CsGy6G012180 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G012180
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionGDSL esterase/lipase
LocationGy14Chr6: 10587984 .. 10589555 (-)
RNA-Seq ExpressionCsGy6G012180
SyntenyCsGy6G012180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACTACTTGTTTATCTATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGTCAGCCCCTGGTTCCAGCATTGTTCACATTCGGGGACTCTGTCCTCGATGTTGGGATCAATAATCATCTTAAAACCCTCATTAAAGCAAACTTCCTCCCTTACGGAAGAGATTTTATAACTCACAAGCCTACGGGAAGATTCTGCAATGGGAAACTTGCCTCAGATTTCACTGGTACTTTGCACCTAGATTTCATTTCACTAACTTATGTTCATAAATAATCAACACATGATCTAATTTCTTTTGTTGCTTATGAATAGCTGAGTACCTAGGATTTACATCTTACCCACAAGCTTACCTCGGTGGAGGAGGAAAGGACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCTTCTGGATACCTAGACACAACTGCAGAATTATATGTAATAAATCAAAAACTTCAGTTTTTCTATCTTGGGCACTGCCTTAAAATAACATGTTTAGGCTTGTGGTGTTGTAGAATGCACTTTCCTTTACTCAGCAGCTGGAACACTACAAGGAGTATCAGAACAAAGTAGCTGAGGTTGCTGGAAAATCCAATGCTTCATCTATTATATCTGGTGCCATTTACCTTGTCAGTGCTGGGAGCAATGACTTTCTGCAAAACTATTACATCAATCCTCTACTTTACAAGAAATATACAGTTAGTCAGTTTAGCGAAATCATCATCACCTCCTACATAATTTTCATTCAGGTCTGGCTCTCCATCAATTCCTAAATTGATTCAATTAAAAAGGTGCAACAGATACATATATGAATGTTGTGTAATCAAAATATATGATTTCAGAATCTATATGCATTGGGAGCACGGAGAATTGGAGTAACAACATTGCCACCGCTTGGATGTCTCCCAGCAGCCATAACAGTTTTTGGATCTGATAGCAACGAGTGTGTGGCAAAGCTAAACAATGATGCAGTTGCGTTCAATAGCAAGCTCAATGCCACATCTCAGAGCTTACGAACCAAGCTTTATGGCCTCAATCTGGTTGTCTTGGACTCTTACAAACCTCTCTATGACCTTATCACAAAGCCCGCTGAACATGGTATGCCATTGAGATTTTTTAGAACACAAGGACACGTTGTATTAACCCTTGTTCATGTAAGGTCACGGTTTGCCATTAAGAGTAGTTCAGAGTAAATAAAAATGGCCTGCATCAACTCCAACCTAGGATCTCCATACATATAATATCTTGGATAAAGTAGAATGTAAATCTCATGAAATATCTCACATGGAATCACAAAGGACTGAAAAATCCACATTGATACATCAGAACCCACGATGTGTCACTAACTCATGCATAAAATTACGTAGGTTTCTCTGAGGCAAGGAAGGCCTGCTGTGGCACCGGCTTGCTTGAAACATCATTCCTATGCAATACAGAATCGGTAGGGACATGTGCTAATGCATCTCAATACGTGTTCTGGGATGGATTCCACCCTTCAGAAGCTGCAAACAAGTTCTTAGCTAGTAGTCTGCTAGCCAGTGGCATCTCTCTCATTTCATAA

mRNA sequence

ATGGATACTACTTGTTTATCTATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGTCAGCCCCTGGTTCCAGCATTGTTCACATTCGGGGACTCTGTCCTCGATGTTGGGATCAATAATCATCTTAAAACCCTCATTAAAGCAAACTTCCTCCCTTACGGAAGAGATTTTATAACTCACAAGCCTACGGGAAGATTCTGCAATGGGAAACTTGCCTCAGATTTCACTGCTGAGTACCTAGGATTTACATCTTACCCACAAGCTTACCTCGGTGGAGGAGGAAAGGACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCTTCTGGATACCTAGACACAACTGCAGAATTATATAATGCACTTTCCTTTACTCAGCAGCTGGAACACTACAAGGAGTATCAGAACAAAGTAGCTGAGGTTGCTGGAAAATCCAATGCTTCATCTATTATATCTGGTGCCATTTACCTTGTCAGTGCTGGGAGCAATGACTTTCTGCAAAACTATTACATCAATCCTCTACTTTACAAGAAATATACAGTTAGTCAGTTTAGCGAAATCATCATCACCTCCTACATAATTTTCATTCAGAATCTATATGCATTGGGAGCACGGAGAATTGGAGTAACAACATTGCCACCGCTTGGATGTCTCCCAGCAGCCATAACAGTTTTTGGATCTGATAGCAACGAGTGTGTGGCAAAGCTAAACAATGATGCAGTTGCGTTCAATAGCAAGCTCAATGCCACATCTCAGAGCTTACGAACCAAGCTTTATGGCCTCAATCTGGTTGTCTTGGACTCTTACAAACCTCTCTATGACCTTATCACAAAGCCCGCTGAACATGGTTTCTCTGAGGCAAGGAAGGCCTGCTGTGGCACCGGCTTGCTTGAAACATCATTCCTATGCAATACAGAATCGGTAGGGACATGTGCTAATGCATCTCAATACGTGTTCTGGGATGGATTCCACCCTTCAGAAGCTGCAAACAAGTTCTTAGCTAGTAGTCTGCTAGCCAGTGGCATCTCTCTCATTTCATAA

Coding sequence (CDS)

ATGGATACTACTTGTTTATCTATTTTCTTTCTTCTCCTTGCTCCTGTGATCTCTCTGGCAAATGGTCAGCCCCTGGTTCCAGCATTGTTCACATTCGGGGACTCTGTCCTCGATGTTGGGATCAATAATCATCTTAAAACCCTCATTAAAGCAAACTTCCTCCCTTACGGAAGAGATTTTATAACTCACAAGCCTACGGGAAGATTCTGCAATGGGAAACTTGCCTCAGATTTCACTGCTGAGTACCTAGGATTTACATCTTACCCACAAGCTTACCTCGGTGGAGGAGGAAAGGACCTCCTGATTGGAGCAAGCTTTGCCTCAGCTGCTTCTGGATACCTAGACACAACTGCAGAATTATATAATGCACTTTCCTTTACTCAGCAGCTGGAACACTACAAGGAGTATCAGAACAAAGTAGCTGAGGTTGCTGGAAAATCCAATGCTTCATCTATTATATCTGGTGCCATTTACCTTGTCAGTGCTGGGAGCAATGACTTTCTGCAAAACTATTACATCAATCCTCTACTTTACAAGAAATATACAGTTAGTCAGTTTAGCGAAATCATCATCACCTCCTACATAATTTTCATTCAGAATCTATATGCATTGGGAGCACGGAGAATTGGAGTAACAACATTGCCACCGCTTGGATGTCTCCCAGCAGCCATAACAGTTTTTGGATCTGATAGCAACGAGTGTGTGGCAAAGCTAAACAATGATGCAGTTGCGTTCAATAGCAAGCTCAATGCCACATCTCAGAGCTTACGAACCAAGCTTTATGGCCTCAATCTGGTTGTCTTGGACTCTTACAAACCTCTCTATGACCTTATCACAAAGCCCGCTGAACATGGTTTCTCTGAGGCAAGGAAGGCCTGCTGTGGCACCGGCTTGCTTGAAACATCATTCCTATGCAATACAGAATCGGTAGGGACATGTGCTAATGCATCTCAATACGTGTTCTGGGATGGATTCCACCCTTCAGAAGCTGCAAACAAGTTCTTAGCTAGTAGTCTGCTAGCCAGTGGCATCTCTCTCATTTCATAA

Protein sequence

MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS*
Homology
BLAST of CsGy6G012180 vs. ExPASy Swiss-Prot
Match: Q9FFC6 (GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3 SV=3)

HSP 1 Score: 471.1 bits (1211), Expect = 1.1e-131
Identity = 234/338 (69.23%), Postives = 277/338 (81.95%), Query Frame = 0

Query: 16  VISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKPTGRFCNGKLA 75
           V+ +   QPLVPA+F FGDSV+DVG NN + T++KANF PYGRDF TH PTGRFCNGKLA
Sbjct: 25  VMVVMKAQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLA 84

Query: 76  SDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNALSFTQQLEHY 135
           +DFTAE LGF SYPQAYL     GK+LLIGA+FASAASGY D TA+LY+A+S  QQLEHY
Sbjct: 85  TDFTAENLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHY 144

Query: 136 KEYQNKVAEVA---GKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTVSQFSEII 195
           K+Y +++ E+A     SNAS+IIS  IY+VSAGS+DF+QNYYINPLLY+  +  +FS+++
Sbjct: 145 KDYISRIQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLL 204

Query: 196 ITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNNDAVAFNSKLN 255
           I SY  FIQNLY+LGARRIGVTTLPPLGCLPAAITV G     C  KLNNDA++FN+KLN
Sbjct: 205 ILSYSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLN 264

Query: 256 ATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLETSFLCNTESV 315
            TSQ L+  L GLNLVV D Y+PLYDL T+P+E GF+EAR+ACCGTGLLETS LCN +SV
Sbjct: 265 TTSQDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSV 324

Query: 316 GTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           GTC NA++YVFWDGFHP+EAANK LA +LL SGISLIS
Sbjct: 325 GTCNNATEYVFWDGFHPTEAANKILADNLLVSGISLIS 362

BLAST of CsGy6G012180 vs. ExPASy Swiss-Prot
Match: Q9FFN0 (GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.7e-117
Identity = 214/347 (61.67%), Postives = 261/347 (75.22%), Query Frame = 0

Query: 5   CLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHK 64
           CLS+     A V     G+ LVPAL   GDSV+D G NNH  TL+KANF PYGRDF+ H 
Sbjct: 10  CLSVIACFYAGV---GTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHS 69

Query: 65  PTGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYN 124
            TGRF NGKLA+DFTAE LGFTSYP AYL       +LL GA+FAS ASG+ D TA  YN
Sbjct: 70  ATGRFSNGKLATDFTAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYN 129

Query: 125 ALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYT 184
           A++ +QQL++YKEYQNKV  + GK  A+ I SGAI+L+S GS+DFLQ+YYINP+L + +T
Sbjct: 130 AITLSQQLKNYKEYQNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFT 189

Query: 185 VSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGS-DSNECVAKLNND 244
             Q+S+ ++ SY  F+QNLY LGARRIGVTTLPPLGCLPAAIT+FG   +N CV +LN D
Sbjct: 190 PDQYSDHLLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQD 249

Query: 245 AVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLET 304
           AV+FN+KLN TS +L   L GL LVV D Y PL +++  P E+GF E+R+ACCGTG +ET
Sbjct: 250 AVSFNTKLNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMET 309

Query: 305 SFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           SFLCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A++LL  GI LIS
Sbjct: 310 SFLCNALSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 353

BLAST of CsGy6G012180 vs. ExPASy Swiss-Prot
Match: Q9LZC5 (GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 5.0e-116
Identity = 211/347 (60.81%), Postives = 258/347 (74.35%), Query Frame = 0

Query: 6   LSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKP 65
           L  F ++      +  G+PLVPAL   GDSV+D G NN L TLIKANF PYGRDF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 66  TGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNA 125
           TGRF NGKLA+DFTAE LGFTSYP  YL     G +LL GA+FAS ASGY D TA  YNA
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 126 LSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTV 185
           ++  QQL++YKEYQNKV  + G   A+ I SGAI+L+S GS+DFLQ+YYINP+L + +T 
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 186 SQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFG--SDSNECVAKLNND 245
            Q+S+ ++  Y  F+QNLY LGAR+IGVTTLPPLGCLPAAIT+FG   ++N CV +LN D
Sbjct: 188 DQYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQD 247

Query: 246 AVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLET 305
           AV+FN+KLN TS +L   L GL LVV D Y PL ++   P E+GF E+R+ACCGTG +ET
Sbjct: 248 AVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVET 307

Query: 306 SFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           SFLCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A++LL  GI LIS
Sbjct: 308 SFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 354

BLAST of CsGy6G012180 vs. ExPASy Swiss-Prot
Match: Q0WPI9 (GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.2e-109
Identity = 193/340 (56.76%), Postives = 255/340 (75.00%), Query Frame = 0

Query: 11  LLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKPTGRFC 70
           LLL       +   +VPAL  FGDS++DVG NN+L +++K+NFLPYGRDFI  +PTGRFC
Sbjct: 12  LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFC 71

Query: 71  NGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNALSFTQ 130
           NGKLA DF+AEYLGF+SYP A+L      +++LIGA+FASA+SGY D T+  + ++S T+
Sbjct: 72  NGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTR 131

Query: 131 QLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTVSQFSE 190
           QL +Y+ YQN+V  + G+ NA  + S  I+++SAGS+DFLQNYYINPLL    T  QF++
Sbjct: 132 QLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFAD 191

Query: 191 IIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNNDAVAFNSK 250
           I++ S+  FIQNLY LGARRIGV +LPP+GCLPAAIT+FG+ +  CV +LNNDA+ FN+K
Sbjct: 192 ILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTK 251

Query: 251 LNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLETSFLCNTE 310
           L  T++ L  +  GL LV  + Y+P  D+IT P ++GF E ++ACCGTG +ETSFLCN+ 
Sbjct: 252 LENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSL 311

Query: 311 SVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           S GTC NA+ YVFWDGFHP+EA N+ LA  LL  GISLI+
Sbjct: 312 SFGTCVNATGYVFWDGFHPTEAVNELLAGQLLGQGISLIN 351

BLAST of CsGy6G012180 vs. ExPASy Swiss-Prot
Match: Q9LU14 (GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-108
Identity = 200/352 (56.82%), Postives = 255/352 (72.44%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISL--ANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGR 60
           MD    S   L L   +S+   +   LVPA+ TFGDSV+DVG NN+L TL +A++ PYGR
Sbjct: 1   MDRCTSSFLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGR 60

Query: 61  DFITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDT 120
           DF  HK TGRFCNGKLA+D TAE LGFT YP AYL     GK+LLIGA+FASAASGY D 
Sbjct: 61  DFANHKATGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDK 120

Query: 121 TAELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPL 180
            A L +A+   QQ+E++KEY++K+ ++AG   A SII GAI L+SAGS+DF+QNYY+NPL
Sbjct: 121 AALLNHAIPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPL 180

Query: 181 LYKKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVA 240
           LYK YTV  +   +I ++  FI+ +YA+GAR+IGVT+LPP GCLPAA T+FG     CV+
Sbjct: 181 LYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVS 240

Query: 241 KLNNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGT 300
           +LN DA  FN KLNA +  L+ +   L +VV D Y PLYDL+  P++ GF+EA K CCGT
Sbjct: 241 RLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGT 300

Query: 301 GLLE-TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLI 348
           G +E TS LCN +S GTC+NA+QYVFWD  HPSEAAN+ LA++L+  G SL+
Sbjct: 301 GTVETTSLLCNPKSFGTCSNATQYVFWDSVHPSEAANEILATALIGQGFSLL 352

BLAST of CsGy6G012180 vs. NCBI nr
Match: XP_004145774.1 (GDSL esterase/lipase At5g22810 [Cucumis sativus] >KGN46905.1 hypothetical protein Csa_020964 [Cucumis sativus])

HSP 1 Score: 684 bits (1766), Expect = 3.90e-248
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
           ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL
Sbjct: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN
Sbjct: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE
Sbjct: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS
Sbjct: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of CsGy6G012180 vs. NCBI nr
Match: XP_008458651.1 (PREDICTED: GDSL esterase/lipase At5g22810 [Cucumis melo] >KAA0033359.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYJ96643.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 667 bits (1720), Expect = 4.01e-241
Identity = 338/348 (97.13%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDTTCLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKD L+GASFASAASGYLDTTAEL
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDFLVGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALSFTQQLE+YKEYQNKVAEVAGK NASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YTVSQFS+IIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECV KLNN
Sbjct: 181 YTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAVAFNSKLNATSQSLRTKL GLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE
Sbjct: 241 DAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TSFLCNTESVGTCANAS+YVFWDGFHPSEAANKFLASSLLASGISLIS
Sbjct: 301 TSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of CsGy6G012180 vs. NCBI nr
Match: XP_038901478.1 (GDSL esterase/lipase At5g22810 [Benincasa hispida])

HSP 1 Score: 629 bits (1623), Expect = 2.26e-226
Identity = 322/348 (92.53%), Postives = 335/348 (96.26%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDT CLSIFFLL APVI+LANGQPLVPALFTFGDSVLDVGINNHLKT+IKA+FLPYGRDF
Sbjct: 1   MDTICLSIFFLL-APVITLANGQPLVPALFTFGDSVLDVGINNHLKTVIKADFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
           ITHKPTGRFCNGKLASDFTAEYLGFTSYPQ YL GG K+LLIGASFASAASGYLDTTAEL
Sbjct: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQPYLSGG-KNLLIGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALS TQQLE+YKEYQNKVAE+AGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSLTQQLEYYKEYQNKVAEIAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YT +QF  IIITSYI FIQNLYA+GARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN
Sbjct: 181 YTANQFGNIIITSYINFIQNLYAMGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAV+FNSKLNATSQSLRTKL GLNLVVLDSYKPLYDLITKPAE+GF E+RKACCGTGLLE
Sbjct: 241 DAVSFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAENGFFESRKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TS LCNTESVGTCANAS+YVFWDGFHPSEAANKFLAS+LLASGISLIS
Sbjct: 301 TSILCNTESVGTCANASEYVFWDGFHPSEAANKFLASNLLASGISLIS 346

BLAST of CsGy6G012180 vs. NCBI nr
Match: XP_022958433.1 (GDSL esterase/lipase At5g22810-like [Cucurbita moschata])

HSP 1 Score: 602 bits (1551), Expect = 2.44e-215
Identity = 303/350 (86.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRD 60
           MD TCL IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKTL+KANF PYGRD
Sbjct: 1   MDVTCLPIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDLLIGASFASAASGYLDTTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQAYLG  GGK+LLIGASFASAASGYLDTTA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGASFASAASGYLDTTA 120

Query: 121 ELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLY 180
            LYNALS TQQLE+YKEYQNKVAE AGK+NAS+IISGAIYLVSAGSNDFLQNYY+NPL+Y
Sbjct: 121 HLYNALSLTQQLEYYKEYQNKVAEFAGKTNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 KKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKL 240
           KKYTV+QF++I+I SY+ FIQNLYALGARRIGVTTLPPLGCLP AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFADILIASYVNFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGL 300
           NNDAV+FNSKLNATSQSL   L GLNLVVLD+YKPLY L+TKPAE+GF EARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYQLVTKPAENGFFEARKACCGTGL 300

Query: 301 LETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           LETS LCN ESVGTCANAS+YVFWDGFHPSEAANKFLA +LL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of CsGy6G012180 vs. NCBI nr
Match: XP_022995313.1 (GDSL esterase/lipase At5g22810-like [Cucurbita maxima])

HSP 1 Score: 601 bits (1549), Expect = 4.92e-215
Identity = 302/350 (86.29%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRD 60
           MD TCL+IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKTL+KANF PYGRD
Sbjct: 1   MDRTCLAIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDLLIGASFASAASGYLDTTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQAYLG  GGK+LLIG SFASAASGY+DTTA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGTSFASAASGYIDTTA 120

Query: 121 ELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLY 180
            LYNALS T+QLE+YKEYQNKVAE AGK NAS+IISGAIYLVSAGSNDFLQNYY+NPL+Y
Sbjct: 121 HLYNALSLTEQLEYYKEYQNKVAEFAGKPNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 KKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKL 240
           KKYTV+QF++I+ITSYI FIQNLYALGARRIGVTTLPPLGCLP AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFTDILITSYINFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGL 300
           NNDAV+FNSKLNATSQSL   L GLNLVVLD+YKPLY+L+TKPAE+GF EARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYELVTKPAENGFLEARKACCGTGL 300

Query: 301 LETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           LETS LCN ESVGTCANAS+YVFWDGFHPSEAANKFLA +LL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of CsGy6G012180 vs. ExPASy TrEMBL
Match: A0A0A0KAZ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149950 PE=3 SV=1)

HSP 1 Score: 684 bits (1766), Expect = 1.89e-248
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
           ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL
Sbjct: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN
Sbjct: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE
Sbjct: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS
Sbjct: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of CsGy6G012180 vs. ExPASy TrEMBL
Match: A0A5A7SW04 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00080 PE=3 SV=1)

HSP 1 Score: 667 bits (1720), Expect = 1.94e-241
Identity = 338/348 (97.13%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDTTCLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKD L+GASFASAASGYLDTTAEL
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDFLVGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALSFTQQLE+YKEYQNKVAEVAGK NASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YTVSQFS+IIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECV KLNN
Sbjct: 181 YTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAVAFNSKLNATSQSLRTKL GLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE
Sbjct: 241 DAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TSFLCNTESVGTCANAS+YVFWDGFHPSEAANKFLASSLLASGISLIS
Sbjct: 301 TSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of CsGy6G012180 vs. ExPASy TrEMBL
Match: A0A1S3C7W6 (GDSL esterase/lipase At5g22810 OS=Cucumis melo OX=3656 GN=LOC103497987 PE=3 SV=1)

HSP 1 Score: 667 bits (1720), Expect = 1.94e-241
Identity = 338/348 (97.13%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60
           MDTTCLSIFFLLLAPVI LANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF
Sbjct: 1   MDTTCLSIFFLLLAPVIPLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDF 60

Query: 61  ITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDLLIGASFASAASGYLDTTAEL 120
            THKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKD L+GASFASAASGYLDTTAEL
Sbjct: 61  TTHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLGGGGKDFLVGASFASAASGYLDTTAEL 120

Query: 121 YNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180
           YNALSFTQQLE+YKEYQNKVAEVAGK NASSIISGAIYLVSAGSNDFLQNYYINPLLYKK
Sbjct: 121 YNALSFTQQLEYYKEYQNKVAEVAGKPNASSIISGAIYLVSAGSNDFLQNYYINPLLYKK 180

Query: 181 YTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNN 240
           YTVSQFS+IIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECV KLNN
Sbjct: 181 YTVSQFSDIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVEKLNN 240

Query: 241 DAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300
           DAVAFNSKLNATSQSLRTKL GLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE
Sbjct: 241 DAVAFNSKLNATSQSLRTKLSGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLE 300

Query: 301 TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           TSFLCNTESVGTCANAS+YVFWDGFHPSEAANKFLASSLLASGISLIS
Sbjct: 301 TSFLCNTESVGTCANASEYVFWDGFHPSEAANKFLASSLLASGISLIS 348

BLAST of CsGy6G012180 vs. ExPASy TrEMBL
Match: A0A6J1H329 (GDSL esterase/lipase At5g22810-like OS=Cucurbita moschata OX=3662 GN=LOC111459655 PE=3 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 1.18e-215
Identity = 303/350 (86.57%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRD 60
           MD TCL IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKTL+KANF PYGRD
Sbjct: 1   MDVTCLPIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDLLIGASFASAASGYLDTTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQAYLG  GGK+LLIGASFASAASGYLDTTA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGASFASAASGYLDTTA 120

Query: 121 ELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLY 180
            LYNALS TQQLE+YKEYQNKVAE AGK+NAS+IISGAIYLVSAGSNDFLQNYY+NPL+Y
Sbjct: 121 HLYNALSLTQQLEYYKEYQNKVAEFAGKTNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 KKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKL 240
           KKYTV+QF++I+I SY+ FIQNLYALGARRIGVTTLPPLGCLP AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFADILIASYVNFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGL 300
           NNDAV+FNSKLNATSQSL   L GLNLVVLD+YKPLY L+TKPAE+GF EARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYQLVTKPAENGFFEARKACCGTGL 300

Query: 301 LETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           LETS LCN ESVGTCANAS+YVFWDGFHPSEAANKFLA +LL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of CsGy6G012180 vs. ExPASy TrEMBL
Match: A0A6J1K7K4 (GDSL esterase/lipase At5g22810-like OS=Cucurbita maxima OX=3661 GN=LOC111490896 PE=3 SV=1)

HSP 1 Score: 601 bits (1549), Expect = 2.38e-215
Identity = 302/350 (86.29%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVI-SLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRD 60
           MD TCL+IFFLLLAPV  SLANGQPLVPA+FTFGDSVLDVGINNHLKTL+KANF PYGRD
Sbjct: 1   MDRTCLAIFFLLLAPVSQSLANGQPLVPAMFTFGDSVLDVGINNHLKTLMKANFPPYGRD 60

Query: 61  FITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG-GGGKDLLIGASFASAASGYLDTTA 120
           F+TH PTGRFCNGKLASDFTAEYLGFTSYPQAYLG  GGK+LLIG SFASAASGY+DTTA
Sbjct: 61  FVTHNPTGRFCNGKLASDFTAEYLGFTSYPQAYLGEAGGKNLLIGTSFASAASGYIDTTA 120

Query: 121 ELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLY 180
            LYNALS T+QLE+YKEYQNKVAE AGK NAS+IISGAIYLVSAGSNDFLQNYY+NPL+Y
Sbjct: 121 HLYNALSLTEQLEYYKEYQNKVAEFAGKPNASAIISGAIYLVSAGSNDFLQNYYVNPLVY 180

Query: 181 KKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKL 240
           KKYTV+QF++I+ITSYI FIQNLYALGARRIGVTTLPPLGCLP AIT+FGSDSNECV KL
Sbjct: 181 KKYTVTQFTDILITSYINFIQNLYALGARRIGVTTLPPLGCLPTAITIFGSDSNECVGKL 240

Query: 241 NNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGL 300
           NNDAV+FNSKLNATSQSL   L GLNLVVLD+YKPLY+L+TKPAE+GF EARKACCGTGL
Sbjct: 241 NNDAVSFNSKLNATSQSLHANLPGLNLVVLDTYKPLYELVTKPAENGFLEARKACCGTGL 300

Query: 301 LETSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 348
           LETS LCN ESVGTCANAS+YVFWDGFHPSEAANKFLA +LL +GISLIS
Sbjct: 301 LETSILCNKESVGTCANASEYVFWDGFHPSEAANKFLADNLLPAGISLIS 350

BLAST of CsGy6G012180 vs. TAIR 10
Match: AT5G22810.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 1.7e-132
Identity = 233/331 (70.39%), Postives = 274/331 (82.78%), Query Frame = 0

Query: 23  QPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKPTGRFCNGKLASDFTAEY 82
           QPLVPA+F FGDSV+DVG NN + T++KANF PYGRDF TH PTGRFCNGKLA+DFTAE 
Sbjct: 7   QPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLATDFTAEN 66

Query: 83  LGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNALSFTQQLEHYKEYQNKV 142
           LGF SYPQAYL     GK+LLIGA+FASAASGY D TA+LY+A+S  QQLEHYK+Y +++
Sbjct: 67  LGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHYKDYISRI 126

Query: 143 AEVA---GKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTVSQFSEIIITSYIIF 202
            E+A     SNAS+IIS  IY+VSAGS+DF+QNYYINPLLY+  +  +FS+++I SY  F
Sbjct: 127 QEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLLILSYSSF 186

Query: 203 IQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNNDAVAFNSKLNATSQSLR 262
           IQNLY+LGARRIGVTTLPPLGCLPAAITV G     C  KLNNDA++FN+KLN TSQ L+
Sbjct: 187 IQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLNTTSQDLK 246

Query: 263 TKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLETSFLCNTESVGTCANAS 322
             L GLNLVV D Y+PLYDL T+P+E GF+EAR+ACCGTGLLETS LCN +SVGTC NA+
Sbjct: 247 RNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVGTCNNAT 306

Query: 323 QYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           +YVFWDGFHP+EAANK LA +LL SGISLIS
Sbjct: 307 EYVFWDGFHPTEAANKILADNLLVSGISLIS 337

BLAST of CsGy6G012180 vs. TAIR 10
Match: AT5G03810.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 421.8 bits (1083), Expect = 5.4e-118
Identity = 214/347 (61.67%), Postives = 261/347 (75.22%), Query Frame = 0

Query: 5   CLSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHK 64
           CLS+     A V     G+ LVPAL   GDSV+D G NNH  TL+KANF PYGRDF+ H 
Sbjct: 10  CLSVIACFYAGV---GTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHS 69

Query: 65  PTGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYN 124
            TGRF NGKLA+DFTAE LGFTSYP AYL       +LL GA+FAS ASG+ D TA  YN
Sbjct: 70  ATGRFSNGKLATDFTAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYN 129

Query: 125 ALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYT 184
           A++ +QQL++YKEYQNKV  + GK  A+ I SGAI+L+S GS+DFLQ+YYINP+L + +T
Sbjct: 130 AITLSQQLKNYKEYQNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFT 189

Query: 185 VSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGS-DSNECVAKLNND 244
             Q+S+ ++ SY  F+QNLY LGARRIGVTTLPPLGCLPAAIT+FG   +N CV +LN D
Sbjct: 190 PDQYSDHLLRSYSTFVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQD 249

Query: 245 AVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLET 304
           AV+FN+KLN TS +L   L GL LVV D Y PL +++  P E+GF E+R+ACCGTG +ET
Sbjct: 250 AVSFNTKLNNTSINLTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMET 309

Query: 305 SFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           SFLCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A++LL  GI LIS
Sbjct: 310 SFLCNALSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 353

BLAST of CsGy6G012180 vs. TAIR 10
Match: AT5G03820.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 419.1 bits (1076), Expect = 3.5e-117
Identity = 211/347 (60.81%), Postives = 258/347 (74.35%), Query Frame = 0

Query: 6   LSIFFLLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKP 65
           L  F ++      +  G+PLVPAL   GDSV+D G NN L TLIKANF PYGRDF+ H  
Sbjct: 8   LMTFSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNA 67

Query: 66  TGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNA 125
           TGRF NGKLA+DFTAE LGFTSYP  YL     G +LL GA+FAS ASGY D TA  YNA
Sbjct: 68  TGRFSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNA 127

Query: 126 LSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTV 185
           ++  QQL++YKEYQNKV  + G   A+ I SGAI+L+S GS+DFLQ+YYINP+L + +T 
Sbjct: 128 ITLNQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTP 187

Query: 186 SQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFG--SDSNECVAKLNND 245
            Q+S+ ++  Y  F+QNLY LGAR+IGVTTLPPLGCLPAAIT+FG   ++N CV +LN D
Sbjct: 188 DQYSDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQD 247

Query: 246 AVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLET 305
           AV+FN+KLN TS +L   L GL LVV D Y PL ++   P E+GF E+R+ACCGTG +ET
Sbjct: 248 AVSFNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVET 307

Query: 306 SFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           SFLCN  SVGTC+NA+ YVFWDGFHPSEAAN+ +A++LL  GI LIS
Sbjct: 308 SFLCNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLIS 354

BLAST of CsGy6G012180 vs. TAIR 10
Match: AT3G53100.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 397.9 bits (1021), Expect = 8.4e-111
Identity = 193/340 (56.76%), Postives = 255/340 (75.00%), Query Frame = 0

Query: 11  LLLAPVISLANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGRDFITHKPTGRFC 70
           LLL       +   +VPAL  FGDS++DVG NN+L +++K+NFLPYGRDFI  +PTGRFC
Sbjct: 12  LLLVSCFFCKSKGAVVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFC 71

Query: 71  NGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDTTAELYNALSFTQ 130
           NGKLA DF+AEYLGF+SYP A+L      +++LIGA+FASA+SGY D T+  + ++S T+
Sbjct: 72  NGKLAVDFSAEYLGFSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTR 131

Query: 131 QLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPLLYKKYTVSQFSE 190
           QL +Y+ YQN+V  + G+ NA  + S  I+++SAGS+DFLQNYYINPLL    T  QF++
Sbjct: 132 QLSYYRAYQNRVTRMIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFAD 191

Query: 191 IIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVAKLNNDAVAFNSK 250
           I++ S+  FIQNLY LGARRIGV +LPP+GCLPAAIT+FG+ +  CV +LNNDA+ FN+K
Sbjct: 192 ILLRSFSEFIQNLYELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTK 251

Query: 251 LNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGTGLLETSFLCNTE 310
           L  T++ L  +  GL LV  + Y+P  D+IT P ++GF E ++ACCGTG +ETSFLCN+ 
Sbjct: 252 LENTTRLLMNRHSGLRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSL 311

Query: 311 SVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLIS 349
           S GTC NA+ YVFWDGFHP+EA N+ LA  LL  GISLI+
Sbjct: 312 SFGTCVNATGYVFWDGFHPTEAVNELLAGQLLGQGISLIN 351

BLAST of CsGy6G012180 vs. TAIR 10
Match: AT3G16370.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 9.3e-110
Identity = 200/352 (56.82%), Postives = 255/352 (72.44%), Query Frame = 0

Query: 1   MDTTCLSIFFLLLAPVISL--ANGQPLVPALFTFGDSVLDVGINNHLKTLIKANFLPYGR 60
           MD    S   L L   +S+   +   LVPA+ TFGDSV+DVG NN+L TL +A++ PYGR
Sbjct: 1   MDRCTSSFLLLTLVSTLSILQISFAQLVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGR 60

Query: 61  DFITHKPTGRFCNGKLASDFTAEYLGFTSYPQAYLG--GGGKDLLIGASFASAASGYLDT 120
           DF  HK TGRFCNGKLA+D TAE LGFT YP AYL     GK+LLIGA+FASAASGY D 
Sbjct: 61  DFANHKATGRFCNGKLATDITAETLGFTKYPPAYLSPEASGKNLLIGANFASAASGYDDK 120

Query: 121 TAELYNALSFTQQLEHYKEYQNKVAEVAGKSNASSIISGAIYLVSAGSNDFLQNYYINPL 180
            A L +A+   QQ+E++KEY++K+ ++AG   A SII GAI L+SAGS+DF+QNYY+NPL
Sbjct: 121 AALLNHAIPLYQQVEYFKEYKSKLIKIAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPL 180

Query: 181 LYKKYTVSQFSEIIITSYIIFIQNLYALGARRIGVTTLPPLGCLPAAITVFGSDSNECVA 240
           LYK YTV  +   +I ++  FI+ +YA+GAR+IGVT+LPP GCLPAA T+FG     CV+
Sbjct: 181 LYKVYTVDAYGSFLIDNFSTFIKQVYAVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVS 240

Query: 241 KLNNDAVAFNSKLNATSQSLRTKLYGLNLVVLDSYKPLYDLITKPAEHGFSEARKACCGT 300
           +LN DA  FN KLNA +  L+ +   L +VV D Y PLYDL+  P++ GF+EA K CCGT
Sbjct: 241 RLNTDAQNFNKKLNAAASKLQKQYSDLKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGT 300

Query: 301 GLLE-TSFLCNTESVGTCANASQYVFWDGFHPSEAANKFLASSLLASGISLI 348
           G +E TS LCN +S GTC+NA+QYVFWD  HPSEAAN+ LA++L+  G SL+
Sbjct: 301 GTVETTSLLCNPKSFGTCSNATQYVFWDSVHPSEAANEILATALIGQGFSLL 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFC61.1e-13169.23GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3... [more]
Q9FFN07.7e-11761.67GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3... [more]
Q9LZC55.0e-11660.81GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3... [more]
Q0WPI91.2e-10956.76GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2... [more]
Q9LU141.3e-10856.82GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004145774.13.90e-248100.00GDSL esterase/lipase At5g22810 [Cucumis sativus] >KGN46905.1 hypothetical protei... [more]
XP_008458651.14.01e-24197.13PREDICTED: GDSL esterase/lipase At5g22810 [Cucumis melo] >KAA0033359.1 GDSL este... [more]
XP_038901478.12.26e-22692.53GDSL esterase/lipase At5g22810 [Benincasa hispida][more]
XP_022958433.12.44e-21586.57GDSL esterase/lipase At5g22810-like [Cucurbita moschata][more]
XP_022995313.14.92e-21586.29GDSL esterase/lipase At5g22810-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KAZ31.89e-248100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149950 PE=3 SV=1[more]
A0A5A7SW041.94e-24197.13GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G... [more]
A0A1S3C7W61.94e-24197.13GDSL esterase/lipase At5g22810 OS=Cucumis melo OX=3656 GN=LOC103497987 PE=3 SV=1[more]
A0A6J1H3291.18e-21586.57GDSL esterase/lipase At5g22810-like OS=Cucurbita moschata OX=3662 GN=LOC11145965... [more]
A0A6J1K7K42.38e-21586.29GDSL esterase/lipase At5g22810-like OS=Cucurbita maxima OX=3661 GN=LOC111490896 ... [more]
Match NameE-valueIdentityDescription
AT5G22810.11.7e-13270.39GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03810.15.4e-11861.67GDSL-like Lipase/Acylhydrolase family protein [more]
AT5G03820.13.5e-11760.81GDSL-like Lipase/Acylhydrolase family protein [more]
AT3G53100.18.4e-11156.76GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G16370.19.3e-11056.82GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 346..348
NoneNo IPR availablePANTHERPTHR45642:SF103GDSL-MOTIF LIPASEcoord: 8..347
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 8..347
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 147..339
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 29..338
e-value: 2.1E-39
score: 136.3
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 19..343
e-value: 2.6E-72
score: 245.9
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 27..340
e-value: 6.82111E-132
score: 377.341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G012180.2CsGy6G012180.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds