Cp4.1LG03g02300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionActin-depolymerizing factor
LocationCp4.1LG03: 655207 .. 657457 (-)
RNA-Seq ExpressionCp4.1LG03g02300
SyntenyCp4.1LG03g02300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATAAGAAAAAGGACCCAACAAGAAAACCACTATAAAAGCCAGTCTACATTTTTTTCTCATTTCTGAGCCCGCCTTCTTCATCATCTTCATCGGAGCTTCTTCTGTCCCCTCTCAAAATGGTGAGTTCTTCTGAAGTTTACTTACTTTCTGCTTTTATCCGTGGTCTGACGATCGTTTGGTTTTTGAGAAAGTAGGATTCTATTTCTAGTTTGCAACTTTGAAAATAACTACACTCTTGCGATCGGGAGTTTCTTGCAATTTGCGGAGTCTTTGAATCTTTGTATATAACTTTGAAGTCGTAATCCGCATGCAGTTTCTGTGGCGGGGAGTGAATGCGTTTTGTTCCCTAGGAGATCGGATTTGAGTTCTTCTGTGGTTGTTTCAACTAAACGAAACGTCATGTTCTTTGATTTATTGTCGACTTTGAGTGATCGAGTGGCCGTGGATCTATCACGATCTTCGTTTTTATTTTTTCACTTCTTGTTTTGTTACATTTGCAAATTTCTGTCAAGGATTGCAATTACAAGTTAATATTTGTATGTTCCCCCTTTTGAACCTTTTGAATTGCTATTAATCTTCTTATGCATGTTGTAAATGAGGGGGAAACTCTGTTAGGTTGTGATGCCTGCCTCTGATCCATATTTTGTTTGTTCCTTTGTTTTAAGTTTGGATGAAATCAATATCAACTATCAAGCTATGGATAAAAGCTTATGGGGGGAAATCTTATCCTGTCCATATTCTTATCTTGCGTGGCTGAGGAAGAGAATCCATACCATTTACTTGTTACATGGTTATCTTACTTTGACTTGGTGGTTCTCAAAGTGTAATAAAAAGGTTTTTAAGAAAATAAGCTTCTTGTTTGAGATCGTATGAATGTTTATGCATTGCTTTCTCTAAAATATTAGAAAAGAAAAAGTAGGAAGTCACTCTCAGTAGCTTCGTTAAGGGAACCTTTGCTTCAAAGGCTTCTTTGAAGCAAGGCATAAAGGCTGACCTTGGGGCTTAAGCGTGTCTTGGTGCGTGCCTCGGTGGGCTTCTTAAAACACTGATTTGACTAAACATGACTATAAATTGATGGCTATGATAAGGCTCTCAAGGGAGAAACAGAACAATTGTTGAAAATTTGAAAAATCTGATTAGATCAGAAAAATTCTCTTGTTCCCAATTTTATGCATGTCCAGTGTTGGGTTAAACATCAATCACCCCACTGCTTTGCTTTGTAGCCTTTGTTTGTATTGCTGTATTACCAATAACGTTTTATTTTCCAACTTTGTTCTTCCTTTTCAACTCTTTGTGTATGCATCTCTTCTGGACTTCAAAATTTTATTAAACAGCTTCGTTCTGGATTTCATAGGCCAATGCAGCATCGGGAATAGCCGTGAATGATGATTGCAAACTGAAGTTTTTGGAGCTCAAAGCAAAAAGAACCTATCGCTTTATTGTGTTTAAGATCGAAGAGAAGCAAAAGCAAGTTGTCGTAGAGAAAGTTGGCGAGCCAACACAAAGTTACGAGGACTTTGCTGCAAGCCTTCCTGCAGACGAATGTCGTTATGCTATCTATGATTTTGACTTTGTGACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTTATTGCTTGGTATGGCTGATATCTCCTTTACTGCATTCCTTTCATGTTTATCTTTCCAACCACCGCAGTCTTACGAAACACCATTGCTTGAAGGTCCCCTGATAATTCAAGAGTCAGAAGCAAAATGATTTATGCCAGCTCCAAAGATAGATTCAAGAGAGAACTGGATGGATTTCAGGTAGAGCTGCAAGCAACCGATCCGACTGAGATGGGGCTTGATGTCATTCGAAGCCGAGTTGGCTGAATGCTACATTAGCCAATTCAGGTGAACGGTATCAATATCCCTGCATATCTTCAGTTCGAGGTGAACGAGTGTCTTGGTCGTGGTTTTGTGGTATATAATGTGTTTTGAATTTATGTTCTAAATGGTCTTTTGTATGAGTTCGAGCTAAATAAGCCTCTTAGTTTTTCAACTATATATACAATTTCATCCTTTAGGGAGATATTGCAGCAGAATGCCATCAGATTCCAGCTGCTGTTCTACCTATGCTCCACAGATTTATATACAAGAGGATTGGATATCCGTACTTGAGCAAATATTGGTGTAATTTTCCTTTTAGTGTTCTTCTTTAGAAGCTGAGAAAAGTATTTATTTGCTTGGAAGTAATCGCCCTACTTTTTATATTGATTTTTTTTTT

mRNA sequence

TAATAAGAAAAAGGACCCAACAAGAAAACCACTATAAAAGCCAGTCTACATTTTTTTCTCATTTCTGAGCCCGCCTTCTTCATCATCTTCATCGGAGCTTCTTCTGTCCCCTCTCAAAATGGCCAATGCAGCATCGGGAATAGCCGTGAATGATGATTGCAAACTGAAGTTTTTGGAGCTCAAAGCAAAAAGAACCTATCGCTTTATTGTGTTTAAGATCGAAGAGAAGCAAAAGCAAGTTGTCGTAGAGAAAGTTGGCGAGCCAACACAAAGTTACGAGGACTTTGCTGCAAGCCTTCCTGCAGACGAATGTCGTTATGCTATCTATGATTTTGACTTTGTGACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTTATTGCTTGGTCCCCTGATAATTCAAGAGTCAGAAGCAAAATGATTTATGCCAGCTCCAAAGATAGATTCAAGAGAGAACTGGATGGATTTCAGGTAGAGCTGCAAGCAACCGATCCGACTGAGATGGGGCTTGATGTCATTCGAAGCCGAGTTGGCTGAATGCTACATTAGCCAATTCAGGTGAACGGTATCAATATCCCTGCATATCTTCAGTTCGAGGTGAACGAGTGTCTTGGTCGTGGTTTTGTGGTATATAATGTGTTTTGAATTTATGTTCTAAATGGTCTTTTGTATGAGTTCGAGCTAAATAAGCCTCTTAGTTTTTCAACTATATATACAATTTCATCCTTTAGGGAGATATTGCAGCAGAATGCCATCAGATTCCAGCTGCTGTTCTACCTATGCTCCACAGATTTATATACAAGAGGATTGGATATCCGTACTTGAGCAAATATTGGTGTAATTTTCCTTTTAGTGTTCTTCTTTAGAAGCTGAGAAAAGTATTTATTTGCTTGGAAGTAATCGCCCTACTTTTTATATTGATTTTTTTTTT

Coding sequence (CDS)

ATGGCCAATGCAGCATCGGGAATAGCCGTGAATGATGATTGCAAACTGAAGTTTTTGGAGCTCAAAGCAAAAAGAACCTATCGCTTTATTGTGTTTAAGATCGAAGAGAAGCAAAAGCAAGTTGTCGTAGAGAAAGTTGGCGAGCCAACACAAAGTTACGAGGACTTTGCTGCAAGCCTTCCTGCAGACGAATGTCGTTATGCTATCTATGATTTTGACTTTGTGACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTTATTGCTTGGTCCCCTGATAATTCAAGAGTCAGAAGCAAAATGATTTATGCCAGCTCCAAAGATAGATTCAAGAGAGAACTGGATGGATTTCAGGTAGAGCTGCAAGCAACCGATCCGACTGAGATGGGGCTTGATGTCATTCGAAGCCGAGTTGGCTGA

Protein sequence

MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRVG
Homology
BLAST of Cp4.1LG03g02300 vs. ExPASy Swiss-Prot
Match: Q9FVI1 (Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.6e-65
Identity = 121/139 (87.05%), Postives = 132/139 (94.96%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKLKFLELKAKRTYRFI++KIEEKQK+VVVEK+GEPT+SYEDF A L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYA+YDFDF+T+EN QKSRIFFIAWSPD +RVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 140
           LQATDPTEMGLDV RSR G
Sbjct: 121 LQATDPTEMGLDVFRSRAG 139

BLAST of Cp4.1LG03g02300 vs. ExPASy Swiss-Prot
Match: Q9FVI2 (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.3e-64
Identity = 120/137 (87.59%), Postives = 131/137 (95.62%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEK+GEPT+SYEDFAASL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           P +ECRYA+YDFDFVT ENCQKSRIFFIAW PD +RVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSR 138
           LQA DPTEMGLDVI+SR
Sbjct: 121 LQACDPTEMGLDVIQSR 137

BLAST of Cp4.1LG03g02300 vs. ExPASy Swiss-Prot
Match: Q9ZSK3 (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 1.5e-63
Identity = 119/138 (86.23%), Postives = 131/138 (94.93%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSRV 139
           LQATDPTEM LDV++SRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

BLAST of Cp4.1LG03g02300 vs. ExPASy Swiss-Prot
Match: Q0DLA3 (Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 PE=3 SV=2)

HSP 1 Score: 242.7 bits (618), Expect = 2.5e-63
Identity = 117/137 (85.40%), Postives = 131/137 (95.62%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+D+CKLKFLELKAKRTYRFI++KI+EK+K VVVEKVGEP  +Y+DFAASL
Sbjct: 1   MANAASGMAVDDECKLKFLELKAKRTYRFIIYKIDEKKKMVVVEKVGEPVLNYDDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PA+ECRYAI+D+DFVTEENCQKS+IFFIAWSPD SRVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PANECRYAIFDYDFVTEENCQKSKIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSR 138
           LQATDPTE+GLDVIR R
Sbjct: 121 LQATDPTEVGLDVIRGR 137

BLAST of Cp4.1LG03g02300 vs. ExPASy Swiss-Prot
Match: Q39250 (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 9.6e-63
Identity = 118/137 (86.13%), Postives = 129/137 (94.16%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSR 138
           LQATDPTEM LDV RSR
Sbjct: 121 LQATDPTEMDLDVFRSR 137

BLAST of Cp4.1LG03g02300 vs. NCBI nr
Match: XP_022935027.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023528602.1 actin-depolymerizing factor 2-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6580558.1 Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 277 bits (708), Expect = 8.00e-94
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. NCBI nr
Match: XP_022983563.1 (actin-depolymerizing factor 2-like [Cucurbita maxima])

HSP 1 Score: 275 bits (704), Expect = 3.26e-93
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT+ENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PADECRYAIYDFDFVTKENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. NCBI nr
Match: XP_038905295.1 (actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905297.1 actin-depolymerizing factor 2-like [Benincasa hispida])

HSP 1 Score: 275 bits (702), Expect = 6.58e-93
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGMAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           P+DECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. NCBI nr
Match: XP_023528601.1 (actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 275 bits (703), Expect = 2.15e-92
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 2   ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 61
           ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP
Sbjct: 47  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 106

Query: 62  ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 121
           ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL
Sbjct: 107 ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 166

Query: 122 QATDPTEMGLDVIRSRVG 139
           QATDPTEMGLDVIRSRVG
Sbjct: 167 QATDPTEMGLDVIRSRVG 184

BLAST of Cp4.1LG03g02300 vs. NCBI nr
Match: KAG7017313.1 (Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275 bits (703), Expect = 4.11e-92
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0

Query: 2   ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 61
           ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP
Sbjct: 66  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 125

Query: 62  ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 121
           ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL
Sbjct: 126 ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 185

Query: 122 QATDPTEMGLDVIRSRVG 139
           QATDPTEMGLDVIRSRVG
Sbjct: 186 QATDPTEMGLDVIRSRVG 203

BLAST of Cp4.1LG03g02300 vs. ExPASy TrEMBL
Match: A0A6J1F4F4 (actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442017 PE=3 SV=1)

HSP 1 Score: 277 bits (708), Expect = 3.87e-94
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. ExPASy TrEMBL
Match: A0A6J1J833 (actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482130 PE=3 SV=1)

HSP 1 Score: 275 bits (704), Expect = 1.58e-93
Identity = 138/139 (99.28%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT+ENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PADECRYAIYDFDFVTKENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. ExPASy TrEMBL
Match: A0A6J1L3B8 (actin-depolymerizing factor 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498729 PE=3 SV=1)

HSP 1 Score: 270 bits (691), Expect = 1.52e-91
Identity = 134/139 (96.40%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AVNDDCKLKFL LKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGMAVNDDCKLKFLALKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           P+DECRYA+YDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PSDECRYAVYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSR G
Sbjct: 121 LQATDPTEMGLDVIRSRAG 139

BLAST of Cp4.1LG03g02300 vs. ExPASy TrEMBL
Match: A0A6J1G2E2 (actin-depolymerizing factor 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450067 PE=3 SV=1)

HSP 1 Score: 270 bits (691), Expect = 1.52e-91
Identity = 134/139 (96.40%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AVNDDCKLKFL LKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGMAVNDDCKLKFLALKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           P+DECRYA+YDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PSDECRYAVYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSR G
Sbjct: 121 LQATDPTEMGLDVIRSRAG 139

BLAST of Cp4.1LG03g02300 vs. ExPASy TrEMBL
Match: A0A0A0LFL5 (ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734880 PE=3 SV=1)

HSP 1 Score: 270 bits (690), Expect = 2.16e-91
Identity = 135/139 (97.12%), Postives = 138/139 (99.28%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASGIAV+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFA SL
Sbjct: 1   MANAASGIAVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAKSL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           P+DECRYAIYDFDFVTEENCQKSRIFFIAWSPD+SRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDSSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 121 LQATDPTEMGLDVIRSRVG 139
           LQATDPTEMGLDVIRSRVG
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

BLAST of Cp4.1LG03g02300 vs. TAIR 10
Match: AT5G59890.1 (actin depolymerizing factor 4 )

HSP 1 Score: 243.4 bits (620), Expect = 1.1e-64
Identity = 119/138 (86.23%), Postives = 131/138 (94.93%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSRV 139
           LQATDPTEM LDV++SRV
Sbjct: 121 LQATDPTEMDLDVLKSRV 138

BLAST of Cp4.1LG03g02300 vs. TAIR 10
Match: AT3G46010.1 (actin depolymerizing factor 1 )

HSP 1 Score: 240.7 bits (613), Expect = 6.8e-64
Identity = 118/137 (86.13%), Postives = 129/137 (94.16%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PADECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSR 138
           LQATDPTEM LDV RSR
Sbjct: 121 LQATDPTEMDLDVFRSR 137

BLAST of Cp4.1LG03g02300 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 238.8 bits (608), Expect = 2.6e-63
Identity = 117/136 (86.03%), Postives = 128/136 (94.12%), Query Frame = 0

Query: 2   ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 61
           ANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA LP
Sbjct: 13  ANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP 72

Query: 62  ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 121
           ADECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVEL
Sbjct: 73  ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVEL 132

Query: 122 QATDPTEMGLDVIRSR 138
           QATDPTEM LDV RSR
Sbjct: 133 QATDPTEMDLDVFRSR 148

BLAST of Cp4.1LG03g02300 vs. TAIR 10
Match: AT3G46000.1 (actin depolymerizing factor 2 )

HSP 1 Score: 232.3 bits (591), Expect = 2.4e-61
Identity = 113/137 (82.48%), Postives = 127/137 (92.70%), Query Frame = 0

Query: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60
           MANAASG+AV+DDCKLKF+ELKAKRT+R IV+KIE+  KQV+VEK+GEP QSY+DFAASL
Sbjct: 1   MANAASGMAVHDDCKLKFMELKAKRTFRTIVYKIED--KQVIVEKLGEPEQSYDDFAASL 60

Query: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120
           PAD+CRY IYDFDFVT ENCQKS+IFFIAWSPD ++VR KMIYASSKDRFKRELDG QVE
Sbjct: 61  PADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPTEMGLDVIRSR 138
           LQATDPTEMGLDV +SR
Sbjct: 121 LQATDPTEMGLDVFKSR 135

BLAST of Cp4.1LG03g02300 vs. TAIR 10
Match: AT5G59890.2 (actin depolymerizing factor 4 )

HSP 1 Score: 230.7 bits (587), Expect = 7.1e-61
Identity = 112/131 (85.50%), Postives = 124/131 (94.66%), Query Frame = 0

Query: 8   IAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRY 67
           +AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASLPADECRY
Sbjct: 1   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRY 60

Query: 68  AIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 127
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 61  AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 120

Query: 128 EMGLDVIRSRV 139
           EM LDV++SRV
Sbjct: 121 EMDLDVLKSRV 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVI11.6e-6587.05Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1[more]
Q9FVI21.3e-6487.59Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1[more]
Q9ZSK31.5e-6386.23Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2[more]
Q0DLA32.5e-6385.40Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 P... [more]
Q392509.6e-6386.13Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022935027.18.00e-94100.00actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023528602.1 actin-de... [more]
XP_022983563.13.26e-9399.28actin-depolymerizing factor 2-like [Cucurbita maxima][more]
XP_038905295.16.58e-9398.56actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-dep... [more]
XP_023528601.12.15e-92100.00actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7017313.14.11e-92100.00Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
Match NameE-valueIdentityDescription
A0A6J1F4F43.87e-94100.00actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442017... [more]
A0A6J1J8331.58e-9399.28actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482130 P... [more]
A0A6J1L3B81.52e-9196.40actin-depolymerizing factor 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G2E21.52e-9196.40actin-depolymerizing factor 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0LFL52.16e-9197.12ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734880 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G59890.11.1e-6486.23actin depolymerizing factor 4 [more]
AT3G46010.16.8e-6486.13actin depolymerizing factor 1 [more]
AT3G46010.22.6e-6386.03actin depolymerizing factor 1 [more]
AT3G46000.12.4e-6182.48actin depolymerizing factor 2 [more]
AT5G59890.27.1e-6185.50actin depolymerizing factor 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 12..139
e-value: 3.5E-60
score: 215.9
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 14..136
e-value: 3.0E-37
score: 127.2
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 5..139
score: 39.956787
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 1..139
e-value: 1.2E-53
score: 183.2
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 1..137
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 6..138
e-value: 1.57575E-66
score: 195.853
NoneNo IPR availablePANTHERPTHR11913:SF74ACTIN-DEPOLYMERIZING FACTOR 2-LIKEcoord: 1..137
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 3..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02300.1Cp4.1LG03g02300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051015 actin filament binding
molecular_function GO:0003779 actin binding