Cp4.1LG04g01870 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g01870
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAsparagine--tRNA ligase
LocationCp4.1LG04: 258791 .. 261885 (+)
RNA-Seq ExpressionCp4.1LG04g01870
SyntenyCp4.1LG04g01870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAACTGAAAAGACCGGTCAGTGTTAATATGTAGAGACGGAACTTGAGATATCAAATGTGGTTTAGGGTTTTGAATTCAACTGCAGTGAAGATACACCGGGAACGGACCAATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAGTCTCTCAAAGCTGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCACCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATGTCGTTCATCTCCGCCCTAGAACAAACACGGTAAAAGAAATTTTAATCGTTTGTCAAATTAAATTTGAAGTTAGTTATTGGATCGAATCTTTTCGATTGATCTTTTCACATTCTGCATTTCATTTCTTATGTAATTATATAACTTGGCATTTACATGGTCTGATCCTCTCTAACGTCAATAGAAAGTTGCAATTTATAGAACATGATCACTTTTATTGATGAGTATGTGTTTTCTTCATTTGACGATTACAGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTTAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAGAACCCACCTCCATCAGAAGCTGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTTCAAACCTGAAGGCTTCTAAGGCAAGCAAGCAGGAAATCGGTGCTTCTGTTTTGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGGTTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCAGAGAAGGATGGGAAAGTTGATTATGGAAGAGATTTCTTCGCTCGGCAAGCGTTTTTGACTGTTTCGGGCCAGTTGCAAGTCGAAACTTATGCGTGTGCACTCAGTAGTGTATACACCTTTGGCCCAACCTTCCGTGCCGAAAATTCTCACACCTCGAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTGTTACCATGCCAAAATTGTATTCTTAATTGTATGTTTTTCTGCATACTTGCATATAACCATCTTAAGACAATTCTGGCACTTAGATTATAGTTGAGTTTATGTGTTCTAGGATGAGTTTATCATACTCATTGATGTGAAAAAACTAAGTTCTTTTATAATAAATTTAGGATCCTACTTGATATACTTTCCATGAGAACTGTAATTGAGAAGAAACTGGTCGAGGACTAAATGTCTGTCTCTCTGTGCTACTAAGCAATATGGCAAACAACAATGGATATCTATTAGGAATAAGCAATCAATGGAACTCTACAGTAGTAGTTCAGTGCTTTTCTTCTAGAGATTTGATTGAATTTACCATATTTGACATATTTTCTTTAATTGGTTGAAGGATGATATGAATTGCGCAGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGACGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCGACCCCTTTTGTACGAACTTCGTATACAGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCTGAACATGAAAGGTCTGTAAACTCCCATGCTTTTCATTTACGTTCCACTCGAACAATGGCGATAGTTCTCATTGTTTTTCTCTTGCTCAGATACTTGACTGAGGTAGTATTTAAGAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTAGTTCACTTCGTTTCGTTTTTACTTCTATAACTGTGATTAGTTTCACCCTTTTAATCCCTTTGTTTGTTGAAAATCAAAACTTTTGCCTTGGATTGAAGGTTGGGGAATTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGTGACAGGTCGAAGAACAATTCATACTTCTCTTGTTGATTCTTGTTTCTTTTGTGAACTATGAAGTCTGAATTATCATCTCCTGCAGGATTAAGGAGGTGGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTCGGTCTTGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCCAGATATCCAGGACGAGCGGACCTATGATATGATATATTTCGTAACACAAAGAAGGTCTTAGATTTCTTTACTAGCTGATGAAACTAAACTCCTTTGAACCAAAGGAGATTTGATTGTTTGTATTATATTTTTGATCTTTTTAGTTGTTGGGTTTAATTTATTCTCATAATTCTTGGATTTGTGGGTTGATTTGAAATGACATTCATAGGTTTCTTCATGTTTTGGATGTTTTGGAACTAACATTTTTTAATTTGTTTAATCATCTAATGCATACTATAAGATGCTTCCAATTATGCTCCTTAAGTTGTCTTTAATGTTCTAAAAGTTTATTATATATATAATTTTGAGAAGAAAAAAAAGCAAGTCTAAATTAATAAAAATATTTTTGAACCTTAAATAAATATAATTTTAATATTAA

mRNA sequence

CCAAACTGAAAAGACCGGTCAGTGTTAATATGTAGAGACGGAACTTGAGATATCAAATGTGGTTTAGGGTTTTGAATTCAACTGCAGTGAAGATACACCGGGAACGGACCAATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAGTCTCTCAAAGCTGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCACCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATGTCGTTCATCTCCGCCCTAGAACAAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTTAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAGAACCCACCTCCATCAGAAGCTGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTTCAAACCTGAAGGCTTCTAAGGCAAGCAAGCAGGAAATCGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGCGCAGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGACGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCGACCCCTTTTGTACGAACTTCGTATACAGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCTGAACATGAAAGATACTTGACTGAGGTAGTATTTAAGAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAATTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGTGACAGGATTAAGGAGGTGGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTCGGTCTTGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCCAGATATCCAGGACGAGCGGACCTATGATATGATATATTTCGTAACACAAAGAAGGTCTTAGATTTCTTTACTAGCTGATGAAACTAAACTCCTTTGAACCAAAGGAGATTTGATTGTTTGTATTATATTTTTGATCTTTTTAGTTGTTGGGTTTAATTTATTCTCATAATTCTTGGATTTGTGGGTTGATTTGAAATGACATTCATAGGTTTCTTCATGTTTTGGATGTTTTGGAACTAACATTTTTTAATTTGTTTAATCATCTAATGCATACTATAAGATGCTTCCAATTATGCTCCTTAAGTTGTCTTTAATGTTCTAAAAGTTTATTATATATATAATTTTGAGAAGAAAAAAAAGCAAGTCTAAATTAATAAAAATATTTTTGAACCTTAAATAAATATAATTTTAATATTAA

Coding sequence (CDS)

ATGGCAGAGCATTCCGCGCCACCCATTGACCAACTATCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAGTCTCTCAAAGCTGAATTCTCCGATCGCGTTCCGATCCGTACAATCGTCTGCCGGCCAGACGGCGGCTCTGGACTCGCTGGCCATAAGGTTCGGGTTGGTGGTTGGGTTAAAACCGGAAGGAAGGCGGACAAGGACGCTTTTGCCTTTCTTGAGTTGAACGACGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTCCCCACCGGAACCTGCGTTGTTGTCGATGGCGTTTTTAAGCTTCCTCCACCGGATAAGCACCAGAAAATCGAGCTTCGTGCTGAGGCTGTCGTCCATGTTGGTCCGGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAGCTCACTCTTGAGTTTCTTAGGGATGTCGTTCATCTCCGCCCTAGAACAAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCACACATTTTTTAACAAGCATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGAGCGGGTGAAATGTTTCAAGTTACCACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAGAACCCACCTCCATCAGAAGCTGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTTCAAACCTGAAGGCTTCTAAGGCAAGCAAGCAGGAAATCGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGCGCAGAAGCATATGTGAGATTTCTGTGTCAATGGTTACTTGATAACTGCCTAGACGACATGAAATTTATGGCTGATAAGTACGACCGAACTTGTATCGATCGTCTCAAATTGGTCTCTTCGACCCCTTTTGTACGAACTTCGTATACAGAAGCTATTGAAATACTTGAGGAAGCCATAAAGAAGGGTAAGGAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTAGCTTCTGAACATGAAAGATACTTGACTGAGGTAGTATTTAAGAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAATTGATTGGTGGAAGTCAAAGGGAGGAGCGATTCGAGGTTATTCGTGACAGGATTAAGGAGGTGGGTCTTCCACTTGAACCATATGAATGGTATCTTGATCTGAGACGATACGGAACAGTCAAGCACAGCGGTTTCGGTCTTGGCTTCGAGCGAATGCTTCTTTTTGCCACAGGCATTGACAACATCCGAGACGTTATCCCCTTCCCCAGATATCCAGGACGAGCGGACCTATGA

Protein sequence

MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Homology
BLAST of Cp4.1LG04g01870 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 4.2e-198
Identity = 351/574 (61.15%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRDV+HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEI---------------------------------- 300
            AR IV E+G  V+ LKA+KASK+ I                                  
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 --------------------------------------------------EFWMVEPEIA 360
                                                             EFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 489
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

BLAST of Cp4.1LG04g01870 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.5e-190
Identity = 347/576 (60.24%), Postives = 410/576 (71.18%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRDV+HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEI---------------------------------- 300
            AR IVKE+GEAV+ LK +KASK+EI                                  
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 --------------------------------------------------EFWMVEPEIA 360
                                                             EFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 489
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

BLAST of Cp4.1LG04g01870 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.4e-121
Identity = 236/465 (50.75%), Postives = 305/465 (65.59%), Query Frame = 0

Query: 50  LAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIV---PTGTC 109
           L GH + + GWV+T R  ++  F F+E+NDGS   N Q+I    +A   Q++    TG  
Sbjct: 18  LIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVS 77

Query: 110 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNT 169
           V   G   +  P K Q +E++A A+  +G  DP  Y L K + T EFLR + HLRPRTNT
Sbjct: 78  VSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNT 137

Query: 170 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 229
           I AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MFQVTTL           
Sbjct: 138 IGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQVTTLDQ--------- 197

Query: 230 IKNPPPS-EAEVDEARKIVKE----------KGE----AVSNL---------KASKASKQ 289
             NP  + E  VD  +    +           GE    A+S++         + S  S+ 
Sbjct: 198 -NNPARTPEGRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTFGPTFRAENSNTSRH 257

Query: 290 EIEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 349
             EFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F         I RL+ V
Sbjct: 258 LAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFNKHVATDLISRLEHV 317

Query: 350 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 409
            +T F R SYT A+ ILE+A KK   FE  V+WG+DL SEHER+L E  F KPVI+ +YP
Sbjct: 318 INTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERFLAEEFFGKPVILTDYP 377

Query: 410 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 469
           K IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+  ++KE  LP E Y WY
Sbjct: 378 KDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWY 437

Query: 470 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 488
           L+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+AD
Sbjct: 438 LELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKAD 466

BLAST of Cp4.1LG04g01870 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.6e-120
Identity = 221/464 (47.63%), Postives = 294/464 (63.36%), Query Frame = 0

Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR++ HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL  ++       
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTLNPDS------- 196

Query: 229 IKNPPPSEAEVDEARKI------VKEKGEAVSNLKA-----------------SKASKQE 288
              P   E +VD  +        +   G+    + A                 S   +  
Sbjct: 197 --LPRTEEGQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRHL 256

Query: 289 IEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVS 348
            EFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V 
Sbjct: 257 AEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFVL 316

Query: 349 STPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPK 408
             PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YPK
Sbjct: 317 EKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYPK 376

Query: 409 GIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYL 468
            IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WYL
Sbjct: 377 EIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWYL 436

Query: 469 DLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 488
           D RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 437 DSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

BLAST of Cp4.1LG04g01870 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.0e-119
Identity = 220/464 (47.41%), Postives = 293/464 (63.15%), Query Frame = 0

Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR++ HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL  ++       
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTLNPDS------- 196

Query: 229 IKNPPPSEAEVDEARKI------VKEKGEAVSNLKA-----------------SKASKQE 288
              P   E +VD  +        +   G+    + A                 S   +  
Sbjct: 197 --LPRTEEGQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRHL 256

Query: 289 IEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVS 348
            EFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V 
Sbjct: 257 AEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFVL 316

Query: 349 STPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPK 408
             PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YPK
Sbjct: 317 EKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYPK 376

Query: 409 GIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYL 468
            IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WYL
Sbjct: 377 EIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWYL 436

Query: 469 DLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 488
           D RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 437 DSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

BLAST of Cp4.1LG04g01870 vs. NCBI nr
Match: XP_023531470.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 488/572 (85.31%), Postives = 488/572 (85.31%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. NCBI nr
Match: XP_022932543.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 488/572 (85.31%), Postives = 488/572 (85.31%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. NCBI nr
Match: KAG6588323.1 (Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020885.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937 bits (2421), Expect = 0.0
Identity = 486/572 (84.97%), Postives = 486/572 (84.97%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. NCBI nr
Match: XP_022965912.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 485/572 (84.79%), Postives = 485/572 (84.79%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAV LNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVG VDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 460/572 (80.42%), Postives = 477/572 (83.39%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDDA TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           R+IVKEKGEAV+ LKASKASK+EI                                    
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. ExPASy TrEMBL
Match: A0A6J1F212 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1)

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 488/572 (85.31%), Postives = 488/572 (85.31%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. ExPASy TrEMBL
Match: A0A6J1HLL2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 485/572 (84.79%), Postives = 485/572 (84.79%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAV LNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVG VDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           RKIVKEKGEAVSNLKASKASKQEI                                    
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 460/572 (80.42%), Postives = 477/572 (83.39%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDDA TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           R+IVKEKGEAV+ LKASKASK+EI                                    
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 895 bits (2313), Expect = 0.0
Identity = 461/572 (80.59%), Postives = 476/572 (83.22%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDDA TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           R+IVKEKGEAV+ LKASKASK+EI                                    
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKEV LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

BLAST of Cp4.1LG04g01870 vs. ExPASy TrEMBL
Match: A0A5D3CE86 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003180 PE=3 SV=1)

HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 457/572 (79.90%), Postives = 477/572 (83.39%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LN+DAS Y+SLKAEFSDRVPIRTIV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA+VADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEI------------------------------------ 300
           R IVKEKGEAV++LKA+KASK+EI                                    
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 ------------------------------------------------EFWMVEPEIAFA 360
                                                           EFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRF+CQWLLDNCLDDMKFMA+KYDRTCIDRLK+VSSTPFVR SYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+KKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 488
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

BLAST of Cp4.1LG04g01870 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.0e-199
Identity = 351/574 (61.15%), Postives = 419/574 (73.00%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRDV+HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEI---------------------------------- 300
            AR IV E+G  V+ LKA+KASK+ I                                  
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 --------------------------------------------------EFWMVEPEIA 360
                                                             EFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 489
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

BLAST of Cp4.1LG04g01870 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 666.4 bits (1718), Expect = 1.8e-191
Identity = 347/576 (60.24%), Postives = 410/576 (71.18%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRDV+HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEI---------------------------------- 300
            AR IVKE+GEAV+ LK +KASK+EI                                  
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 --------------------------------------------------EFWMVEPEIA 360
                                                             EFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 489
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

BLAST of Cp4.1LG04g01870 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 7.1e-116
Identity = 230/493 (46.65%), Postives = 311/493 (63.08%), Query Frame = 0

Query: 24  YKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    G  + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEAAVADLGQI----VPTGTCVVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDP 143
            NLQ ++ +      Q+    + TG  V V G        K QK+EL+ E ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQGTK-QKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLL-SEAERLEKELIKNPPPSEAEVDEARKIVKE----------KG 263
            SDCEGAGE F VTTL+ S  E  +  +   P      +D ++    +           G
Sbjct: 261 ASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNG 320

Query: 264 E----AVSNL---------KASKASKQEIEFWMVEPEIAFADLQDDMNCAEAYVRFLCQW 323
           E    A+S++         + S  S+   EFWM+EPE+AFADL DDM CA AY++++ ++
Sbjct: 321 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 380

Query: 324 LLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEW 383
           +LDNC +DM+F     ++  I RL  V+   F++  YT+AIEIL   +K  K+F+  V+W
Sbjct: 381 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEIL---LKANKKFDFPVKW 440

Query: 384 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 443
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 441 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 500

Query: 444 GSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 488
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 501 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 560

BLAST of Cp4.1LG04g01870 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 375.6 bits (963), Expect = 6.2e-104
Identity = 225/633 (35.55%), Postives = 327/633 (51.66%), Query Frame = 0

Query: 18  NDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDA------ 77
           ++D S      +++S RV ++T++ R D G+GLAG +V +GGWVK+ R   K++      
Sbjct: 13  DNDLSPKPITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 72

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 73  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 132

Query: 138 -------FAFLELNDGSCPQNLQVIIEAAVADL--GQIVPTGTCVVVDGVFKLP-PPDKH 197
                  F FL ++DGS   +LQV++++A++ +   Q++  GTC+V +GV +LP      
Sbjct: 133 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 192

Query: 198 QKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYAT 257
             IEL AE ++HVG VDP +YPL K +L L  LRD  H RPRT T+ +V R+ +AL  A+
Sbjct: 193 HVIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLAS 252

Query: 258 HTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAE---VD 317
           HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +D
Sbjct: 253 HTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSID 312

Query: 318 EARKIVKEKGEAVSNLKASKASKQEI---------------------------------- 377
             + ++KEK   + +LK S ++++ +                                  
Sbjct: 313 TVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKL 372

Query: 378 --------------------------------------------------EFWMVEPEIA 437
                                                             E W VE E+A
Sbjct: 373 DFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMA 432

Query: 438 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 488
           FA+L D M+CA+ Y +FLC+++L+N  +DMKF++ + D+T   RL+  +S+  +R SYTE
Sbjct: 433 FAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTE 492

BLAST of Cp4.1LG04g01870 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 7.3e-20
Identity = 87/330 (26.36%), Postives = 157/330 (47.58%), Query Frame = 0

Query: 163 RTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQV-----TTLLS 222
           RT    A+ RI+  +  A   +    GFL IHTP +     EG   +F++        L+
Sbjct: 219 RTPANQAIFRIQCQVQIAFREYLQSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLA 278

Query: 223 EAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIEFWMVEPEIAF-A 282
           ++ +L K++        A   + R++  E G  V   + S   +   EF  ++ E+    
Sbjct: 279 QSPQLHKQM--------AICGDMRRVF-EVG-PVFRAEDSFTHRHLCEFVGLDVEMEIRM 338

Query: 283 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 342
              + M+       F+   + + C  +++ +  +Y       LK +  T  +R ++ E I
Sbjct: 339 HYSEIMDLVGELFPFIFTKIEERCPKELESVRKQYP---FQSLKFLPQT--LRLTFAEGI 398

Query: 343 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKP----VIVYNYPKGIKAFY-MR 402
           ++L+EA   G+E    V+   DL +E ER L ++V +K      +++ YP  ++ FY M 
Sbjct: 399 QMLKEA---GEE----VDPLGDLNTESERKLGQLVLEKYKTEFYMLHRYPSAVRPFYTMP 458

Query: 403 LNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTV 462
             +DS    + DV + +  E++ G+QR    E++  R +E G+ ++    Y+D  RYG  
Sbjct: 459 YENDSNYSNSFDVFI-RGEEIMSGAQRIHDPELLEKRARECGIDVKTISTYIDAFRYGAP 518

Query: 463 KHSGFGLGFERMLLFATGIDNIRDVIPFPR 482
            H GFG+G ER+++    ++NIR    FPR
Sbjct: 519 PHGGFGVGLERVVMLLCALNNIRKTSLFPR 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SW964.2e-19861.15Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK12.5e-19060.24Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC91.4e-12150.75Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
Q7MVE53.6e-12047.63Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ... [more]
B2RK421.0e-11947.41Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207... [more]
Match NameE-valueIdentityDescription
XP_023531470.10.085.31asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo][more]
XP_022932543.10.085.31asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata][more]
KAG6588323.10.084.97Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022965912.10.084.79asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima][more]
XP_022961000.10.080.42asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A... [more]
Match NameE-valueIdentityDescription
A0A6J1F2120.085.31Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1[more]
A0A6J1HLL20.084.79Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1[more]
A0A6J1HAP90.080.42Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1[more]
A0A6J1JKK10.080.59Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1[more]
A0A5D3CE860.079.90Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G56680.13.0e-19961.15Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.11.8e-19160.24Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.17.1e-11646.65Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT3G07420.16.2e-10435.55asparaginyl-tRNA synthetase 2 [more]
AT4G26870.17.3e-2026.36Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 403..419
score: 44.79
coord: 216..228
score: 25.7
coord: 443..457
score: 47.27
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 153..214
e-value: 8.7E-12
score: 44.7
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 258..481
e-value: 2.2E-46
score: 158.4
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 143..487
e-value: 2.3E-85
score: 288.9
NoneNo IPR availableGENE3D2.40.50.140coord: 34..138
e-value: 3.5E-9
score: 38.4
NoneNo IPR availablePANTHERPTHR22594:SF52BNAC03G13340D PROTEINcoord: 256..488
coord: 1..230
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 256..488
coord: 1..230
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 54..135
e-value: 2.74549E-25
score: 97.2508
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 132..487
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 172..478
score: 11.927794
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 48..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g01870.1Cp4.1LG04g01870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding