Tan0006536.1 (mRNA) Snake gourd v1

Overview


Name	Tan0006536.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pectinesterase
Location	LG04: 87782835 .. 87786319 (+)
Sequence length	2062
RNA-Seq Expression	Tan0006536.1
Synteny	Tan0006536.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCACGCATTTCCTTTTCAGTTTTTCAATTTCTAAACCCTAAATTCTCTCTCTGTTTCTCTGTCTCTTCACTTCATTCATCAAGTTGCAGAACCAAACTCCAACGCCATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGTCGGAAGACTCGACGTCGATTTATAATTCTCGTTGTCTCTGTTGTTCTTCTAATTGCTGTTGTCATTGGCGCCGTGGTCGGAATCGTGATTCATAAACGGAACTCTTCGTCTTCATCGGCGACGAACTCGGGTCCTCCGACGGAGCTCACTCCGGCCGCCTCACTCAAAACTCTCTGCAGTGTCACTCAGTATCCGAGTTCGTGCCAATCCTCTCTCGGAAACTCCAACACCACCGATCCGGTGTTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACCGATTCGCTCTCTAAACTCGCCGATTACTCCTCCAAGCTCAATTCCACTACTACTGATCCGAATCTCCGAGCCGCAATCGGAGTCTGCCTGAGTGTTTTCGAAGATGCTATAGAGACACTAAACGATACCGTTTCGTCGATGGAAGTGGATCGAAATGGTGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACCATCACCGACCAAGAAACCTGCCTCGACGCCGTTCGAGATCTGAACCAGACGGCGGTTCTCCAAGAATTACAAACGGCAATGGCGAACTCGACCGAGTTCACCAGCAATAGTTTAGCGATTGTAACGAAAATCCTGGGGCTGTTGTCGGATTTCAACATCCCAATCCACCGGAGGCTGATGGGGTTTCCGGAGTGGGTGAGCTCCGGAGACCGGAGGCTTCTGCAGGAGAAGAATGTGACGGCGCATGTGACGGTGTCGAAGGACGGGAATGGGGATTTCACGACGATCAAAGCCGCCGTAGCGGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATATATGTAAAAGAAGGAATATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGAAGAACCAGAACCATTATTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGGTAACCCATTTAATTATTTTCTTTTTCTTAATTGCTTCATTTAATTTGAGTTAAATTCTCCGTAATCCTCTTAAATTTTATGCATAGGTTTCAATTATGTCTAACTTTGAAAAGTTAACCCTTTGTTTTAAAAAAACCTTATCATTAGATTGATTTCAATTATATCCCCCACTTTAAAAAGTTTTATTTTTACTTTTGAATTTGGAAGTTTTTTTCTAAACCATCCTTAAATTTATGGATGGAAAATAATTTTTCTTGCATATGTTTTAATTTATTTTTCAATGTACATGTTTAAATATGATCACATAAGCAACAATAAAATTGCAAACATAATATTTTTCGTTCCTAAATTGAATAAAATAAATCTCTAAAAAATTGTTTTGAAACAAATTTCAAAATTCAAGAGTTAAAATGAAATTTTTTAAAGTTTGGGTATAATTGAAATCAACTTAAACTAAAGGATATTTTTTATGATTTCACATTTTTCCATTAAACAAAATTATTCTAAAAATTAGAATGCATATTGGTATTGACATCATTTTTAATGCCATTCATATAAGTCTTAAGTTTTACACCTCAACTACCACCCAAAAGTTCTTTTAAATAGGTTAGGCTATAAATTTAGTTTATAAACTTTAATATTTAGGTCTAATAGTTGTTAAAACTTTAAATAATATCTAATAAGTTTTTGAACTTTGAATTTTATATCTGGTAGATTTCTAAACTTTAAAAATGTCAAATAGGTTTTTAAACTTTTAATTTTGTGTCCAATAGATCTCAAATATTTCTAGACTTTTTATAATTAATAAATTCACTCAACATAAAATTGGTATAACTTATAAACTTATTAGAGACAAAATCAAAAGTGTAGGAAATATTTAATAACTATTTGGTCTTTGAAAGTAAAGCTTATAATTACTTTTTTTTTTTGTAATTTTTTGAAAAAAAAAACCAAGCCAAAGGAAAATTTTAAAAAGCTATTCAAATGTTTACTTAGAAGAGGAAAAATATTATAGAGAAATTATGAAAAAAAACAAATTTAATTTTAAAAAAATCAAAAATCAAAATTCAAATTGTTATTAAATAAAGCCATAGTAATTTATTAAACACTTCTATAAATTCTATTATAATACAAAATTAATTAAAAATTGGAAGACATGTTAAATATTTTTTAATTTAAGGAACTAATTTTAACTCTCTTTTTTTTTTTTTTTTTATAATATAAACTTTAATTTACTTTTGCAAAATGCATCCATGGCTGCCACGTTGTTATTCCACTTTTTGTTTGCCTTTTTGTTTAACCATCGGCCCCAAAAGGCCAATTCCCCTTATCGTCAGAAAGCCACATGGGCAGGGCAACGAGTCAGCCATGTGAAACCCAATAATGTTCTTCTCATCAATTTTGTTTGGTTTTTGCTTCCAGCTGTGGCTGGCAAAGGCTTCATTGGCAAGGACATGGGCTTCATCAACACCGCCGGTCCGGCCAAGCATCAGGCCGTCGCTTTCCGCTCCGGCTCCGATCTCTCTGTTATGTCCGGATGCTCATTCGATGCCTATCAAGACACTCTCTACGCTCATTCCAACCGTCAATTCTATCGAGACTGCGACATCACCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGTCGTCTTCCAGAGCTGCAACATCCGGCCGAGGCAGCCGCTTCCCAATCAGTTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCTGGCATTTCCATTCAGAAATGCTCTTTTTCGGCCTACAACGATAGTCTCTCCGCCCCGACTTACTTGGGCCGGCCATGGAAAGAATTCTCGACCACCGTGATTATGCAGTCGGAGATCGGCGATTTCCTGCAGCCGGTCGGGTGGAAGGAGTGGGTCAGCGGGCAGGACCCGCCAAGCACTATATTTTACGGCGAGTATCGGAATAGTGGGCCTGGATCCAACGTGGATAAAAGAGTTAAATGGGTTGGTTACAGGCCCAGTCTTACTGACGCCGATGCCGGAAAGTTCACCGTCGGAACCTTCCTCAACGGCCAGGATTGGTTGCCGGCCACCAGTGTAAAATTTGATTCGTCCTTATGATGAACCCATAGAAGAAAAATCACACGTTGAAAAGTTTAAATTAATATAATTTTTTTTTCTTGCAATTATCTTCTTTTGTTACATTACATCCAAGTGTGAAGTACGATTAGACTTGAAAGTTTTTTCTAGGACAACAATATAAATATAAATGTGGAGATTTGAATATTTACCTTTTGGTATTATGTTGTAACTAGTTATCCTATGTTTAAGTCGATAATTATGAC

mRNA sequence

GCCACGCATTTCCTTTTCAGTTTTTCAATTTCTAAACCCTAAATTCTCTCTCTGTTTCTCTGTCTCTTCACTTCATTCATCAAGTTGCAGAACCAAACTCCAACGCCATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGTCGGAAGACTCGACGTCGATTTATAATTCTCGTTGTCTCTGTTGTTCTTCTAATTGCTGTTGTCATTGGCGCCGTGGTCGGAATCGTGATTCATAAACGGAACTCTTCGTCTTCATCGGCGACGAACTCGGGTCCTCCGACGGAGCTCACTCCGGCCGCCTCACTCAAAACTCTCTGCAGTGTCACTCAGTATCCGAGTTCGTGCCAATCCTCTCTCGGAAACTCCAACACCACCGATCCGGTGTTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACCGATTCGCTCTCTAAACTCGCCGATTACTCCTCCAAGCTCAATTCCACTACTACTGATCCGAATCTCCGAGCCGCAATCGGAGTCTGCCTGAGTGTTTTCGAAGATGCTATAGAGACACTAAACGATACCGTTTCGTCGATGGAAGTGGATCGAAATGGTGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACCATCACCGACCAAGAAACCTGCCTCGACGCCGTTCGAGATCTGAACCAGACGGCGGTTCTCCAAGAATTACAAACGGCAATGGCGAACTCGACCGAGTTCACCAGCAATAGTTTAGCGATTGTAACGAAAATCCTGGGGCTGTTGTCGGATTTCAACATCCCAATCCACCGGAGGCTGATGGGGTTTCCGGAGTGGGTGAGCTCCGGAGACCGGAGGCTTCTGCAGGAGAAGAATGTGACGGCGCATGTGACGGTGTCGAAGGACGGGAATGGGGATTTCACGACGATCAAAGCCGCCGTAGCGGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATATATGTAAAAGAAGGAATATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGAAGAACCAGAACCATTATTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTGGCTGGCAAAGGCTTCATTGGCAAGGACATGGGCTTCATCAACACCGCCGGTCCGGCCAAGCATCAGGCCGTCGCTTTCCGCTCCGGCTCCGATCTCTCTGTTATGTCCGGATGCTCATTCGATGCCTATCAAGACACTCTCTACGCTCATTCCAACCGTCAATTCTATCGAGACTGCGACATCACCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGTCGTCTTCCAGAGCTGCAACATCCGGCCGAGGCAGCCGCTTCCCAATCAGTTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCTGGCATTTCCATTCAGAAATGCTCTTTTTCGGCCTACAACGATAGTCTCTCCGCCCCGACTTACTTGGGCCGGCCATGGAAAGAATTCTCGACCACCGTGATTATGCAGTCGGAGATCGGCGATTTCCTGCAGCCGGTCGGGTGGAAGGAGTGGGTCAGCGGGCAGGACCCGCCAAGCACTATATTTTACGGCGAGTATCGGAATAGTGGGCCTGGATCCAACGTGGATAAAAGAGTTAAATGGGTTGGTTACAGGCCCAGTCTTACTGACGCCGATGCCGGAAAGTTCACCGTCGGAACCTTCCTCAACGGCCAGGATTGGTTGCCGGCCACCAGTGTAAAATTTGATTCGTCCTTATGATGAACCCATAGAAGAAAAATCACACGTTGAAAAGTTTAAATTAATATAATTTTTTTTTCTTGCAATTATCTTCTTTTGTTACATTACATCCAAGTGTGAAGTACGATTAGACTTGAAAGTTTTTTCTAGGACAACAATATAAATATAAATGTGGAGATTTGAATATTTACCTTTTGGTATTATGTTGTAACTAGTTATCCTATGTTTAAGTCGATAATTATGAC

Coding sequence (CDS)

ATGGATTCCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGTCGGAAGACTCGACGTCGATTTATAATTCTCGTTGTCTCTGTTGTTCTTCTAATTGCTGTTGTCATTGGCGCCGTGGTCGGAATCGTGATTCATAAACGGAACTCTTCGTCTTCATCGGCGACGAACTCGGGTCCTCCGACGGAGCTCACTCCGGCCGCCTCACTCAAAACTCTCTGCAGTGTCACTCAGTATCCGAGTTCGTGCCAATCCTCTCTCGGAAACTCCAACACCACCGATCCGGTGTTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACCGATTCGCTCTCTAAACTCGCCGATTACTCCTCCAAGCTCAATTCCACTACTACTGATCCGAATCTCCGAGCCGCAATCGGAGTCTGCCTGAGTGTTTTCGAAGATGCTATAGAGACACTAAACGATACCGTTTCGTCGATGGAAGTGGATCGAAATGGTGAGAAGTTCTTGTCGCCGTCGAGGATTGAAGATCTGAAAACCTGGCTGAGCACCACCATCACCGACCAAGAAACCTGCCTCGACGCCGTTCGAGATCTGAACCAGACGGCGGTTCTCCAAGAATTACAAACGGCAATGGCGAACTCGACCGAGTTCACCAGCAATAGTTTAGCGATTGTAACGAAAATCCTGGGGCTGTTGTCGGATTTCAACATCCCAATCCACCGGAGGCTGATGGGGTTTCCGGAGTGGGTGAGCTCCGGAGACCGGAGGCTTCTGCAGGAGAAGAATGTGACGGCGCATGTGACGGTGTCGAAGGACGGGAATGGGGATTTCACGACGATCAAAGCCGCCGTAGCGGCGGTGCCAAAGAAGAGTAAAGAAAGATTCATCATATATGTAAAAGAAGGAATATACGAAGAGAATGTGATTCTGGATAAGAGCAAGTGGAATGTTATGATGTACGGAGATGGAAGAACCAGAACCATTATTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTGGCTGGCAAAGGCTTCATTGGCAAGGACATGGGCTTCATCAACACCGCCGGTCCGGCCAAGCATCAGGCCGTCGCTTTCCGCTCCGGCTCCGATCTCTCTGTTATGTCCGGATGCTCATTCGATGCCTATCAAGACACTCTCTACGCTCATTCCAACCGTCAATTCTATCGAGACTGCGACATCACCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGTCGTCTTCCAGAGCTGCAACATCCGGCCGAGGCAGCCGCTTCCCAATCAGTTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCTGGCATTTCCATTCAGAAATGCTCTTTTTCGGCCTACAACGATAGTCTCTCCGCCCCGACTTACTTGGGCCGGCCATGGAAAGAATTCTCGACCACCGTGATTATGCAGTCGGAGATCGGCGATTTCCTGCAGCCGGTCGGGTGGAAGGAGTGGGTCAGCGGGCAGGACCCGCCAAGCACTATATTTTACGGCGAGTATCGGAATAGTGGGCCTGGATCCAACGTGGATAAAAGAGTTAAATGGGTTGGTTACAGGCCCAGTCTTACTGACGCCGATGCCGGAAAGTTCACCGTCGGAACCTTCCTCAACGGCCAGGATTGGTTGCCGGCCACCAGTGTAAAATTTGATTCGTCCTTATGA

Protein sequence

MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSSSATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIPIHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL

Homology

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BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 6.3e-209
Identity = 371/586 (63.31%), Postives = 457/586 (77.99%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD++KSFKGYGKV+ELEQQA+ +KTR+R II+ VS ++LIAV+I AV G+VIH RNS SS
Sbjct: 1   MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGN---SNTTDPVFLFKLSLRVATDSL 120
            +++S P TEL+PAASLK +C  T+YPSSC SS+ +   SNTTDP  LFKLSLRVA D L
Sbjct: 61  PSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDEL 120

Query: 121 SKLADYSSKLNSTT-TDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRI 180
           S    + SKL +    D  L+ AI VC SVF DA++ LND++S++       +  S + +
Sbjct: 121 S---SFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAG--RIASSASV 180

Query: 181 EDLKTWLSTTITDQETCLDAVRDLNQTA---VLQELQTAMANSTEFTSNSLAIVTKILGL 240
            +++TWLS  +TDQ+TCLDAV +LN TA    LQE++TAM NSTEF SNSLAIVTKILGL
Sbjct: 181 SNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 240

Query: 241 LSDFNIPI-HRRLMGFPEWVSSGDRRLLQEKN--VTAHVTVSKDGNGDFTTIKAAVAAVP 300
           LS F  PI HRRL+GFPEW+ + +RRLL+EKN   T    V+KDG+G F TI  A+  V 
Sbjct: 241 LSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVK 300

Query: 301 KKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFA 360
           KKS+ERF +YVKEG Y EN+ LDK+ WNVM+YGDG+ +T + G  NF+DGTPTF TATFA
Sbjct: 301 KKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFA 360

Query: 361 VAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDC 420
           V GKGFI KD+GF+N AG +KHQAVA RSGSD SV   CSFD +QDTLYAHSNRQFYRDC
Sbjct: 361 VKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDC 420

Query: 421 DITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYN 480
           DITGTIDFIFGNAAVVFQSC I PRQPLPNQFNTITAQGKKD NQN+GI IQK + + + 
Sbjct: 421 DITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFG 480

Query: 481 DSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGS 540
           ++L+APTYLGRPWK+FSTTVIMQS+IG  L PVGW  WV   +PP+TIFY EY+NSGPG+
Sbjct: 481 NNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGA 540

Query: 541 NVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           +V +RVKW GY+P++TD +A +FTV +F+ G +WLP  +V+FDS+L
Sbjct: 541 DVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

[Close]

BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 1.3e-198
Identity = 357/590 (60.51%), Postives = 442/590 (74.92%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+R ++L +SVV+LIAV+I AVV  V+HK  + S+
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNEST 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSS---LGNSNTTDPVFLFKLSLRVATDSL 120
            +    PP ELTP+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D L
Sbjct: 61  PS----PPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIE 180
             ++D   KL+  T D  +++A+ VC  + EDA++ LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDAVRDLNQ-------TAVLQELQTAMANSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D++ +L Q       + + Q L++AM+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLSDFNIPIHRR----------LMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFT 300
           L  LSD  IPIHRR           + F +W     RRLLQ   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKAAVAAVPKKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDG 360
           T+  AVA VPKKS + F+IYVK G Y ENV++DKSKWNVM+YGDG+ +TIISG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYA 420
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFD +QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCSFSAYNDSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFY 540
           IQ+C+ SA N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP++I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYRNSGPGSNVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSV 571
           GEY+N+GPGS+V +RVKW GY+P ++DA+A KFTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

[Close]

BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.4e-160
Identity = 297/527 (56.36%), Postives = 372/527 (70.59%), Query Frame = 0


Query: 52  IHKRNSSSSSATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLG----NSNTTDPVFLFK 111
           IH+R+   S      P    TP++ L+T+C+VT YP+SC SS+     +  TTDP  LF+
Sbjct: 436 IHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFR 495

Query: 112 LSLRVATDSLSKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSM-EVDRN 171
           LSL+V  D L+ +     KL   T D  L++A+ VC  VF+ A++++NDT+SS+ EV   
Sbjct: 496 LSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISG 555

Query: 172 GEKFLSPSRIEDLKTWLSTTITDQETCLDAVRDLN-QTAVLQELQTAMANSTEFTSNSLA 231
           G+K L+ S I DL TWLS+ +TD  TC D + + N  + + Q+L++AM NSTEFTSNSLA
Sbjct: 556 GKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLA 615

Query: 232 IVTKILGLLSDFNIPIH-RRLM---GFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTT 291
           IV ++L   S   IP+  RRL+    FP WV  G RRLLQ KN+T HVTV+ DG+GD  T
Sbjct: 616 IVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVRT 675

Query: 292 IKAAVAAVPKKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGT 351
           +  AV  VPKK K  F+IYVK G Y ENV++ K KWNV +YGDGR +TIISG  N +DG 
Sbjct: 676 VNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGV 735

Query: 352 PTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAH 411
            TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFD YQDTLY H
Sbjct: 736 RTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTH 795

Query: 412 SNRQFYRDCDITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISI 471
           SNRQ+YR+CD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GISI
Sbjct: 796 SNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISI 855

Query: 472 QKCSFSAYNDSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQD-PPSTIFY 531
            +C+ S  N +++A TYLGRPWK FS TVIMQS IG F+ P GW  W S  D PP TIFY
Sbjct: 856 HQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFY 915

Query: 532 GEYRNSGPGSNVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQD-WLP 567
            EY+NSGPGS++ KRVKW GY+P  +D +A +FTV  FL G D W+P
Sbjct: 916 REYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

HSP 2 Score: 213.4 bits (542), Expect = 6.8e-54
Identity = 129/253 (50.99%), Postives = 174/253 (68.77%), Query Frame = 0


Query: 1   MDSVKSF-KGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSS 60
           MD+VKS  KGYGKVDE +  A +RKTR+R   + +SV +L+A++I + V I IH R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 61  SSATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGN---SNTTDPVFLFKLSLRVATDS 120
              T S  P ELTPAASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 121 LSKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRI 180
           L+ + +   KL   T D  L++A+ VC  + + AI+ +N+TVS+MEV  +G+K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 181 EDLKTWLSTTITDQETCLDAVRDLNQT--AVLQELQTAMANSTEFTSNSLAIVTKILGLL 240
           +DL TWLS  +T   TCLDA+ +++ T  A+  +L++ M NSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 241 SDFNIPIH-RRLM 247
           SDF IPIH RRL+
Sbjct: 241 SDFGIPIHGRRLL 251

[Close]

BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.4e-138
Identity = 278/579 (48.01%), Postives = 379/579 (65.46%), Query Frame = 0


Query: 10  YGKVDELEQQAFR--RKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSSSATNSGP 69
           YG++DE EQ      RKT++R  I+ +S ++L+ +V+GAVVG    + NS      N+G 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTA-RDNSKKPPTENNGE 63

Query: 70  PTELTPAASLKTLCSVTQYPSSCQSSLG---NSNTTDPVFLFKLSLRVATDSLSKLADYS 129
           P     + S+K LC VT +   C  +LG   N++ + P  LFK +++V    LSK+ D  
Sbjct: 64  PI----SVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 SKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLS 189
           S  N    D    AA+G C+ +   A++ LN+T++S           S    +DL+TWLS
Sbjct: 124 S--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIPIHRR 249
           +  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG ++D      RR
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFRRRR 243

Query: 250 LM--GFPEWVSSG-----DRRLLQEKNV--TAHVTVSKDGNGDFTTIKAAVAAVPKKSKE 309
           L+  G  + V +       RRLL+  ++   A + V+KDG+G + TI  A+A V +K+++
Sbjct: 244 LLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEK 303

Query: 310 RFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKG 369
             IIYVK+G+Y ENV ++K+KWNV+M GDG+++TI+S  LNFIDGTPTF TATFAV GKG
Sbjct: 304 PTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKG 363

Query: 370 FIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGT 429
           F+ +DMGFINTAGPAKHQAVA    +DLSV   C+ DA+QDT+YAH+ RQFYRDC I GT
Sbjct: 364 FMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGT 423

Query: 430 IDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSA 489
           +DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  C+    ++    
Sbjct: 424 VDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI 483

Query: 490 PTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKR 549
            T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P TIFY EY NSGPG++   R
Sbjct: 484 QTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNR 543

Query: 550 VKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDS 575
           VKW G + SLT  +A KFTV  F++G +WLPAT V F+S
Sbjct: 544 VKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.5e-130
Identity = 264/577 (45.75%), Postives = 370/577 (64.12%), Query Frame = 0


Query: 10  YGKVDELEQQAF--RRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSSSATNSGP 69
           YGKVDE E      RRKTR+   I+ VS+V+L  +VIGAV G + HK++  +    N+G 
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPAASLKTLCSVTQYPSSCQSSLG---NSNTTDPVFLFKLSLRVATDSLSKLADYS 129
               + + S+K +C VT +   C  +LG   N+++ +P  LF+ ++++    +SK     
Sbjct: 65  ----SISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI--- 124

Query: 130 SKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLS 189
           +  +S+  D      +  C  + +  I+ LN+T++S     NG+  + P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTS---SSNGDVTV-PELVDDLRTWLS 184

Query: 190 TTITDQETCLDAVR-DLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIPIHR 249
           +  T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG ++D +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 250 RLM--GFPEWVSSGDRRLLQEKNV--TAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFII 309
           RL+     E      RRLLQ  ++   A + V+KDG+G + TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 310 YVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGK 369
           YVK+G+Y ENV ++K  WNV++ GDG +++I+SGRLN IDGTPTF TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 370 DMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFI 429
           DMGFINTAGP+KHQAVA    +DL+    C+ +AYQDTLY H+ RQFYR+C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 430 FGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYL 489
           FGN+A V QSC I PR+P+  Q NTITAQG+ D N N+GISI +C+ S   D     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 490 GRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWV 549
           GRPWK FSTTVIM S +  F+   GW  W +G   P TIFYGEY+N+GPG++   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 550 GYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           G R  L+  +A +FTV  F++G  WLPAT V F S L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Tan0006536.1 vs. NCBI nr
Match: XP_038877946.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.8e-301
Identity = 521/576 (90.45%), Postives = 555/576 (96.35%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR IIL+VS+VLLIA+VI AVVGIVIHKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLVSIVLLIALVIAAVVGIVIHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           SAT+S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  SATSSPPPTELTPLASLKTLCSVTQYPSSCQSSLENSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S LNS T DP L+AAI +C SVFEDAI+TLNDTVSSMEVDR+ EKFLSPSRI+DLK
Sbjct: 121 SDYTSNLNSNTEDPKLKAAIEICRSVFEDAIDTLNDTVSSMEVDRHNEKFLSPSRIDDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQ LQTAMANSTEFTSNSLAIVTKIL LL+DF+IP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILRLLADFDIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMGFP+WVSSGDRRLLQE NVTAHVTVSKDG G++TTIK AVAAVPKKSK+RFIIY
Sbjct: 241 IHRKLMGFPDWVSSGDRRLLQENNVTAHVTVSKDGKGNYTTIKDAVAAVPKKSKQRFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRT+TI+SGRLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+SGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVISGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYND+L+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDNLAAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FLQPVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           YRPSLTDA+AGKFTVGTFLNG+DWLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAGKFTVGTFLNGEDWLPATSVKFDTSL 576

[Close]

BLAST of Tan0006536.1 vs. NCBI nr
Match: KAA0053577.1 (pectinesterase 3 [Cucumis melo var. makuwa] >TYK19167.1 pectinesterase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.3e-299
Identity = 514/576 (89.24%), Postives = 550/576 (95.49%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +SVVLLIA++IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NS T DP ++AAI +C SVFEDAI+TLNDT+SSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQELQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDR+LLQE NVTAHVTVSKDG GD+TTIK AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYN+SLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AGKFTVGTFLNG+DWLPAT+V FD++L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Tan0006536.1 vs. NCBI nr
Match: XP_008460354.1 (PREDICTED: pectinesterase 3 [Cucumis melo])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.3e-299
Identity = 514/576 (89.24%), Postives = 550/576 (95.49%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +SVVLLIA++IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NS T DP ++AAI +C SVFEDAI+TLNDT+SSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQ+LQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDRRLLQE NVTAHVTVSKDG GD+TTIK AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYN+SLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AGKFTVGTFLNG+DWLPAT+V FD++L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Tan0006536.1 vs. NCBI nr
Match: XP_023528356.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.2e-297
Identity = 512/576 (88.89%), Postives = 550/576 (95.49%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELE QAFR+KTRRR IIL++SVVLLIA+VIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSVVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T+S PPTEL PAASLKTLCSVTQYPSSCQSSL NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S LNS+T+D  ++AAIG+C SVF+DAI+TLNDTVSSMEVDR+GEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQTAVL+ LQ  MANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LM FPEW+S GDRRLLQE NVTAH TVSKDG GDFTTIK AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTAHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+TRTI+SGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFD YQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FLQP+GWKEWVSGQDPPS+IFYGEY+NSGPGSNV+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGAFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           YRPSLTDA+A KFTV TFLNG+DWLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDTSL 576

[Close]

BLAST of Tan0006536.1 vs. NCBI nr
Match: XP_004144445.1 (pectinesterase 3 [Cucumis sativus] >KGN58475.1 hypothetical protein Csa_001265 [Cucumis sativus])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.7e-297
Identity = 510/576 (88.54%), Postives = 549/576 (95.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +S++LLIA+++GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NSTT DP + AAI +C SVFEDAI+TLNDTVSSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQ+LQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDRRLLQE NVTAHVTVSKDG G +TTI+ AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIM+SEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AG+FTVGTFLNG+DWLPAT+V FD+SL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

[Close]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL
Match: A0A5A7UGG5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 4.0e-299
Identity = 514/576 (89.24%), Postives = 550/576 (95.49%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +SVVLLIA++IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NS T DP ++AAI +C SVFEDAI+TLNDT+SSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQELQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDR+LLQE NVTAHVTVSKDG GD+TTIK AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYN+SLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AGKFTVGTFLNG+DWLPAT+V FD++L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL
Match: A0A1S3CBU9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 4.0e-299
Identity = 514/576 (89.24%), Postives = 550/576 (95.49%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +SVVLLIA++IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NS T DP ++AAI +C SVFEDAI+TLNDT+SSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQ+LQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDRRLLQE NVTAHVTVSKDG GD+TTIK AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYN+SLSAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AGKFTVGTFLNG+DWLPAT+V FD++L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

[Close]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL
Match: A0A0A0L956 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.3e-297
Identity = 510/576 (88.54%), Postives = 549/576 (95.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MD+VKSFKGYGKVDELEQQAFR+KTRRR II+ +S++LLIA+++GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T S PPTELTP ASLKTLCSVTQYPSSCQSSL NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S  NSTT DP + AAI +C SVFEDAI+TLNDTVSSMEVDR+ EKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VLQ+LQTAMANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LMG PEWVSSGDRRLLQE NVTAHVTVSKDG G +TTI+ AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRT+TI+SG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFD YQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIM+SEIG FL PVGWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           Y+PSLTD++AG+FTVGTFLNG+DWLPAT+V FD+SL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

[Close]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL
Match: A0A6J1GVT9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.7e-297
Identity = 510/576 (88.54%), Postives = 549/576 (95.31%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELE QAFR+KTRRR IIL++S+VLLIA+VIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T+S PPTEL PAASLKTLCSVTQYPSSCQSSL NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S LNS+T+D  ++AAIG+C SVF+DAI+TLNDTVSSMEVDR+GEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLNQTAVL+ LQ  MANSTEFTSNSLAIVTKILGLL+DFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LM FPEW+S GDRRLLQE NVT H TVSKDG GDFTTIK AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+TRTI+SGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFD YQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAAVVFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYNDSL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FLQP+GWKEWVSGQDPPS+IFYGEY+NSGPGSNVDKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           YRPSLTDA+A KFTV TFLNG++WLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

[Close]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL
Match: A0A6J1IZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 7.1e-296
Identity = 509/576 (88.37%), Postives = 547/576 (94.97%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MDSVKSFKGYGKVDELE QAFR+KTRRR IIL++S+VLLIA+VIGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T+S PPTEL PAASLKTLCSVTQYPSSCQSSL NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 ADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLK 180
           +DY+S LNS+T+D  ++AAIG+C SVF+DAI+TLNDTVSSMEVDR+GEKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIP 240
           TWLSTTITDQETCLDA+RDLN TAVL+ LQ  MANSTEFTSNSLAIVTKILGLL++FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 241 IHRRLMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIY 300
           IHR+LM FPEWVS GDRRLLQE NVTAH TVSKDG  ++TTIK AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRTRTI+SGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFD YQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYLG 480
           GNAA VFQ+CNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKC+FSAYNDSL+APTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVG 540
           RPWKEFSTTVIMQSEIG FLQPVGWKEWVSGQDPPS+IFYGEY+NSGPGSNV+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           YRPSLTDA+A KFTV TFLNG+DWLPATSVKFDSSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

[Close]

BLAST of Tan0006536.1 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 694.1 bits (1790), Expect = 9.4e-200
Identity = 357/590 (60.51%), Postives = 442/590 (74.92%), Query Frame = 0


Query: 1   MDSVKSFKGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSS 60
           MDSV SFKGYGKVDE +  A ++KTR+R ++L +SVV+LIAV+I AVV  V+HK  + S+
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNEST 60

Query: 61  SATNSGPPTELTPAASLKTLCSVTQYPSSCQSS---LGNSNTTDPVFLFKLSLRVATDSL 120
            +    PP ELTP+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D L
Sbjct: 61  PS----PPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIE 180
             ++D   KL+  T D  +++A+ VC  + EDA++ LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDAVRDLNQ-------TAVLQELQTAMANSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D++ +L Q       + + Q L++AM+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLSDFNIPIHRR----------LMGFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFT 300
           L  LSD  IPIHRR           + F +W     RRLLQ   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIKAAVAAVPKKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDG 360
           T+  AVA VPKKS + F+IYVK G Y ENV++DKSKWNVM+YGDG+ +TIISG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYA 420
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFD +QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCSFSAYNDSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFY 540
           IQ+C+ SA N ++ APTYLGRPWKEFSTTVIM++ IG  ++P GW  WVSG DPP++I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYRNSGPGSNVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSV 571
           GEY+N+GPGS+V +RVKW GY+P ++DA+A KFTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

[Close]

BLAST of Tan0006536.1 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 567.8 bits (1462), Expect = 1.0e-161
Identity = 297/527 (56.36%), Postives = 372/527 (70.59%), Query Frame = 0


Query: 52  IHKRNSSSSSATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLG----NSNTTDPVFLFK 111
           IH+R+   S      P    TP++ L+T+C+VT YP+SC SS+     +  TTDP  LF+
Sbjct: 436 IHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFR 495

Query: 112 LSLRVATDSLSKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSM-EVDRN 171
           LSL+V  D L+ +     KL   T D  L++A+ VC  VF+ A++++NDT+SS+ EV   
Sbjct: 496 LSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISG 555

Query: 172 GEKFLSPSRIEDLKTWLSTTITDQETCLDAVRDLN-QTAVLQELQTAMANSTEFTSNSLA 231
           G+K L+ S I DL TWLS+ +TD  TC D + + N  + + Q+L++AM NSTEFTSNSLA
Sbjct: 556 GKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLA 615

Query: 232 IVTKILGLLSDFNIPIH-RRLM---GFPEWVSSGDRRLLQEKNVTAHVTVSKDGNGDFTT 291
           IV ++L   S   IP+  RRL+    FP WV  G RRLLQ KN+T HVTV+ DG+GD  T
Sbjct: 616 IVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVRT 675

Query: 292 IKAAVAAVPKKSKERFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGT 351
           +  AV  VPKK K  F+IYVK G Y ENV++ K KWNV +YGDGR +TIISG  N +DG 
Sbjct: 676 VNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGV 735

Query: 352 PTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAH 411
            TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFD YQDTLY H
Sbjct: 736 RTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTH 795

Query: 412 SNRQFYRDCDITGTIDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISI 471
           SNRQ+YR+CD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GISI
Sbjct: 796 SNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISI 855

Query: 472 QKCSFSAYNDSLSAPTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQD-PPSTIFY 531
            +C+ S  N +++A TYLGRPWK FS TVIMQS IG F+ P GW  W S  D PP TIFY
Sbjct: 856 HQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFY 915

Query: 532 GEYRNSGPGSNVDKRVKWVGYRPSLTDADAGKFTVGTFLNGQD-WLP 567
            EY+NSGPGS++ KRVKW GY+P  +D +A +FTV  FL G D W+P
Sbjct: 916 REYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

HSP 2 Score: 213.4 bits (542), Expect = 4.8e-55
Identity = 129/253 (50.99%), Postives = 174/253 (68.77%), Query Frame = 0


Query: 1   MDSVKSF-KGYGKVDELEQQAFRRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSS 60
           MD+VKS  KGYGKVDE +  A +RKTR+R   + +SV +L+A++I + V I IH R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 61  SSATNSGPPTELTPAASLKTLCSVTQYPSSCQSSLGN---SNTTDPVFLFKLSLRVATDS 120
              T S  P ELTPAASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 121 LSKLADYSSKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRI 180
           L+ + +   KL   T D  L++A+ VC  + + AI+ +N+TVS+MEV  +G+K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 181 EDLKTWLSTTITDQETCLDAVRDLNQT--AVLQELQTAMANSTEFTSNSLAIVTKILGLL 240
           +DL TWLS  +T   TCLDA+ +++ T  A+  +L++ M NSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 241 SDFNIPIH-RRLM 247
           SDF IPIH RRL+
Sbjct: 241 SDFGIPIHGRRLL 251

[Close]

BLAST of Tan0006536.1 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 493.4 bits (1269), Expect = 2.4e-139
Identity = 278/579 (48.01%), Postives = 379/579 (65.46%), Query Frame = 0


Query: 10  YGKVDELEQQAFR--RKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSSSATNSGP 69
           YG++DE EQ      RKT++R  I+ +S ++L+ +V+GAVVG    + NS      N+G 
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTA-RDNSKKPPTENNGE 63

Query: 70  PTELTPAASLKTLCSVTQYPSSCQSSLG---NSNTTDPVFLFKLSLRVATDSLSKLADYS 129
           P     + S+K LC VT +   C  +LG   N++ + P  LFK +++V    LSK+ D  
Sbjct: 64  PI----SVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 SKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLS 189
           S  N    D    AA+G C+ +   A++ LN+T++S           S    +DL+TWLS
Sbjct: 124 S--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TTITDQETCLDAVRDLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIPIHRR 249
           +  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG ++D      RR
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFRRRR 243

Query: 250 LM--GFPEWVSSG-----DRRLLQEKNV--TAHVTVSKDGNGDFTTIKAAVAAVPKKSKE 309
           L+  G  + V +       RRLL+  ++   A + V+KDG+G + TI  A+A V +K+++
Sbjct: 244 LLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEK 303

Query: 310 RFIIYVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKG 369
             IIYVK+G+Y ENV ++K+KWNV+M GDG+++TI+S  LNFIDGTPTF TATFAV GKG
Sbjct: 304 PTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKG 363

Query: 370 FIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGT 429
           F+ +DMGFINTAGPAKHQAVA    +DLSV   C+ DA+QDT+YAH+ RQFYRDC I GT
Sbjct: 364 FMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGT 423

Query: 430 IDFIFGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSA 489
           +DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  C+    ++    
Sbjct: 424 VDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI 483

Query: 490 PTYLGRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKR 549
            T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P TIFY EY NSGPG++   R
Sbjct: 484 QTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNR 543

Query: 550 VKWVGYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDS 575
           VKW G + SLT  +A KFTV  F++G +WLPAT V F+S
Sbjct: 544 VKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Tan0006536.1 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-131
Identity = 264/577 (45.75%), Postives = 370/577 (64.12%), Query Frame = 0


Query: 10  YGKVDELEQQAF--RRKTRRRFIILVVSVVLLIAVVIGAVVGIVIHKRNSSSSSATNSGP 69
           YGKVDE E      RRKTR+   I+ VS+V+L  +VIGAV G + HK++  +    N+G 
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPAASLKTLCSVTQYPSSCQSSLG---NSNTTDPVFLFKLSLRVATDSLSKLADYS 129
               + + S+K +C VT +   C  +LG   N+++ +P  LF+ ++++    +SK     
Sbjct: 65  ----SISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI--- 124

Query: 130 SKLNSTTTDPNLRAAIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLS 189
           +  +S+  D      +  C  + +  I+ LN+T++S     NG+  + P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTS---SSNGDVTV-PELVDDLRTWLS 184

Query: 190 TTITDQETCLDAVR-DLNQTAVLQELQTAMANSTEFTSNSLAIVTKILGLLSDFNIPIHR 249
           +  T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG ++D +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 250 RLM--GFPEWVSSGDRRLLQEKNV--TAHVTVSKDGNGDFTTIKAAVAAVPKKSKERFII 309
           RL+     E      RRLLQ  ++   A + V+KDG+G + TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 310 YVKEGIYEENVILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGK 369
           YVK+G+Y ENV ++K  WNV++ GDG +++I+SGRLN IDGTPTF TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 370 DMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFI 429
           DMGFINTAGP+KHQAVA    +DL+    C+ +AYQDTLY H+ RQFYR+C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 430 FGNAAVVFQSCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSLSAPTYL 489
           FGN+A V QSC I PR+P+  Q NTITAQG+ D N N+GISI +C+ S   D     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 490 GRPWKEFSTTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWV 549
           GRPWK FSTTVIM S +  F+   GW  W +G   P TIFYGEY+N+GPG++   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 550 GYRPSLTDADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
           G R  L+  +A +FTV  F++G  WLPAT V F S L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Tan0006536.1 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 396.4 bits (1017), Expect = 4.0e-110
Identity = 216/510 (42.35%), Postives = 302/510 (59.22%), Query Frame = 0


Query: 79  TLCSVTQYPSSCQSSLGNSNTTDPVFLFKLSLRVATDSLSKLADYSSKLNSTTTDPNLRA 138
           +LCS  +   S    +       P   F  + +   D+++K     SK +       +  
Sbjct: 20  SLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSN 79

Query: 139 AIGVCLSVFEDAIETLNDTVSSMEVDRNGEKFLSPSRIEDLKTWLSTTITDQETCLDAVR 198
           AI  C+ + + A E L+  +S+ +   NG+   +     DL+TW+S  +++Q+TCLD   
Sbjct: 80  AIVDCVDLLDSAAEELSWIISASQ-SPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 139

Query: 199 DLNQTAVLQELQTAMANSTEFTSNSLAIVT-------KILGLLSDFNIPIHRRLMGFPEW 258
             N   +++++     +    T  +L  +        K   + +      H     FP W
Sbjct: 140 GTN--GIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSW 199

Query: 259 VSSGDRRLLQEKNVT-AHVTVSKDGNGDFTTIKAAVAAVPKKSKERFIIYVKEGIYEENV 318
           V  GDR+LLQ  N+T A   V+ DG G+FTTI  AV A P  S +R++I+VK G+Y ENV
Sbjct: 200 VKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENV 259

Query: 319 ILDKSKWNVMMYGDGRTRTIISGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPA 378
            + K KWN+MM GDG   T+I+G  +FIDG  TF +ATFAV+G+GFI +D+ F NTAGP 
Sbjct: 260 EIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPE 319

Query: 379 KHQAVAFRSGSDLSVMSGCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQSC 438
           KHQAVA RS +DL V   C+   YQDTLYAHS RQF+R+C ITGT+DFIFG+A  VFQSC
Sbjct: 320 KHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSC 379

Query: 439 NIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCSFSAYNDSL----SAPTYLGRPWKEF 498
            I+ +Q LPNQ N+ITAQG+KD N+ +G +IQ  + +A  D L    +  TYLGRPWK +
Sbjct: 380 QIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLY 439

Query: 499 STTVIMQSEIGDFLQPVGWKEWVSGQDPPSTIFYGEYRNSGPGSNVDKRVKWVGYRPSLT 558
           S TV MQ+ + D + PVGW EW +G     T++YGEY NSGPG+++D+RVKW GY    T
Sbjct: 440 SRTVFMQNYMSDAINPVGWLEW-NGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNT 499

Query: 559 DADAGKFTVGTFLNGQDWLPATSVKFDSSL 577
            A+A  FTV   + G  WLP+T + F + L
Sbjct: 500 SAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:

BLAST of Tan0006536.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43111	6.3e-209	63.31	Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1	[more]
Q43867	1.3e-198	60.51	Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1	[more]
Q9LUL8	1.4e-160	56.36	Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF78	3.4e-138	48.01	Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3...	[more]
Q9SG77	1.5e-130	45.75	Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Tan0006536.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877946.1	1.8e-301	90.45	pectinesterase 3 [Benincasa hispida]	[more]
KAA0053577.1	8.3e-299	89.24	pectinesterase 3 [Cucumis melo var. makuwa] >TYK19167.1 pectinesterase 3 [Cucumi...	[more]
XP_008460354.1	8.3e-299	89.24	PREDICTED: pectinesterase 3 [Cucumis melo]	[more]
XP_023528356.1	1.2e-297	88.89	pectinesterase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_004144445.1	2.7e-297	88.54	pectinesterase 3 [Cucumis sativus] >KGN58475.1 hypothetical protein Csa_001265 [...	[more]

BLAST of Tan0006536.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UGG5	4.0e-299	89.24	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970...	[more]
A0A1S3CBU9	4.0e-299	89.24	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1	[more]
A0A0A0L956	1.3e-297	88.54	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1	[more]
A0A6J1GVT9	1.7e-297	88.54	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1	[more]
A0A6J1IZN0	7.1e-296	88.37	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1	[more]

BLAST of Tan0006536.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53840.1	9.4e-200	60.51	pectin methylesterase 1	[more]
AT3G14300.1	1.0e-161	56.36	pectinesterase family protein	[more]
AT5G04960.1	2.4e-139	48.01	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10710.1	1.1e-131	45.75	root hair specific 12	[more]
AT3G43270.1	4.0e-110	42.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 72..226 e-value: 1.2E-33 score: 127.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 59..230 e-value: 3.1E-20 score: 70.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 77..226 e-value: 7.7E-25 score: 88.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 262..576 e-value: 1.1E-113 score: 381.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 73..230 e-value: 1.5E-31 score: 111.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 77..231
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 268..561 e-value: 3.9E-137 score: 455.9
None	No IPR available	PANTHER	PTHR31707:SF219	PECTINESTERASE 1-RELATED	coord: 9..573
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 9..573
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 79..230 e-value: 2.83613E-41 score: 144.503
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 412..421
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 266..573

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0006536	Tan0006536	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006536.1-five_prime_utr	Tan0006536.1-five_prime_utr-LG04:87782835..87782941	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006536.1-exon	Tan0006536.1-exon-LG04:87782835..87783989	exon
Tan0006536.1-exon	Tan0006536.1-exon-LG04:87785413..87786319	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006536.1-cds	Tan0006536.1-cds-LG04:87782942..87783989	CDS
Tan0006536.1-cds	Tan0006536.1-cds-LG04:87785413..87786095	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0006536.1-three_prime_utr	Tan0006536.1-three_prime_utr-LG04:87786096..87786319	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0006536.1	Tan0006536.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity