Cp4.1LG01g16740 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g16740
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionelongation factor 1-gamma-like
LocationCp4.1LG01: 10548828 .. 10551832 (+)
RNA-Seq ExpressionCp4.1LG01g16740
SyntenyCp4.1LG01g16740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGGGAAAAAAAGAATAAAAAGAACGAACGAGGAGTCCAATATTCCATATATGTAAATTTTATATTGCCTTCGGAAATTCTGCATCGAACTGCAAAACCCTACTCCACTTCTCGACTTTTTCGCCTCCAACTGCTTCTCGTGATTCTGCAGTTAAGCTCCCTCTCTCAGGTCTCTAATTCTCCATTTCAATCTTCAACGTAATCATTATCGATTTCTATGTCTTCTATTTGCTAATCAAAAGTGTTTTTCATTGTTCTTTGCCATGGCGGAAACTACTTCCTTGTAACTTCCATTTACTGTAGATTACTCACTTGATTTTGTTCATTTTGTTATGTCGCCAGATCTGAAGCCAGAGCGTGGAGCATTTCTCCGTTCAGCCATGGCACTGGTGTGTGATCGATACTTGTATTGAACTAATTTTCAATGAATTGAATTCAAAAGAAATTTATCTCGAATGTTGTCTGATGTATATGACTTGGGTTTGATTGCAGGTGATACACGCAGGAAAGACGAATAAAAATTCTTTCAAGGCTCTCATTGCTGCAGAGTACAATGGAGTCGAAGTGAAATTGATCCCAGACTTTGAGATGGGAGTCTCAAACAAGACTCCGGAGTTTATCAAGATGAACCCTATTGGGAAGGTTGGTTTCTGATTTGATAAGCGGAAATTCGTCTTGTCAAAATGATCTTGCTTACTTGTTATAGATGAATTAACATTCAATGAGAATTTCTCGCATTTTTAGGTTCCTGTTTTAGAAACGCCCGATGGCCCCATATTTGAGAGCAATGCGATAGCCCGTTATGGTAAATGATGCGAACCATTTTCTAGACCTCGGGTTGGTTAACTGATTGTGTTAAAACTGACTTGCTTGTGTATTTGATGATGCAGTTGCACGCTTGAAGGCTGATAGTCCTCTGTTTGGTTCATCTTCGATAGATTATGTGAGATGAGTTGCATTAACATTTTTATTCTTTTTGTATTAAACCTACATTTTACGACCGTGGGAAATTATGCTTATTAATTATCATGTTTGTTGCTTCTGATTGTGTTTCAGGGGCATATTGAACAATGGATTGATTTTGCATCATTAGAGATAGATCCTAATATTTTGACTTGGTATAGACCTAGAATTGGGAGAGCTGCTTATCTTCCACCTGTAAGTAACTCTTGTGGCCATTTGATTAATTTCTCTGGTTTATTTTGCGCTTGTGTTCATACTTAAAATGCTTCCTTTCGGATTATTTGAATTCTTGTGGCCAATTGAGCTGATTATCTTTCTCCTTTCTTGGTCAAGTCCTAGCTTCTATGAGTGCAAACACCTGTATATTCATCATTTTTTTTTCTGATAATGCGGTTATATCTGGATTGAATGCCATCTCATATGATTTGATAACATTTCTTCCCGTATTAAGGTTGAGGAAGCTGCAATTGCTGCATTGAAGAGAGGTTTGGGAGCTTTGAATACGCACCTTGCTTCAAACACATACCTAGTTGGTCATTTTGTAACATTAGCTGACATCATCATGACTTGCAACCTCTTATTGGGTTTTACTAAGTTAATGACTAAGAGCTTTACCTCAGAGTTCCCACATGTTGAGAGGTACTTCTGGACCTTAGTCAATCAACCAAACTTCAAAAAAATTCTGGGTGAGGTGAGGCAGGCAGAGTCAGTTCCACCTGTTCAATCTGCTAAGAAACCTGATGAATCTGCTAAGCAAAAGCACAAAGATGAGGTAAAGAAAGAACCTAAAAAGGAAGCAGAAAAAGAGAAGCCCAAGGCGGTAGTTGGCGAGGGAGAGGATGAGGCACCAAAGCCTAAACCCAAAAATCCTCTCGATTTGCTTCCTCCCAGTAAGATGATTCTGGACGAGTGGAAGCGGCTGTACTCGAATACCAAAACCAACTTCCGGGAGGTTGCAATCAAAGGTATGGAACCCTTTCAGGTGAATTGTTTTGGAGAATGATCGCATCAATGATTGATACCCGCCATATAAGTGGGAACCTTTATTGTCATTTGTTGAGTAATGGTTCGATGACATGAAAGCATGCAAAACTTTTACGGAAAATGAACCTTGTCTTCTCTTTGTGTGAATAACAACCATTTATTTACTATCTTTAGAGCATTCGTATCTACAGTCACTAGCACTTAATTTTTATTTCTTCAAACTTGTTTATTTCTTCAAGTTTTCTGACTACTCCACTGATGATCTGGATAATGTAATTGTTAGCTTTCTCTTGCTTGCATTTAGTTTCACTACATATAATAAACAAGCATTCTCCTAAATTTGTTGCAGGATTTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACGACGATGAGAATACTGTTTCGTTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAGCGGATGGATCTCGCACGTAAGTATGCTTTCGGGAAGATGTTAGTGATTGGATCCGAACCTCCATTTAAGGTGAAGGGATTGTGGCTGTTCCGTGGGCAAGAGATCCCAAAGTTCGTCCTGGACGAGTGCTATGACATGGAACTGTATGAATGGAGGAAGGTGGATATATCAGACGAGGCACAAAAGGAACGCGTGAACCAGATGATTGAAGATCATGAGCCTTTTGAAGGAGAGGCTTTGTTGGACGCCAAATGCTTCAAGTGATGAAGCAATTATCTTAAGGGGTGTTGTTTTAGTTTCTTTTCGAGTCGAGAGTTCCAAGTATCATCATCATCATCATATATCATCGTTTACTAGTCCTTATTTCTTATTTCGGTTCGAGTGTGATTAGACAGATATACCCCTCCCAAAGGGGGGAAAAACAGCAAGTAAAAATCTCCCCGTGTTAATTCTTCTCCTTTTTTGTTCTTCATGAAACTAAAAGTCTATGTTTCTACAATACACTATTCAAGGCTTTGTTCATTTAAAATGTATGAAGAGGATGTGTGGAAATAAAATGTTTAAAAATTATATAAATGTGTTTGATATTGAT

mRNA sequence

GAGGGGAAAAAAAGAATAAAAAGAACGAACGAGGAGTCCAATATTCCATATATGTAAATTTTATATTGCCTTCGGAAATTCTGCATCGAACTGCAAAACCCTACTCCACTTCTCGACTTTTTCGCCTCCAACTGCTTCTCGTGATTCTGCAGTTAAGCTCCCTCTCTCAGATCTGAAGCCAGAGCGTGGAGCATTTCTCCGTTCAGCCATGGCACTGGTGATACACGCAGGAAAGACGAATAAAAATTCTTTCAAGGCTCTCATTGCTGCAGAGTACAATGGAGTCGAAGTGAAATTGATCCCAGACTTTGAGATGGGAGTCTCAAACAAGACTCCGGAGTTTATCAAGATGAACCCTATTGGGAAGGTTCCTGTTTTAGAAACGCCCGATGGCCCCATATTTGAGAGCAATGCGATAGCCCGTTATGTTGCACGCTTGAAGGCTGATAGTCCTCTGTTTGGTTCATCTTCGATAGATTATGGGCATATTGAACAATGGATTGATTTTGCATCATTAGAGATAGATCCTAATATTTTGACTTGGTATAGACCTAGAATTGGGAGAGCTGCTTATCTTCCACCTGTTGAGGAAGCTGCAATTGCTGCATTGAAGAGAGGTTTGGGAGCTTTGAATACGCACCTTGCTTCAAACACATACCTAGTTGGTCATTTTGTAACATTAGCTGACATCATCATGACTTGCAACCTCTTATTGGGTTTTACTAAGTTAATGACTAAGAGCTTTACCTCAGAGTTCCCACATGTTGAGAGGTACTTCTGGACCTTAGTCAATCAACCAAACTTCAAAAAAATTCTGGGTGAGGTGAGGCAGGCAGAGTCAGTTCCACCTGTTCAATCTGCTAAGAAACCTGATGAATCTGCTAAGCAAAAGCACAAAGATGAGGTAAAGAAAGAACCTAAAAAGGAAGCAGAAAAAGAGAAGCCCAAGGCGGTAGTTGGCGAGGGAGAGGATGAGGCACCAAAGCCTAAACCCAAAAATCCTCTCGATTTGCTTCCTCCCAGTAAGATGATTCTGGACGAGTGGAAGCGGCTGTACTCGAATACCAAAACCAACTTCCGGGAGGTTGCAATCAAAGGATTTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACGACGATGAGAATACTGTTTCGTTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAGCGGATGGATCTCGCACGTAAGTATGCTTTCGGGAAGATGTTAGTGATTGGATCCGAACCTCCATTTAAGGTGAAGGGATTGTGGCTGTTCCGTGGGCAAGAGATCCCAAAGTTCGTCCTGGACGAGTGCTATGACATGGAACTGTATGAATGGAGGAAGGTGGATATATCAGACGAGGCACAAAAGGAACGCGTGAACCAGATGATTGAAGATCATGAGCCTTTTGAAGGAGAGGCTTTGTTGGACGCCAAATGCTTCAAGTGATGAAGCAATTATCTTAAGGGGTGTTGTTTTAGTTTCTTTTCGAGTCGAGAGTTCCAAGTATCATCATCATCATCATATATCATCGTTTACTAGTCCTTATTTCTTATTTCGGTTCGAGTGTGATTAGACAGATATACCCCTCCCAAAGGGGGGAAAAACAGCAAGTAAAAATCTCCCCGTGTTAATTCTTCTCCTTTTTTGTTCTTCATGAAACTAAAAGTCTATGTTTCTACAATACACTATTCAAGGCTTTGTTCATTTAAAATGTATGAAGAGGATGTGTGGAAATAAAATGTTTAAAAATTATATAAATGTGTTTGATATTGAT

Coding sequence (CDS)

ATGGCACTGGTGATACACGCAGGAAAGACGAATAAAAATTCTTTCAAGGCTCTCATTGCTGCAGAGTACAATGGAGTCGAAGTGAAATTGATCCCAGACTTTGAGATGGGAGTCTCAAACAAGACTCCGGAGTTTATCAAGATGAACCCTATTGGGAAGGTTCCTGTTTTAGAAACGCCCGATGGCCCCATATTTGAGAGCAATGCGATAGCCCGTTATGTTGCACGCTTGAAGGCTGATAGTCCTCTGTTTGGTTCATCTTCGATAGATTATGGGCATATTGAACAATGGATTGATTTTGCATCATTAGAGATAGATCCTAATATTTTGACTTGGTATAGACCTAGAATTGGGAGAGCTGCTTATCTTCCACCTGTTGAGGAAGCTGCAATTGCTGCATTGAAGAGAGGTTTGGGAGCTTTGAATACGCACCTTGCTTCAAACACATACCTAGTTGGTCATTTTGTAACATTAGCTGACATCATCATGACTTGCAACCTCTTATTGGGTTTTACTAAGTTAATGACTAAGAGCTTTACCTCAGAGTTCCCACATGTTGAGAGGTACTTCTGGACCTTAGTCAATCAACCAAACTTCAAAAAAATTCTGGGTGAGGTGAGGCAGGCAGAGTCAGTTCCACCTGTTCAATCTGCTAAGAAACCTGATGAATCTGCTAAGCAAAAGCACAAAGATGAGGTAAAGAAAGAACCTAAAAAGGAAGCAGAAAAAGAGAAGCCCAAGGCGGTAGTTGGCGAGGGAGAGGATGAGGCACCAAAGCCTAAACCCAAAAATCCTCTCGATTTGCTTCCTCCCAGTAAGATGATTCTGGACGAGTGGAAGCGGCTGTACTCGAATACCAAAACCAACTTCCGGGAGGTTGCAATCAAAGGATTTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACGACGATGAGAATACTGTTTCGTTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAGCGGATGGATCTCGCACGTAAGTATGCTTTCGGGAAGATGTTAGTGATTGGATCCGAACCTCCATTTAAGGTGAAGGGATTGTGGCTGTTCCGTGGGCAAGAGATCCCAAAGTTCGTCCTGGACGAGTGCTATGACATGGAACTGTATGAATGGAGGAAGGTGGATATATCAGACGAGGCACAAAAGGAACGCGTGAACCAGATGATTGAAGATCATGAGCCTTTTGAAGGAGAGGCTTTGTTGGACGCCAAATGCTTCAAGTGA

Protein sequence

MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETPDGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRAAYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFTSEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKEAEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWDMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVKGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKCFK
Homology
BLAST of Cp4.1LG01g16740 vs. ExPASy Swiss-Prot
Match: Q9FUM1 (Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 7.4e-207
Identity = 352/424 (83.02%), Postives = 383/424 (90.33%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+HAGKTNKN+FK LI AEY GV+V+L PDFEMGV+NKTPE++K+NPIGKVP+LETP
Sbjct: 1   MALVLHAGKTNKNAFKTLIVAEYTGVKVELAPDFEMGVTNKTPEYLKLNPIGKVPLLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKAD+PL GSS IDY HIEQWIDF SLEID NI++W+RPR G A
Sbjct: 61  DGPIFESNAIARYVARLKADNPLIGSSLIDYAHIEQWIDFGSLEIDANIISWFRPRFGYA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            YLPP EEAAI+ALKR LGALNTHLASNTYLVGHFVTLADII+TCNL  GFTKLM KSFT
Sbjct: 121 VYLPPAEEAAISALKRALGALNTHLASNTYLVGHFVTLADIIVTCNLFFGFTKLMIKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQP FKK+LG+V+Q ESVPPV SAKKP +  + K K   K+EPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPKFKKVLGDVKQTESVPPVPSAKKPSQPKETKSK--AKEEPKKE 240

Query: 241 AEKE--KPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGF 300
           A+KE  KPKA   E  +EAPKPKPKNPLDLLPPS M+LD+WKRLYSNTKTNFREVAIKGF
Sbjct: 241 AKKEPAKPKAEAAEEVEEAPKPKPKNPLDLLPPSNMVLDDWKRLYSNTKTNFREVAIKGF 300

Query: 301 WDMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 360
           WDMYDPEGYSLWFC+YKY+DENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK
Sbjct: 301 WDMYDPEGYSLWFCEYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 360

Query: 361 VKGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDA 420
           VKGLWLFRGQEIP FV++ECYDMELY W KVD+SDE QKERVNQ+IED EPFEGEALLDA
Sbjct: 361 VKGLWLFRGQEIPPFVMEECYDMELYNWTKVDLSDENQKERVNQVIEDQEPFEGEALLDA 420

Query: 421 KCFK 423
           KCFK
Sbjct: 421 KCFK 422

BLAST of Cp4.1LG01g16740 vs. ExPASy Swiss-Prot
Match: Q6YW46 (Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220500 PE=2 SV=2)

HSP 1 Score: 682.2 bits (1759), Expect = 3.8e-195
Identity = 339/423 (80.14%), Postives = 373/423 (88.18%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+HAG  NKN+FKALIAAEY+GV+V+L+ +F+MGVSNKTPEF+KMNPIGK+PVLETP
Sbjct: 1   MALVLHAGSGNKNAFKALIAAEYSGVKVELVKNFQMGVSNKTPEFLKMNPIGKIPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGP+FESNAIARYV R KAD+PL+GSS I+Y HIEQW DF++ E+D NI  W  PR+G A
Sbjct: 61  DGPVFESNAIARYVTRSKADNPLYGSSLIEYAHIEQWNDFSATEVDANIGKWLYPRLGIA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            Y+   EEAAIAALKR LGALNTHLASNTYLVGH VTLADI+MTCNL +GF ++MTKSFT
Sbjct: 121 PYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWT+VNQPNFKK+LG+V+QAESVPPVQ    P +  K K   E KKE  KE
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVLGDVKQAESVPPVQKKAPPPKEQKPK---EAKKEAPKE 240

Query: 241 AEKEKPKAV-VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW 300
           A   KPKAV   E E+EAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW
Sbjct: 241 A--PKPKAVEKPEEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW 300

Query: 301 DMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKV 360
           DMYDPEGYSLWFCDYKY+DENTVSFVT+NKVGGFLQRMDL RKYAFGKMLVIGSEPPFKV
Sbjct: 301 DMYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFKV 360

Query: 361 KGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAK 420
           KGLWLFRG EIPKFV+DE YDMELYEW KVDISDEAQKERV+ MIED EPFEGE+LLDAK
Sbjct: 361 KGLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGESLLDAK 418

Query: 421 CFK 423
           CFK
Sbjct: 421 CFK 418

BLAST of Cp4.1LG01g16740 vs. ExPASy Swiss-Prot
Match: Q9ZRI7 (Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220600 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.1e-189
Identity = 332/422 (78.67%), Postives = 369/422 (87.44%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H    NKN+FKALIAAEY+GV+V+L  +F+MGVSNKTPE++KMNPIGKVP+LETP
Sbjct: 1   MALVLHTFDGNKNAFKALIAAEYSGVKVELAKNFQMGVSNKTPEYLKMNPIGKVPILETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGP+FESNAIARYV R K+D+PL+GSS I+Y HIEQWIDF++ E+D N   W  PR+G A
Sbjct: 61  DGPVFESNAIARYVTRSKSDNPLYGSSLIEYAHIEQWIDFSATEVDANTGKWLFPRLGFA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            Y+   EEAAIAALKR LGALNTHLASNTYLVGH VTLADI+MTCNL +GF ++MTK+FT
Sbjct: 121 PYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKNFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWT+VNQPNFKK++G+V+QA+SVP VQ  KK     +QK K E KKE  KE
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVMGDVKQADSVPQVQ--KKAAAPKEQKPK-EAKKEAPKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           A K K  A   E E+EAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 APKPK-AAEKPEEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKY+DENTVSFVT+NKVGGFLQRMDL RKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRG EIPKFV+DE YDMELYEW KVDISDEAQKERV+ MIED EPFEGEALLDAKC
Sbjct: 361 GLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGEALLDAKC 418

Query: 421 FK 423
           FK
Sbjct: 421 FK 418

BLAST of Cp4.1LG01g16740 vs. ExPASy Swiss-Prot
Match: Q5Z627 (Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0571400 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 9.1e-189
Identity = 325/422 (77.01%), Postives = 363/422 (86.02%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H G  NKN+FKALIAAEY GV+V+L  +FEMGVSNKTPEF+KMNP+GK+PVLETP
Sbjct: 1   MALVLHCGSGNKNAFKALIAAEYTGVKVELTKNFEMGVSNKTPEFLKMNPLGKIPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +G +FESNAIARYVARLK +S L GSS IDY HIEQW+DF++ E+D NI  W  PR+G  
Sbjct: 61  EGAVFESNAIARYVARLKDNSSLCGSSLIDYSHIEQWMDFSATEVDANIGRWLYPRLGFG 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            Y+P +EE AI +LKR LGALNTHLASNTYLVGH VTLADI+MTCNL  GF +++ KSFT
Sbjct: 121 PYVPVLEEFAITSLKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYYGFVRILIKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWT+VNQPNFKK++G+ +QAESVPPVQ    P + +K K   E KKE  KE
Sbjct: 181 SEFPHVERYFWTMVNQPNFKKVIGDFKQAESVPPVQKKAAPPKESKAK---EAKKEAPKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           A K K +A     E+EAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFRE+AIKGFWD
Sbjct: 241 APKPKVEA---SEEEEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREIAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKY+DENTVSFVT+NKVGGFLQRMDL RKYAFGKMLVIGS PPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSTPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQ+IPKFV+DE YDMELYEW KVD+SDEAQKERVN MIED EPFEGE LLDAKC
Sbjct: 361 GLWLFRGQDIPKFVMDEVYDMELYEWTKVDLSDEAQKERVNAMIEDQEPFEGEDLLDAKC 416

Query: 421 FK 423
           FK
Sbjct: 421 FK 416

BLAST of Cp4.1LG01g16740 vs. ExPASy Swiss-Prot
Match: Q9FVT2 (Probable elongation factor 1-gamma 2 OS=Arabidopsis thaliana OX=3702 GN=At1g57720 PE=1 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.9e-178
Identity = 313/423 (74.00%), Postives = 354/423 (83.69%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H  K NK + KALIAAEY GV+++   DF+MGV+NK+PEF+KMNPIGKVPVLETP
Sbjct: 1   MALVMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +GPIFESNAIARYV+R   D+ L GSS I+Y HIEQWIDF+SLEID N+L W+ PR+G A
Sbjct: 61  EGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            +  P EEAAI+ALKRGL ALNTHLASNT+LVGH VTLADI+  CNL LGF  +MTK FT
Sbjct: 121 PFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           S FPHVERYFWT+VNQP FKK+LG+ +Q E+VPPV + K P  +       + K+EPKK 
Sbjct: 181 SAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKKAPQPA-------KPKEEPKKA 240

Query: 241 AE-KEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW 300
           A   E PK      E+EAPKPK KNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFW
Sbjct: 241 APVAEAPKPA---EEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFW 300

Query: 301 DMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKV 360
           DMYDPEGYSLWFCDYKY+DEN VSFVTLNKVGGFLQRMDLARKY+FGKML+ GSE PFKV
Sbjct: 301 DMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKV 360

Query: 361 KGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAK 420
           KGLWLFRG EIPKF++DE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAK
Sbjct: 361 KGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAK 413

Query: 421 CFK 423
           CFK
Sbjct: 421 CFK 413

BLAST of Cp4.1LG01g16740 vs. NCBI nr
Match: XP_023530487.1 (elongation factor 1-gamma-like [Cucurbita pepo subsp. pepo] >XP_023530496.1 elongation factor 1-gamma-like [Cucurbita pepo subsp. pepo] >XP_023530503.1 elongation factor 1-gamma-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 842 bits (2174), Expect = 7.85e-308
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. NCBI nr
Match: KAG7032457.1 (hypothetical protein SDJN02_06505 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 838 bits (2165), Expect = 1.85e-306
Identity = 420/422 (99.53%), Postives = 421/422 (99.76%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 121 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. NCBI nr
Match: XP_022929968.1 (elongation factor 1-gamma-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 832 bits (2149), Expect = 5.08e-304
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 121 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. NCBI nr
Match: XP_022929967.1 (elongation factor 1-gamma-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 832 bits (2149), Expect = 3.01e-303
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 48  MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 107

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 108 DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 167

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 168 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 227

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 228 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 287

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 288 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 347

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 348 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 407

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 408 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 467

Query: 421 FK 422
           FK
Sbjct: 468 FK 469

BLAST of Cp4.1LG01g16740 vs. NCBI nr
Match: XP_022929966.1 (elongation factor 1-gamma-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 832 bits (2149), Expect = 3.77e-303
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 54  MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 113

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 114 DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 173

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 174 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 233

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 234 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 293

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 294 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 353

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 354 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 413

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 414 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 473

Query: 421 FK 422
           FK
Sbjct: 474 FK 475

BLAST of Cp4.1LG01g16740 vs. ExPASy TrEMBL
Match: A0A6J1ETQ1 (elongation factor 1-gamma-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436431 PE=4 SV=1)

HSP 1 Score: 832 bits (2149), Expect = 2.46e-304
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 121 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. ExPASy TrEMBL
Match: A0A6J1EP33 (elongation factor 1-gamma-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436431 PE=4 SV=1)

HSP 1 Score: 832 bits (2149), Expect = 1.46e-303
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 48  MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 107

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 108 DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 167

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 168 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 227

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 228 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 287

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 288 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 347

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 348 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 407

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 408 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 467

Query: 421 FK 422
           FK
Sbjct: 468 FK 469

BLAST of Cp4.1LG01g16740 vs. ExPASy TrEMBL
Match: A0A6J1EPL4 (elongation factor 1-gamma-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436431 PE=4 SV=1)

HSP 1 Score: 832 bits (2149), Expect = 1.83e-303
Identity = 417/422 (98.82%), Postives = 418/422 (99.05%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 54  MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 113

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA
Sbjct: 114 DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 173

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT
Sbjct: 174 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 233

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 234 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 293

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
            EKEKPKA VGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 294 VEKEKPKAEVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 353

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 354 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 413

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIP FVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 414 GLWLFRGQEIPTFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 473

Query: 421 FK 422
           FK
Sbjct: 474 FK 475

BLAST of Cp4.1LG01g16740 vs. ExPASy TrEMBL
Match: A0A6J1JYI5 (elongation factor 1-gamma-like OS=Cucurbita maxima OX=3661 GN=LOC111490019 PE=4 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 2.02e-303
Identity = 416/422 (98.58%), Postives = 420/422 (99.53%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEID NILTWYRPRIGRA
Sbjct: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDSNILTWYRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAIAALKR LGALNTHLASNTYLVG+FVTLADIIMTCNLLLGFTKLMT+SFT
Sbjct: 121 AYLPPVEEAAIAALKRALGALNTHLASNTYLVGYFVTLADIIMTCNLLLGFTKLMTRSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVKQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFC+YKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCNYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. ExPASy TrEMBL
Match: A0A6J1DXR1 (elongation factor 1-gamma-like OS=Momordica charantia OX=3673 GN=LOC111025489 PE=4 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 1.37e-285
Identity = 391/422 (92.65%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+HAGKTNKN+FKALIAAEYNGV+V+L P+FEMGVSNKTPEFIKMNPIGKVPVLETP
Sbjct: 1   MALVLHAGKTNKNAFKALIAAEYNGVQVELTPNFEMGVSNKTPEFIKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           DGPIFESNAIARYV RLKADSPL+GSS IDYG IEQWIDFASLEID NIL W+RPRIGRA
Sbjct: 61  DGPIFESNAIARYVTRLKADSPLYGSSLIDYGRIEQWIDFASLEIDSNILAWFRPRIGRA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
           AYLPPVEEAAI+ALKR LGALNT+LASNTYLVGH VTLADIIMTCNL LGFTKL+TKSFT
Sbjct: 121 AYLPPVEEAAISALKRALGALNTNLASNTYLVGHSVTLADIIMTCNLYLGFTKLLTKSFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWTLVNQPNFKKILGEV+QAE  PPVQSAKKPD+SAKQKHKDEVKKEPK E
Sbjct: 181 SEFPHVERYFWTLVNQPNFKKILGEVKQAELAPPVQSAKKPDDSAKQKHKDEVKKEPKNE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           AEKEKPKA  GEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD
Sbjct: 241 AEKEKPKAAAGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKY+DENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRGQEIPKFV+DECYDMELYEWRKVDI DE QKERVNQMIED EPFEGEALLDAKC
Sbjct: 361 GLWLFRGQEIPKFVVDECYDMELYEWRKVDILDELQKERVNQMIEDQEPFEGEALLDAKC 420

Query: 421 FK 422
           FK
Sbjct: 421 FK 422

BLAST of Cp4.1LG01g16740 vs. TAIR 10
Match: AT1G57720.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 626.7 bits (1615), Expect = 1.3e-179
Identity = 313/423 (74.00%), Postives = 354/423 (83.69%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H  K NK + KALIAAEY GV+++   DF+MGV+NK+PEF+KMNPIGKVPVLETP
Sbjct: 1   MALVMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +GPIFESNAIARYV+R   D+ L GSS I+Y HIEQWIDF+SLEID N+L W+ PR+G A
Sbjct: 61  EGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            +  P EEAAI+ALKRGL ALNTHLASNT+LVGH VTLADI+  CNL LGF  +MTK FT
Sbjct: 121 PFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           S FPHVERYFWT+VNQP FKK+LG+ +Q E+VPPV + K P  +       + K+EPKK 
Sbjct: 181 SAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKKAPQPA-------KPKEEPKKA 240

Query: 241 AE-KEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW 300
           A   E PK      E+EAPKPK KNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFW
Sbjct: 241 APVAEAPKPA---EEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFW 300

Query: 301 DMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKV 360
           DMYDPEGYSLWFCDYKY+DEN VSFVTLNKVGGFLQRMDLARKY+FGKML+ GSE PFKV
Sbjct: 301 DMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKV 360

Query: 361 KGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAK 420
           KGLWLFRG EIPKF++DE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAK
Sbjct: 361 KGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAK 413

Query: 421 CFK 423
           CFK
Sbjct: 421 CFK 413

BLAST of Cp4.1LG01g16740 vs. TAIR 10
Match: AT1G57720.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 626.7 bits (1615), Expect = 1.3e-179
Identity = 313/423 (74.00%), Postives = 354/423 (83.69%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H  K NK + KALIAAEY GV+++   DF+MGV+NK+PEF+KMNPIGKVPVLETP
Sbjct: 1   MALVMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +GPIFESNAIARYV+R   D+ L GSS I+Y HIEQWIDF+SLEID N+L W+ PR+G A
Sbjct: 61  EGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYA 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            +  P EEAAI+ALKRGL ALNTHLASNT+LVGH VTLADI+  CNL LGF  +MTK FT
Sbjct: 121 PFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           S FPHVERYFWT+VNQP FKK+LG+ +Q E+VPPV + K P  +       + K+EPKK 
Sbjct: 181 SAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKKAPQPA-------KPKEEPKKA 240

Query: 241 AE-KEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFW 300
           A   E PK      E+EAPKPK KNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFW
Sbjct: 241 APVAEAPKPA---EEEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFW 300

Query: 301 DMYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKV 360
           DMYDPEGYSLWFCDYKY+DEN VSFVTLNKVGGFLQRMDLARKY+FGKML+ GSE PFKV
Sbjct: 301 DMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKV 360

Query: 361 KGLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAK 420
           KGLWLFRG EIPKF++DE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAK
Sbjct: 361 KGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAK 413

Query: 421 CFK 423
           CFK
Sbjct: 421 CFK 413

BLAST of Cp4.1LG01g16740 vs. TAIR 10
Match: AT1G09640.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 616.3 bits (1588), Expect = 1.8e-176
Identity = 309/422 (73.22%), Postives = 351/422 (83.18%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H  K NK++ KALIAAEY GV++ +  DF+MGV+NKTP F+KMNPIGKVPVLETP
Sbjct: 1   MALVLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +G +FESNAIARYV+RL  D+ L GSS I+Y  IEQWIDF+SLEI  +IL W+ PR+G  
Sbjct: 61  EGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFM 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            Y  P EE AI+ LKR L ALNTHL SNTYLVGH +TLADII  CNL LGF  +MTK FT
Sbjct: 121 PYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWT+VNQPNF K+LG+V+Q E+VPP+ S KK  + AK K + + K+ P  E
Sbjct: 181 SEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIAS-KKAAQPAKPKEEPKKKEAPVAE 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
           A K      + E E+EAPKPK KNPLDLLPPS M+LD+WKRLYSNTK+NFREVAIKGFWD
Sbjct: 241 APK------LAE-EEEAPKPKAKNPLDLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWD 300

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
           MYDPEGYSLWFCDYKY+DEN VSFVTLNKVGGFLQRMDLARKY+FGKML+ GSE PFKVK
Sbjct: 301 MYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVK 360

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRG EIPKF++DE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKC
Sbjct: 361 GLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKC 414

Query: 421 FK 423
           FK
Sbjct: 421 FK 414

BLAST of Cp4.1LG01g16740 vs. TAIR 10
Match: AT1G09640.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-92
Identity = 196/422 (46.45%), Postives = 221/422 (52.37%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           MALV+H  K NK++ KALIAAEY GV++ +  DF+MGV+NKTP F+KMNPIGKVPVLETP
Sbjct: 1   MALVLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETP 60

Query: 61  DGPIFESNAIARYVARLKADSPLFGSSSIDYGHIEQWIDFASLEIDPNILTWYRPRIGRA 120
           +G +FESNAIARYV+RL  D+ L GSS I+Y  IEQWIDF+SLEI  +IL W+ PR+G  
Sbjct: 61  EGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFM 120

Query: 121 AYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADIIMTCNLLLGFTKLMTKSFT 180
            Y  P EE AI+ LKR L ALNTHL SNTYLVGH +TLADII  CNL LGF  +MTK FT
Sbjct: 121 PYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFT 180

Query: 181 SEFPHVERYFWTLVNQPNFKKILGEVRQAESVPPVQSAKKPDESAKQKHKDEVKKEPKKE 240
           SEFPHVERYFWT+VNQPNF                                         
Sbjct: 181 SEFPHVERYFWTVVNQPNFT---------------------------------------- 240

Query: 241 AEKEKPKAVVGEGEDEAPKPKPKNPLDLLPPSKMILDEWKRLYSNTKTNFREVAIKGFWD 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 265

Query: 301 MYDPEGYSLWFCDYKYDDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFKVK 360
                                                                    KVK
Sbjct: 301 ---------------------------------------------------------KVK 265

Query: 361 GLWLFRGQEIPKFVLDECYDMELYEWRKVDISDEAQKERVNQMIEDHEPFEGEALLDAKC 420
           GLWLFRG EIPKF++DE YDMELYEW KVDISDEAQKERV+QMIED EPFEGEALLDAKC
Sbjct: 361 GLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEALLDAKC 265

Query: 421 FK 423
           FK
Sbjct: 421 FK 265

BLAST of Cp4.1LG01g16740 vs. TAIR 10
Match: AT2G30870.1 (glutathione S-transferase PHI 10 )

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-13
Identity = 53/168 (31.55%), Postives = 83/168 (49.40%), Query Frame = 0

Query: 1   MALVIHAGKTNKNSFKALIAAEYNGVEVKLIPDFEMGVSNKTPEFIKMNPIGKVPVLETP 60
           M L I+A     +S +A++     GV  + +    M    + PE++ + P GK+PVL   
Sbjct: 1   MVLTIYA-PLFASSKRAVVTLVEKGVSFETVNVDLMKGEQRQPEYLAIQPFGKIPVLVDG 60

Query: 61  DGPIFESNAIARYVA-RLKADSP-LFGSSSIDYGHIEQWIDFASLEIDPNILTW-----Y 120
           D  IFES AI RY+A + ++  P L G +  + G +EQW+D  +    P +L       +
Sbjct: 61  DYKIFESRAIMRYIAEKYRSQGPDLLGKTIEERGQVEQWLDVEATSYHPPLLALTLNIVF 120

Query: 121 RPRIGRAAYLPPVEEAAIAALKRGLGALNTHLASNTYLVGHFVTLADI 162
            P +G  A    ++E+    L   L      L+ N YL G FV+LAD+
Sbjct: 121 APLMGFPADEKVIKESE-EKLAEVLDVYEAQLSKNEYLAGDFVSLADL 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FUM17.4e-20783.02Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1[more]
Q6YW463.8e-19580.14Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022... [more]
Q9ZRI71.1e-18978.67Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022... [more]
Q5Z6279.1e-18977.01Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g057... [more]
Q9FVT21.9e-17874.00Probable elongation factor 1-gamma 2 OS=Arabidopsis thaliana OX=3702 GN=At1g5772... [more]
Match NameE-valueIdentityDescription
XP_023530487.17.85e-308100.00elongation factor 1-gamma-like [Cucurbita pepo subsp. pepo] >XP_023530496.1 elon... [more]
KAG7032457.11.85e-30699.53hypothetical protein SDJN02_06505 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022929968.15.08e-30498.82elongation factor 1-gamma-like isoform X3 [Cucurbita moschata][more]
XP_022929967.13.01e-30398.82elongation factor 1-gamma-like isoform X2 [Cucurbita moschata][more]
XP_022929966.13.77e-30398.82elongation factor 1-gamma-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1ETQ12.46e-30498.82elongation factor 1-gamma-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1EP331.46e-30398.82elongation factor 1-gamma-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1EPL41.83e-30398.82elongation factor 1-gamma-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JYI52.02e-30398.58elongation factor 1-gamma-like OS=Cucurbita maxima OX=3661 GN=LOC111490019 PE=4 ... [more]
A0A6J1DXR11.37e-28592.65elongation factor 1-gamma-like OS=Momordica charantia OX=3673 GN=LOC111025489 PE... [more]
Match NameE-valueIdentityDescription
AT1G57720.11.3e-17974.00Translation elongation factor EF1B, gamma chain [more]
AT1G57720.21.3e-17974.00Translation elongation factor EF1B, gamma chain [more]
AT1G09640.11.8e-17673.22Translation elongation factor EF1B, gamma chain [more]
AT1G09640.21.2e-9246.45Translation elongation factor EF1B, gamma chain [more]
AT2G30870.11.5e-1331.55glutathione S-transferase PHI 10 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001662Elongation factor 1B gamma, C-terminalSMARTSM01183EF1G_2coord: 261..369
e-value: 1.0E-66
score: 237.7
IPR001662Elongation factor 1B gamma, C-terminalPFAMPF00647EF1Gcoord: 261..369
e-value: 3.0E-41
score: 139.7
IPR001662Elongation factor 1B gamma, C-terminalPROSITEPS50040EF1G_Ccoord: 262..422
score: 79.404572
IPR004046Glutathione S-transferase, C-terminalPFAMPF00043GST_Ccoord: 128..197
e-value: 2.5E-8
score: 34.0
IPR036433Elongation factor EF1B gamma, C-terminal domain superfamilyGENE3D3.30.70.1010Translation elongation factor EF1B, gamma chain, conserved domaincoord: 262..422
e-value: 1.8E-69
score: 234.5
IPR036433Elongation factor EF1B gamma, C-terminal domain superfamilySUPERFAMILY89942eEF1-gamma domaincoord: 262..422
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 2..74
e-value: 6.3E-21
score: 76.4
NoneNo IPR availableGENE3D1.20.1050.10coord: 80..217
e-value: 6.2E-39
score: 135.0
NoneNo IPR availableSFLDSFLDG00358Main_(cytGST)coord: 6..190
e-value: 5.6E-30
score: 99.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 221..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..269
NoneNo IPR availablePANTHERPTHR44372:SF5SUBFAMILY NOT NAMEDcoord: 1..422
NoneNo IPR availableCDDcd03181GST_C_EF1Bgamma_likecoord: 90..210
e-value: 3.12605E-52
score: 169.279
NoneNo IPR availableCDDcd03044GST_N_EF1Bgammacoord: 3..77
e-value: 4.1935E-27
score: 101.177
IPR004045Glutathione S-transferase, N-terminalPFAMPF02798GST_Ncoord: 11..76
e-value: 2.2E-15
score: 56.7
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 1..82
score: 19.540607
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 6..190
e-value: 5.6E-30
score: 99.2
IPR044628Elongation factor 1-gamma, plantPANTHERPTHR44372ELONGATION FACTOR 1-GAMMA 1-RELATEDcoord: 1..422
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 87..215
score: 21.758574
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 73..214
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 3..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g16740.1Cp4.1LG01g16740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0006414 translational elongation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003746 translation elongation factor activity