CsGy3G035160 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G035160
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	3'-5' exonuclease domain-containing protein
Location	Gy14Chr3: 33725021 .. 33725809 (+)
RNA-Seq Expression	CsGy3G035160
Synteny	CsGy3G035160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTGAGTCAAATCTCGCAAGTAATGTTTAGGGTTTTTGATTTTGGTTGTGAAGATCCTTCTAGGGTGTTCGGTTTTCTTTTCATGAGTTCTGTATAGGATTAGCAAATGGAATATTACGTTCATAACTGTTCTTCTGCTGGATCCATGTTCATTGGAGTAA

mRNA sequence

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGATTAGCAAATGGAATATTACGTTCATAACTGTTCTTCTGCTGGATCCATGTTCATTGGAGTAA

Coding sequence (CDS)

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGATTAGCAAATGGAATATTACGTTCATAACTGTTCTTCTGCTGGATCCATGTTCATTGGAGTAA

Protein sequence

MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRISKWNITFITVLLLDPCSLE*

Homology

[Close]

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.0e-21
Identity = 59/171 (34.50%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDVEWRPNFDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLD+EWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 TPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
            PQSL   +ED T   VG+GID D +KL++DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRISK 210
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + ++ K
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 2.8e-19
Identity = 54/150 (36.00%), Postives = 84/150 (56.00%), Query Frame = 0


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQLCVGR-RCLIFQLIHASETPQSLIDFLEDDTFTFVG 117
           VVG D+EW P ++R     VA +QLCV   +C +F +   S  PQ L   LE+      G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 118 VGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  D+D+ + N V+L ++A  +++  E  T  L +L + +LG+++ K +++
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 178 KLSRWDRQWLNPAQILYATVDAFLSFEIGR 207
           + S W +  L   Q LYA  DA+  F I R
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYR 225

[Close]

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 92.0 bits (227), Expect = 9.0e-18
Identity = 51/143 (35.66%), Postives = 81/143 (56.64%), Query Frame = 0


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQLCVGR-RCLIFQLIHASETPQSLIDFLEDDTFTFVG 117
           VVG D+EW P +    R+ VA +QLCV   +C +F +   S  PQ L   LE+ +    G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 118 VGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  D+D+ + + V+L ++A ++++  E  T  L  L + VLG+++ K +++
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVANEKLKCAE--TWSLNGLVKHVLGKQLLKDKSI 191

Query: 178 KLSRWDRQWLNPAQILYATVDAF 200
           + S W    L   Q LYA  DA+
Sbjct: 192 RCSNWSNFPLTEDQKLYAATDAY 212

[Close]

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.2e-16
Identity = 59/164 (35.98%), Postives = 81/164 (49.39%), Query Frame = 0


Query: 42  WISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVATLQLCVGR-RCLIFQLIHASETP 101
           W+   L+ H +     V+G D EW        R PVA LQL   R  C +F+L H  + P
Sbjct: 65  WVLNELKNHCQTFK--VLGFDCEWITVGGS--RRPVALLQLSSHRGLCALFRLCHMKQIP 124

Query: 102 QSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKT 161
           Q L + LEDD+   VGV    D +KL +DY + VA+T+DLR L        E    GL  
Sbjct: 125 QDLRELLEDDSVIKVGVAPQEDAMKLSHDYGVGVASTLDLRFLCVMAGHKPE----GLGK 184

Query: 162 LGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEI 205
           L +  L   +DK   +  S W+ + L P Q+ YA  DA ++  I
Sbjct: 185 LSKTHLNYTLDKHWRLACSNWEAKTLEPKQLDYAANDALMAVAI 220

[Close]

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-15
Identity = 46/143 (32.17%), Postives = 78/143 (54.55%), Query Frame = 0


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQLCVG-RRCLIFQLIHASETPQSLIDFLEDDTFTFVG 117
           V+G D+EW P + +     VA +Q+CV  ++C +F +   +  P+ L   LED++   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 118 VGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL +DY+L +   ++L E+A  +++  E  T     L + +   ++ K ++ 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWT--FNGLIKHLFKEQLYKRKSY 191

Query: 178 KLSRWDRQWLNPAQILYATVDAF 200
           + S WD   L   Q LYA  DA+
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAY 212

[Close]

BLAST of CsGy3G035160 vs. NCBI nr
Match: KAE8651170.1 (hypothetical protein Csa_000755 [Cucumis sativus])

HSP 1 Score: 461 bits (1185), Expect = 1.14e-163
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGRISKWNITFITVLLLDPCSLE 226
           WDRQWLNPAQILYATVDAFLSFEIGRISKWNITFITVLLLDPCSLE
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRISKWNITFITVLLLDPCSLE 226

[Close]

BLAST of CsGy3G035160 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 420 bits (1080), Expect = 6.53e-148
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206
           WDRQWLNPAQILYATVDAFLSFEIGR
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206

[Close]

BLAST of CsGy3G035160 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 355 bits (910), Expect = 5.30e-122
Identity = 172/206 (83.50%), Postives = 188/206 (91.26%), Query Frame = 0


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MA L+ISDRQIPLNSHDYYDITFD+D ILTL+TA  S+VDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LD+EWRPNF+R +R PVATLQLCVGRRCLIFQLIH SE P+SLIDFLEDDTFTFVGVGID
Sbjct: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
            DV +LYNDYDL VANTVDLRELAA+EM+ D+ + AGLK LGREVLGREIDKPRNV LS+
Sbjct: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206
           WDRQ L+P Q+LYATVDAFLSFEIGR
Sbjct: 181 WDRQCLSPGQVLYATVDAFLSFEIGR 206

[Close]

BLAST of CsGy3G035160 vs. NCBI nr
Match: XP_042939990.1 (Werner Syndrome-like exonuclease [Carya illinoinensis] >KAG2687445.1 hypothetical protein I3760_09G051400 [Carya illinoinensis] >KAG6694513.1 hypothetical protein I3842_09G051700 [Carya illinoinensis])

HSP 1 Score: 279 bits (713), Expect = 5.33e-92
Identity = 131/204 (64.22%), Postives = 166/204 (81.37%), Query Frame = 0


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++I D ++P ++H+ YD++FD D I TLVT+SPS+VD WI +I RIHRRRL+RL+VGLD+
Sbjct: 3   ISIFDHELPFDTHNLYDVSFDSDRIQTLVTSSPSVVDSWIFDIYRIHRRRLNRLIVGLDL 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F+RH +NPVATLQLCVGRRCLIFQLIHA+  P+SL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHATYIPESLVDFLGQTNFTFVGVGIKSDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL +DY+L VA  VDLR LA +E+   + R AGLK L  EVLG++I+KPRN+K+SRWD 
Sbjct: 123 EKLLDDYELEVACVVDLRLLAVEELGKMQLRNAGLKQLAWEVLGKQIEKPRNIKMSRWDN 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRI 207
           +WL  AQI YA +DAFLSFEIGR+
Sbjct: 183 EWLTRAQIQYACLDAFLSFEIGRL 206

[Close]

BLAST of CsGy3G035160 vs. NCBI nr
Match: XP_018843659.1 (Werner Syndrome-like exonuclease [Juglans regia])

HSP 1 Score: 278 bits (711), Expect = 1.07e-91
Identity = 132/203 (65.02%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++I D ++P ++H+ YD++FD D+I TL+T SPS+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F+RH +NPVATLQLCVGRRCLIFQLIHA   PQSL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL +DY L VA  VDLR LA +E+ + + R AGLK L  EVLG+ I+KPRN+ +SRWD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 QWLNPAQILYATVDAFLSFEIGR 206
           +WL  AQ+ YA +DAFLSFEIGR
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGR 205

[Close]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 3.16e-148
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206
           WDRQWLNPAQILYATVDAFLSFEIGR
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206

[Close]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL
Match: A0A2I4GID7 (Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 SV=1)

HSP 1 Score: 278 bits (711), Expect = 5.20e-92
Identity = 132/203 (65.02%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++I D ++P ++H+ YD++FD D+I TL+T SPS+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F+RH +NPVATLQLCVGRRCLIFQLIHA   PQSL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL +DY L VA  VDLR LA +E+ + + R AGLK L  EVLG+ I+KPRN+ +SRWD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 QWLNPAQILYATVDAFLSFEIGR 206
           +WL  AQ+ YA +DAFLSFEIGR
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGR 205

[Close]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 277 bits (709), Expect = 7.44e-92
Identity = 133/193 (68.91%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 14  NSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHF 73
           NSH+ YDITFD D+I T +T+SP MVD WIS ILR HRRRLHRL+VGLDVEWRPNF+R  
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  RNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLN 133
           RNP ATLQLCVGRRCLIFQLIHA E P SL+ FL D+TFTFVGVGID D  KL++DYDL 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILY 193
           V N VDLR +A D     + R AGLK L RE+LGREI KP+ V +SRWD ++L  AQ+ Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGR 206
           A++DAFLSFEIGR
Sbjct: 185 ASIDAFLSFEIGR 197

[Close]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL
Match: A0A251SDD2 (Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr15g0495511 PE=4 SV=1)

HSP 1 Score: 275 bits (704), Expect = 6.45e-91
Identity = 127/204 (62.25%), Postives = 164/204 (80.39%), Query Frame = 0


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++ISD Q+P N+HDY+D+TF +D ILTLVT +PS VDMWISEI RIHR RLH LVVGLD+
Sbjct: 3   ISISDHQVPFNTHDYFDVTFSNDTILTLVTHTPSFVDMWISEIERIHRHRLHSLVVGLDI 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F+R+ +NPVATLQLCVGRRCLIFQL++A   PQSL++FL +  +TF GVGI++DV
Sbjct: 63  EWRPSFNRNIQNPVATLQLCVGRRCLIFQLLYAPYIPQSLLNFLRNPNYTFTGVGIESDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL  DY+L V    D+R LAA+     EF+ AG+K+L  +VLG+E++KP+ V +S+WD+
Sbjct: 123 EKLVEDYNLGVERMEDVRTLAAEAYGVREFKNAGIKSLAEQVLGKEVNKPKWVTMSKWDQ 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRI 207
            WLNP Q+ YA VDAFLSFEIGR+
Sbjct: 183 PWLNPDQVQYACVDAFLSFEIGRV 206

[Close]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL
Match: A0A6A1WBZ0 (Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 PE=4 SV=1)

HSP 1 Score: 273 bits (699), Expect = 3.59e-90
Identity = 133/206 (64.56%), Postives = 164/206 (79.61%), Query Frame = 0


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MA+++I D Q+P N+++ Y ++ D D++LTLVT SPSMVD WIS+I RIH RRLHRLVVG
Sbjct: 1   MASISIFDHQLPDNTYNLYVVSLDSDDVLTLVTHSPSMVDSWISDIYRIHSRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRP+F+RH +NPVATLQLCVGRRCLIFQ++ A   P SLI+FL D +FTFVGVGI+
Sbjct: 61  LDVEWRPSFNRHVQNPVATLQLCVGRRCLIFQIVRAPYFPDSLIEFLGDGSFTFVGVGIE 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           +DV KL +DY+L VAN VDLR+L  +E    E R AGLK L  EVLG++I KPR V LSR
Sbjct: 121 SDVEKLLDDYELEVANVVDLRDLVEEEWGIRELRNAGLKQLAWEVLGKDIQKPRRVTLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206
           WD + L+ AQ+ YA +DAFLSFEIGR
Sbjct: 181 WDNERLSHAQVQYACLDAFLSFEIGR 206

[Close]

BLAST of CsGy3G035160 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 105.1 bits (261), Expect = 7.3e-23
Identity = 59/171 (34.50%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDVEWRPNFDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLD+EWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 TPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
            PQSL   +ED T   VG+GID D +KL++DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRISK 210
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + ++ K
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLK 279

[Close]

BLAST of CsGy3G035160 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.6e-22
Identity = 67/209 (32.06%), Postives = 97/209 (46.41%), Query Frame = 0


Query: 19  YDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPN---------- 78
           Y + F  +E + +VT  PS++  WI ++L  +R   H LVVG+ V+W P+          
Sbjct: 17  YLVDFFGEEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVR 76

Query: 79  ----------------FDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTF 138
                             R F  P  TLQLCVG RC+I QL H    PQ L +FL D  +
Sbjct: 77  YRSDSPPVRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDY 136

Query: 139 TFVGVGIDNDVLKL-YNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-I 198
           TFVG+    D  KL  + ++L +A  +DLR+  +D       +    + +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLKRSKHELEIAVLLDLRKFVSDS-SGRTMKRCSFEKIVEENLGHSGV 196

Query: 199 DKPRNVKLSRWDRQWLNPAQILYATVDAF 200
              R V  S W    L   QIL A++D +
Sbjct: 197 RLDRKVSRSDWRVYDLRYVQILQASIDVY 224

[Close]

BLAST of CsGy3G035160 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 102.4 bits (254), Expect = 4.7e-22
Identity = 58/166 (34.94%), Postives = 94/166 (56.63%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDVEWRPNFDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLD+EWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 TPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
            PQSL   +ED T   VG+GID D +KL++DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEI 205
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ +
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]

BLAST of CsGy3G035160 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 5.3e-21
Identity = 67/211 (31.75%), Postives = 99/211 (46.92%), Query Frame = 0


Query: 14  NSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEW-------- 73
           N+H  Y + F  DE +  VT   S++  WI  +L  +R   H LVVG+ V+W        
Sbjct: 13  NTHLEYSVDFFGDEFIVTVTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 72

Query: 74  -RPN--------FDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVG 133
            RPN           +  NP   LQLCVG RCLI QL +  + P +L  FL D   TFVG
Sbjct: 73  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 132

Query: 134 VGIDNDVLKLYN-DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-IDKPR 193
           V    D  KL    + L +   +D+R    D       R +  + +  E +G + +    
Sbjct: 133 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSW-GRSMRRSSFEEIVEECMGYQGVMLDP 192

Query: 194 NVKLSRWDRQWLNPAQILYATVDAFLSFEIG 206
            + +S W    L+  QIL A++DA++  ++G
Sbjct: 193 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of CsGy3G035160 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 6.9e-21
Identity = 66/193 (34.20%), Postives = 96/193 (49.74%), Query Frame = 0


Query: 16  HDYYDITFDDDEILTLVTASPSMVDMWISEILRIHR-RRLHRLVVGLDVEWRPNFDRHFR 75
           H+   I F  + ++  VT + S +  WI  I    R R  H LVVGLDV+W P       
Sbjct: 14  HENRYIDFFGERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS---D 73

Query: 76  NPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYN-DYDLN 135
            P   LQLCVG RCLI QL H    P+ L  FLED+T TFVGV    D  KL    + L 
Sbjct: 74  PPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLE 133

Query: 136 VANTVDLRELAADEMQSDEFRTAGLKTLGREVLG-REIDKPRNVKLSRWDRQWLNPAQIL 195
           +   +D+R      + +  F     + +  E LG + + K + + +S W  + L+  QI+
Sbjct: 134 IWRLLDIRHYLPTRLLNSSF-----EKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIV 193

Query: 196 YATVDAFLSFEIG 206
            A+ D ++  ++G
Sbjct: 194 QASDDVYVCCKLG 198

The following BLAST results are available for this feature:

BLAST of CsGy3G035160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	1.0e-21	34.50	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	2.8e-19	36.00	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	9.0e-18	35.66	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9VGN7	2.2e-16	35.98	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
O93530	1.9e-15	32.17	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]

BLAST of CsGy3G035160 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651170.1	1.14e-163	100.00	hypothetical protein Csa_000755 [Cucumis sativus]	[more]
XP_004136771.1	6.53e-148	100.00	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
XP_038906336.1	5.30e-122	83.50	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
XP_042939990.1	5.33e-92	64.22	Werner Syndrome-like exonuclease [Carya illinoinensis] >KAG2687445.1 hypothetica...	[more]
XP_018843659.1	1.07e-91	65.02	Werner Syndrome-like exonuclease [Juglans regia]	[more]

BLAST of CsGy3G035160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5	3.16e-148	100.00	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A2I4GID7	5.20e-92	65.02	Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 ...	[more]
A0A6J1D121	7.44e-92	68.91	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A251SDD2	6.45e-91	62.25	Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4...	[more]
A0A6A1WBZ0	3.59e-90	64.56	Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 ...	[more]

BLAST of CsGy3G035160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.2	7.3e-23	34.50	Werner syndrome-like exonuclease	[more]
AT3G12430.1	3.6e-22	32.06	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.1	4.7e-22	34.94	Werner syndrome-like exonuclease	[more]
AT3G12410.1	5.3e-21	31.75	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	6.9e-21	34.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 50..204 e-value: 9.5E-14 score: 51.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 17..207 e-value: 5.8E-59 score: 201.0
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..207
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..207
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..207 e-value: 2.31682E-62 score: 190.482
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 41..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G035160.1	CsGy3G035160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding