Tan0012034.3 (mRNA) Snake gourd v1

BLAST of Tan0012034.3 vs. ExPASy Swiss-Prot
Match: Q8VYC8 (E3 ubiquitin protein ligase RIN2 OS=Arabidopsis thaliana OX=3702 GN=RIN2 PE=1 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.8e-209
Identity = 375/577 (64.99%), Postives = 455/577 (78.86%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG+ Y+  S A T LSF+GLQ+WTELS+D+LR++ +I +N     ++   ++LLL SY T
Sbjct: 1   MGIKYLPVSVASTALSFVGLQVWTELSLDRLRADGLIAKN-ISLGDSEHALELLLGSYFT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LL NF LNV++LL+L LKT+FF +LY  E++KLVERL NY+IYKGTFLPLVIPPT + 
Sbjct: 61  IALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANYIIYKGTFLPLVIPPTIFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW++WL VLC+LKMFQALARDRLERLNASPS+T WTYFRVYSVL LVL VD+  I+  
Sbjct: 121 GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTYFRVYSVLFLVLSVDMFWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LM Y T+G +++LLLLFEP SIAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF + 
Sbjct: 181 LMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFIDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHG+ F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGIAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRI+ ++KL  ALGALHAALPDAT+EEL+AY+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 AILKRIKGYIKLRIALGALHAALPDATSEELRAYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR EN+VN R  E  SDEQLARQ    L RQN  +
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTENEVNPRTVEVSSDEQLARQ----LERQNNPV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
             L  G+FP +  + VE    R+  LDPSWL  W+SQG D AGPS  +R+VGLGRVQMMM
Sbjct: 421 HALATGLFPAEVPDSVENDTSRNLGLDPSWLQTWSSQGSDVAGPSTTSRTVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGSQVPRSVVGRHPGSMGGLNM-NVPRAS 540
           RHLASVGE+YAQT+++D+AW LWP+NPSQ +  S    +V   + G  GGL++  V   +
Sbjct: 481 RHLASVGESYAQTALDDAAWSLWPMNPSQASTSS---TTVPPGNGGRTGGLHLRTVSNTT 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSSCA 577
           N++L NILAMAETVREV+PHVPDE+IFQ     +S A
Sbjct: 541 NESLTNILAMAETVREVMPHVPDEIIFQDLQRTNSVA 569

BLAST of Tan0012034.3 vs. ExPASy Swiss-Prot
Match: Q8W4Q5 (E3 ubiquitin protein ligase RIN3 OS=Arabidopsis thaliana OX=3702 GN=RIN3 PE=1 SV=2)

HSP 1 Score: 716.1 bits (1847), Expect = 3.5e-205
Identity = 365/575 (63.48%), Postives = 450/575 (78.26%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG++Y+  S A T LSF+GLQ+WTELS+D+LR++ II +N     N+   ++LLLSS+ T
Sbjct: 1   MGITYLHISVATTALSFVGLQVWTELSLDRLRADGIITKN-ISLGNSENTLELLLSSHTT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LLA+F LN+++LL+L LKT+FF +LY+ E+RKLVERL NY+IYKGTFLP V+P T + 
Sbjct: 61  IALLASFVLNIYILLVLSLKTLFFGDLYAIETRKLVERLANYIIYKGTFLPFVVPRTVFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW+IWL VLC+LKMFQALARDRL+RLNASPS+T WTYFRVYS L +VL  DL  I+  
Sbjct: 121 GVLWTIWLTVLCTLKMFQALARDRLDRLNASPSSTPWTYFRVYSALFMVLSTDLCWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIY T+G S++LLLLFEP  IAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF++ 
Sbjct: 181 LMIYNTVGSSVYLLLLFEPCGIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFYDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHGM F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGMAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           +I+KRI+ ++KL  ALGALHAAL DAT+EEL+ Y+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 SILKRIKGYIKLRVALGALHAALLDATSEELRDYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR E++ N   GE  SDE LARQ      RQN ++
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTESEANPSRGEVSSDEHLARQ----FERQNNSV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
             L  G+FP +T N  E  PWR++ +DPSWL  W+ QGVD  G S  +RSVGLG+VQ+MM
Sbjct: 421 HALTTGMFPTETPNFTESDPWRNSEVDPSWLQTWSDQGVDVVGSSAGSRSVGLGQVQLMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGS-QVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGE  AQT+++D++WGLWP+NPSQ +  S  VP    GR     GGL++     +
Sbjct: 481 RHLASVGEGSAQTTLDDASWGLWPMNPSQASTSSTYVPPGAGGR----TGGLHLRTVSRA 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
            +N+A+ILAMAETVREVLPHVPDE+IFQ     +S
Sbjct: 541 ANNMASILAMAETVREVLPHVPDEIIFQDLQRTNS 566

BLAST of Tan0012034.3 vs. ExPASy Swiss-Prot
Match: Q9R049 (E3 ubiquitin-protein ligase AMFR OS=Mus musculus OX=10090 GN=Amfr PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 6.0e-32
Identity = 108/369 (29.27%), Postives = 168/369 (45.53%), Query Frame = 0

Query: 50  VVDLLLSSYITVGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTF 109
           V   LLS  + V +L N A  V +L+   ++ I F  L  SE + L ++  N++ YK  F
Sbjct: 73  VAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIF 132

Query: 110 LPLVI-PPTKYHAALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLL 169
           +  V+   T     +W +W A L  L +   L +DR E L+ SP+    ++ RV S+L+ 
Sbjct: 133 IFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLIA 192

Query: 170 VLFVDLSGIRFCLMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGN 229
           +L         C +   T G+     +  E L +   T   IL +     D  L+H    
Sbjct: 193 MLLSCCGLAVVCCVTGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWD--LNHE--- 252

Query: 230 CSNCQISKFFNMTIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVD 289
                             WE KG+ +    F +++A L + L H++H+     +  ++  
Sbjct: 253 ----------------GTWEGKGTYVYYTDFVMELALLSLDLMHHIHMLLFGNIWLSMAS 312

Query: 290 AVLFLNIRALLSAIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKA 349
            V+F+ +R L   + +RIR     ++ +G + A    AT EEL   ND+CAIC + M  A
Sbjct: 313 LVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAA 372

Query: 350 KKLNCNHLFHLACLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQ 409
           +KL C HLFH +CLRSWL+Q      SCPTCR  L +           GE   +  +   
Sbjct: 373 RKLPCGHLFHNSCLRSWLEQDT----SCPTCRMSLNIADGSRAREDHQGENLDENLVPVA 416

Query: 410 ISAGLNRQN 418
            + G  R N
Sbjct: 433 AAEGRPRLN 416

BLAST of Tan0012034.3 vs. ExPASy Swiss-Prot
Match: Q9UKV5 (E3 ubiquitin-protein ligase AMFR OS=Homo sapiens OX=9606 GN=AMFR PE=1 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 1.0e-31
Identity = 107/369 (29.00%), Postives = 167/369 (45.26%), Query Frame = 0

Query: 50  VVDLLLSSYITVGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTF 109
           V   LLS  + V +L N A  V +L+   ++ I F  L  SE + L ++  N++ YK  F
Sbjct: 73  VAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIF 132

Query: 110 LPLVI-PPTKYHAALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLL 169
           +  V+   T     +W +W A L  L +   L +DR E L+ SP+    ++ RV S+L+ 
Sbjct: 133 IFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVA 192

Query: 170 VLFVDLSGIRFCLMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGN 229
           +L         C +   T G+     +  E L +   T   IL +     D  L+H    
Sbjct: 193 MLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWD--LNHE--- 252

Query: 230 CSNCQISKFFNMTIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVD 289
                             WE KG+ +    F +++  L + L H++H+     +  ++  
Sbjct: 253 ----------------GTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMAS 312

Query: 290 AVLFLNIRALLSAIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKA 349
            V+F+ +R L   + +RIR     ++ +G + A    AT EEL   ND+CAIC + M  A
Sbjct: 313 LVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAA 372

Query: 350 KKLNCNHLFHLACLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQ 409
           +KL C HLFH +CLRSWL+Q      SCPTCR  L +           GE   +  +   
Sbjct: 373 RKLPCGHLFHNSCLRSWLEQDT----SCPTCRMSLNIADNNRVREEHQGENLDENLVPVA 416

Query: 410 ISAGLNRQN 418
            + G  R N
Sbjct: 433 AAEGRPRLN 416

BLAST of Tan0012034.3 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.6e-16
Identity = 91/338 (26.92%), Postives = 152/338 (44.97%), Query Frame = 0

Query: 54  LLSSYITVGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLV 113
           L +S I   LL N  L    +    +K +F   L  +E  +L E+    V+       ++
Sbjct: 36  LSTSKICFVLLLNTGLVAMCVAWQLVKRLFLGTLREAEVERLNEQAWREVV------EIL 95

Query: 114 IPPTKYHAALWSIWLAVLCSLKMFQA---LARDRLERLNASPSATSWTYFRVYSVLLLVL 173
              T +       +LA++ +L + +A   LA+ R+E +  +PS    ++ R+ S +L +L
Sbjct: 96  FAVTIFRQDFSVSFLAMVAALLLVKALHWLAQKRVEYIETTPSVPMLSHARIVSFMLFLL 155

Query: 174 FVD---LSGIRFCLMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAG 233
            VD   LS     L+  R   +++F    FE + +A  T+   + + F   D+ +     
Sbjct: 156 VVDCLFLSNSLRSLIHKREASVAIF--FSFEYMILATSTVSTFVKYIFYVSDMLME---- 215

Query: 234 NCSNCQISKFFNMTIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLV 293
                              WE K      L    D+  L + +  ++ I+  +G+  +L+
Sbjct: 216 -----------------GQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYGVPLHLI 275

Query: 294 DAV--LFLNIRALLSAIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPM 353
             +   F N R        RI  +++  K    ++   PDAT +EL A +  C ICRE M
Sbjct: 276 RELYETFRNFRI-------RIADYVRYRKITSNMNERFPDATADELNASDATCIICREEM 333

Query: 354 AKAKKLNCNHLFHLACLRSWLDQGLNEYYSCPTCRKPL 384
             AKKL C HLFH+ CLRSWL++     ++CPTCR P+
Sbjct: 336 TTAKKLLCGHLFHVHCLRSWLER----QHTCPTCRAPI 333

BLAST of Tan0012034.3 vs. NCBI nr
Match: XP_022957821.1 (E3 ubiquitin protein ligase RIN2-like [Cucurbita moschata] >XP_022957822.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita moschata] >XP_022957823.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita moschata])

HSP 1 Score: 1070.1 bits (2766), Expect = 7.1e-309
Identity = 543/575 (94.43%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIAFSAACTVLSFIGLQLWTELSID LRSEKIIGENPFQTENAGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDNLRSEKIIGENPFQTENAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLL+LFLKTIFFVELYSSESRKLVERLVNY+IYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLMLFLKTIFFVELYSSESRKLVERLVNYIIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGL LFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSA NCSNCQISKFFNM
Sbjct: 181 LMIYRTLGLPLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAENCSNCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFL+IRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLHIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AIIKRIR FMKLMKALGALHAALPDAT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIIKRIRVFMKLMKALGALHAALPDATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN  AGETF+DEQLARQISAG+NRQN+ I
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPHAGETFNDEQLARQISAGINRQNSAI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARS GLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNTVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSAGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIE+SAWGLWPLNPSQ VAGGSQVPRSVVGRHPGS GGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEESAWGLWPLNPSQVVAGGSQVPRSVVGRHPGSTGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. NCBI nr
Match: XP_023533476.1 (E3 ubiquitin protein ligase RIN2-like [Cucurbita pepo subsp. pepo] >XP_023533477.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita pepo subsp. pepo] >XP_023533478.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069.7 bits (2765), Expect = 9.5e-309
Identity = 542/575 (94.26%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIAFSAACTVLSFIGLQLWTELSID LRSEK+IGENPFQTENAGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDNLRSEKVIGENPFQTENAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLL+LFLKTIFFVELYSSESRKLVERLVNY+IYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLMLFLKTIFFVELYSSESRKLVERLVNYIIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGL LFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSA NCSNCQISKFFNM
Sbjct: 181 LMIYRTLGLPLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAENCSNCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFL+IRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLHIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AIIKRIR FMKLMKALGALHAALPDAT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIIKRIRVFMKLMKALGALHAALPDATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN  AGETF+DEQLARQISAG+NRQN+ I
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPHAGETFNDEQLARQISAGINRQNSAI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARS GLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNTVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSAGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIE+SAWGLWPLNPSQ VAGGSQVPRSVVGRHPGS GGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEESAWGLWPLNPSQVVAGGSQVPRSVVGRHPGSTGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. NCBI nr
Match: XP_022995635.1 (E3 ubiquitin protein ligase RIN2-like [Cucurbita maxima] >XP_022995636.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita maxima] >XP_022995637.1 E3 ubiquitin protein ligase RIN2-like [Cucurbita maxima])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.4e-307
Identity = 540/575 (93.91%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIAFSAACTVLSFIGLQLWTELSID LRSEKIIGENPFQTENAGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDNLRSEKIIGENPFQTENAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLL+LFLKTIFFVELYSSESRKLVERLVNY+IYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLMLFLKTIFFVELYSSESRKLVERLVNYIIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGL LFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSA NCSNCQISKFFNM
Sbjct: 181 LMIYRTLGLPLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAENCSNCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFL+IRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLHIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AIIKRIR FMKLMKALGALHAALP+AT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIIKRIRVFMKLMKALGALHAALPNATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN  AG+TF+DEQLARQISAG+NRQNA I
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPHAGDTFNDEQLARQISAGINRQNAAI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNA+RS GLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNTVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNASRSAGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIE+SAWGLWPLNPSQ VAGGSQVPRSVVGRHPG+ GGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEESAWGLWPLNPSQVVAGGSQVPRSVVGRHPGNTGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. NCBI nr
Match: XP_038888614.1 (E3 ubiquitin protein ligase RIN2-like [Benincasa hispida])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.4e-306
Identity = 539/575 (93.74%), Postives = 553/575 (96.17%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIA SAACTVLSFIGLQLWTELSID LRSEKIIGENPF+TEN GRV+DLLLSSY+T
Sbjct: 1   MGVSYIAISAACTVLSFIGLQLWTELSIDNLRSEKIIGENPFKTENTGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGLSLFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSAGN S+CQISKFFNM
Sbjct: 181 LMIYRTLGLSLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAGNNSDCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TI G LWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS
Sbjct: 241 TIPGLLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRIR FM+L KALGALHAALPDAT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIVKRIRGFMRLRKALGALHAALPDATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQE+ VN R GETF+DEQLARQISAGLNRQN+TI
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQESHVNPRVGETFNDEQLARQISAGLNRQNSTI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARSVGLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNHVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIEDSAWGLWPLNPSQ VAGGSQ+PR+VVGRHPGSMGGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVVAGGSQIPRNVVGRHPGSMGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. NCBI nr
Match: XP_022156809.1 (E3 ubiquitin protein ligase RIN2-like [Momordica charantia])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.7e-303
Identity = 533/575 (92.70%), Postives = 549/575 (95.48%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIA SAACTVLSF+GLQLWTELSI+ L+SEKIIGENPFQTE+AGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAISAACTVLSFVGLQLWTELSIENLKSEKIIGENPFQTESAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGLSLFLLL FEPLSIAFETLQAILVH FQ LDIWLHHSAG  SNCQI++FFN 
Sbjct: 181 LMIYRTLGLSLFLLLFFEPLSIAFETLQAILVHSFQLLDIWLHHSAGYSSNCQITEFFNT 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           A IKRIR FMKL KALGALHAALPDAT+EELQ+Y+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 ATIKRIRGFMKLRKALGALHAALPDATSEELQSYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN RAG TFSDEQLARQISAGLNRQNAT+
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPRAGGTFSDEQLARQISAGLNRQNATV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARSVGLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNSVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVA-GGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIEDSAWGLWP+NP QV  GGSQVPR  VGRHPGSMGGLNMNVPRA 
Sbjct: 481 RHLASVGETYAQTSIEDSAWGLWPINPPQVVPGGSQVPRGGVGRHPGSMGGLNMNVPRAP 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           ND+LANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDSLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. ExPASy TrEMBL
Match: A0A6J1H0A8 (E3 ubiquitin protein ligase RIN2-like OS=Cucurbita moschata OX=3662 GN=LOC111459248 PE=4 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.4e-309
Identity = 543/575 (94.43%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIAFSAACTVLSFIGLQLWTELSID LRSEKIIGENPFQTENAGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDNLRSEKIIGENPFQTENAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLL+LFLKTIFFVELYSSESRKLVERLVNY+IYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLMLFLKTIFFVELYSSESRKLVERLVNYIIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGL LFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSA NCSNCQISKFFNM
Sbjct: 181 LMIYRTLGLPLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAENCSNCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFL+IRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLHIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AIIKRIR FMKLMKALGALHAALPDAT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIIKRIRVFMKLMKALGALHAALPDATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN  AGETF+DEQLARQISAG+NRQN+ I
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPHAGETFNDEQLARQISAGINRQNSAI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARS GLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNTVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSAGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIE+SAWGLWPLNPSQ VAGGSQVPRSVVGRHPGS GGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEESAWGLWPLNPSQVVAGGSQVPRSVVGRHPGSTGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. ExPASy TrEMBL
Match: A0A6J1K4H8 (E3 ubiquitin protein ligase RIN2-like OS=Cucurbita maxima OX=3661 GN=LOC111491111 PE=4 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.6e-308
Identity = 540/575 (93.91%), Postives = 555/575 (96.52%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIAFSAACTVLSFIGLQLWTELSID LRSEKIIGENPFQTENAGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDNLRSEKIIGENPFQTENAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLL+LFLKTIFFVELYSSESRKLVERLVNY+IYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLMLFLKTIFFVELYSSESRKLVERLVNYIIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGL LFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSA NCSNCQISKFFNM
Sbjct: 181 LMIYRTLGLPLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAENCSNCQISKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFL+IRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLHIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AIIKRIR FMKLMKALGALHAALP+AT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIIKRIRVFMKLMKALGALHAALPNATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN  AG+TF+DEQLARQISAG+NRQNA I
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPHAGDTFNDEQLARQISAGINRQNAAI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNA+RS GLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNTVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNASRSAGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIE+SAWGLWPLNPSQ VAGGSQVPRSVVGRHPG+ GGLNMNVPRAS
Sbjct: 481 RHLASVGETYAQTSIEESAWGLWPLNPSQVVAGGSQVPRSVVGRHPGNTGGLNMNVPRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           NDNLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDNLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. ExPASy TrEMBL
Match: A0A6J1DUP7 (E3 ubiquitin protein ligase RIN2-like OS=Momordica charantia OX=3673 GN=LOC111023642 PE=4 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.8e-303
Identity = 533/575 (92.70%), Postives = 549/575 (95.48%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIA SAACTVLSF+GLQLWTELSI+ L+SEKIIGENPFQTE+AGRV+DLLLSSY+T
Sbjct: 1   MGVSYIAISAACTVLSFVGLQLWTELSIENLKSEKIIGENPFQTESAGRVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
           AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC
Sbjct: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGLSLFLLL FEPLSIAFETLQAILVH FQ LDIWLHHSAG  SNCQI++FFN 
Sbjct: 181 LMIYRTLGLSLFLLLFFEPLSIAFETLQAILVHSFQLLDIWLHHSAGYSSNCQITEFFNT 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS
Sbjct: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           A IKRIR FMKL KALGALHAALPDAT+EELQ+Y+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 ATIKRIRGFMKLRKALGALHAALPDATSEELQSYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN RAG TFSDEQLARQISAGLNRQNAT+
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPRAGGTFSDEQLARQISAGLNRQNATV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQN VEGSPWRSTSLDPSWLHDWASQ VDGAGPSNAARSVGLGRVQMMM
Sbjct: 421 PTLPAGIFPNQTQNSVEGSPWRSTSLDPSWLHDWASQSVDGAGPSNAARSVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVA-GGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIEDSAWGLWP+NP QV  GGSQVPR  VGRHPGSMGGLNMNVPRA 
Sbjct: 481 RHLASVGETYAQTSIEDSAWGLWPINPPQVVPGGSQVPRGGVGRHPGSMGGLNMNVPRAP 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           ND+LANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NDSLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. ExPASy TrEMBL
Match: A0A1S3BMI6 (E3 ubiquitin protein ligase RIN2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491275 PE=4 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.0e-300
Identity = 531/575 (92.35%), Postives = 545/575 (94.78%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIA SAACT LSFIGLQLWTELSID LRSEKIIGENPF+TENA  VVDLLLSSY+T
Sbjct: 1   MGVSYIAISAACTALSFIGLQLWTELSIDNLRSEKIIGENPFKTENAAPVVDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLLILFLKTI FVELYSSESRK+VERLVNYVIYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLILFLKTIIFVELYSSESRKMVERLVNYVIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
            ALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYF VYSVLLLVL VDLSGIRFC
Sbjct: 121 VALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFCVYSVLLLVLIVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGLSLFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSAGN +NCQ SKFFNM
Sbjct: 181 LMIYRTLGLSLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAGNNTNCQFSKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TI G LWELKG+LIRNLGFFLDMATLLMALGHYV+IWRLHGMTFNLVDAVLFLNIRALLS
Sbjct: 241 TIPGLLWELKGTLIRNLGFFLDMATLLMALGHYVYIWRLHGMTFNLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRIR FMKL KALGAL+AALPDAT EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIVKRIRGFMKLRKALGALNAALPDATPEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN RAGETFSDEQLARQISAGLNRQNATI
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPRAGETFSDEQLARQISAGLNRQNATI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLP GIFPNQTQNPVEGSPWRSTSLDPSWLHDW SQ +DGAGPSNAARSVGLGRVQMMM
Sbjct: 421 PTLPTGIFPNQTQNPVEGSPWRSTSLDPSWLHDWTSQSMDGAGPSNAARSVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIEDSAW LWPLNPSQ VAGGSQVPR+VVGRHPGSMGGLNMNV RAS
Sbjct: 481 RHLASVGETYAQTSIEDSAWSLWPLNPSQVVAGGSQVPRNVVGRHPGSMGGLNMNVARAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           N+NLANILAMAETVREVLPHVPDELIFQ     +S
Sbjct: 541 NENLANILAMAETVREVLPHVPDELIFQDLQRTNS 575

BLAST of Tan0012034.3 vs. ExPASy TrEMBL
Match: A0A0A0KG75 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G408180 PE=4 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.9e-300
Identity = 528/568 (92.96%), Postives = 544/568 (95.77%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MGVSYIA SAACT LSFIGLQLWTELSID LRSEKIIGENPF+TENA  V+DLLLSSY+T
Sbjct: 1   MGVSYIAISAACTALSFIGLQLWTELSIDNLRSEKIIGENPFKTENAAPVIDLLLSSYVT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           VGLLANFALNVFVLLILFLKT+ FVELYSSESRK+VERLVNYVIYKGTFLPLV+PPTKYH
Sbjct: 61  VGLLANFALNVFVLLILFLKTLIFVELYSSESRKMVERLVNYVIYKGTFLPLVVPPTKYH 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
            ALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYF VYSVLLLVL VDLSGIRFC
Sbjct: 121 IALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFCVYSVLLLVLIVDLSGIRFC 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIYRTLGLSLFLLL FEPLSIAFETLQAILVHGFQ LDIWLHHSAGN +NCQ SKFFNM
Sbjct: 181 LMIYRTLGLSLFLLLFFEPLSIAFETLQAILVHGFQLLDIWLHHSAGNNTNCQFSKFFNM 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
           TI G LWELKG+LIRNLGFFLDMATLLMALGHYV+IWRLHGMTFNLVDAVLFLNIRALLS
Sbjct: 241 TIPGLLWELKGTLIRNLGFFLDMATLLMALGHYVYIWRLHGMTFNLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRIR FMKL KALGAL+AALPDAT+EELQAY+DECAICREPMAKAKKLNCNHLFHLA
Sbjct: 301 AIVKRIRGFMKLRKALGALNAALPDATSEELQAYDDECAICREPMAKAKKLNCNHLFHLA 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVN RAGETFSDEQLARQISAGLNRQNATI
Sbjct: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNPRAGETFSDEQLARQISAGLNRQNATI 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
           PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQ +DGAGPSNAARSVGL RVQMMM
Sbjct: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQSMDGAGPSNAARSVGLSRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQ-VAGGSQVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGETYAQTSIEDSAW LWPLN SQ VAGGSQVPR+VVGRHPGSMGGLNMNV RAS
Sbjct: 481 RHLASVGETYAQTSIEDSAWSLWPLNSSQVVAGGSQVPRNVVGRHPGSMGGLNMNVSRAS 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQ 568
           NDNLANI+AMAETVREVLPHVPDELIFQ
Sbjct: 541 NDNLANIIAMAETVREVLPHVPDELIFQ 568

BLAST of Tan0012034.3 vs. TAIR 10
Match: AT4G25230.1 (RPM1 interacting protein 2 )

HSP 1 Score: 730.3 bits (1884), Expect = 1.3e-210
Identity = 375/577 (64.99%), Postives = 455/577 (78.86%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG+ Y+  S A T LSF+GLQ+WTELS+D+LR++ +I +N     ++   ++LLL SY T
Sbjct: 1   MGIKYLPVSVASTALSFVGLQVWTELSLDRLRADGLIAKN-ISLGDSEHALELLLGSYFT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LL NF LNV++LL+L LKT+FF +LY  E++KLVERL NY+IYKGTFLPLVIPPT + 
Sbjct: 61  IALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANYIIYKGTFLPLVIPPTIFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW++WL VLC+LKMFQALARDRLERLNASPS+T WTYFRVYSVL LVL VD+  I+  
Sbjct: 121 GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTYFRVYSVLFLVLSVDMFWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LM Y T+G +++LLLLFEP SIAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF + 
Sbjct: 181 LMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFIDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHG+ F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGIAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRI+ ++KL  ALGALHAALPDAT+EEL+AY+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 AILKRIKGYIKLRIALGALHAALPDATSEELRAYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR EN+VN R  E  SDEQLARQ    L RQN  +
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTENEVNPRTVEVSSDEQLARQ----LERQNNPV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
             L  G+FP +  + VE    R+  LDPSWL  W+SQG D AGPS  +R+VGLGRVQMMM
Sbjct: 421 HALATGLFPAEVPDSVENDTSRNLGLDPSWLQTWSSQGSDVAGPSTTSRTVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGSQVPRSVVGRHPGSMGGLNM-NVPRAS 540
           RHLASVGE+YAQT+++D+AW LWP+NPSQ +  S    +V   + G  GGL++  V   +
Sbjct: 481 RHLASVGESYAQTALDDAAWSLWPMNPSQASTSS---TTVPPGNGGRTGGLHLRTVSNTT 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSSCA 577
           N++L NILAMAETVREV+PHVPDE+IFQ     +S A
Sbjct: 541 NESLTNILAMAETVREVMPHVPDEIIFQDLQRTNSVA 569

BLAST of Tan0012034.3 vs. TAIR 10
Match: AT4G25230.2 (RPM1 interacting protein 2 )

HSP 1 Score: 730.3 bits (1884), Expect = 1.3e-210
Identity = 375/577 (64.99%), Postives = 455/577 (78.86%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG+ Y+  S A T LSF+GLQ+WTELS+D+LR++ +I +N     ++   ++LLL SY T
Sbjct: 1   MGIKYLPVSVASTALSFVGLQVWTELSLDRLRADGLIAKN-ISLGDSEHALELLLGSYFT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LL NF LNV++LL+L LKT+FF +LY  E++KLVERL NY+IYKGTFLPLVIPPT + 
Sbjct: 61  IALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANYIIYKGTFLPLVIPPTIFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW++WL VLC+LKMFQALARDRLERLNASPS+T WTYFRVYSVL LVL VD+  I+  
Sbjct: 121 GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTYFRVYSVLFLVLSVDMFWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LM Y T+G +++LLLLFEP SIAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF + 
Sbjct: 181 LMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFIDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHG+ F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGIAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           AI+KRI+ ++KL  ALGALHAALPDAT+EEL+AY+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 AILKRIKGYIKLRIALGALHAALPDATSEELRAYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR EN+VN R  E  SDEQLARQ    L RQN  +
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTENEVNPRTVEVSSDEQLARQ----LERQNNPV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
             L  G+FP +  + VE    R+  LDPSWL  W+SQG D AGPS  +R+VGLGRVQMMM
Sbjct: 421 HALATGLFPAEVPDSVENDTSRNLGLDPSWLQTWSSQGSDVAGPSTTSRTVGLGRVQMMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGSQVPRSVVGRHPGSMGGLNM-NVPRAS 540
           RHLASVGE+YAQT+++D+AW LWP+NPSQ +  S    +V   + G  GGL++  V   +
Sbjct: 481 RHLASVGESYAQTALDDAAWSLWPMNPSQASTSS---TTVPPGNGGRTGGLHLRTVSNTT 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSSCA 577
           N++L NILAMAETVREV+PHVPDE+IFQ     +S A
Sbjct: 541 NESLTNILAMAETVREVMPHVPDEIIFQDLQRTNSVA 569

BLAST of Tan0012034.3 vs. TAIR 10
Match: AT5G51450.1 (RPM1 interacting protein 3 )

HSP 1 Score: 716.1 bits (1847), Expect = 2.5e-206
Identity = 365/575 (63.48%), Postives = 450/575 (78.26%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG++Y+  S A T LSF+GLQ+WTELS+D+LR++ II +N     N+   ++LLLSS+ T
Sbjct: 1   MGITYLHISVATTALSFVGLQVWTELSLDRLRADGIITKN-ISLGNSENTLELLLSSHTT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LLA+F LN+++LL+L LKT+FF +LY+ E+RKLVERL NY+IYKGTFLP V+P T + 
Sbjct: 61  IALLASFVLNIYILLVLSLKTLFFGDLYAIETRKLVERLANYIIYKGTFLPFVVPRTVFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW+IWL VLC+LKMFQALARDRL+RLNASPS+T WTYFRVYS L +VL  DL  I+  
Sbjct: 121 GVLWTIWLTVLCTLKMFQALARDRLDRLNASPSSTPWTYFRVYSALFMVLSTDLCWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIY T+G S++LLLLFEP  IAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF++ 
Sbjct: 181 LMIYNTVGSSVYLLLLFEPCGIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFYDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHGM F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGMAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           +I+KRI+ ++KL  ALGALHAAL DAT+EEL+ Y+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 SILKRIKGYIKLRVALGALHAALLDATSEELRDYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR E++ N   GE  SDE LARQ      RQN ++
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTESEANPSRGEVSSDEHLARQ----FERQNNSV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPWRSTSLDPSWLHDWASQGVDGAGPSNAARSVGLGRVQMMM 480
             L  G+FP +T N  E  PWR++ +DPSWL  W+ QGVD  G S  +RSVGLG+VQ+MM
Sbjct: 421 HALTTGMFPTETPNFTESDPWRNSEVDPSWLQTWSDQGVDVVGSSAGSRSVGLGQVQLMM 480

Query: 481 RHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGS-QVPRSVVGRHPGSMGGLNMNVPRAS 540
           RHLASVGE  AQT+++D++WGLWP+NPSQ +  S  VP    GR     GGL++     +
Sbjct: 481 RHLASVGEGSAQTTLDDASWGLWPMNPSQASTSSTYVPPGAGGR----TGGLHLRTVSRA 540

Query: 541 NDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
            +N+A+ILAMAETVREVLPHVPDE+IFQ     +S
Sbjct: 541 ANNMASILAMAETVREVLPHVPDEIIFQDLQRTNS 566

BLAST of Tan0012034.3 vs. TAIR 10
Match: AT5G51450.2 (RPM1 interacting protein 3 )

HSP 1 Score: 704.9 bits (1818), Expect = 5.7e-203
Identity = 365/593 (61.55%), Postives = 450/593 (75.89%), Query Frame = 0

Query: 1   MGVSYIAFSAACTVLSFIGLQLWTELSIDKLRSEKIIGENPFQTENAGRVVDLLLSSYIT 60
           MG++Y+  S A T LSF+GLQ+WTELS+D+LR++ II +N     N+   ++LLLSS+ T
Sbjct: 1   MGITYLHISVATTALSFVGLQVWTELSLDRLRADGIITKN-ISLGNSENTLELLLSSHTT 60

Query: 61  VGLLANFALNVFVLLILFLKTIFFVELYSSESRKLVERLVNYVIYKGTFLPLVIPPTKYH 120
           + LLA+F LN+++LL+L LKT+FF +LY+ E+RKLVERL NY+IYKGTFLP V+P T + 
Sbjct: 61  IALLASFVLNIYILLVLSLKTLFFGDLYAIETRKLVERLANYIIYKGTFLPFVVPRTVFQ 120

Query: 121 AALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFRVYSVLLLVLFVDLSGIRFC 180
             LW+IWL VLC+LKMFQALARDRL+RLNASPS+T WTYFRVYS L +VL  DL  I+  
Sbjct: 121 GVLWTIWLTVLCTLKMFQALARDRLDRLNASPSSTPWTYFRVYSALFMVLSTDLCWIKLS 180

Query: 181 LMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQFLDIWLHHSAGNCSNCQISKFFNM 240
           LMIY T+G S++LLLLFEP  IAFETLQA+L+HGFQ LD+W++H A   S+CQ SKF++ 
Sbjct: 181 LMIYNTVGSSVYLLLLFEPCGIAFETLQALLIHGFQLLDMWINHLAVKNSDCQRSKFYDS 240

Query: 241 TIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIWRLHGMTFNLVDAVLFLNIRALLS 300
             AGSL E KG L RNLGFFLDMATL+MALGHY+HIW LHGM F+LVDAVLFLNIRALLS
Sbjct: 241 MTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWLHGMAFHLVDAVLFLNIRALLS 300

Query: 301 AIIKRIRSFMKLMKALGALHAALPDATTEELQAYNDECAICREPMAKAKKLNCNHLFHLA 360
           +I+KRI+ ++KL  ALGALHAAL DAT+EEL+ Y+DECAICREPMAKAK+L+CNHLFHL 
Sbjct: 301 SILKRIKGYIKLRVALGALHAALLDATSEELRDYDDECAICREPMAKAKRLHCNHLFHLG 360

Query: 361 CLRSWLDQGLNEYYSCPTCRKPLFVGRQENDVNHRAGETFSDEQLARQISAGLNRQNATI 420
           CLRSWLDQGLNE YSCPTCRKPLFVGR E++ N   GE  SDE LARQ      RQN ++
Sbjct: 361 CLRSWLDQGLNEVYSCPTCRKPLFVGRTESEANPSRGEVSSDEHLARQ----FERQNNSV 420

Query: 421 PTLPAGIFPNQTQNPVEGSPW------------------RSTSLDPSWLHDWASQGVDGA 480
             L  G+FP +T N  E  PW                  R++ +DPSWL  W+ QGVD  
Sbjct: 421 HALTTGMFPTETPNFTESDPWRLAFLNGISSSHLFLMPYRNSEVDPSWLQTWSDQGVDVV 480

Query: 481 GPSNAARSVGLGRVQMMMRHLASVGETYAQTSIEDSAWGLWPLNPSQVAGGS-QVPRSVV 540
           G S  +RSVGLG+VQ+MMRHLASVGE  AQT+++D++WGLWP+NPSQ +  S  VP    
Sbjct: 481 GSSAGSRSVGLGQVQLMMRHLASVGEGSAQTTLDDASWGLWPMNPSQASTSSTYVPPGAG 540

Query: 541 GRHPGSMGGLNMNVPRASNDNLANILAMAETVREVLPHVPDELIFQSASGVSS 575
           GR     GGL++     + +N+A+ILAMAETVREVLPHVPDE+IFQ     +S
Sbjct: 541 GR----TGGLHLRTVSRAANNMASILAMAETVREVLPHVPDEIIFQDLQRTNS 584

BLAST of Tan0012034.3 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 2.5e-17
Identity = 98/345 (28.41%), Postives = 152/345 (44.06%), Query Frame = 0

Query: 54  LLSSYITVGLLANFALNVFVLLILFLKTIFFVELYSSES--------RKLVERLVNYVIY 113
           L +S I++ LL N  L + + L   +K +F   L  +E         R+L+E L    I+
Sbjct: 36  LSTSKISLVLLLNMCLVLMLSLWHLVKFVFLGSLREAEVERLNEQAWRELMEILFAITIF 95

Query: 114 K----GTFLPLVIPPTKYHAALWSIWLAVLCSLKMFQALARDRLERLNASPSATSWTYFR 173
           +      FLPLV+                L  +K    LA+ R+E +  +PS +  ++FR
Sbjct: 96  RQDFSSGFLPLVV---------------TLLLIKALHWLAQKRVEYIETTPSVSKLSHFR 155

Query: 174 VYSVLLLVLFVD----LSGIRFCLMIYRTLGLSLFLLLLFEPLSIAFETLQAILVHGFQF 233
           + S +  +L VD     S IR  L+  R   +SLF    FE + +A  T+   + + F  
Sbjct: 156 IVSFMGFLLLVDSLFMYSSIRH-LIQSRQASVSLF--FSFEYMILATTTVAIFVKYVFYV 215

Query: 234 LDIWLHHSAGNCSNCQISKFFNMTIAGSLWELKGSLIRNLGFFLDMATLLMALGHYVHIW 293
            D+                     +    WE K      L    D+  L M +  +  I+
Sbjct: 216 TDM---------------------LMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIF 275

Query: 294 RLHGMTFNLVDAV--LFLNIRALLSAIIKRIRSFMKLMKALGALHAALPDATTEELQAYN 353
             +G+  +L+  +   F N +        R+  +++  K    ++   PDAT EEL A +
Sbjct: 276 MNYGVPLHLLRELYETFRNFQI-------RVSDYLRYRKITSNMNDRFPDATPEELTASD 330

Query: 354 DECAICREPMAKAKKLNCNHLFHLACLRSWLDQGLNEYYSCPTCR 381
             C ICRE M  AKKL C HLFH+ CLRSWL++      +CPTCR
Sbjct: 336 ATCIICREEMTNAKKLICGHLFHVHCLRSWLER----QQTCPTCR 330