Tan0021658.1 (mRNA) Snake gourd v1

Overview


Name	Tan0021658.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	LG01: 22213237 .. 22213812 (+)
Sequence length	576
RNA-Seq Expression	Tan0021658.1
Synteny	Tan0021658.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCTTTCATGTTCCCCTTCCAATCACAACCATTCTCCTCATGTTCTTCCTCATCAACCAATCACATATTGCAAAAACTCAACCAATAAATGACACTCAATTCATCAATACCACTTGTCAATCCACACCCTACCCTCAACTCTGCCTCTCCTCCCTCTCCGTCGACGCCGCCGCCATCCACGGTAGCTACCGCCTGATAACCGTCGCCGCTCTCTCCATCGCCCTCACACACACGCGCTCCGCTTCATCGGCGGTCGCGAGCCTGGCCGAGTCGAATGCCTTAGCACCGCGAGAGAGCTATGCTCTGAATGATTGCATTGACGAATTTGCAGACTCTATTGAAGAGTTAAAAATGGCTGTGGCGGAGTTGAAGTATGATGACAATGCTCGGGTCGAAACCGAGGATATTCGAACGTGGGTGAGCGCTGCTCTGACCGACGATGACACGTGCATGGATGGGTTCGCCGGAGACGCCATGAATGGCCGTGTGAAGGAGTCGATGAGAGCATTGGTGGTGGATGTTGCTCAAATGACAAGCATTGCTCTCTCGCTTGTTAGCCAGTTAAAGTGA

mRNA sequence

ATGGAATCCTTTCATGTTCCCCTTCCAATCACAACCATTCTCCTCATGTTCTTCCTCATCAACCAATCACATATTGCAAAAACTCAACCAATAAATGACACTCAATTCATCAATACCACTTGTCAATCCACACCCTACCCTCAACTCTGCCTCTCCTCCCTCTCCGTCGACGCCGCCGCCATCCACGGTAGCTACCGCCTGATAACCGTCGCCGCTCTCTCCATCGCCCTCACACACACGCGCTCCGCTTCATCGGCGGTCGCGAGCCTGGCCGAGTCGAATGCCTTAGCACCGCGAGAGAGCTATGCTCTGAATGATTGCATTGACGAATTTGCAGACTCTATTGAAGAGTTAAAAATGGCTGTGGCGGAGTTGAAGTATGATGACAATGCTCGGGTCGAAACCGAGGATATTCGAACGTGGGTGAGCGCTGCTCTGACCGACGATGACACGTGCATGGATGGGTTCGCCGGAGACGCCATGAATGGCCGTGTGAAGGAGTCGATGAGAGCATTGGTGGTGGATGTTGCTCAAATGACAAGCATTGCTCTCTCGCTTGTTAGCCAGTTAAAGTGA

Coding sequence (CDS)

ATGGAATCCTTTCATGTTCCCCTTCCAATCACAACCATTCTCCTCATGTTCTTCCTCATCAACCAATCACATATTGCAAAAACTCAACCAATAAATGACACTCAATTCATCAATACCACTTGTCAATCCACACCCTACCCTCAACTCTGCCTCTCCTCCCTCTCCGTCGACGCCGCCGCCATCCACGGTAGCTACCGCCTGATAACCGTCGCCGCTCTCTCCATCGCCCTCACACACACGCGCTCCGCTTCATCGGCGGTCGCGAGCCTGGCCGAGTCGAATGCCTTAGCACCGCGAGAGAGCTATGCTCTGAATGATTGCATTGACGAATTTGCAGACTCTATTGAAGAGTTAAAAATGGCTGTGGCGGAGTTGAAGTATGATGACAATGCTCGGGTCGAAACCGAGGATATTCGAACGTGGGTGAGCGCTGCTCTGACCGACGATGACACGTGCATGGATGGGTTCGCCGGAGACGCCATGAATGGCCGTGTGAAGGAGTCGATGAGAGCATTGGTGGTGGATGTTGCTCAAATGACAAGCATTGCTCTCTCGCTTGTTAGCCAGTTAAAGTGA

Protein sequence

MESFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVSQLK

Homology

[Close]

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.4e-24
Identity = 69/168 (41.07%), Postives = 103/168 (61.31%), Query Frame = 0


Query: 26  AKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAALSIALTHTRSASS 85
           A   P N  QFI T+C  T YP +C  SLS  A  I  + + +   AL ++LT T+ A +
Sbjct: 20  ASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQT 79

Query: 86  AVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKYDDNAR-----VETEDIRT 145
            +  L +   L  R+  A++DC++E  DS++ +  +  E+K   +A+         ++ T
Sbjct: 80  FMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVET 139

Query: 146 WVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVS 189
           WVSAALTD+ TCMDGFAG  M+G++KES+RA VV VA++TS AL+LV+
Sbjct: 140 WVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.7e-22
Identity = 77/189 (40.74%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  ITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAI-HGSYRLI 69
           I  ++L+   I QS  A        QFI ++CQ+T YP LC+ +LS  A  I H + + +
Sbjct: 7   IFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDL 66

Query: 70  TVAALSIALTHTRSASSAVASLA-ESNALAPRESYALNDCIDEFADSIEELKMAVAELKY 129
              AL I+L   +S +  VA L  E+     RE  A+ DCI+   +S++ L  +V EL  
Sbjct: 67  AQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELAR 126

Query: 130 DDNARVETED-------IRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMT 189
             +A V +ED       ++TWVSAALTD+ TC+DGF+  AM G+VK  +R  VV VAQ+T
Sbjct: 127 AGHA-VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 186

[Close]

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.5e-21
Identity = 62/192 (32.29%), Postives = 107/192 (55.73%), Query Frame = 0


Query: 3   SFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIH 62
           +F + L +  + L    I  +   + +   DT+FI  +C++T YP  C  SLS  A+ I 
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 63  GSYRLITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAV 122
              R +   AL++++   +SA + V+ + +   +  R+  A+ DC++E  D+++ L  ++
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 123 AELKYDDNARVETE------DIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDV 182
            ELK+ +      +      ++RTW SAALTD+  CMDGF G AM G +K  +R  +V V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 183 AQMTSIALSLVS 189
           A+ TS AL+L++
Sbjct: 184 AEETSNALALIN 195

[Close]

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.6e-20
Identity = 63/158 (39.87%), Postives = 99/158 (62.66%), Query Frame = 0


Query: 36  FINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAALSIALTHTRSASSAVASLAESNA 95
           +I T+C  T Y  +C +SLS  A+ I  + + + V AL++ L+  +SAS  V +++    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 96  LAPRESYALNDCIDEFADSIEELKMAVAEL---KYDDNARVE--TEDIRTWVSAALTDDD 155
           L   E  A+ DC++E  DS+  L+ ++ EL    Y D+A+ E    D+ TWVSAALT+DD
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 156 TCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVS 189
           TCMDGF+   +   VK+ +R  VV+VA++TS AL+L++
Sbjct: 267 TCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 66/184 (35.87%), Postives = 107/184 (58.15%), Query Frame = 0


Query: 11  TTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITV 70
           T IL       ++ I   + IN   FI  +C++T YP +C++SL+  A +I  S R +  
Sbjct: 16  TAILTASSSAPRAAITSKRAIN---FIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAE 75

Query: 71  AALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKYDDN 130
            AL++ +T  +S    V  L    +L  RE  A+ DCI+E  D+++ L M++ E+K   +
Sbjct: 76  TALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGS 135

Query: 131 AR------VETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIAL 189
           A+          + +TW SAALT+ +TC DGFAG  M+GRVK S+RA ++++ + TS AL
Sbjct: 136 AKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNAL 195

[Close]

BLAST of Tan0021658.1 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-67
Identity = 142/191 (74.35%), Postives = 161/191 (84.29%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAA 60
           MES ++PLPI  ILL+  LINQS+IAK+QPINDTQFI TTCQSTPYP LCLSSLS DAA 
Sbjct: 1   MESSYLPLPIKAILLI-ILINQSNIAKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAAT 60

Query: 61  IHGSYRLITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKM 120
           IHGSYRL+T+AAL++ALT TRSASS + SLA+SN L PRE Y ++DCI+EF DSIEELKM
Sbjct: 61  IHGSYRLMTIAALTVALTRTRSASSVIQSLAKSNVLTPREGYVVSDCIEEFGDSIEELKM 120

Query: 121 AVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMT 180
           A+ ELK +D  R E EDIRTWVSAALTDDDTCMDGF GD MN  VKESM+ LVVD+ Q+T
Sbjct: 121 AIVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQLT 180

Query: 181 SIALSLVSQLK 192
           SIALSLVS LK
Sbjct: 181 SIALSLVSLLK 190

[Close]

BLAST of Tan0021658.1 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 264.6 bits (675), Expect = 6.5e-67
Identity = 140/192 (72.92%), Postives = 162/192 (84.38%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAA 60
           M+S + PLP+  ILL+  LINQS+IA +QP NDTQFI TTCQSTPYP LCLSSLS  AAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHGSYRLITVAALSIALTHTRSASSAVASLAE-SNALAPRESYALNDCIDEFADSIEELK 120
           IH SYRL+T+AAL++ALTHTRS SSAV SLA+ SNAL PRE Y + DCI+EF DS+EELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQM 180
           MA+ ELK ++  R ETEDIRTWVSAALTDDDTCMDG  GDAMNG +KES++A+VVDVAQ+
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSQLK 192
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of Tan0021658.1 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.8 bits (665), Expect = 9.4e-66
Identity = 140/184 (76.09%), Postives = 159/184 (86.41%), Query Frame = 0


Query: 8   LPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRL 67
           LPIT IL M  LINQS+   TQPINDTQFI TTCQ+TPYP LCLSSLS DA+AIHGS+RL
Sbjct: 6   LPITAILFM-ILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRL 65

Query: 68  ITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKY 127
           +T+AAL++ALTHT  ASS V SLA+SNAL PRE YAL+DCI+EF DSIEELKMAV ELK 
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAKSNALTPREGYALSDCIEEFGDSIEELKMAVEELKG 125

Query: 128 DDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLV 187
           +D  R++ +DI+TWVSAALTDD+TCMDGFA DAMNG VKES+RALVVDVAQ+TSIALSLV
Sbjct: 126 NDKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSLV 185

Query: 188 SQLK 192
            QLK
Sbjct: 186 RQLK 188

[Close]

BLAST of Tan0021658.1 vs. NCBI nr
Match: XP_022923321.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 250.4 bits (638), Expect = 1.3e-62
Identity = 133/184 (72.28%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 8   LPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRL 67
           LPIT I+ +  LINQS+   TQPINDTQFI TTCQ+TPYP LCLSSLS DA+AI  S+RL
Sbjct: 6   LPITAIVFV-ILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRL 65

Query: 68  ITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKY 127
           +T+AAL++ALTHT  ASS V SLA+SNA+  RE YAL+DCI+EF DS+EELKMAV  LK 
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKG 125

Query: 128 DDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLV 187
           +D  R++TEDI+TW+SAALTDD+TCMDGFAGDAMNG VKESM+A+VVDVAQ+TSIALSLV
Sbjct: 126 NDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185

Query: 188 SQLK 192
            QLK
Sbjct: 186 RQLK 188

[Close]

BLAST of Tan0021658.1 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 247.7 bits (631), Expect = 8.3e-62
Identity = 138/193 (71.50%), Postives = 161/193 (83.42%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSH-IAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAA 60
           M+S + PLP+  ILL+  LINQS+ IA +QPINDTQFI TTCQSTPYP LCLSSLS  AA
Sbjct: 1   MKSSYFPLPVKAILLI-LLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  AIHGSYRLITVAALSIALTHTRSASSAVASLAE-SNALAPRESYALNDCIDEFADSIEEL 120
            IH S  L+TVAAL++ALTHTRS SSA+ SLA+ SNAL PR+SY + DCI+EF DS+EEL
Sbjct: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120

Query: 121 KMAVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQ 180
           KMAV ELK ++ +R ETEDIRTWVSAALTDDDTCMDG  GDAMNG VKES++ +VV+VAQ
Sbjct: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180

Query: 181 MTSIALSLVSQLK 192
           +TSIALSLVS LK
Sbjct: 181 LTSIALSLVSLLK 192

[Close]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.2e-67
Identity = 140/192 (72.92%), Postives = 162/192 (84.38%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAA 60
           M+S + PLP+  ILL+  LINQS+IA +QP NDTQFI TTCQSTPYP LCLSSLS  AAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHGSYRLITVAALSIALTHTRSASSAVASLAE-SNALAPRESYALNDCIDEFADSIEELK 120
           IH SYRL+T+AAL++ALTHTRS SSAV SLA+ SNAL PRE Y + DCI+EF DS+EELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQM 180
           MA+ ELK ++  R ETEDIRTWVSAALTDDDTCMDG  GDAMNG +KES++A+VVDVAQ+
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSQLK 192
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 6.2e-63
Identity = 133/184 (72.28%), Postives = 157/184 (85.33%), Query Frame = 0


Query: 8   LPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRL 67
           LPIT I+ +  LINQS+   TQPINDTQFI TTCQ+TPYP LCLSSLS DA+AI  S+RL
Sbjct: 6   LPITAIVFV-ILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRL 65

Query: 68  ITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAELKY 127
           +T+AAL++ALTHT  ASS V SLA+SNA+  RE YAL+DCI+EF DS+EELKMAV  LK 
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAKSNAITTREGYALSDCIEEFGDSVEELKMAVEALKG 125

Query: 128 DDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLV 187
           +D  R++TEDI+TW+SAALTDD+TCMDGFAGDAMNG VKESM+A+VVDVAQ+TSIALSLV
Sbjct: 126 NDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185

Query: 188 SQLK 192
            QLK
Sbjct: 186 RQLK 188

[Close]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.0e-62
Identity = 138/193 (71.50%), Postives = 161/193 (83.42%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSH-IAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAA 60
           M+S + PLP+  ILL+  LINQS+ IA +QPINDTQFI TTCQSTPYP LCLSSLS  AA
Sbjct: 1   MKSSYFPLPVKAILLI-LLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  AIHGSYRLITVAALSIALTHTRSASSAVASLAE-SNALAPRESYALNDCIDEFADSIEEL 120
            IH S  L+TVAAL++ALTHTRS SSA+ SLA+ SNAL PR+SY + DCI+EF DS+EEL
Sbjct: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120

Query: 121 KMAVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQ 180
           KMAV ELK ++ +R ETEDIRTWVSAALTDDDTCMDG  GDAMNG VKES++ +VV+VAQ
Sbjct: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180

Query: 181 MTSIALSLVSQLK 192
           +TSIALSLVS LK
Sbjct: 181 LTSIALSLVSLLK 192

[Close]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.9e-56
Identity = 129/191 (67.54%), Postives = 153/191 (80.10%), Query Frame = 0


Query: 1   MESFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAA 60
           ME  + PLPIT ILL+  LIN S+      I+DTQFI  TCQ+TPYP LC SSLS DA +
Sbjct: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60

Query: 61  IHGSYRLITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKM 120
           +HGS R +T+AAL++ALTHTRSASS VASLA+SNALAP+E++AL DCI+E  DS+EEL+ 
Sbjct: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAKSNALAPQETWALRDCIEELGDSVEELQS 120

Query: 121 AVAELKYDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMT 180
           +VAELK  D +R ET+DIRTWVSAALTDDDTCM+G  GD   G VKES+R LVVDVAQ+T
Sbjct: 121 SVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLT 180

Query: 181 SIALSLVSQLK 192
           SIALSLVS LK
Sbjct: 181 SIALSLVSHLK 184

[Close]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 9.6e-40
Identity = 92/125 (73.60%), Postives = 108/125 (86.40%), Query Frame = 0


Query: 68  ITVAALSIALTHTRSASSAVASLAE-SNALAPRESYALNDCIDEFADSIEELKMAVAELK 127
           +T+AAL++ALTHTRS SSAV SLA+ SNAL PRE Y + DCI+EF DS+EELKMA+ ELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 128 YDDNARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSL 187
            ++  R ETEDIRTWVSAALTDDDTCMDG  GDAMNG +KES++A+VVDVAQ+TSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 188 VSQLK 192
           VS LK
Sbjct: 121 VSLLK 125

[Close]

BLAST of Tan0021658.1 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 4.1e-27
Identity = 70/178 (39.33%), Postives = 111/178 (62.36%), Query Frame = 0


Query: 13  ILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAA 72
           I+ M  L++ +    T    +T+F+ ++C  T YP+LC SSLS  A+ I  S +L+  AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 73  LSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAVAEL--KYDDN 132
           L+I L   +  S+ +  L+ S  L P+E  A+ DC++E  D++EEL+ ++ E+      N
Sbjct: 80  LNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 133 ARVETEDIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVS 189
             V   DI+TWVSAALTD +TC DGF G+ M+G+VK  +R  ++ +A +TS AL+L++
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALIN 196

[Close]

BLAST of Tan0021658.1 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 9.5e-24
Identity = 62/159 (38.99%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 36  FINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAALSIALTHTRSASSAVASLAESNA 95
           FI ++C++T YP LC+ SLSV A  I  S + +   A+++ L+  +S    V+ L     
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 96  LAPRESYALNDCIDEFADSIEELKMAVAELKYDDNARVETE------DIRTWVSAALTDD 155
           L  RE  A+ DC++E  D+++ L  +V ELK   +A+ + +      + +TW SAALTD+
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDE 157

Query: 156 DTCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVS 189
           +TC DGF+G  M+GR+K S+RA +++V   TS ALSL++
Sbjct: 158 NTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

[Close]

BLAST of Tan0021658.1 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 6.2e-23
Identity = 77/189 (40.74%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  ITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAI-HGSYRLI 69
           I  ++L+   I QS  A        QFI ++CQ+T YP LC+ +LS  A  I H + + +
Sbjct: 7   IFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDL 66

Query: 70  TVAALSIALTHTRSASSAVASLA-ESNALAPRESYALNDCIDEFADSIEELKMAVAELKY 129
              AL I+L   +S +  VA L  E+     RE  A+ DCI+   +S++ L  +V EL  
Sbjct: 67  AQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELAR 126

Query: 130 DDNARVETED-------IRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDVAQMT 189
             +A V +ED       ++TWVSAALTD+ TC+DGF+  AM G+VK  +R  VV VAQ+T
Sbjct: 127 AGHA-VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 186

[Close]

BLAST of Tan0021658.1 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-22
Identity = 62/192 (32.29%), Postives = 107/192 (55.73%), Query Frame = 0


Query: 3   SFHVPLPITTILLMFFLINQSHIAKTQPINDTQFINTTCQSTPYPQLCLSSLSVDAAAIH 62
           +F + L +  + L    I  +   + +   DT+FI  +C++T YP  C  SLS  A+ I 
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 63  GSYRLITVAALSIALTHTRSASSAVASLAESNALAPRESYALNDCIDEFADSIEELKMAV 122
              R +   AL++++   +SA + V+ + +   +  R+  A+ DC++E  D+++ L  ++
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 123 AELKYDDNARVETE------DIRTWVSAALTDDDTCMDGFAGDAMNGRVKESMRALVVDV 182
            ELK+ +      +      ++RTW SAALTD+  CMDGF G AM G +K  +R  +V V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 183 AQMTSIALSLVS 189
           A+ TS AL+L++
Sbjct: 184 AEETSNALALIN 195

[Close]

BLAST of Tan0021658.1 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-21
Identity = 63/158 (39.87%), Postives = 99/158 (62.66%), Query Frame = 0


Query: 36  FINTTCQSTPYPQLCLSSLSVDAAAIHGSYRLITVAALSIALTHTRSASSAVASLAESNA 95
           +I T+C  T Y  +C +SLS  A+ I  + + + V AL++ L+  +SAS  V +++    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 96  LAPRESYALNDCIDEFADSIEELKMAVAEL---KYDDNARVE--TEDIRTWVSAALTDDD 155
           L   E  A+ DC++E  DS+  L+ ++ EL    Y D+A+ E    D+ TWVSAALT+DD
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 156 TCMDGFAGDAMNGRVKESMRALVVDVAQMTSIALSLVS 189
           TCMDGF+   +   VK+ +R  VV+VA++TS AL+L++
Sbjct: 267 TCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALIN 302

The following BLAST results are available for this feature:

BLAST of Tan0021658.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	2.4e-24	41.07	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI72	8.7e-22	40.74	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	1.5e-21	32.29	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	1.6e-20	39.87	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	2.8e-20	35.87	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]

BLAST of Tan0021658.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876812.1	1.3e-67	74.35	21 kDa protein-like [Benincasa hispida]	[more]
XP_008448897.1	6.5e-67	72.92	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_023551923.1	9.4e-66	76.09	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022923321.1	1.3e-62	72.28	21 kDa protein-like [Cucurbita moschata]	[more]
XP_004147977.1	8.3e-62	71.50	21 kDa protein [Cucumis sativus]	[more]

BLAST of Tan0021658.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BLP3	3.2e-67	72.92	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A6J1E6H9	6.2e-63	72.28	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A0A0L1P3	4.0e-62	71.50	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A6J1DFW8	1.9e-56	67.54	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A5D3D7I0	9.6e-40	73.60	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of Tan0021658.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	4.1e-27	39.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	9.5e-24	38.99	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	6.2e-23	40.74	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	1.1e-22	32.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	1.2e-21	39.87	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..186 e-value: 2.7E-40 score: 149.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..190 e-value: 2.4E-33 score: 112.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..186 e-value: 1.3E-34 score: 119.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..191 e-value: 7.7E-37 score: 128.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 35..190
None	No IPR available	PANTHER	PTHR31080:SF96	21 KDA PROTEIN-LIKE	coord: 10..188
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 10..188
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 39..190 e-value: 2.45986E-35 score: 119.85

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0021658	Tan0021658	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0021658.1-exon	Tan0021658.1-exon-LG01:22213237..22213812	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0021658.1-cds	Tan0021658.1-cds-LG01:22213237..22213812	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0021658.1	Tan0021658.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity