Tan0008149.1 (mRNA) Snake gourd v1

Overview


Name	Tan0008149.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	metalloendoproteinase 2-MMP-like
Location	LG11: 5075931 .. 5077570 (+)
Sequence length	942
RNA-Seq Expression	Tan0008149.1
Synteny	Tan0008149.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGCCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCAGATGTCATCGAGGGCAAGACTGGAATGAATTCGGGCAAGAAAATGGTGAATCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGGTTCCAAATTCCACCTCACTTATGGTAAATTTAATCTTTATTTATATTCTTTCATCTTTAAACTCATATGTAGTTATCAGTCTTATTCAATGAGGAAATGATATATATATTTTTTTAAGTAAAATTGGAGTTGGATAATTCAAACTACAAATTTCGTAGTCATTAATACATTTGATACGCCAATTGAGCTATGATCTCTTTGGCATAACGAGAAAATGATATCAAGTTTGAATATTACAAGATCTTCTTTTCTAATACTATAATTCAAAAGTTTATACCAATAAGTTACAACAAAATTTATAGTGTGTTTGGAATAACTTGTTAAAAAAAACTTTTGGACAAAATTAATTTTTCTGTAAACACTTCTAAAAATAAAATTAATAATTTAAAGTGTGTTTAGTACAGTTTATTAAGTGATTTTAGATCATCAAAACTGATTTTAAAAAATAAATCTTAATAAATAAAAACTGCTTATTGGATTATTTTAAAAACTTTTTTGAAAGAAACCGGTGACAACTTTAGAGAGATTAATGAAACATTCTCCAAATATATAATAAAAAATAAAAAAAATTATAATTAATAGAATCATTGTGAAAACATTTATTATTTAAAATGTTTTTTAAAAAATATAAAATATAATAATATATTTTTTTTTTTAAAAAAAAATGTTTAAATTAGAAGGTATTCCAAAGAGACTAATATTCTATTTATATCCTTAACAGGTTTCCTTCCTGAAACCCCATCGGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACAGCTTGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTGGCTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCCATGCCTTCGATGGCGTTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACAGATGGGAGATTACATTTCGACGCCGTCGAGCCTTGGGCCGTCGGCGTCGTTCCTAACTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCATAGCTCCGTCGAAGGAGCTATCATGTGGCCAACTATCATGGACGGAGCTACCAAGGGTTTGCATGTCGACGATATTGCAGGCATTAATGCTTTATATAGTGCTACTTCTTGA

mRNA sequence

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGCCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCAGATGTCATCGAGGGCAAGACTGGAATGAATTCGGGCAAGAAAATGGTGAATCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGGTTCCAAATTCCACCTCACTTATGGTTTCCTTCCTGAAACCCCATCGGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACAGCTTGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTGGCTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCCATGCCTTCGATGGCGTTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACAGATGGGAGATTACATTTCGACGCCGTCGAGCCTTGGGCCGTCGGCGTCGTTCCTAACTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCATAGCTCCGTCGAAGGAGCTATCATGTGGCCAACTATCATGGACGGAGCTACCAAGGGTTTGCATGTCGACGATATTGCAGGCATTAATGCTTTATATAGTGCTACTTCTTGA

Coding sequence (CDS)

ATGGCATTTGAAGGATTATCATTCTTCTCTTTCTCTTTTTTCCTTCTAATTTCCATCTTCCCTTTAATATCATCTTCTCAGCCAAACCCTGCTCATAATTCCCATGGATTGGTGTTTCTAAACAATCTCAATGGATGCAAGAAGGGTGACAAAGTGGAAGGGATCCACCAAATGAAGAAATACCTCCAACATTTTGGCTATCTCAATCATAATCATTCTAGAGCTAATGATGATGAGTTCGATGAGCTCTTAGAGTCTGCCATTAAAACTTACCAAATCAACTACAATCTCAAGGCCACAGGTACTCTCGATGCCACGACGCTAGCTCTTATGTCGAAACCTCGATGCGGGGTTGCAGATGTCATCGAGGGCAAGACTGGAATGAATTCGGGCAAGAAAATGGTGAATCAACATAGGAAAATAAGTGGTCATTTCCACACAGTTTCTCACTTTTCTTTCTTCCAAGGAAACCCTAAATGGCCAGGTTCCAAATTCCACCTCACTTATGGTTTCCTTCCTGAAACCCCATCGGAGGCGATAGCTCCGGTGAGTCGAGCTTTTACAGCTTGGGCTGCGAACACTCACTTCACCTTCTCCCAAGCTTCTGGCTATCAAACAGCTGACATCAAAATAAGCTTTGAAAGTGGCGACCATGGAGATGGCCATGCCTTCGATGGCGTTGGAGGAGTCATAGCCCACGCCTTTTCTCCCACAGATGGGAGATTACATTTCGACGCCGTCGAGCCTTGGGCCGTCGGCGTCGTTCCTAACTCGTACGATGTCGAGACGGTGGCGTTGCATGAGATCGGACACCTTCTCGGCCTCCATCATAGCTCCGTCGAAGGAGCTATCATGTGGCCAACTATCATGGACGGAGCTACCAAGGGTTTGCATGTCGACGATATTGCAGGCATTAATGCTTTATATAGTGCTACTTCTTGA

Protein sequence

MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDDIAGINALYSATS

Homology

[Close]

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-61
Identity = 127/283 (44.88%), Postives = 166/283 (58.66%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRAN-DDEFDELLESAIKTYQINYNLKAT 101
           N  GC  G K +G++ +K+Y QHFGY+   +   N  D+FD++L++A++ YQ N+ L  T
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 102 GTLDATTLALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISG-HFHTVSHFSFFQGNPK 161
           G LD  TL  +  PRCG  DV+ G + M+SG+K         G  FH V H+SFF G P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 162 WPGSKFHLTYGFLPET--PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGD 221
           WP ++  LTY F P      E  +  SRAFT W   T  TF++   + T+DI I F SG+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 222 HGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG--------VVPNSYDVETVALHEI 281
           HGDG  FDG    +AHAFSP  G  H D  E W V          V  + D+E+VA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 282 GHLLGLHHSSVEGAIMWPTIMDGATK-GLHVDDIAGINALYSA 312
           GHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY A
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.1e-61
Identity = 135/322 (41.93%), Postives = 186/322 (57.76%), Query Frame = 0


Query: 8   FFSFSFFLLIS-----IFPLISSSQPNPAHNSHGLVF---LNNLNGCKKGDKVEGIHQMK 67
           F   S FL++S      FP  +S+   P+  +   VF    +N  GC  G  V+G++++K
Sbjct: 6   FGFLSLFLIVSPASAWFFP--NSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIK 65

Query: 68  KYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVA 127
           KY Q FGY+    S    D+FD++L++A++ YQ N+NL  TG LDA T+  +  PRCG  
Sbjct: 66  KYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 128 DVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETP--S 187
           DV+ G + M+ G++   +      H H V  ++ F G P+WP ++  LTY F P+ P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 188 EAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSP 247
           E  +  SRAF  W+  T   F+ +  + T+DI I F +GDHGDG  FDGV G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 248 TDGRLHFDAVEPWAVG-------VVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 307
             G+ H DA E W V         V  + D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

Query: 308 DGATK-GLHVDDIAGINALYSA 312
            G  K  L  DD+ GI  LY A
Sbjct: 306 TGKRKVDLTNDDVEGIQYLYGA 325

[Close]

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.8e-56
Identity = 126/281 (44.84%), Postives = 161/281 (57.30%), Query Frame = 0


Query: 41  NNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKAT 100
           + L GC  G+ + G+ ++K+Y + FGY+    +    D+FD++L+SAI TYQ N+NLK T
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT--DDFDDVLQSAINTYQKNFNLKVT 105

Query: 101 GTLDATTLALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKW 160
           G LD++TL  + KPRCG  D+I+G + MN GK +            T   +SFF G P+W
Sbjct: 106 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRW 165

Query: 161 PGSKFHLTYGFLPET--PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDH 220
           P  K  LTY F P+     E     SRAFT WA  T   F+++     ADI I F SG+H
Sbjct: 166 PKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEH 225

Query: 221 GDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG---------VVPNSYDVETVALHEI 280
           GDG  FDG  G +AHA SP  G LH D  E W +           V    D+E+VA+HEI
Sbjct: 226 GDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEI 285

Query: 281 GHLLGLHHSSVEGAIMWPTIMDGATK-GLHVDDIAGINALY 310
           GHLLGL HSSVE AIM+P I  G  K  L  DDI GI  LY
Sbjct: 286 GHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 117/271 (43.17%), Postives = 155/271 (57.20%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYL-NHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATT 108
           G   +G+  +K Y  H GY+ N  H    DD FD+ L SAIKTYQ NYNL  TG  D  T
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHF---DDNFDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 109 LALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHL 168
           L  +  PRCGV D+I            +N ++  S  F  +S ++FF+  P+W      L
Sbjct: 108 LKQIMTPRCGVPDII------------INTNKTTS--FGMISDYTFFKDMPRWQAGTTQL 167

Query: 169 TYGFLPETPSEAI--APVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFD 228
           TY F PE   +    + ++RAF+ W    +  F + + Y+TA+IKI F S +HGD + FD
Sbjct: 168 TYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFD 227

Query: 229 GVGGVIAHAFSPTDGRLHFDAVEPWAV------GVVPNSYDVETVALHEIGHLLGLHHSS 288
           G GG++ HAF+PTDGR HFDA E W          V +++D+E+VA+HEIGHLLGL HSS
Sbjct: 228 GPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSS 287

Query: 289 VEGAIMWPTIMDGATK-GLHVDDIAGINALY 310
              AIM+P+I     K  L  DDI GI  LY
Sbjct: 288 DLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.0e-51
Identity = 121/274 (44.16%), Postives = 158/274 (57.66%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTL 108
           G  V G+ ++K+YL  FGY+N + S    D FD  LESAI  YQ N  L  TG LD +T+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN-DGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTV 123

Query: 109 ALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHF-HTVSHFSFFQGNPKWPGSKFHL 168
            LMS PRCGV+D                 H  I+  F HT +H+++F G PKW  ++  L
Sbjct: 124 TLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NRDTL 183

Query: 169 TYGF-----LPETPSEAIAPV-SRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDG 228
           TY       L    SE +  V  RAF+ W++    +F +   + TAD+KI F +GDHGDG
Sbjct: 184 TYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDG 243

Query: 229 HAFDGVGGVIAHAFSPTDGRLHFDAVEPWAV-----GVVPNSYDVETVALHEIGHLLGLH 288
             FDGV G +AHAF+P +GRLH DA E W V     G    + D+E+VA HEIGHLLGL 
Sbjct: 244 LPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLG 303

Query: 289 HSSVEGAIMWPTIMDGATK-GLHVDDIAGINALY 310
           HSS E A+M+P++     K  L VDD+AG+  LY
Sbjct: 304 HSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Tan0008149.1 vs. NCBI nr
Match: XP_038889209.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 558.9 bits (1439), Expect = 2.7e-155
Identity = 273/316 (86.39%), Postives = 294/316 (93.04%), Query Frame = 0


Query: 1   MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKK 60
           MAFEGLSFF  SFFLLI IFPLISSS+PN AH SHGLVFLNNL+GCKKGDKVEGIHQMK 
Sbjct: 1   MAFEGLSFFITSFFLLIIIFPLISSSEPNNAH-SHGLVFLNNLHGCKKGDKVEGIHQMKA 60

Query: 61  YLQHFGYLNH--NHSRA-NDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCG 120
           YLQHFGYLNH  NHS++ +DDEFDE+LESAIKTYQ+NYNLKATGTLDATTLALMSKPRCG
Sbjct: 61  YLQHFGYLNHVQNHSKSFSDDEFDEVLESAIKTYQLNYNLKATGTLDATTLALMSKPRCG 120

Query: 121 VADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPS 180
           +ADV++GKT M SGKKMVNQHRKI+GHFH+VSH++FF+GNPKWP SK HLTYGFLP TPS
Sbjct: 121 IADVVDGKTMMKSGKKMVNQHRKINGHFHSVSHYAFFEGNPKWPASKSHLTYGFLPGTPS 180

Query: 181 EAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSP 240
           EA+APV RAFT WAANTHF+FSQAS Y+TADIKISFESGDHGDGHAFDGVGGVIAHAFSP
Sbjct: 181 EAVAPVGRAFTTWAANTHFSFSQASAYETADIKISFESGDHGDGHAFDGVGGVIAHAFSP 240

Query: 241 TDGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGL 300
           TDGRLHFDAVEPWA+G V +SYDVETVALHEIGHLLGLHHSSVEGAIMWPTIM GATKGL
Sbjct: 241 TDGRLHFDAVEPWAIGAVADSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMGGATKGL 300

Query: 301 HVDDIAGINALYSATS 314
           H DDIAGINALY+ TS
Sbjct: 301 HADDIAGINALYTTTS 315

[Close]

BLAST of Tan0008149.1 vs. NCBI nr
Match: XP_022993647.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 259/312 (83.01%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL 
Sbjct: 5   QALSFFASSLFLLITLFPLISSSPQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKRYLC 64

Query: 64  HFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADV 123
            FGYL+H  NHS+  DDEFDELLESAI+TYQ+NYNL+ATGTLDA+TLALMSKPRCGVADV
Sbjct: 65  RFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 IEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIA 183
             GKTGMNSGKKMVN+HRKIS HFH VSHF+FF GNPKWP SK HL YGFLP TP EAIA
Sbjct: 125 FHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIEAIA 184

Query: 184 PVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243
           PV RAFT WAANTHFTFSQAS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDD 303
           LHFDA+E WA+G VPNS+D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM+GATKGLH DD
Sbjct: 245 LHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYSATS 314
           IAGINALY+ATS
Sbjct: 305 IAGINALYTATS 315

[Close]

BLAST of Tan0008149.1 vs. NCBI nr
Match: XP_023551500.1 (metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 260/315 (82.54%), Postives = 281/315 (89.21%), Query Frame = 0


Query: 1   MAFEGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKK 60
           MA + LSFF  S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+
Sbjct: 1   MAVQSLSFFPSSLFLLITLFPLISSSHQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKR 60

Query: 61  YLQHFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGV 120
           YL  FGYLNH  NHS+  DDEFDELLESAI+TYQ+NYNL+ATGTLDA+TLALMSKPRCGV
Sbjct: 61  YLHRFGYLNHVQNHSKTYDDEFDELLESAIQTYQLNYNLEATGTLDASTLALMSKPRCGV 120

Query: 121 ADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSE 180
           ADV  GKT MNSGKKMVN+HRKI G FH VSHF+FF GNPKWP SK HLTYGFLP TP+E
Sbjct: 121 ADVFHGKTRMNSGKKMVNRHRKIGGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTE 180

Query: 181 AIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPT 240
           AIAPV RAFT WAANTHFTFSQAS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPT
Sbjct: 181 AIAPVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPT 240

Query: 241 DGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLH 300
           DGRLHFDAVEPWA+G VP+S+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH
Sbjct: 241 DGRLHFDAVEPWAIGAVPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLH 300

Query: 301 VDDIAGINALYSATS 314
            DDIAGINALY+ TS
Sbjct: 301 ADDIAGINALYTTTS 314

[Close]

BLAST of Tan0008149.1 vs. NCBI nr
Match: KAG6578618.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 255/312 (81.73%), Postives = 279/312 (89.42%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFFS S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFFSSSLFLLIALFPLISSSHQYPAH-SHGLLFLNNLNGCKKGDMVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ATGTLDA+TLALM KPRCGVADV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMLKPRCGVADV 124

Query: 124 IEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIA 183
             GKT MNSGKKMVN+HRKISG FH VSHF+FF GNPKWP SK HLTYGFLP TP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243
           PV RAFT WAANTHFTFS AS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDD 303
           LHFDAVEPWA+G +P+S+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH DD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYSATS 314
           IAGINALY+ TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0008149.1 vs. NCBI nr
Match: KAG7016165.1 (Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 523.9 bits (1348), Expect = 9.8e-145
Identity = 254/312 (81.41%), Postives = 279/312 (89.42%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PA  SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFFASSLFLLIALFPLISSSHQYPA-QSHGLLFLNNLNGCKKGDTVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ATGTLDA+TLALMSKPRCGVADV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 IEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIA 183
             GKT MNSGKKMVN+HRKISG FH VSHF+FF GNPKWP SK HLTYGFLP TP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243
           PV RAFT WAANTHFTFS AS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDD 303
           LHFDAVEPWA+G +P+S+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH DD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYSATS 314
           IAGINALY+ TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL
Match: A0A6J1JTF5 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.0e-147
Identity = 259/312 (83.01%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSFF+ S FLLI++FPLISSS   PAH SHGL+FLNNLNGCKKGD VEG+HQMK+YL 
Sbjct: 5   QALSFFASSLFLLITLFPLISSSPQYPAH-SHGLLFLNNLNGCKKGDTVEGLHQMKRYLC 64

Query: 64  HFGYLNH--NHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADV 123
            FGYL+H  NHS+  DDEFDELLESAI+TYQ+NYNL+ATGTLDA+TLALMSKPRCGVADV
Sbjct: 65  RFGYLSHVQNHSKTYDDEFDELLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 IEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIA 183
             GKTGMNSGKKMVN+HRKIS HFH VSHF+FF GNPKWP SK HL YGFLP TP EAIA
Sbjct: 125 FHGKTGMNSGKKMVNRHRKISRHFHGVSHFAFFDGNPKWPASKSHLAYGFLPGTPIEAIA 184

Query: 184 PVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243
           PV RAFT WAANTHFTFSQAS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSQASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDD 303
           LHFDA+E WA+G VPNS+D+ETVALHEIGHLLGLHHSSVEGAIMWPTIM+GATKGLH DD
Sbjct: 245 LHFDAIETWAIGAVPNSFDMETVALHEIGHLLGLHHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYSATS 314
           IAGINALY+ATS
Sbjct: 305 IAGINALYTATS 315

[Close]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL
Match: A0A6J1FDT9 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.4e-144
Identity = 253/312 (81.09%), Postives = 278/312 (89.10%), Query Frame = 0


Query: 4   EGLSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMKKYLQ 63
           + LSF + S FLLI++FPLISSS   PA  SHGL+FLNNLNGCKKGD VEG+HQMK+YL+
Sbjct: 5   QALSFLASSLFLLIALFPLISSSHQYPA-QSHGLLFLNNLNGCKKGDTVEGLHQMKRYLR 64

Query: 64  HFGYLN--HNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVADV 123
            FGYLN   NHS+  DDEFDE+LESAI+TYQ+NYNL+ATGTLDA+TLALMSKPRCGVADV
Sbjct: 65  RFGYLNRVQNHSKTYDDEFDEVLESAIRTYQLNYNLEATGTLDASTLALMSKPRCGVADV 124

Query: 124 IEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETPSEAIA 183
             GKT MNSGKKMVN+HRKISG FH VSHF+FF GNPKWP SK HLTYGFLP TP+EAIA
Sbjct: 125 FHGKTRMNSGKKMVNRHRKISGRFHGVSHFAFFDGNPKWPASKSHLTYGFLPGTPTEAIA 184

Query: 184 PVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSPTDGR 243
           PV RAFT WAANTHFTFS AS Y+TADIKISFESGDHGDG AFDGVGGVIAHAFSPTDGR
Sbjct: 185 PVGRAFTTWAANTHFTFSHASSYETADIKISFESGDHGDGEAFDGVGGVIAHAFSPTDGR 244

Query: 244 LHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKGLHVDD 303
           LHFDAVEPWA+G +P+S+D+ETVALHEIGHLLGL HSSVEGAIMWPTIM+GATKGLH DD
Sbjct: 245 LHFDAVEPWAIGAIPDSFDMETVALHEIGHLLGLQHSSVEGAIMWPTIMEGATKGLHADD 304

Query: 304 IAGINALYSATS 314
           IAGINALY+ TS
Sbjct: 305 IAGINALYTTTS 315

[Close]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL
Match: A0A1S3C9I6 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.1e-136
Identity = 243/316 (76.90%), Postives = 277/316 (87.66%), Query Frame = 0


Query: 1   MAFEG-LSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M FE  LSF + SFFL+I +FPLISSS PN  H+S+GL FL NLNGC+KGDKV GIHQ+K
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPN-IHHSNGLAFLKNLNGCRKGDKVAGIHQIK 60

Query: 61  KYLQHFGYLNH---NHSRA-NDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPR 120
           +YLQHFGYLN+   N+S++ +DDEFD +LESAIKTYQ+NYNLKATGTLDA TL LMSKPR
Sbjct: 61  EYLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPR 120

Query: 121 CGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPET 180
           CGVAD+I+GKT M SGKKMVNQHRKI+GHFHTVSH++FF GNPKWP SKFHLTYGFLP T
Sbjct: 121 CGVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGT 180

Query: 181 PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAF 240
           PS+A+APV RAF  W A+THF FS+AS Y+ ADIKISF SGDHGDGH+FDGVGGVIAH F
Sbjct: 181 PSKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGF 240

Query: 241 SPTDGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATK 300
           +PTDGRLHFDA+E WAVG V +S+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM+G TK
Sbjct: 241 APTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTK 300

Query: 301 GLHVDDIAGINALYSA 312
           GL  DDIAGINALY+A
Sbjct: 301 GLDADDIAGINALYNA 315

[Close]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL
Match: A0A0A0KS47 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.9e-136
Identity = 242/316 (76.58%), Postives = 267/316 (84.49%), Query Frame = 0


Query: 1   MAFEGLS-FFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M F+GLS FF+F+ FL+IS+FPLISSSQP P     GLVFL NL GCKKGDKVEGIHQMK
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKPV---DGLVFLKNLKGCKKGDKVEGIHQMK 60

Query: 61  KYLQHFGYLN---HNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRC 120
           KYLQHFGYLN   H  S ++DDEFDE+LE A+KTYQINYNLK TGTLD +TL  MSKPRC
Sbjct: 61  KYLQHFGYLNNDVHIRSESSDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETP 180
           GVAD+I GKT M SGK+ VNQHRKISGHFH VSHF+FF+GN +WP SK HLTYGFLP TP
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180

Query: 181 SEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFS 240
           SEAI+PV+RAFT WAANTHF+FSQ S Y+ ADIKISFE GDHGDG  FD VGGV+AHAF+
Sbjct: 181 SEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240

Query: 241 PTDGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATKG 300
           PTDGRLHFDAVE WA G VP SYD+ETVALHEIGHLLGLHHSSVEGAIMWP+IM GATKG
Sbjct: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300

Query: 301 LHVDDIAGINALYSAT 313
           LH DDI GI  LY+ +
Sbjct: 301 LHADDIEGIKVLYTTS 313

[Close]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL
Match: A0A5D3BMC5 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00180 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.0e-136
Identity = 242/316 (76.58%), Postives = 276/316 (87.34%), Query Frame = 0


Query: 1   MAFEG-LSFFSFSFFLLISIFPLISSSQPNPAHNSHGLVFLNNLNGCKKGDKVEGIHQMK 60
           M FE  LSF + SFFL+I +FPLISSS PN  H+S+GL FL NLNGC+KGDKV GIHQ+K
Sbjct: 1   MTFEKLLSFLTTSFFLVIVLFPLISSSHPN-IHHSNGLAFLKNLNGCRKGDKVAGIHQIK 60

Query: 61  KYLQHFGYLNH---NHSRA-NDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPR 120
           +YLQHFGYLN+   N+S++ +DDEFD +LESAIKTYQ+NYNLKATGTLDA TL LMSKPR
Sbjct: 61  EYLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPR 120

Query: 121 CGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPET 180
           CGVAD+I+GKT M SGKKMVNQHRKI+GHFHTVSH++FF GNPKWP SK HLTYGFLP T
Sbjct: 121 CGVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKSHLTYGFLPGT 180

Query: 181 PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAF 240
           PS+A+APV RAF  W A+THF FS+AS Y+ ADIKISF SGDHGDGH+FDGVGGVIAH F
Sbjct: 181 PSKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGF 240

Query: 241 SPTDGRLHFDAVEPWAVGVVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIMDGATK 300
           +PTDGRLHFDA+E WAVG V +S+D+ETVALHEIGHLLGLHHSSVEGAIMWP+IM+G TK
Sbjct: 241 APTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTK 300

Query: 301 GLHVDDIAGINALYSA 312
           GL  DDIAGINALY+A
Sbjct: 301 GLDADDIAGINALYNA 315

[Close]

BLAST of Tan0008149.1 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-62
Identity = 127/283 (44.88%), Postives = 166/283 (58.66%), Query Frame = 0


Query: 42  NLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRAN-DDEFDELLESAIKTYQINYNLKAT 101
           N  GC  G K +G++ +K+Y QHFGY+   +   N  D+FD++L++A++ YQ N+ L  T
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 102 GTLDATTLALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISG-HFHTVSHFSFFQGNPK 161
           G LD  TL  +  PRCG  DV+ G + M+SG+K         G  FH V H+SFF G P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 162 WPGSKFHLTYGFLPET--PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGD 221
           WP ++  LTY F P      E  +  SRAFT W   T  TF++   + T+DI I F SG+
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGE 227

Query: 222 HGDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG--------VVPNSYDVETVALHEI 281
           HGDG  FDG    +AHAFSP  G  H D  E W V          V  + D+E+VA+HEI
Sbjct: 228 HGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEI 287

Query: 282 GHLLGLHHSSVEGAIMWPTIMDGATK-GLHVDDIAGINALYSA 312
           GHLLGL HSSVEG+IM+PTI  G  K  L  DD+ G+  LY A
Sbjct: 288 GHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGA 330

[Close]

BLAST of Tan0008149.1 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 236.9 bits (603), Expect = 2.2e-62
Identity = 135/322 (41.93%), Postives = 186/322 (57.76%), Query Frame = 0


Query: 8   FFSFSFFLLIS-----IFPLISSSQPNPAHNSHGLVF---LNNLNGCKKGDKVEGIHQMK 67
           F   S FL++S      FP  +S+   P+  +   VF    +N  GC  G  V+G++++K
Sbjct: 6   FGFLSLFLIVSPASAWFFP--NSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIK 65

Query: 68  KYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKPRCGVA 127
           KY Q FGY+    S    D+FD++L++A++ YQ N+NL  TG LDA T+  +  PRCG  
Sbjct: 66  KYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNP 125

Query: 128 DVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGSKFHLTYGFLPETP--S 187
           DV+ G + M+ G++   +      H H V  ++ F G P+WP ++  LTY F P+ P   
Sbjct: 126 DVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTE 185

Query: 188 EAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGVGGVIAHAFSP 247
           E  +  SRAF  W+  T   F+ +  + T+DI I F +GDHGDG  FDGV G +AHAFSP
Sbjct: 186 EVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSP 245

Query: 248 TDGRLHFDAVEPWAVG-------VVPNSYDVETVALHEIGHLLGLHHSSVEGAIMWPTIM 307
             G+ H DA E W V         V  + D+E+VA+HEIGHLLGL HSSVE +IM+PTI 
Sbjct: 246 PSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTIT 305

Query: 308 DGATK-GLHVDDIAGINALYSA 312
            G  K  L  DD+ GI  LY A
Sbjct: 306 TGKRKVDLTNDDVEGIQYLYGA 325

[Close]

BLAST of Tan0008149.1 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-57
Identity = 126/281 (44.84%), Postives = 161/281 (57.30%), Query Frame = 0


Query: 41  NNLNGCKKGDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKAT 100
           + L GC  G+ + G+ ++K+Y + FGY+    +    D+FD++L+SAI TYQ N+NLK T
Sbjct: 46  SKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT--DDFDDVLQSAINTYQKNFNLKVT 105

Query: 101 GTLDATTLALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKW 160
           G LD++TL  + KPRCG  D+I+G + MN GK +            T   +SFF G P+W
Sbjct: 106 GKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRW 165

Query: 161 PGSKFHLTYGFLPET--PSEAIAPVSRAFTAWAANTHFTFSQASGYQTADIKISFESGDH 220
           P  K  LTY F P+     E     SRAFT WA  T   F+++     ADI I F SG+H
Sbjct: 166 PKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEH 225

Query: 221 GDGHAFDGVGGVIAHAFSPTDGRLHFDAVEPWAVG---------VVPNSYDVETVALHEI 280
           GDG  FDG  G +AHA SP  G LH D  E W +           V    D+E+VA+HEI
Sbjct: 226 GDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEI 285

Query: 281 GHLLGLHHSSVEGAIMWPTIMDGATK-GLHVDDIAGINALY 310
           GHLLGL HSSVE AIM+P I  G  K  L  DDI GI  LY
Sbjct: 286 GHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]

BLAST of Tan0008149.1 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.6e-52
Identity = 121/274 (44.16%), Postives = 158/274 (57.66%), Query Frame = 0


Query: 49  GDKVEGIHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTL 108
           G  V G+ ++K+YL  FGY+N + S    D FD  LESAI  YQ N  L  TG LD +T+
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN-DGSEIFSDVFDGPLESAISLYQENLGLPITGRLDTSTV 123

Query: 109 ALMSKPRCGVADVIEGKTGMNSGKKMVNQHRKISGHF-HTVSHFSFFQGNPKWPGSKFHL 168
            LMS PRCGV+D                 H  I+  F HT +H+++F G PKW  ++  L
Sbjct: 124 TLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--NRDTL 183

Query: 169 TYGF-----LPETPSEAIAPV-SRAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDG 228
           TY       L    SE +  V  RAF+ W++    +F +   + TAD+KI F +GDHGDG
Sbjct: 184 TYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDG 243

Query: 229 HAFDGVGGVIAHAFSPTDGRLHFDAVEPWAV-----GVVPNSYDVETVALHEIGHLLGLH 288
             FDGV G +AHAF+P +GRLH DA E W V     G    + D+E+VA HEIGHLLGL 
Sbjct: 244 LPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLG 303

Query: 289 HSSVEGAIMWPTIMDGATK-GLHVDDIAGINALY 310
           HSS E A+M+P++     K  L VDD+AG+  LY
Sbjct: 304 HSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]

BLAST of Tan0008149.1 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 179.1 bits (453), Expect = 5.5e-45
Identity = 108/267 (40.45%), Postives = 144/267 (53.93%), Query Frame = 0


Query: 55  IHQMKKYLQHFGYLNHNHSRANDDEFDELLESAIKTYQINYNLKATGTLDATTLALMSKP 114
           I ++K++LQ +GYL       N +  D   E A+  YQ N  L  TG  D+ TL+ +  P
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILLP 109

Query: 115 RCGVADVIEGKTGMNSGKKMVNQHRKISGHFHTVSHFSFFQGNPKWPGS-KFHLTYGFLP 174
           RCG  D +E KT                  FHT   + +F G P+W       LTY F  
Sbjct: 110 RCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQ 169

Query: 175 ETPSEAIAPVS------RAFTAWAANTHFTFSQASGYQTADIKISFESGDHGDGHAFDGV 234
           E  +  +AP        RAF  WA+    +F +   Y  ADIKI F +GDHGDG  FDGV
Sbjct: 170 ENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGV 229

Query: 235 GGVIAHAFSPTDGRLHFDAVEPWAVGVVPN----SYDVETVALHEIGHLLGLHHSSVEGA 294
            GV+AH FSP +GRLH D  E WAV         + D+E+VA+HEIGH+LGL HSSV+ A
Sbjct: 230 LGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDA 289

Query: 295 IMWPTIMDGATK-GLHVDDIAGINALY 310
            M+PT+   + K  L++DD+ G+ +LY
Sbjct: 290 AMYPTLKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:

BLAST of Tan0008149.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	1.8e-61	44.88	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	3.1e-61	41.93	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	1.8e-56	44.84	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
P29136	1.7e-51	43.17	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	5.0e-51	44.16	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Tan0008149.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889209.1	2.7e-155	86.39	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]
XP_022993647.1	2.1e-147	83.01	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]
XP_023551500.1	2.1e-147	82.54	metalloendoproteinase 2-MMP-like [Cucurbita pepo subsp. pepo]	[more]
KAG6578618.1	2.6e-145	81.73	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7016165.1	9.8e-145	81.41	Metalloendoproteinase 2-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of Tan0008149.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTF5	1.0e-147	83.01	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489576 PE=...	[more]
A0A6J1FDT9	2.4e-144	81.09	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111444634 P...	[more]
A0A1S3C9I6	1.1e-136	76.90	metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498171 PE=3 SV...	[more]
A0A0A0KS47	6.9e-136	76.58	ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G633160 PE=3 S...	[more]
A0A5D3BMC5	9.0e-136	76.58	Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Tan0008149.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	1.3e-62	44.88	Matrixin family protein	[more]
AT1G70170.1	2.2e-62	41.93	matrix metalloproteinase	[more]
AT1G59970.1	1.3e-57	44.84	Matrixin family protein	[more]
AT4G16640.1	3.6e-52	44.16	Matrixin family protein	[more]
AT2G45040.1	5.5e-45	40.45	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 111..124 score: 55.84 coord: 207..235 score: 58.66 coord: 183..198 score: 35.21 coord: 264..289 score: 57.69 coord: 297..310 score: 52.79
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 23..313 e-value: 5.3E-62 score: 207.9
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 156..311 e-value: 1.6E-31 score: 120.7
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 53..111 e-value: 1.6E-12 score: 47.4
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 160..309 e-value: 3.4E-43 score: 147.2
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 46..313 e-value: 2.9E-78 score: 264.7
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 13..310
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 13..310
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 153..310
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 159..309 e-value: 2.9452E-58 score: 182.789
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 57..121

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0008149	Tan0008149	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0008149.1-exon	Tan0008149.1-exon-LG11:5075931..5076438	exon
Tan0008149.1-exon	Tan0008149.1-exon-LG11:5077137..5077570	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0008149.1-cds	Tan0008149.1-cds-LG11:5075931..5076438	CDS
Tan0008149.1-cds	Tan0008149.1-cds-LG11:5077137..5077570	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0008149.1	Tan0008149.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
cellular_component	GO:0031012	extracellular matrix
cellular_component	GO:0031225	anchored component of membrane
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0008270	zinc ion binding