF  SNGTY  +EG FFL+WYS KLL HGDQ
Sbjct: 284 EEEVRRIGHPEWKLPENAGEYNSVPGETEFFEYSNGTYLKEEGNFFLSWYSKKLLLHGDQ 343

Query: 121 ILEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHH 180
           IL+EAN+VFLGCKLK+AAKV+GIHWWY+TE+HAAELT+GYYNLK RDGYR IA+++ RHH
Sbjct: 344 ILDEANKVFLGCKLKIAAKVSGIHWWYKTESHAAELTAGYYNLKNRDGYRAIAKIMRRHH 403

Query: 181 AILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILL 240
           AILNFTCLEM+N E  +KAKSGP+ELVQQVLS GWREGI VAGENALPR+D   YNQI+L
Sbjct: 404 AILNFTCLEMKNTEQPAKAKSGPQELVQQVLSSGWREGIEVAGENALPRFDRNGYNQIIL 463

Query: 241 NARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNC 300
           NARP G+N++G+P  +MFG TYLRL +KLL + NF+ FK F+ +MHA+Q YC  PE YN 
Sbjct: 464 NARPNGVNQDGKP--RMFGFTYLRLSDKLLNEPNFSTFKMFLKRMHANQEYCSEPERYNH 523

Query: 301 YVVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPI 337
            ++PL +S+   S+E  +E TEP +PF W  ETD  I
Sbjct: 524 ELLPLERSRNDESLEMFMEETEPFDPFPWLDETDMSI 558

BLAST of Cucsat.G10803 vs. NCBI nr
Match: XP_011658713.1 (beta-amylase isoform X2 [Cucumis sativus])

HSP 1 Score: 747 bits (1928), Expect = 4.91e-270
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 166 MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 225

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI
Sbjct: 226 KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 285

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 286 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 345

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN
Sbjct: 346 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 405

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 406 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 465

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK
Sbjct: 466 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 517

BLAST of Cucsat.G10803 vs. NCBI nr
Match: XP_011658712.1 (beta-amylase isoform X1 [Cucumis sativus] >KAE8645749.1 hypothetical protein Csa_020589 [Cucumis sativus])

HSP 1 Score: 747 bits (1928), Expect = 5.46e-269
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 232 MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 291

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI
Sbjct: 292 KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 351

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 352 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 411

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN
Sbjct: 412 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 471

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 472 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 531

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK
Sbjct: 532 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 583

BLAST of Cucsat.G10803 vs. NCBI nr
Match: XP_008455399.1 (PREDICTED: beta-amylase-like isoform X3 [Cucumis melo])

HSP 1 Score: 734 bits (1895), Expect = 2.16e-265
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 142 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 201

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 202 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 261

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 262 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 321

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 322 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 381

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 382 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 441

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 442 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 493

BLAST of Cucsat.G10803 vs. NCBI nr
Match: XP_008455398.1 (PREDICTED: beta-amylase-like isoform X2 [Cucumis melo])

HSP 1 Score: 734 bits (1895), Expect = 5.21e-265
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 166 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 225

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 226 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 285

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 286 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 345

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 346 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 405

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 406 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 465

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 466 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 517

BLAST of Cucsat.G10803 vs. NCBI nr
Match: XP_008455397.1 (PREDICTED: beta-amylase-like isoform X1 [Cucumis melo])

HSP 1 Score: 734 bits (1895), Expect = 5.75e-264
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 232 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 291

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 292 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 351

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 352 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 411

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 412 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 471

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 472 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 531

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 532 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 583

BLAST of Cucsat.G10803 vs. ExPASy TrEMBL
Match: A0A0A0K276 (Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_7G044880 PE=3 SV=1)

HSP 1 Score: 747 bits (1928), Expect = 2.38e-270
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 166 MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 225

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI
Sbjct: 226 KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 285

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 286 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 345

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN
Sbjct: 346 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 405

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 406 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 465

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK
Sbjct: 466 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 517

BLAST of Cucsat.G10803 vs. ExPASy TrEMBL
Match: A0A1S3C0Z1 (Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 1.04e-265
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 142 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 201

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 202 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 261

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 262 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 321

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 322 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 381

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 382 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 441

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 442 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 493

BLAST of Cucsat.G10803 vs. ExPASy TrEMBL
Match: A0A1S3C0E0 (Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 2.52e-265
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 166 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 225

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 226 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 285

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 286 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 345

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 346 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 405

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 406 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 465

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 466 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 517

BLAST of Cucsat.G10803 vs. ExPASy TrEMBL
Match: A0A1S3C222 (Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 2.79e-264
Identity = 344/352 (97.73%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENM DFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF
Sbjct: 232 MKSFRENMEDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 291

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 292 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 351

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 352 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 411

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 412 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 471

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 472 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 531

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 532 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 583

BLAST of Cucsat.G10803 vs. ExPASy TrEMBL
Match: A0A5A7TFD9 (Beta-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003990 PE=3 SV=1)

HSP 1 Score: 734 bits (1894), Expect = 3.95e-264
Identity = 343/352 (97.44%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMEF 60
           MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKM F
Sbjct: 232 MKSFRENMVDFLDNGLIIDIEVGLGPAGELRYPSYVQNQGWEFPGIGEFQCYDKYLKMAF 291

Query: 61  KGAAVTAGHPEWKLPDNAGTYNDAPESTEFFRSNGTYQSDEGRFFLTWYSNKLLNHGDQI 120
           KGAAVTAGHPEW+LPDNAGTYNDAPESTEFFRSNGTYQSDEG+FFLTWYSNKLLNHGDQI
Sbjct: 292 KGAAVTAGHPEWELPDNAGTYNDAPESTEFFRSNGTYQSDEGKFFLTWYSNKLLNHGDQI 351

Query: 121 LEEANQVFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 180
           LEEANQ+FLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA
Sbjct: 352 LEEANQIFLGCKLKLAAKVAGIHWWYQTENHAAELTSGYYNLKTRDGYRPIARMLSRHHA 411

Query: 181 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDNAAYNQILLN 240
           ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYD+AAYNQILLN
Sbjct: 412 ILNFTCLEMRNYEHISKAKSGPEELVQQVLSGGWREGIPVAGENALPRYDHAAYNQILLN 471

Query: 241 ARPTGINKEGQPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 300
           ARP GINKEG+PKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY
Sbjct: 472 ARPNGINKEGEPKHKMFGVTYLRLCNKLLQKRNFNIFKSFVMKMHADQGYCPNPEDYNCY 531

Query: 301 VVPLNQSKEKISMEALLEATEPLEPFSWDKETDTPIGGPFVDFFNRILYIFK 352
           VVPLNQSKEKISMEALLEATEPLEPFSW++ETDTPIGGPFVDFFNRILYIFK
Sbjct: 532 VVPLNQSKEKISMEALLEATEPLEPFSWERETDTPIGGPFVDFFNRILYIFK 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P105381.6e-15475.15Beta-amylase OS=Glycine max OX=3847 GN=BMY1 PE=1 SV=3[more]
O644072.5e-15272.19Beta-amylase OS=Vigna unguiculata OX=3917 GN=BMY1 PE=2 SV=1[more]
O650151.2e-14971.60Beta-amylase OS=Trifolium repens OX=3899 GN=BMY1 PE=2 SV=1[more]
O225856.5e-14871.01Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1[more]
Q8L7622.5e-13967.95Beta-amylase 6 OS=Arabidopsis thaliana OX=3702 GN=BAM6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011658713.14.91e-270100.00beta-amylase isoform X2 [Cucumis sativus][more]
XP_011658712.15.46e-269100.00beta-amylase isoform X1 [Cucumis sativus] >KAE8645749.1 hypothetical protein Csa... [more]
XP_008455399.12.16e-26597.73PREDICTED: beta-amylase-like isoform X3 [Cucumis melo][more]
XP_008455398.15.21e-26597.73PREDICTED: beta-amylase-like isoform X2 [Cucumis melo][more]
XP_008455397.15.75e-26497.73PREDICTED: beta-amylase-like isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0K2762.38e-270100.00Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_7G044880 PE=3 SV=1[more]
A0A1S3C0Z11.04e-26597.73Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1[more]
A0A1S3C0E02.52e-26597.73Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1[more]
A0A1S3C2222.79e-26497.73Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103495571 PE=3 SV=1[more]
A0A5A7TFD93.95e-26497.44Beta-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003990 P... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001554Glycoside hydrolase, family 14PRINTSPR00750BETAAMYLASEcoord: 399..422
score: 65.42
coord: 328..347
score: 61.75
coord: 141..159
score: 55.26
coord: 364..380
score: 54.71
coord: 256..278
score: 76.52
coord: 120..134
score: 73.0
coord: 436..458
score: 70.65
coord: 381..392
score: 79.58
coord: 163..184
score: 82.27
IPR001554Glycoside hydrolase, family 14PFAMPF01373Glyco_hydro_14coord: 89..506
e-value: 2.7E-120
score: 402.0
IPR001554Glycoside hydrolase, family 14PANTHERPTHR31352FAMILY NOT NAMEDcoord: 64..568
IPR001371Glycoside hydrolase, family 14B, plantPRINTSPR00842GLHYDLASE14Bcoord: 444..453
score: 73.08
coord: 506..520
score: 61.03
coord: 402..412
score: 78.32
coord: 246..255
score: 82.31
coord: 458..467
score: 66.15
coord: 473..489
score: 61.54
coord: 491..505
score: 60.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 77..569
e-value: 2.0E-233
score: 777.3
NoneNo IPR availablePANTHERPTHR31352:SF40BETA-AMYLASE 6coord: 64..568
IPR018238Glycoside hydrolase, family 14, conserved sitePROSITEPS00506BETA_AMYLASE_1coord: 167..175
IPR018238Glycoside hydrolase, family 14, conserved sitePROSITEPS00679BETA_AMYLASE_2coord: 256..266
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 82..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10803.T1Cucsat.G10803.T1mRNA
Cucsat.G10803.T12Cucsat.G10803.T12mRNA
Cucsat.G10803.T10Cucsat.G10803.T10mRNA
Cucsat.G10803.T16Cucsat.G10803.T16mRNA
Cucsat.G10803.T2Cucsat.G10803.T2mRNA
Cucsat.G10803.T3Cucsat.G10803.T3mRNA
Cucsat.G10803.T4Cucsat.G10803.T4mRNA
Cucsat.G10803.T5Cucsat.G10803.T5mRNA
Cucsat.G10803.T6Cucsat.G10803.T6mRNA
Cucsat.G10803.T7Cucsat.G10803.T7mRNA
Cucsat.G10803.T8Cucsat.G10803.T8mRNA
Cucsat.G10803.T9Cucsat.G10803.T9mRNA
Cucsat.G10803.T11Cucsat.G10803.T11mRNA
Cucsat.G10803.T13Cucsat.G10803.T13mRNA
Cucsat.G10803.T15Cucsat.G10803.T15mRNA
Cucsat.G10803.T14Cucsat.G10803.T14mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000272 polysaccharide catabolic process
molecular_function GO:0102229 amylopectin maltohydrolase activity
molecular_function GO:0016161 beta-amylase activity