Tan0005614.1 (mRNA) Snake gourd v1

Overview


Name	Tan0005614.1
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	VOC domain-containing protein
Location	LG06: 4856776 .. 4857680 (+)
Sequence length	812
RNA-Seq Expression	Tan0005614.1
Synteny	Tan0005614.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTACGTCTTCTCTGCAAAAGCTTCCACGTACGGCTGCTCCATCTTCTTCCATGGCAGTTGGCACTCTCCTCCCTTCCCCACGTGTGGCCTATCCGCCGTCACCGTTCCCCCTTTTTAAATCAATCACCATCATCAACACTCTTCTTCACTCCTCTGCTTCGATTCTACGCCGGAACACATGGATGAAGATCGGAAGGCCGTCCAAACAAACAACAACGAATCAGAATCACAAGAAATTAGTGAAAGAGAGAGTACAAGAAAGGATGAAAAAGAGCATCCACTTCCATTAATGTCGCTCAATCACGTCTCCAGAGTGTGCAGAAACGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAATTACGGCGTCGGGATTCACTTGATGCAATCGGAGGATGAAGATAATCATGGAGTTCGTCGAGGGGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCATTTCAGGTAATTAGAATTTAAGAAAGAAAAAAAAGTCGTGAGGATAAATTTTAGTAACGTTTTAGGTTTTCTTTTTGTTGAAATCGAACTGTGTGGCAGTGCGAAGATATGGAAGCGATTGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAAGAGGACATTGGAAGAGGAGAGTACTGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGGTCTGCAACTGCGAGAATCTGAAGGTAGTTCCGGCGGGTTCCTCCGACAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCGTTGGAATCGAATGAACAGAAATAACTCTACGTTTGAGTTGCAGAGAGTCGAGTGCGTTAG

mRNA sequence

CGTACGTCTTCTCTGCAAAAGCTTCCACGTACGGCTGCTCCATCTTCTTCCATGGCAGTTGGCACTCTCCTCCCTTCCCCACGTGTGGCCTATCCGCCGTCACCGTTCCCCCTTTTTAAATCAATCACCATCATCAACACTCTTCTTCACTCCTCTGCTTCGATTCTACGCCGGAACACATGGATGAAGATCGGAAGGCCGTCCAAACAAACAACAACGAATCAGAATCACAAGAAATTAGTGAAAGAGAGAGTACAAGAAAGGATGAAAAAGAGCATCCACTTCCATTAATGTCGCTCAATCACGTCTCCAGAGTGTGCAGAAACGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAATTACGGCGTCGGGATTCACTTGATGCAATCGGAGGATGAAGATAATCATGGAGTTCGTCGAGGGGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCATTTCAGTGCGAAGATATGGAAGCGATTGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAAGAGGACATTGGAAGAGGAGAGTACTGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGGTCTGCAACTGCGAGAATCTGAAGGTAGTTCCGGCGGGTTCCTCCGACAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCGTTGGAATCGAATGAACAGAAATAACTCTACGTTTGAGTTGCAGAGAGTCGAGTGCGTTAG

Coding sequence (CDS)

ATGGATGAAGATCGGAAGGCCGTCCAAACAAACAACAACGAATCAGAATCACAAGAAATTAGTGAAAGAGAGAGTACAAGAAAGGATGAAAAAGAGCATCCACTTCCATTAATGTCGCTCAATCACGTCTCCAGAGTGTGCAGAAACGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAATTACGGCGTCGGGATTCACTTGATGCAATCGGAGGATGAAGATAATCATGGAGTTCGTCGAGGGGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCATTTCAGTGCGAAGATATGGAAGCGATTGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAAGAGGACATTGGAAGAGGAGAGTACTGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGGTCTGCAACTGCGAGAATCTGAAGGTAGTTCCGGCGGGTTCCTCCGACAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCGTTGGAATCGAATGAACAGAAATAA

Protein sequence

MDEDRKAVQTNNNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRIRLPAGRHNPPLESNEQK

Homology

[Close]

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot
Match: Q9CPU0 (Lactoylglutathione lyase OS=Mus musculus OX=10090 GN=Glo1 PE=1 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 5.3e-06
Identity = 37/150 (24.67%), Postives = 67/150 (44.67%), Query Frame = 0


Query: 48  RNVKDSVDFYTKVLGFVLIER---------------------PQSFDFEGAWLFNYGVGI 107
           ++ K S+DFYT+VLG  L+++                     P+    + AW F+    +
Sbjct: 40  KDPKKSLDFYTRVLGLTLLQKLDFPAMKFSLYFLAYEDKNDIPKDKSEKTAWTFSRKATL 99

Query: 108 HLMQ---SEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEE 167
            L     +ED++      G+ D       HI     D+ +  ++ +ELGVK++K+  + +
Sbjct: 100 ELTHNWGTEDDETQSYHNGNSDPRG--FGHIGIAVPDVYSACKRFEELGVKFVKKPDDGK 159

Query: 168 STGESIEQLFFNDPDGFMIEVCNCENLKVV 174
             G +    F  DPDG+ IE+ N   +  +
Sbjct: 160 MKGLA----FIQDPDGYWIEILNPNKIATI 183

[Close]

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot
Match: Q6P7Q4 (Lactoylglutathione lyase OS=Rattus norvegicus OX=10116 GN=Glo1 PE=1 SV=3)

HSP 1 Score: 50.8 bits (120), Expect = 2.0e-05
Identity = 37/150 (24.67%), Postives = 65/150 (43.33%), Query Frame = 0


Query: 48  RNVKDSVDFYTKVLGFVLIER---------------------PQSFDFEGAWLFNYGVGI 107
           ++ K S+DFYT+VLG  L+++                     P+      AW F+    +
Sbjct: 40  KDPKKSLDFYTRVLGLTLLQKLDFPSMKFSLYFLAYEDKNDIPKDKTERTAWAFSRKATL 99

Query: 108 HLMQ---SEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEE 167
            L     +ED++      G+ D       HI     D+    ++ +ELGVK++K+  + +
Sbjct: 100 ELTHNWGTEDDETQSYHNGNSDPRG--FGHIGIAVPDVYEACKRFEELGVKFVKKPDDGK 159

Query: 168 STGESIEQLFFNDPDGFMIEVCNCENLKVV 174
             G +    F  DPDG+ IE+ N   +  +
Sbjct: 160 MKGLA----FVQDPDGYWIEILNPNKMATI 183

[Close]

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot
Match: A4IS40 (Metallothiol transferase FosB OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=fosB PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.6e-05
Identity = 38/129 (29.46%), Postives = 60/129 (46.51%), Query Frame = 0


Query: 40  LNHVSRVCRNVKDSVDFYTKVLGFVLIERPQS---FDFEGAWLFNYGVGIHLMQSEDEDN 99
           +NH++    +++ S+ FY  V G  L+ + ++   FD  G WL      +++ Q      
Sbjct: 6   INHLTFSVSDLEKSIHFYQNVFGAKLLVKGRNLAYFDLNGIWL-----ALNVQQD----- 65

Query: 100 HGVRRGDKDHLDPLDNHISFQC--EDMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFF 159
             + R D  H      HI+F    ED + + EKLKELGV  +     +E    S+   +F
Sbjct: 66  --IPRNDIQH---SYTHIAFSVKEEDFDHVVEKLKELGVNILPGRERDERDKRSV---YF 116

Query: 160 NDPDGFMIE 164
            DPDG   E
Sbjct: 126 TDPDGHKFE 116

[Close]

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot
Match: P16635 (Lactoylglutathione lyase OS=Pseudomonas putida OX=303 GN=gloA PE=1 SV=3)

HSP 1 Score: 49.3 bits (116), Expect = 5.9e-05
Identity = 41/147 (27.89%), Postives = 64/147 (43.54%), Query Frame = 0


Query: 41  NHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDE------ 100
           NH     ++++ S+DFYT+VLGF L+++    + + +  F   V    + ++D+      
Sbjct: 26  NHTMLRVKDIEKSLDFYTRVLGFKLVDKRDFVEAKFSLYFLALVDPATIPADDDARHQWM 85

Query: 101 ----------DNHGVRRGDKD------HLDPLD-NHISFQCEDMEAIEEKLKELGVKYMK 160
                      NHG  R D D      + DP    HI     D+ A  E+ + L V + K
Sbjct: 86  KSIPGVLELTHNHGTER-DADFAYHHGNTDPRGFGHICVSVPDVVAACERFEALQVPFQK 145

Query: 161 RTLEEESTGESIEQLFFNDPDGFMIEV 165
           R     S G      F  DPDG+ +EV
Sbjct: 146 RL----SDGRMNHLAFIKDPDGYWVEV 167

[Close]

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot
Match: Q739M9 (Metallothiol transferase FosB OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=fosB PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 8.5e-04
Identity = 37/132 (28.03%), Postives = 61/132 (46.21%), Query Frame = 0


Query: 37  LMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQS---FDFEGAWLFNYGVGIHLMQSED 96
           L  +NH+     N++DS++FY KVL   L+ R +    F+  G W+      IH+ ++E 
Sbjct: 2   LNGINHLCFSVSNLEDSIEFYEKVLEGELLVRGRKLAYFNICGVWV-ALNEEIHIPRNEI 61

Query: 97  EDNHGVRRGDKDHLDPLDNHISFQCE--DMEAIEEKLKELGVKYMKRTLEEESTGESIEQ 156
             ++               HI+F  E  D E++ ++L+E  V  +K     E      E 
Sbjct: 62  YQSY--------------THIAFSVEQKDFESLLQRLEENDVHILK---GRERDVRDCES 115

Query: 157 LFFNDPDGFMIE 164
           ++F DPDG   E
Sbjct: 122 IYFVDPDGHKFE 115

[Close]

BLAST of Tan0005614.1 vs. NCBI nr
Match: XP_022953340.1 (lactoylglutathione lyase [Cucurbita moschata])

HSP 1 Score: 337.8 bits (865), Expect = 6.3e-89
Identity = 172/199 (86.43%), Postives = 185/199 (92.96%), Query Frame = 0


Query: 4   DRKAVQTN----NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTK 63
           DRKA   N    NN+ ES+EI+ERESTRK+E EHPLPLM+LNHVSRVCR+VKDSVDFYTK
Sbjct: 7   DRKATVINEVQENNDGESEEITERESTRKEE-EHPLPLMALNHVSRVCRSVKDSVDFYTK 66

Query: 64  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCE 123
           VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR+GDKDHLDPLDNHISFQCE
Sbjct: 67  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRQGDKDHLDPLDNHISFQCE 126

Query: 124 DMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSD 183
           DMEA+EE+LKELGVKYM+RTL+EE+ GE IEQLFFNDPDGFMIE+CNCENLKVVPAGSS 
Sbjct: 127 DMEAMEERLKELGVKYMRRTLDEEN-GELIEQLFFNDPDGFMIEICNCENLKVVPAGSSG 186

Query: 184 RIRLPAGRHNPPLESNEQK 199
           RIRLPAGRHNPPLESN QK
Sbjct: 187 RIRLPAGRHNPPLESNGQK 202

[Close]

BLAST of Tan0005614.1 vs. NCBI nr
Match: XP_023548789.1 (lactoylglutathione lyase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 3.1e-88
Identity = 171/199 (85.93%), Postives = 184/199 (92.46%), Query Frame = 0


Query: 4   DRKAVQTN----NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTK 63
           DRKA   N     N+ ES+EI+ERESTRK+E EHPLPLM+LNHVSRVC +VKDSVDFYTK
Sbjct: 7   DRKATVDNEVQEKNDGESEEITERESTRKEE-EHPLPLMALNHVSRVCSSVKDSVDFYTK 66

Query: 64  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCE 123
           VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR+GDKDHLDPLDNHISFQCE
Sbjct: 67  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRQGDKDHLDPLDNHISFQCE 126

Query: 124 DMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSD 183
           DMEA+EE+LKELGVKYM+RTL+EE+ GESIEQLFFNDPDGFMIE+CNCENLKVVPAGSS 
Sbjct: 127 DMEAMEERLKELGVKYMRRTLDEEN-GESIEQLFFNDPDGFMIEICNCENLKVVPAGSSG 186

Query: 184 RIRLPAGRHNPPLESNEQK 199
           RIRLPAGRHNPPLESN QK
Sbjct: 187 RIRLPAGRHNPPLESNGQK 202

[Close]

BLAST of Tan0005614.1 vs. NCBI nr
Match: XP_022991929.1 (lactoylglutathione lyase [Cucurbita maxima])

HSP 1 Score: 335.5 bits (859), Expect = 3.1e-88
Identity = 172/199 (86.43%), Postives = 184/199 (92.46%), Query Frame = 0


Query: 4   DRKAVQTN----NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTK 63
           DRKA   N    NN+ ES+EI+ERESTRK+E EHPLPLM+LNHVSRVCR+VKDSVDFYTK
Sbjct: 7   DRKATVGNEVQENNDGESEEITERESTRKEE-EHPLPLMALNHVSRVCRSVKDSVDFYTK 66

Query: 64  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCE 123
           VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR GDKDHLDPLDNHISFQCE
Sbjct: 67  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRLGDKDHLDPLDNHISFQCE 126

Query: 124 DMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSD 183
           DMEA+EE+LKEL VKYM+RTL+EE+ GESIEQLFFNDPDGFMIE+CNCENLKVVPAGSS 
Sbjct: 127 DMEAMEERLKELEVKYMRRTLDEEN-GESIEQLFFNDPDGFMIEICNCENLKVVPAGSSG 186

Query: 184 RIRLPAGRHNPPLESNEQK 199
           RIRLPAGRHNPPLESN QK
Sbjct: 187 RIRLPAGRHNPPLESNGQK 202

[Close]

BLAST of Tan0005614.1 vs. NCBI nr
Match: KAG6575712.1 (Lactoylglutathione lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 334.7 bits (857), Expect = 5.3e-88
Identity = 169/197 (85.79%), Postives = 184/197 (93.40%), Query Frame = 0


Query: 4   DRKAV---QTNNNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKV 63
           DRKA    +   N+ ES+EI+ERE+TRK+E EHPLPLM+LNHVSRVCR+VKDSVDFYTKV
Sbjct: 7   DRKATVGNEVQENDGESEEITERENTRKEE-EHPLPLMALNHVSRVCRSVKDSVDFYTKV 66

Query: 64  LGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCED 123
           LGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR+GDKDHLDPLDNHISFQCED
Sbjct: 67  LGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRQGDKDHLDPLDNHISFQCED 126

Query: 124 MEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDR 183
           MEA+EE+LKELGVKYM+RTL+EE+ GESIEQLFFNDPDGFMIE+CNCENLKVVPAGSS R
Sbjct: 127 MEAMEERLKELGVKYMRRTLDEEN-GESIEQLFFNDPDGFMIEICNCENLKVVPAGSSGR 186

Query: 184 IRLPAGRHNPPLESNEQ 198
           IRLPAGRHNPPLESN Q
Sbjct: 187 IRLPAGRHNPPLESNGQ 200

[Close]

BLAST of Tan0005614.1 vs. NCBI nr
Match: XP_038899611.1 (lactoylglutathione lyase [Benincasa hispida])

HSP 1 Score: 320.1 bits (819), Expect = 1.4e-83
Identity = 159/197 (80.71%), Postives = 175/197 (88.83%), Query Frame = 0


Query: 4   DRKAVQTNNNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGF 63
           DR     N  +S++ E S+ E+TRK E+EHPLPLM+LNHVSRVCR+VKDSVDFYTKVLGF
Sbjct: 8   DRNPTVDNEIQSKNNEESQ-ETTRKQEEEHPLPLMALNHVSRVCRSVKDSVDFYTKVLGF 67

Query: 64  VLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDK--DHLDPLDNHISFQCEDM 123
           VLIERPQSFDFEGAWLFNYGVGIHLMQSE+EDN GVR G+K  DHLDPLDNHISFQCEDM
Sbjct: 68  VLIERPQSFDFEGAWLFNYGVGIHLMQSEEEDNFGVRGGNKDDDHLDPLDNHISFQCEDM 127

Query: 124 EAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRI 183
           EAIEE+LK LGVKYM+RTLEEE  GESIEQLFFNDPDGFMIE+CNCEN+KVVP GSS RI
Sbjct: 128 EAIEERLKVLGVKYMRRTLEEEENGESIEQLFFNDPDGFMIEICNCENIKVVPTGSSGRI 187

Query: 184 RLPAGRHNPPLESNEQK 199
           +LPAGRHNPPLE N+ K
Sbjct: 188 KLPAGRHNPPLELNDDK 203

[Close]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL
Match: A0A6J1GPD4 (lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111455922 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 172/199 (86.43%), Postives = 185/199 (92.96%), Query Frame = 0


Query: 4   DRKAVQTN----NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTK 63
           DRKA   N    NN+ ES+EI+ERESTRK+E EHPLPLM+LNHVSRVCR+VKDSVDFYTK
Sbjct: 7   DRKATVINEVQENNDGESEEITERESTRKEE-EHPLPLMALNHVSRVCRSVKDSVDFYTK 66

Query: 64  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCE 123
           VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR+GDKDHLDPLDNHISFQCE
Sbjct: 67  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRQGDKDHLDPLDNHISFQCE 126

Query: 124 DMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSD 183
           DMEA+EE+LKELGVKYM+RTL+EE+ GE IEQLFFNDPDGFMIE+CNCENLKVVPAGSS 
Sbjct: 127 DMEAMEERLKELGVKYMRRTLDEEN-GELIEQLFFNDPDGFMIEICNCENLKVVPAGSSG 186

Query: 184 RIRLPAGRHNPPLESNEQK 199
           RIRLPAGRHNPPLESN QK
Sbjct: 187 RIRLPAGRHNPPLESNGQK 202

[Close]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL
Match: A0A6J1JW68 (lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111488425 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.5e-88
Identity = 172/199 (86.43%), Postives = 184/199 (92.46%), Query Frame = 0


Query: 4   DRKAVQTN----NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTK 63
           DRKA   N    NN+ ES+EI+ERESTRK+E EHPLPLM+LNHVSRVCR+VKDSVDFYTK
Sbjct: 7   DRKATVGNEVQENNDGESEEITERESTRKEE-EHPLPLMALNHVSRVCRSVKDSVDFYTK 66

Query: 64  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCE 123
           VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN GVR GDKDHLDPLDNHISFQCE
Sbjct: 67  VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRLGDKDHLDPLDNHISFQCE 126

Query: 124 DMEAIEEKLKELGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSD 183
           DMEA+EE+LKEL VKYM+RTL+EE+ GESIEQLFFNDPDGFMIE+CNCENLKVVPAGSS 
Sbjct: 127 DMEAMEERLKELEVKYMRRTLDEEN-GESIEQLFFNDPDGFMIEICNCENLKVVPAGSSG 186

Query: 184 RIRLPAGRHNPPLESNEQK 199
           RIRLPAGRHNPPLESN QK
Sbjct: 187 RIRLPAGRHNPPLESNGQK 202

[Close]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL
Match: A0A5A7UYM4 (Metallothiol transferase FosB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00230 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.8e-81
Identity = 152/188 (80.85%), Postives = 170/188 (90.43%), Query Frame = 0


Query: 12  NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQS 71
           NNE ++     +E+ RK E EHPLPLM+LNHVSRVC+NVKDSV FYTKVLGFVLIERPQS
Sbjct: 15  NNEIQANNEESQETKRKQE-EHPLPLMALNHVSRVCKNVKDSVHFYTKVLGFVLIERPQS 74

Query: 72  FDFEGAWLFNYGVGIHLMQS-EDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKE 131
           FDFEGAWLFNYGVGIHLMQS ED+D+ GVR  DKDHLDP+DNHISFQCEDMEA+EE+LKE
Sbjct: 75  FDFEGAWLFNYGVGIHLMQSEEDDDSVGVRGSDKDHLDPMDNHISFQCEDMEAMEERLKE 134

Query: 132 LGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRIRLPAGRHNP 191
           LGVKYM+RTLEEE  GE+IEQLFFNDPDGFMIE+CNCENLK+VPAGSS +IRLPAGRHNP
Sbjct: 135 LGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEICNCENLKLVPAGSSGKIRLPAGRHNP 194

Query: 192 PLESNEQK 199
           PLE N++K
Sbjct: 195 PLELNDEK 201

[Close]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL
Match: A0A1S3CFR4 (metallothiol transferase FosB OS=Cucumis melo OX=3656 GN=LOC103499962 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.8e-81
Identity = 151/188 (80.32%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 12  NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQS 71
           NNE ++     +E+ RK E EHPLPLM+LNHVSRVC+NVKDSV FYT VLGFVLIERPQS
Sbjct: 15  NNEIQANNEESQETKRKQE-EHPLPLMALNHVSRVCKNVKDSVHFYTNVLGFVLIERPQS 74

Query: 72  FDFEGAWLFNYGVGIHLMQS-EDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKE 131
           FDFEGAWLFNYGVGIHLMQS ED+D+ GVR  DKDHLDP+DNHISFQCEDMEA+EE+LKE
Sbjct: 75  FDFEGAWLFNYGVGIHLMQSEEDDDSVGVRGSDKDHLDPMDNHISFQCEDMEAMEERLKE 134

Query: 132 LGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRIRLPAGRHNP 191
           LGVKYM+RTLEEE  GE+IEQLFFNDPDGFMIE+CNCENLK+VPAGSS +IRLPAGRHNP
Sbjct: 135 LGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEICNCENLKLVPAGSSGKIRLPAGRHNP 194

Query: 192 PLESNEQK 199
           PLE N++K
Sbjct: 195 PLELNDEK 201

[Close]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL
Match: A0A0A0K9N2 (VOC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420800 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.9e-81
Identity = 152/188 (80.85%), Postives = 171/188 (90.96%), Query Frame = 0


Query: 12  NNESESQEISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQS 71
           NNE ++   +  E+TRK E EHPLPLM+LNHVSRVC+NVKDSV FYTKVLGFVLIERPQS
Sbjct: 77  NNEIQA---NNEETTRKQE-EHPLPLMALNHVSRVCKNVKDSVHFYTKVLGFVLIERPQS 136

Query: 72  FDFEGAWLFNYGVGIHLMQS-EDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKE 131
           FDFEGAWLFNYGVGIHLMQ+ ED+D+ GVR  DKDHLDP+DNHISFQCEDMEA+EE+LKE
Sbjct: 137 FDFEGAWLFNYGVGIHLMQTEEDDDSVGVRGSDKDHLDPMDNHISFQCEDMEAMEERLKE 196

Query: 132 LGVKYMKRTLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRIRLPAGRHNP 191
           LGVKYM+RTLEEE  GE+IEQLFFNDPDGFMIE+CNCENLK+VPAGSS +IRLPAGRHNP
Sbjct: 197 LGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEICNCENLKLVPAGSSGKIRLPAGRHNP 256

Query: 192 PLESNEQK 199
           PLE N++K
Sbjct: 257 PLELNDEK 260

[Close]

BLAST of Tan0005614.1 vs. TAIR 10
Match: AT2G28420.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-61
Identity = 110/173 (63.58%), Postives = 140/173 (80.92%), Query Frame = 0


Query: 20  ISERESTRKDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWL 79
           + E++    DE     PLM+LNHVSR+C++VK S++FYTKVLGFV IERP SFDF+GAWL
Sbjct: 1   MEEKKKKGDDELNSKPPLMALNHVSRLCKDVKKSLEFYTKVLGFVEIERPASFDFDGAWL 60

Query: 80  FNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRT 139
           FNYGVGIHL+Q++D+D       D DHLDP+DNHISFQCEDMEA+E++LKE+ VKY+KRT
Sbjct: 61  FNYGVGIHLVQAKDQDK---LPSDTDHLDPMDNHISFQCEDMEALEKRLKEVKVKYIKRT 120

Query: 140 LEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVPAGSSDRIRLPAGRHNPPL 193
           + +E    +I+QLFFNDPDGFM+E+CNCENL++VP  S+D IRLP  RH PP+
Sbjct: 121 VGDEKDA-AIDQLFFNDPDGFMVEICNCENLELVPCHSADAIRLPEDRHAPPV 169

[Close]

BLAST of Tan0005614.1 vs. TAIR 10
Match: AT1G80160.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-43
Identity = 79/147 (53.74%), Postives = 112/147 (76.19%), Query Frame = 0


Query: 28  KDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 87
           KDE  +PL + SLNH+S +CR+V++S+ FY  VLGF+ I RP SFDF+GAWLF +G+GIH
Sbjct: 2   KDETGNPLHIKSLNHISLLCRSVEESISFYQNVLGFLPIRRPDSFDFDGAWLFGHGIGIH 61

Query: 88  LMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEESTGE 147
           L+QS + +    +   K  ++P DNHISFQCE MEA+E+KLKE+ ++Y++  +EE   G 
Sbjct: 62  LLQSPEPE----KLLKKTEINPKDNHISFQCESMEAVEKKLKEMEIEYVRAVVEE--GGI 121

Query: 148 SIEQLFFNDPDGFMIEVCNCENLKVVP 175
            ++QLFF+DPD FMIE+CNC++L V+P
Sbjct: 122 QVDQLFFHDPDAFMIEICNCDSLPVIP 142

[Close]

BLAST of Tan0005614.1 vs. TAIR 10
Match: AT1G15380.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.0e-41
Identity = 81/161 (50.31%), Postives = 117/161 (72.67%), Query Frame = 0


Query: 28  KDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 87
           K++  +PL L SLNHVS +CR+V +S++FY KVLGF+ I RP+S +FEGAWLF +G+GIH
Sbjct: 2   KEDAGNPLHLTSLNHVSVLCRSVDESMNFYQKVLGFIPIRRPESLNFEGAWLFGHGIGIH 61

Query: 88  LMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEESTGE 147
           L+ + + +    +   K  ++P DNHISFQCE M  +E+KL+E+G+ Y++  +EE   G 
Sbjct: 62  LLCAPEPE----KLPKKTAINPKDNHISFQCESMGVVEKKLEEMGIDYVRALVEE--GGI 121

Query: 148 SIEQLFFNDPDGFMIEVCNCENLKVVP-----AGSSDRIRL 184
            ++QLFF+DPDGFMIE+CNC++L VVP     A S  R++L
Sbjct: 122 QVDQLFFHDPDGFMIEICNCDSLPVVPLVGEMARSCSRVKL 156

[Close]

BLAST of Tan0005614.1 vs. TAIR 10
Match: AT1G15380.2 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.0e-41
Identity = 81/161 (50.31%), Postives = 117/161 (72.67%), Query Frame = 0


Query: 28  KDEKEHPLPLMSLNHVSRVCRNVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 87
           K++  +PL L SLNHVS +CR+V +S++FY KVLGF+ I RP+S +FEGAWLF +G+GIH
Sbjct: 2   KEDAGNPLHLTSLNHVSVLCRSVDESMNFYQKVLGFIPIRRPESLNFEGAWLFGHGIGIH 61

Query: 88  LMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKRTLEEESTGE 147
           L+ + + +    +   K  ++P DNHISFQCE M  +E+KL+E+G+ Y++  +EE   G 
Sbjct: 62  LLCAPEPE----KLPKKTAINPKDNHISFQCESMGVVEKKLEEMGIDYVRALVEE--GGI 121

Query: 148 SIEQLFFNDPDGFMIEVCNCENLKVVP-----AGSSDRIRL 184
            ++QLFF+DPDGFMIE+CNC++L VVP     A S  R++L
Sbjct: 122 QVDQLFFHDPDGFMIEICNCDSLPVVPLVGEMARSCSRVKL 156

[Close]

BLAST of Tan0005614.1 vs. TAIR 10
Match: AT1G80160.2 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 49/96 (51.04%), Postives = 72/96 (75.00%), Query Frame = 0


Query: 79  LFNYGVGIHLMQSEDEDNHGVRRGDKDHLDPLDNHISFQCEDMEAIEEKLKELGVKYMKR 138
           LF +G+GIHL+QS + +    +   K  ++P DNHISFQCE MEA+E+KLKE+ ++Y++ 
Sbjct: 27  LFGHGIGIHLLQSPEPE----KLLKKTEINPKDNHISFQCESMEAVEKKLKEMEIEYVRA 86

Query: 139 TLEEESTGESIEQLFFNDPDGFMIEVCNCENLKVVP 175
            +EE   G  ++QLFF+DPD FMIE+CNC++L V+P
Sbjct: 87  VVEE--GGIQVDQLFFHDPDAFMIEICNCDSLPVIP 116

The following BLAST results are available for this feature:

BLAST of Tan0005614.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9CPU0	5.3e-06	24.67	Lactoylglutathione lyase OS=Mus musculus OX=10090 GN=Glo1 PE=1 SV=3	[more]
Q6P7Q4	2.0e-05	24.67	Lactoylglutathione lyase OS=Rattus norvegicus OX=10116 GN=Glo1 PE=1 SV=3	[more]
A4IS40	2.6e-05	29.46	Metallothiol transferase FosB OS=Geobacillus thermodenitrificans (strain NG80-2)...	[more]
P16635	5.9e-05	27.89	Lactoylglutathione lyase OS=Pseudomonas putida OX=303 GN=gloA PE=1 SV=3	[more]
Q739M9	8.5e-04	28.03	Metallothiol transferase FosB OS=Bacillus cereus (strain ATCC 10987 / NRS 248) O...	[more]

BLAST of Tan0005614.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953340.1	6.3e-89	86.43	lactoylglutathione lyase [Cucurbita moschata]	[more]
XP_023548789.1	3.1e-88	85.93	lactoylglutathione lyase [Cucurbita pepo subsp. pepo]	[more]
XP_022991929.1	3.1e-88	86.43	lactoylglutathione lyase [Cucurbita maxima]	[more]
KAG6575712.1	5.3e-88	85.79	Lactoylglutathione lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038899611.1	1.4e-83	80.71	lactoylglutathione lyase [Benincasa hispida]	[more]

BLAST of Tan0005614.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPD4	3.0e-89	86.43	lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111455922 PE=4 SV=1	[more]
A0A6J1JW68	1.5e-88	86.43	lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111488425 PE=4 SV=1	[more]
A0A5A7UYM4	1.8e-81	80.85	Metallothiol transferase FosB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CFR4	6.8e-81	80.32	metallothiol transferase FosB OS=Cucumis melo OX=3656 GN=LOC103499962 PE=4 SV=1	[more]
A0A0A0K9N2	8.9e-81	80.85	VOC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420800 PE=4 SV...	[more]

BLAST of Tan0005614.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28420.1	1.6e-61	63.58	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G80160.1	1.1e-43	53.74	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G15380.1	1.0e-41	50.31	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G15380.2	1.0e-41	50.31	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G80160.2	1.3e-23	51.04	Lactoylglutathione lyase / glyoxalase I family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 40..164 e-value: 3.5E-11 score: 43.4
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 7..171 e-value: 1.3E-24 score: 89.0
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 33..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..35
None	No IPR available	PANTHER	PTHR46142:SF8	EXPRESSED PROTEIN	coord: 24..196
None	No IPR available	PANTHER	PTHR46142	FAMILY NOT NAMED	coord: 24..196
None	No IPR available	CDD	cd07245	VOC_like	coord: 40..164 e-value: 2.97468E-42 score: 136.294
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 39..167 score: 19.751659

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0005614	Tan0005614	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0005614.1-five_prime_utr	Tan0005614.1-five_prime_utr-LG06:4856776..4856954	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0005614.1-exon	Tan0005614.1-exon-LG06:4856776..4857305	exon
Tan0005614.1-exon	Tan0005614.1-exon-LG06:4857399..4857680	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0005614.1-cds	Tan0005614.1-cds-LG06:4856955..4857305	CDS
Tan0005614.1-cds	Tan0005614.1-cds-LG06:4857399..4857644	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0005614.1-three_prime_utr	Tan0005614.1-three_prime_utr-LG06:4857645..4857680	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0005614.1	Tan0005614.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016740	transferase activity