Tan0004965.2 (mRNA) Snake gourd v1

Overview
NameTan0004965.2
TypemRNA
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPhosphopyruvate hydratase
LocationLG01: 3031818 .. 3036611 (+)
Sequence length1574
RNA-Seq ExpressionTan0004965.2
SyntenyTan0004965.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAATTGTGGAGAAAGAAACGGAAAACGATAGTGCAAGTAAGTTTGAACTCAGAATGGCCACAATCAAGATCGTCAAAGCCCGTCAAATCTTCGATAGTCGTGGAAATCCTACTGTTGAAGTGAGTTTTAACTTCCTCTGAACCAATCCTTTGCATCGAAAATGTCTTACCGGGATGGTTAGTTGTTATTCCACAATCGCCTTGTTTGTTCTTAGCCTAACGTTGGTTGTGTTGTGTTTGTTTTCTGAGAAATGTCGGAATAGAAATAGAGAGTGAATCTATTACTGTTATCGAATGGTGTAACTGGCCGTGTACATGTAGATTCCTTGGCGTGAGTGTTTTACAGAGTTTGGGAAAATTTGAACGTGGAAGTTAATGTGATCGCGATTGCAAATTATTGCTTGGTTTTTTGTTGTTTTATTGATCTGAATTTGGTATCAGGTTGATGTAACTCTTACTGATGGAACACTGGCCAGGGCTGCTGTTCCCAGCGGTGCTTCAACCGGTAAATCGATAGTGTTTGTTTATCTACTTGATTGAAAGTAATCATTCACGGTTTAACTTTTTTCTGGATATAAATAACGAATGTATGGTAGGTGAAGTTTCAACCGGTAGGTCGATAGTGTTTGTTTATCTACTAAACGTAGAGGCTACGAAATTTATTTAAATGTACTTGCCTTGTGTTAAAATTTTGAGGATCAATTTCATCCGTCTTGGAAAGTTATTAAACATTTTCAAGTATTATTTTAAAAAATATATGGAAATCAATGCTGCAAGCTGTAAATATAGATATCTTTGAGGTCATGAATGGTAACCATATACCTTCAAGTTTTCCCAAATGAATCTCGCAAAGTCAAACAGTGGTCCAATAAGATTAGTTGAAAATTTGGTCTGCCTGCACATTCAAGGTGATTAAAAAAAAAATAAAATGAAGGAAGGCAGGGTATCATCTCATCTCTGCATGTATGTATTATATATATCTTGTCACCTGAACTTGTTAGACATCATGATTATCCCATGTACCCATATATATAGTTGCATGAATATCCTGTGCAAAAAAGGTACAAACAGTACGTTTATAGTTCATATTTTTTAACTACCCATATTTTATTGACTGAAGGTATCTATGAAGCTGTTGAGCTCAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTAAGGTTGTATGGTAAAATCGGATATTTAAGGAAAACTTTTCCCCAATATTGGTTGTTCCTTGTTAGGGAGTAACATTTTCCCAAAAATATATACTTTTTCTCTCTTGCATACAAGCATTTTTTTATGGTTCGAGGGAGAAATACTCGGCAGCTTTTTTCCCCCTCTCTTTTGCATACAACACTATTTGCTGTGTGTGTTTTAAAGTTGTCTAATGTGATTGCATGACTTGAAATCCAAGATGCTAATCATTCTATGATTTAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTACTTTAATTGGCAAGGTAAAAGCATTAATTTTATTATCGTCCTTTTCTACTGTCAGTGAATCTAAGTGAGGTTTTTATGTAGTGCATTCAATTTTTCGTGGCATTTCATAGGACCCAAGAGAGCAGACGAAACTTGACAATTTTATGGTGCAGCAACTTGATGGGACCGTTAATGAATGGGGTTGGTGCAAACAAAAGGTATGTTGAACTTTTTTTTTCCACCTAGATTTATTTATAGAAAGGTAGATGTCTCCAATTTTAAGCTAAATTTCCTTCTTTATTCTTGCAGCTGGGAGCAAATGCTATACTGGCAGTCTCACTTGCTATTTGTAAAGCAGGTGCTGCAGTGAACAAGATCCCCCTCTATAAGGTATTGTAGTCTTACGTGACTTTAGAAGAGGGTATTCAATTAATTTTTTTTTTTAATCTTATATTTCACTTGTCTTTCTTTTAATTTCTAGTACATAGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTGCCAGTGCCTGCCTTCAATGTCATTAATGGAGGTTCCCATGCAGGCAATAAACTAGCCATGCAAGTAATATGATTTTGATATATTTTTTCTTTTTGATAGATTAGGTGCTTTACTCTTGGGGTTTACGTATTTTCCTGGTAGTAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTCAAGGAAGCAATGAAAATGGGTGTGGAAGTCTACCATCATCTCAAGGTTGTTTGCAATCCTCTCCTACCCCGGTCTTCTAAATTTTGTAATACAAAGCGGCCGTTGTATCAATACATTTCTGTCTGTTTGCTTTTTAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGCGATGAAGGTGGTTTTGCCCCTAATATTCAGGTCTTGGAGACACTAGCCTTCTGCATATTGTTAAATTCGATGTTGGTGGAATTGTCAAATTGAGTGTGTGGCTGTCGATGTTTTTCTCTTCTTACTTTAGCCCGTTCCTCTTTTCCATTATGGTCGGAAGGGGAGGTTTGTGATTGGATAAGATCGCAGATTCTAATGCAGATAGTTTCCTTCTCCACTATTCTTGTTCACTTATGAATTATGGTTGGTGTTTCTTCATTTCATCAGACACACTACATAGTGGTTATATGGTTTAAAAAAATTTCAGGAGAACAAGGAGGGCCTTGAACTCTTGAAGATAGCTATAGCGAAAGCTGGATACACTGGAAAGGTGCTCTCCTTTTGTCCCTTTTTTTTTACTTTGAATATTGTCTTTTCTAATAGTACCTAGGTCCGAGTCTATTGTATTGATTATACTCTTTCACCACTTCTAGGTTGTAATTGGAATGGATGTTGCCGCTTCAGAATTTTATGATAATAAGGAAAATAATTATGACCTGAATTTTAAGGAAGAGGTATTTGACTCTTAGCAGCTCTTGATTCATGTATTTTTCTTGTTACACATTGGCCTGATGAGAAAATTTGCTGCAGAACAATGATGGATCAGAAAAAATATCTGGAGACAGCTTGAAGAATGTCTACAAATCATTTGTGGCAGATTATCCTATTGTGTCCATTGAAGACCCATTCGATCAGGATGACTGGGAGAATTATGCGAAGCTGACCAGTGAAATTGGCCAGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACTAACCCAAAGGTGTTAAGCTTTTAATCATCAAGTACATCTTGATTTTTTGGATCAGTCTTCTGATATTATCTTGCATCTTGTGACAATTGCAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCTTGTAATTCCCTTCTCTTGAAGGTAATCACTCCACTTTTTCTTCCTCACGAGCATCCATTTTTCCTCTTATTTGTTGAACTTTCTTTAGGCAGCTATCTGCTTGTTACCATTCTTAGTGCTCAAGTAATTTGCAGGAGATTTATAATATTGAGATTCTTATGAATTTTTATCAGGTAAATCAAATTGGTTCTGTGACTGAAAGTATTGAAGCAGTCAAGATGTCTAAGCACGCCGGTTGGGGTGTTATGGCAAGCCATAGAAGGTATGCCACGCTTCATTGCATAGCCATCGGTTCTTTCTTTTTTCCGTAACTGCGTGTCATAATCTCTTCCCCCCAGTCTCCATGTACTACTGATTAGATTTTTCTCTTTTCTGTGCAATTTAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGGCAACAGTAAGTCAACAATCCCTCCATTTGTGAATGCTTTTTAGTTTTACGAACAAATGTGTTTGTAGAATTATTATTACTATATAAGCATGCTCCACTCCCTGCATGAGCCTCATATTGCTTTCGCTGTGTGAGTGATGGGATTTCTGATGTAATTCTAATCCAACATGTAGCCTTCTGCGTAGGTAAAGTAGAAAGCAACTACTTGATCATTTATAAAGCATTGGTTGCAAATCTTTGTTTATGTTTGTGCATACTGAGTTCTTAAGATTATTTTCAATTCCTTAATGGTCATTTATAACATGTTTTCCCAAATTTCGAAACAGGGTCAGATTAAGACCGGTGCACCTTGCAGATCTGAGCGCCTAGCCAAATATAACCAGGTGAGTATTATCAGTTTTAAGTTTTTGCCTCTTGCAAACGAAAAGATTAGCATGATAATAAGCCATTTAGTTCCTTCCATTTTAGTCTATGTAACTTCAAACGCCTAAATTGAGATAGAAGCCTAATAGAAATTTTTTAATTTTTTGGAAAAAGTCAATGAAAGAAACTAACCATGGAAACTAATTTTAAAATTTATTAAAAGTATTGAACATTTAAACTATAAAGTCTGAAATAGAAGTCCCCAAGTAGGGGACCAAAATAGCATTTTAACCTGAATTTCTTTCATCATTATTGGTATAATAACACGACATGCAACTGAATAGGATTTCTATGATAATTTGACCATCCATTGTCTTTTTTTTTTTTTTTTAATCATCTACTGTGATCTATGTTTTTCCAACATCCATAGAGTTTGAATTTGTCTCACGCTCACCTGCAGCTCCTTAGGATAGAAGAGGAGCTCGGACCAGCTGCAATTTATGCTGGATTAAAATTTAGATCACCAGTGGAGCCTTATTGAGATTTTAATGATGAGAAAACAGTCAGCCAGTTCACTAAGTCGATGACTGTGTATGGAACATAAGAAAGTGATGTTCTAGCATGTAAATAAGCATGATCTTCTAGCTGCTTCTTTCTGTCTTTCTTGTTATCTTTTCTTTTTGGAAGAAATCACTTGGAAATCAAGAAATTGAGAAATTGAG

mRNA sequence

GAAAATTGTGGAGAAAGAAACGGAAAACGATAGTGCAAGTAAGTTTGAACTCAGAATGGCCACAATCAAGATCGTCAAAGCCCGTCAAATCTTCGATAGTCGTGGAAATCCTACTGTTGAAGTTGATGTAACTCTTACTGATGGAACACTGGCCAGGGCTGCTGTTCCCAGCGGTGCTTCAACCGGTATCTATGAAGCTGTTGAGCTCAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTAAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTACTTTAATTGGCAAGGACCCAAGAGAGCAGACGAAACTTGACAATTTTATGGTGCAGCAACTTGATGGGACCGTTAATGAATGGGGTTGGTGCAAACAAAAGCTGGGAGCAAATGCTATACTGGCAGTCTCACTTGCTATTTGTAAAGCAGGTGCTGCAGTGAACAAGATCCCCCTCTATAAGTACATAGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTGCCAGTGCCTGCCTTCAATGTCATTAATGGAGGTTCCCATGCAGGCAATAAACTAGCCATGCAAGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTCAAGGAAGCAATGAAAATGGGTGTGGAAGTCTACCATCATCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGCGATGAAGGTGGTTTTGCCCCTAATATTCAGGAGAACAAGGAGGGCCTTGAACTCTTGAAGATAGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTTGCCGCTTCAGAATTTTATGATAATAAGGAAAATAATTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATATCTGGAGACAGCTTGAAGAATGTCTACAAATCATTTGTGGCAGATTATCCTATTGTGTCCATTGAAGACCCATTCGATCAGGATGACTGGGAGAATTATGCGAAGCTGACCAGTGAAATTGGCCAGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACTAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCTTGTAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCTGTGACTGAAAGTATTGAAGCAGTCAAGATGTCTAAGCACGCCGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGGCAACAGGTCAGATTAAGACCGGTGCACCTTGCAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAGGAGCTCGGACCAGCTGCAATTTATGCTGGATTAAAATTTAGATCACCAGTGGAGCCTTATTGAGATTTTAATGATGAGAAAACAGTCAGCCAGTTCACTAAGTCGATGACTGTGTATGGAACATAAGAAAGTGATGTTCTAGCATGTAAATAAGCATGATCTTCTAGCTGCTTCTTTCTGTCTTTCTTGTTATCTTTTCTTTTTGGAAGAAATCACTTGGAAATCAAGAAATTGAGAAATTGAG

Coding sequence (CDS)

ATGGCCACAATCAAGATCGTCAAAGCCCGTCAAATCTTCGATAGTCGTGGAAATCCTACTGTTGAAGTTGATGTAACTCTTACTGATGGAACACTGGCCAGGGCTGCTGTTCCCAGCGGTGCTTCAACCGGTATCTATGAAGCTGTTGAGCTCAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTAAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTACTTTAATTGGCAAGGACCCAAGAGAGCAGACGAAACTTGACAATTTTATGGTGCAGCAACTTGATGGGACCGTTAATGAATGGGGTTGGTGCAAACAAAAGCTGGGAGCAAATGCTATACTGGCAGTCTCACTTGCTATTTGTAAAGCAGGTGCTGCAGTGAACAAGATCCCCCTCTATAAGTACATAGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTGCCAGTGCCTGCCTTCAATGTCATTAATGGAGGTTCCCATGCAGGCAATAAACTAGCCATGCAAGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTCAAGGAAGCAATGAAAATGGGTGTGGAAGTCTACCATCATCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGCGATGAAGGTGGTTTTGCCCCTAATATTCAGGAGAACAAGGAGGGCCTTGAACTCTTGAAGATAGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTTGCCGCTTCAGAATTTTATGATAATAAGGAAAATAATTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATATCTGGAGACAGCTTGAAGAATGTCTACAAATCATTTGTGGCAGATTATCCTATTGTGTCCATTGAAGACCCATTCGATCAGGATGACTGGGAGAATTATGCGAAGCTGACCAGTGAAATTGGCCAGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACTAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCTTGTAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCTGTGACTGAAAGTATTGAAGCAGTCAAGATGTCTAAGCACGCCGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGGCAACAGGTCAGATTAAGACCGGTGCACCTTGCAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAGGAGCTCGGACCAGCTGCAATTTATGCTGGATTAAAATTTAGATCACCAGTGGAGCCTTATTGA

Protein sequence

MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGKGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGYTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIEDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGPAAIYAGLKFRSPVEPY
Homology
BLAST of Tan0004965.2 vs. ExPASy Swiss-Prot
Match: Q42971 (Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2)

HSP 1 Score: 781.2 bits (2016), Expect = 6.3e-225
Identity = 389/444 (87.61%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 2   ATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGK 61
           ATI  VKARQIFDSRGNPTVEVDV  +DGT ARAAVPSGASTG+YEA+ELRDGGSDYLGK
Sbjct: 3   ATIVSVKARQIFDSRGNPTVEVDVCCSDGTFARAAVPSGASTGVYEALELRDGGSDYLGK 62

Query: 62  GVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV KAV+NVNS+I P LIGKDP  Q +LDNFMVQQLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVDNVNSVIAPALIGKDPTSQAELDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           AICKAGA + KIPLY++IA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AICKAGAIIKKIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGY 241
           SFKEAMKMGVEVYH+LK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIE 301
           TGKVVIGMDVAASEFY++K+  YDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYNDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDWE+YAK+T+EIG+QVQIVGDDLLVTNP RV KAI+EK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWEHYAKMTAEIGEQVQIVGDDLLVTNPTRVAKAIQEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVKMSK AGWGVM SHRSGETEDTFIADL+VGLATGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLATGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGLKFRSPVEPY 446
           RIEEELG AA+YAG KFR+PVEPY
Sbjct: 423 RIEEELGAAAVYAGAKFRAPVEPY 446

BLAST of Tan0004965.2 vs. ExPASy Swiss-Prot
Match: P42896 (Enolase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 1.8e-224
Identity = 392/443 (88.49%), Postives = 417/443 (94.13%), Query Frame = 0

Query: 3   TIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGKG 62
           TI  V+ARQIFDSRGNPTVE D+ L+DG LARAAVPSGASTGIYEA+ELRDGGSDYLGKG
Sbjct: 4   TIVSVRARQIFDSRGNPTVEADIKLSDGHLARAAVPSGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 122
           V KAVENVNSIIGP LIGKDP EQT LDNFMVQ+LDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VSKAVENVNSIIGPALIGKDPTEQTALDNFMVQELDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 ICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           +CKAGA V  IPLYK+IA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 LCKAGAHVKGIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGYT 242
           FKEAMKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIED 302
           GKVVIGMDVAASEFY   +  YDLNFKEENNDGS+KISG++LK++YKSF ++YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFY-GSDKTYDLNFKEENNDGSQKISGEALKDLYKSFASEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDWE+Y+KLTSEIG++VQIVGDDLLVTNPKRVEKAI+EKACN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWEHYSKLTSEIGEKVQIVGDDLLVTNPKRVEKAIQEKACNALLLKVNQIGSVTE 363

Query: 363 SIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 422
           SIEAV+MSK AGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVRMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGLKFRSPVEPY 446
           IEEELG  A+YAG KFR+PVEPY
Sbjct: 424 IEEELGAEAVYAGAKFRTPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy Swiss-Prot
Match: P42895 (Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 7.0e-224
Identity = 386/444 (86.94%), Postives = 416/444 (93.69%), Query Frame = 0

Query: 2   ATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGK 61
           ATI+ VKARQIFDSRGNPTVEVDV  +DGT ARAAVPSGASTG+YEA+ELRDGGS YLGK
Sbjct: 3   ATIQSVKARQIFDSRGNPTVEVDVFCSDGTFARAAVPSGASTGVYEALELRDGGSYYLGK 62

Query: 62  GVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV KAV NVNS+IGP LIGKDP  QT++DNFMVQQLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVNNVNSVIGPALIGKDPTAQTEIDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           A+CKAGA++ +IPLY++IA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AVCKAGASIKRIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGY 241
           SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIE 301
           TGKVVIGMDVAASEFY +K+  YDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYSDKDQTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDW +YAK+T EIG+QVQIVGDDLLVTNP RV KAIKEK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWVHYAKMTEEIGEQVQIVGDDLLVTNPTRVAKAIKEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVKMSK AGWGVM SHRSGETEDTFIADL+VGL+TGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGLKFRSPVEPY 446
           RIEEELG  A+YAG KFR+PVEPY
Sbjct: 423 RIEEELGAIAVYAGAKFRAPVEPY 446

BLAST of Tan0004965.2 vs. ExPASy Swiss-Prot
Match: Q9LEJ0 (Enolase 1 OS=Hevea brasiliensis OX=3981 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 1.7e-222
Identity = 390/443 (88.04%), Postives = 413/443 (93.23%), Query Frame = 0

Query: 3   TIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGKG 62
           TI  V+ARQIFDSRGNPTVE DV L+DG LARAAVPSGASTGIYEA+ELRDGGSDYLGKG
Sbjct: 4   TIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPSGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 122
           V KAVENVN IIGP L+GKDP +Q  +DNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 ICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           +CKAGA V  IPLY++IA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 VCKAGAHVKGIPLYEHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGYT 242
           FKEAMKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIED 302
           GKVVIGMDVAASEFY   +  YDLNFKEENN+GS+KISG++LK++YKSFVA+YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFY-GSDQTYDLNFKEENNNGSQKISGEALKDLYKSFVAEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDW +YAKLTSEIG++VQIVGDDLLVTNPKRVEKAIKEKACN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWAHYAKLTSEIGEKVQIVGDDLLVTNPKRVEKAIKEKACNALLLKVNQIGSVTE 363

Query: 363 SIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 422
           SIEAVKMSK AGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGLKFRSPVEPY 446
           IEEELG  A+YAG  FR PVEPY
Sbjct: 424 IEEELGSEAVYAGANFRKPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy Swiss-Prot
Match: Q9LEI9 (Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 2.9e-222
Identity = 390/443 (88.04%), Postives = 411/443 (92.78%), Query Frame = 0

Query: 3   TIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLGKG 62
           TI  V+ARQIFDSRGNPTVE DV L+DG LARAAVP GASTGIYEA+ELRDGGSDYLGKG
Sbjct: 4   TIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPRGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 122
           V KAVENVN IIGP L+GKDP +Q  +DNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 ICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           +CKAGA V  IPLYK++A LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 VCKAGAHVKGIPLYKHVANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGYT 242
           FKEAMKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIED 302
           GKVVIGMDVAASEFY   +  YDLNFKEENN+GS+KISGD LK++YKSFV +YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFY-GSDKTYDLNFKEENNNGSQKISGDVLKDLYKSFVTEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDWE+YAKLTSEIG +VQIVGDDLLVTNPKRVEKAIKEKACN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWEHYAKLTSEIGVKVQIVGDDLLVTNPKRVEKAIKEKACNALLLKVNQIGSVTE 363

Query: 363 SIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 422
           SIEAVKMSK AGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGLKFRSPVEPY 446
           IEEELG  A+YAG  FR+PVEPY
Sbjct: 424 IEEELGAEAVYAGANFRTPVEPY 445

BLAST of Tan0004965.2 vs. NCBI nr
Match: KAG6576780.1 (Enolase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014813.1 Enolase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850.9 bits (2197), Expect = 5.0e-243
Identity = 428/445 (96.18%), Postives = 439/445 (98.65%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATI+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. NCBI nr
Match: XP_023552596.1 (enolase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.4e-242
Identity = 428/445 (96.18%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MA I+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. NCBI nr
Match: XP_022922793.1 (enolase-like [Cucurbita moschata])

HSP 1 Score: 849.0 bits (2192), Expect = 1.9e-242
Identity = 427/445 (95.96%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MA I+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. NCBI nr
Match: XP_022141329.1 (enolase-like [Momordica charantia])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 427/445 (95.96%), Postives = 439/445 (98.65%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATIKIVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MATIKIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLKAV+NVNSIIGP LIGKDPREQT+LDNFMVQ+LDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVDNVNSIIGPALIGKDPREQTELDNFMVQKLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYD+K+  YDLNFKEE+NDGSEKISGDSLKNVYKSFV DYP+VSI
Sbjct: 241 YTGKVVIGMDVAASEFYDSKDKTYDLNFKEEHNDGSEKISGDSLKNVYKSFVTDYPVVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIGQQVQIVGDDLLVTNPKRV+KAIKEK CNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGQQVQIVGDDLLVTNPKRVDKAIKEKTCNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. NCBI nr
Match: XP_022984196.1 (enolase 2-like [Cucurbita maxima])

HSP 1 Score: 847.0 bits (2187), Expect = 7.2e-242
Identity = 428/445 (96.18%), Postives = 437/445 (98.20%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATI+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGS YLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSAYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVWQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy TrEMBL
Match: A0A6J1E7U0 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111430677 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 9.1e-243
Identity = 427/445 (95.96%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MA I+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy TrEMBL
Match: A0A6J1CIC4 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111011749 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.6e-242
Identity = 427/445 (95.96%), Postives = 439/445 (98.65%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATIKIVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MATIKIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLKAV+NVNSIIGP LIGKDPREQT+LDNFMVQ+LDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVDNVNSIIGPALIGKDPREQTELDNFMVQKLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYD+K+  YDLNFKEE+NDGSEKISGDSLKNVYKSFV DYP+VSI
Sbjct: 241 YTGKVVIGMDVAASEFYDSKDKTYDLNFKEEHNDGSEKISGDSLKNVYKSFVTDYPVVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIGQQVQIVGDDLLVTNPKRV+KAIKEK CNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGQQVQIVGDDLLVTNPKRVDKAIKEKTCNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy TrEMBL
Match: A0A6J1J4K4 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482586 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.5e-242
Identity = 428/445 (96.18%), Postives = 437/445 (98.20%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATI+IVKARQIFDSRGNPTVEVD+TLTDGTLARAAVPSGASTGIYEA+ELRDGGS YLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSAYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVNSIIGP LIGKDPREQTKLDNFMV QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVWQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGAAVNKIPLYK+IAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDNK   YDLNFKEENNDGSEKISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIG+QVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELGPAAIYAG KFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy TrEMBL
Match: A0A200QB48 (Phosphopyruvate hydratase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.7e-236
Identity = 418/445 (93.93%), Postives = 431/445 (96.85%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATIK VKARQIFDSRGNPTVEVDVTL+DGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MATIKAVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLKAVENVN IIGP LIGKDP +Q +LDNFMVQQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNGIIGPALIGKDPTKQVELDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAICKAGA+VNKIPLYK+IA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGASVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYD+K+  YDLNFKEENNDGS+KISGDSLKNVYKSFVADYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVADYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIGQQVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKMSK AGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKQAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELG AAIYAG KFR+PVEPY
Sbjct: 421 LRIEEELGSAAIYAGAKFRTPVEPY 445

BLAST of Tan0004965.2 vs. ExPASy TrEMBL
Match: A0A5B7BVG7 (Phosphopyruvate hydratase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_041547 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 1.1e-235
Identity = 415/445 (93.26%), Postives = 428/445 (96.18%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATIK VKARQIFDSRGNPTVEVDV L+DGTLARAAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 29  MATIKAVKARQIFDSRGNPTVEVDVILSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 88

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLKAV NVNSIIGP LIGKDP EQTK+DNFMVQQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 89  KGVLKAVGNVNSIIGPALIGKDPTEQTKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 148

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA VNKIPLYK+IA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 149 LAVCKAGAIVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 208

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           +SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 209 ASFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 268

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFYDN +  YDLNFKEENNDGS+KISGDSLKNVYKSFV DYPIVSI
Sbjct: 269 YTGKVVIGMDVAASEFYDNNDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVTDYPIVSI 328

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLT EIG+QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 329 EDPFDQDDWEHYAKLTCEIGEQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 388

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL
Sbjct: 389 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 448

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELG AA+YAG KFR+PVEPY
Sbjct: 449 LRIEEELGSAAVYAGAKFRAPVEPY 473

BLAST of Tan0004965.2 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 758.1 bits (1956), Expect = 4.1e-219
Identity = 379/445 (85.17%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSDYLG 60
           MATI +VKARQIFDSRGNPTVEVD+  ++G    AAVPSGASTGIYEA+ELRDGGSDYLG
Sbjct: 1   MATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120
           KGV KAV NVN+IIGP LIGKDP +QT +DNFMV +LDGT NEWGWCKQKLGANAILAVS
Sbjct: 61  KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120

Query: 121 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA V+ IPLYK+IA LAGN K+VLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240
           +SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAG
Sbjct: 181 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 300
           YTGKVVIGMDVAASEFY +++  YDLNFKEENN+GS+KISGD+LK++YKSFVA+YPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFY-SEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+T+E G +VQIVGDDLLVTNPKRV KAI EK+CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSV 360

Query: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKMSK AGWGVM SHRSGETEDTFIADL+VGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGLKFRSPVEPY 446
           LRIEEELG  AIYAG+ FR PVEPY
Sbjct: 421 LRIEEELGSEAIYAGVNFRKPVEPY 444

BLAST of Tan0004965.2 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 575.5 bits (1482), Expect = 3.8e-164
Identity = 301/439 (68.56%), Postives = 357/439 (81.32%), Query Frame = 0

Query: 4   IKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGG-SDYLGKG 63
           +K VKARQI DSRGNPTVEVD+   D  L R+AVPSGASTGIYEA+ELRDG  S Y GKG
Sbjct: 51  VKGVKARQIIDSRGNPTVEVDLITDD--LYRSAVPSGASTGIYEALELRDGDKSVYGGKG 110

Query: 64  VLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123
           VL+A++N+N ++ P LIG D R Q  +D  M+ +LDGT N     K KLGANAIL VSL+
Sbjct: 111 VLQAIKNINELVAPKLIGVDVRNQADVDALML-ELDGTPN-----KSKLGANAILGVSLS 170

Query: 124 ICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183
           +C+AGA    +PLYK+I + +G K+LV+PVPAFNVINGGSHAGN LAMQEFMILPVGA+S
Sbjct: 171 VCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATS 230

Query: 184 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAGYT 243
           F EA +MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AI KAGYT
Sbjct: 231 FSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYT 290

Query: 244 GKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSIED 303
           GK+ IGMDVAASEF+  K+  YDLNFK++ NDG+  +S +SL ++Y+ F+ D+PIVSIED
Sbjct: 291 GKIKIGMDVAASEFF-MKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIED 350

Query: 304 PFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 363
           PFDQDDW ++A L S +   +Q+VGDDLLVTNPKR+ +AIK+++CN+LLLKVNQIG+VTE
Sbjct: 351 PFDQDDWSSWASLQSSV--DIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTE 410

Query: 364 SIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423
           SI+A   SK AGWGVM SHRSGETED FIADLSVGLA+GQIKTGAPCRSERL+KYNQLLR
Sbjct: 411 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLR 470

Query: 424 IEEELGPAAIYAGLKFRSP 442
           IEEELG    YAG  FRSP
Sbjct: 471 IEEELGNVR-YAGEAFRSP 477

BLAST of Tan0004965.2 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-130
Identity = 251/435 (57.70%), Postives = 312/435 (71.72%), Query Frame = 0

Query: 2   ATIKIVKARQIFDSRGNPTVEVDVTLTDGTLARAAVPSGASTGIYEAVELRDGGSD-YLG 61
           + I  VKARQI DSRG PTVEVD+    G   RA+VPSG S+G YEA+ELRDG    YLG
Sbjct: 45  SVITKVKARQILDSRGIPTVEVDLHTNKGVF-RASVPSGDSSGTYEAIELRDGDKGMYLG 104

Query: 62  KGVLKAVENVNSIIGPTLIGKDPREQTKLDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 121
             V KAV+N+N  I   LIG DP+ Q ++D  M+  LD T       K +LGANAILAVS
Sbjct: 105 NSVAKAVKNINEKISEALIGMDPKLQGQIDQAMI-DLDKTEK-----KSELGANAILAVS 164

Query: 122 LAICKAGAAVNKIPLYKYIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 181
           +A CKAGAA  ++PL K+++ L+G   +VLPVPAF V++GG HA N  A+QE MILP+GA
Sbjct: 165 IAACKAGAAEKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGA 224

Query: 182 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 241
           S F+EA++ G E YHHLKAVI +K G    NVG++GG AP+I   KEGLEL+K AI + G
Sbjct: 225 SRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTG 284

Query: 242 YTGKVVIGMDVAASEFYDNKENNYDLNFKEENNDGSEKISGDSLKNVYKSFVADYPIVSI 301
           Y  K+ I +D+AA+ F       YDL+ K  N  G    S + + ++YK    DYPIVSI
Sbjct: 285 YNDKIKIAIDIAATNFC--LGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSI 344

Query: 302 EDPFDQDDWENYAKLTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 361
           EDPFD++DWE + K  S +G   Q+VGDDLL++N KRVE+AI+E +CN+LLLKVNQIG+V
Sbjct: 345 EDPFDKEDWE-HTKYFSSLG-ICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTV 404

Query: 362 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 421
           TE+IE VKM++ A WGV+ SHR GETED+FI+DLSVGLATG IK GAPCR ER  KYNQL
Sbjct: 405 TEAIEVVKMARDAQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQL 464

Query: 422 LRIEEELGPAAIYAG 436
           LRIEEELG  A+YAG
Sbjct: 465 LRIEEELGDQAVYAG 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q429716.3e-22587.61Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2[more]
P428961.8e-22488.49Enolase OS=Ricinus communis OX=3988 PE=2 SV=1[more]
P428957.0e-22486.94Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1[more]
Q9LEJ01.7e-22288.04Enolase 1 OS=Hevea brasiliensis OX=3981 GN=ENO1 PE=1 SV=1[more]
Q9LEI92.9e-22288.04Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6576780.15.0e-24396.18Enolase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014813.1 Enolase, ... [more]
XP_023552596.11.4e-24296.18enolase-like [Cucurbita pepo subsp. pepo][more]
XP_022922793.11.9e-24295.96enolase-like [Cucurbita moschata][more]
XP_022141329.13.2e-24295.96enolase-like [Momordica charantia][more]
XP_022984196.17.2e-24296.18enolase 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E7U09.1e-24395.96Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111430677 PE=3 SV=... [more]
A0A6J1CIC41.6e-24295.96Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111011749 PE=3 SV... [more]
A0A6J1J4K43.5e-24296.18Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482586 PE=3 SV=1[more]
A0A200QB481.7e-23693.93Phosphopyruvate hydratase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=... [more]
A0A5B7BVG71.1e-23593.26Phosphopyruvate hydratase (Fragment) OS=Davidia involucrata OX=16924 GN=Din_0415... [more]
Match NameE-valueIdentityDescription
AT2G36530.14.1e-21985.17Enolase [more]
AT1G74030.13.8e-16468.56enolase 1 [more]
AT2G29560.12.1e-13057.70cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 379..396
score: 83.84
coord: 327..338
score: 75.51
coord: 350..364
score: 80.2
coord: 37..51
score: 85.25
coord: 112..128
score: 79.68
coord: 169..182
score: 77.06
IPR000941EnolasePIRSFPIRSF001400Enolasecoord: 1..440
e-value: 3.8E-191
score: 633.3
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 4..438
e-value: 9.9E-176
score: 582.5
IPR000941EnolaseSFLDSFLDF00002enolasecoord: 2..427
e-value: 0.0
score: 643.4
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 1..445
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 2..427
score: 44.529926
IPR000941EnolaseCDDcd03313enolasecoord: 7..426
e-value: 0.0
score: 753.162
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 147..441
e-value: 1.6E-203
score: 692.1
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 148..440
e-value: 1.7E-157
score: 523.2
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 4..139
e-value: 1.1E-82
score: 290.7
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 4..139
e-value: 2.3E-53
score: 179.9
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 133..444
e-value: 3.7E-164
score: 547.2
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 147..440
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 1..131
e-value: 3.0E-59
score: 201.0
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 4..143
NoneNo IPR availableSFLDSFLDS00001Enolasecoord: 2..427
e-value: 0.0
score: 643.4
NoneNo IPR availablePANTHERPTHR11902:SF41ENOLASEcoord: 1..445
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 350..363

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Tan0004965Tan0004965gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0004965.2-five_prime_utrTan0004965.2-five_prime_utr-LG01:3031818..3031872five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0004965.2-exonTan0004965.2-exon-LG01:3031818..3031938exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3032260..3032323exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3032939..3033003exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3033263..3033310exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3033407..3033493exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3033585..3033665exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3033757..3033858exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3033940..3034020exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3034113..3034187exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3034465..3034527exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3034631..3034711exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3034788..3034982exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3035066..3035116exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3035269..3035357exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3035481..3035532exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3035853..3035909exon
Tan0004965.2-exonTan0004965.2-exon-LG01:3036350..3036611exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0004965.2-cdsTan0004965.2-cds-LG01:3031873..3031938CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3032260..3032323CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3032939..3033003CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3033263..3033310CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3033407..3033493CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3033585..3033665CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3033757..3033858CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3033940..3034020CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3034113..3034187CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3034465..3034527CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3034631..3034711CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3034788..3034982CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3035066..3035116CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3035269..3035357CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3035481..3035532CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3035853..3035909CDS
Tan0004965.2-cdsTan0004965.2-cds-LG01:3036350..3036430CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0004965.2-three_prime_utrTan0004965.2-three_prime_utr-LG01:3036431..3036611three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Tan0004965.2Tan0004965.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0042254 ribosome biogenesis
cellular_component GO:0000015 phosphopyruvate hydratase complex
cellular_component GO:0005730 nucleolus
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity