Tan0006953.1 (mRNA) Snake gourd v1

Overview


Name	Tan0006953.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calmodulin
Location	LG01: 114772368 .. 114773149 (-)
Sequence length	782
RNA-Seq Expression	Tan0006953.1
Synteny	Tan0006953.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAAAAAATCAATGGCCATTTCCACTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTCAATTTCCCTCACCTTCACTCCAAACACAAACCCACGTCGTCTAAACCTCCTCCTCCACCGCCCCTCACGGTGGCGCCACCGCTCGTCGAAGCTGCCTTTCGCCACCTTGATGCCGATGGAGATGGCAAAATCTCTAGCGACGAGCTCCGATCCTACTTTGCATCGATTGGTGAGTACATGACACGCGACAATGCTCGAAGTGTGATCGGAGACTTCGATGGCGACGGCGACGAGCTACTAGAGCTTCAAGATTTCGAACGACTGATAAAGTGCGAGGAAGAAGGAGAGGAGAAAGATGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAAGGGTGTGGATTCATTGAACCAGCAGGATTGCAAAAGATGTTTGAGAGACTTGGCTACGTCAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTTTTTGATGTTGACGGCGACGGTGTAGTTGATTATCGTGAATTTCTCAAAATGATGAATGCTTAATTTAACGTGTGAATAAATAAGAGCAAAACTTACCGGTGCAATCTAGACTGCATTCATATTTGTATATCTCCATTCCAATCACACCATATAAAAAAAAAAAAAATCTAAAATACCCATAAAAAGAAAAATAATTGTCCTATAATAAATTATAAACTAACATTTGAGTGATTTACATAAAAACTTAATTTTTTTTTAATAAATAATATCTTCTCATTGTTGTCA

mRNA sequence

GAAAAAAAAAATCAATGGCCATTTCCACTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTCAATTTCCCTCACCTTCACTCCAAACACAAACCCACGTCGTCTAAACCTCCTCCTCCACCGCCCCTCACGGTGGCGCCACCGCTCGTCGAAGCTGCCTTTCGCCACCTTGATGCCGATGGAGATGGCAAAATCTCTAGCGACGAGCTCCGATCCTACTTTGCATCGATTGGTGAGTACATGACACGCGACAATGCTCGAAGTGTGATCGGAGACTTCGATGGCGACGGCGACGAGCTACTAGAGCTTCAAGATTTCGAACGACTGATAAAGTGCGAGGAAGAAGGAGAGGAGAAAGATGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAAGGGTGTGGATTCATTGAACCAGCAGGATTGCAAAAGATGTTTGAGAGACTTGGCTACGTCAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTTTTTGATGTTGACGGCGACGGTGTAGTTGATTATCGTGAATTTCTCAAAATGATGAATGCTTAATTTAACGTGTGAATAAATAAGAGCAAAACTTACCGGTGCAATCTAGACTGCATTCATATTTGTATATCTCCATTCCAATCACACCATATAAAAAAAAAAAAAATCTAAAATACCCATAAAAAGAAAAATAATTGTCCTATAATAAATTATAAACTAACATTTGAGTGATTTACATAAAAACTTAATTTTTTTTTAATAAATAATATCTTCTCATTGTTGTCA

Coding sequence (CDS)

ATGGCCATTTCCACTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTCAATTTCCCTCACCTTCACTCCAAACACAAACCCACGTCGTCTAAACCTCCTCCTCCACCGCCCCTCACGGTGGCGCCACCGCTCGTCGAAGCTGCCTTTCGCCACCTTGATGCCGATGGAGATGGCAAAATCTCTAGCGACGAGCTCCGATCCTACTTTGCATCGATTGGTGAGTACATGACACGCGACAATGCTCGAAGTGTGATCGGAGACTTCGATGGCGACGGCGACGAGCTACTAGAGCTTCAAGATTTCGAACGACTGATAAAGTGCGAGGAAGAAGGAGAGGAGAAAGATGATTTGAAAATGGCTTTTGAGATGTTTGAAGTTGAGAAAGGGTGTGGATTCATTGAACCAGCAGGATTGCAAAAGATGTTTGAGAGACTTGGCTACGTCAAATCGAAAGAGGAGTGCATGGCTATGATCGGAGTTTTTGATGTTGACGGCGACGGTGTAGTTGATTATCGTGAATTTCTCAAAATGATGAATGCTTAA

Protein sequence

MAISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPPPLTVAPPLVEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMMNA

Homology

[Close]

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-28
Identity = 79/194 (40.72%), Postives = 106/194 (54.64%), Query Frame = 0


Query: 4   STPLKWFSKTNFKLNFPHLHSKHKPTS-SKPPPPPPLT------------VAPPLVEAAF 63
           S   KWFS    KLN    + +  P S S      P +             +   +   F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 64  RHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEE 123
            H D+DGDGKIS+ ELR YF S+GEY++ + A+  I + D D D  L  +DF  L+   +
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 124 ---EGEEKDD--LKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 180
              +GE   D  LK AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+D
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.1e-21
Identity = 50/134 (37.31%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 46  VEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERL 105
           +EA F ++DA+ DG+IS +EL+  F ++GE ++ + A + +   D DGD +L+ ++F +L
Sbjct: 44  LEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 103

Query: 106 IKCEEEGEEKDDLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 165
           IK ++E E+K +LK AF ++ + +G   I P  L+ M ++LG  ++ ++C  MI  FD++
Sbjct: 104 IKVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 163

Query: 166 GDGVVDYREFLKMM 180
            DGV+ + EF  MM
Sbjct: 164 ADGVLSFDEFALMM 176

[Close]

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.9e-19
Identity = 58/136 (42.65%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 50  FRHLDADGDGKISSDELRSYF-ASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKC 109
           F   D D DGKIS+ ELR    A++GE M+ + A +++   D D D LL+ ++F +L   
Sbjct: 12  FSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTKLAVQ 71

Query: 110 EEEGEEKD---DLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 169
            E G+E++    L  AF M+E+E G G I PA L++M  +LG  +  EEC  MI  FD+D
Sbjct: 72  LEMGDEEERCRGLMEAFRMYEME-GEGRITPASLKRMLSKLGSHQGIEECQTMICRFDLD 131

Query: 170 GDGVVDYREFLKMMNA 182
           GDGV+ + EF  MM+A
Sbjct: 132 GDGVISFEEFKIMMDA 146

[Close]

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-18
Identity = 58/139 (41.73%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 48  AAFRHLDADGDGKISSDELRSYF-ASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLI 107
           A F   D DGDG+IS+ ELR     ++GE ++ + A  ++   D DGD LL   +F RL+
Sbjct: 14  ALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLV 73

Query: 108 KCEEEGEEKD----DLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVF 167
           +  E  EE +     L+ AF M+E+E G G I P  L++M  RLG  +  ++C AMI  F
Sbjct: 74  QAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDCRAMICRF 133

Query: 168 DVDGDGVVDYREFLKMMNA 182
           D++GDGV+ + EF  MMNA
Sbjct: 134 DLNGDGVLSFDEFKIMMNA 151

[Close]

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.2e-18
Identity = 62/151 (41.06%), Postives = 87/151 (57.62%), Query Frame = 0


Query: 41  VAPPLVEAAFRHLDADGDGKISSDELR-SYFASIGEYMTRDNARSVIGDFDGDGDELLEL 100
           VA       F   D DGDGKIS+ ELR    AS+GE M  +  ++++   D DGD LL+ 
Sbjct: 2   VASDEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDE 61

Query: 101 QDFERLI-KCEEEGEEKDD--------LKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVK 160
           ++F RL+ + E +G+E++D        L+ AF M+E+E G G I P  L+ M  +LG   
Sbjct: 62  EEFVRLVTEMEADGDEEEDDDDETCRCLREAFAMYEME-GRGCITPLSLKLMLSKLGTHL 121

Query: 161 SKEECMAMIGVFDVDGDGVVDYREFLKMMNA 182
              EC AMI  FD++GDGV+ + EF  MM A
Sbjct: 122 DVAECQAMICRFDMNGDGVLTFDEFKTMMMA 151

[Close]

BLAST of Tan0006953.1 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 285.8 bits (730), Expect = 2.6e-73
Identity = 146/179 (81.56%), Postives = 155/179 (86.59%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP--PLTVAPPLVEAAFRHLDADGDGK 62
           ISTP KWFS TNFKLNFP L  KHKPT S PPPPP   LT APPL EAAFRHLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 63  ISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKM 122
           IS DEL+SYFAS+GEYMTRD+ARS+I +FDGDGD LLEL DFERL+K  EE EEK+DLKM
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEE-EEKEDLKM 139

Query: 123 AFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           AFEMFE EKGCGFI P GLQKMF RLGYVKS EECMAMIGVFDVDGDGV+DYREFL+MM
Sbjct: 140 AFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 197

[Close]

BLAST of Tan0006953.1 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-72
Identity = 145/179 (81.01%), Postives = 153/179 (85.47%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP--PLTVAPPLVEAAFRHLDADGDGK 62
           ISTP KWFS TN KLN P L  KHKPT S PPPPP   LT APPL EAAFRHLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 63  ISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKM 122
           IS DEL+SYFAS+GEYMTRDNARS+I +FDGDGD LLEL DFERL+K  EE EEK+DLK 
Sbjct: 66  ISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEE-EEKEDLKR 125

Query: 123 AFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           AFEMFE EKGCGFI PAGLQKMF RLGYVKS EECMAMIGVFDVDGDGV+DYREFL+MM
Sbjct: 126 AFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 183

[Close]

BLAST of Tan0006953.1 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 280.8 bits (717), Expect = 8.3e-72
Identity = 144/179 (80.45%), Postives = 153/179 (85.47%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP--PLTVAPPLVEAAFRHLDADGDGK 62
           ISTP KWFS TN KLN P L  KHKPT S PPPPP   LT APPL EAAFRHLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 63  ISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKM 122
           IS DEL+SYFAS+GEYMTRD+ARS+I +FDGDGD LLEL DFERL+K  EE EEK+DLK 
Sbjct: 66  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEE-EEKEDLKR 125

Query: 123 AFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           AFEMFE EKGCGFI PAGLQKMF RLGYVKS EECMAMIGVFDVDGDGV+DYREFL+MM
Sbjct: 126 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 183

[Close]

BLAST of Tan0006953.1 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.0 bits (715), Expect = 1.4e-71
Identity = 143/179 (79.89%), Postives = 153/179 (85.47%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP--PLTVAPPLVEAAFRHLDADGDGK 62
           +STP KWFS TNF LNFP L  KHKPT S PPPPP   LT APPL EAAFRHLDADGDGK
Sbjct: 43  VSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 102

Query: 63  ISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKM 122
           IS DEL+SYFAS+GEYMTRD+ARS+I +FD DGD LLEL DFERL+K  EE EEK+DLK 
Sbjct: 103 ISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKGGEE-EEKEDLKR 162

Query: 123 AFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           AFEMFE EKGCGFI PAGLQKMF RLGYVKS EECMAMIGVFDVDGDGV+DYREFL+MM
Sbjct: 163 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 220

[Close]

BLAST of Tan0006953.1 vs. NCBI nr
Match: XP_022936314.1 (probable calcium-binding protein CML41 [Cucurbita moschata])

HSP 1 Score: 278.1 bits (710), Expect = 5.4e-71
Identity = 142/178 (79.78%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP-PLTVAPPLVEAAFRHLDADGDGKI 62
           ISTP KWFS TN KLN P L  KHKPT S PPPPP  LT APPL EAAFRHLDADGDGKI
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDADGDGKI 65

Query: 63  SSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKMA 122
           S DEL+SYFAS+GEYMTRD+ARS+I +FDGDGD LLEL DFERL+K  E+ EEK+DLK A
Sbjct: 66  SGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQ-EEKEDLKRA 125

Query: 123 FEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           FEMFE EKGCGFI PAGLQKMF RLGYVKS EECMAMIGVFDVDG+GV+DYREFL+MM
Sbjct: 126 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFLRMM 182

[Close]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.3e-73
Identity = 146/179 (81.56%), Postives = 155/179 (86.59%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP--PLTVAPPLVEAAFRHLDADGDGK 62
           ISTP KWFS TNFKLNFP L  KHKPT S PPPPP   LT APPL EAAFRHLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 63  ISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKM 122
           IS DEL+SYFAS+GEYMTRD+ARS+I +FDGDGD LLEL DFERL+K  EE EEK+DLKM
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEE-EEKEDLKM 139

Query: 123 AFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           AFEMFE EKGCGFI P GLQKMF RLGYVKS EECMAMIGVFDVDGDGV+DYREFL+MM
Sbjct: 140 AFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 197

[Close]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.6e-71
Identity = 142/178 (79.78%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 3   ISTPLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPP-PLTVAPPLVEAAFRHLDADGDGKI 62
           ISTP KWFS TN KLN P L  KHKPT S PPPPP  LT APPL EAAFRHLDADGDGKI
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDADGDGKI 65

Query: 63  SSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDDLKMA 122
           S DEL+SYFAS+GEYMTRD+ARS+I +FDGDGD LLEL DFERL+K  E+ EEK+DLK A
Sbjct: 66  SGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQ-EEKEDLKRA 125

Query: 123 FEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLKMM 180
           FEMFE EKGCGFI PAGLQKMF RLGYVKS EECMAMIGVFDVDG+GV+DYREFL+MM
Sbjct: 126 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFLRMM 182

[Close]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 5.5e-69
Identity = 141/182 (77.47%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 1   MAIST---PLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPPPLTVAPPLVEAAFRHLDADG 60
           MAIST   P KWF+KTNFKLNFP LHSKHKP SSKPP P      PPL E AFRHLD DG
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP------PPLAEVAFRHLDIDG 60

Query: 61  DGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDD 120
           DGKIS DELRSYFASIGEYMT D A+SVIGDFD DGDELLEL DFERL+K EEE E+++D
Sbjct: 61  DGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEE-EQEED 120

Query: 121 LKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLK 180
           LK AFEMFE EKGCGFI   GLQKMF RLGYVKSKE+CM MI VFDVDGDGV+DY EFL+
Sbjct: 121 LKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLR 175

[Close]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 5.5e-69
Identity = 141/182 (77.47%), Postives = 150/182 (82.42%), Query Frame = 0


Query: 1   MAIST---PLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPPPLTVAPPLVEAAFRHLDADG 60
           MAIST   P KWF+KTNFKLNFP LHSKHKP SSKPP P      PPL E AFRHLD DG
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP------PPLAEVAFRHLDIDG 60

Query: 61  DGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKDD 120
           DGKIS DELRSYFASIGEYMT D A+SVIGDFD DGDELLEL DFERL+K EEE E+++D
Sbjct: 61  DGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEE-EQEED 120

Query: 121 LKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFLK 180
           LK AFEMFE EKGCGFI   GLQKMF RLGYVKSKE+CM MI VFDVDGDGV+DY EFL+
Sbjct: 121 LKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLR 175

[Close]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL
Match: A0A0A0LG17 (Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.1e-66
Identity = 139/183 (75.96%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   MAIST----PLKWFSKTNFKLNFPHLHSKHKPTSSKPPPPPPLTVAPPLVEAAFRHLDAD 60
           MAIS+    P KWF+KTNFKLNFP LHSKHKPTSSKP    P     PL E AFRHLD D
Sbjct: 1   MAISSIAVIPFKWFTKTNFKLNFPLLHSKHKPTSSKPSTSGP--GPRPLAEVAFRHLDVD 60

Query: 61  GDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEEEGEEKD 120
           GDGKIS +EL+SYFASIGEYMT ++ARSVIGDFD DGDELLEL DFER++K EE  EE++
Sbjct: 61  GDGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKGEE--EEEE 120

Query: 121 DLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVDGDGVVDYREFL 180
           DLK AFEMFE EKGCGFIE  GLQKMF RLGYVKSKEECMAMI VFDVDGDGV+DY EFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFL 179

[Close]

BLAST of Tan0006953.1 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 79/194 (40.72%), Postives = 106/194 (54.64%), Query Frame = 0


Query: 4   STPLKWFSKTNFKLNFPHLHSKHKPTS-SKPPPPPPLT------------VAPPLVEAAF 63
           S   KWFS    KLN    + +  P S S      P +             +   +   F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 64  RHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERLIKCEE 123
            H D+DGDGKIS+ ELR YF S+GEY++ + A+  I + D D D  L  +DF  L+   +
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 124 ---EGEEKDD--LKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 180
              +GE   D  LK AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+D
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of Tan0006953.1 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-22
Identity = 50/134 (37.31%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 46  VEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERL 105
           +EA F ++DA+ DG+IS +EL+  F ++GE ++ + A + +   D DGD +L+ ++F +L
Sbjct: 44  LEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 103

Query: 106 IKCEEEGEEKDDLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 165
           IK ++E E+K +LK AF ++ + +G   I P  L+ M ++LG  ++ ++C  MI  FD++
Sbjct: 104 IKVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 163

Query: 166 GDGVVDYREFLKMM 180
            DGV+ + EF  MM
Sbjct: 164 ADGVLSFDEFALMM 176

[Close]

BLAST of Tan0006953.1 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-22
Identity = 50/134 (37.31%), Postives = 88/134 (65.67%), Query Frame = 0


Query: 46  VEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERL 105
           +EA F ++DA+ DG+IS +EL+  F ++GE ++ + A + +   D DGD +L+ ++F +L
Sbjct: 44  LEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQL 103

Query: 106 IKCEEEGEEKDDLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 165
           IK ++E E+K +LK AF ++ + +G   I P  L+ M ++LG  ++ ++C  MI  FD++
Sbjct: 104 IKVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 163

Query: 166 GDGVVDYREFLKMM 180
            DGV+ + EF  MM
Sbjct: 164 ADGVLSFDEFALMM 176

[Close]

BLAST of Tan0006953.1 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 95.9 bits (237), Expect = 3.5e-20
Identity = 49/134 (36.57%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 46  VEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERL 105
           +EA F ++DA+ DG+IS +EL+  F ++GE +  D           DGD +L+ ++F +L
Sbjct: 44  LEAVFSYMDANRDGRISPEELQKSFMTLGEQLLSDT----------DGDGMLDFEEFSQL 103

Query: 106 IKCEEEGEEKDDLKMAFEMFEVEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFDVD 165
           IK ++E E+K +LK AF ++ + +G   I P  L+ M ++LG  ++ ++C  MI  FD++
Sbjct: 104 IKVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 163

Query: 166 GDGVVDYREFLKMM 180
            DGV+ + EF  MM
Sbjct: 164 ADGVLSFDEFALMM 166

[Close]

BLAST of Tan0006953.1 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-19
Identity = 48/136 (35.29%), Postives = 81/136 (59.56%), Query Frame = 0


Query: 46  VEAAFRHLDADGDGKISSDELRSYFASIGEYMTRDNARSVIGDFDGDGDELLELQDFERL 105
           +EA F ++DA+ DG+IS++EL+  F ++GE M+ + A + +   D DGD +L++ +F  L
Sbjct: 23  LEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALL 82

Query: 106 IKCEEEGEEKDDLKMAFEMFE--VEKGCGFIEPAGLQKMFERLGYVKSKEECMAMIGVFD 165
           IK  +E  E++  +   E F   +  G   I P  L+ M  +LG  ++ ++C  MI  FD
Sbjct: 83  IKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 142

Query: 166 VDGDGVVDYREFLKMM 180
           ++ DGV+ + EF  MM
Sbjct: 143 LNADGVLSFDEFALMM 158

The following BLAST results are available for this feature:

BLAST of Tan0006953.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	1.5e-28	40.72	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SRE6	3.1e-21	37.31	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYJ8	2.9e-19	42.65	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYK0	1.1e-18	41.73	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ9	3.2e-18	41.06	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Tan0006953.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022974919.1	2.6e-73	81.56	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
KAG7024217.1	1.7e-72	81.01	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]
KAG6591338.1	8.3e-72	80.45	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023535419.1	1.4e-71	79.89	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022936314.1	5.4e-71	79.78	probable calcium-binding protein CML41 [Cucurbita moschata]	[more]

BLAST of Tan0006953.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IIY2	1.3e-73	81.56	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	2.6e-71	79.78	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UHX6	5.5e-69	77.47	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	5.5e-69	77.47	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]
A0A0A0LG17	1.1e-66	75.96	Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1	[more]

BLAST of Tan0006953.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	1.1e-29	40.72	calmodulin-like 41	[more]
AT1G76650.1	2.2e-22	37.31	calmodulin-like 38	[more]
AT1G76650.3	2.2e-22	37.31	calmodulin-like 38	[more]
AT1G76650.2	3.5e-20	36.57	calmodulin-like 38	[more]
AT1G76640.1	2.3e-19	35.29	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 154..181 e-value: 0.67 score: 19.0 coord: 81..109 e-value: 27.0 score: 6.3 coord: 45..73 e-value: 3.9E-5 score: 33.1
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 154..180 e-value: 1.3E-6 score: 27.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 48..106 e-value: 5.0E-10 score: 39.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..181 score: 10.775421
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 41..76 score: 13.341799
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..179 e-value: 2.35282E-9 score: 49.4685
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 46..107 e-value: 1.6654E-10 score: 52.5501
None	No IPR available	GENE3D	1.10.238.10		coord: 109..181 e-value: 6.6E-16 score: 60.1
None	No IPR available	GENE3D	1.10.238.10		coord: 12..108 e-value: 1.8E-14 score: 56.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..41
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 17..180
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 17..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 54..66
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 47..179

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0006953	Tan0006953	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006953.1-three_prime_utr	Tan0006953.1-three_prime_utr-LG01:114772368..114772589	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006953.1-exon	Tan0006953.1-exon-LG01:114772368..114773149	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006953.1-cds	Tan0006953.1-cds-LG01:114772590..114773135	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006953.1-five_prime_utr	Tan0006953.1-five_prime_utr-LG01:114773136..114773149	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0006953.1	Tan0006953.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity