Cucsat.G6069.T7 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G6069.T7
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Protein-S-isoprenylcysteine O-methyltransferase
Location	ctg1425: 1895961 .. 1897332 (-)
RNA-Seq Expression	Cucsat.G6069.T7
Synteny	Cucsat.G6069.T7

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTCCCTTCTGAATTACGTCAGACGAACCACACAAACTCTTGGCTTCCAGATCTGAGGCGCTCTCTCGCTACAAGCCTACAAAGCCTCCCAACGGTGAACGACGCAACCTTCAGGTCAGGTGTAACCCTAACCAACTACCAACCCTCGGTAGGTAATGGTAATAATACATATTTTCTTTTGAATTTTCAATGACGAATATTTTAAGGTTTTCTTTTTCTTACTTTCCTTCGAAGACCTTAACTTCTCATCTCTTCTTCTAGGGTTTTTCTTTTCACATCTATCTTCCAAAAATCAAACGTCTCATTTGCCGCCCACAACTCCAAGAGGAGATTAGTAAAATCTTTAGTATCCATCCCTGATACTTTGTTTTACGAACCTGGTTTAATTGTTCACATATCAGATGTTTTGCATAAAGGATCTCCAGCGTTTTCTACACAAGAATTTCCCTGTCTTGGTGTTATATGGCTCTGCATCATTTTCAGGTCTGAACTAAGATCCTGTAATACATTTTGAATTCTTTTTTGGTGTGGGAGAGGCCGTATCTCATACTTTGCCGTCAGAAAACATGACAGAAATACTCAGTTACACCGCATGCAGACAGTTGTCTCAGATGGTTTCTGCAATCATCTTCTTTCACGCTTCTGAATATATCTTAGCAGTTGGCATTCATGGGAGATCCAATGTTACTCTAAAGTCCCTTCTGATTAGCAAAAACTACCTCTTGGCAATGATCTTATCTTTCTTAGAGTACTTCCTTGAAATTGTGTTGTGTCCCAGTCTGAAGGAATACTGGTGGGTTAGTCAGACAGGCCTTGCAATGGTTGTAGTTGGAGAAATCGTCCGTAAACTGGCCATCATAACTGCAGGCCGGTGTTTTACACATCTGATCAAGATCTATCATGAGGACCATCACAACCTGGTTACTCATGGAGTCTATAGCTTTGTCCGTCATCCTGGATACAGTGGTTTCCTCGTGTGGGCAGTTGGCACTCAAATCATGCTCTGTAATCCCATTTCAACCATTGCATTTGCTGTTGTGGTTTGGCACTTCTTTGCTGAACGGATTCCGTATGAAGAGTACTTCTTGAGACAGTTTTTTGGCCATGAGTATGAGGAGTACGCGAATCGGGTATCTTCTGGAGTGCCATTTGTGAAATGAGCTTGGACTGATGATTTTGATGCTTCATCAACAGAGTTCATGATTCTGTCCTTGAGGTATTTGGAACTTTTTAGGGGAAGTAGTTTTTGTGTTGTATATTAAGGCTACTGATTGATGATTTTTGGGATTGCTTCTATGGTTAACAAGCCTTTGGATCTACTTGTAACATTTTTGTCAGTTATAAAGGAAATATATATCATTTAAACTCCCTT

Coding sequence (CDS)

CTCCCTTCTGAATTACGTCAGACGAACCACACAAACTCTTGGCTTCCAGATCTGAGGCGCTCTCTCGCTACAAGCCTACAAAGCCTCCCAACGGTGAACGACGCAACCTTCAGGTCAGGTGTAACCCTAACCAACTACCAACCCTCGATGTTTTGCATAAAGGATCTCCAGCGTTTTCTACACAAGAATTTCCCTGTCTTGGTGTTATATGGCTCTGCATCATTTTCAGAAATACTCAGTTACACCGCATGCAGACAGTTGTCTCAGATGGTTTCTGCAATCATCTTCTTTCACGCTTCTGAATATATCTTAGCAGTTGGCATTCATGGGAGATCCAATGTTACTCTAAAGTCCCTTCTGATTAGCAAAAACTACCTCTTGGCAATGATCTTATCTTTCTTAGAGTACTTCCTTGAAATTGTGTTGTGTCCCAGTCTGAAGGAATACTGGTGGGTTAGTCAGACAGGCCTTGCAATGGTTGTAGTTGGAGAAATCGTCCGTAAACTGGCCATCATAACTGCAGGCCGGTGTTTTACACATCTGATCAAGATCTATCATGAGGACCATCACAACCTGGTTACTCATGGAGTCTATAGCTTTGTCCGTCATCCTGGATACAGTGGTTTCCTCGTGTGGGCAGTTGGCACTCAAATCATGCTCTGTAATCCCATTTCAACCATTGCATTTGCTGTTGTGGTTTGGCACTTCTTTGCTGAACGGATTCCGTATGAAGAGTACTTCTTGAGACAGTTTTTTGGCCATGAGTATGAGGAGTACGCGAATCGGGTATCTTCTGGAGTGCCATTTGTGAAATGA

Protein sequence

LPSELRQTNHTNSWLPDLRRSLATSLQSLPTVNDATFRSGVTLTNYQPSMFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK

Homology

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BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot
Match: Q9FMW9 (Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=3702 GN=ICMTA PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 8.7e-76
Identity = 137/195 (70.26%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI S T+ RQLSQM+ ++IFFH SEYILA+ IHG SNVTL SLLI+K+Y LAM+LS LE
Sbjct: 2   TEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           Y  EI+L P LK++WWVS  GL M++VGEI+RK AIITAGR FTHLIKI +E+HH LVTH
Sbjct: 62  YLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTH 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVY  +RHP Y GFL+W+VGTQ+MLCNP+S +AFAVVVW FFA+RIPYEEYFL QFFG +
Sbjct: 122 GVYRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGVQ 181

Query: 256 YEEYANRVSSGVPFV 271
           Y EYA  V+SGVPFV
Sbjct: 182 YLEYAESVASGVPFV 196

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot
Match: Q93W54 (Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=3702 GN=ICMTB PE=1 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-74
Identity = 137/195 (70.26%), Postives = 159/195 (81.54%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI S T  RQL+QM  AIIFFH SEYILA+ IHG S VTL SLLISK+Y LAM++S LE
Sbjct: 2   TEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           Y  EIV  P LK++WW+S  GL M+++GEI+RK AIITAGR FTHLIKI  E+HH LVT 
Sbjct: 62  YIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTE 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVY  +RHP YSGFL+W+VGTQ+MLCNPIS IAFAVVVW FFAERIPYEE++L+QFFG +
Sbjct: 122 GVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGRQ 181

Query: 256 YEEYANRVSSGVPFV 271
           Y EYA RV SGVPFV
Sbjct: 182 YVEYAQRVPSGVPFV 196

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot
Match: A2XX73 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=ICMT PE=3 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 1.8e-68
Identity = 116/180 (64.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 92  SAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWW 151
           +A++ FH SEY+LA   HGR NVT  SLLISK Y+LAM  + LE+  E +L P LKEYW+
Sbjct: 12  AALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWF 71

Query: 152 VSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLV 211
           VS  GL MV++GE++RKLA++TAGR FTH+I+I++ED H L+THGVY  +RHPGYSGFL+
Sbjct: 72  VSYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLI 131

Query: 212 WAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           WAVGTQ+MLCNP+ST+AF +V+W FF++RIPYEE+FLRQFFG EYEEYA +V SG+PF++
Sbjct: 132 WAVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot
Match: Q7XSR9 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=ICMT PE=3 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 1.8e-68
Identity = 116/180 (64.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 92  SAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWW 151
           +A++ FH SEY+LA   HGR NVT  SLLISK Y+LAM  + LE+  E +L P LKEYW+
Sbjct: 12  AALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWF 71

Query: 152 VSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLV 211
           VS  GL MV++GE++RKLA++TAGR FTH+I+I++ED H L+THGVY  +RHPGYSGFL+
Sbjct: 72  VSYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLI 131

Query: 212 WAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           WAVGTQ+MLCNP+ST+AF +V+W FF++RIPYEE+FLRQFFG EYEEYA +V SG+PF++
Sbjct: 132 WAVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot
Match: O60725 (Protein-S-isoprenylcysteine O-methyltransferase OS=Homo sapiens OX=9606 GN=ICMT PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-38
Identity = 83/181 (45.86%), Postives = 117/181 (64.64%), Query Frame = 0


Query: 93  AIIFFHASEYILAVGIHGRSNVTLKSLLI--SKNYLLAMILSFLEYFLEIVLCPSLKEYW 152
           ++  FH SEY L   ++   +++L S L+  S  Y +A + S+LE+ LE +  P LK+  
Sbjct: 99  SLSLFHYSEY-LVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQIT 158

Query: 153 WVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFL 212
           W+S TGL MVV GE +RK A+ TAG  F H+++    D H LVT GVY++ RHP Y G+ 
Sbjct: 159 WLSVTGLLMVVFGECLRKAAMFTAGSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWF 218

Query: 213 VWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFV 272
            W++GTQ+MLCNPI  +++A+ VW FF +R   EE  L  FFG EY EY  RV +G+PF+
Sbjct: 219 YWSIGTQVMLCNPICGVSYALTVWRFFRDRTEEEEISLIHFFGEEYLEYKKRVPTGLPFI 278

[Close]

BLAST of Cucsat.G6069.T7 vs. NCBI nr
Match: XP_031744061.1 (protein-S-isoprenylcysteine O-methyltransferase A isoform X2 [Cucumis sativus])

HSP 1 Score: 444 bits (1143), Expect = 1.47e-156
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH
Sbjct: 1   MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL
Sbjct: 61  GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF
Sbjct: 121 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK
Sbjct: 181 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 222

[Close]

BLAST of Cucsat.G6069.T7 vs. NCBI nr
Match: XP_031744058.1 (protein-S-isoprenylcysteine O-methyltransferase A isoform X1 [Cucumis sativus] >XP_031744059.1 protein-S-isoprenylcysteine O-methyltransferase A isoform X1 [Cucumis sativus] >XP_031744060.1 protein-S-isoprenylcysteine O-methyltransferase A isoform X1 [Cucumis sativus] >KAE8646690.1 hypothetical protein Csa_005377 [Cucumis sativus])

HSP 1 Score: 439 bits (1128), Expect = 3.27e-154
Identity = 222/226 (98.23%), Postives = 222/226 (98.23%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSE----ILSYTACRQLSQMVSAIIFFHASEYILA 109
           MFCIKDLQRFLHKNFPVLVLYGSASFSE    ILSYTACRQLSQMVSAIIFFHASEYILA
Sbjct: 1   MFCIKDLQRFLHKNFPVLVLYGSASFSENMTEILSYTACRQLSQMVSAIIFFHASEYILA 60

Query: 110 VGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEI 169
           VGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEI
Sbjct: 61  VGIHGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEI 120

Query: 170 VRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPIS 229
           VRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPIS
Sbjct: 121 VRKLAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPIS 180

Query: 230 TIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           TIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK
Sbjct: 181 TIAFAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 226

[Close]

BLAST of Cucsat.G6069.T7 vs. NCBI nr
Match: XP_038877249.1 (protein-S-isoprenylcysteine O-methyltransferase B-like isoform X2 [Benincasa hispida])

HSP 1 Score: 418 bits (1074), Expect = 5.53e-146
Identity = 207/223 (92.83%), Postives = 213/223 (95.52%), Query Frame = 0


Query: 49  SMFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGI 108
           SM CIKDLQRF+HKNF VLVLYGSASFSEI SYTACR LSQM+ AIIFFH+SEYILAVGI
Sbjct: 4   SMLCIKDLQRFVHKNFCVLVLYGSASFSEIFSYTACRHLSQMMFAIIFFHSSEYILAVGI 63

Query: 109 HGRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRK 168
           HGRSNVTL SLLISKNYLLAMILSFLEYFLEI+LCPSLKEYWWVSQTGLAMVVVGEI+RK
Sbjct: 64  HGRSNVTLTSLLISKNYLLAMILSFLEYFLEIILCPSLKEYWWVSQTGLAMVVVGEIIRK 123

Query: 169 LAIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIA 228
           LAIITAGRCFTH IKIYHEDHH LVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNP STIA
Sbjct: 124 LAIITAGRCFTHRIKIYHEDHHKLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPFSTIA 183

Query: 229 FAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           FAVVVWHFFAERIPYEEYFLRQFFGHEYEEYAN+V SGVPFVK
Sbjct: 184 FAVVVWHFFAERIPYEEYFLRQFFGHEYEEYANQVPSGVPFVK 226

[Close]

BLAST of Cucsat.G6069.T7 vs. NCBI nr
Match: XP_022156108.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Momordica charantia])

HSP 1 Score: 401 bits (1031), Expect = 1.70e-139
Identity = 196/222 (88.29%), Postives = 208/222 (93.69%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           M C+KDLQ F+HKNF VLVLY SASF EI SYTACRQLSQM+ AIIFFH SEYILAVGIH
Sbjct: 1   MLCLKDLQDFVHKNFSVLVLYSSASFPEIFSYTACRQLSQMIFAIIFFHISEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GRSNVTL SLLISKNY+LAM+LSFLEYFLEI+LCP LKEYWWVSQTGLAMVV+GEIVRKL
Sbjct: 61  GRSNVTLNSLLISKNYILAMVLSFLEYFLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGR FTHLIKIYHEDHH LVT+GVY FVRHPGYSGFLVWAVGTQIMLCNP+ST+AF
Sbjct: 121 AIITAGRSFTHLIKIYHEDHHKLVTYGVYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           AVVVWHFFAERIPYEEY+LR+FFGHEY EYANRVSSGVPFVK
Sbjct: 181 AVVVWHFFAERIPYEEYYLRRFFGHEYVEYANRVSSGVPFVK 222

[Close]

BLAST of Cucsat.G6069.T7 vs. NCBI nr
Match: XP_023531975.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388 bits (997), Expect = 2.22e-134
Identity = 193/222 (86.94%), Postives = 202/222 (90.99%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           M C+KDLQ FLHKNF  L +    SFSEI SYTACRQLSQM+ AIIFFH SEYILAVGIH
Sbjct: 1   MLCLKDLQGFLHKNFSELEI----SFSEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GR NVTLKSLLISKNYLLAMILSFLEYFLE++LCPSLKEYWWVS  GLAMVVVGEIVRKL
Sbjct: 61  GRQNVTLKSLLISKNYLLAMILSFLEYFLEVLLCPSLKEYWWVSHIGLAMVVVGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGR FTHLIK+YHEDHH LVTHGVY FVRHPGY+GFLVWAVGTQIMLCNPIST+AF
Sbjct: 121 AIITAGRSFTHLIKVYHEDHHKLVTHGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           A+VVWHFFAERIPYEEYFLRQFFGHEYEEYA RV SGVPFVK
Sbjct: 181 AIVVWHFFAERIPYEEYFLRQFFGHEYEEYARRVPSGVPFVK 218

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL
Match: A0A6J1DPQ1 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111023074 PE=3 SV=1)

HSP 1 Score: 401 bits (1031), Expect = 8.23e-140
Identity = 196/222 (88.29%), Postives = 208/222 (93.69%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           M C+KDLQ F+HKNF VLVLY SASF EI SYTACRQLSQM+ AIIFFH SEYILAVGIH
Sbjct: 1   MLCLKDLQDFVHKNFSVLVLYSSASFPEIFSYTACRQLSQMIFAIIFFHISEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GRSNVTL SLLISKNY+LAM+LSFLEYFLEI+LCP LKEYWWVSQTGLAMVV+GEIVRKL
Sbjct: 61  GRSNVTLNSLLISKNYILAMVLSFLEYFLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGR FTHLIKIYHEDHH LVT+GVY FVRHPGYSGFLVWAVGTQIMLCNP+ST+AF
Sbjct: 121 AIITAGRSFTHLIKIYHEDHHKLVTYGVYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           AVVVWHFFAERIPYEEY+LR+FFGHEY EYANRVSSGVPFVK
Sbjct: 181 AVVVWHFFAERIPYEEYYLRRFFGHEYVEYANRVSSGVPFVK 222

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL
Match: A0A6J1EK92 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435324 PE=3 SV=1)

HSP 1 Score: 385 bits (990), Expect = 1.25e-133
Identity = 191/222 (86.04%), Postives = 201/222 (90.54%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           M C+KDLQ FLHKNF  L +    SFSEI SYTACRQLSQM+ AIIFFH SEYILAVGIH
Sbjct: 1   MLCLKDLQGFLHKNFSELEI----SFSEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GR NVTLKSLLISKNYLLAMILSF+EYFLE+ LCP+LKEYWWVS  GLAMVVVGEIVRKL
Sbjct: 61  GRQNVTLKSLLISKNYLLAMILSFIEYFLEVFLCPTLKEYWWVSHIGLAMVVVGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGR FTHLIK+YHEDHH LVTHGVY FVRHPGY+GFLVWAVGTQIMLCNPIST+AF
Sbjct: 121 AIITAGRSFTHLIKVYHEDHHKLVTHGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           A+VVWHFFAERIPYEEYFLRQFFGHEYEEYA RV SGVPFVK
Sbjct: 181 AIVVWHFFAERIPYEEYFLRQFFGHEYEEYARRVPSGVPFVK 218

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL
Match: A0A1S3CC02 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499053 PE=3 SV=1)

HSP 1 Score: 382 bits (982), Expect = 9.80e-133
Identity = 190/196 (96.94%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI SYTACRQLSQMV+AIIFFH SEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE
Sbjct: 2   TEIFSYTACRQLSQMVAAIIFFHTSEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHH+LVTH
Sbjct: 62  YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHHLVTH 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVYSFVRHPGYSGFL+WAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE
Sbjct: 122 GVYSFVRHPGYSGFLMWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 181

Query: 256 YEEYANRVSSGVPFVK 271
           YEEYANRVSSGVPFVK
Sbjct: 182 YEEYANRVSSGVPFVK 197

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL
Match: A0A5D3DLI6 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00160 PE=3 SV=1)

HSP 1 Score: 382 bits (982), Expect = 9.80e-133
Identity = 190/196 (96.94%), Postives = 194/196 (98.98%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI SYTACRQLSQMV+AIIFFH SEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE
Sbjct: 2   TEIFSYTACRQLSQMVAAIIFFHTSEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHH+LVTH
Sbjct: 62  YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHHLVTH 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVYSFVRHPGYSGFL+WAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE
Sbjct: 122 GVYSFVRHPGYSGFLMWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 181

Query: 256 YEEYANRVSSGVPFVK 271
           YEEYANRVSSGVPFVK
Sbjct: 182 YEEYANRVSSGVPFVK 197

[Close]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL
Match: A0A6J1JPG8 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486321 PE=3 SV=1)

HSP 1 Score: 377 bits (969), Expect = 1.98e-130
Identity = 189/222 (85.14%), Postives = 200/222 (90.09%), Query Frame = 0


Query: 50  MFCIKDLQRFLHKNFPVLVLYGSASFSEILSYTACRQLSQMVSAIIFFHASEYILAVGIH 109
           M  +KDLQ FL KNF  L +    SFSEI SYTACRQLSQM+ AIIFFH SEYILAVGIH
Sbjct: 1   MLFLKDLQGFLLKNFSELEI----SFSEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIH 60

Query: 110 GRSNVTLKSLLISKNYLLAMILSFLEYFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKL 169
           GR NVTLKSLLISKNYLLAMILSFLEYFLE+++CP+LKEYWWVS  GLAMVVVGEIVRKL
Sbjct: 61  GRQNVTLKSLLISKNYLLAMILSFLEYFLEVLVCPNLKEYWWVSHIGLAMVVVGEIVRKL 120

Query: 170 AIITAGRCFTHLIKIYHEDHHNLVTHGVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAF 229
           AIITAGR FTHLIK+YHEDHH LVTHGVY FVRHPGY+GFLVWAVGTQIMLCNPIST+AF
Sbjct: 121 AIITAGRSFTHLIKVYHEDHHKLVTHGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAF 180

Query: 230 AVVVWHFFAERIPYEEYFLRQFFGHEYEEYANRVSSGVPFVK 271
           A+VVWHFFAERIPYEEYFLRQFFGHEYEEYA RV SGVPFVK
Sbjct: 181 AIVVWHFFAERIPYEEYFLRQFFGHEYEEYARRVPSGVPFVK 218

[Close]

BLAST of Cucsat.G6069.T7 vs. TAIR 10
Match: AT5G23320.1 (homolog of yeast STE14 A )

HSP 1 Score: 285.0 bits (728), Expect = 6.2e-77
Identity = 137/195 (70.26%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI S T+ RQLSQM+ ++IFFH SEYILA+ IHG SNVTL SLLI+K+Y LAM+LS LE
Sbjct: 2   TEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           Y  EI+L P LK++WWVS  GL M++VGEI+RK AIITAGR FTHLIKI +E+HH LVTH
Sbjct: 62  YLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTH 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVY  +RHP Y GFL+W+VGTQ+MLCNP+S +AFAVVVW FFA+RIPYEEYFL QFFG +
Sbjct: 122 GVYRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGVQ 181

Query: 256 YEEYANRVSSGVPFV 271
           Y EYA  V+SGVPFV
Sbjct: 182 YLEYAESVASGVPFV 196

[Close]

BLAST of Cucsat.G6069.T7 vs. TAIR 10
Match: AT5G08335.1 (Isoprenylcysteine carboxyl methyltransferase (ICMT) family )

HSP 1 Score: 281.2 bits (718), Expect = 8.9e-76
Identity = 137/195 (70.26%), Postives = 159/195 (81.54%), Query Frame = 0


Query: 76  SEILSYTACRQLSQMVSAIIFFHASEYILAVGIHGRSNVTLKSLLISKNYLLAMILSFLE 135
           +EI S T  RQL+QM  AIIFFH SEYILA+ IHG S VTL SLLISK+Y LAM++S LE
Sbjct: 2   TEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLE 61

Query: 136 YFLEIVLCPSLKEYWWVSQTGLAMVVVGEIVRKLAIITAGRCFTHLIKIYHEDHHNLVTH 195
           Y  EIV  P LK++WW+S  GL M+++GEI+RK AIITAGR FTHLIKI  E+HH LVT 
Sbjct: 62  YIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTE 121

Query: 196 GVYSFVRHPGYSGFLVWAVGTQIMLCNPISTIAFAVVVWHFFAERIPYEEYFLRQFFGHE 255
           GVY  +RHP YSGFL+W+VGTQ+MLCNPIS IAFAVVVW FFAERIPYEE++L+QFFG +
Sbjct: 122 GVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGRQ 181

Query: 256 YEEYANRVSSGVPFV 271
           Y EYA RV SGVPFV
Sbjct: 182 YVEYAQRVPSGVPFV 196

The following BLAST results are available for this feature:

BLAST of Cucsat.G6069.T7 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMW9	8.7e-76	70.26	Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=370...	[more]
Q93W54	1.3e-74	70.26	Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=370...	[more]
A2XX73	1.8e-68	64.44	Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ...	[more]
Q7XSR9	1.8e-68	64.44	Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ...	[more]
O60725	1.4e-38	45.86	Protein-S-isoprenylcysteine O-methyltransferase OS=Homo sapiens OX=9606 GN=ICMT ...	[more]

BLAST of Cucsat.G6069.T7 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031744061.1	1.47e-156	100.00	protein-S-isoprenylcysteine O-methyltransferase A isoform X2 [Cucumis sativus]	[more]
XP_031744058.1	3.27e-154	98.23	protein-S-isoprenylcysteine O-methyltransferase A isoform X1 [Cucumis sativus] >...	[more]
XP_038877249.1	5.53e-146	92.83	protein-S-isoprenylcysteine O-methyltransferase B-like isoform X2 [Benincasa his...	[more]
XP_022156108.1	1.70e-139	88.29	protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Momordica cha...	[more]
XP_023531975.1	2.22e-134	86.94	protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Cucurbita pep...	[more]

BLAST of Cucsat.G6069.T7 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPQ1	8.23e-140	88.29	Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G...	[more]
A0A6J1EK92	1.25e-133	86.04	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN...	[more]
A0A1S3CC02	9.80e-133	96.94	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3DLI6	9.80e-133	96.94	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A6J1JPG8	1.98e-130	85.14	Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Cucsat.G6069.T7 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23320.1	6.2e-77	70.26	homolog of yeast STE14 A	[more]
AT5G08335.1	8.9e-76	70.26	Isoprenylcysteine carboxyl methyltransferase (ICMT) family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 72..270 e-value: 5.9E-36 score: 126.0
None	No IPR available	PANTHER	PTHR12714	PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE	coord: 48..270
None	No IPR available	PANTHER	PTHR12714:SF22	PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE	coord: 48..270
IPR007269	Isoprenylcysteine carboxyl methyltransferase	PFAM	PF04140	ICMT	coord: 157..248 e-value: 2.7E-27 score: 95.0
IPR025770	Protein-S-isoprenylcysteine O-methyltransferase	PROSITE	PS51564	SAM_ICMT	coord: 3..271 score: 28.635201

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G6069	Cucsat.G6069	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6069.T7.E1	Cucsat.G6069.T7.E1	exon
Cucsat.G6069.T7.E2	Cucsat.G6069.T7.E2	exon
Cucsat.G6069.T7.E3	Cucsat.G6069.T7.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6069.T7.C3	Cucsat.G6069.T7.C3	CDS
Cucsat.G6069.T7.C2	Cucsat.G6069.T7.C2	CDS
Cucsat.G6069.T7.C1	Cucsat.G6069.T7.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6069.T7	Cucsat.G6069.T7-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006481	C-terminal protein methylation
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0004671	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity