Cp4.1LG01g16820 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g16820 vs. ExPASy Swiss-Prot
Match: F4HRT5 (Protein CROWDED NUCLEI 1 OS=Arabidopsis thaliana OX=3702 GN=CRWN1 PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.0e-154
Identity = 412/1119 (36.82%), Postives = 654/1119 (58.45%), Query Frame = 0

Query: 59   DREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLI 118
            D   L EK S L+ ELF+YQH++GL+L+EKK W+S+Y+ L Q   E  E  K+E++AHLI
Sbjct: 48   DPRILPEKISELEKELFEYQHSMGLLLIEKKEWSSQYEALQQAFEEVNECLKQERNAHLI 107

Query: 119  ALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEE 178
            A+++VE R + L+KAL  EKQ    L+KAL E++ E AEIK T+  KL +ANAL+  +EE
Sbjct: 108  AIADVEKREEGLRKALGIEKQCALDLEKALKELRAENAEIKFTADSKLTEANALVRSVEE 167

Query: 179  KSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHK 238
            KSLE++ KL A + KLAEV+RKSS++E +  EVEARES LQ E+ S +  +EA + T  K
Sbjct: 168  KSLEVEAKLRAVDAKLAEVSRKSSDVERKAKEVEARESSLQRERFSYIAEREADEATLSK 227

Query: 239  EKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSI 298
            +++ LR+W++KLQE EE +++S+ ++  +E + NE+  I+KQK K+LEE +KKID ++  
Sbjct: 228  QREDLREWERKLQEGEERVAKSQMIVKQREDRANESDKIIKQKGKELEEAQKKIDAANLA 287

Query: 299  LKEKEDDVNRQLAD----------------------------VEAKEKMETQQLLHEQSA 358
            +K+ EDDV+ ++ D                            +EA+EKM  QQL+ E  A
Sbjct: 288  VKKLEDDVSSRIKDLALREQETDVLKKSIETKARELQALQEKLEAREKMAVQQLVDEHQA 347

Query: 359  LLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTK 418
             L   + EFEL++E+KR+S+++     +  +++++ E  H +EK+ K+EQALD+KL + K
Sbjct: 348  KLDSTQREFELEMEQKRKSIDDSLKSKVAEVEKREAEWKHMEEKVAKREQALDRKLEKHK 407

Query: 419  EKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEW 478
            EKE D + +LK +  ++K LK++E+  E E+ ++L D++ + +L   +EK+   +  +  
Sbjct: 408  EKENDFDLRLKGISGREKALKSEEKALETEKKKLLEDKEIILNLKALVEKVSGENQAQLS 467

Query: 479  QFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEA 538
            + ++E+++L+V +EERSE++RLQ +L ++IE  R Q +++ KE +DLK +R  FE++WE 
Sbjct: 468  EINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQELLQKEAEDLKAQRESFEKEWEE 527

Query: 539  LDEKRTEIHNGLGDLEELRKKLE-ILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFAS 598
            LDE++ +I N L ++ + ++KLE  +  EEERL+ EK      M+REL+ ++  K  FA 
Sbjct: 528  LDERKAKIGNELKNITDQKEKLERHIHLEEERLKKEKQAANENMERELETLEVAKASFAE 587

Query: 599  TTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRE 658
            T  +E+  LS++A+++ +QLL DIE++++ LES +Q   EE E+  Q ++  FEEER++E
Sbjct: 588  TMEYERSMLSKKAESERSQLLHDIEMRKRKLESDMQTILEEKERELQAKKKLFEEEREKE 647

Query: 659  RNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKEL 718
             + I YL+D+A +E  ++ +ER ++EKEK  V  ++  L     EI +D+D L  L+K+L
Sbjct: 648  LSNINYLRDVARREMMDMQNERQRIEKEKLEVDSSKNHLEEQQTEIRKDVDDLVALTKKL 707

Query: 719  KCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPD---LQMPADIREPDPLAN-LDV 778
            K QREQ I +R  FL+ ++  ++C  CG  + E ++P+   L+MP        LAN LD 
Sbjct: 708  KEQREQFISERSRFLSSMESNRNCSRCGELLSELVLPEIDNLEMP----NMSKLANILDN 767

Query: 779  ESLKLFQKEL---AASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLA--- 838
            E+ +   +++   AA      +GG++SW R+C+ K+L LSPIK      P V+  LA   
Sbjct: 768  EAPRQEMRDISPTAAGLGLPVTGGKVSWFRKCTSKMLKLSPIK---MTEPSVTWNLADQE 827

Query: 839  ---ADCTDLEAKEPSVSAG-----DVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEE-A 898
                +  ++     +V A      DV++       +  E  + ++  D  +++SK +E A
Sbjct: 828  PQSTEQANVGGPSTTVQAATTYSFDVQKAESETGTKEVEVTNVNSDGDQSDINSKAQEVA 887

Query: 899  SEGSKQPDLKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSD--S 958
            ++     D+  + + +GKG     RT S+K  V DAK   GE++   + N   +  D  +
Sbjct: 888  ADSLSNLDVDGQSRMKGKGKARTRRTRSVKDVVDDAKALYGESINLYEPNDSTENVDDST 947

Query: 959  KFSNKET--------TNVRKRPRAESSTISVSEQDGDDSERCSDSITTG-RQRKRQQKIA 1018
            K S  ET         N RKR R  S     +EQDG++S+  SDS+T G  QRKR+QK+A
Sbjct: 948  KASTGETGRSDKAISKNGRKRGRVGSLRTCTTEQDGNESDGKSDSVTGGAHQRKRRQKVA 1007

Query: 1019 SVQAQGE---SRYNLRRPKIGGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSS 1078
            S Q QGE    RYNLRRP+      A         G+++E+    T       +A  T+S
Sbjct: 1008 SEQ-QGEVVGQRYNLRRPRRVTGEPALSKKNEDIGGVQQEEGIHCT-------QATATAS 1067

Query: 1079 LRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESLRNPEDNASMEKLVAMDDLCDEVNN 1116
            +     +N    + VQ         SED            D  S ++    + + ++VN 
Sbjct: 1068 VGVAVSDNGVSTNVVQHEATAD---SED-----------TDAGSPKRTDESEAMSEDVNK 1127

BLAST of Cp4.1LG01g16820 vs. ExPASy Swiss-Prot
Match: Q9CA42 (Protein CROWDED NUCLEI 3 OS=Arabidopsis thaliana OX=3702 GN=CRWN3 PE=1 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.1e-146
Identity = 416/1169 (35.59%), Postives = 661/1169 (56.54%), Query Frame = 0

Query: 1    MFTPQR----------KDVAFTDG--PPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAG 60
            MFTPQR          K +AF+D    PPP         ++    D    DDW+KFK+ G
Sbjct: 1    MFTPQRNRWPETDRKGKAIAFSDEIITPPP--------QRVLLRED----DDWQKFKEVG 60

Query: 61   LLDAAAMERKDREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEI 120
            LLD A++ERKDR+AL+EK  +L+ ELFDYQHN+GL+L+EKK W S  ++L Q   E  E+
Sbjct: 61   LLDEASLERKDRDALIEKILKLEKELFDYQHNMGLLLIEKKQWTSTNNELQQAYDEAMEM 120

Query: 121  FKREQSAHLIALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLAD 180
             KRE++++ I L+E + R +NL+KAL  EKQ V+ L+  L   + E + +K TS+ KL +
Sbjct: 121  LKREKTSNAITLNEADKREENLRKALIDEKQFVAELENDLKYWQREHSVVKSTSEAKLEE 180

Query: 181  ANALMHGIEEKSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTR 240
            ANAL+ G++EK+LE+ ++   AE K + +NRKSSELE ++ EVE RE V Q E +SLVT 
Sbjct: 181  ANALVIGMKEKALEVDRERAIAEEKFSVMNRKSSELERKLKEVETREKVHQREHLSLVTE 240

Query: 241  KEAHQVTSHKEKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEI 300
            +EAH+   +K+++ L++W++KL   E+ LS  +  +N +E++V EN   +++KEK LE +
Sbjct: 241  REAHEAVFYKQREDLQEWEKKLTLEEDRLSEVKRSINHREERVMENERTIEKKEKILENL 300

Query: 301  KKKIDQSSSILKEKEDDVNRQLADVEAKEK----------------------------ME 360
            ++KI  + S L EKE+ +  +L D+  KEK                            ME
Sbjct: 301  QQKISVAKSELTEKEESIKIKLNDISLKEKDFEAMKAKVDIKEKELHEFEENLIEREQME 360

Query: 361  TQQLLHEQSALLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQ 420
              +LL +Q A+L  +R EFE++LE+ R+S++ E       I++  +EI+HK+EKL K+E 
Sbjct: 361  IGKLLDDQKAVLDSRRREFEMELEQMRRSLDEELEGKKAEIEQLQVEISHKEEKLAKREA 420

Query: 421  ALDKKLHRTKEKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEK 480
            AL+KK    K+KE DL+ +LK +K K+K LKA+E+K  +E  ++L D++ L+ L DEIE+
Sbjct: 421  ALEKKEEGVKKKEKDLDARLKTVKEKEKALKAEEKKLHMENERLLEDKECLRKLKDEIEE 480

Query: 481  IRTASTQKEWQFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQE 540
            I T +T++E +  EE E L++ KEER E +RLQ +L Q+I+  + + +++ KE ++LKQ+
Sbjct: 481  IGTETTKQESRIREEHESLRITKEERVEFLRLQSELKQQIDKVKQEEELLLKEREELKQD 540

Query: 541  RVKFERDWEALDEKRTEIHNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDN 600
            + +FE++WEALD+KR  I     ++ E  +KL  L+ +E+ RL+ E+      ++RELD 
Sbjct: 541  KERFEKEWEALDKKRANITREQNEVAEENEKLRNLQISEKHRLKREEMTSRDNLKRELDG 600

Query: 601  VKQEKELFASTTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERE 660
            VK +KE F +                    ++D+E+Q+++L+   Q+ +E  E+   ER 
Sbjct: 601  VKMQKESFEAD-------------------MEDLEMQKRNLDMEFQRQEEAGERDFNERA 660

Query: 661  LAFEEERKRERNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDI 720
              +E+  + E + I Y K +A +E EE+  E+  LE+E+E +++ +K L     E+H+DI
Sbjct: 661  RTYEKRSQEELDNINYTKKLAQREMEEMQYEKLALEREREQISVRKKLLKEQEAEMHKDI 720

Query: 721  DKLNVLSKELKCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDP 780
             +L+VL   LK +R++ I +R  FL F++KLKSC +CG   + F++ DL++P D+ + D 
Sbjct: 721  TELDVLRSSLKEKRKEFICERERFLVFLEKLKSCSSCGEITENFVLSDLRLP-DVEDGDK 780

Query: 781  LANLDVESLKLFQKELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVP-PVSMKL 840
                     KL  +E       +++  R S L + + K+L++SPI +  +V    +++KL
Sbjct: 781  RFG----KQKLKAEEALNISPSAENSKRTSLLGKIASKLLSISPIGKTDKVTDLGITVKL 840

Query: 841  AADCTDLEAKEPSVSAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPD 900
                   E+ +P  S             RV+ + H  +  +    DS+ +E  EGS Q +
Sbjct: 841  P------ESSQPDDSLD-----------RVSGEDHEPSATEQSFTDSRIQEGPEGSLQSE 900

Query: 901  LKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNV 960
            +K +K +RG+G   R R  S++              G+S     V + DSK S+ ET   
Sbjct: 901  MKSDKPRRGRG-RGRGRGKSVR--------------GRSQATKAVSR-DSKPSDGETP-- 960

Query: 961  RKRPRAESSTISVSEQDGDDSERCSDSITT-GRQRKRQQKIASVQAQGESRYNLRRPKIG 1020
            RKR R ++S I+ SEQ   DS+   DSITT GR++KRQ  +   Q  G++RY LRR +  
Sbjct: 961  RKRQREQTSRITESEQAAGDSDEGVDSITTGGRRKKRQIAVPVSQTPGQTRYQLRRHRNV 1020

Query: 1021 GTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVR 1080
            GT      +    T  EK++      +  PS +   T      +GEN +      L  V 
Sbjct: 1021 GTEEDKAQASKGAT--EKQERVNDDIRKVPSPKETRTPP----EGENRENGKAEVL--VE 1080

Query: 1081 TIYRSEDRVVEFESL-------RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDD 1116
            T+   E   VE E++       +NP ++  +E          E+    E +DE+ +MI++
Sbjct: 1081 TVTHEEIVTVETETVFKVNNTGKNPVEDPQLEV-----GGSGEIREHGEEDDENISMIEE 1085

BLAST of Cp4.1LG01g16820 vs. ExPASy Swiss-Prot
Match: A0A166B1A6 (Nuclear matrix constituent protein 1 OS=Daucus carota subsp. sativus OX=79200 GN=NMCP1 PE=1 SV=2)

HSP 1 Score: 520.0 bits (1338), Expect = 6.6e-146
Identity = 403/1137 (35.44%), Postives = 634/1137 (55.76%), Query Frame = 0

Query: 55   MERKDREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQS 114
            M R +   L  K  +L++ELFDYQ+N+GL+L+EKK W SK+++L Q   ET++  K+EQ 
Sbjct: 1    MGRVEDMGLNAKLMKLETELFDYQYNMGLLLIEKKEWTSKFEELQQVYTETKDALKQEQE 60

Query: 115  AHLIALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMH 174
            AHLIA+S+ E R +NL KAL  EKQ V  L+KAL +++ + AEIK TS  KLA+A+AL+ 
Sbjct: 61   AHLIAISDAEKREENLTKALGVEKQCVLDLEKALRDMRSDYAEIKFTSDSKLAEASALIT 120

Query: 175  GIEEKSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQV 234
             +EEKSLE++ KL++A+ KLAE++RK S++E + HE+EARES L+ E+++L   +EA   
Sbjct: 121  KVEEKSLEVESKLHSADAKLAELSRKGSDIERKSHELEARESALRRERLALNAEREALTD 180

Query: 235  TSHKEKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQ 294
               ++++ LR+W++KLQE EE L+  R LLN +E++ NEN  + +QK+ +L+  +KKI+ 
Sbjct: 181  NISRQREDLREWERKLQEDEERLAEVRRLLNQREERANENDRLYQQKQSELDGEQKKIEI 240

Query: 295  SSSILKEKEDDVNRQLADVEAKEK----------------------------METQQLLH 354
                LK KEDD++ ++A +  KEK                             E Q+LL 
Sbjct: 241  IMVSLKNKEDDISSRIAKLNIKEKEADAVKHSLEVKEKDLTEFEQKLNAREQSEIQKLLD 300

Query: 355  EQSALLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKL 414
            E  A+L+ K++ FE+++++++   EN+       +++K++E+ H + KL K+E ALD+K 
Sbjct: 301  EHKAILEVKKQSFEMEMDKRKNDFENDLQNRAVEVEKKEVEVKHLEAKLAKREHALDQKH 360

Query: 415  HRTKEKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTAST 474
             + KEKE  L  KL+ L  ++K +K +E K E ER Q+L+D+  +  L  EIEK R ++ 
Sbjct: 361  EKLKEKEQYLASKLQDLNEREKSMKLEENKIEDERNQLLSDKQEMLCLKAEIEKDRASTE 420

Query: 475  QKEWQFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFER 534
            ++  +  EE E+L++ +EER E  RLQ +L QEIE+ R Q +++ KE D+LKQE+++FE+
Sbjct: 421  EQRLKLSEEIERLKITEEERLELARLQSELKQEIENCRHQRELLLKEEDELKQEKMRFEK 480

Query: 535  DWEALDEKRTEIHNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDNVKQEKE 594
            +WE LDE+RT +   L D+   ++  E L+ +EE+RL N+K     Y+Q+ELD ++  K+
Sbjct: 481  EWEDLDERRTALMKDLKDITVQKENFEKLKHSEEDRLNNKKLDTESYVQKELDALRLTKD 540

Query: 595  LFASTTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEE 654
             FA+T  HE+  L+++  ++  Q+L D EL +++LE+ L   +E++E   + RE  F+EE
Sbjct: 541  SFAATMEHEKAVLAERTSSEKKQMLNDFELWKRELETKLFNEREDMENALRLREKQFDEE 600

Query: 655  RKRERNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVL 714
            R++E N I Y+K++  KE+E++  ER ++ KEK+ + M++K L   H+ + +DI +L  L
Sbjct: 601  REKELNNINYIKEVISKEREDIKLERSRIAKEKQEILMHQKHLDEQHVVMQKDIGQLVSL 660

Query: 715  SKELKCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDV 774
            S++LK QREQ  ++R CF+ FV+  KSC+NCG    EF+V DLQ  A++     L+   +
Sbjct: 661  SEKLKDQREQFFKERECFIRFVESQKSCKNCGEMTSEFVVSDLQSLAELENLKALSVPQL 720

Query: 775  ESLKLFQ-------KEL------AASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVP 834
                L Q       K L      A       SGG  SWL++C+ KI   S  K+      
Sbjct: 721  AENYLRQDLQGTPDKNLSTVTPGAVGLGSPASGGTKSWLQKCTSKIFIFSASKKNNSPDQ 780

Query: 835  PVSMKLAADC-------TDLEAKEPSVSAG---DVKRFSFSDNIRVAEDRHAHTFDDFGN 894
              S +L  +        T++  + PS  AG   +++    S++ R  E     +  +  N
Sbjct: 781  NTSRRLHVEASPNKLLNTEVIPELPSGVAGETLEMQNMQVSNSNREMESNLNLSGTEQSN 840

Query: 895  VDSKFEEASEGSKQPDLKREKQKRGKGLNSR-HRTHSMKVTVQDAKLFLGETV------- 954
            +DSK  +  E S+Q D++   +K GK    R  R  S K   ++AK  L + +       
Sbjct: 841  IDSKALDV-EDSQQSDVRAGNRKPGKRAKGRVRRKRSAKEVAEEAKTVLADPIELNENEH 900

Query: 955  --GQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVSEQDGDDSERCSDSITTGRQR 1014
              G +       + DS    K T N RKR  ++ S  S +   G DSE  SDS+T G ++
Sbjct: 901  SNGLASAYTNESRGDSSLVGKRTRNSRKRNPSQPSQ-SAAGDVGADSEGHSDSVTAGGRQ 960

Query: 1015 KRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAF 1074
            KR++K+         RYNLRR K      A     +   G EKE D          G   
Sbjct: 961  KRRRKVVPAVQAPTGRYNLRRHKTAAPLVANGALSDPNKGKEKEID---------DGGGI 1020

Query: 1075 VTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFES--------LRNPEDNASMEKL 1116
                   VDG          L  V T+ +  + V EF S            +D  +  +L
Sbjct: 1021 GEEIPDEVDGN-------THLVQVTTLKKRINVVNEFSSAGFHGINATSESQDRDAANQL 1080

BLAST of Cp4.1LG01g16820 vs. ExPASy Swiss-Prot
Match: Q9SAF6 (Protein CROWDED NUCLEI 2 OS=Arabidopsis thaliana OX=3702 GN=CRWN2 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.2e-144
Identity = 405/1162 (34.85%), Postives = 649/1162 (55.85%), Query Frame = 0

Query: 7    KDVAFTDG------PPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 66
            K VAF+D       PPPP+G+L  +        D  +M DWR+F++ GLL+ A+ME+KD+
Sbjct: 24   KAVAFSDDLVIPTLPPPPIGTLTGQ-GVSRGHTDDMDMGDWRRFREVGLLNEASMEKKDQ 83

Query: 67   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 126
            EALLEK S L+ EL+ YQHN+GL+L+E K   SK++QL Q   E +EI KREQS+HL AL
Sbjct: 84   EALLEKISTLEKELYGYQHNMGLLLMENKELVSKHEQLNQAFQEAQEILKREQSSHLYAL 143

Query: 127  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 186
            + VE R +NL+KAL  EKQ V  L+KAL E++EE ++I+L+S+ KL +ANAL+  +  +S
Sbjct: 144  TTVEQREENLRKALGLEKQCVQELEKALREIQEENSKIRLSSEAKLVEANALVASVNGRS 203

Query: 187  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 246
             +++ K+ +AE KLAE  RKSSEL++R+ EVE RESVLQ E++S    +E+++ T  K++
Sbjct: 204  SDVENKIYSAESKLAEATRKSSELKLRLKEVETRESVLQQERLSFTKERESYEGTFQKQR 263

Query: 247  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 306
            + L +W++KLQ +EES++  +  LN +E+KVNE    +K KEK+LEE  +K+D S S  K
Sbjct: 264  EYLNEWEKKLQGKEESITEQKRNLNQREEKVNEIEKKLKLKEKELEEWNRKVDLSMSKSK 323

Query: 307  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 366
            E E+D+ ++L ++  KEK                             E Q+L+ +Q  +L
Sbjct: 324  ETEEDITKRLEELTTKEKEAHTLQITLLAKENELRAFEEKLIAREGTEIQKLIDDQKEVL 383

Query: 367  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 426
              K  EFEL+ EE R+S++ E    +  ++R+ +EI+H +EKL K+ QA++KK  R  EK
Sbjct: 384  GSKMLEFELECEEIRKSLDKELQRKIEELERQKVEIDHSEEKLEKRNQAMNKKFDRVNEK 443

Query: 427  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 486
            E DLE KLK +K ++KI++A+E++  +E+ Q+L+D++SL+ L  EIEKIR   T+KE   
Sbjct: 444  EMDLEAKLKTIKEREKIIQAEEKRLSLEKQQLLSDKESLEDLQQEIEKIRAEMTKKEEMI 503

Query: 487  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 546
             EE + L++ KEER E++RLQ +L  +IE  R+  + ++KE ++LKQE+ +FE++WE LD
Sbjct: 504  EEECKSLEIKKEEREEYLRLQSELKSQIEKSRVHEEFLSKEVENLKQEKERFEKEWEILD 563

Query: 547  EKRTEIHNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDNVKQEKELFASTT 606
            EK+   +     + E ++K E  +  E ERL+ E++ + + + +ELD+++ ++E F +  
Sbjct: 564  EKQAVYNKERIRISEEKEKFERFQLLEGERLKKEESALRVQIMQELDDIRLQRESFEANM 623

Query: 607  RHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERN 666
             HE+ AL ++ + + ++++ D+E+ R++LE  LQ+ +E+ EK   +R   FE++R  E +
Sbjct: 624  EHERSALQEKVKLEQSKVIDDLEMMRRNLEIELQERKEQDEKDLLDRMAQFEDKRMAELS 683

Query: 667  EIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKC 726
            +I + K    +E EE+ S+R  L+KE E +  ++ +L    +E+H DI +L+ LS  LK 
Sbjct: 684  DINHQKQALNREMEEMMSKRSALQKESEEIAKHKDKLKEQQVEMHNDISELSTLSINLKK 743

Query: 727  QREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPAD----IREPDPLANLDVES 786
            +RE   ++R  FLAFV KLK C +CG  + +F++ DLQ+P++    I  P  + N    S
Sbjct: 744  RREVFGRERSRFLAFVQKLKDCGSCGQLVNDFVLSDLQLPSNDEVAILPPIGVLNDLPGS 803

Query: 787  LKLFQKELAASEFDSDSGG-------RMSWLRRCSRKILNLSPIKRIGQVV---PPVSMK 846
                         D D+ G        MS L++C+  I   SP KR+   +    P    
Sbjct: 804  SNASDSCNIKKSLDGDASGSGGSRRPSMSILQKCTSII--FSPSKRVEHGIDTGKPEQRL 863

Query: 847  LAADCTDLEAKEPSVSAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQP 906
             ++    +E K       D++    S +I   ++ +          DS+ +E SEGS+  
Sbjct: 864  SSSVAVGMETKGEKPLPVDLRLRPSSSSIPEEDEEY---------TDSRVQETSEGSQLS 923

Query: 907  DLKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTN 966
            + +  ++ RG+               + AK  L  T            S  K ++ E ++
Sbjct: 924  EFQSSRRGRGR--------------PRKAKPALNPT------------SSVKHASLEESS 983

Query: 967  VRKRPRAESSTISVSEQDGDDSERCSDSITTGRQRKRQQKIASV-QAQGESRYNLRRPKI 1026
              +     S T   +   G    +  D   TG +R+RQQ +A + Q  G+  YNLRR K 
Sbjct: 984  KDELSGHVSVTSKKTTGGGGRKRQHIDDTATGGKRRRQQTVAVLPQTPGQRHYNLRRKKT 1043

Query: 1027 GGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTV 1086
                 A +V  N   G E + D   +A  + + E  V  +LR+   E    AD V     
Sbjct: 1044 VDQVPA-DVEDNAAAG-EDDADIAASAPSKDTVEETVVETLRARRIET--NADVVSAEN- 1103

Query: 1087 RTIYRSEDRVVEFESLRNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYD 1116
                  +  V   E   N + N             DE  + ++ +D + N  DD +D+ D
Sbjct: 1104 ----NGDVPVANVEPTVNEDTNED----------GDEEEDEAQDDDNEENQDDDDDDDGD 1128

BLAST of Cp4.1LG01g16820 vs. ExPASy Swiss-Prot
Match: I0J0E7 (Nuclear matrix constituent protein 1 OS=Allium cepa OX=4679 GN=NMCP1 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.3e-137
Identity = 399/1194 (33.42%), Postives = 661/1194 (55.36%), Query Frame = 0

Query: 15   PPPPLGSLNDRFHKIAAAADTG--NMDDWRKFKKAGLLDAAAMERKDREALLEKASRLQS 74
            P PPLG LN          D G  +M+ W++FK  GLLD +   +KDRE+L  +   L+ 
Sbjct: 37   PFPPLGLLN------GGDLDRGGEDMEAWKRFKDEGLLDESICYKKDRESLASRIIELEK 96

Query: 75   ELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIALSEVETRRDNLKK 134
            +L +YQ+N+GL+L+EKK W+S ++++   LAE EEI KREQ+AH+IAL+E E R DNL+K
Sbjct: 97   DLHEYQYNMGLLLIEKKEWSSHFEEMKMRLAEAEEILKREQAAHIIALTESEKREDNLRK 156

Query: 135  ALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKSLELQKKLNAAEV 194
            AL  EKQ V+ L+KAL E++ E AE+K T++KK+ +A AL   IEEK L+ ++KL++A+ 
Sbjct: 157  ALGVEKQCVTDLEKALREMRSEIAEVKYTAEKKMTEAFALEASIEEKRLDTERKLHSADA 216

Query: 195  KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEKDGLRKWQQKLQE 254
            KLAE +RKSSE+  ++ +VE RE  +Q E  S+ + ++A +    ++K+ LR+W++KLQ+
Sbjct: 217  KLAEASRKSSEINRKLEDVEDRERKVQRELNSINSERKALEKDISEQKEHLREWEKKLQD 276

Query: 255  REESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILKEKEDDVNRQLAD 314
             +  L   +  +N++E+++NE    +K+KE++LEE K+ I+ + + LK KE+D++ +L  
Sbjct: 277  GQNRLLDGQRHINEREERINEAEGGLKKKEEELEEAKRSIEGTRNTLKRKEEDLDVRLRS 336

Query: 315  VEAKEK----------------------------METQQLLHEQSALLQKKREEFELQLE 374
            + +KEK                             E Q+LL E  A L  K+ EFEL+LE
Sbjct: 337  LVSKEKEIELKMKNLQKKEKDLHEIAEKLDHREREEIQKLLDEHRATLDTKKREFELELE 396

Query: 375  EKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEKEGDLERKLKALK 434
             KR+SV+ E      A+ + + E+N K+  + + E+ L+ K+ + K KE DLE K KALK
Sbjct: 397  SKRKSVDEELKSKFAAVNKAEKEVNRKQGLISEGEKELESKMDKIKIKEKDLETKSKALK 456

Query: 435  AKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQFHEEREKLQVIKE 494
              ++ LK+DE+K   E+ Q++ D   L+  I+E+E +R A   ++ Q  EEREKL++ KE
Sbjct: 457  KWEESLKSDEKKLVAEKDQIMKDTHELKVSINELESLRDALNAEQHQIAEEREKLEISKE 516

Query: 495  ERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALDEKRTEIHNGLGD 554
            ER ++++ Q +L QEIE YR   + ++K  + L++ER KFE++WE+LDEK+  +      
Sbjct: 517  EREQYIQKQSELKQEIEKYRNMQEELSKGIESLREEREKFEKEWESLDEKKITLQRETKK 576

Query: 555  LEELRKKLEIL-RTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTRHEQQALSQQAQ 614
            + E ++KLE     ++ERLRNE+      ++R+L+++K +KE F +T +HE+    ++  
Sbjct: 577  IHEEKEKLEKWHHKDQERLRNEEANAKADIERQLEDIKLQKEAFENTMKHERLMAQEEVA 636

Query: 615  NKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNEIFYLKDIALKE 674
             +   + +++EL++ DLE ++QK QEE+E++ Q +E  FE  ++ E + I  L ++   +
Sbjct: 637  RRLADVTRELELRKHDLEMNMQKKQEEIERKLQGKEREFETRKEAELSRITSLINLNNSK 696

Query: 675  KEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQREQLIQDRICF 734
             +++  E+ +L++EKE V + +K+L  D  EI +D+D L  LSK LK QR + I+++ CF
Sbjct: 697  LQKLRIEQDRLDREKEEVELQKKKLQEDQSEIQRDVDTLRQLSKNLKNQRAEFIKEKECF 756

Query: 735  LAFVDKLKSCENCGVSIKEF-MVPDLQMPADIREPD-PLANLDVESLKLFQKELAASEFD 794
            LA  ++ K+C+NCGVSI E  MV  +Q  A+I   D  L +L  + ++   K   +    
Sbjct: 757  LAAAERCKTCQNCGVSISELEMVGIIQSSAEIENADIVLPSLTDDHIEQHMKNKGSHVTS 816

Query: 795  SDSGGRM---SWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSVS----- 854
              +G R+    +L++C+ KI   SP K   +     +  +  +  D+ A E + +     
Sbjct: 817  PQTGSRVFGSGFLQKCT-KIFKFSPGKN-AETSATTTPLVFGEELDIAASEDAAANDNNP 876

Query: 855  AGDVKRFS------FSDNIRVAEDRHAHTFDD---------------FGNVDSKFEEASE 914
            A DV+R +      F + +  A    A   D+                  V ++  E  E
Sbjct: 877  AADVERVTVNPSLVFGEQLDTAASEDAAANDNNPAADVERVTVNPPPLAPVATEQNETEE 936

Query: 915  GSKQPDLK--------REKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQ 974
             S  P+          R+  +RG+G  +  RT +M+  V DAK  LG+T+   +     Q
Sbjct: 937  SSLPPENDSPPKQRGGRQSTRRGRGGKTVRRTRTMEAVVDDAKAILGDTLIVEEAKESSQ 996

Query: 975  KSDSKFSNKE-----TTNVR-KRPRAESSTISVSEQDGDDSERCSDSITTGR-QRKRQQK 1034
            ++D +          T+N R KR RA +S ++ SE D ++SE  S SI+ GR +RK++Q 
Sbjct: 997  QNDEQSQGASVHTGGTSNTRQKRRRAPASEMTNSEHDVEESESQSQSISIGRGRRKKRQT 1056

Query: 1035 IASVQAQG---ESRYNLRRPKIGGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVT 1094
             A+ + Q    E RYNLR      T +   V+  L    + +     + +          
Sbjct: 1057 SAASEVQAPVVERRYNLRH----STVAKNSVAATLAVSDQAKVQTKASHQASHDNNQISM 1116

Query: 1095 SSLRSVDGENIKKADYVQLTTVRTIY---------RSEDRVVEFESLRNPEDNASME--- 1116
                +++G + K    VQ TT  ++           + +  +   S+   E +AS E   
Sbjct: 1117 GDDPALEGSH-KVTHTVQKTTTASVMEVSSKPAMEETHEENIVVRSVEISEMSASEEAEG 1176

BLAST of Cp4.1LG01g16820 vs. NCBI nr
Match: XP_023530139.1 (protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023530145.1 protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2027 bits (5251), Expect = 0.0
Identity = 1115/1143 (97.55%), Postives = 1115/1143 (97.55%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. NCBI nr
Match: XP_022930031.1 (protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita moschata] >XP_022930032.1 protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2001 bits (5183), Expect = 0.0
Identity = 1101/1143 (96.33%), Postives = 1107/1143 (96.85%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            L+LQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISL TRKEAHQVTSHKE+
Sbjct: 181  LKLQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLATRKEAHQVTSHKEE 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR SVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRHSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            I YLKDIALKEKEE+SSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ
Sbjct: 661  ILYLKDIALKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPD LANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDLLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLV MDDLCDEVN+TSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVGMDDLCDEVNDTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. NCBI nr
Match: KAG6601702.1 (Protein CROWDED NUCLEI 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1995 bits (5169), Expect = 0.0
Identity = 1098/1143 (96.06%), Postives = 1105/1143 (96.68%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRD LQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDRLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKT+MLIYMQ ELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTEMLIYMQGELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRE+NE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKREQNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLKDIA KEKEE+SSERHQLEKEKEVVTMNRKELIADHLEI QDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKDIAQKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIRQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKL ADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLPADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCS+SITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSESITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRS DGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSADGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLV MDDLCDEVN+TSEYEDEDGN+IDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVGMDDLCDEVNDTSEYEDEDGNIIDDAEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. NCBI nr
Match: XP_022993965.1 (protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita maxima] >XP_022993975.1 protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita maxima] >XP_022993983.1 protein CROWDED NUCLEI 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1977 bits (5123), Expect = 0.0
Identity = 1088/1143 (95.19%), Postives = 1101/1143 (96.33%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKK+WASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKVWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            D LRKWQQKL EREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DSLRKWQQKLHEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKED+VNRQLADVEAKEK                            METQQLLHEQSA+L
Sbjct: 301  EKEDEVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSAIL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR+SVENEGSI LGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRKSVENEGSIRLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDK LKADERKHEVERLQMLADRDSLQSLID+IEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKTLKADERKHEVERLQMLADRDSLQSLIDKIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEE RKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEEERKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLK+IA KEKEE+SSERHQLEKEKEVVTMNRKELIADHLEI QDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKNIAQKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIRQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            +ELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  EELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSF DNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFRDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKET NVRKRPR ESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETINVRKRPRVESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCS+SITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSESITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLVAMDDLCDEVN+TSEYEDEDGN+IDD EDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVAMDDLCDEVNDTSEYEDEDGNIIDDVEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. NCBI nr
Match: XP_023530151.1 (protein CROWDED NUCLEI 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1864 bits (4829), Expect = 0.0
Identity = 1045/1143 (91.43%), Postives = 1045/1143 (91.43%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSEL                                              
Sbjct: 61   EALLEKASRLQSEL---------------------------------------------- 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
                                    KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  ------------------------KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1073

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1073

BLAST of Cp4.1LG01g16820 vs. ExPASy TrEMBL
Match: A0A6J1EPS4 (protein CROWDED NUCLEI 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436467 PE=3 SV=1)

HSP 1 Score: 2001 bits (5183), Expect = 0.0
Identity = 1101/1143 (96.33%), Postives = 1107/1143 (96.85%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            L+LQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISL TRKEAHQVTSHKE+
Sbjct: 181  LKLQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLATRKEAHQVTSHKEE 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR SVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRHSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            I YLKDIALKEKEE+SSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ
Sbjct: 661  ILYLKDIALKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPD LANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDLLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLV MDDLCDEVN+TSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVGMDDLCDEVNDTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. ExPASy TrEMBL
Match: A0A6J1K004 (protein CROWDED NUCLEI 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489810 PE=3 SV=1)

HSP 1 Score: 1977 bits (5123), Expect = 0.0
Identity = 1088/1143 (95.19%), Postives = 1101/1143 (96.33%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSELFDYQHNLGLILLEKK+WASKYDQLGQDLAETEEIFKREQSAHLIAL
Sbjct: 61   EALLEKASRLQSELFDYQHNLGLILLEKKVWASKYDQLGQDLAETEEIFKREQSAHLIAL 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
            SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            D LRKWQQKL EREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DSLRKWQQKLHEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKED+VNRQLADVEAKEK                            METQQLLHEQSA+L
Sbjct: 301  EKEDEVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSAIL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR+SVENEGSI LGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRKSVENEGSIRLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDK LKADERKHEVERLQMLADRDSLQSLID+IEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKTLKADERKHEVERLQMLADRDSLQSLIDKIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEE RKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEEERKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLK+IA KEKEE+SSERHQLEKEKEVVTMNRKELIADHLEI QDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKNIAQKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIRQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            +ELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  EELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSF DNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFRDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKET NVRKRPR ESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETINVRKRPRVESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCS+SITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSESITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1080

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLVAMDDLCDEVN+TSEYEDEDGN+IDD EDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVAMDDLCDEVNDTSEYEDEDGNIIDDVEDEYDEEQPDAKSIGKKIWTF 1140

BLAST of Cp4.1LG01g16820 vs. ExPASy TrEMBL
Match: A0A6J1ETW4 (protein CROWDED NUCLEI 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436467 PE=3 SV=1)

HSP 1 Score: 1838 bits (4761), Expect = 0.0
Identity = 1031/1143 (90.20%), Postives = 1037/1143 (90.73%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSEL                                              
Sbjct: 61   EALLEKASRLQSEL---------------------------------------------- 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
                                    KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  ------------------------KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            L+LQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISL TRKEAHQVTSHKE+
Sbjct: 181  LKLQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLATRKEAHQVTSHKEE 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKEDDVNRQLADVEAKEK                            METQQLLHEQSALL
Sbjct: 301  EKEDDVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSALL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR SVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRHSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            I YLKDIALKEKEE+SSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ
Sbjct: 661  ILYLKDIALKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPD LANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDLLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1073

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLV MDDLCDEVN+TSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVGMDDLCDEVNDTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1073

BLAST of Cp4.1LG01g16820 vs. ExPASy TrEMBL
Match: A0A6J1JUG6 (protein CROWDED NUCLEI 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489810 PE=3 SV=1)

HSP 1 Score: 1816 bits (4705), Expect = 0.0
Identity = 1019/1143 (89.15%), Postives = 1031/1143 (90.20%), Query Frame = 0

Query: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60
            MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR
Sbjct: 1    MFTPQRKDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 60

Query: 61   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 120
            EALLEKASRLQSEL                                              
Sbjct: 61   EALLEKASRLQSEL---------------------------------------------- 120

Query: 121  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180
                                    KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS
Sbjct: 121  ------------------------KKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 180

Query: 181  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240
            LELQKKLNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK
Sbjct: 181  LELQKKLNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 240

Query: 241  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300
            D LRKWQQKL EREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK
Sbjct: 241  DSLRKWQQKLHEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 300

Query: 301  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 360
            EKED+VNRQLADVEAKEK                            METQQLLHEQSA+L
Sbjct: 301  EKEDEVNRQLADVEAKEKEADLSRSLLEKKQEELDHMEENLRGRERMETQQLLHEQSAIL 360

Query: 361  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420
            QKKREEFELQLEEKR+SVENEGSI LGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK
Sbjct: 361  QKKREEFELQLEEKRKSVENEGSIRLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 420

Query: 421  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 480
            EGDLERKLKALKAKDK LKADERKHEVERLQMLADRDSLQSLID+IEKIRTASTQKEWQF
Sbjct: 421  EGDLERKLKALKAKDKTLKADERKHEVERLQMLADRDSLQSLIDKIEKIRTASTQKEWQF 480

Query: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540
            HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD
Sbjct: 481  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 540

Query: 541  EKRTEIHNGLGDLEELRKKLEILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTR 600
            EKRTEIHNGLGDLEE RKKLEILRTEEERLRNEKT+MLIYMQRELDNVKQEKELFASTTR
Sbjct: 541  EKRTEIHNGLGDLEEERKKLEILRTEEERLRNEKTEMLIYMQRELDNVKQEKELFASTTR 600

Query: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660
            HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE
Sbjct: 601  HEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNE 660

Query: 661  IFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQ 720
            IFYLK+IA KEKEE+SSERHQLEKEKEVVTMNRKELIADHLEI QDIDKLNVLSKELKCQ
Sbjct: 661  IFYLKNIAQKEKEELSSERHQLEKEKEVVTMNRKELIADHLEIRQDIDKLNVLSKELKCQ 720

Query: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780
            REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ
Sbjct: 721  REQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQ 780

Query: 781  KELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840
            +ELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV
Sbjct: 781  EELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSV 840

Query: 841  SAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNS 900
            SAGDVKRFSF DNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKR KGLNS
Sbjct: 841  SAGDVKRFSFRDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKREKGLNS 900

Query: 901  RHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNVRKRPRAESSTISVS 960
            RHRTHSMK TVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKET NVRKRPR ESSTISVS
Sbjct: 901  RHRTHSMKATVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETINVRKRPRVESSTISVS 960

Query: 961  EQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020
            EQDGDDSERCS+SITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG
Sbjct: 961  EQDGDDSERCSESITTGRQRKRQQKIASVQAQGESRYNLRRPKIGGTASATEVSGNLTTG 1020

Query: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESL 1080
            MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVV+FESL
Sbjct: 1021 MEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVRTIYRSEDRVVQFESL 1073

Query: 1081 RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYDEEQPDAKSIGKKIWTF 1115
            RNPEDNASMEKLVAMDDLCDEVN+TSEYEDEDGN+IDD EDEYDEEQPDAKSIGKKIWTF
Sbjct: 1081 RNPEDNASMEKLVAMDDLCDEVNDTSEYEDEDGNIIDDVEDEYDEEQPDAKSIGKKIWTF 1073

BLAST of Cp4.1LG01g16820 vs. ExPASy TrEMBL
Match: A0A1S3CSZ3 (protein CROWDED NUCLEI 3 OS=Cucumis melo OX=3656 GN=LOC103504607 PE=3 SV=1)

HSP 1 Score: 1501 bits (3885), Expect = 0.0
Identity = 866/1163 (74.46%), Postives = 963/1163 (82.80%), Query Frame = 0

Query: 7    KDVAFTDGPPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDREALLEK 66
            KDV FTD PPPPLGSLND  +K A A DTG+MDDWRKFKKAGLLDAAAMERKDREALLEK
Sbjct: 34   KDVIFTDDPPPPLGSLNDELYKTATAVDTGDMDDWRKFKKAGLLDAAAMERKDREALLEK 93

Query: 67   ASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIALSEVETR 126
            ASRLQSELFDYQHN+GL+L+EKK WA K+DQL QDLAETEEIFKREQSAHLIALSEVETR
Sbjct: 94   ASRLQSELFDYQHNMGLLLIEKKDWALKFDQLEQDLAETEEIFKREQSAHLIALSEVETR 153

Query: 127  RDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKSLELQKK 186
            RDNLKKALAAEKQHVSSLKK+L EV EERAEIKLTSQKKLADANALMHGIEEKSLELQKK
Sbjct: 154  RDNLKKALAAEKQHVSSLKKSLYEVNEERAEIKLTSQKKLADANALMHGIEEKSLELQKK 213

Query: 187  LNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEKDGLRKW 246
            LNAAE KLAEVNRKSSELEMRMHEVEARESVLQTEQISLVT KEAH+ TSHKE++ LRKW
Sbjct: 214  LNAAEAKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTGKEAHEATSHKERESLRKW 273

Query: 247  QQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILKEKEDDV 306
            QQKLQEREE LS+SRELLNDKEQKV+EN T MKQKEK+LEE+KKKID SSS+LK +ED+V
Sbjct: 274  QQKLQEREEKLSKSRELLNDKEQKVSENSTTMKQKEKNLEEMKKKIDLSSSVLKGREDNV 333

Query: 307  NRQLADVEAKEK----------------------------METQQLLHEQSALLQKKREE 366
            NR+LADVEAKEK                            ME QQLL EQS +LQKK+E+
Sbjct: 334  NRRLADVEAKEKEADFSRSLLEKKQEELHQMEENLHGREMMEIQQLLDEQSVILQKKKEQ 393

Query: 367  FELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEKEGDLER 426
            FELQLEEKRQS++NEG   LG +KRKD+EINH+KEKLVKQEQALDKKL R KEKEGDLE+
Sbjct: 394  FELQLEEKRQSLDNEGITKLGTLKRKDLEINHEKEKLVKQEQALDKKLLRAKEKEGDLEQ 453

Query: 427  KLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQFHEEREK 486
            K+K LK+KD+ILKADE+K EVERLQMLADR+SL+SLI+EIE+IRT ++QKE QFHEER+K
Sbjct: 454  KIKTLKSKDRILKADEKKLEVERLQMLADRESLRSLINEIEEIRTENSQKEQQFHEERDK 513

Query: 487  LQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALDEKRTEI 546
            LQV+KEERSEHVRLQCQLMQEIESYRLQNKIV KEH+DLKQERVKFERDWEAL+EKRTEI
Sbjct: 514  LQVMKEERSEHVRLQCQLMQEIESYRLQNKIVMKEHEDLKQERVKFERDWEALNEKRTEI 573

Query: 547  HNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDNVKQEKELFASTTRHEQQA 606
            H+ L DL E R KLEIL+  EEERLRNEK +MLIYMQREL+NVKQEKELFASTTR EQQ+
Sbjct: 574  HDELSDLVEERTKLEILQEAEEERLRNEKNEMLIYMQRELENVKQEKELFASTTRQEQQS 633

Query: 607  LSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERNEIFYLK 666
            LSQQAQ +H+QLLQDIE QRKDLESH Q SQ +LEK RQERELAFEEE++RERN++F L+
Sbjct: 634  LSQQAQTEHSQLLQDIEFQRKDLESHFQNSQMKLEKVRQERELAFEEEKERERNKLFCLR 693

Query: 667  DIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKCQREQLI 726
            DIA KE +E+ SERHQLEKEKEVV++NRK++IADHLEIHQDIDKLN+LSKELK QREQLI
Sbjct: 694  DIAQKETDELLSERHQLEKEKEVVSLNRKQMIADHLEIHQDIDKLNILSKELKIQREQLI 753

Query: 727  QDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDPLANLDVESLKLFQKELAA 786
            +DR+ FL FVDK KSC  CGVSI+EF+VPDLQ+P +IR+  PL  L+  SL+  Q+E AA
Sbjct: 754  RDRVRFLTFVDKHKSCGKCGVSIEEFIVPDLQIPEEIRKSHPLPMLEANSLQNLQREFAA 813

Query: 787  SEF-DSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLAADCTDLEAKEPSVSAGD 846
            SEF  SDSGGRMSWLRRCSRKILNLSPIK+IG VVP V MKLAADCTDLE KEP V+ GD
Sbjct: 814  SEFGSSDSGGRMSWLRRCSRKILNLSPIKKIGHVVPSVPMKLAADCTDLEVKEPRVNVGD 873

Query: 847  VKR----------------------FSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGS 906
            VKR                      FSFSDNIR+AE+ H HT DDF N+DSKFEEASE S
Sbjct: 874  VKRSGIAYEPQQSSFIESEPSDVQRFSFSDNIRLAENTHEHTLDDFNNLDSKFEEASEAS 933

Query: 907  KQPDLKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKE 966
            KQPD+KRE+ K  KGL S HRT S+K TVQDAK+FLGET GQSDLNV VQ SDS    KE
Sbjct: 934  KQPDMKRERPKHEKGLKSGHRTRSVKATVQDAKVFLGETAGQSDLNVPVQ-SDSNSLYKE 993

Query: 967  TTNVRKRPRAESSTISVSEQDGDDSERCSDSITTGRQRKRQQKIASVQAQGESRYNLRRP 1026
            T+NVRKRP  ESS +S+SEQDG+DSE CSDSITT RQRKR+QKI SVQ QGESRYNLRR 
Sbjct: 994  TSNVRKRPLPESSAVSISEQDGNDSEGCSDSITTARQRKRRQKIPSVQTQGESRYNLRRH 1053

Query: 1027 KIGGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLT 1086
            K  G ASAT+VS NLTT MEKE + T                  +V GEN +K D V++T
Sbjct: 1054 KNAGKASATQVSPNLTTVMEKENEETTP----------------TVGGENGEKMDSVKIT 1113

Query: 1087 TVRTIYRSEDRVVEFESLRNPEDNASMEKLVA--MDDLCDEVNNTSEYEDEDGNMIDDAE 1115
            TVRTIY SEDRVV FES R  EDNA MEKLV   ++DLC E N +SEYEDED +++DD E
Sbjct: 1114 TVRTIYHSEDRVVRFESQRTAEDNAPMEKLVTTTVNDLCHEANGSSEYEDEDQSILDD-E 1173

BLAST of Cp4.1LG01g16820 vs. TAIR 10
Match: AT1G67230.1 (little nuclei1 )

HSP 1 Score: 547.0 bits (1408), Expect = 3.6e-155
Identity = 412/1119 (36.82%), Postives = 654/1119 (58.45%), Query Frame = 0

Query: 59   DREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLI 118
            D   L EK S L+ ELF+YQH++GL+L+EKK W+S+Y+ L Q   E  E  K+E++AHLI
Sbjct: 48   DPRILPEKISELEKELFEYQHSMGLLLIEKKEWSSQYEALQQAFEEVNECLKQERNAHLI 107

Query: 119  ALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEE 178
            A+++VE R + L+KAL  EKQ    L+KAL E++ E AEIK T+  KL +ANAL+  +EE
Sbjct: 108  AIADVEKREEGLRKALGIEKQCALDLEKALKELRAENAEIKFTADSKLTEANALVRSVEE 167

Query: 179  KSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHK 238
            KSLE++ KL A + KLAEV+RKSS++E +  EVEARES LQ E+ S +  +EA + T  K
Sbjct: 168  KSLEVEAKLRAVDAKLAEVSRKSSDVERKAKEVEARESSLQRERFSYIAEREADEATLSK 227

Query: 239  EKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSI 298
            +++ LR+W++KLQE EE +++S+ ++  +E + NE+  I+KQK K+LEE +KKID ++  
Sbjct: 228  QREDLREWERKLQEGEERVAKSQMIVKQREDRANESDKIIKQKGKELEEAQKKIDAANLA 287

Query: 299  LKEKEDDVNRQLAD----------------------------VEAKEKMETQQLLHEQSA 358
            +K+ EDDV+ ++ D                            +EA+EKM  QQL+ E  A
Sbjct: 288  VKKLEDDVSSRIKDLALREQETDVLKKSIETKARELQALQEKLEAREKMAVQQLVDEHQA 347

Query: 359  LLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTK 418
             L   + EFEL++E+KR+S+++     +  +++++ E  H +EK+ K+EQALD+KL + K
Sbjct: 348  KLDSTQREFELEMEQKRKSIDDSLKSKVAEVEKREAEWKHMEEKVAKREQALDRKLEKHK 407

Query: 419  EKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEW 478
            EKE D + +LK +  ++K LK++E+  E E+ ++L D++ + +L   +EK+   +  +  
Sbjct: 408  EKENDFDLRLKGISGREKALKSEEKALETEKKKLLEDKEIILNLKALVEKVSGENQAQLS 467

Query: 479  QFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEA 538
            + ++E+++L+V +EERSE++RLQ +L ++IE  R Q +++ KE +DLK +R  FE++WE 
Sbjct: 468  EINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQELLQKEAEDLKAQRESFEKEWEE 527

Query: 539  LDEKRTEIHNGLGDLEELRKKLE-ILRTEEERLRNEKTKMLIYMQRELDNVKQEKELFAS 598
            LDE++ +I N L ++ + ++KLE  +  EEERL+ EK      M+REL+ ++  K  FA 
Sbjct: 528  LDERKAKIGNELKNITDQKEKLERHIHLEEERLKKEKQAANENMERELETLEVAKASFAE 587

Query: 599  TTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRE 658
            T  +E+  LS++A+++ +QLL DIE++++ LES +Q   EE E+  Q ++  FEEER++E
Sbjct: 588  TMEYERSMLSKKAESERSQLLHDIEMRKRKLESDMQTILEEKERELQAKKKLFEEEREKE 647

Query: 659  RNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKEL 718
             + I YL+D+A +E  ++ +ER ++EKEK  V  ++  L     EI +D+D L  L+K+L
Sbjct: 648  LSNINYLRDVARREMMDMQNERQRIEKEKLEVDSSKNHLEEQQTEIRKDVDDLVALTKKL 707

Query: 719  KCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPD---LQMPADIREPDPLAN-LDV 778
            K QREQ I +R  FL+ ++  ++C  CG  + E ++P+   L+MP        LAN LD 
Sbjct: 708  KEQREQFISERSRFLSSMESNRNCSRCGELLSELVLPEIDNLEMP----NMSKLANILDN 767

Query: 779  ESLKLFQKEL---AASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVPPVSMKLA--- 838
            E+ +   +++   AA      +GG++SW R+C+ K+L LSPIK      P V+  LA   
Sbjct: 768  EAPRQEMRDISPTAAGLGLPVTGGKVSWFRKCTSKMLKLSPIK---MTEPSVTWNLADQE 827

Query: 839  ---ADCTDLEAKEPSVSAG-----DVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEE-A 898
                +  ++     +V A      DV++       +  E  + ++  D  +++SK +E A
Sbjct: 828  PQSTEQANVGGPSTTVQAATTYSFDVQKAESETGTKEVEVTNVNSDGDQSDINSKAQEVA 887

Query: 899  SEGSKQPDLKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSD--S 958
            ++     D+  + + +GKG     RT S+K  V DAK   GE++   + N   +  D  +
Sbjct: 888  ADSLSNLDVDGQSRMKGKGKARTRRTRSVKDVVDDAKALYGESINLYEPNDSTENVDDST 947

Query: 959  KFSNKET--------TNVRKRPRAESSTISVSEQDGDDSERCSDSITTG-RQRKRQQKIA 1018
            K S  ET         N RKR R  S     +EQDG++S+  SDS+T G  QRKR+QK+A
Sbjct: 948  KASTGETGRSDKAISKNGRKRGRVGSLRTCTTEQDGNESDGKSDSVTGGAHQRKRRQKVA 1007

Query: 1019 SVQAQGE---SRYNLRRPKIGGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSS 1078
            S Q QGE    RYNLRRP+      A         G+++E+    T       +A  T+S
Sbjct: 1008 SEQ-QGEVVGQRYNLRRPRRVTGEPALSKKNEDIGGVQQEEGIHCT-------QATATAS 1067

Query: 1079 LRSVDGENIKKADYVQLTTVRTIYRSEDRVVEFESLRNPEDNASMEKLVAMDDLCDEVNN 1116
            +     +N    + VQ         SED            D  S ++    + + ++VN 
Sbjct: 1068 VGVAVSDNGVSTNVVQHEATAD---SED-----------TDAGSPKRTDESEAMSEDVNK 1127

BLAST of Cp4.1LG01g16820 vs. TAIR 10
Match: AT1G68790.1 (little nuclei3 )

HSP 1 Score: 520.4 bits (1339), Expect = 3.6e-147
Identity = 416/1169 (35.59%), Postives = 661/1169 (56.54%), Query Frame = 0

Query: 1    MFTPQR----------KDVAFTDG--PPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAG 60
            MFTPQR          K +AF+D    PPP         ++    D    DDW+KFK+ G
Sbjct: 1    MFTPQRNRWPETDRKGKAIAFSDEIITPPP--------QRVLLRED----DDWQKFKEVG 60

Query: 61   LLDAAAMERKDREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEI 120
            LLD A++ERKDR+AL+EK  +L+ ELFDYQHN+GL+L+EKK W S  ++L Q   E  E+
Sbjct: 61   LLDEASLERKDRDALIEKILKLEKELFDYQHNMGLLLIEKKQWTSTNNELQQAYDEAMEM 120

Query: 121  FKREQSAHLIALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLAD 180
             KRE++++ I L+E + R +NL+KAL  EKQ V+ L+  L   + E + +K TS+ KL +
Sbjct: 121  LKREKTSNAITLNEADKREENLRKALIDEKQFVAELENDLKYWQREHSVVKSTSEAKLEE 180

Query: 181  ANALMHGIEEKSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTR 240
            ANAL+ G++EK+LE+ ++   AE K + +NRKSSELE ++ EVE RE V Q E +SLVT 
Sbjct: 181  ANALVIGMKEKALEVDRERAIAEEKFSVMNRKSSELERKLKEVETREKVHQREHLSLVTE 240

Query: 241  KEAHQVTSHKEKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEI 300
            +EAH+   +K+++ L++W++KL   E+ LS  +  +N +E++V EN   +++KEK LE +
Sbjct: 241  REAHEAVFYKQREDLQEWEKKLTLEEDRLSEVKRSINHREERVMENERTIEKKEKILENL 300

Query: 301  KKKIDQSSSILKEKEDDVNRQLADVEAKEK----------------------------ME 360
            ++KI  + S L EKE+ +  +L D+  KEK                            ME
Sbjct: 301  QQKISVAKSELTEKEESIKIKLNDISLKEKDFEAMKAKVDIKEKELHEFEENLIEREQME 360

Query: 361  TQQLLHEQSALLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQ 420
              +LL +Q A+L  +R EFE++LE+ R+S++ E       I++  +EI+HK+EKL K+E 
Sbjct: 361  IGKLLDDQKAVLDSRRREFEMELEQMRRSLDEELEGKKAEIEQLQVEISHKEEKLAKREA 420

Query: 421  ALDKKLHRTKEKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEK 480
            AL+KK    K+KE DL+ +LK +K K+K LKA+E+K  +E  ++L D++ L+ L DEIE+
Sbjct: 421  ALEKKEEGVKKKEKDLDARLKTVKEKEKALKAEEKKLHMENERLLEDKECLRKLKDEIEE 480

Query: 481  IRTASTQKEWQFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQE 540
            I T +T++E +  EE E L++ KEER E +RLQ +L Q+I+  + + +++ KE ++LKQ+
Sbjct: 481  IGTETTKQESRIREEHESLRITKEERVEFLRLQSELKQQIDKVKQEEELLLKEREELKQD 540

Query: 541  RVKFERDWEALDEKRTEIHNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDN 600
            + +FE++WEALD+KR  I     ++ E  +KL  L+ +E+ RL+ E+      ++RELD 
Sbjct: 541  KERFEKEWEALDKKRANITREQNEVAEENEKLRNLQISEKHRLKREEMTSRDNLKRELDG 600

Query: 601  VKQEKELFASTTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERE 660
            VK +KE F +                    ++D+E+Q+++L+   Q+ +E  E+   ER 
Sbjct: 601  VKMQKESFEAD-------------------MEDLEMQKRNLDMEFQRQEEAGERDFNERA 660

Query: 661  LAFEEERKRERNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDI 720
              +E+  + E + I Y K +A +E EE+  E+  LE+E+E +++ +K L     E+H+DI
Sbjct: 661  RTYEKRSQEELDNINYTKKLAQREMEEMQYEKLALEREREQISVRKKLLKEQEAEMHKDI 720

Query: 721  DKLNVLSKELKCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPADIREPDP 780
             +L+VL   LK +R++ I +R  FL F++KLKSC +CG   + F++ DL++P D+ + D 
Sbjct: 721  TELDVLRSSLKEKRKEFICERERFLVFLEKLKSCSSCGEITENFVLSDLRLP-DVEDGDK 780

Query: 781  LANLDVESLKLFQKELAASEFDSDSGGRMSWLRRCSRKILNLSPIKRIGQVVP-PVSMKL 840
                     KL  +E       +++  R S L + + K+L++SPI +  +V    +++KL
Sbjct: 781  RFG----KQKLKAEEALNISPSAENSKRTSLLGKIASKLLSISPIGKTDKVTDLGITVKL 840

Query: 841  AADCTDLEAKEPSVSAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPD 900
                   E+ +P  S             RV+ + H  +  +    DS+ +E  EGS Q +
Sbjct: 841  P------ESSQPDDSLD-----------RVSGEDHEPSATEQSFTDSRIQEGPEGSLQSE 900

Query: 901  LKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTNV 960
            +K +K +RG+G   R R  S++              G+S     V + DSK S+ ET   
Sbjct: 901  MKSDKPRRGRG-RGRGRGKSVR--------------GRSQATKAVSR-DSKPSDGETP-- 960

Query: 961  RKRPRAESSTISVSEQDGDDSERCSDSITT-GRQRKRQQKIASVQAQGESRYNLRRPKIG 1020
            RKR R ++S I+ SEQ   DS+   DSITT GR++KRQ  +   Q  G++RY LRR +  
Sbjct: 961  RKRQREQTSRITESEQAAGDSDEGVDSITTGGRRKKRQIAVPVSQTPGQTRYQLRRHRNV 1020

Query: 1021 GTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTVR 1080
            GT      +    T  EK++      +  PS +   T      +GEN +      L  V 
Sbjct: 1021 GTEEDKAQASKGAT--EKQERVNDDIRKVPSPKETRTPP----EGENRENGKAEVL--VE 1080

Query: 1081 TIYRSEDRVVEFESL-------RNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDD 1116
            T+   E   VE E++       +NP ++  +E          E+    E +DE+ +MI++
Sbjct: 1081 TVTHEEIVTVETETVFKVNNTGKNPVEDPQLEV-----GGSGEIREHGEEDDENISMIEE 1085

BLAST of Cp4.1LG01g16820 vs. TAIR 10
Match: AT1G13220.2 (nuclear matrix constituent protein-related )

HSP 1 Score: 513.5 bits (1321), Expect = 4.4e-145
Identity = 405/1162 (34.85%), Postives = 649/1162 (55.85%), Query Frame = 0

Query: 7    KDVAFTDG------PPPPLGSLNDRFHKIAAAADTGNMDDWRKFKKAGLLDAAAMERKDR 66
            K VAF+D       PPPP+G+L  +        D  +M DWR+F++ GLL+ A+ME+KD+
Sbjct: 24   KAVAFSDDLVIPTLPPPPIGTLTGQ-GVSRGHTDDMDMGDWRRFREVGLLNEASMEKKDQ 83

Query: 67   EALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQDLAETEEIFKREQSAHLIAL 126
            EALLEK S L+ EL+ YQHN+GL+L+E K   SK++QL Q   E +EI KREQS+HL AL
Sbjct: 84   EALLEKISTLEKELYGYQHNMGLLLMENKELVSKHEQLNQAFQEAQEILKREQSSHLYAL 143

Query: 127  SEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKLTSQKKLADANALMHGIEEKS 186
            + VE R +NL+KAL  EKQ V  L+KAL E++EE ++I+L+S+ KL +ANAL+  +  +S
Sbjct: 144  TTVEQREENLRKALGLEKQCVQELEKALREIQEENSKIRLSSEAKLVEANALVASVNGRS 203

Query: 187  LELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQTEQISLVTRKEAHQVTSHKEK 246
             +++ K+ +AE KLAE  RKSSEL++R+ EVE RESVLQ E++S    +E+++ T  K++
Sbjct: 204  SDVENKIYSAESKLAEATRKSSELKLRLKEVETRESVLQQERLSFTKERESYEGTFQKQR 263

Query: 247  DGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQKEKDLEEIKKKIDQSSSILK 306
            + L +W++KLQ +EES++  +  LN +E+KVNE    +K KEK+LEE  +K+D S S  K
Sbjct: 264  EYLNEWEKKLQGKEESITEQKRNLNQREEKVNEIEKKLKLKEKELEEWNRKVDLSMSKSK 323

Query: 307  EKEDDVNRQLADVEAKEK----------------------------METQQLLHEQSALL 366
            E E+D+ ++L ++  KEK                             E Q+L+ +Q  +L
Sbjct: 324  ETEEDITKRLEELTTKEKEAHTLQITLLAKENELRAFEEKLIAREGTEIQKLIDDQKEVL 383

Query: 367  QKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKKEKLVKQEQALDKKLHRTKEK 426
              K  EFEL+ EE R+S++ E    +  ++R+ +EI+H +EKL K+ QA++KK  R  EK
Sbjct: 384  GSKMLEFELECEEIRKSLDKELQRKIEELERQKVEIDHSEEKLEKRNQAMNKKFDRVNEK 443

Query: 427  EGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQSLIDEIEKIRTASTQKEWQF 486
            E DLE KLK +K ++KI++A+E++  +E+ Q+L+D++SL+ L  EIEKIR   T+KE   
Sbjct: 444  EMDLEAKLKTIKEREKIIQAEEKRLSLEKQQLLSDKESLEDLQQEIEKIRAEMTKKEEMI 503

Query: 487  HEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTKEHDDLKQERVKFERDWEALD 546
             EE + L++ KEER E++RLQ +L  +IE  R+  + ++KE ++LKQE+ +FE++WE LD
Sbjct: 504  EEECKSLEIKKEEREEYLRLQSELKSQIEKSRVHEEFLSKEVENLKQEKERFEKEWEILD 563

Query: 547  EKRTEIHNGLGDLEELRKKLEILR-TEEERLRNEKTKMLIYMQRELDNVKQEKELFASTT 606
            EK+   +     + E ++K E  +  E ERL+ E++ + + + +ELD+++ ++E F +  
Sbjct: 564  EKQAVYNKERIRISEEKEKFERFQLLEGERLKKEESALRVQIMQELDDIRLQRESFEANM 623

Query: 607  RHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEELEKRRQERELAFEEERKRERN 666
             HE+ AL ++ + + ++++ D+E+ R++LE  LQ+ +E+ EK   +R   FE++R  E +
Sbjct: 624  EHERSALQEKVKLEQSKVIDDLEMMRRNLEIELQERKEQDEKDLLDRMAQFEDKRMAELS 683

Query: 667  EIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIADHLEIHQDIDKLNVLSKELKC 726
            +I + K    +E EE+ S+R  L+KE E +  ++ +L    +E+H DI +L+ LS  LK 
Sbjct: 684  DINHQKQALNREMEEMMSKRSALQKESEEIAKHKDKLKEQQVEMHNDISELSTLSINLKK 743

Query: 727  QREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMPAD----IREPDPLANLDVES 786
            +RE   ++R  FLAFV KLK C +CG  + +F++ DLQ+P++    I  P  + N    S
Sbjct: 744  RREVFGRERSRFLAFVQKLKDCGSCGQLVNDFVLSDLQLPSNDEVAILPPIGVLNDLPGS 803

Query: 787  LKLFQKELAASEFDSDSGG-------RMSWLRRCSRKILNLSPIKRIGQVV---PPVSMK 846
                         D D+ G        MS L++C+  I   SP KR+   +    P    
Sbjct: 804  SNASDSCNIKKSLDGDASGSGGSRRPSMSILQKCTSII--FSPSKRVEHGIDTGKPEQRL 863

Query: 847  LAADCTDLEAKEPSVSAGDVKRFSFSDNIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQP 906
             ++    +E K       D++    S +I   ++ +          DS+ +E SEGS+  
Sbjct: 864  SSSVAVGMETKGEKPLPVDLRLRPSSSSIPEEDEEY---------TDSRVQETSEGSQLS 923

Query: 907  DLKREKQKRGKGLNSRHRTHSMKVTVQDAKLFLGETVGQSDLNVLVQKSDSKFSNKETTN 966
            + +  ++ RG+               + AK  L  T            S  K ++ E ++
Sbjct: 924  EFQSSRRGRGR--------------PRKAKPALNPT------------SSVKHASLEESS 983

Query: 967  VRKRPRAESSTISVSEQDGDDSERCSDSITTGRQRKRQQKIASV-QAQGESRYNLRRPKI 1026
              +     S T   +   G    +  D   TG +R+RQQ +A + Q  G+  YNLRR K 
Sbjct: 984  KDELSGHVSVTSKKTTGGGGRKRQHIDDTATGGKRRRQQTVAVLPQTPGQRHYNLRRKKT 1043

Query: 1027 GGTASATEVSGNLTTGMEKEKDATMTAKVEPSGEAFVTSSLRSVDGENIKKADYVQLTTV 1086
                 A +V  N   G E + D   +A  + + E  V  +LR+   E    AD V     
Sbjct: 1044 VDQVPA-DVEDNAAAG-EDDADIAASAPSKDTVEETVVETLRARRIET--NADVVSAEN- 1103

Query: 1087 RTIYRSEDRVVEFESLRNPEDNASMEKLVAMDDLCDEVNNTSEYEDEDGNMIDDAEDEYD 1116
                  +  V   E   N + N             DE  + ++ +D + N  DD +D+ D
Sbjct: 1104 ----NGDVPVANVEPTVNEDTNED----------GDEEEDEAQDDDNEENQDDDDDDDGD 1128

BLAST of Cp4.1LG01g16820 vs. TAIR 10
Match: AT5G65770.1 (little nuclei4 )

HSP 1 Score: 206.8 bits (525), Expect = 8.8e-53
Identity = 237/954 (24.84%), Postives = 451/954 (47.27%), Query Frame = 0

Query: 41  WRKFKKAGLLDAAAMERKDREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQ 100
           W++ K AG  D  +++ +D+ AL+   ++L+SE++DYQHN+GL+LLEK   +S+Y+++  
Sbjct: 41  WKRLKDAG-FDEQSIKNRDKAALIAYIAKLESEVYDYQHNMGLLLLEKNELSSQYEEIKA 100

Query: 101 DLAETEEIFKREQSAHLIALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKL 160
            + E++    RE+SA++ AL+E + R ++LKK +   K+ +SSL+K L E++ E AE K+
Sbjct: 101 SVDESDLTHMREKSAYVSALAEAKKREESLKKDVGIAKECISSLEKTLHEMRAECAETKV 160

Query: 161 TSQKKLADANALMHGIEEKSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQT 220
           ++   +++A+ ++    +K  + + K+ AAE   AE NR     E ++ EVE+RE  L  
Sbjct: 161 SAGSTMSEAHVMIEDALKKLADAEAKMRAAEALQAEANRYHRIAERKLKEVESREDDLTR 220

Query: 221 EQISLVTRKEAHQVTSHKEKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQ 280
              S  +  E  +     E+  L + ++ LQ+  E L  ++  LN +E  +      + +
Sbjct: 221 RLASFKSECETKENEMVIERQTLNERRKSLQQEHERLLDAQVSLNQREDHIFARSQELAE 280

Query: 281 KEKDLEEIKKKIDQSSSILKEKEDDVNRQLA----------------------------D 340
            EK L+  K   ++     ++K+ ++   LA                             
Sbjct: 281 LEKGLDTAKTTFEEERKAFEDKKSNLEIALALCAKREEAVSERESSLLKKEQELLVAEEK 340

Query: 341 VEAKEKMETQQLLHEQSALLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKK 400
           + +KE    Q +L  Q  +L+K++ + E +LE K +SVE E      A + ++++I  ++
Sbjct: 341 IASKESELIQNVLANQEVILRKRKSDVEAELECKSKSVEVEIESKRRAWELREVDIKQRE 400

Query: 401 EKLVKQEQALDKKLHRTKEKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQ 460
           + + ++E  L+ +     EKE D+  K   L  K+K L A E     +   +  +++ L+
Sbjct: 401 DLVGEKEHDLEVQSRALAEKEKDITEKSFNLDEKEKNLVATEEDINRKTTMLEDEKERLR 460

Query: 461 SLIDEIEKIRTASTQKEWQFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTK 520
            L  E+++  T+   K  +     +KL+ +K E SE   L+ +L +E++  R Q   +  
Sbjct: 461 KLDLELQQSLTSLEDKRKRVDSATQKLEALKSETSELSTLEMKLKEELDDLRAQKLEMLA 520

Query: 521 EHDDLKQERVKFERDWEALDEKRTEIHNGLGDLEELRKKLEI-LRTEEERLRNEKTKMLI 580
           E D LK E+ KFE +WE +D KR E+      +   R+   + L+ E + ++ E+  +  
Sbjct: 521 EADRLKVEKAKFEAEWEHIDVKREELRKEAEYITRQREAFSMYLKDERDNIKEERDALRN 580

Query: 581 YMQRELDNVKQEKELFASTTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEEL 640
             + +++++ +E+E F +    E      + Q +    L  IE+Q+++LE  ++  +EEL
Sbjct: 581 QHKNDVESLNREREEFMNKMVEEHSEWLSKIQRERADFLLGIEMQKRELEYCIENKREEL 640

Query: 641 EKRRQERELAFEEERKRERNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIAD 700
           E   ++RE AFE+E+K E   I  LK++A KE E V  E  +L+ E+  + ++R+    +
Sbjct: 641 ENSSRDREKAFEQEKKLEEERIQSLKEMAEKELEHVQVELKRLDAERLEIKLDRERRERE 700

Query: 701 HLEIHQDIDKLNVLSKELKCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMP 760
             E+   +++L V  ++L+ QR  L  +R      +++LK  EN  V++ +  +  +Q+ 
Sbjct: 701 WAELKDSVEELKVQREKLETQRHMLRAERDEIRHEIEELKKLENLKVALDDMSMAKMQLS 760

Query: 761 ADIREPDPLANL--------DVESLKLFQKELAASE------------FDSDSGGRMSWL 820
              R  + ++ L        D   L+     ++ SE                S    SW+
Sbjct: 761 NLERSWEKVSALKQKVVSRDDELDLQNGVSTVSNSEDGYNSSMERQNGLTPSSATPFSWI 820

Query: 821 RRCSRKILNLSPIKRI-------GQVVPPVSMKLAADCTDLEAKEPSVSAGDVKRFSFSD 880
           +RC+  I   SP K            VP   +KL +   + +A    +S   V+R   + 
Sbjct: 821 KRCTNLIFKTSPEKSTLMHHYEEEGGVPSEKLKLESSRREEKAYTEGLSIA-VERLE-AG 880

Query: 881 NIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNSRHRTHSMKVTVQ 938
             R        T +   N   K +   + S + D +            +H   S +    
Sbjct: 881 RKRRGNTSGDETSEPSNNKKRKHDVTQKYSDEADTQSVISSPQNVPEDKHELPSSQTQTP 940

BLAST of Cp4.1LG01g16820 vs. TAIR 10
Match: AT5G65770.3 (little nuclei4 )

HSP 1 Score: 206.8 bits (525), Expect = 8.8e-53
Identity = 237/954 (24.84%), Postives = 451/954 (47.27%), Query Frame = 0

Query: 41  WRKFKKAGLLDAAAMERKDREALLEKASRLQSELFDYQHNLGLILLEKKMWASKYDQLGQ 100
           W++ K AG  D  +++ +D+ AL+   ++L+SE++DYQHN+GL+LLEK   +S+Y+++  
Sbjct: 41  WKRLKDAG-FDEQSIKNRDKAALIAYIAKLESEVYDYQHNMGLLLLEKNELSSQYEEIKA 100

Query: 101 DLAETEEIFKREQSAHLIALSEVETRRDNLKKALAAEKQHVSSLKKALCEVKEERAEIKL 160
            + E++    RE+SA++ AL+E + R ++LKK +   K+ +SSL+K L E++ E AE K+
Sbjct: 101 SVDESDLTHMREKSAYVSALAEAKKREESLKKDVGIAKECISSLEKTLHEMRAECAETKV 160

Query: 161 TSQKKLADANALMHGIEEKSLELQKKLNAAEVKLAEVNRKSSELEMRMHEVEARESVLQT 220
           ++   +++A+ ++    +K  + + K+ AAE   AE NR     E ++ EVE+RE  L  
Sbjct: 161 SAGSTMSEAHVMIEDALKKLADAEAKMRAAEALQAEANRYHRIAERKLKEVESREDDLTR 220

Query: 221 EQISLVTRKEAHQVTSHKEKDGLRKWQQKLQEREESLSRSRELLNDKEQKVNENGTIMKQ 280
              S  +  E  +     E+  L + ++ LQ+  E L  ++  LN +E  +      + +
Sbjct: 221 RLASFKSECETKENEMVIERQTLNERRKSLQQEHERLLDAQVSLNQREDHIFARSQELAE 280

Query: 281 KEKDLEEIKKKIDQSSSILKEKEDDVNRQLA----------------------------D 340
            EK L+  K   ++     ++K+ ++   LA                             
Sbjct: 281 LEKGLDTAKTTFEEERKAFEDKKSNLEIALALCAKREEAVSERESSLLKKEQELLVAEEK 340

Query: 341 VEAKEKMETQQLLHEQSALLQKKREEFELQLEEKRQSVENEGSITLGAIKRKDIEINHKK 400
           + +KE    Q +L  Q  +L+K++ + E +LE K +SVE E      A + ++++I  ++
Sbjct: 341 IASKESELIQNVLANQEVILRKRKSDVEAELECKSKSVEVEIESKRRAWELREVDIKQRE 400

Query: 401 EKLVKQEQALDKKLHRTKEKEGDLERKLKALKAKDKILKADERKHEVERLQMLADRDSLQ 460
           + + ++E  L+ +     EKE D+  K   L  K+K L A E     +   +  +++ L+
Sbjct: 401 DLVGEKEHDLEVQSRALAEKEKDITEKSFNLDEKEKNLVATEEDINRKTTMLEDEKERLR 460

Query: 461 SLIDEIEKIRTASTQKEWQFHEEREKLQVIKEERSEHVRLQCQLMQEIESYRLQNKIVTK 520
            L  E+++  T+   K  +     +KL+ +K E SE   L+ +L +E++  R Q   +  
Sbjct: 461 KLDLELQQSLTSLEDKRKRVDSATQKLEALKSETSELSTLEMKLKEELDDLRAQKLEMLA 520

Query: 521 EHDDLKQERVKFERDWEALDEKRTEIHNGLGDLEELRKKLEI-LRTEEERLRNEKTKMLI 580
           E D LK E+ KFE +WE +D KR E+      +   R+   + L+ E + ++ E+  +  
Sbjct: 521 EADRLKVEKAKFEAEWEHIDVKREELRKEAEYITRQREAFSMYLKDERDNIKEERDALRN 580

Query: 581 YMQRELDNVKQEKELFASTTRHEQQALSQQAQNKHNQLLQDIELQRKDLESHLQKSQEEL 640
             + +++++ +E+E F +    E      + Q +    L  IE+Q+++LE  ++  +EEL
Sbjct: 581 QHKNDVESLNREREEFMNKMVEEHSEWLSKIQRERADFLLGIEMQKRELEYCIENKREEL 640

Query: 641 EKRRQERELAFEEERKRERNEIFYLKDIALKEKEEVSSERHQLEKEKEVVTMNRKELIAD 700
           E   ++RE AFE+E+K E   I  LK++A KE E V  E  +L+ E+  + ++R+    +
Sbjct: 641 ENSSRDREKAFEQEKKLEEERIQSLKEMAEKELEHVQVELKRLDAERLEIKLDRERRERE 700

Query: 701 HLEIHQDIDKLNVLSKELKCQREQLIQDRICFLAFVDKLKSCENCGVSIKEFMVPDLQMP 760
             E+   +++L V  ++L+ QR  L  +R      +++LK  EN  V++ +  +  +Q+ 
Sbjct: 701 WAELKDSVEELKVQREKLETQRHMLRAERDEIRHEIEELKKLENLKVALDDMSMAKMQLS 760

Query: 761 ADIREPDPLANL--------DVESLKLFQKELAASE------------FDSDSGGRMSWL 820
              R  + ++ L        D   L+     ++ SE                S    SW+
Sbjct: 761 NLERSWEKVSALKQKVVSRDDELDLQNGVSTVSNSEDGYNSSMERQNGLTPSSATPFSWI 820

Query: 821 RRCSRKILNLSPIKRI-------GQVVPPVSMKLAADCTDLEAKEPSVSAGDVKRFSFSD 880
           +RC+  I   SP K            VP   +KL +   + +A    +S   V+R   + 
Sbjct: 821 KRCTNLIFKTSPEKSTLMHHYEEEGGVPSEKLKLESSRREEKAYTEGLSIA-VERLE-AG 880

Query: 881 NIRVAEDRHAHTFDDFGNVDSKFEEASEGSKQPDLKREKQKRGKGLNSRHRTHSMKVTVQ 938
             R        T +   N   K +   + S + D +            +H   S +    
Sbjct: 881 RKRRGNTSGDETSEPSNNKKRKHDVTQKYSDEADTQSVISSPQNVPEDKHELPSSQTQTP 940