Tan0012759.2 (mRNA) Snake gourd v1

Overview


Name	Tan0012759.2
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	very-long-chain enoyl-CoA reductase-like
Location	LG11: 6161098 .. 6165320 (-)
Sequence length	2121
RNA-Seq Expression	Tan0012759.2
Synteny	Tan0012759.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATCAAAATCACCGCATATACTCTCCCAATACATAAATGCAGAGATGGGTCACCATAAATGACATGTGTTGCTTTTAGATACCCTTAGATTTTTCTGTTTACTCATCATTTCCTCTTCTTAAGTCACTACCAATTCTCAGATTTTTGGAGTGATGATACAGACACCAACCCCTTCTCGTCACTAATCCTTAACCAAATTTTGCTGCATTTAAAAATTTAGGTTGACGTTCATCACAAACAGAATGATGAGTCGAATTGTGAATTTGTAATTAGGAGATGATTTGGGACACGGCTCATCCGTCTTCGGAATTAGCTATCCTACCCCAACCGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCTACACCAAACCCAGAAAACTTCTACCAGTTTTTAATAGAATTCATTTGGGGATTTCATCATATAGCCATTTCTTGCTTCTGGGTTCTCTCATTCTGCTCTTTCAGGATCTGGGTTGAAATAATACGGCTACTTGCCCAACTGGGAGTCTCTGATTTGACTGCGAAGATGAAGGTCTCGGTGGTTTCTCGCAGTGGGAGAGAGGTTGTTAAGGGTGGCATTCAACTGAGCGATTCTGTAAGTGTTTGGATTTTGATTGAATTACTGTTTGTTTTTCATTTCCCTTTTTCGATTCTTGGAAAAGAAGAAAACATGAGAAGAGTACAACTCGTTTTGGTGGCTGTGTTCAGTTTATAGACCTATCTATTTGGCTTCTTACAGATGAGTAGATGATGGGACTCTTAGGTTTCATGGAAATTGTTGTTTTATTTTTTGTAGGCTTCGGTGGCCGATCTGCAGGATGCTATTTATAAGCGAAGTAAGTGCAAGATAGCTGATTTTTTCCTTTAATTGTTGCAGACATGCTCTCATTAATATTAGCTTAGATTTTTTAGATTGGTTAGCTTAGAATTGACATTTTTTATAGGAGTGAGTTATAGAACTGGAGTTTGCAGTTGTGCTACCATGTCTACTTGTGTTTTGCCCCATTTTGTAGTTGTTAAAAGGATGAATCTAAAAGATGAAGTTTAGCCTAGCCTAACAGTTCAAGCTTTTTGACTGCAACGACTTAGAGAGGTGGGGATTCAAAGCTCCGATCTTTTGACCTTATAATGCCTCAACAATTTAAGCTATCGGGTATGGAATTGCTGAGGAATCAAAATCTTGTTAACAAGTTCATAGATAGTGAAGCATATCAAGAAGTCCTCTTTTTTTTTTTTTTAATAACTGGGAGTCAAAATTTTGCTCCACTGTACTCGGGGCACCCATGTCCGCCCTAAGGTGTGGACCTGGGAGACATAAAAGTTTTTATTTATTAAGCTCACTCGAGGGTTCGAGACCTCTAAGCCCGTATGACCAAGAGATCCTAAGCCCTTACCAACAAGGTCGCCCCTTGTGGGCTAAAAAAGAAGTTCATTGTTTCCTATCTTATGCTTCCAGAAAATCAGTCTTTTTATTACTAGTGGTAAGGAAAGATGGTATTGGGAATTAATCTTCACTCGGATATGCTGATTTCTTCTGTTTTTTCCCCCTTGACCAAATGTGTATCGTTCCGCCTTTATTATGTTGCATAGAGTTACCAGGCAAAGAACGAAATGATTTTGTTATTGCTGGTTAAGTTCTTTCTTTCTTGGATGGGGTGGGAGTGCAAAATAAAATTTTGGAGTTCAATAAGATTGTCAGTGCACCTCAATAGCTTTAAAAATAAATATGCATATCAGTGTGGTTGTTCTGTGAGAACTAATGGAAAGTACCTTTACTCTTAATTTTCTAACAAAATGCATACATGGCATTCTAGATGCTACATAGCAATCCCTGTCCTTAGCAAAGTTCTTTTTGTCAGAAACTTTAGAGAAGAAATTCTCATTGTCTAAATATTGAATGAAGAGGTATCTTTTGTTAGTTTTTGCAATTACTGCATAAATTTGTACTTCTAACAACAGGGAGTGCATTAGATTAGCTGCTAAATCCTAATGGTTATCTTATTACATACTGGTATACTTTGTAGTTCTTTTCTTTTTTAACAATTATTTCTGCATTTGCATTATGCCTTTAGGCCTTTGGTCATTGTAGCTTGGCTGAAGAATTGTCTTATTAATAAACGAAACATGCTTTGCAGGCTTTTGTAGATCCTTTTGCATCATCTACTTGATATTTCCTCTGAACTTTGTCATCTTAGCTAAGATTAAACATAAAGCACTTGACGAGAATTACTTTACGACCTTTACTATTATTAGAATTCCTCAACTCCACTATGAGTTCTATCAAGAAGGAAAAAACAAAAAGAAATCTTAAACGACCTGATAGTGTCTATTGTATCTTAGAACTTAAACGGTTGACATATTTGTCTGATATTGTTGTATGGTTCTTAAGAAGCGTCAAGTTGCCAGTCATTTAACATGCATATATGTAAATCGACGCAAGTTTTTTTTATGTAAAGTGCAGCTACCTAATTTACTTGTTTTATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCGAAGGAGAGGCCTACTGTTCTTAACTATAAGAAAAGCCTTCGAGAGTATTGTAGCCACAACTCGGACAACATAACTGTCGTCTTCAAGGACTTGGGGCCACAAGTTTCTTATAGTACACTCTTCTTTTTTGAATACTTGGGTCCATTGATCCTCTACCCCATCTTTTACTACTTCCCCGTATATCAGTTCTTTGGCTACAAGGCAGAACGTGTCGTCCATCCAGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTATTTCAAGCGCATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCGCTTTCGAATGTGTTCCGAAACTGTGCTTACTACTGGTCATTTGGCTCGTATATTGCATATTATGTTAATCACCCACTTTATACCCCTGTTGGCGACCCTCAAATGAAGATTGGCTTTGCATTTGGTTTGCTTTGTCAAGTGTCGAACTTCTACTGTCACATCTTGCTGAGAAAATTGCGCAGTCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTTGCTGGTTATGTCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCTCTTGCAAAGCACCGTCGCCTCAAGAAGGTATCATCTAACTTATTTTTTCTAGTGGTTTAAGCTTTTAGGTTTAGGTAATTCAATGATTTCTGATACCGTGTCAAATCACTTCTAAACAGAAGAATTTTAAGTTGATAGGTTAGTGTCTATTAATCTTTTTTATATACTGTGAAGTGTTAACACTACTTCTCTAGTGGACTTAGGAACTAACCCAAGATCCAATAGATGGATTATCGATATTAATTAGAGGAGAAGTGGTACTACAGCAGATTGAAAATAGTACCTCTTGCTTTGATACTATATGAAATCACTACTAAATTTCTATATTCTTAATAGTTAGCTTTGCTACGCTTTGGTTTGAACGTGATGCTCTGCGTTCAAACGTGAGTATAACTTCTCTCTCGAGTCTTGACAACTCGAGATTCCCATCACATTTTTGGATAATAGGCTAGAGGCGTTGGAATGGACTGAAGTTTTTGGCTTTGCAAGTTCCTTAGTTTTGAAGATAAAAGATGCCATTTTGGTAGATTCCAAGTACTTTAGTTGTTATTTTGCCTCTCATTTTGACAAATTGTTTTCCCTATCTGCAGCTGTTTGATGGGAAGGAGGGAAAGCCAAAGTACCCACGCAGATGGGTGATTCTTCCTCCATTTTTGTAGAAGGATTGGGCTGCCTCAACAGCTCCTTATTTATCTCCTCAGAATCCATTCAAGTTTAAAATGGCCCTTGAAAGACACGACACGACATCATTTTTTCAATGCAATTTATGTTCTTCGTCGTTGTCTTAACATTATGGACAATGGTCCTTTTGTTTTGTGTTGTTAGTGGATTGTTGGTGACTGATGATTCCATGTTTTTCTCTTCATAGCCTTTCGAATTCTCTCCTTTTCTGTTAGTTTGACTTTGAGGACATTCTTTCAACTGCTCTTTTTTATTGATATATTTTAGATGCTTCTAAATTTGTTGTTCTAATGGATTTGGTGCCACAT

mRNA sequence

TATCAAAATCACCGCATATACTCTCCCAATACATAAATGCAGAGATGGGTCACCATAAATGACATGTGTTGCTTTTAGATACCCTTAGATTTTTCTGTTTACTCATCATTTCCTCTTCTTAAGTCACTACCAATTCTCAGATTTTTGGAGTGATGATACAGACACCAACCCCTTCTCGTCACTAATCCTTAACCAAATTTTGCTGCATTTAAAAATTTAGGTTGACGTTCATCACAAACAGAATGATGAGTCGAATTGTGAATTTGTAATTAGGAGATGATTTGGGACACGGCTCATCCGTCTTCGGAATTAGCTATCCTACCCCAACCGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCTACACCAAACCCAGAAAACTTCTACCAGTTTTTAATAGAATTCATTTGGGGATTTCATCATATAGCCATTTCTTGCTTCTGGGTTCTCTCATTCTGCTCTTTCAGGATCTGGGTTGAAATAATACGGCTACTTGCCCAACTGGGAGTCTCTGATTTGACTGCGAAGATGAAGGTCTCGGTGGTTTCTCGCAGTGGGAGAGAGGTTGTTAAGGGTGGCATTCAACTGAGCGATTCTGCTTCGGTGGCCGATCTGCAGGATGCTATTTATAAGCGAAGTAAGTGCAAGATAGCTGATTTTTTCCTTTAATTGTTGCAGACATGCTCTCATTAATATTAGCTTAGATTTTTTAGATTGGTTAGCTTAGAATTGACATTTTTTATAGGAGTGAGTTATAGAACTGGAGTTTGCAGTTGTGCTACCATGTCTACTTGTGTTTTGCCCCATTTTGTAGTTGTTAAAAGGATGAATCTAAAAGATGAAGTTTAGCCTAGCCTAACAGTTCAAGCTTTTTGACTGCAACGACTTAGAGAGGTGGGGATTCAAAGCTCCGATCTTTTGACCTTATAATGCCTCAACAATTTAAGCTATCGGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCGAAGGAGAGGCCTACTGTTCTTAACTATAAGAAAAGCCTTCGAGAGTATTGTAGCCACAACTCGGACAACATAACTGTCGTCTTCAAGGACTTGGGGCCACAAGTTTCTTATAGTACACTCTTCTTTTTTGAATACTTGGGTCCATTGATCCTCTACCCCATCTTTTACTACTTCCCCGTATATCAGTTCTTTGGCTACAAGGCAGAACGTGTCGTCCATCCAGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTATTTCAAGCGCATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCGCTTTCGAATGTGTTCCGAAACTGTGCTTACTACTGGTCATTTGGCTCGTATATTGCATATTATGTTAATCACCCACTTTATACCCCTGTTGGCGACCCTCAAATGAAGATTGGCTTTGCATTTGGTTTGCTTTGTCAAGTGTCGAACTTCTACTGTCACATCTTGCTGAGAAAATTGCGCAGTCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTTGCTGGTTATGTCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCTCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAAGCCAAAGTACCCACGCAGATGGGTGATTCTTCCTCCATTTTTGTAGAAGGATTGGGCTGCCTCAACAGCTCCTTATTTATCTCCTCAGAATCCATTCAAGTTTAAAATGGCCCTTGAAAGACACGACACGACATCATTTTTTCAATGCAATTTATGTTCTTCGTCGTTGTCTTAACATTATGGACAATGGTCCTTTTGTTTTGTGTTGTTAGTGGATTGTTGGTGACTGATGATTCCATGTTTTTCTCTTCATAGCCTTTCGAATTCTCTCCTTTTCTGTTAGTTTGACTTTGAGGACATTCTTTCAACTGCTCTTTTTTATTGATATATTTTAGATGCTTCTAAATTTGTTGTTCTAATGGATTTGGTGCCACAT

Coding sequence (CDS)

ATGCCTCAACAATTTAAGCTATCGGCCAAAAAGTTCTACCCTGCTAGGCAACGGTTAACTCTTCCCGTTCAACCTGGTTCGAAGGAGAGGCCTACTGTTCTTAACTATAAGAAAAGCCTTCGAGAGTATTGTAGCCACAACTCGGACAACATAACTGTCGTCTTCAAGGACTTGGGGCCACAAGTTTCTTATAGTACACTCTTCTTTTTTGAATACTTGGGTCCATTGATCCTCTACCCCATCTTTTACTACTTCCCCGTATATCAGTTCTTTGGCTACAAGGCAGAACGTGTCGTCCATCCAGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTATTTCAAGCGCATCATGGAAACATTTTTCGTGCATAGGTTCAGCCACGCAACCTCACCGCTTTCGAATGTGTTCCGAAACTGTGCTTACTACTGGTCATTTGGCTCGTATATTGCATATTATGTTAATCACCCACTTTATACCCCTGTTGGCGACCCTCAAATGAAGATTGGCTTTGCATTTGGTTTGCTTTGTCAAGTGTCGAACTTCTACTGTCACATCTTGCTGAGAAAATTGCGCAGTCCTGAGGGTAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTTGCTGGTTATGTCTTTCTTGTGGTTGCTACTCTTATCATGACGAACTGGGCTCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAAGCCAAAGTACCCACGCAGATGGGTGATTCTTCCTCCATTTTTGTAG

Protein sequence

MPQQFKLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL

Homology

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BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.0e-139
Identity = 226/282 (80.14%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MPQQFKLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGP 60
           + + F   AKKFYP+RQRLTLPV PGSK++P VLN KKSL+EYC  N++++TVVFKDLG 
Sbjct: 29  LQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGA 88

Query: 61  QVSYSTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIME 120
           QVSY TLFFFEYLGPL++YP+FYYFPVY+F GY  + V+HPVQTYAMYYWCFHYFKRI+E
Sbjct: 89  QVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILE 148

Query: 121 TFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQV 180
           TFFVHRFSHATSP+ NVFRNCAYYWSFG+YIAYYVNHPLYTPV D QMKIGF FGL+CQV
Sbjct: 149 TFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQV 208

Query: 181 SNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFL 240
           +NFYCHILL+ LR P G GGYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AGYVFL
Sbjct: 209 ANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFL 268

Query: 241 VVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
            VA LIMTNWAL KH RL+K+FDGK+GKPKYPRRWVILPPFL
Sbjct: 269 AVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.6e-51
Identity = 105/237 (44.30%), Postives = 144/237 (60.76%), Query Frame = 0


Query: 46  HNSDNITVVFKDLGPQVSYSTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTY 105
           H   + T+ FKDLGPQ+S+S +F  EY GPL +YPIFY+        Y  +      Q  
Sbjct: 72  HVGADSTLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFL---SNLIYGTDSPKSFAQKV 131

Query: 106 AMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD 165
           A+  +  HY KRI ET FVHRFSH T P+ N+F+NC+YYW   + ++Y+VNHPLYT    
Sbjct: 132 ALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYFVNHPLYTEAPI 191

Query: 166 PQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWV 225
            ++ +G    ++ +V N+ CHI LR LR P G+   +IP+G LF  V+C NYT EI  W+
Sbjct: 192 ERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFVSCPNYTVEILSWI 251

Query: 226 GFNIATQTVAGYVFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           GF+I TQT+  ++F ++    M  WA+ KHRR +K F       KYP+   IL PFL
Sbjct: 252 GFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFG-----DKYPKSRKILIPFL 299

[Close]

BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot
Match: Q9N5Y2 (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.4e-46
Identity = 106/285 (37.19%), Postives = 153/285 (53.68%), Query Frame = 0


Query: 6   KLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYS 65
           +++ KK     +R  L V+P  K +P   + K S       +S    +  +DLGPQ+++ 
Sbjct: 35  RIAQKKLKLTEERQALRVEP--KGKPLADDQKLS---DLGLSSQKAVLYVRDLGPQIAWK 94

Query: 66  TLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVH 125
           T+F  EY GPL +YP+FY  P + +        +HP    A + W FHY KR+ ET F+H
Sbjct: 95  TVFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIH 154

Query: 126 RFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDPQMKIGFAFGLLCQVSNF 185
           RF ++T P  N+ +NC+YYW F +++AY+VNHPL+TP   GD Q+  G A  ++ +  N 
Sbjct: 155 RFGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNL 214

Query: 186 YCHILLRKLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGY 245
             HILLR LR P G    +IPK        LFN V+C NYT E+  W+ F+I  Q++   
Sbjct: 215 SIHILLRNLR-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAI 274

Query: 246 VFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           +F       M  WA  KHR   K F      P YP+    + PF+
Sbjct: 275 IFTTAGFAQMAIWAQGKHRNYLKEF------PDYPKNRKAIVPFV 307

[Close]

BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.7e-45
Identity = 116/288 (40.28%), Postives = 158/288 (54.86%), Query Frame = 0


Query: 5   FKLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSY 64
           F  +  ++YPARQ L L  +  S +   VL                 T+ F+DLG Q+S+
Sbjct: 36  FTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISW 95

Query: 65  STLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVV--HPVQTYAMYYWCFHYFKRIMETF 124
            T+F  EY GPL +Y + +YF V   +G+K +     H V   A     FHY KR++ET 
Sbjct: 96  VTVFLTEYAGPLFIY-LLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIKRLLETL 155

Query: 125 FVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDPQMKIGFAFGLLCQV 184
           FVHRFSH T PL N+F+NC YYW F +++AYY+NHPLYTP   G  Q+K+  A  ++CQ+
Sbjct: 156 FVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQL 215

Query: 185 SNFYCHILLRKLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTV 244
            NF  H+ LR LR P G+   +IP        +LF +V+C NYT E+  W+GF I TQ +
Sbjct: 216 GNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCL 275

Query: 245 AGYVFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
              +F +V    MT WA  KHR   K F        YP   + + PFL
Sbjct: 276 PVALFSLVGFTQMTIWAKGKHRSYLKEF------RDYPPLRMPIIPFL 307

[Close]

BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.8e-45
Identity = 117/288 (40.62%), Postives = 157/288 (54.51%), Query Frame = 0


Query: 5   FKLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSY 64
           F  +  ++YPARQ L L  +  S +   VL                 T+ F+DLG Q+S+
Sbjct: 36  FTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV--------GTTATLYFRDLGAQISW 95

Query: 65  STLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVV--HPVQTYAMYYWCFHYFKRIMETF 124
            T+F  EY GPL +Y + +YF V   +G K +     H V   A     FHY KR++ET 
Sbjct: 96  VTVFLTEYAGPLFIY-LLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIKRLLETL 155

Query: 125 FVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTP--VGDPQMKIGFAFGLLCQV 184
           FVHRFSH T PL N+F+NC YYW F +++AYY+NHPLYTP   G  Q+K+  A  ++CQ+
Sbjct: 156 FVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAIFVICQL 215

Query: 185 SNFYCHILLRKLRSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTV 244
            NF  H+ LR LR P G+   +IP        +LF +V+C NYT E+  W+GF I TQ V
Sbjct: 216 GNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCV 275

Query: 245 AGYVFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
              +F +V    MT WA  KHR   K F        YP   + + PFL
Sbjct: 276 PVALFSLVGFTQMTIWAKGKHRSYLKEF------RDYPPLRMPIIPFL 307

[Close]

BLAST of Tan0012759.2 vs. NCBI nr
Match: XP_022921854.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita moschata])

HSP 1 Score: 558.1 bits (1437), Expect = 4.2e-155
Identity = 263/273 (96.34%), Postives = 270/273 (98.90%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLNYKKSL+EYCS+NSDNITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQV NFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+P+YPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. NCBI nr
Match: XP_022990641.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita maxima])

HSP 1 Score: 557.4 bits (1435), Expect = 7.2e-155
Identity = 263/273 (96.34%), Postives = 269/273 (98.53%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPG KERPTVLNYKKSLREYCS+NSDNITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGLKERPTVLNYKKSLREYCSNNSDNITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQV NFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+P+YPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. NCBI nr
Match: KAG6602374.1 (Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.0 bits (1434), Expect = 9.4e-155
Identity = 262/273 (95.97%), Postives = 270/273 (98.90%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLNYKK+L+EYCS+NSDNITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNYKKTLKEYCSNNSDNITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQV NFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+P+YPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. NCBI nr
Match: XP_038890322.1 (very-long-chain enoyl-CoA reductase [Benincasa hispida])

HSP 1 Score: 556.2 bits (1432), Expect = 1.6e-154
Identity = 262/273 (95.97%), Postives = 268/273 (98.17%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLP+QPGSKERPTVLNYKKSLREYCS NSDNITV+FKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPIQPGSKERPTVLNYKKSLREYCSDNSDNITVIFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           +EYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  WEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD QMKIGFAFGLLCQ+SNFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGYVFLVVA LIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+PKYPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. NCBI nr
Match: XP_004142757.1 (very-long-chain enoyl-CoA reductase [Cucumis sativus] >KGN51310.1 hypothetical protein Csa_008203 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 4.7e-154
Identity = 264/273 (96.70%), Postives = 267/273 (97.80%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLN KKSLREYCS NSD+ITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD QMKIGFAFGLLCQVSNFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGYVFLVVA LIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+PKYPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL
Match: A0A6J1E1Q3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111429991 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.0e-155
Identity = 263/273 (96.34%), Postives = 270/273 (98.90%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLNYKKSL+EYCS+NSDNITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQV NFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+P+YPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL
Match: A0A6J1JNI3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111487465 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.5e-155
Identity = 263/273 (96.34%), Postives = 269/273 (98.53%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPG KERPTVLNYKKSLREYCS+NSDNITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGLKERPTVLNYKKSLREYCSNNSDNITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLY+PVGD QMKIGFAFGLLCQV NFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGY+FLVVATLIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+P+YPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL
Match: A0A0A0KNT8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517090 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.3e-154
Identity = 264/273 (96.70%), Postives = 267/273 (97.80%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLN KKSLREYCS NSD+ITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGYKAERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD QMKIGFAFGLLCQVSNFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGYVFLVVA LIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+PKYPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL
Match: A0A5A7T2H3 (Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00470 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 6.6e-154
Identity = 263/273 (96.34%), Postives = 267/273 (97.80%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLN KKSLREYCS NSD+ITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGY+AERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD QMKIGFAFGLLCQVSNFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGYVFLVVA LIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+PKYPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL
Match: A0A1S3C9G7 (very-long-chain enoyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103498153 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 6.6e-154
Identity = 263/273 (96.34%), Postives = 267/273 (97.80%), Query Frame = 0


Query: 10  KKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGPQVSYSTLFF 69
           KKFYPARQRLTLPVQPGSKERPTVLN KKSLREYCS NSD+ITVVFKDLGPQVSY TLFF
Sbjct: 38  KKFYPARQRLTLPVQPGSKERPTVLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFF 97

Query: 70  FEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 129
           FEYLGPLILYPIFYYFPVYQFFGY+AERV+HPVQTYAMYYWCFHYFKRIMETFFVHRFSH
Sbjct: 98  FEYLGPLILYPIFYYFPVYQFFGYQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSH 157

Query: 130 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQVSNFYCHILL 189
           ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGD QMKIGFAFGLLCQVSNFYCHILL
Sbjct: 158 ATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILL 217

Query: 190 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTN 249
           RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQT AGYVFLVVA LIMTN
Sbjct: 218 RKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTN 277

Query: 250 WALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           WALAKHRRLKKLFDGKEG+PKYPRRWVILPPFL
Sbjct: 278 WALAKHRRLKKLFDGKEGRPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 1.4e-140
Identity = 226/282 (80.14%), Postives = 254/282 (90.07%), Query Frame = 0


Query: 1   MPQQFKLSAKKFYPARQRLTLPVQPGSKERPTVLNYKKSLREYCSHNSDNITVVFKDLGP 60
           + + F   AKKFYP+RQRLTLPV PGSK++P VLN KKSL+EYC  N++++TVVFKDLG 
Sbjct: 29  LQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGA 88

Query: 61  QVSYSTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIME 120
           QVSY TLFFFEYLGPL++YP+FYYFPVY+F GY  + V+HPVQTYAMYYWCFHYFKRI+E
Sbjct: 89  QVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILE 148

Query: 121 TFFVHRFSHATSPLSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKIGFAFGLLCQV 180
           TFFVHRFSHATSP+ NVFRNCAYYWSFG+YIAYYVNHPLYTPV D QMKIGF FGL+CQV
Sbjct: 149 TFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQV 208

Query: 181 SNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFL 240
           +NFYCHILL+ LR P G GGYQIP+GFLFNIVTCANYTTEIYQW+GFNIATQT+AGYVFL
Sbjct: 209 ANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFL 268

Query: 241 VVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
            VA LIMTNWAL KH RL+K+FDGK+GKPKYPRRWVILPPFL
Sbjct: 269 AVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL 310

[Close]

BLAST of Tan0012759.2 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.2e-12
Identity = 57/210 (27.14%), Postives = 96/210 (45.71%), Query Frame = 0


Query: 74  GPLILYPIFYYFPVYQFFGYKAERVVHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSP 133
           G L+LY   +      FF   ++ +   +   A+     H+FKR+ E  F+H++S   + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 134 LSNVFRNCAYYWSFGSYIAYYVNHPLYTPVGDPQMKI-GFAFGLLCQVSNFYCHILLRKL 193
           + +     + Y+S  + + Y  N  L        MK+ G    ++  V N Y H+LL KL
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 194 RSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYVFLVVATLIMTNWAL 253
           R  +G   Y+IPKG LF+I+ C +Y  EI  +  F + +QT+  + F +   L +   + 
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 254 AKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
           A        FD       +P+    L PF+
Sbjct: 248 ATRTWYLSKFD------DFPKHIKALIPFV 267

[Close]

BLAST of Tan0012759.2 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.0e-06
Identity = 34/115 (29.57%), Postives = 56/115 (48.70%), Query Frame = 0


Query: 172 FAFGLLCQVSNFYCHI----LLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGF 231
           F  G++  ++  Y +I     L +L+  E  GGY IP+G  F +V+C NY  E  +W+G+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLKK-ENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 232 NIATQTVAGYVFLVVATLIMTNWALAKHRRLKKLFDGKEGKPKYPRRWVILPPFL 283
            + T + AG  F +     +   A A H+     F     K +YP+    + PF+
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPFV 261

The following BLAST results are available for this feature:

BLAST of Tan0012759.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U2	2.0e-139	80.14	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ...	[more]
Q55C17	2.6e-51	44.30	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
Q9N5Y2	4.4e-46	37.19	Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=...	[more]
Q9NZ01	1.7e-45	40.28	Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1	[more]
Q64232	2.8e-45	40.63	Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S...	[more]

BLAST of Tan0012759.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022921854.1	4.2e-155	96.34	very-long-chain enoyl-CoA reductase-like [Cucurbita moschata]	[more]
XP_022990641.1	7.2e-155	96.34	very-long-chain enoyl-CoA reductase-like [Cucurbita maxima]	[more]
KAG6602374.1	9.4e-155	95.97	Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_038890322.1	1.6e-154	95.97	very-long-chain enoyl-CoA reductase [Benincasa hispida]	[more]
XP_004142757.1	4.7e-154	96.70	very-long-chain enoyl-CoA reductase [Cucumis sativus] >KGN51310.1 hypothetical p...	[more]

BLAST of Tan0012759.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1Q3	2.0e-155	96.34	very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JNI3	3.5e-155	96.34	very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A0A0KNT8	2.3e-154	96.70	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G5170...	[more]
A0A5A7T2H3	6.6e-154	96.34	Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C9G7	6.6e-154	96.34	very-long-chain enoyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103498153 PE=3...	[more]

BLAST of Tan0012759.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55360.1	1.4e-140	80.14	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	3.2e-12	27.14	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	2.0e-06	29.57	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 131..281 e-value: 8.6E-28 score: 97.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 171..252 score: 21.960117
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 8..282
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 8..282

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0012759	Tan0012759	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0012759.2-three_prime_utr	Tan0012759.2-three_prime_utr-LG11:6161098..6161427	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0012759.2-exon	Tan0012759.2-exon-LG11:6161098..6161496	exon
Tan0012759.2-exon	Tan0012759.2-exon-LG11:6162060..6162814	exon
Tan0012759.2-exon	Tan0012759.2-exon-LG11:6164153..6164509	exon
Tan0012759.2-exon	Tan0012759.2-exon-LG11:6164711..6165320	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0012759.2-cds	Tan0012759.2-cds-LG11:6161428..6161496	CDS
Tan0012759.2-cds	Tan0012759.2-cds-LG11:6162060..6162814	CDS
Tan0012759.2-cds	Tan0012759.2-cds-LG11:6164153..6164177	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0012759.2-five_prime_utr	Tan0012759.2-five_prime_utr-LG11:6164178..6164509	five_prime_UTR
Tan0012759.2-five_prime_utr	Tan0012759.2-five_prime_utr-LG11:6164711..6165320	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0012759.2	Tan0012759.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0016631	enoyl-[acyl-carrier-protein] reductase activity
molecular_function	GO:0004318	enoyl-[acyl-carrier-protein] reductase (NADH) activity