CsGy2G023300 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G023300
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionL-ascorbate peroxidase
LocationGy14Chr2: 31328812 .. 31332297 (-)
RNA-Seq ExpressionCsGy2G023300
SyntenyCsGy2G023300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTCCACCTTCTTCAATCCTGCGCCGTCGTGCAACGTCGCCGTCATTTCTTCTTCCTTTCTCAGATTCAAGTTCATACCGAAATTTCCCACCACCATACCTTCATCGTCTGTTTCGACGGTCTCCTTTCGCGCCAAAACTACCGTTAACTGTTCTCTCCCCGCCCCTGGCCATGCTTCTGGTAAAATATTAATTAACTGCTTTCCTCTTCCTTTGTGCAAAGCCTTGGTCAATTCCGATGGGTTTTGCCTATTCAATTTTCGGAACTTTGTATTTGACTGCTATTGTCTATCCTGTGTATCTGTATTTGGTTTGTGGCTTCTGAAACCAAAATCTGTATTATGCATTTTGCAATGGCGAACTGATCCTTTAAAGTACTGTTTGAATTACAGTTCAGACTGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCCTTTCTTCTTATTTTGCATGAATCCGTGGAAGGTTTTAAAGCTGAGGCGGCAGAGTAAGATGGAGGATGAGGATATTGCTTACGATTAGTTTGAAGTATTTAATATAAAACTTTCTGTATTCTAGAATCTTGTTCATTATTGAAAACTCATGAATTCTTATGTCCTAGTAGAGGGATTATTAACTGAGTTCTGTCTGTTTTCTTTTAGGGGCAGAAGATTGGATAAAACGGAAGTTGAACGGATAAGAGAAGAAGTAAGAAAGGTGGTAACCAAAGGAAGGGCTCCTGGTCTGCTTCGTTTGGTATTTCACGATGCTGGAACTTTTGAAACCAATGATACTTCAGGTTAATATCCTTAAACTTTAAGAGGAGAGGCAATGAGGTTTTCTTAGGCGTCCTGAATTATTTGAATTCATACATAGATTTTAAATTTTGAGTTCAATTGTTGGAATTCAGACAGCATTGGATATGTTGTATGAACTGTTATTTGCACCAAATGTGAAGTTTTGTTCGATCACGGAGGCCATGATATCCACTTCCATGTAGGCTTTCAGACATGTTTGTTCTTGTATAGTTTTCAGTATGATTGTCATTTTAGTGAGCCTTGTTTGCAGGTGGTATGAACGGATCTATAGTTCATGAACTTGATAGACCTGAAAACAAAGGTCTCAAGAAATCAGTTAAGGTGGTTCTTTCTTCTTATTCCCTTTTTGTTTTAGGAATCTGTCTACCAGCAAGCTCATCATTGGTTTTGTTGTTTCTTAGATTCTTCAGGAAGCAAAGAGTACACTGGACTTAATTAGACCTGGTAAGTTTACTCTCTATTTCTCCATTTCTATCTGAAAGGAGATGAAGTTTCCATAATAAGAGGTATTGACTAGTAGCCAGGCTAACTTCTTAGCAAATTGGTTTCTTTTGAATAGTATCGTGGGCAGACGTGATAGTAGTGGCTGGTGCTGAAGCAGTTTCAATATGTGGGGGTCCAAGTATTGCTGTTGATTTGGGTAGATTAGACTCGGAGTAAGCTGAAGCTGGCTGTTTTCCTTGTTATATGATGAACCATATTTTCCTTGAGTTGCAGCCTGTTAACGTTCTTTTTGCTTGTTTTTCCATGAGCTTTATGTACCAGAATCATTTGTTTCTCTGGACTATGATAGTTACATTCTCACCATTTTAACTTTTCCCATGGGCTTTGTGCACAAGAGAAAGTTCCGAATTCTGAGATATCCTCTCTCTCACATTCATCACAATAGATAGATCCCTCTTCACTGTGTACACTCTCTTTTTCTCCCATACATCATTTCTACCTATGCTCTGCTGACTAGCTCTCTAACCGAGTTTTAGTCCTCATGATAGTAGACCATTTTAGCTAATGAATTTCCTGTGTTCCCACTGCCGAACTCTTTATCTTTCTCATCAATTCCTCTGTCCGTCATATGATAGAAGGTTTGTCATATTAATTTTAGCTGTTTTTCTTTTTCCAATTGTTTCTTTCCTTTCCAAATAGGAAGCCGGATCCAGAAGGTAAACTTCCTGAGGAATCTCTGGATGCTGTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTTCGTGAGTGCATCTATCTCATTTTGACTCTTCCATTTGAAGGAGCTGTAGTCACTGGACTGTTTATATATTTGTTATTTATTTTAATTTCTTTTTTTCTTGGCCAATATATTCTAAGTTACTGCATTGCTCTCCTTCATATATTTATTTACTAATGTGGCCGAATCTCTAAGATGTAATCTGGATTCGTTTCCTTACAGTAGACATAAAATGGCTAAAAAGTCTATTATTAGAACTTTAGGAATGGGAAATCCTAGTTCTGCATTTGATTGTACAACATAGTCCTTTCAAATGTGTGTTGAACAGTGTTGTTTTTTATGAAAACCAACTCAACCCTCCCACTGTGAAGTATATTCGCATGGACAGTAGTAGAGAAGTTAAAATGCTCGTCACTCTAGAAATATGCCCATCCTCTGTCCTATCCTTTCTTCTTTCACATATTTTTTTACAAAGAAACAATTTCATTGGTATATGAAATTGGAGAGTAGAACTCCAAATGAATGCCAGATAATTCATAATAGAATGAATTTACACCTCCAAGGTGTCTTCTGCATGATGACATTCACGGATACTCTTTTTTCTTTGCAGGACACGAGAACTAGTTGCACTATCTGGAGCCCATACTATTGGTGGGAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATGCATATTTCAAAATACTTTTGGAGAAGCCATGGTCATCTAACGGTAATCAATCTCAGAATGATACTTTCACACGTCTCATTTTGTTTGAATTGAGCCTCAATTGCAAATCATTGGCTGCCTGAGGCACTCCCTCAATTTTTGCTCAAATCCACATTATTAGTGTTCCCGTGAATCAGTGGGTAACGAAAAATGAAAGCTAGTTTTTTGTTTTTCTAGTTGAAGTGAGCTATGAAGTTCTTGTGATTACTCAAACTGAGTTGTTACTTTTGTGGCTTTTGCCATTGATTAATAATACAAGTCATTGCTTTGAATTGGTTTATACATATTTCCACTCGAGCCTTATTTTATTTTTTAATTTTAGGTGGTATGTCAAGTATGATAGGGCTACCTTCTGATCGAGCTCTTGCTGACGATGATGAATGCTTGAGGTTCTTCTCTTTCATTCAAATCTATACTATTGTCATCATTACTTTTCTGCACCACTGTGAACTGATGCCATTTCTGGTCTCTTGAGTAGAATTTCAGCAATGAGTTCAATTTGTTGAACTAAAGTAGAAATGTTCATAAAAGATCAATTTTACAACCAGACAACCAGCAAGATTTACCTGATAAACAGAAAATGAGCTGATTACCCCTCTTTATCTGCTTAAATAAAGTGAATTACTTTGAACTCCTAGATGGATCAAAGAGTATGCCAAGGATCAGAATGTGTTCTTCGAAGATTTCCATAATGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAGAAGCTAG

mRNA sequence

ATGACTTCCACCTTCTTCAATCCTGCGCCGTCGTGCAACGTCGCCGTCATTTCTTCTTCCTTTCTCAGATTCAAGTTCATACCGAAATTTCCCACCACCATACCTTCATCGTCTGTTTCGACGGTCTCCTTTCGCGCCAAAACTACCGTTAACTGTTCTCTCCCCGCCCCTGGCCATGCTTCTGTTCAGACTGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCCTTTCTTCTTATTTTGCATGAATCCGTGGAAGGTTTTAAAGCTGAGGCGGCAGAGGGCAGAAGATTGGATAAAACGGAAGTTGAACGGATAAGAGAAGAAGTAAGAAAGGTGGTAACCAAAGGAAGGGCTCCTGGTCTGCTTCGTTTGGTATTTCACGATGCTGGAACTTTTGAAACCAATGATACTTCAGGTGGTATGAACGGATCTATAGTTCATGAACTTGATAGACCTGAAAACAAAGGTCTCAAGAAATCAGTTAAGATTCTTCAGGAAGCAAAGAGTACACTGGACTTAATTAGACCTGTATCGTGGGCAGACGTGATAGTAGTGGCTGGTGCTGAAGCAGTTTCAATATGTGGGGGTCCAAGTATTGCTGTTGATTTGGGTAGATTAGACTCGGAGAAGCCGGATCCAGAAGGTAAACTTCCTGAGGAATCTCTGGATGCTGTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTTCGACACGAGAACTAGTTGCACTATCTGGAGCCCATACTATTGGTGGGAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATGCATATTTCAAAATACTTTTGGAGAAGCCATGGTCATCTAACGGTGGTATGTCAAGTATGATAGGGCTACCTTCTGATCGAGCTCTTGCTGACGATGATGAATGCTTGAGATGGATCAAAGAGTATGCCAAGGATCAGAATGTGTTCTTCGAAGATTTCCATAATGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAGAAGCTAG

Coding sequence (CDS)

ATGACTTCCACCTTCTTCAATCCTGCGCCGTCGTGCAACGTCGCCGTCATTTCTTCTTCCTTTCTCAGATTCAAGTTCATACCGAAATTTCCCACCACCATACCTTCATCGTCTGTTTCGACGGTCTCCTTTCGCGCCAAAACTACCGTTAACTGTTCTCTCCCCGCCCCTGGCCATGCTTCTGTTCAGACTGATTTCTGCTTTCTGAAGAAGAGGGAATTTGTCTTTTCTGCCGCGCTGCCCTTTCTTCTTATTTTGCATGAATCCGTGGAAGGTTTTAAAGCTGAGGCGGCAGAGGGCAGAAGATTGGATAAAACGGAAGTTGAACGGATAAGAGAAGAAGTAAGAAAGGTGGTAACCAAAGGAAGGGCTCCTGGTCTGCTTCGTTTGGTATTTCACGATGCTGGAACTTTTGAAACCAATGATACTTCAGGTGGTATGAACGGATCTATAGTTCATGAACTTGATAGACCTGAAAACAAAGGTCTCAAGAAATCAGTTAAGATTCTTCAGGAAGCAAAGAGTACACTGGACTTAATTAGACCTGTATCGTGGGCAGACGTGATAGTAGTGGCTGGTGCTGAAGCAGTTTCAATATGTGGGGGTCCAAGTATTGCTGTTGATTTGGGTAGATTAGACTCGGAGAAGCCGGATCCAGAAGGTAAACTTCCTGAGGAATCTCTGGATGCTGTTGGATTGAAGCAAAGCTTCAGCAGAAAAGGATTTTCGACACGAGAACTAGTTGCACTATCTGGAGCCCATACTATTGGTGGGAAAGGTTTTGGAAGCCCTGTTGTTTTTGATAATGCATATTTCAAAATACTTTTGGAGAAGCCATGGTCATCTAACGGTGGTATGTCAAGTATGATAGGGCTACCTTCTGATCGAGCTCTTGCTGACGATGATGAATGCTTGAGATGGATCAAAGAGTATGCCAAGGATCAGAATGTGTTCTTCGAAGATTTCCATAATGCTTATATCAAGCTAGTAAATTCTGGTGCAAAGTGGAGAAGCTAG

Protein sequence

MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHASVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVTKGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRKGFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS*
Homology
BLAST of CsGy2G023300 vs. ExPASy Swiss-Prot
Match: Q8GY91 (Putative L-ascorbate peroxidase 6 OS=Arabidopsis thaliana OX=3702 GN=APX6 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.0e-94
Identity = 167/226 (73.89%), Postives = 195/226 (86.28%), Query Frame = 0

Query: 111 IREEVRKVVTKGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKIL 170
           ++ E+RKVVTKG+A G+LRLVFHDAGTFE +D SGG+NGSI +EL+RPEN GLKKS+K+L
Sbjct: 101 MQNEIRKVVTKGKAAGVLRLVFHDAGTFELDDHSGGINGSIAYELERPENIGLKKSLKVL 160

Query: 171 QEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDA 230
            +AK  +D I+PVSWAD+I VAG+EAVSICGGP+I V LGRLDS +PDPEGKLP E+L A
Sbjct: 161 AKAKVKVDEIQPVSWADMISVAGSEAVSICGGPTIPVVLGRLDSAQPDPEGKLPPETLSA 220

Query: 231 VGLKQSFSRKGFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMI 290
            GLK+ F RKGFST+ELVALSGAHTIG KGFG P VFDNAY+KILLEKPW+S   M+SM+
Sbjct: 221 SGLKECFKRKGFSTQELVALSGAHTIGSKGFGDPTVFDNAYYKILLEKPWTSTSKMTSMV 280

Query: 291 GLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKW 337
           GLPSD AL  DDECLRW+K YA+DQ+ FFEDF NAYIKLVNSGAKW
Sbjct: 281 GLPSDHALVQDDECLRWVKRYAEDQDKFFEDFTNAYIKLVNSGAKW 326

BLAST of CsGy2G023300 vs. ExPASy Swiss-Prot
Match: Q1PER6 (L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX2 PE=2 SV=3)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 92/242 (38.02%), Postives = 139/242 (57.44%), Query Frame = 0

Query: 105 KTEVERIREEVRKVVT-KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVH--ELDRPENK 164
           K  V+R + ++R ++  K  AP +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 165 GLKKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEG 224
           GL  +V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 225 KLPEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAY 284
           +LP+ +     L+  F R G + +++VALSG HT+G          G    +P++FDN+Y
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 285 FKILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVN 334
           FK +L      +G    ++ LP+D+AL DD   L ++++YA D++ FFED+  A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of CsGy2G023300 vs. ExPASy Swiss-Prot
Match: Q6ZJJ1 (Probable L-ascorbate peroxidase 4, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=APX4 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.5e-34
Identity = 89/245 (36.33%), Postives = 140/245 (57.14%), Query Frame = 0

Query: 107 EVERIREEVRKVV-TKGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELD--RPENKGL 166
           +V+R R  +R ++ +KG AP +LRL +HDAGT++ N  +GG NGSI +E +     N GL
Sbjct: 13  QVDRARRHLRALISSKGCAPIMLRLAWHDAGTYDVNTKTGGANGSIRYEEEYTHGSNAGL 72

Query: 167 KKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKL 226
           K ++ +L+  K+       +++AD+  +AG  AV + GGP++    GR DS     EG+L
Sbjct: 73  KIAIDLLEPIKAKSP---KITYADLYQLAGVVAVEVTGGPTVEFIPGRRDSSVCPREGRL 132

Query: 227 PEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAYFK 286
           P+    A+ L+  F R G S +++VALSG HT+G          G     P+ FDN+YF 
Sbjct: 133 PDAKKGALHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFEGAWTQEPLKFDNSYFL 192

Query: 287 ILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSG 339
            LL+      G    ++ LP+D+AL +D    R++  YA+D++ FF+D+  ++ KL   G
Sbjct: 193 ELLK------GESEGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHKKLSELG 248

BLAST of CsGy2G023300 vs. ExPASy Swiss-Prot
Match: Q7XZP5 (L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=APX5 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 4.6e-34
Identity = 92/240 (38.33%), Postives = 141/240 (58.75%), Query Frame = 0

Query: 107 EVERIREEVRKVV-TKGRAPGLLRLVFHDAGTFETNDTSGGMNGSI--VHELDRPENKGL 166
           E+E+ R ++R ++ ++  AP +LRL +HDAGT++    +GG NGSI    EL+RP NKGL
Sbjct: 12  EIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELNRPHNKGL 71

Query: 167 KKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKL 226
           +K+V   +E K+       VS+AD+  +AG  AV + GGP+I    GR D++  D +G+L
Sbjct: 72  EKAVAFCEEVKAKHP---RVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSAD-DGEL 131

Query: 227 PEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAYFK 286
           P  +  A  L+  FSR G   R++VALSG HT+G          G     P+ FDN+YF 
Sbjct: 132 PNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDNSYFV 191

Query: 287 ILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSG 334
            LL+      G    ++ L +D+AL DD +   ++K YAKD+++FF+ +  ++ KL   G
Sbjct: 192 ELLK------GETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELG 241

BLAST of CsGy2G023300 vs. ExPASy Swiss-Prot
Match: Q01MI9 (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica OX=39946 GN=APX3 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.7e-33
Identity = 89/240 (37.08%), Postives = 137/240 (57.08%), Query Frame = 0

Query: 107 EVERIREEVRKVV-TKGRAPGLLRLVFHDAGTFETNDTSGGMNGSI--VHELDRPENKGL 166
           EVER R ++R ++ +K  AP +LRL +HDAGT++    +GG NGSI    E     N G+
Sbjct: 14  EVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFPQEYSHAANAGI 73

Query: 167 KKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKL 226
           K ++ +L+  K        +++AD+  +AG  AV + GGP+I    GR DS     EG+L
Sbjct: 74  KIAIDLLEPMKQRHP---KITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSSDSPEEGRL 133

Query: 227 PEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAYFK 286
           P+    A  L++ F R G S +++VALSG HT+G          G     P+ FDN+YF 
Sbjct: 134 PDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPLKFDNSYFI 193

Query: 287 ILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSG 334
            LL++  +S G    ++ LP+D+AL +D    R+++ YAKD++ FF D+  ++ KL   G
Sbjct: 194 ELLKE--NSEG----LLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSELG 244

BLAST of CsGy2G023300 vs. NCBI nr
Match: XP_004138724.1 (putative L-ascorbate peroxidase 6 isoform X1 [Cucumis sativus] >XP_011649824.1 putative L-ascorbate peroxidase 6 isoform X1 [Cucumis sativus] >KGN62952.1 hypothetical protein Csa_022225 [Cucumis sativus])

HSP 1 Score: 672 bits (1733), Expect = 1.92e-243
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA
Sbjct: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338

BLAST of CsGy2G023300 vs. NCBI nr
Match: XP_008445223.1 (PREDICTED: putative L-ascorbate peroxidase 6 isoform X4 [Cucumis melo] >KAA0064923.1 putative L-ascorbate peroxidase 6 isoform X4 [Cucumis melo var. makuwa])

HSP 1 Score: 625 bits (1613), Expect = 3.48e-225
Identity = 320/338 (94.67%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYAKDQNVFFEDF NAYIKLVNSGAKWRS
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFQNAYIKLVNSGAKWRS 336

BLAST of CsGy2G023300 vs. NCBI nr
Match: XP_038885450.1 (putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida] >XP_038885451.1 putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida])

HSP 1 Score: 612 bits (1577), Expect = 1.06e-219
Identity = 309/338 (91.42%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           MTSTFFNPAP CN AV+SSSFLRF FIPKFP  IP SS+STV FRAKTTVNCSLPA G A
Sbjct: 1   MTSTFFNPAPPCNAAVVSSSFLRFGFIPKFPAIIPLSSLSTVPFRAKTTVNCSLPAHGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFCFLKKREFVFSAALPFLLIL ESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILAESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFE NDTSGGMNGSI+HELDRPEN+GLKKSVKIL+EAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFEMNDTSGGMNGSIIHELDRPENRGLKKSVKILEEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVI VAGAEAVS+CGGPSI+V+LGRLDSEKPDPEGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIAVAGAEAVSVCGGPSISVELGRLDSEKPDPEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GF+TRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSD ALAD
Sbjct: 241 GFATRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDHALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYA DQNVFFEDF NAYIKLVNSGAKW+S
Sbjct: 301 DDECLRWIKEYANDQNVFFEDFQNAYIKLVNSGAKWKS 336

BLAST of CsGy2G023300 vs. NCBI nr
Match: XP_038885452.1 (putative L-ascorbate peroxidase 6 isoform X2 [Benincasa hispida])

HSP 1 Score: 609 bits (1571), Expect = 8.42e-219
Identity = 308/338 (91.12%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           MTSTFFNPAP CN AV+SSSFLRF FIPKFP  IP SS+STV FRAKTTVNCSLPA G A
Sbjct: 1   MTSTFFNPAPPCNAAVVSSSFLRFGFIPKFPAIIPLSSLSTVPFRAKTTVNCSLPAHGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFCFLKKREFVFSAALPFLLIL ESVEGFKAEAAE   LDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILAESVEGFKAEAAE---LDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFE NDTSGGMNGSI+HELDRPEN+GLKKSVKIL+EAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFEMNDTSGGMNGSIIHELDRPENRGLKKSVKILEEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVI VAGAEAVS+CGGPSI+V+LGRLDSEKPDPEGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIAVAGAEAVSVCGGPSISVELGRLDSEKPDPEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GF+TRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSD ALAD
Sbjct: 241 GFATRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDHALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYA DQNVFFEDF NAYIKLVNSGAKW+S
Sbjct: 301 DDECLRWIKEYANDQNVFFEDFQNAYIKLVNSGAKWKS 335

BLAST of CsGy2G023300 vs. NCBI nr
Match: XP_008445219.1 (PREDICTED: putative L-ascorbate peroxidase 6 isoform X1 [Cucumis melo])

HSP 1 Score: 558 bits (1439), Expect = 1.56e-198
Identity = 289/307 (94.14%), Postives = 294/307 (95.77%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRW 307
           DDECLR+
Sbjct: 301 DDECLRF 305

BLAST of CsGy2G023300 vs. ExPASy TrEMBL
Match: A0A0A0LQ87 (L-ascorbate peroxidase OS=Cucumis sativus OX=3659 GN=Csa_2G381780 PE=3 SV=1)

HSP 1 Score: 672 bits (1733), Expect = 9.29e-244
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA
Sbjct: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT
Sbjct: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338

BLAST of CsGy2G023300 vs. ExPASy TrEMBL
Match: A0A5A7V9T2 (L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002410 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.68e-225
Identity = 320/338 (94.67%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYAKDQNVFFEDF NAYIKLVNSGAKWRS
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFQNAYIKLVNSGAKWRS 336

BLAST of CsGy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3BBP4 (L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.68e-225
Identity = 320/338 (94.67%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKWRS 338
           DDECLRWIKEYAKDQNVFFEDF NAYIKLVNSGAKWRS
Sbjct: 301 DDECLRWIKEYAKDQNVFFEDFQNAYIKLVNSGAKWRS 336

BLAST of CsGy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3BC45 (L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 7.57e-199
Identity = 289/307 (94.14%), Postives = 294/307 (95.77%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE  RLDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE--RLDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRW 307
           DDECLR+
Sbjct: 301 DDECLRF 305

BLAST of CsGy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3BCW6 (L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 5.98e-198
Identity = 288/307 (93.81%), Postives = 293/307 (95.44%), Query Frame = 0

Query: 1   MTSTFFNPAPSCNVAVISSSFLRFKFIPKFPTTIPSSSVSTVSFRAKTTVNCSLPAPGHA 60
           M STFFNPAP CNVAV+SSSFLRFKFIPKFP T+P SS+STVSFRAKTTVN SL APG A
Sbjct: 1   MISTFFNPAPPCNVAVVSSSFLRFKFIPKFPATVPLSSLSTVSFRAKTTVNRSLCAPGDA 60

Query: 61  SVQTDFCFLKKREFVFSAALPFLLILHESVEGFKAEAAEGRRLDKTEVERIREEVRKVVT 120
           SVQTDFC LKKREFVFSAALPFLLILHESVEGFKAEAAE   LDK EVERIREEVRKVVT
Sbjct: 61  SVQTDFCLLKKREFVFSAALPFLLILHESVEGFKAEAAE---LDKREVERIREEVRKVVT 120

Query: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKILQEAKSTLDLI 180
           KGRAPGLLRLVFHDAGTFETNDTSGGMNGSI+HELDRPENKGLKKSVKILQEAKSTLDLI
Sbjct: 121 KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIIHELDRPENKGLKKSVKILQEAKSTLDLI 180

Query: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDAVGLKQSFSRK 240
           RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPD EGKLPEESLDAVGLKQ FSRK
Sbjct: 181 RPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDLEGKLPEESLDAVGLKQIFSRK 240

Query: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300
           GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD
Sbjct: 241 GFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMIGLPSDRALAD 300

Query: 301 DDECLRW 307
           DDECLR+
Sbjct: 301 DDECLRF 304

BLAST of CsGy2G023300 vs. TAIR 10
Match: AT4G32320.1 (ascorbate peroxidase 6 )

HSP 1 Score: 348.2 bits (892), Expect = 7.4e-96
Identity = 167/226 (73.89%), Postives = 195/226 (86.28%), Query Frame = 0

Query: 111 IREEVRKVVTKGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPENKGLKKSVKIL 170
           ++ E+RKVVTKG+A G+LRLVFHDAGTFE +D SGG+NGSI +EL+RPEN GLKKS+K+L
Sbjct: 101 MQNEIRKVVTKGKAAGVLRLVFHDAGTFELDDHSGGINGSIAYELERPENIGLKKSLKVL 160

Query: 171 QEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKLPEESLDA 230
            +AK  +D I+PVSWAD+I VAG+EAVSICGGP+I V LGRLDS +PDPEGKLP E+L A
Sbjct: 161 AKAKVKVDEIQPVSWADMISVAGSEAVSICGGPTIPVVLGRLDSAQPDPEGKLPPETLSA 220

Query: 231 VGLKQSFSRKGFSTRELVALSGAHTIGGKGFGSPVVFDNAYFKILLEKPWSSNGGMSSMI 290
            GLK+ F RKGFST+ELVALSGAHTIG KGFG P VFDNAY+KILLEKPW+S   M+SM+
Sbjct: 221 SGLKECFKRKGFSTQELVALSGAHTIGSKGFGDPTVFDNAYYKILLEKPWTSTSKMTSMV 280

Query: 291 GLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSGAKW 337
           GLPSD AL  DDECLRW+K YA+DQ+ FFEDF NAYIKLVNSGAKW
Sbjct: 281 GLPSDHALVQDDECLRWVKRYAEDQDKFFEDFTNAYIKLVNSGAKW 326

BLAST of CsGy2G023300 vs. TAIR 10
Match: AT3G09640.1 (ascorbate peroxidase 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-36
Identity = 92/242 (38.02%), Postives = 139/242 (57.44%), Query Frame = 0

Query: 105 KTEVERIREEVRKVVT-KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVH--ELDRPENK 164
           K  V+R + ++R ++  K  AP +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 165 GLKKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEG 224
           GL  +V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 225 KLPEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAY 284
           +LP+ +     L+  F R G + +++VALSG HT+G          G    +P++FDN+Y
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 285 FKILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVN 334
           FK +L      +G    ++ LP+D+AL DD   L ++++YA D++ FFED+  A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of CsGy2G023300 vs. TAIR 10
Match: AT3G09640.2 (ascorbate peroxidase 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-36
Identity = 92/242 (38.02%), Postives = 139/242 (57.44%), Query Frame = 0

Query: 105 KTEVERIREEVRKVVT-KGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVH--ELDRPENK 164
           K  V+R + ++R ++  K  AP +LRL +H AGTF+    +GG  G+I H  EL    N 
Sbjct: 14  KKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANN 73

Query: 165 GLKKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEG 224
           GL  +V++L   K   +L   +S+AD   +AG  AV I GGP I    GRLD  +P PEG
Sbjct: 74  GLDIAVRLLDPIK---ELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEG 133

Query: 225 KLPEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAY 284
           +LP+ +     L+  F R G + +++VALSG HT+G          G    +P++FDN+Y
Sbjct: 134 RLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSY 193

Query: 285 FKILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVN 334
           FK +L      +G    ++ LP+D+AL DD   L ++++YA D++ FFED+  A++KL  
Sbjct: 194 FKEIL------SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

BLAST of CsGy2G023300 vs. TAIR 10
Match: AT4G35970.1 (ascorbate peroxidase 5 )

HSP 1 Score: 146.7 bits (369), Expect = 3.3e-35
Identity = 92/240 (38.33%), Postives = 141/240 (58.75%), Query Frame = 0

Query: 107 EVERIREEVRKVV-TKGRAPGLLRLVFHDAGTFETNDTSGGMNGSI--VHELDRPENKGL 166
           E+E+ R ++R ++ ++  AP +LRL +HDAGT++    +GG NGSI    EL+RP NKGL
Sbjct: 12  EIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELNRPHNKGL 71

Query: 167 KKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKL 226
           +K+V   +E K+       VS+AD+  +AG  AV + GGP+I    GR D++  D +G+L
Sbjct: 72  EKAVAFCEEVKAKHP---RVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSAD-DGEL 131

Query: 227 PEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAYFK 286
           P  +  A  L+  FSR G   R++VALSG HT+G          G     P+ FDN+YF 
Sbjct: 132 PNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDNSYFV 191

Query: 287 ILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSG 334
            LL+      G    ++ L +D+AL DD +   ++K YAKD+++FF+ +  ++ KL   G
Sbjct: 192 ELLK------GETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELG 241

BLAST of CsGy2G023300 vs. TAIR 10
Match: AT4G35000.1 (ascorbate peroxidase 3 )

HSP 1 Score: 136.3 bits (342), Expect = 4.4e-32
Identity = 85/240 (35.42%), Postives = 130/240 (54.17%), Query Frame = 0

Query: 107 EVERIREEVRKVV-TKGRAPGLLRLVFHDAGTFETNDTSGGMNGSIVHELDRPE--NKGL 166
           E+ + R E+R ++  K  AP +LRL +HDAGT++    +GG NGSI +E +     N GL
Sbjct: 13  EITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNEEEHTHGANSGL 72

Query: 167 KKSVKILQEAKSTLDLIRPVSWADVIVVAGAEAVSICGGPSIAVDLGRLDSEKPDPEGKL 226
           K ++ + +  K+       +++AD+  +AG  AV + GGP I    GR DS     EG+L
Sbjct: 73  KIALDLCEGVKAKHP---KITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSNVCPKEGRL 132

Query: 227 PEESLDAVGLKQSFSRKGFSTRELVALSGAHTIG----------GKGFGSPVVFDNAYFK 286
           P+       L+  F R G S +++VALSG HT+G          G     P+ FDN+YF 
Sbjct: 133 PDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPLKFDNSYFV 192

Query: 287 ILLEKPWSSNGGMSSMIGLPSDRALADDDECLRWIKEYAKDQNVFFEDFHNAYIKLVNSG 334
            LL+      G    ++ LP+D+ L +D E  R ++ YAKD++ FF D+  ++ KL   G
Sbjct: 193 ELLK------GESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELG 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GY911.0e-9473.89Putative L-ascorbate peroxidase 6 OS=Arabidopsis thaliana OX=3702 GN=APX6 PE=2 S... [more]
Q1PER61.4e-3538.02L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis thaliana OX=3702 GN=APX2 PE=2... [more]
Q6ZJJ13.5e-3436.33Probable L-ascorbate peroxidase 4, peroxisomal OS=Oryza sativa subsp. japonica O... [more]
Q7XZP54.6e-3438.33L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=APX5 PE... [more]
Q01MI98.7e-3337.08Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica OX=39946 GN=APX3... [more]
Match NameE-valueIdentityDescription
XP_004138724.11.92e-243100.00putative L-ascorbate peroxidase 6 isoform X1 [Cucumis sativus] >XP_011649824.1 p... [more]
XP_008445223.13.48e-22594.67PREDICTED: putative L-ascorbate peroxidase 6 isoform X4 [Cucumis melo] >KAA00649... [more]
XP_038885450.11.06e-21991.42putative L-ascorbate peroxidase 6 isoform X1 [Benincasa hispida] >XP_038885451.1... [more]
XP_038885452.18.42e-21991.12putative L-ascorbate peroxidase 6 isoform X2 [Benincasa hispida][more]
XP_008445219.11.56e-19894.14PREDICTED: putative L-ascorbate peroxidase 6 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0LQ879.29e-244100.00L-ascorbate peroxidase OS=Cucumis sativus OX=3659 GN=Csa_2G381780 PE=3 SV=1[more]
A0A5A7V9T21.68e-22594.67L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3BBP41.68e-22594.67L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1[more]
A0A1S3BC457.57e-19994.14L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1[more]
A0A1S3BCW65.98e-19893.81L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103488313 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G32320.17.4e-9673.89ascorbate peroxidase 6 [more]
AT3G09640.11.0e-3638.02ascorbate peroxidase 2 [more]
AT3G09640.21.0e-3638.02ascorbate peroxidase 2 [more]
AT4G35970.13.3e-3538.33ascorbate peroxidase 5 [more]
AT4G35000.14.4e-3235.42ascorbate peroxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002016Haem peroxidasePRINTSPR00458PEROXIDASEcoord: 246..261
score: 48.77
coord: 201..213
score: 50.51
coord: 124..138
score: 47.46
coord: 183..200
score: 38.52
IPR002016Haem peroxidasePFAMPF00141peroxidasecoord: 112..314
e-value: 1.3E-39
score: 136.3
IPR002016Haem peroxidasePROSITEPS50873PEROXIDASE_4coord: 124..338
score: 19.02157
IPR002207Class I peroxidasePRINTSPR00459ASPEROXIDASEcoord: 104..124
score: 14.29
coord: 310..334
score: 38.62
coord: 182..200
score: 36.44
coord: 240..261
score: 45.63
coord: 201..213
score: 50.3
coord: 222..239
score: 26.28
coord: 142..152
score: 34.62
NoneNo IPR availableGENE3D1.10.520.10coord: 112..333
e-value: 1.5E-66
score: 226.0
NoneNo IPR availableGENE3D1.10.420.10Peroxidase, domain 2coord: 224..326
e-value: 1.5E-66
score: 226.0
NoneNo IPR availablePIRSRPIRSR600823-3PIRSR600823-3coord: 111..257
e-value: 3.3E-22
score: 75.9
NoneNo IPR availablePANTHERPTHR31356:SF8L-ASCORBATE PEROXIDASE 6-RELATEDcoord: 23..336
IPR044831Heme-binding peroxidase Ccp1-likePANTHERPTHR31356THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATEDcoord: 23..336
IPR019794Peroxidase, active sitePROSITEPS00436PEROXIDASE_2coord: 124..135
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 246..256
IPR010255Haem peroxidase superfamilySUPERFAMILY48113Heme-dependent peroxidasescoord: 115..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G023300.2CsGy2G023300.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0034599 cellular response to oxidative stress
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0005737 cytoplasm
molecular_function GO:0020037 heme binding
molecular_function GO:0016688 L-ascorbate peroxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004601 peroxidase activity