Tan0012058.1 (mRNA) Snake gourd v1

BLAST of Tan0012058.1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.2e-127
Identity = 250/504 (49.60%), Postives = 338/504 (67.06%), Query Frame = 0

Query: 39  AIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP--------RSF 98
           A+ +  LFLT  + S    SLS P    T + DV +S ++ Q  LS++P        +  
Sbjct: 11  AVVTLSLFLTTTDAS--SRSLSTP--PKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 70

Query: 99  SDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLEL 158
           S SDPV  +S    +S L+L LH R +     +KDY+SL  +RL RD+ RV  +   +  
Sbjct: 71  SLSDPVFFNS----SSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRF 130

Query: 159 ALNGGQKLDGPALRPNF----------LTAPVVSGQSQGSGEYFARISVGQPARSFYLVP 218
           A+ G  + D   L+P +          LT PVVSG SQGSGEYF+RI VG PA+  YLV 
Sbjct: 131 AVEGVDRSD---LKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVL 190

Query: 219 DTGSDITWLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCV 278
           DTGSD+ W+QC PC++   CY+QSDP+F+P SSS+Y  L+C++ QC  L+   C+S  C+
Sbjct: 191 DTGSDVNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCL 250

Query: 279 YQVSYGDGSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLS 338
           YQVSYGDGSFT G+ AT+T+TFGNS  + N+ +GCGHDNEGLF GAAGL+GLGGG LS++
Sbjct: 251 YQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSIT 310

Query: 339 SQLKASSFSYCLVDRDSDSSSTLEFNTGR-PSDSLTSPLLRNNRFQSYRYVAVTGMSVGG 398
           +Q+KA+SFSYCLVDRDS  SS+L+FN+ +      T+PLLRN +  ++ YV ++G SVGG
Sbjct: 311 NQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGG 370

Query: 399 KPLPISSTRFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPP-ASGISPF 458
           + + +    F++D SG GG+I+D GT +TRL +  Y SLR+AF+KL  NL   +S IS F
Sbjct: 371 EKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLF 430

Query: 459 DTCYNLAGQSNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGS 518
           DTCY+ +  S V+VPTVAF  + G SL LPA NYLI +D +GT+C AF  T+SSLSIIG+
Sbjct: 431 DTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGN 490

Query: 519 FQQQGIRVSYDLVNSLVGFSSKKC 523
            QQQG R++YDL  +++G S  KC
Sbjct: 491 VQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Tan0012058.1 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.8e-99
Identity = 192/430 (44.65%), Postives = 263/430 (61.16%), Query Frame = 0

Query: 99  HSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALNGGQKL 158
           H     +S  TL L  R       Y+++   + AR+ RD  RV ++ R +      G+ +
Sbjct: 50  HFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRI-----SGKVI 109

Query: 159 DGPALR--PNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDITWLQCLPCSN 218
                R   N   + +VSG  QGSGEYF RI VG P R  Y+V D+GSD+ W+QC PC  
Sbjct: 110 PSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-- 169

Query: 219 GNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDGSFTTGDFA 278
              CYKQSDP+FDP  S SY+ +SC S  C  ++  GC SG C Y+V YGDGS+T G  A
Sbjct: 170 -KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLA 229

Query: 279 TETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLK---ASSFSYCLV 338
            ETLTF  +  V N+ +GCGH N G+F+GAAGL+G+GGG +S   QL      +F YCLV
Sbjct: 230 LETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV 289

Query: 339 DRDSDSSSTLEF-NTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTRFEID 398
            R +DS+ +L F     P  +   PL+RN R  S+ YV + G+ VGG  +P+    F++ 
Sbjct: 290 SRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLT 349

Query: 399 GSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQSNVEV 458
            +G GG+++D+GT +TRLP+  YV+ R+ F     NLP ASG+S FDTCY+L+G  +V V
Sbjct: 350 ETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRV 409

Query: 459 PTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSYDLVN 518
           PTV+F  ++G  L LPA N+L+ +D +GTYC AF  + + LSIIG+ QQ+GI+VS+D  N
Sbjct: 410 PTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGAN 469

Query: 519 SLVGFSSKKC 523
             VGF    C
Sbjct: 470 GFVGFGPNVC 470

BLAST of Tan0012058.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.5e-97
Identity = 220/485 (45.36%), Postives = 289/485 (59.59%), Query Frame = 0

Query: 52  FSLFHSSLSRPLTESTTIFDV---SASSKRAQNALSINPRSFSDS---DPVQIHSLQSPN 111
           FSL    LS P   S   F     ++ S    + +S  P S S+S      +  S    +
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSESS 69

Query: 112 SSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALNGGQKLDGPALRP 171
           SS+TL L    ++  +  K  + L  +RL RD+ RV+S+   L   + G      P  RP
Sbjct: 70  SSITLNLDHIDALSSN--KTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRNVTHAP--RP 129

Query: 172 NFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDITWLQCLPCSNGNACYKQSD 231
              ++ VVSG SQGSGEYF R+ VG PAR  Y+V DTGSDI WLQC PC     CY QSD
Sbjct: 130 GGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSD 189

Query: 232 PIFDPKSSSSYSPLSCASQQCQSLDKPGCQS--GTCVYQVSYGDGSFTTGDFATETLTFG 291
           PIFDP+ S +Y+ + C+S  C+ LD  GC +   TC+YQVSYGDGSFT GDF+TETLTF 
Sbjct: 190 PIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF- 249

Query: 292 NSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLK---ASSFSYCLVDRDSDS- 351
             N V  + +GCGHDNEGLFVGAAGL+GLG G LS   Q        FSYCLVDR + S 
Sbjct: 250 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 309

Query: 352 -SSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLP-ISSTRFEIDGSGIG 411
            SS +  N      +  +PLL N +  ++ YV + G+SVGG  +P ++++ F++D  G G
Sbjct: 310 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 369

Query: 412 GIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQSNVEVPTVAF 471
           G+I+DSGT +TRL    Y+++R+AF   A  L  A   S FDTC++L+  + V+VPTV  
Sbjct: 370 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 429

Query: 472 ELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSYDLVNSLVGF 523
              +G  + LPA NYLI +D+ G +C AF  T   LSIIG+ QQQG RV YDL +S VGF
Sbjct: 430 HF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 484

BLAST of Tan0012058.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 8.6e-72
Identity = 159/394 (40.36%), Postives = 228/394 (57.87%), Query Frame = 0

Query: 136 RDAVRVQSLNRNLELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARS 195
           ++  + Q L R +E      Q+L+     P+ +   V +    G GEY   +S+G PA+ 
Sbjct: 52  KNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYA----GDGEYLMNLSIGTPAQP 111

Query: 196 FYLVPDTGSDITWLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQ 255
           F  + DTGSD+ W QC PC+    C+ QS PIF+P+ SSS+S L C+SQ CQ+L  P C 
Sbjct: 112 FSAIMDTGSDLIWTQCQPCTQ---CFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCS 171

Query: 256 SGTCVYQVSYGDGSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVG-AAGLIGLGG 315
           +  C Y   YGDGS T G   TETLTFG S S+PN+  GCG +N+G   G  AGL+G+G 
Sbjct: 172 NNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQGFGQGNGAGLVGMGR 231

Query: 316 GPLSLSSQLKASSFSYCLVDRDSDSSSTLEFNTGRPSDSLTSP---LLRNNRFQSYRYVA 375
           GPLSL SQL  + FSYC+    S + S L   +   S +  SP   L+++++  ++ Y+ 
Sbjct: 232 GPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYIT 291

Query: 376 VTGMSVGGKPLPISSTRFEID-GSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLP 435
           + G+SVG   LPI  + F ++  +G GGII+DSGT +T   ++ Y S+R+ F+    NLP
Sbjct: 292 LNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI-NLP 351

Query: 436 PASGISP-FDTCYNL-AGQSNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLK 495
             +G S  FD C+   +  SN+++PT       G  L LP+ NY I   S G  CLA   
Sbjct: 352 VVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD-GGDLELPSENYFI-SPSNGLICLAMGS 411

Query: 496 TTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSKKC 523
           ++  +SI G+ QQQ + V YD  NS+V F+S +C
Sbjct: 412 SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Tan0012058.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.5e-65
Identity = 166/445 (37.30%), Postives = 231/445 (51.91%), Query Frame = 0

Query: 85  INPRSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSL 144
           + P S +    +  H  + P   L + L    S  G     YE L++  + R   R++S+
Sbjct: 19  VAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDS--GKNLTKYE-LIKRAIKRGERRMRSI 78

Query: 145 NRNLELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGS 204
           N  L+ +              + +  PV +    G GEY   +++G P  SF  + DTGS
Sbjct: 79  NAMLQSS--------------SGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGS 138

Query: 205 DITWLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVS 264
           D+ W QC PC+    C+ Q  PIF+P+ SSS+S L C SQ CQ L    C +  C Y   
Sbjct: 139 DLIWTQCEPCTQ---CFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYG 198

Query: 265 YGDGSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVG-AAGLIGLGGGPLSLSSQL 324
           YGDGS T G  ATET TF  ++SVPN+  GCG DN+G   G  AGLIG+G GPLSL SQL
Sbjct: 199 YGDGSTTQGYMATETFTF-ETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQL 258

Query: 325 KASSFSYCLVDRDSDSSSTLEFN---TGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGK 384
               FSYC+    S S STL      +G P  S ++ L+ ++   +Y Y+ + G++VGG 
Sbjct: 259 GVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGD 318

Query: 385 PLPISSTRFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPA-SGISPFD 444
            L I S+ F++   G GG+I+DSGT +T LP D Y ++ +AF     NLP      S   
Sbjct: 319 NLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI-NLPTVDESSSGLS 378

Query: 445 TCYNLAGQ-SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTS-SLSIIG 504
           TC+      S V+VP ++ +   G  L L   N LI   + G  CLA   ++   +SI G
Sbjct: 379 TCFQQPSDGSTVQVPEISMQFD-GGVLNLGEQNILI-SPAEGVICLAMGSSSQLGISIFG 435

Query: 505 SFQQQGIRVSYDLVNSLVGFSSKKC 523
           + QQQ  +V YDL N  V F   +C
Sbjct: 439 NIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Tan0012058.1 vs. NCBI nr
Match: XP_023531915.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.0 bits (1948), Expect = 4.4e-214
Identity = 392/495 (79.19%), Postives = 427/495 (86.26%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           MA+++N N N  IFSTFL + I N  LF SSL+R  T++T +FDVSASS RAQNALSI P
Sbjct: 1   MAISLNTNNNKPIFSTFLRIAILNSLLFSSSLARVFTQTTAVFDVSASSNRAQNALSITP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS +  NSSL+LPLH RL+IH H YKDY+SLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHRLSNSSLSLPLHSRLAIHKHNYKDYDSLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L   A+RPN LTAPVVSGQSQGSGEYFARI+VGQPA+SFYLVPDTGSDIT
Sbjct: 121 LNLA------LARDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY+Q+DPIF+P SSSSY PLSC SQQCQSLD+PGCQSGTC YQV YGD
Sbjct: 181 WLQCLPCSYGNTCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLDRPGCQSGTCAYQVRYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFTTGDFATETLTFGNS S+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTTGDFATETLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++ RPSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  AS ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGAQSLTAASAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVS 507
           +NV+VPTVAFELSKG  L+LPA NYLIRMD+AGTYCLAFLKTTSSLSIIGSFQQQG+RVS
Sbjct: 421 ANVQVPTVAFELSKGKLLQLPARNYLIRMDTAGTYCLAFLKTTSSLSIIGSFQQQGMRVS 480

Query: 508 YDLVNSLVGFSSKKC 523
           YDLVNSLVGFSS KC
Sbjct: 481 YDLVNSLVGFSSNKC 481

BLAST of Tan0012058.1 vs. NCBI nr
Match: KAG7021604.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 754.6 bits (1947), Expect = 5.7e-214
Identity = 390/495 (78.79%), Postives = 427/495 (86.26%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           M+L++NMN N  +FST LF+ I N  LF SSL+R   E+TT+FDVSASS RAQNALSI P
Sbjct: 1   MSLSLNMNNNKPLFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQNALSITP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS +  NSSL+LPLH RL+IH H+YKDY+SLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHRLSNSSLSLPLHSRLAIHKHSYKDYQSLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L G A+RPN LTAPVVSGQSQGSGEYFARI+VGQP +SFYLVPDTGSDIT
Sbjct: 121 LNLA------LAGDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY+Q+DPIF+P SSSSY PLSC SQQCQSL++PGCQSGTCVYQV YGD
Sbjct: 181 WLQCLPCSYGNTCYQQTDPIFNPTSSSSYKPLSCDSQQCQSLNRPGCQSGTCVYQVRYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFT GDF TETLTFGNS S+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTVGDFVTETLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++  PSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  A  ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVS 507
           SNV+VPTVAFELSKG  L+LPANNYLIRMD+AG+YCLAFLKTTSSLSIIGSFQQQG+RVS
Sbjct: 421 SNVQVPTVAFELSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVS 480

Query: 508 YDLVNSLVGFSSKKC 523
           YDLVNSLVGFSS KC
Sbjct: 481 YDLVNSLVGFSSNKC 481

BLAST of Tan0012058.1 vs. NCBI nr
Match: XP_023007215.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 753.1 bits (1943), Expect = 1.7e-213
Identity = 395/496 (79.64%), Postives = 426/496 (85.89%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           M +++NMN N  IFS FLF  I N  LF SSL+R  TE+TT+FDVSASS RAQNALSI P
Sbjct: 1   MPISLNMNNNKPIFSAFLFTAILNSLLFSSSLARVFTETTTVFDVSASSNRAQNALSITP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS    NSSL+L LH RL+IH H YKDYESLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHHLSNSSLSLSLHSRLAIHKHNYKDYESLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L G A+RPN LTAPVVSGQSQGSGEYFARI+VGQPA+SFYLVPDTGSDIT
Sbjct: 121 LNLA------LAGDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY Q+DPIF+P SSSSY PLSC SQQCQSL++PGCQSGTCVYQV YGD
Sbjct: 181 WLQCLPCSIGNTCYPQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFTTGDFATETLTFGNS S+PNLPIGCGHDN+GLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTTGDFATETLTFGNSKSIPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++ RPSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  A  ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLK-TTSSLSIIGSFQQQGIRV 507
           SNV+VPTVAFELSKGN L+LPA NYLIRMD+AGTYCLAFLK TTSSLSIIGSFQQQG+RV
Sbjct: 421 SNVQVPTVAFELSKGNLLQLPARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRV 480

Query: 508 SYDLVNSLVGFSSKKC 523
           SYDLVNSLVGFSS KC
Sbjct: 481 SYDLVNSLVGFSSNKC 482

BLAST of Tan0012058.1 vs. NCBI nr
Match: XP_022933467.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata] >KAG6587644.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 750.4 bits (1936), Expect = 1.1e-212
Identity = 388/495 (78.38%), Postives = 427/495 (86.26%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           M+L+++MN N  +FST LF+ I N  LF SSL+R   E+TT+FDVSASS RAQ+ALS+ P
Sbjct: 1   MSLSLDMNNNKPLFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQSALSMTP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS +  NSSL+LPLH RL+IH H+YKDYESLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHRLSNSSLSLPLHSRLAIHKHSYKDYESLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L G A+RPN LTAPVVSGQSQGSGEYFARI+VGQP +SFYLVPDTGSDIT
Sbjct: 121 LNLA------LAGDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY+Q+DPIF+P SSSSY PLSC SQQCQSL++PGCQSGTCVYQV YGD
Sbjct: 181 WLQCLPCSYGNTCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFT GDF TETLTFGNS S+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTVGDFVTETLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++  PSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  A  ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVS 507
           SNV+VPTVAFELSKG  L+LPANNYLIRMD+AG+YCLAFLKTTSSLSIIGSFQQQG+RVS
Sbjct: 421 SNVQVPTVAFELSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVS 480

Query: 508 YDLVNSLVGFSSKKC 523
           YDLVNSLVGFSS KC
Sbjct: 481 YDLVNSLVGFSSNKC 481

BLAST of Tan0012058.1 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 693.0 bits (1787), Expect = 2.0e-195
Identity = 367/494 (74.29%), Postives = 406/494 (82.19%), Query Frame = 0

Query: 36  MNSAIFSTFLFLTIFNFSLFHSSLSRPLTE---STTIFDVSASSKRAQNALSINPRSFSD 95
           MN +I S F+FLTI     F S  SR LT+   ST IFDVSAS+K+AQNALSI P+    
Sbjct: 1   MNISISSAFIFLTILTSLQFPSIFSRKLTQSPYSTAIFDVSASTKQAQNALSIKPK---- 60

Query: 96  SDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELAL 155
             P + HS   PNS L+LPLHPRL+++  +YKDY SLVRARLARDAVRVQSLNRNLEL+L
Sbjct: 61  --PFETHS-HHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSL 120

Query: 156 NGGQKLD---GPALRPNFLTAPVVSGQSQGS-GEYFARISVGQPARSFYLVPDTGSDITW 215
           NGGQK+      +   + +TAPVVSGQS G+ GEYFARI VGQP +SFYLVPDTGSD+TW
Sbjct: 121 NGGQKIGAGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTW 180

Query: 216 LQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDG 275
           LQC PC+   ACYKQ DPIFDPKSSSSY+ LSC SQQCQ LDK  C S  C YQV+YGDG
Sbjct: 181 LQCQPCAAEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDG 240

Query: 276 SFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASSF 335
           SFTTG+ ATETL+FGNSNS+PNLPIGCGHDNEGLFVGAAGLIGLGGG +SLSSQLKASSF
Sbjct: 241 SFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSF 300

Query: 336 SYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTR 395
           SYCLVD DSDSSSTLEFNT RPSDSLTSPL++N+RF SYRYV V GMSVGG PLPISSTR
Sbjct: 301 SYCLVDLDSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTR 360

Query: 396 FEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQS 455
           FEID SG+GGIIVDSGT IT+LPSDVY SLREAF+K   NLPPA GIS FDTCY+L+GQS
Sbjct: 361 FEIDESGLGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQS 420

Query: 456 NVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSY 515
           +VEVP +AF L   NSLRLPA NYLI +DS GTYCLAF KT SSLSIIGSFQQQGIRVSY
Sbjct: 421 SVEVPVIAFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSY 480

Query: 516 DLVNSLVGFSSKKC 523
           DL NSLVGFS+ KC
Sbjct: 481 DLANSLVGFSTNKC 487

BLAST of Tan0012058.1 vs. ExPASy TrEMBL
Match: A0A6J1L2D2 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499769 PE=4 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 8.0e-214
Identity = 395/496 (79.64%), Postives = 426/496 (85.89%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           M +++NMN N  IFS FLF  I N  LF SSL+R  TE+TT+FDVSASS RAQNALSI P
Sbjct: 1   MPISLNMNNNKPIFSAFLFTAILNSLLFSSSLARVFTETTTVFDVSASSNRAQNALSITP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS    NSSL+L LH RL+IH H YKDYESLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHHLSNSSLSLSLHSRLAIHKHNYKDYESLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L G A+RPN LTAPVVSGQSQGSGEYFARI+VGQPA+SFYLVPDTGSDIT
Sbjct: 121 LNLA------LAGDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY Q+DPIF+P SSSSY PLSC SQQCQSL++PGCQSGTCVYQV YGD
Sbjct: 181 WLQCLPCSIGNTCYPQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFTTGDFATETLTFGNS S+PNLPIGCGHDN+GLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTTGDFATETLTFGNSKSIPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++ RPSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  A  ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLK-TTSSLSIIGSFQQQGIRV 507
           SNV+VPTVAFELSKGN L+LPA NYLIRMD+AGTYCLAFLK TTSSLSIIGSFQQQG+RV
Sbjct: 421 SNVQVPTVAFELSKGNLLQLPARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRV 480

Query: 508 SYDLVNSLVGFSSKKC 523
           SYDLVNSLVGFSS KC
Sbjct: 481 SYDLVNSLVGFSSNKC 482

BLAST of Tan0012058.1 vs. ExPASy TrEMBL
Match: A0A6J1EZU8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111440870 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 5.2e-213
Identity = 388/495 (78.38%), Postives = 427/495 (86.26%), Query Frame = 0

Query: 28  MALNVNMNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP 87
           M+L+++MN N  +FST LF+ I N  LF SSL+R   E+TT+FDVSASS RAQ+ALS+ P
Sbjct: 1   MSLSLDMNNNKPLFSTLLFIAILNSLLFSSSLARVFKETTTVFDVSASSNRAQSALSMTP 60

Query: 88  RSFSDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRN 147
             F        HS +  NSSL+LPLH RL+IH H+YKDYESLVRARLARDA RVQSLNRN
Sbjct: 61  PQF--------HSHRLSNSSLSLPLHSRLAIHKHSYKDYESLVRARLARDAARVQSLNRN 120

Query: 148 LELALNGGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 207
           L LA      L G A+RPN LTAPVVSGQSQGSGEYFARI+VGQP +SFYLVPDTGSDIT
Sbjct: 121 LNLA------LAGDAVRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDIT 180

Query: 208 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 267
           WLQCLPCS GN CY+Q+DPIF+P SSSSY PLSC SQQCQSL++PGCQSGTCVYQV YGD
Sbjct: 181 WLQCLPCSYGNTCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGD 240

Query: 268 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 327
           GSFT GDF TETLTFGNS S+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASS
Sbjct: 241 GSFTVGDFVTETLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASS 300

Query: 328 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 387
           FSYCLVDRDSDSSSTLEF++  PSDS+T+PLL+NNR  SYRYV VTGMSVGGK L ISST
Sbjct: 301 FSYCLVDRDSDSSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISST 360

Query: 388 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 447
           RFEIDGSG+GGIIVDSGTFITRLP+DVY SLREAF++ A +L  A  ISPFDTCYNLAGQ
Sbjct: 361 RFEIDGSGMGGIIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQ 420

Query: 448 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVS 507
           SNV+VPTVAFELSKG  L+LPANNYLIRMD+AG+YCLAFLKTTSSLSIIGSFQQQG+RVS
Sbjct: 421 SNVQVPTVAFELSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVS 480

Query: 508 YDLVNSLVGFSSKKC 523
           YDLVNSLVGFSS KC
Sbjct: 481 YDLVNSLVGFSSNKC 481

BLAST of Tan0012058.1 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.4e-194
Identity = 360/494 (72.87%), Postives = 410/494 (83.00%), Query Frame = 0

Query: 36  MNSAIFSTFLFLTIFNFSLFHSSLSRPLTE---STTIFDVSASSKRAQNALSINPRSFSD 95
           M +++ S FLFLTIF    F S LSR LT+   ST+IFDV AS+ +A NALSI P+    
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKH--- 60

Query: 96  SDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELAL 155
              +Q HS   PNSSL+LPL+PRLS+H  +YKDY+SLVRARLARDA RVQ LNRNLE +L
Sbjct: 61  ---LQTHS-HLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSL 120

Query: 156 NGGQ---KLDGPALRPNFLTAPVVSGQSQGSG-EYFARISVGQPARSFYLVPDTGSDITW 215
           NGG+   ++   +L  + +TAPVVSGQS+GSG EY A++ VGQP + FYLVPDTGSD+TW
Sbjct: 121 NGGKDFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTW 180

Query: 216 LQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDG 275
           LQC PC+  NACYKQ DPIFDPKSSSSY+PLSC SQQC  LD+P C SGTC+YQV YGDG
Sbjct: 181 LQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDG 240

Query: 276 SFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASSF 335
           SFTTG+ ATETL+FGNSNS+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSF
Sbjct: 241 SFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSF 300

Query: 336 SYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTR 395
           SYCLV+ DSDSSSTLEFN+  PSDSLTSPL++N+RF SYRYV V G+SVGGK LPISSTR
Sbjct: 301 SYCLVNMDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTR 360

Query: 396 FEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQS 455
           FEID SG+GGIIVDSGT I+RLPSDVY SLREAF+KL  +L PA GIS FDTCYNL+ QS
Sbjct: 361 FEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQS 420

Query: 456 NVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSY 515
           NVEVPT+AF LS G SLRLPA NYLIR+D+AGTYCLAF+KT SSLSIIGSFQQQGIRVSY
Sbjct: 421 NVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSY 480

Query: 516 DLVNSLVGFSSKKC 523
           DL NSLVGFS+ KC
Sbjct: 481 DLTNSLVGFSTNKC 487

BLAST of Tan0012058.1 vs. ExPASy TrEMBL
Match: A0A5D3DYG2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00370 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 4.1e-194
Identity = 359/494 (72.67%), Postives = 409/494 (82.79%), Query Frame = 0

Query: 36  MNSAIFSTFLFLTIFNFSLFHSSLSRPLTE---STTIFDVSASSKRAQNALSINPRSFSD 95
           M +++ S FLFLTIF    F S LSR LT+   ST+IFDV AS+ +A NALSI P+    
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKH--- 60

Query: 96  SDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELAL 155
              +Q HS   PNSSL+LPL+PRLS+H  +YKDY+SLVRARLARDA RVQ LNRNLE +L
Sbjct: 61  ---LQTHS-HLPNSSLSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSL 120

Query: 156 NGGQ---KLDGPALRPNFLTAPVVSGQSQGSG-EYFARISVGQPARSFYLVPDTGSDITW 215
           NGG+   ++   +L  + +TAPVVSGQS+GSG EY A++ VGQP + FYLVPDTGSD+TW
Sbjct: 121 NGGKDFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTW 180

Query: 216 LQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDG 275
           LQC PC+  NACYKQ DPIFDPKSSSSY+PLSC SQQC  LD+P C SGTC+YQV YGDG
Sbjct: 181 LQCQPCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDG 240

Query: 276 SFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASSF 335
           SFTTG+ ATE L+FGNSNS+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSF
Sbjct: 241 SFTTGELATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSF 300

Query: 336 SYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTR 395
           SYCLV+ DSDSSSTLEFN+  PSDSLTSPL++N+RF SYRYV V G+SVGG  LPISSTR
Sbjct: 301 SYCLVNMDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTR 360

Query: 396 FEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQS 455
           FEID SG+GGIIVDSGT I+RLPSDVY SLREAF+KL  +L PA GIS FDTCYNL+GQS
Sbjct: 361 FEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQS 420

Query: 456 NVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSY 515
           NVEVPT+AF LS G SLRLPA NYLIR+D+AGTYCLAF+KT SSLSIIGSFQQQGIRVSY
Sbjct: 421 NVEVPTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSY 480

Query: 516 DLVNSLVGFSSKKC 523
           DL NSLVGFS+ KC
Sbjct: 481 DLTNSLVGFSTNKC 487

BLAST of Tan0012058.1 vs. ExPASy TrEMBL
Match: A0A0A0LPJ3 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=4 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.5e-191
Identity = 355/494 (71.86%), Postives = 403/494 (81.58%), Query Frame = 0

Query: 36  MNSAIFSTFLFLTIFNFSLFHSSLSRPLTE---STTIFDVSASSKRAQNALSINPRSFSD 95
           MN+++ S FLFLTIF    F S LSR LT    ST+IFDVSAS+ +A +ALSI P+    
Sbjct: 1   MNTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPK---- 60

Query: 96  SDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELAL 155
             P+Q HS   PNS  +LPL+PRL++H  +YKDY +LVRARL RDA RVQ LNRNLE +L
Sbjct: 61  --PLQNHS-HLPNSPFSLPLYPRLALHNPSYKDYNTLVRARLTRDAARVQFLNRNLERSL 120

Query: 156 NGGQKLD---GPALRPNFLTAPVVSGQSQGSG-EYFARISVGQPARSFYLVPDTGSDITW 215
           NGG         +L  + +TAPVVSGQS+GSG EY A+I VGQP + FYLVPDTGSD+TW
Sbjct: 121 NGGTHFGESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTW 180

Query: 216 LQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDG 275
           LQC PC++ N CYKQ DPIFDPKSSSSYSPLSC SQQC+ LDK  C S TC+YQV YGDG
Sbjct: 181 LQCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDG 240

Query: 276 SFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASSF 335
           SFTTG+ ATETL+FGNSNS+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSF
Sbjct: 241 SFTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSF 300

Query: 336 SYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTR 395
           SYCLV+ DSDSSSTLEFN+  PSDSLTSPL++N+RF SYRYV V G+SVGGK LPIS TR
Sbjct: 301 SYCLVNLDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTR 360

Query: 396 FEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQS 455
           FEID SG+GGIIVDSGT I+RLPSDVY SLREAF+KL  +L PA GIS FDTCYN +GQS
Sbjct: 361 FEIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQS 420

Query: 456 NVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSY 515
           NVEVPT+AF LS+G SLRLPA NYLI +D+AGTYCLAF+KT SSLSIIGSFQQQGIRVSY
Sbjct: 421 NVEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSY 480

Query: 516 DLVNSLVGFSSKKC 523
           DL NSLVGFS+ KC
Sbjct: 481 DLTNSLVGFSTNKC 487

BLAST of Tan0012058.1 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 484.6 bits (1246), Expect = 1.0e-136
Identity = 256/495 (51.72%), Postives = 341/495 (68.89%), Query Frame = 0

Query: 34  MNMNSAIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINPRSFSDS 93
           M+ N + F    FLT  + S+F   L    T +T+I +V+ S  R +   S         
Sbjct: 1   MSPNYSFFFFIFFLTSHS-SVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQ---- 60

Query: 94  DPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALN 153
              Q     S +SS +L LH R+S+ G  + DY+SL  ARL RD  RV+SL   L+LA+N
Sbjct: 61  ---QEEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAIN 120

Query: 154 GGQKLDGPALRPNFLT------APVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDIT 213
              K D   +   + T      AP++SG +QGSGEYF R+ +G+PAR  Y+V DTGSD+ 
Sbjct: 121 NISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVN 180

Query: 214 WLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGD 273
           WLQC PC++   CY Q++PIF+P SSSSY PLSC + QC +L+   C++ TC+Y+VSYGD
Sbjct: 181 WLQCTPCAD---CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGD 240

Query: 274 GSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLKASS 333
           GS+T GDFATETLT G S  V N+ +GCGH NEGLFVGAAGL+GLGGG L+L SQL  +S
Sbjct: 241 GSYTVGDFATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTS 300

Query: 334 FSYCLVDRDSDSSSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISST 393
           FSYCLVDRDSDS+ST++F T    D++ +PLLRN++  ++ Y+ +TG+SVGG+ L I  +
Sbjct: 301 FSYCLVDRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQS 360

Query: 394 RFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQ 453
            FE+D SG GGII+DSGT +TRL +++Y SLR++F+K   +L  A+G++ FDTCYNL+ +
Sbjct: 361 SFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAK 420

Query: 454 SNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVS 513
           + VEVPTVAF    G  L LPA NY+I +DS GT+CLAF  T SSL+IIG+ QQQG RV+
Sbjct: 421 TTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVT 480

Query: 514 YDLVNSLVGFSSKKC 523
           +DL NSL+GFSS KC
Sbjct: 481 FDLANSLIGFSSNKC 483

BLAST of Tan0012058.1 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 456.8 bits (1174), Expect = 2.3e-128
Identity = 250/504 (49.60%), Postives = 338/504 (67.06%), Query Frame = 0

Query: 39  AIFSTFLFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINP--------RSF 98
           A+ +  LFLT  + S    SLS P    T + DV +S ++ Q  LS++P        +  
Sbjct: 11  AVVTLSLFLTTTDAS--SRSLSTP--PKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 70

Query: 99  SDSDPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLEL 158
           S SDPV  +S    +S L+L LH R +     +KDY+SL  +RL RD+ RV  +   +  
Sbjct: 71  SLSDPVFFNS----SSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRF 130

Query: 159 ALNGGQKLDGPALRPNF----------LTAPVVSGQSQGSGEYFARISVGQPARSFYLVP 218
           A+ G  + D   L+P +          LT PVVSG SQGSGEYF+RI VG PA+  YLV 
Sbjct: 131 AVEGVDRSD---LKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVL 190

Query: 219 DTGSDITWLQCLPCSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCV 278
           DTGSD+ W+QC PC++   CY+QSDP+F+P SSS+Y  L+C++ QC  L+   C+S  C+
Sbjct: 191 DTGSDVNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCL 250

Query: 279 YQVSYGDGSFTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLS 338
           YQVSYGDGSFT G+ AT+T+TFGNS  + N+ +GCGHDNEGLF GAAGL+GLGGG LS++
Sbjct: 251 YQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSIT 310

Query: 339 SQLKASSFSYCLVDRDSDSSSTLEFNTGR-PSDSLTSPLLRNNRFQSYRYVAVTGMSVGG 398
           +Q+KA+SFSYCLVDRDS  SS+L+FN+ +      T+PLLRN +  ++ YV ++G SVGG
Sbjct: 311 NQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGG 370

Query: 399 KPLPISSTRFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPP-ASGISPF 458
           + + +    F++D SG GG+I+D GT +TRL +  Y SLR+AF+KL  NL   +S IS F
Sbjct: 371 EKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLF 430

Query: 459 DTCYNLAGQSNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGS 518
           DTCY+ +  S V+VPTVAF  + G SL LPA NYLI +D +GT+C AF  T+SSLSIIG+
Sbjct: 431 DTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGN 490

Query: 519 FQQQGIRVSYDLVNSLVGFSSKKC 523
            QQQG R++YDL  +++G S  KC
Sbjct: 491 VQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Tan0012058.1 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 365.5 bits (937), Expect = 6.9e-101
Identity = 222/503 (44.14%), Postives = 300/503 (59.64%), Query Frame = 0

Query: 36  MNSAIFSTF--LFLTIFNFSLFHSSLSRPLTESTTIFDVSASSKRAQNALSINPRSFSDS 95
           +N+  FS F  LF T    S + + +   L  S T+                   S+ +S
Sbjct: 6   LNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATL-------------------SWPES 65

Query: 96  DPVQIHSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALN 155
           + +   SL    +SL++ L    ++   +      L   RL RD++RV+S+     ++  
Sbjct: 66  ESLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTG 125

Query: 156 GGQKLDGPALRPNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDITWLQCLP 215
                  P     F +  V+SG SQGSGEYF R+ VG PA + Y+V DTGSD+ WLQC P
Sbjct: 126 RNATKRTPRTAGGF-SGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSP 185

Query: 216 CSNGNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPG-C---QSGTCVYQVSYGDGS 275
           C    ACY Q+D IFDPK S +++ + C S+ C+ LD    C   +S TC+YQVSYGDGS
Sbjct: 186 C---KACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGS 245

Query: 276 FTTGDFATETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLK---AS 335
           FT GDF+TETLTF  +  V ++P+GCGHDNEGLFVGAAGL+GLG G LS  SQ K     
Sbjct: 246 FTEGDFSTETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNG 305

Query: 336 SFSYCLVDRDSDSS-----STLEF-NTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGK 395
            FSYCLVDR S  S     ST+ F N   P  S+ +PLL N +  ++ Y+ + G+SVGG 
Sbjct: 306 KFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGS 365

Query: 396 PLP-ISSTRFEIDGSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFD 455
            +P +S ++F++D +G GG+I+DSGT +TRL    YV+LR+AF   A  L  A   S FD
Sbjct: 366 RVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFD 425

Query: 456 TCYNLAGQSNVEVPTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSF 515
           TC++L+G + V+VPTV F    G  + LPA+NYLI +++ G +C AF  T  SLSIIG+ 
Sbjct: 426 TCFDLSGMTTVKVPTVVFHFG-GGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNI 483

Query: 516 QQQGIRVSYDLVNSLVGFSSKKC 523
           QQQG RV+YDLV S VGF S+ C
Sbjct: 486 QQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of Tan0012058.1 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-100
Identity = 192/430 (44.65%), Postives = 263/430 (61.16%), Query Frame = 0

Query: 99  HSLQSPNSSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALNGGQKL 158
           H     +S  TL L  R       Y+++   + AR+ RD  RV ++ R +      G+ +
Sbjct: 50  HFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRI-----SGKVI 109

Query: 159 DGPALR--PNFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDITWLQCLPCSN 218
                R   N   + +VSG  QGSGEYF RI VG P R  Y+V D+GSD+ W+QC PC  
Sbjct: 110 PSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC-- 169

Query: 219 GNACYKQSDPIFDPKSSSSYSPLSCASQQCQSLDKPGCQSGTCVYQVSYGDGSFTTGDFA 278
              CYKQSDP+FDP  S SY+ +SC S  C  ++  GC SG C Y+V YGDGS+T G  A
Sbjct: 170 -KLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLA 229

Query: 279 TETLTFGNSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLK---ASSFSYCLV 338
            ETLTF  +  V N+ +GCGH N G+F+GAAGL+G+GGG +S   QL      +F YCLV
Sbjct: 230 LETLTFAKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV 289

Query: 339 DRDSDSSSTLEF-NTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLPISSTRFEID 398
            R +DS+ +L F     P  +   PL+RN R  S+ YV + G+ VGG  +P+    F++ 
Sbjct: 290 SRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLT 349

Query: 399 GSGIGGIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQSNVEV 458
            +G GG+++D+GT +TRLP+  YV+ R+ F     NLP ASG+S FDTCY+L+G  +V V
Sbjct: 350 ETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRV 409

Query: 459 PTVAFELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSYDLVN 518
           PTV+F  ++G  L LPA N+L+ +D +GTYC AF  + + LSIIG+ QQ+GI+VS+D  N
Sbjct: 410 PTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGAN 469

Query: 519 SLVGFSSKKC 523
             VGF    C
Sbjct: 470 GFVGFGPNVC 470

BLAST of Tan0012058.1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.5e-98
Identity = 220/485 (45.36%), Postives = 289/485 (59.59%), Query Frame = 0

Query: 52  FSLFHSSLSRPLTESTTIFDV---SASSKRAQNALSINPRSFSDS---DPVQIHSLQSPN 111
           FSL    LS P   S   F     ++ S    + +S  P S S+S      +  S    +
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSESS 69

Query: 112 SSLTLPLHPRLSIHGHAYKDYESLVRARLARDAVRVQSLNRNLELALNGGQKLDGPALRP 171
           SS+TL L    ++  +  K  + L  +RL RD+ RV+S+   L   + G      P  RP
Sbjct: 70  SSITLNLDHIDALSSN--KTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRNVTHAP--RP 129

Query: 172 NFLTAPVVSGQSQGSGEYFARISVGQPARSFYLVPDTGSDITWLQCLPCSNGNACYKQSD 231
              ++ VVSG SQGSGEYF R+ VG PAR  Y+V DTGSDI WLQC PC     CY QSD
Sbjct: 130 GGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSD 189

Query: 232 PIFDPKSSSSYSPLSCASQQCQSLDKPGCQS--GTCVYQVSYGDGSFTTGDFATETLTFG 291
           PIFDP+ S +Y+ + C+S  C+ LD  GC +   TC+YQVSYGDGSFT GDF+TETLTF 
Sbjct: 190 PIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF- 249

Query: 292 NSNSVPNLPIGCGHDNEGLFVGAAGLIGLGGGPLSLSSQLK---ASSFSYCLVDRDSDS- 351
             N V  + +GCGHDNEGLFVGAAGL+GLG G LS   Q        FSYCLVDR + S 
Sbjct: 250 RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSK 309

Query: 352 -SSTLEFNTGRPSDSLTSPLLRNNRFQSYRYVAVTGMSVGGKPLP-ISSTRFEIDGSGIG 411
            SS +  N      +  +PLL N +  ++ YV + G+SVGG  +P ++++ F++D  G G
Sbjct: 310 PSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNG 369

Query: 412 GIIVDSGTFITRLPSDVYVSLREAFMKLAGNLPPASGISPFDTCYNLAGQSNVEVPTVAF 471
           G+I+DSGT +TRL    Y+++R+AF   A  L  A   S FDTC++L+  + V+VPTV  
Sbjct: 370 GVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVL 429

Query: 472 ELSKGNSLRLPANNYLIRMDSAGTYCLAFLKTTSSLSIIGSFQQQGIRVSYDLVNSLVGF 523
              +G  + LPA NYLI +D+ G +C AF  T   LSIIG+ QQQG RV YDL +S VGF
Sbjct: 430 HF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 484