Cucsat.G18215.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G18215.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg3345: 2957542 .. 2962025 (-)
RNA-Seq Expression	Cucsat.G18215.T2
Synteny	Cucsat.G18215.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAAAGTCCATTGTTTCATTGATGGATTAATTAATTCTTGCAATATTCACCACCCATTGACTACCAATTTGCAGATGTACTAATCTTTATTGTTTTCGTTTATTTTCTGATGCAGAAGGACTTAGATTCTTTGATGACATAGCTTTAAGAAAATGGAAATGAAGATGGGAGACAAGGGAAAACTCTAACATGTGAGTGATTCAGAAGAAGTTTATTTAAATCCAGTGTAGAAGTAAGATGATCTTTGTCTGTTTACTAAAAGAAAATTGTGGGGGTTTGAAAGATTTTAACGAGAAAATGGCAAAAAAAAAAAAAACGTAGTTAAGAAAAAGATGAATATTTTAATCTCTGCTGGTTTTTGTTGAAAATTTGAAAATAACCCATTTACGTGTGTTGAACAAGACTGTTGCTTTTTGGGGTCGTGAGAGAAAAGATGGAAAGAAAATAAGAGGGTCTGTAGATCTAATTAAACCATCAAAAGATACCAACATTCTACCATACTCTCTCAACCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTTGGGCGACCAGTTTTGGTGTGATTTCTTCAATCTTATAAATTCATCAAAACCAATGATTGATTGCAATTAGCTTCAACTCCGAGTATTATTAGTATTCAATCCTTCACGAACAATCGTTGGCGTACATTTGTGGTAGAAATAGGGATCTGGGTATACCATAACATCGTCTGATTTTGCCGACATTCTTGTTCTTTGTGGTTTATTGTTGTAAAATCGCATCTGGGTACGAGTGGTTCTTTGATTTTCTGTGATAGATTGCTATAAACATTGAGTTTTTTTTTTTTTTACAATCATTTCGATTTGCGTTTTGGGTTTTCAATCTCCCTGTTTGTAGTGATGGCGGGGAATGGGTTGTTTTACCCGATTCTTGGATTCGCATCCTGTTTGGCTTTCATTTACTTGTCGTTTGGAGATTTGAGGATTGGGGACACTAAAGTAATCCCCATAGTAGATTTTGTAGAGAGGAACGGAACGCAGTTCATGTTGAACGGGAAGGCGTTTTATATTAATGGATGGAATTCTTATTGGTTTATGGACCATTCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCCATGCTGCAGGCGGCGAGAAAGATGGGGCTTACTGTTTGCCGGACTTGGGCTTTCAATGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGAAGGTTTAACGAGCGAGTCTTTAAGGTATTGATTGAGCTGCATTTTCTGAGATTTCTGCTGTTTCTGTGATTGGATTCTTCATTTATTTGATTCTACTCTTAATGCTTTGGTTTTGTGTTCGTAATTTGTTTCTTTTTCTGAAATTTTATGGCAATGTCTGTTTTAGAGAGAGATGAGTGATTCTGCAGGGTTTGAAAGGTTCCATTGGCCAAAGAATGCAAAGCTTGGATGCTTCCACCGACAGAAAAACTGTGTCGGTAGTCATAAAACAATATGGAATTATTTTGTGAATCGCATGATTTTCTTGGAAAAGAAACGGTAGTTCTTGCCATTATGGTTAAGATGAATGAATTAGACTGAAGACAATAAATTATCTGTCAGTTCCTTTTGGCATTTAAAGGTGGATTAATTGAGTATGATTTAAATTCTCATGTTCTGTAACCCCAATAAGAAGCATTATAACTTGTATTTCTGTGGAAGATCAATCTGGTTTTGTTTGTTATCTTGTCAATATAGTTTCAAGTTCAAAAAATGAAGACTGGAATTCATGGAAGAACCAGAAAACTTTCTATTTCCATCTATCTCGCTTTCTCTTTTTGAGAAATATAAGGATAAATGACACATTATCCAAAAAATACACAAGAAAGCATATGTTTGTATATATTTTTTCCATAGCAATTGTTTAGTTTGATGGCGTTTTTTATTTGCGCAAATGCATATGTGAAGTTGTGCTGTTATCAGCTTACGTTTCAATTGGTCCTTCCTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTTCTTAGCTTAGTTAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTTTATGATCCATCAATACGCATTTATTTTAAAAATTATCTCAAGGTACATTCCTTATTTATCAATGAGTTTGTCCAATATGTTTCATAACATTGATGATTTGGTATGCATCCTGAGCTATGCCATGGATTCAAATATATTGATCCAATTGCTATTTACCATTGCATAAGCCATTAGTTCTCGGGTTCAAACAAAAATTCTTACCCTTGTCTTTGCAGATCTTACATTGCTAAATTCTACCTTATGGTGTTTTAGTGGTTCTAATGTGCACTATATTTTTCACTTTGTCAGACCGTCCTGACAAGAAAGAATTCTATCACTGGAATTGAATATAGAAATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCAGGCGATACACTTCAAGTAAGTTTTAAGATTTTGGTTCTTCTGATTTCTGAATGCTGAACCAAATGAGAATTGAAATAAATGGATTCTGGTGTACTCCCCAATTAAGTTGTAAGATTTTGATTCTTCTTGCTTCTAAATGTTAAACCAAAGGAAAACTAACCCTCATTGAACTTACTGTACTCCCGTTTGAGTCTTTTGATAGCTCACTTTTGTAATTTGGGATTGTTACGCAACTTTCTTTCTTCCAAACTTTTTCTTGTGAAAGTTATGAGCTCTCTACTTGGTATTTCTCAACCTCAACTTTGCTTCAAGTTGATTTCTCTAAAGCAAAGTTGCGTTTATTTTTCACCATGTTTTAAGTGTATTTTACCAGAATTAACTCAACGTCTAAAACGAACCTACCAGTCCCAATGTGGAATATATCTTTATCAGTTAATGGCAGAAGCAATATTCACAAATGGACTCATTTTGACCTTGTGTTTGATCCTAATATATGTTTCTCAAGATTTTTGTAAGTATGACCCAATCCTATAGACACTAATGTAGAATGACCCAATCCTAAATAATCAATGAAAACTAACCTTCAGTTGACGTCACATAGCACACTATCGCATTAGAGACATCCCTCCATTCGCAAAATAGTCGGTGCCCTAATGCAATTCATTTCAATAGCAATGTTTCTTTTTAATCAACAGTTGTCCAATCAGCAATGATCTAAAGAAACATCATATTGAAATGAATATCGGTGACTATTTTGCAATGGGTAAGACGTCTTGTTGCAAAAAGAAGTTTCGAGCAGTAGAGTGCTTTGTGATGTTAGTTGAAGGTTCGTTTTCATTGGTTTTTAGGATAGAGTCATTTCACATCAATGCTGCCACCAGGATTGGGTCATCAAATCTCTTGTTTCTCTTCCCACCCCCCACCCCCAAGGGAAGAAAAGAAAAAAGCATCGTTTCTATATCTATGCATAAGCTTATTTTGAGGAATAAAATTTGTAGGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGATGAATCTCAGTTTAAAACTTGGATGGCAATCGTCAATGATTCTACTCATGTCCATGCTCTTAAACCTGTTATTTACATAACCACATTAACATTCTGTGTCGATTAGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGATCTGACTATATCTTATTTGAGAGAATTCAATTTGTTCACTTGCTGCTATATTTTGCCTCTGTGCCCTTCGTTTCTTCTGTTGATACATATTCAGAATAGATTAGGTAGTTTTAACATATAGTATTGAGTTTCACATGAACTGCTACAAGCTGTAATGTTTTTTGAGCAAATATATAAGTCATAAATCTATTTTATTTTACTTTTCAA

Coding sequence (CDS)

ATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGA

Protein sequence

MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW

Homology

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BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.3e-86
Identity = 148/199 (74.37%), Postives = 171/199 (85.93%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTA+IKSID KHLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+  ++DFASVH
Sbjct: 234 MTAFIKSIDNKHLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVH 293

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQ FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QRD F
Sbjct: 294 IYPDHWFHDQGFEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRF 353

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           Y+T++DVIYKSAKR RSGAG+L WQFL+EGME  NDDFGIVPWE+ SI +L+IEQSCRL+
Sbjct: 354 YRTIFDVIYKSAKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLS 413

Query: 181 RIGGDTQQLKALKYVCAQR 200
           RI G     K    +C+ R
Sbjct: 414 RITGRHLLDKKSIEMCSHR 432

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BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 7.3e-80
Identity = 136/199 (68.34%), Postives = 167/199 (83.92%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MT +IKSID KHLLTVGLEGFYGPNSPK  TVNPE+WAS+LG+DF++NS   ++DFASVH
Sbjct: 233 MTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVH 292

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFH+Q FE++LKFV KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QRD F
Sbjct: 293 IYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKF 352

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           Y+ ++DV+YKSAKR +SGAG+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL 
Sbjct: 353 YRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLG 412

Query: 181 RIGGDTQQLKALKYVCAQR 200
           ++ G  ++ K LK +C+ R
Sbjct: 413 KVTGRLKEQK-LKELCSHR 430

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BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 6.4e-60
Identity = 111/200 (55.50%), Postives = 146/200 (73.00%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGP-NSPKKSTVNPEEW-ASRLGSDFIRNSEIQHVDFAS 60
           M+AY+KSIDKKHLLTVG EGFYGP +S +K  +NP EW  +  G+DFIRNS+IQ +DFAS
Sbjct: 246 MSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQDIDFAS 305

Query: 61  VHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD 120
           VH+YPD+W      +++LKF+++W+ +H+EDGD EL+KPV+ TEFGLS   +GF  A RD
Sbjct: 306 VHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAHRD 365

Query: 121 SFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCR 180
             Y+ VYD+++ SA+R  +  G+L WQ   EGMEE +D F IVP ER S+ +LI EQSCR
Sbjct: 366 VLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQSCR 425

Query: 181 LARIG-GDTQQLKALKYVCA 198
           LA +  G+    K LK VCA
Sbjct: 426 LAAVRYGEEGARKVLKTVCA 445

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BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 6.7e-49
Identity = 92/188 (48.94%), Postives = 126/188 (67.02%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           M AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDFI+NS ++H+DFASVH
Sbjct: 243 MAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVH 302

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL---SDLNKGFTPAQR 120
            YPD W      E++++++S W+ SH+ D ++ LKKPV+FTE G    SD N   T   R
Sbjct: 303 AYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VDR 362

Query: 121 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 180
           D   + VYD IY SA++ ++G+G+L WQ +VEG     D+F +V  +R S Y LI  QSC
Sbjct: 363 DIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQSC 422

Query: 181 RLARIGGD 186
           RL R+ G+
Sbjct: 423 RLQRLYGE 429

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.4e-43
Identity = 83/182 (45.60%), Postives = 116/182 (63.74%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           M A++KS+D+ HLL  GLEGFYG +SP+  T+NP     + G+DFI N+ I  +DF +VH
Sbjct: 222 MAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFIANNRIPGIDFVTVH 281

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
            YPD WF D   + ++ F++KW+ +HI+D    L KP++  EFG S    G+TPAQRD  
Sbjct: 282 SYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRDIV 341

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           + TVY  IY SAKR  + AG L WQ LV G++   D +GI+  + SS   +I +QS +L 
Sbjct: 342 FNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRKLT 400

Query: 181 RI 183
            I
Sbjct: 402 LI 400

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BLAST of Cucsat.G18215.T2 vs. NCBI nr
Match: XP_004139946.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical protein Csa_021081 [Cucumis sativus])

HSP 1 Score: 418 bits (1074), Expect = 4.17e-144
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGDTQQLKALKYVCAQRW
Sbjct: 412 RIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. NCBI nr
Match: XP_008448293.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 410 bits (1053), Expect = 6.46e-141
Identity = 197/200 (98.50%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGDTQQLKALKYVCAQRW
Sbjct: 412 RIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. NCBI nr
Match: KAA0062055.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 409 bits (1050), Expect = 1.85e-140
Identity = 196/200 (98.00%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           +IGGDTQQLKALKYVCAQRW
Sbjct: 412 KIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. NCBI nr
Match: XP_038902035.1 (mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida])

HSP 1 Score: 398 bits (1022), Expect = 3.30e-136
Identity = 191/200 (95.50%), Postives = 193/200 (96.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEI +VDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPNVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFTPAQRD F
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERQSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGD QQLKALKYVCAQRW
Sbjct: 412 RIGGDAQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. NCBI nr
Match: XP_023512795.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392 bits (1008), Expect = 7.73e-135
Identity = 188/196 (95.92%), Postives = 191/196 (97.45%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           M+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI H+DFASVH
Sbjct: 184 MSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVH 243

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD F
Sbjct: 244 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRF 303

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLA
Sbjct: 304 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLA 363

Query: 181 RIGGDTQQLKALKYVC 196
           RIGGD QQLKALK VC
Sbjct: 364 RIGGDIQQLKALKNVC 379

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 2.02e-144
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGDTQQLKALKYVCAQRW
Sbjct: 412 RIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 3.13e-141
Identity = 197/200 (98.50%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGDTQQLKALKYVCAQRW
Sbjct: 412 RIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 3.13e-141
Identity = 197/200 (98.50%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           RIGGDTQQLKALKYVCAQRW
Sbjct: 412 RIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL
Match: A0A5A7V836 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003960 PE=3 SV=1)

HSP 1 Score: 409 bits (1050), Expect = 8.94e-141
Identity = 196/200 (98.00%), Postives = 197/200 (98.50%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFASVH
Sbjct: 232 MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVH 291

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQRDSF
Sbjct: 292 IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSF 351

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA
Sbjct: 352 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 411

Query: 181 RIGGDTQQLKALKYVCAQRW 200
           +IGGDTQQLKALKYVCAQRW
Sbjct: 412 KIGGDTQQLKALKYVCAQRW 431

[Close]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL
Match: A0A6J1FWH9 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 1.52e-134
Identity = 187/196 (95.41%), Postives = 191/196 (97.45%), Query Frame = 0


Query: 1   MTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVH 60
           M+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI H+DFASVH
Sbjct: 184 MSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFASVH 243

Query: 61  IYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSF 120
           IYPDHWFHDQDFE+ELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD F
Sbjct: 244 IYPDHWFHDQDFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDRF 303

Query: 121 YKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLA 180
           YKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSCRLA
Sbjct: 304 YKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSCRLA 363

Query: 181 RIGGDTQQLKALKYVC 196
           RIGGD QQLKALK VC
Sbjct: 364 RIGGDIQQLKALKNVC 379

The following BLAST results are available for this feature:

BLAST of Cucsat.G18215.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7Y223	2.3e-86	74.37	Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ...	[more]
Q9M0H6	7.3e-80	68.34	Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ...	[more]
Q0JJD4	6.4e-60	55.50	Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q0DCM5	6.7e-49	48.94	Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FJZ3	1.4e-43	45.60	Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ...	[more]

BLAST of Cucsat.G18215.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139946.1	4.17e-144	100.00	mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical pr...	[more]
XP_008448293.1	6.46e-141	98.50	PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan ...	[more]
KAA0062055.1	1.85e-140	98.00	mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa]	[more]
XP_038902035.1	3.30e-136	95.50	mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida]	[more]
XP_023512795.1	7.73e-135	95.92	mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G18215.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB35	2.02e-144	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3...	[more]
A0A5D3C3G0	3.13e-141	98.50	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BJC2	3.13e-141	98.50	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV...	[more]
A0A5A7V836	8.94e-141	98.00	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1FWH9	1.52e-134	95.41	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..183 e-value: 4.3E-50 score: 172.7
None	No IPR available	PANTHER	PTHR31451:SF61	BNAA09G43620D PROTEIN	coord: 1..198
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 1..198
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..167

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G18215	Cucsat.G18215	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T2.E1	Cucsat.G18215.T2.E1	exon
Cucsat.G18215.T2.E2	Cucsat.G18215.T2.E2	exon
Cucsat.G18215.T2.E3	Cucsat.G18215.T2.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T2.C2	Cucsat.G18215.T2.C2	CDS
Cucsat.G18215.T2.C1	Cucsat.G18215.T2.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G18215.T2	Cucsat.G18215.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity