CsGy7G020730 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy7G020730
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionSubtilisin-like protease SBT1.4
LocationGy14Chr7: 23049982 .. 23052630 (+)
RNA-Seq ExpressionCsGy7G020730
SyntenyCsGy7G020730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTAATTAGGTTTATTATTTTATTTATTTATTTATTACATCTTCACTTTCCTTCCCACTTCTTATCCCCAAAATAATTGAAGAACAGAGTTTCTTCTTCAATGGCGAATTTCCATCTCCCTTCTTCTTCATTTTTCTTCTTCTTCCTTTTCTGTTTCTTCCCTCTGATTTTCTCTCGATCGCTGGAAAATCAAGAAACATACATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTCTCCTCTCATCATCACTGGCACTCTTCCATTCTTGAATCACTCTCTTCTTCACCTCATCCCACCAAACTTCTTTACAACTACGAGCGTGCCGCTAATGGATTCTCCGCACGAATCACGACTGTTCAGGCCGAGGAGCTGCGTCGTGTTCCAGGTATTATTTCGGTGATTCCTGATCAGATCCGTCAGCTTCATACCACTCGGACGCCTCATTTCTTGGGCTTGGCGGATAACCTAGGGCTCTGGGCTGACACGAATTATGCCGATGATGTGATTATTGGTGTTCTTGATACTGGAATTTGGCCGGAGAGACCAAGTTTTTCTGATGAAGGTTTGTCTCCGGTTCCGGCAAGATGGAAAGGTACGTGCGATACTGGGGAGGGAGTTTCGGCTTTTGCTTGCAATCGGAAGATTATTGGTGCAAGAGCGTATTTTTACGGATATGAGTCTAATTTAAGAGGATCGCTGAAAGTTTCTTCGGATTTCAAATCTGCTAGAGATACGGAGGGCCATGGAACTCATACGGCTTCAACGGCTGCAGGTTCGTTCGTTAACAATGCTAGTTTTTTCCAGTACGCTCGTGGTGAAGCCAGAGGTATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGCTGTTACGACTCTGACATACTTGCCGCTATGGATCAGGCGATTTCCGATGGCGTCGACGTCATCTCTCTCTCAGTAGGATCCTCCGGTCGTGCCCCTGCGTATTATCGAGACTCCATTGCAATCGGCGCGTTTGGCGCAATGCAGCACGGCGTCGTTGTTTCTTGCTCCGCCGGTAACTCCGGTCCTGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTTGGTGCCTCCACAATCGATAGAGAGTTTCTTGCCGATGTCATTCTTGGAGACGGTAGGGTTTTTAGCGGCGTGTCGCTTTACTCCGGTGATCCTCTTGGCGATTCCAAGCTTCAGTTGGTCTATGGTGGAGATTGCGGTAGCCGGTATTGTTATTCTGGATCCCTTGATTCGTCGAAAGTTGCTGGAAAAATTGTCGTGTGCGATCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAATCCGCCGGCGGTCTTGGTATGGTACTAGCGAACACGGAGGAAAACGGCGAAGAACTCTTAGCAGACTCCCATCTTATTCCAGGAACAATGGTCGGTGCAATCGCCGGCAACAAGCTTCGAGACTACATTCATACAGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCGATTCACCGCCAGCTCCTAGAGTCGCTGCCTTCTCCAGCCGGGGTCCGAACTACAGGACTGCAGAGATTCTAAAACCAGACGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCGGTCTAAACATCGATCCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCATGTCAGTGGCGTCGCCGCCTTACTCCGAAAGGCTTTTCCGACGTGGTCACCTGCTGCAATCAAATCTGCTCTGATCACCACTTCTTATTCTTTGGACAGTTCCGGCAAACCAATAAAAGATCTCTCCACAAGCGAAGAATCAAATCCATTCGTCCATGGCGCCGGCCATATCAATCCCAACCAAGCTCTGAATCCAGGCTTGATTTATGATCTCACTCCTCAAGATTACGTTTCATTTCTCTGCTCAATTGGTTACGATTCTAAACAAATAGCTGTTTTCGTGAAGGGTTCTTCATACTTCCAACTTTGTGAACACAAGCTGACCAACCCCGGCAACCTGAATTACCCATCTTTCTCTGTCGTGTTCGACGAGGAGGAGGTGGTGAAATACACAAGAACAGTGACAAATGTAGGAGATGAAACTGAAGTTGTTTATGAAGTGAAAGTAGAAGCTCCACAAGGCGTTGTAATCAGTGTTGTTCCAAACAAGCTGGAGTTCAATAAAGAGAAGACAACACAGTCTTATGAGATCACATTCACCAAAATCAATGGATTCAAGGAATCAGCAAGCTTTGGATCAATTCAATGGGGTGATGGAATCCACAGTGTTAGGAGTCCAATCGCTGTGTCCTTTAAGACTGGAGGATCCATAGCTTCGATGTAAGTTGGGTATTTGTTGATTTTTGAGTTTTGAATTAGGATATTTAATAGCGCAATACTTTGGTGATTTCCGCCATTGTTGGTGGCTACCAAAGCTCCTCTTTCTGTACAGTACAATGTGGAGGAGATTGCAACTGTTCATTTGGAATACTCCTCCAATGGAATAATAACACTCAACTTTATAATAATATTCATTAATGGTTGCTTCATCTCTTAATTTGAAACCCAATGGCTACA

mRNA sequence

TGTAATTAGGTTTATTATTTTATTTATTTATTTATTACATCTTCACTTTCCTTCCCACTTCTTATCCCCAAAATAATTGAAGAACAGAGTTTCTTCTTCAATGGCGAATTTCCATCTCCCTTCTTCTTCATTTTTCTTCTTCTTCCTTTTCTGTTTCTTCCCTCTGATTTTCTCTCGATCGCTGGAAAATCAAGAAACATACATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTCTCCTCTCATCATCACTGGCACTCTTCCATTCTTGAATCACTCTCTTCTTCACCTCATCCCACCAAACTTCTTTACAACTACGAGCGTGCCGCTAATGGATTCTCCGCACGAATCACGACTGTTCAGGCCGAGGAGCTGCGTCGTGTTCCAGGTATTATTTCGGTGATTCCTGATCAGATCCGTCAGCTTCATACCACTCGGACGCCTCATTTCTTGGGCTTGGCGGATAACCTAGGGCTCTGGGCTGACACGAATTATGCCGATGATGTGATTATTGGTGTTCTTGATACTGGAATTTGGCCGGAGAGACCAAGTTTTTCTGATGAAGGTTTGTCTCCGGTTCCGGCAAGATGGAAAGGTACGTGCGATACTGGGGAGGGAGTTTCGGCTTTTGCTTGCAATCGGAAGATTATTGGTGCAAGAGCGTATTTTTACGGATATGAGTCTAATTTAAGAGGATCGCTGAAAGTTTCTTCGGATTTCAAATCTGCTAGAGATACGGAGGGCCATGGAACTCATACGGCTTCAACGGCTGCAGGTTCGTTCGTTAACAATGCTAGTTTTTTCCAGTACGCTCGTGGTGAAGCCAGAGGTATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGCTGTTACGACTCTGACATACTTGCCGCTATGGATCAGGCGATTTCCGATGGCGTCGACGTCATCTCTCTCTCAGTAGGATCCTCCGGTCGTGCCCCTGCGTATTATCGAGACTCCATTGCAATCGGCGCGTTTGGCGCAATGCAGCACGGCGTCGTTGTTTCTTGCTCCGCCGGTAACTCCGGTCCTGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTTGGTGCCTCCACAATCGATAGAGAGTTTCTTGCCGATGTCATTCTTGGAGACGGTAGGGTTTTTAGCGGCGTGTCGCTTTACTCCGGTGATCCTCTTGGCGATTCCAAGCTTCAGTTGGTCTATGGTGGAGATTGCGGTAGCCGGTATTGTTATTCTGGATCCCTTGATTCGTCGAAAGTTGCTGGAAAAATTGTCGTGTGCGATCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAATCCGCCGGCGGTCTTGGTATGGTACTAGCGAACACGGAGGAAAACGGCGAAGAACTCTTAGCAGACTCCCATCTTATTCCAGGAACAATGGTCGGTGCAATCGCCGGCAACAAGCTTCGAGACTACATTCATACAGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCGATTCACCGCCAGCTCCTAGAGTCGCTGCCTTCTCCAGCCGGGGTCCGAACTACAGGACTGCAGAGATTCTAAAACCAGACGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCGGTCTAAACATCGATCCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCATGTCAGTGGCGTCGCCGCCTTACTCCGAAAGGCTTTTCCGACGTGGTCACCTGCTGCAATCAAATCTGCTCTGATCACCACTTCTTATTCTTTGGACAGTTCCGGCAAACCAATAAAAGATCTCTCCACAAGCGAAGAATCAAATCCATTCGTCCATGGCGCCGGCCATATCAATCCCAACCAAGCTCTGAATCCAGGCTTGATTTATGATCTCACTCCTCAAGATTACGTTTCATTTCTCTGCTCAATTGGTTACGATTCTAAACAAATAGCTGTTTTCGTGAAGGGTTCTTCATACTTCCAACTTTGTGAACACAAGCTGACCAACCCCGGCAACCTGAATTACCCATCTTTCTCTGTCGTGTTCGACGAGGAGGAGGTGGTGAAATACACAAGAACAGTGACAAATGTAGGAGATGAAACTGAAGTTGTTTATGAAGTGAAAGTAGAAGCTCCACAAGGCGTTGTAATCAGTGTTGTTCCAAACAAGCTGGAGTTCAATAAAGAGAAGACAACACAGTCTTATGAGATCACATTCACCAAAATCAATGGATTCAAGGAATCAGCAAGCTTTGGATCAATTCAATGGGGTGATGGAATCCACAGTGTTAGGAGTCCAATCGCTGTGTCCTTTAAGACTGGAGGATCCATAGCTTCGATGTAAGTTGGGTATTTGTTGATTTTTGAGTTTTGAATTAGGATATTTAATAGCGCAATACTTTGGTGATTTCCGCCATTGTTGGTGGCTACCAAAGCTCCTCTTTCTGTACAGTACAATGTGGAGGAGATTGCAACTGTTCATTTGGAATACTCCTCCAATGGAATAATAACACTCAACTTTATAATAATATTCATTAATGGTTGCTTCATCTCTTAATTTGAAACCCAATGGCTACA

Coding sequence (CDS)

ATGGCGAATTTCCATCTCCCTTCTTCTTCATTTTTCTTCTTCTTCCTTTTCTGTTTCTTCCCTCTGATTTTCTCTCGATCGCTGGAAAATCAAGAAACATACATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTCTCCTCTCATCATCACTGGCACTCTTCCATTCTTGAATCACTCTCTTCTTCACCTCATCCCACCAAACTTCTTTACAACTACGAGCGTGCCGCTAATGGATTCTCCGCACGAATCACGACTGTTCAGGCCGAGGAGCTGCGTCGTGTTCCAGGTATTATTTCGGTGATTCCTGATCAGATCCGTCAGCTTCATACCACTCGGACGCCTCATTTCTTGGGCTTGGCGGATAACCTAGGGCTCTGGGCTGACACGAATTATGCCGATGATGTGATTATTGGTGTTCTTGATACTGGAATTTGGCCGGAGAGACCAAGTTTTTCTGATGAAGGTTTGTCTCCGGTTCCGGCAAGATGGAAAGGTACGTGCGATACTGGGGAGGGAGTTTCGGCTTTTGCTTGCAATCGGAAGATTATTGGTGCAAGAGCGTATTTTTACGGATATGAGTCTAATTTAAGAGGATCGCTGAAAGTTTCTTCGGATTTCAAATCTGCTAGAGATACGGAGGGCCATGGAACTCATACGGCTTCAACGGCTGCAGGTTCGTTCGTTAACAATGCTAGTTTTTTCCAGTACGCTCGTGGTGAAGCCAGAGGTATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGCTGTTACGACTCTGACATACTTGCCGCTATGGATCAGGCGATTTCCGATGGCGTCGACGTCATCTCTCTCTCAGTAGGATCCTCCGGTCGTGCCCCTGCGTATTATCGAGACTCCATTGCAATCGGCGCGTTTGGCGCAATGCAGCACGGCGTCGTTGTTTCTTGCTCCGCCGGTAACTCCGGTCCTGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTTGGTGCCTCCACAATCGATAGAGAGTTTCTTGCCGATGTCATTCTTGGAGACGGTAGGGTTTTTAGCGGCGTGTCGCTTTACTCCGGTGATCCTCTTGGCGATTCCAAGCTTCAGTTGGTCTATGGTGGAGATTGCGGTAGCCGGTATTGTTATTCTGGATCCCTTGATTCGTCGAAAGTTGCTGGAAAAATTGTCGTGTGCGATCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAATCCGCCGGCGGTCTTGGTATGGTACTAGCGAACACGGAGGAAAACGGCGAAGAACTCTTAGCAGACTCCCATCTTATTCCAGGAACAATGGTCGGTGCAATCGCCGGCAACAAGCTTCGAGACTACATTCATACAGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCGATTCACCGCCAGCTCCTAGAGTCGCTGCCTTCTCCAGCCGGGGTCCGAACTACAGGACTGCAGAGATTCTAAAACCAGACGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCGGTCTAAACATCGATCCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCATGTCAGTGGCGTCGCCGCCTTACTCCGAAAGGCTTTTCCGACGTGGTCACCTGCTGCAATCAAATCTGCTCTGATCACCACTTCTTATTCTTTGGACAGTTCCGGCAAACCAATAAAAGATCTCTCCACAAGCGAAGAATCAAATCCATTCGTCCATGGCGCCGGCCATATCAATCCCAACCAAGCTCTGAATCCAGGCTTGATTTATGATCTCACTCCTCAAGATTACGTTTCATTTCTCTGCTCAATTGGTTACGATTCTAAACAAATAGCTGTTTTCGTGAAGGGTTCTTCATACTTCCAACTTTGTGAACACAAGCTGACCAACCCCGGCAACCTGAATTACCCATCTTTCTCTGTCGTGTTCGACGAGGAGGAGGTGGTGAAATACACAAGAACAGTGACAAATGTAGGAGATGAAACTGAAGTTGTTTATGAAGTGAAAGTAGAAGCTCCACAAGGCGTTGTAATCAGTGTTGTTCCAAACAAGCTGGAGTTCAATAAAGAGAAGACAACACAGTCTTATGAGATCACATTCACCAAAATCAATGGATTCAAGGAATCAGCAAGCTTTGGATCAATTCAATGGGGTGATGGAATCCACAGTGTTAGGAGTCCAATCGCTGTGTCCTTTAAGACTGGAGGATCCATAGCTTCGATGTAA

Protein sequence

MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKEKTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM*
Homology
BLAST of CsGy7G020730 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 8.6e-286
Identity = 496/773 (64.17%), Postives = 597/773 (77.23%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA   L S  F F  L CFF    S S +  E+YIVHV +S KPSLFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFS-PSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           L SSP P  LLY+Y RA +GFSAR++ +Q   LRR P +ISVIPDQ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
            + N GLW+++NY +DVI+GVLDTGIWPE PSFSD GL P+P+ WKG C+ G    A +C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLK-VSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYA 240
           NRK+IGARA++ GY +   G+ K  + + +S RDTEGHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 300
           RG A GMAS+ARIAAYKICW  GCYDSDILAAMDQA++DGV VISLSVG+SG AP Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGA 420
           F+G SLY+G+ L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KG A
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGT 480
           VK AGG GM+LANT E+GEEL ADSHL+P TMVGA AG+++RDYI T  +PTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L+IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG+PI+DL+T + SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCE-HKL 660
            F+HGAGH++PN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++  + +  CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 TNPGNLNYPSFSVVF-DEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEF 720
              G+LNYPSFSVVF    EVVKY R V NVG   + VYEV V++P  V I V P+KL F
Sbjct: 661 RTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 720

Query: 721 NKEKTTQSYEITFTKI-----NGFKESASFGSIQWGDGIHSVRSPIAVSFKTG 766
           +KEK+   YE+TF  +      G      FGSI+W DG H V+SP+AV +  G
Sbjct: 721 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQG 772

BLAST of CsGy7G020730 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 4.8e-220
Identity = 411/762 (53.94%), Postives = 524/762 (68.77%), Query Frame = 0

Query: 9   SSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPT 68
           SS  FF L C      S S  +Q TYIVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 69  KLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLG-L 128
           +LLY YE A +GFS R+T  +A+ L   PG+ISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 129 WADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGA 188
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+ WKG C+ G   +A  CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 189 RAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMA 248
           R +  GYES + G +  S + +S RD +GHGTHT+STAAGS V  AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 249 SRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFG 308
            RAR+A YK+CW  GC+ SDILAA+D+AI+D V+V+S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYS 368
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 369 GDPLGDSKLQLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSA 428
           G+ L D  L  +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 429 GGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGD 488
           GG+GM+LANT  NGEEL+AD+HL+P T VG  AG+ +R Y+ TDPNPTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 489 SPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIIS 548
             P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PTGL  D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 549 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVH 608
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     GKP+ D++T + S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 609 GAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGN 668
           GAGH++P  A NPGLIYDLT +DY+ FLC++ Y S QI    + +  +     K  +  +
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN--YTCDPSKSYSVAD 666

Query: 669 LNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEA-PQGVVISVVPNKLEFNKEKT 728
           LNYPSF+V  D     KYTRTVT+VG      Y VKV +   GV ISV P  L F +   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGAG--TYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 729 TQSYEITFTKINGFKESA--SFGSIQWGDGIHSVRSPIAVSF 763
            +SY +TFT ++  K S   SFGSI+W DG H V SP+A+S+
Sbjct: 727 KKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of CsGy7G020730 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 8.7e-214
Identity = 400/767 (52.15%), Postives = 514/767 (67.01%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+    SSS      F F  L        ++TYI+ V+ S+KP  F +HH W++S L S
Sbjct: 1   MASSSSSSSSITIITTFLFLLL----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEE-LRRVPGIISVIPDQIRQLHTTRTPHFL 120
            SS      LLY Y  + +GFSA + + +A+  L     I+ +  D +  LHTTRTP FL
Sbjct: 61  ESS------LLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFL 120

Query: 121 GLADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFA 180
           GL    G+    + ++ VIIGVLDTG+WPE  SF D  +  +P++WKG C++G    +  
Sbjct: 121 GLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKL 180

Query: 181 CNRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYA 240
           CN+K+IGAR++  G++    G      +  S RD +GHGTHT++TAAGS V NASF  YA
Sbjct: 181 CNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 300
            G ARGMA+RAR+A YK+CW  GC+ SDILAAMD+AI DGVDV+SLS+G  G AP YYRD
Sbjct: 241 AGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP-YYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           +IAIGAF AM+ GV VSCSAGNSGP   +  N+APW++TVGA T+DR+F A   LG+G+ 
Sbjct: 301 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 360

Query: 361 FSGVSLYSGDPLGDSKLQLVY--GGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKG 420
            +GVSLYSG  +G   L+LVY  G    S  C  GSLDSS V GKIVVCDRG NARV KG
Sbjct: 361 LTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKG 420

Query: 421 GAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFR 480
             V+ AGGLGM++ANT  +GEEL+ADSHL+P   VG   G+ LR+Y+ +D  PTA +VF+
Sbjct: 421 AVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFK 480

Query: 481 GTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRV 540
           GTV+ D  P+P VAAFSSRGPN  T EILKPDVI PGVNILAGWS    PTGL+ D RR 
Sbjct: 481 GTVL-DVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 540

Query: 541 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEE 600
           +FNI+SGTSMSCPH+SG+A LL+ A P WSP+AIKSAL+TT+Y LD++  P+ D + +  
Sbjct: 541 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 600

Query: 601 SNPFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHK 660
           SNP+ HG+GH++P +AL+PGL+YD++ ++Y+ FLCS+ Y    I   VK  S    C  K
Sbjct: 601 SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSV--NCSKK 660

Query: 661 LTNPGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEF 720
            ++PG LNYPSFSV+F  + VV+YTR VTNVG  +  VY+V V     V ISV P+KL F
Sbjct: 661 FSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASS-VYKVTVNGAPSVGISVKPSKLSF 720

Query: 721 NKEKTTQSYEITFTKING--FKESASFGSIQWGDGIHSVRSPIAVSF 763
                 + Y +TF    G      A FGSI W +  H VRSP+A S+
Sbjct: 721 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of CsGy7G020730 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.0e-209
Identity = 392/778 (50.39%), Postives = 521/778 (66.97%), Query Frame = 0

Query: 11  FFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKL 70
           F+FFFL        S S  N  TYIVHV    KPS+F +H HW++S L SL+SS  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSAR+T+  A +L   P +ISVIP+Q+R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 ADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP +WKG C   +     ACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMAS 250
            +  GYE+   G +  +++F+S RD++GHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 RARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGA 310
           +AR+AAYK+CW  GCYDSDILAA D A++DGVDVISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 370
           +  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-QLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVK 430
             L   ++  LVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 SAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYI------HTDPNPTATIV 490
             GGLGM++AN   +GE L+AD H++P T VGA  G+++R YI       +  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPR 550
           F+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+G+  D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG+P+ D ST 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 EESNPFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCE 670
             S+   +G+GH++P +A++PGL+YD+T  DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLTNPGNLNYPSFSVVFDEEEVVK----YTRTVTNVGDETEVVYEVKVEAPQGVVIS 730
              +  + GNLNYPSFSVVF +    K    + RTVTNVGD ++ VYE+K+  P+G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VVPNKLEFNKEKTTQSYEI----TFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFK 764
           V P KL F +     S+ +    T  K++    +   G I W DG  +V SP+ V+ +
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of CsGy7G020730 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 2.2e-201
Identity = 375/768 (48.83%), Postives = 504/768 (65.62%), Query Frame = 0

Query: 8   SSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHP 67
           +SS     LF  FP I   + +  +T+I  +     PS+F +H+HW+S      +     
Sbjct: 2   ASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS------TEFAEE 61

Query: 68  TKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGL 127
           +++++ Y    +GFSA +T  +A+ LR  P +++V  D+ R+LHTTR+P FLGL +  GL
Sbjct: 62  SRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGL 121

Query: 128 WADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGA 187
           W++++Y  DVIIGV DTGIWPER SFSD  L P+P RW+G C++G   S   CNRKIIGA
Sbjct: 122 WSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 181

Query: 188 RAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMA 247
           R +  G ++ + G +  + +F S RD +GHGTHT+STAAG     AS   YA G A+G+A
Sbjct: 182 RFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVA 241

Query: 248 SRARIAAYKICW-EFGCYDSDILAAMDQAISDGVDVISLSV-GSSGRAPAYYRDSIAIGA 307
            +ARIAAYK+CW + GC DSDILAA D A+ DGVDVIS+S+ G  G    YY D IAIG+
Sbjct: 242 PKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGS 301

Query: 308 FGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           +GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVSL
Sbjct: 302 YGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSL 361

Query: 368 YSGDPLGDSKLQLVYGGDCG---SRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKS 427
           Y+G PL      +VY G  G   +  C   +LD  +V GKIV+CDRG + RVAKG  VK 
Sbjct: 362 YAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKK 421

Query: 428 AGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIG 487
           AGG+GM+LAN   NGE L+ D+HLIP   VG+  G++++ Y  + PNP A+I FRGT++G
Sbjct: 422 AGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVG 481

Query: 488 DSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNII 547
              PAP +A+FS RGPN  + EILKPD+IAPGVNILA W+    PTGL  DPR+ EFNI+
Sbjct: 482 -IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 541

Query: 548 SGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFV 607
           SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  +D+S + + D ST + + P+ 
Sbjct: 542 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 601

Query: 608 HGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPG 667
           +G+GH+N  +A+NPGL+YD+T  DY++FLCSIGY  K I V  +  +  +    +  +PG
Sbjct: 602 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSPG 661

Query: 668 NLNYPSFSVVF--DEEEVVKYT--RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFN 727
           NLNYPS + VF  +   +V  T  RT TNVG + E VY  ++E+P+GV ++V P +L F 
Sbjct: 662 NLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVFT 721

Query: 728 KEKTTQSYEITFT----KINGFKESASFGSIQWGD-GIHSVRSPIAVS 762
                +SY +T T     +   +  A FGS+ W D G H VRSPI V+
Sbjct: 722 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of CsGy7G020730 vs. NCBI nr
Match: XP_004141706.1 (subtilisin-like protease SBT1.4 [Cucumis sativus] >KGN45480.1 hypothetical protein Csa_016246 [Cucumis sativus])

HSP 1 Score: 1532 bits (3967), Expect = 0.0
Identity = 771/771 (100.00%), Postives = 771/771 (100.00%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720
           PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of CsGy7G020730 vs. NCBI nr
Match: XP_008462294.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 [Cucumis melo])

HSP 1 Score: 1455 bits (3766), Expect = 0.0
Identity = 733/771 (95.07%), Postives = 749/771 (97.15%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFF+   FPL+FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYERAANGFSARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARA+F GYESNLRGSLK SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKL LVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHI+PNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+N
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720
           PGNLNYPSFSVVFD+ EVVKYTRTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           KTT SYEITFTKINGFKESASFGSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTG-SIASM 770

BLAST of CsGy7G020730 vs. NCBI nr
Match: TYK03935.1 (subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1425 bits (3689), Expect = 0.0
Identity = 715/749 (95.46%), Postives = 731/749 (97.60%), Query Frame = 0

Query: 23  IFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKLLYNYERAANGFS 82
           +FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILESLS SPHPT+LLYNYERAANGFS
Sbjct: 23  LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 82

Query: 83  ARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142

Query: 143 DTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGARAYFYGYESNLRGSL 202
           DTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA ACNRKIIGARA+F+GYESNLRGSL
Sbjct: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202

Query: 203 KVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMASRARIAAYKICWEFG 262
           K SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHG+VVSCSAGNS
Sbjct: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322

Query: 323 GPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLQLVYGG 382
           GPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVFSGVSLYSGDPLGDSKL LVYGG
Sbjct: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382

Query: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442

Query: 443 DSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRT 502
           DSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAP+VA+FSSRGPNYRT
Sbjct: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562

Query: 563 FPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVHGAGHINPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNPFVHGAGHI+PNQALNPGLIYDL
Sbjct: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGNLNYPSFSVVFDEEEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+NPGNLNYPSFSVVFD+ EVVKYT
Sbjct: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682

Query: 683 RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKEKTTQSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKEKTT SYEITFTKINGFKESASF
Sbjct: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742

Query: 743 GSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           GSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 743 GSIQWSDGIHNVRSPIAVSFKTG-SIASM 770

BLAST of CsGy7G020730 vs. NCBI nr
Match: KAA0059392.1 (subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1425 bits (3689), Expect = 0.0
Identity = 715/749 (95.46%), Postives = 731/749 (97.60%), Query Frame = 0

Query: 23  IFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKLLYNYERAANGFS 82
           +FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILESLS SPHPT+LLYNYERAANGFS
Sbjct: 3   LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 62

Query: 83  ARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 63  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 122

Query: 143 DTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGARAYFYGYESNLRGSL 202
           DTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA ACNRKIIGARA+F+GYESNLRGSL
Sbjct: 123 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 182

Query: 203 KVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMASRARIAAYKICWEFG 262
           K SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 183 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 242

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHG+VVSCSAGNS
Sbjct: 243 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 302

Query: 323 GPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLQLVYGG 382
           GPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVFSGVSLYSGDPLGDSKL LVYGG
Sbjct: 303 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 362

Query: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 363 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 422

Query: 443 DSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRT 502
           DSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAP+VA+FSSRGPNYRT
Sbjct: 423 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 482

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 483 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 542

Query: 563 FPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVHGAGHINPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNPFVHGAGHI+PNQALNPGLIYDL
Sbjct: 543 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 602

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGNLNYPSFSVVFDEEEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+NPGNLNYPSFSVVFD+ EVVKYT
Sbjct: 603 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 662

Query: 683 RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKEKTTQSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKEKTT SYEITFTKINGFKESASF
Sbjct: 663 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 722

Query: 743 GSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           GSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 723 GSIQWSDGIHNVRSPIAVSFKTG-SIASM 750

BLAST of CsGy7G020730 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1380 bits (3573), Expect = 0.0
Identity = 697/771 (90.40%), Postives = 724/771 (93.90%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA  HL SS FF F L CF P+IFSRS ENQET+IVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MAKSHLCSSLFFLFIL-CFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYERA NGFSAR+T  QA +LR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LADNLGLW DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA WKGTC+TGEG SA  C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARAYFYGY SN   S   SSDFKSARDTEGHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSK+ LVYGGDCGSRYCYSGSL+ SKVAGKIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLRDYIH+DPNPTA IVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILA WSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHI+PN+ALNPGLIYD++PQDYVSFLCSIGYDSKQIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720
           PGNLNYPSF+VVF+ E+VVKYTRTVTNVGDE++VVYEVKVEAPQGV ISVVPNKL FN E
Sbjct: 661 PGNLNYPSFAVVFNGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNAE 720

Query: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           KTT SYEITFTKI+GF E+ASFGSIQW DG H+VRSPIAVSF TG SIASM
Sbjct: 721 KTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTG-SIASM 769

BLAST of CsGy7G020730 vs. ExPASy TrEMBL
Match: A0A0A0K6Z1 (Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1)

HSP 1 Score: 1532 bits (3967), Expect = 0.0
Identity = 771/771 (100.00%), Postives = 771/771 (100.00%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720
           PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of CsGy7G020730 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1455 bits (3766), Expect = 0.0
Identity = 733/771 (95.07%), Postives = 749/771 (97.15%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MANFHLPSSSFFFFF+   FPL+FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYERAANGFSARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARA+F GYESNLRGSLK SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKL LVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHI+PNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+N
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720
           PGNLNYPSFSVVFD+ EVVKYTRTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKE
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           KTT SYEITFTKINGFKESASFGSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTG-SIASM 770

BLAST of CsGy7G020730 vs. ExPASy TrEMBL
Match: A0A5A7UU12 (Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001180 PE=3 SV=1)

HSP 1 Score: 1425 bits (3689), Expect = 0.0
Identity = 715/749 (95.46%), Postives = 731/749 (97.60%), Query Frame = 0

Query: 23  IFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKLLYNYERAANGFS 82
           +FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILESLS SPHPT+LLYNYERAANGFS
Sbjct: 3   LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 62

Query: 83  ARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 63  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 122

Query: 143 DTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGARAYFYGYESNLRGSL 202
           DTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA ACNRKIIGARA+F+GYESNLRGSL
Sbjct: 123 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 182

Query: 203 KVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMASRARIAAYKICWEFG 262
           K SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 183 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 242

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHG+VVSCSAGNS
Sbjct: 243 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 302

Query: 323 GPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLQLVYGG 382
           GPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVFSGVSLYSGDPLGDSKL LVYGG
Sbjct: 303 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 362

Query: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 363 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 422

Query: 443 DSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRT 502
           DSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAP+VA+FSSRGPNYRT
Sbjct: 423 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 482

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 483 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 542

Query: 563 FPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVHGAGHINPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNPFVHGAGHI+PNQALNPGLIYDL
Sbjct: 543 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 602

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGNLNYPSFSVVFDEEEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+NPGNLNYPSFSVVFD+ EVVKYT
Sbjct: 603 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 662

Query: 683 RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKEKTTQSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKEKTT SYEITFTKINGFKESASF
Sbjct: 663 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 722

Query: 743 GSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           GSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 723 GSIQWSDGIHNVRSPIAVSFKTG-SIASM 750

BLAST of CsGy7G020730 vs. ExPASy TrEMBL
Match: A0A5D3BXR8 (Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001320 PE=3 SV=1)

HSP 1 Score: 1425 bits (3689), Expect = 0.0
Identity = 715/749 (95.46%), Postives = 731/749 (97.60%), Query Frame = 0

Query: 23  IFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKLLYNYERAANGFS 82
           +FSRSLE QETYIVHVSKSEKPSLFSSHHHWHSSILESLS SPHPT+LLYNYERAANGFS
Sbjct: 23  LFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTRLLYNYERAANGFS 82

Query: 83  ARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142
           ARITT QAEELRRVPGIISVIPD+IRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL
Sbjct: 83  ARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYADDVIIGVL 142

Query: 143 DTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGARAYFYGYESNLRGSL 202
           DTGIWPERPSFSDEGL+PVPARWKGTCDTGEG SA ACNRKIIGARA+F+GYESNLRGSL
Sbjct: 143 DTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALACNRKIIGARAFFFGYESNLRGSL 202

Query: 203 KVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMASRARIAAYKICWEFG 262
           K SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYARGEARGMASRARIAAYKICWEFG
Sbjct: 203 KGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFG 262

Query: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNS 322
           CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHG+VVSCSAGNS
Sbjct: 263 CYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGAMQHGIVVSCSAGNS 322

Query: 323 GPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLQLVYGG 382
           GPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVFSGVSLYSGDPLGDSKL LVYGG
Sbjct: 323 GPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVFSGVSLYSGDPLGDSKLPLVYGG 382

Query: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442
           DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA
Sbjct: 383 DCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLA 442

Query: 443 DSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRT 502
           DSHLIPGTMVG IAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAP+VA+FSSRGPNYRT
Sbjct: 443 DSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTVIGDSPPAPKVASFSSRGPNYRT 502

Query: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562
           AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA
Sbjct: 503 AEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIISGTSMSCPHVSGVAALLRKA 562

Query: 563 FPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVHGAGHINPNQALNPGLIYDL 622
           FPTWSPAAIKSALITTSYSLDSSG PIKDL+TSEESNPFVHGAGHI+PNQALNPGLIYDL
Sbjct: 563 FPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNPFVHGAGHIDPNQALNPGLIYDL 622

Query: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGNLNYPSFSVVFDEEEVVKYT 682
           TPQDYVSFLCSIGYDSKQIAVFVKGSSY QLCEHKL+NPGNLNYPSFSVVFD+ EVVKYT
Sbjct: 623 TPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSNPGNLNYPSFSVVFDDGEVVKYT 682

Query: 683 RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKEKTTQSYEITFTKINGFKESASF 742
           RTVTNVGDETEVVY VKVEAPQGV ISVVPNKLEFNKEKTT SYEITFTKINGFKESASF
Sbjct: 683 RTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKEKTTLSYEITFTKINGFKESASF 742

Query: 743 GSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
           GSIQW DGIH+VRSPIAVSFKTG SIASM
Sbjct: 743 GSIQWSDGIHNVRSPIAVSFKTG-SIASM 770

BLAST of CsGy7G020730 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1344 bits (3478), Expect = 0.0
Identity = 675/773 (87.32%), Postives = 718/773 (92.88%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MAN HL SS FFF  L CF P+IFSRSL+NQET+IVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLHLCSSGFFFLIL-CFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           LS SPHPTKLLY+YE AANGFSARIT  QA ELRR+PG+ISVIPD++RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
           LAD+ GLWA+TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA WKGTC TG G SA AC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
           NRKIIGARAYF GYES +R SL+ SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAI+DGV +ISLSVGSSGR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSGDPLGDSKL LVY GDCG RYCYSG+LDSSKVAGKIVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
           K+AGGLGM+LANTEENGEELLADSHLIPGTM+G IAGN L+DYIH+DP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG+PI DLSTS++SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
           FVHGAGHI+PN+ALNPGLIYDL PQDYVSFLCSIGYDS+QIAVFVK S Y QLCEHKLT+
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFSVVFDEE--EVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFN 720
           PGNLNYPSF+VVFD +  EVVKYTRTVTNVGDE + VYEVKVE+PQGV I+V+PNKLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 KEKTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
            +  TQSYEITFTKING K SASFGSIQW DG H VRSP+AVSF +G  IASM
Sbjct: 721 AKDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSG-YIASM 771

BLAST of CsGy7G020730 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 984.2 bits (2543), Expect = 6.1e-287
Identity = 496/773 (64.17%), Postives = 597/773 (77.23%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA   L S  F F  L CFF    S S +  E+YIVHV +S KPSLFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFS-PSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
           L SSP P  LLY+Y RA +GFSAR++ +Q   LRR P +ISVIPDQ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
            + N GLW+++NY +DVI+GVLDTGIWPE PSFSD GL P+P+ WKG C+ G    A +C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFYGYESNLRGSLK-VSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYA 240
           NRK+IGARA++ GY +   G+ K  + + +S RDTEGHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 300
           RG A GMAS+ARIAAYKICW  GCYDSDILAAMDQA++DGV VISLSVG+SG AP Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGA 420
           F+G SLY+G+ L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KG A
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGT 480
           VK AGG GM+LANT E+GEEL ADSHL+P TMVGA AG+++RDYI T  +PTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L+IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG+PI+DL+T + SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCE-HKL 660
            F+HGAGH++PN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++  + +  CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 TNPGNLNYPSFSVVF-DEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEF 720
              G+LNYPSFSVVF    EVVKY R V NVG   + VYEV V++P  V I V P+KL F
Sbjct: 661 RTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 720

Query: 721 NKEKTTQSYEITFTKI-----NGFKESASFGSIQWGDGIHSVRSPIAVSFKTG 766
           +KEK+   YE+TF  +      G      FGSI+W DG H V+SP+AV +  G
Sbjct: 721 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQG 772

BLAST of CsGy7G020730 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 765.8 bits (1976), Expect = 3.4e-221
Identity = 411/762 (53.94%), Postives = 524/762 (68.77%), Query Frame = 0

Query: 9   SSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPT 68
           SS  FF L C      S S  +Q TYIVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 69  KLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLG-L 128
           +LLY YE A +GFS R+T  +A+ L   PG+ISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 129 WADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGA 188
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+ WKG C+ G   +A  CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 189 RAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMA 248
           R +  GYES + G +  S + +S RD +GHGTHT+STAAGS V  AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 249 SRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFG 308
            RAR+A YK+CW  GC+ SDILAA+D+AI+D V+V+S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYS 368
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 369 GDPLGDSKLQLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKSA 428
           G+ L D  L  +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 429 GGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIGD 488
           GG+GM+LANT  NGEEL+AD+HL+P T VG  AG+ +R Y+ TDPNPTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 489 SPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNIIS 548
             P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PTGL  D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 549 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFVH 608
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     GKP+ D++T + S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 609 GAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPGN 668
           GAGH++P  A NPGLIYDLT +DY+ FLC++ Y S QI    + +  +     K  +  +
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN--YTCDPSKSYSVAD 666

Query: 669 LNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEA-PQGVVISVVPNKLEFNKEKT 728
           LNYPSF+V  D     KYTRTVT+VG      Y VKV +   GV ISV P  L F +   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGAG--TYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 729 TQSYEITFTKINGFKESA--SFGSIQWGDGIHSVRSPIAVSF 763
            +SY +TFT ++  K S   SFGSI+W DG H V SP+A+S+
Sbjct: 727 KKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of CsGy7G020730 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 745.0 bits (1922), Expect = 6.2e-215
Identity = 400/767 (52.15%), Postives = 514/767 (67.01%), Query Frame = 0

Query: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+    SSS      F F  L        ++TYI+ V+ S+KP  F +HH W++S L S
Sbjct: 1   MASSSSSSSSITIITTFLFLLL----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS 60

Query: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEE-LRRVPGIISVIPDQIRQLHTTRTPHFL 120
            SS      LLY Y  + +GFSA + + +A+  L     I+ +  D +  LHTTRTP FL
Sbjct: 61  ESS------LLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFL 120

Query: 121 GLADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFA 180
           GL    G+    + ++ VIIGVLDTG+WPE  SF D  +  +P++WKG C++G    +  
Sbjct: 121 GLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKL 180

Query: 181 CNRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYA 240
           CN+K+IGAR++  G++    G      +  S RD +GHGTHT++TAAGS V NASF  YA
Sbjct: 181 CNKKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRD 300
            G ARGMA+RAR+A YK+CW  GC+ SDILAAMD+AI DGVDV+SLS+G  G AP YYRD
Sbjct: 241 AGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLG-GGSAP-YYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           +IAIGAF AM+ GV VSCSAGNSGP   +  N+APW++TVGA T+DR+F A   LG+G+ 
Sbjct: 301 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 360

Query: 361 FSGVSLYSGDPLGDSKLQLVY--GGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKG 420
            +GVSLYSG  +G   L+LVY  G    S  C  GSLDSS V GKIVVCDRG NARV KG
Sbjct: 361 LTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKG 420

Query: 421 GAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFR 480
             V+ AGGLGM++ANT  +GEEL+ADSHL+P   VG   G+ LR+Y+ +D  PTA +VF+
Sbjct: 421 AVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFK 480

Query: 481 GTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRV 540
           GTV+ D  P+P VAAFSSRGPN  T EILKPDVI PGVNILAGWS    PTGL+ D RR 
Sbjct: 481 GTVL-DVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 540

Query: 541 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEE 600
           +FNI+SGTSMSCPH+SG+A LL+ A P WSP+AIKSAL+TT+Y LD++  P+ D + +  
Sbjct: 541 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 600

Query: 601 SNPFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHK 660
           SNP+ HG+GH++P +AL+PGL+YD++ ++Y+ FLCS+ Y    I   VK  S    C  K
Sbjct: 601 SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSV--NCSKK 660

Query: 661 LTNPGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEF 720
            ++PG LNYPSFSV+F  + VV+YTR VTNVG  +  VY+V V     V ISV P+KL F
Sbjct: 661 FSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASS-VYKVTVNGAPSVGISVKPSKLSF 720

Query: 721 NKEKTTQSYEITFTKING--FKESASFGSIQWGDGIHSVRSPIAVSF 763
                 + Y +TF    G      A FGSI W +  H VRSP+A S+
Sbjct: 721 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of CsGy7G020730 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 731.5 bits (1887), Expect = 7.1e-211
Identity = 392/778 (50.39%), Postives = 521/778 (66.97%), Query Frame = 0

Query: 11  FFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHPTKL 70
           F+FFFL        S S  N  TYIVHV    KPS+F +H HW++S L SL+SS  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSAR+T+  A +L   P +ISVIP+Q+R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 ADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP +WKG C   +     ACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMAS 250
            +  GYE+   G +  +++F+S RD++GHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEAT-NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 RARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDSIAIGAFGA 310
           +AR+AAYK+CW  GCYDSDILAA D A++DGVDVISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 370
           +  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-QLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVK 430
             L   ++  LVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 SAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYI------HTDPNPTATIV 490
             GGLGM++AN   +GE L+AD H++P T VGA  G+++R YI       +  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPR 550
           F+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+G+  D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG+P+ D ST 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 EESNPFVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCE 670
             S+   +G+GH++P +A++PGL+YD+T  DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLTNPGNLNYPSFSVVFDEEEVVK----YTRTVTNVGDETEVVYEVKVEAPQGVVIS 730
              +  + GNLNYPSFSVVF +    K    + RTVTNVGD ++ VYE+K+  P+G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VVPNKLEFNKEKTTQSYEI----TFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFK 764
           V P KL F +     S+ +    T  K++    +   G I W DG  +V SP+ V+ +
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of CsGy7G020730 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 703.7 bits (1815), Expect = 1.6e-202
Identity = 375/768 (48.83%), Postives = 504/768 (65.62%), Query Frame = 0

Query: 8   SSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILESLSSSPHP 67
           +SS     LF  FP I   + +  +T+I  +     PS+F +H+HW+S      +     
Sbjct: 2   ASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYS------TEFAEE 61

Query: 68  TKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLGLADNLGL 127
           +++++ Y    +GFSA +T  +A+ LR  P +++V  D+ R+LHTTR+P FLGL +  GL
Sbjct: 62  SRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGL 121

Query: 128 WADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFACNRKIIGA 187
           W++++Y  DVIIGV DTGIWPER SFSD  L P+P RW+G C++G   S   CNRKIIGA
Sbjct: 122 WSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGA 181

Query: 188 RAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYARGEARGMA 247
           R +  G ++ + G +  + +F S RD +GHGTHT+STAAG     AS   YA G A+G+A
Sbjct: 182 RFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVA 241

Query: 248 SRARIAAYKICW-EFGCYDSDILAAMDQAISDGVDVISLSV-GSSGRAPAYYRDSIAIGA 307
            +ARIAAYK+CW + GC DSDILAA D A+ DGVDVIS+S+ G  G    YY D IAIG+
Sbjct: 242 PKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGS 301

Query: 308 FGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           +GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVSL
Sbjct: 302 YGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSL 361

Query: 368 YSGDPLGDSKLQLVYGGDCG---SRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAVKS 427
           Y+G PL      +VY G  G   +  C   +LD  +V GKIV+CDRG + RVAKG  VK 
Sbjct: 362 YAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKK 421

Query: 428 AGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTVIG 487
           AGG+GM+LAN   NGE L+ D+HLIP   VG+  G++++ Y  + PNP A+I FRGT++G
Sbjct: 422 AGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVG 481

Query: 488 DSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFNII 547
              PAP +A+FS RGPN  + EILKPD+IAPGVNILA W+    PTGL  DPR+ EFNI+
Sbjct: 482 -IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNIL 541

Query: 548 SGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNPFV 607
           SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  +D+S + + D ST + + P+ 
Sbjct: 542 SGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYD 601

Query: 608 HGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTNPG 667
           +G+GH+N  +A+NPGL+YD+T  DY++FLCSIGY  K I V  +  +  +    +  +PG
Sbjct: 602 YGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSPG 661

Query: 668 NLNYPSFSVVF--DEEEVVKYT--RTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFN 727
           NLNYPS + VF  +   +V  T  RT TNVG + E VY  ++E+P+GV ++V P +L F 
Sbjct: 662 NLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVFT 721

Query: 728 KEKTTQSYEITFT----KINGFKESASFGSIQWGD-GIHSVRSPIAVS 762
                +SY +T T     +   +  A FGS+ W D G H VRSPI V+
Sbjct: 722 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVJ18.6e-28664.17Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
O653514.8e-22053.94Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF68.7e-21452.15Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9LUM31.0e-20950.39Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O496072.2e-20148.83Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_004141706.10.0100.00subtilisin-like protease SBT1.4 [Cucumis sativus] >KGN45480.1 hypothetical prote... [more]
XP_008462294.10.095.07PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 [Cucumis melo][more]
TYK03935.10.095.46subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa][more]
KAA0059392.10.095.46subtilisin-like protease SBT1.4 [Cucumis melo var. makuwa][more]
XP_038897980.10.090.40subtilisin-like protease SBT1.4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0K6Z10.0100.00Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1[more]
A0A1S3CGN80.095.07LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=... [more]
A0A5A7UU120.095.46Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5D3BXR80.095.46Subtilisin-like protease SBT1.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1HMN70.087.32subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE... [more]
Match NameE-valueIdentityDescription
AT3G14067.16.1e-28764.17Subtilase family protein [more]
AT5G67360.13.4e-22153.94Subtilase family protein [more]
AT2G05920.16.2e-21552.15Subtilase family protein [more]
AT3G14240.17.1e-21150.39Subtilase family protein [more]
AT4G34980.11.6e-20248.83subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 134..153
score: 31.98
coord: 543..559
score: 59.45
coord: 213..226
score: 49.57
IPR003137PA domainPFAMPF02225PAcoord: 377..460
e-value: 6.0E-9
score: 35.9
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 135..585
e-value: 1.3E-50
score: 172.7
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 136..627
e-value: 2.5E-183
score: 612.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 19..705
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 24..111
e-value: 4.1E-22
score: 80.4
NoneNo IPR availableGENE3D3.50.30.30coord: 345..481
e-value: 2.5E-183
score: 612.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 631..763
e-value: 4.7E-38
score: 131.8
NoneNo IPR availablePANTHERPTHR10795:SF680SUBTILISIN-LIKE PROTEASE SBT1.6coord: 21..764
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 109..614
score: 28.019293
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 351..474
e-value: 1.7224E-39
score: 139.856
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 663..760
e-value: 6.5E-30
score: 103.3
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 33..111
e-value: 6.7E-18
score: 65.1
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 21..764
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 544..554
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 109..580
e-value: 1.69753E-145
score: 427.014

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy7G020730.1CsGy7G020730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity