Spg035494.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg035494.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Glucan endo-1,3-beta-glucosidase
Location	scaffold3: 2306984 .. 2308675 (+)
Sequence length	1479
RNA-Seq Expression	Spg035494.1
Synteny	Spg035494.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATTTCCAATTCACTCTTCTCACTCTCCTCCATCTTCATCACCTCCCATGGCTTCCTCTTTAGCTCCCTCCCACCTTCTTCTTCTTTACACTTCAACTCCTACTTTCCTTCTTCTTCTTCTTCTTCTTCTCCCCACTCTCATCCAAATCCAAGGTAATTACATCACATCACATCACATATCATTTCCAGACATTATTATGCGCTTTCTTTTCATTATCATCTCAGATATCTATTTCAATTATCATAGTTTGTTAAATTACCTTTGTTCAGGAGCCCAAGGTGGATCCATCGGAGTCAATTACGGCACCATCGCTGACAATCTCCCTCCGCCGTCCAAGGTTGCAGAGTTCCTCTTGGATTCCACCATAATCGACCGCATCAGACTCTTCGATGCCAATTCCGACATCTTACGAGCTTTCGCTCACACCGGCATCTCCGTAACCGTCACCGTCCCCAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCCGAAGAGTGGATCAAATTCAACGTCCAACCCTACGTCCCTGCCACCAACATAATCCGCATTCTAGTCGGCAACGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCGAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCGCCGGAGCCTCCTTGGAGCGGCGGATTCAAGTCTCGACACCGCACTCTCTCGGCATCTTGTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGTTACGACACGCACGTAATGAAGCCGATGCTGAGCTTCCTGAGAGAAACAAACTCGCCGTTGATGGTGAATCCGTATCCATTCTTCGGCTCCTCCGCGGAAACCCTAGACTACGCGCTCTTCCGGGCGAACCCCGGCGTGTTCGATTCAGACTCGGGGGTTTTATACACCAACATGTTGGACGCTCAATTGGACGCTGTTTACTCCGCCATGAAGAGTTTGGGCTTTGAGGATCTGGACATCGTTATTGCGGAAACGGGCTGGCCCTCGAAGGGTGACCCGGCCCAGTTCGGCGTCGGCCCAAAAGAAGCCGCCGACTACAACGGGAATCTCATGCGACATGTGGTATCGGGCGTGGGGACGCCGCTGATGCCGAACCGGACGTTCGAGACGTACATTTTCGCTCTGTTCAACGAGGATCTGAAGCCGGGCCCGGTTGGTGAGAGGAACTTTGGGCTATTTGAACCGGATCTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGTAGCCGCCACAGCCCGAACACATCCTAAAACGTTTTTGATAATCATGATCGTTACATGTTGGTTGATTGGTTTGTTGTCGACTTGGATCTAACAGGCTCAGTCGTCTAATCCCAGAGCCCATCACGGCCCAAAAAGTCCAAGTCTGGGCCCAAGCCCAAGCCCAAGCCCAGGCCCAGTGACTGATGGTAAAAGATGGTGCCTGCCCAAATCTGGAGCCGACTCGGAGGCCTTGCAGAGAAACATTGATTACGTTTGTGGGCTGGGCTTGGATTGTGGGCCGATTCAAGAAAATGGGCCGTGCTTTGTTCCCAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCGTACTTTCAATCCACCGAAGGGAACGATTCTGATTGCGATTTTGAGCACACGGGCGCCCTAACCACTGTGGATCCAAGTAAGTACTAA

mRNA sequence

ATGCCATTTCCAATTCACTCTTCTCACTCTCCTCCATCTTCATCACCTCCCATGGCTTCCTCTTTAGCTCCCTCCCACCTTCTTCTTCTTTACACTTCAACTCCTACTTTCCTTCTTCTTCTTCTTCTTCTTCTCCCCACTCTCATCCAAATCCAAGGAGCCCAAGGTGGATCCATCGGAGTCAATTACGGCACCATCGCTGACAATCTCCCTCCGCCGTCCAAGGTTGCAGAGTTCCTCTTGGATTCCACCATAATCGACCGCATCAGACTCTTCGATGCCAATTCCGACATCTTACGAGCTTTCGCTCACACCGGCATCTCCGTAACCGTCACCGTCCCCAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCCGAAGAGTGGATCAAATTCAACGTCCAACCCTACGTCCCTGCCACCAACATAATCCGCATTCTAGTCGGCAACGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCGAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCGCCGGAGCCTCCTTGGAGCGGCGGATTCAAGTCTCGACACCGCACTCTCTCGGCATCTTGTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGTTACGACACGCACGTAATGAAGCCGATGCTGAGCTTCCTGAGAGAAACAAACTCGCCGTTGATGGTGAATCCGTATCCATTCTTCGGCTCCTCCGCGGAAACCCTAGACTACGCGCTCTTCCGGGCGAACCCCGGCGTGTTCGATTCAGACTCGGGGGTTTTATACACCAACATGTTGGACGCTCAATTGGACGCTGTTTACTCCGCCATGAAGAGTTTGGGCTTTGAGGATCTGGACATCGTTATTGCGGAAACGGGCTGGCCCTCGAAGGGTGACCCGGCCCAGTTCGGCGTCGGCCCAAAAGAAGCCGCCGACTACAACGGGAATCTCATGCGACATGTGGTATCGGGCGTGGGGACGCCGCTGATGCCGAACCGGACGTTCGAGACGTACATTTTCGCTCTGTTCAACGAGGATCTGAAGCCGGGCCCGGTTGGTGAGAGGAACTTTGGGCTATTTGAACCGGATCTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAGTCGTCTAATCCCAGAGCCCATCACGGCCCAAAAAGTCCAAGTCTGGGCCCAAGCCCAAGCCCAAGCCCAGGCCCAGTGACTGATGGTAAAAGATGGTGCCTGCCCAAATCTGGAGCCGACTCGGAGGCCTTGCAGAGAAACATTGATTACGTTTGTGGGCTGGGCTTGGATTGTGGGCCGATTCAAGAAAATGGGCCGTGCTTTGTTCCCAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCGTACTTTCAATCCACCGAAGGGAACGATTCTGATTGCGATTTTGAGCACACGGGCGCCCTAACCACTGTGGATCCAAGTAAGTACTAA

Coding sequence (CDS)

ATGCCATTTCCAATTCACTCTTCTCACTCTCCTCCATCTTCATCACCTCCCATGGCTTCCTCTTTAGCTCCCTCCCACCTTCTTCTTCTTTACACTTCAACTCCTACTTTCCTTCTTCTTCTTCTTCTTCTTCTCCCCACTCTCATCCAAATCCAAGGAGCCCAAGGTGGATCCATCGGAGTCAATTACGGCACCATCGCTGACAATCTCCCTCCGCCGTCCAAGGTTGCAGAGTTCCTCTTGGATTCCACCATAATCGACCGCATCAGACTCTTCGATGCCAATTCCGACATCTTACGAGCTTTCGCTCACACCGGCATCTCCGTAACCGTCACCGTCCCCAACGATCAAATCCCCCGCTTAGCCAAGCCGAATTTCGCCGAAGAGTGGATCAAATTCAACGTCCAACCCTACGTCCCTGCCACCAACATAATCCGCATTCTAGTCGGCAACGAAGTCTTATCCACCGCCAACAAATTGCTCATCGCGAACCTCGTTCCAGCAATGCAGAGCCTCCACACAGCCCTCGCCGGAGCCTCCTTGGAGCGGCGGATTCAAGTCTCGACACCGCACTCTCTCGGCATCTTGTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGTTACGACACGCACGTAATGAAGCCGATGCTGAGCTTCCTGAGAGAAACAAACTCGCCGTTGATGGTGAATCCGTATCCATTCTTCGGCTCCTCCGCGGAAACCCTAGACTACGCGCTCTTCCGGGCGAACCCCGGCGTGTTCGATTCAGACTCGGGGGTTTTATACACCAACATGTTGGACGCTCAATTGGACGCTGTTTACTCCGCCATGAAGAGTTTGGGCTTTGAGGATCTGGACATCGTTATTGCGGAAACGGGCTGGCCCTCGAAGGGTGACCCGGCCCAGTTCGGCGTCGGCCCAAAAGAAGCCGCCGACTACAACGGGAATCTCATGCGACATGTGGTATCGGGCGTGGGGACGCCGCTGATGCCGAACCGGACGTTCGAGACGTACATTTTCGCTCTGTTCAACGAGGATCTGAAGCCGGGCCCGGTTGGTGAGAGGAACTTTGGGCTATTTGAACCGGATCTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAGTCGTCTAATCCCAGAGCCCATCACGGCCCAAAAAGTCCAAGTCTGGGCCCAAGCCCAAGCCCAAGCCCAGGCCCAGTGACTGATGGTAAAAGATGGTGCCTGCCCAAATCTGGAGCCGACTCGGAGGCCTTGCAGAGAAACATTGATTACGTTTGTGGGCTGGGCTTGGATTGTGGGCCGATTCAAGAAAATGGGCCGTGCTTTGTTCCCAATACGGTCCGAGCCCATGCTGCTTACGTCATGAACGCGTACTTTCAATCCACCGAAGGGAACGATTCTGATTGCGATTTTGAGCACACGGGCGCCCTAACCACTGTGGATCCAAGTAAGTACTAA

Protein sequence

MPFPIHSSHSPPSSSPPMASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPSKY

Homology

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BLAST of Spg035494.1 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-241
Identity = 421/487 (86.45%), Postives = 444/487 (91.17%), Query Frame = 0


Query: 6   HSSHSPPSSSPPMASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGT 65
           H  H  P   PPMAS   PSHLL ++ ST   +   LLLLPTLIQIQGA+ GSIGVNYGT
Sbjct: 21  HLHHITP---PPMASPFTPSHLLFVHIST---VSTFLLLLPTLIQIQGAKDGSIGVNYGT 80

Query: 66  IADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPN 125
           +ADNLPPPSKVA FLLDSTIIDR+RLFDAN DILRAFAHTGISV++T+PNDQIPRLAKPN
Sbjct: 81  LADNLPPPSKVAAFLLDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPN 140

Query: 126 FAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRI 185
           FAEEWIKFN++PY+PATNIIR+LVGNEVLSTANKLLIANLVPAMQSLHTAL  ASL+RRI
Sbjct: 141 FAEEWIKFNIEPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRI 200

Query: 186 QVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETL 245
           Q+STPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFF  +AE L
Sbjct: 201 QISTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFACTAENL 260

Query: 246 DYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQ 305
           DYALFR NPGVFDSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGD AQ
Sbjct: 261 DYALFRPNPGVFDSDSGILYTNMLDAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGDQAQ 320

Query: 306 FGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEP 365
            GVGPKEAADYNGNLMRHVVSG GTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEP
Sbjct: 321 IGVGPKEAADYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEP 380

Query: 366 DLSPVYEIGILRPTAQSSNPRAHHGP--KSPSLGPSPSPSPGPVTDGKRWCLPKSGADSE 425
           DLSPVYEIGILRPTAQSS P+AH GP   S S   SPSPSP PV++GKRWCLPK+GADSE
Sbjct: 381 DLSPVYEIGILRPTAQSSTPKAHQGPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGADSE 440

Query: 426 ALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGA 485
           ALQRNIDYVCGLGLDCGPIQENGPCF PNTVRAHAAYVMNAYFQ+TEGNDSDCDF+ TGA
Sbjct: 441 ALQRNIDYVCGLGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGA 500

Query: 486 LTTVDPS 491
           LTTVDPS
Sbjct: 501 LTTVDPS 501

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BLAST of Spg035494.1 vs. NCBI nr
Match: XP_023521611.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 835.5 bits (2157), Expect = 2.4e-238
Identity = 421/478 (88.08%), Postives = 441/478 (92.26%), Query Frame = 0


Query: 13  SSSPPMASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPP 72
           S S PMASS A S LL  YTS      LLLLLLPTL+ IQ A+GGSIGVNYGT+ADNLP 
Sbjct: 11  SLSSPMASSFALSRLLRAYTSIAA--TLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPL 70

Query: 73  PSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIK 132
           PSKVAEFLLDSTIIDRIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIK
Sbjct: 71  PSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIK 130

Query: 133 FNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHS 192
           FNVQPYVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL RRIQVSTPHS
Sbjct: 131 FNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHS 190

Query: 193 LGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRA 252
           LGILSNSSPPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFG++AETLDYALFR 
Sbjct: 191 LGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRP 250

Query: 253 NPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKE 312
           NPGV+DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKE
Sbjct: 251 NPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKE 310

Query: 313 AADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYE 372
           AADYNGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYE
Sbjct: 311 AADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYE 370

Query: 373 IGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYV 432
           IGILRPTAQSS PRAHHGP+    GPSPSPS  PV DGK WCLPK+ ADSEALQRNIDYV
Sbjct: 371 IGILRPTAQSSTPRAHHGPEPTPKGPSPSPS--PVADGKTWCLPKTEADSEALQRNIDYV 430

Query: 433 CGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           CGLGLDCGPIQENGPCF+PNTVRAHAAYVMNAYFQ+T+GNDSDCDF+ TG LTTVDPS
Sbjct: 431 CGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPS 483

[Close]

BLAST of Spg035494.1 vs. NCBI nr
Match: XP_023533272.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-237
Identity = 418/473 (88.37%), Postives = 438/473 (92.60%), Query Frame = 0


Query: 18  MASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVA 77
           MASS A S LL  YTS      LLLLLLPTL+ IQ A+GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASSFALSRLLRAYTSIAA--TLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 78  EFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQP 137
           EFLLDSTIIDRIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIKFNVQP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 138 YVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILS 197
           YVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL RRIQVSTPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILS 180

Query: 198 NSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVF 257
           NSSPPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFG++AETLDYALFR NPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 258 DSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYN 317
           DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKEAADYN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 318 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 377
           GNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 378 PTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGL 437
           PTAQSS PRAHHGP+    GPSPSPS  PV DGK WCLPK+ ADSEALQRNIDYVCGLGL
Sbjct: 361 PTAQSSTPRAHHGPEPTPKGPSPSPS--PVADGKTWCLPKTEADSEALQRNIDYVCGLGL 420

Query: 438 DCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           DCGPIQENGPCF+PNTVRAHAAYVMNAYFQ+T+GNDSDCDF+ TG LTTVDPS
Sbjct: 421 DCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPS 468

[Close]

BLAST of Spg035494.1 vs. NCBI nr
Match: KAG6605794.1 (Importin subunit beta-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 831.6 bits (2147), Expect = 3.4e-237
Identity = 417/473 (88.16%), Postives = 438/473 (92.60%), Query Frame = 0


Query: 18  MASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVA 77
           MASS A S LL  YTS      LLLLLLPTL+Q+Q A+GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASSFALSRLLRAYTSIAA--TLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 78  EFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQP 137
           EFLLDSTIIDRIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIKFNVQP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 138 YVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILS 197
           YVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL  RIQVSTPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNHRIQVSTPHSLGILS 180

Query: 198 NSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVF 257
           NSSPPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFG++AETLDYALFR NPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 258 DSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYN 317
           DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKEAADYN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 318 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 377
           GNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 378 PTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGL 437
           PTAQSS PRAHHGP+    GPSP PS  PV DGKRWCLPK+ ADSEALQRNIDYVCGLGL
Sbjct: 361 PTAQSSTPRAHHGPEPTPKGPSPRPS--PVADGKRWCLPKTEADSEALQRNIDYVCGLGL 420

Query: 438 DCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           DCGPIQENGPCF+PNTVRAHAAYVMNAYFQ+T+GNDSDCDF+ TG LTTVDPS
Sbjct: 421 DCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPS 468

[Close]

BLAST of Spg035494.1 vs. NCBI nr
Match: XP_022958170.1 (glucan endo-1,3-beta-glucosidase [Cucurbita moschata])

HSP 1 Score: 830.9 bits (2145), Expect = 5.9e-237
Identity = 416/473 (87.95%), Postives = 438/473 (92.60%), Query Frame = 0


Query: 18  MASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVA 77
           MASS A S LL  YTS      LL+LLLPTL+Q+Q A+GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASSFALSRLLRAYTSIAA--TLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 78  EFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQP 137
           EFLLDSTIIDRIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIKFNVQP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 138 YVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILS 197
           YVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL RRIQVSTPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILS 180

Query: 198 NSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVF 257
           NSSPPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFG++AETLDYALFR NPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 258 DSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYN 317
           DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKEAADYN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 318 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 377
           GNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 378 PTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGL 437
           PTAQSS PRAHHGP+    GPSPS    PV DGKRWCLPK+ ADSEALQRNIDYVCGLGL
Sbjct: 361 PTAQSSTPRAHHGPEPTPKGPSPS----PVADGKRWCLPKTEADSEALQRNIDYVCGLGL 420

Query: 438 DCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           DCGPIQENGPCF+PNTVRAHAAYVMNAYFQ+T+GNDSDCDF+ TG LTTVDPS
Sbjct: 421 DCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPS 466

[Close]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.3e-126
Identity = 232/461 (50.33%), Postives = 310/461 (67.25%), Query Frame = 0


Query: 40  LLLLLLPTLIQIQGAQGGS----IGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDAN 99
           LL+LL+       GA+  +    IGVNYG  ADNLP P+ VA FL   T IDR++LFDAN
Sbjct: 3   LLILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDAN 62

Query: 100 SDILRAFAHTGISVTVTVPNDQIPRLAKP----NFAEEWIKFNVQPYVPATNIIRILVGN 159
              + AFA T IS+ V++PN  +P LA      + A  WI+ N+ PYVPATN+  +L GN
Sbjct: 63  PTFISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGN 122

Query: 160 EVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSP-PSTGRFRQG 219
           E+L + +  LI +L+PAM+ L  AL    L   ++V+TPH LGIL+ S   PS   FR G
Sbjct: 123 EILLSTDTNLILSLLPAMRRLAQALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAG 182

Query: 220 YDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVFDSDSGVLYTNMLD 279
           Y+T +   ML F R+T SP MVNPYP+F    ETL+YALFR N G++D  + + YT+MLD
Sbjct: 183 YNTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLD 242

Query: 280 AQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGT 339
           AQ+DA+Y+AMK LG+ D+DI + E GWP++ +P Q GVG +EA D+N  ++R   SG GT
Sbjct: 243 AQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGT 302

Query: 340 PLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHG 399
           PLMPNRTFETY+F+LF+E+ KPGP+ ER+FGLF PD +PVY++G+LR  A  +       
Sbjct: 303 PLMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVA------- 362

Query: 400 PKSPSLGPSPSPSPGPV-TDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCF 459
             +PS  PSP+PSP P  + G +WC+ K GA+   LQ NI+Y CG  +DC PIQ  G CF
Sbjct: 363 -PTPSPNPSPNPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACF 422

Query: 460 VPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
            PN+++AHA+YVMNAY+Q+    D  CDF+ TG +T+ DPS
Sbjct: 423 SPNSLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPS 453

[Close]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 412.5 bits (1059), Expect = 6.5e-114
Identity = 209/437 (47.83%), Postives = 283/437 (64.76%), Query Frame = 0


Query: 59  IGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQI 118
           IGVNYG +ADNLPPPS+  + LL ST I ++RL+ A+  I++A A TG+ + +   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 119 PRLAK-PNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALA 178
           P LA  PN A +WI  NV P+ PA+ I+ I VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 179 GASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPF 238
             SL  +I+VST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 239 FGSSA----ETLDYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 298
           F   +    ETL + LF  N G  DS +G+ YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 299 ETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 358
           ETGW S+GD  + G     A  YNGNL+ H+ S VGTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 359 PVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRW 418
           P  ER FGLF+ DLS VY++G+ + ++ S  P                   G VT    W
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP------------------SGKVT-SSGW 385

Query: 419 CLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGND 478
           C+PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +    
Sbjct: 386 CVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQP 441

Query: 479 SDCDFEHTGALTTVDPS 491
           +DCDF  T  +T+ +PS
Sbjct: 446 TDCDFSKTATVTSQNPS 441

[Close]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.9e-109
Identity = 206/462 (44.59%), Postives = 287/462 (62.12%), Query Frame = 0


Query: 34  TPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFD 93
           T + L L+ LLL             +GVNYG ++DNLP        LL ST I ++RLF 
Sbjct: 7   TSSLLFLVFLLLQNFYSANSQ--SFLGVNYGQLSDNLPSLQATVN-LLKSTTIQKVRLFG 66

Query: 94  ANSDILRAFAHTGISVTVTVPNDQIPRLA-KPNFAEEWIKFNVQPYVPATNIIRILVGNE 153
           A   +++AFA+TG+ + +   N  IP LA  PN A +++K NV  + PA+NII I VGNE
Sbjct: 67  AEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNE 126

Query: 154 VLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYD 213
           VL++ ++ LI+ L+PAMQ++  AL  ASL  +++VST H++ +LS S PPS+G F  G  
Sbjct: 127 VLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLG 186

Query: 214 THVMKPMLSFLRETNSPLMVNPYPFFG----SSAETLDYALFRANPGVFDSDSGVLYTNM 273
              MK +L F    ++P M++PYP+F      + +TL + LF+ N G  DS +G  YTNM
Sbjct: 187 -DTMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNM 246

Query: 274 LDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGV 333
            DAQ+DAV+SA+ ++GF+D++IV+AETGWP  GD  + G     A  Y GNL+ H+ S V
Sbjct: 247 FDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKV 306

Query: 334 GTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAH 393
           GTPLMP ++ +TY+F+L++ED K G   E+ FGLF+PD S  Y++G+L+ T   + P   
Sbjct: 307 GTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTP--- 366

Query: 394 HGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPC 453
                       +P+P P   G  WC+PK G   + L  NI+Y CG G+DCGPIQ  G C
Sbjct: 367 -----------ATPTPTPKAAGS-WCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGAC 426

Query: 454 FVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           F PNTV+AHAAYVMN Y+QS   N  +CDF  T  LT  +PS
Sbjct: 427 FEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPS 449

[Close]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 3.9e-90
Identity = 179/472 (37.92%), Postives = 274/472 (58.05%), Query Frame = 0


Query: 39  LLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDI 98
           L+  + +  L  +       IG+ YG  ADNLP P++V+E L+    I  +R++DAN D+
Sbjct: 7   LVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSE-LIQHLNIKFVRIYDANIDV 66

Query: 99  LRAFAHTGISVTVTVPNDQIPRLAK-PNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTA 158
           L+AFA+TGI + + VPN  +   A+  +  + W+  N+ PY P+T I  I VG EV    
Sbjct: 67  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 126

Query: 159 NKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMK 218
           +      ++PAM+++HTAL  + L+++I++S+ HSL ILS S PPS+  F + +    +K
Sbjct: 127 DN-ATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLK 186

Query: 219 PMLSFLRETNSPLMVNPYPFFGSSAET----LDYALFRANPGVFDSDSGVLYTNMLDAQL 278
           PML FL E  SP M++ YP++     T    L+YALF ++  V D  +G+LY+NM DAQL
Sbjct: 187 PMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQL 246

Query: 279 DAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLM 338
           DA+Y A+ ++ F+ + +++ E+GWPSKG P +    P+ A  YN NL+RHV+   GTP  
Sbjct: 247 DAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAK 306

Query: 339 PNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKS 398
           P    + Y+F+LFNE+ KPG   ERN+G+F  + + VY +     T +++ P +   P +
Sbjct: 307 PGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDF---TGENTTPVS---PTN 366

Query: 399 PSLGPSPSPSPGPVTDG--------------KRWCLPKSGADSEALQRNIDYVCGLG-LD 458
            + G SPSPS  P+ +G              K+WC+  S A    LQ  +D+ CG G +D
Sbjct: 367 STTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVD 426

Query: 459 CGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           C  +Q + PCF P+TV +HA+Y  N Y+Q +  +  DC F         DPS
Sbjct: 427 CSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

[Close]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 323.9 bits (829), Expect = 3.1e-87
Identity = 183/461 (39.70%), Postives = 266/461 (57.70%), Query Frame = 0


Query: 40  LLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDIL 99
           LLLL L        +Q   IGVN GT   N+P P++V   LL S  I+R+RL+DA+  +L
Sbjct: 4   LLLLFLFLFASSALSQDSLIGVNIGTEVTNMPSPTQVVA-LLKSQNINRVRLYDADRSML 63

Query: 100 RAFAHTGISVTVTVPNDQIPRLAKPN-FAEEWIKFNVQPYVPATNIIRILVGNEVLSTAN 159
            AFAHTG+ V ++VPNDQ+  +++ N  A  W+  NV  Y PATNI  I VG+EVL++  
Sbjct: 64  LAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLT 123

Query: 160 KLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKP 219
               + LV A++ +  AL  A+L+R+I+VSTPHS  I+ +S PPS   F + +D  V+ P
Sbjct: 124 N-AASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDP-VIVP 183

Query: 220 MLSFLRETNSPLMVNPYPFF----GSSAETLDYALF---RANPGVFDSDSGVLYTNMLDA 279
           +L FL+ T SPL++N YP+F     +    LDYALF   +AN    D+++ + YTN+ DA
Sbjct: 184 LLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDA 243

Query: 280 QLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTP 339
            +DA Y AM  L F ++ IV+ E+GWPSKG P++     + A  YN NL++HV++  GTP
Sbjct: 244 IVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTP 303

Query: 340 LMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGP 399
             P     TYI+ L+NED +PGPV E+N+GLF  + +PVY + +    A  +N       
Sbjct: 304 KHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILAND------ 363

Query: 400 KSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLG-LDCGPIQENGPCFV 459
                           T  + +C+ K   D + LQ  +D+ CG G +DC  + +   C+ 
Sbjct: 364 ----------------TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYE 423

Query: 460 PNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPSK 492
           P+ V AH+ Y  NAY+Q        CDF+    +TT DPS+
Sbjct: 424 PDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSR 439

[Close]

BLAST of Spg035494.1 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.8e-237
Identity = 416/473 (87.95%), Postives = 438/473 (92.60%), Query Frame = 0


Query: 18  MASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVA 77
           MASS A S LL  YTS      LL+LLLPTL+Q+Q A+GGSIGVNYGT+ADNLP PSKVA
Sbjct: 1   MASSFALSRLLRAYTSIAA--TLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVA 60

Query: 78  EFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQP 137
           EFLLDSTIIDRIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIKFNVQP
Sbjct: 61  EFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQP 120

Query: 138 YVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILS 197
           YVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL RRIQVSTPHSLGILS
Sbjct: 121 YVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILS 180

Query: 198 NSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVF 257
           NSSPPST RFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFFG++AETLDYALFR NPGV+
Sbjct: 181 NSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVY 240

Query: 258 DSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYN 317
           DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKEAADYN
Sbjct: 241 DSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYN 300

Query: 318 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILR 377
           GNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDLSPVYEIGILR
Sbjct: 301 GNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILR 360

Query: 378 PTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGL 437
           PTAQSS PRAHHGP+    GPSPS    PV DGKRWCLPK+ ADSEALQRNIDYVCGLGL
Sbjct: 361 PTAQSSTPRAHHGPEPTPKGPSPS----PVADGKRWCLPKTEADSEALQRNIDYVCGLGL 420

Query: 438 DCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           DCGPIQENGPCF+PNTVRAHAAYVMNAYFQ+T+GNDSDCDF+ TG LTTVDPS
Sbjct: 421 DCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPS 466

[Close]

BLAST of Spg035494.1 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.8e-235
Identity = 414/478 (86.61%), Postives = 437/478 (91.42%), Query Frame = 0


Query: 13  SSSPPMASSLAPSHLLLLYTSTPTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPP 72
           S S PMASS A S LL  YTS    + LLLLLLPTL+Q+Q A+GGSIGVNYGT+ADNLP 
Sbjct: 34  SLSSPMASSFALSRLLRAYTSIA--VTLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPL 93

Query: 73  PSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIK 132
           PSKVAEFLLDSTII RIRLFDAN DILRAFAHT ISVT+TVPNDQIPRL KPNFAEEWIK
Sbjct: 94  PSKVAEFLLDSTIIGRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIK 153

Query: 133 FNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHS 192
           FNVQPYVPAT+IIRILVGNEVLSTANK+LIANLVPAMQSLHTAL+ ASL  RIQVSTPHS
Sbjct: 154 FNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNSRIQVSTPHS 213

Query: 193 LGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRA 252
           LGILSNSSPPST RFRQGYDTHV+KPMLSFL+ETNSPLMVNPYPFFG++AETLDYALFR 
Sbjct: 214 LGILSNSSPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVNPYPFFGTTAETLDYALFRP 273

Query: 253 NPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKE 312
           NPGV+DSDSG+LYTNMLDAQLDAVYSAMKSLGF DLDIVIAETGWPSKGDPAQ GVGPKE
Sbjct: 274 NPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKE 333

Query: 313 AADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYE 372
           AADYNGNL+RHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDL+PVYE
Sbjct: 334 AADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLNPVYE 393

Query: 373 IGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYV 432
           IGILRPTAQSS PRAHHGP+    GPSPS    PV DGKRWCLPK+ ADSEALQRNIDYV
Sbjct: 394 IGILRPTAQSSTPRAHHGPEPTPKGPSPS----PVADGKRWCLPKTEADSEALQRNIDYV 453

Query: 433 CGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           CGLG DCGPIQ NGPCF+PNTVRAHAAYVMNAYFQ+ +GNDSDCDF+ TG LTTVDPS
Sbjct: 454 CGLGFDCGPIQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCDFQQTGDLTTVDPS 504

[Close]

BLAST of Spg035494.1 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 399/487 (81.93%), Postives = 431/487 (88.50%), Query Frame = 0


Query: 5   IHSSHSPPSSSPPMASSLAPSHLLLLYTST-PTFLLLLLLLLPTLIQIQGAQGGSIGVNY 64
           IH  H  P +SPP   S    HLLL Y ST PTF   LLL+LPTLIQIQG  GGSIGVNY
Sbjct: 12  IHLHHLHPMASPPFTPS---HHLLLPYFSTLPTF---LLLILPTLIQIQGVNGGSIGVNY 71

Query: 65  GTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAK 124
           GT+ADNLPPPSKVA FLLD+TIIDR+RLFDA+ DILRAFAHTGISV++T+PNDQIPRL K
Sbjct: 72  GTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVK 131

Query: 125 PNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLER 184
           PNFAEEWIKFN+QPY+PATNIIR+LVGNEVLSTANKLLIANLVPAMQSLHTAL  ASL+R
Sbjct: 132 PNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDR 191

Query: 185 RIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAE 244
           RIQ+STPHSLGILSNS+PPSTGRFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFF  +AE
Sbjct: 192 RIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAE 251

Query: 245 TLDYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDP 304
            LDYALFR NPGVFDSD G+LYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP
Sbjct: 252 NLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDP 311

Query: 305 AQFGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLF 364
           +Q GVGPKEAA YNGNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLF
Sbjct: 312 SQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLF 371

Query: 365 EPDLSPVYEIGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSE 424
           EPDL+PVYEIGILRPTAQS+ PRAH GP    +    SPS   V++ KRWC+PK+ A  E
Sbjct: 372 EPDLTPVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVE 431

Query: 425 ALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGA 484
           ALQRNIDYVCGLGLDCGPIQENGPC+ PNTVR HAAYVMNAYFQ+TEG + DCDF+ TGA
Sbjct: 432 ALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGA 491

Query: 485 LTTVDPS 491
           LTTVDPS
Sbjct: 492 LTTVDPS 492

[Close]

BLAST of Spg035494.1 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.7e-227
Identity = 393/477 (82.39%), Postives = 426/477 (89.31%), Query Frame = 0


Query: 14  SSPPMASSLAPSHLLLLYTST-PTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPP 73
           +SPP   S    HLLL Y ST PTF   LLL+LPTLIQIQG  GGSIGVNYGT+ADNLPP
Sbjct: 2   ASPPFTPS---HHLLLPYFSTLPTF---LLLILPTLIQIQGVNGGSIGVNYGTVADNLPP 61

Query: 74  PSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIK 133
           PSKVA FLLD+TIIDR+RLFDA+ DILRAFAHTGISV++T+PNDQIPRL KPNFAEEWIK
Sbjct: 62  PSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIK 121

Query: 134 FNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHS 193
           FN+QPY+PATNIIR+LVGNEVLSTANKLLIANLVPAMQSLHTAL  ASL+RRIQ+STPHS
Sbjct: 122 FNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHS 181

Query: 194 LGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRA 253
           LGILSNS+PPSTGRFRQGYDTHV+KPMLSFLRETNSPLMVNPYPFF  +AE LDYALFR 
Sbjct: 182 LGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRP 241

Query: 254 NPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKE 313
           NPGVFDSD G+LYTNMLDAQLDAVYSAMKS+ FEDLDIVIAETGWPSKGDP+Q GVGPKE
Sbjct: 242 NPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKE 301

Query: 314 AADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYE 373
           AA YNGNLMRHVV+G GTPLMPNRTFETYIFALFNE+LKPGPVGERNFGLFEPDL+PVYE
Sbjct: 302 AAYYNGNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYE 361

Query: 374 IGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYV 433
           IGILRPTAQS+ PRAH GP    +    SPS   V++ KRWC+PK+ A  EALQRNIDYV
Sbjct: 362 IGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYV 421

Query: 434 CGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDP 490
           CGLGLDCGPIQENGPC+ PNTVR HAAYVMNAYFQ+TEG + DCDF+ TGALTTVDP
Sbjct: 422 CGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

[Close]

BLAST of Spg035494.1 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.9e-226
Identity = 402/496 (81.05%), Postives = 429/496 (86.49%), Query Frame = 0


Query: 1   MPFPIHSSHSPP---SSSPPMASS--LAPSHLLLLYTST-PTFLLLLLLLLPTLIQIQGA 60
           MP P   S S     +S  PMASS      HLLL Y ST PTF   LLL+LPTL QIQG 
Sbjct: 1   MPHPNSLSSSKSIFITSIHPMASSPFTLSHHLLLPYFSTLPTF---LLLILPTLFQIQGV 60

Query: 61  QGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVP 120
            GGSIGVNYGT+ADNLPPPSKVA FLLD+TIIDR+RLFDA+ DILRAFAHTGISV++T+P
Sbjct: 61  NGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIP 120

Query: 121 NDQIPRLAKPNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHT 180
           NDQIPRL KPNFAEEWIKFN+QPY+PATNIIR+LVGNEVLSTANKLLIANLVPAMQSLHT
Sbjct: 121 NDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHT 180

Query: 181 ALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNP 240
           AL  ASL+RRIQ+STPHSLGILSNS+PPST RFRQGYDTHV+KPMLSFLRETNSPLMVNP
Sbjct: 181 ALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNP 240

Query: 241 YPFFGSSAETLDYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIAE 300
           YPFF  +A+ LDYALFR NPGVFDSD G+LYTNMLDAQLDAVYSAMKSLGFEDLDIVIAE
Sbjct: 241 YPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVIAE 300

Query: 301 TGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGP 360
           TGWPSKGDP Q GVGPKEAA YNGNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGP
Sbjct: 301 TGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGP 360

Query: 361 VGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRWC 420
           +GERNFGLFEPDLSPVYEIGILRPTAQS+ PRAH G           PSP  V++ KRWC
Sbjct: 361 IGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVE-------GPSPSTVSESKRWC 420

Query: 421 LPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGNDS 480
           LPKS A  E LQRNIDYVCGLGLDCGPI+ENG CF PNTVRAHAAYVMNAYFQ+TEGND 
Sbjct: 421 LPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDF 480

Query: 481 DCDFEHTGALTTVDPS 491
           DCDF+ TG LTTVDPS
Sbjct: 481 DCDFDQTGTLTTVDPS 486

[Close]

BLAST of Spg035494.1 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 590.5 bits (1521), Expect = 1.2e-168
Identity = 287/456 (62.94%), Postives = 357/456 (78.29%), Query Frame = 0


Query: 35  PTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDA 94
           P F L +  ++  L+ +      SIGVNYGT+A+NLPPP +VAEFLL ST+I+RIRLFD 
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 95  NSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQPYVPATNIIRILVGNEVL 154
           +  IL+AFA+TGI+VTVTVPNDQIP L   + A++WI  ++QP+ P+TNIIRILVGNEV+
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 155 STANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTH 214
           STA+ LLI  L+PAMQSLHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 215 VMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVFDSDSGVLYTNMLDAQLD 274
           V+KP+LSFLR T+SP +VNPYPFFG S ETLD+ALFR NPG+FD  + +LYTNMLDAQLD
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 275 AVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMP 334
           +VYSAM  LGF D++IVI E GWPS+GD  Q GV    AA++N NL+  V SG GTPLMP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 335 NRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSP 394
           NRTFETYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS+P   + P+SP
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP--VYNPRSP 365

Query: 395 SLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTV 454
             G S           KRWC+ K+GA++ ALQRNIDYVCGLGLDC PI E G C++PNTV
Sbjct: 366 VRGSS----------SKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTV 425

Query: 455 RAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           +AH+ Y MN Y+Q+ E ++ DCDF++TG +TT+DPS
Sbjct: 426 KAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

[Close]

BLAST of Spg035494.1 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 590.5 bits (1521), Expect = 1.2e-168
Identity = 287/456 (62.94%), Postives = 357/456 (78.29%), Query Frame = 0


Query: 35  PTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDA 94
           P F L +  ++  L+ +      SIGVNYGT+A+NLPPP +VAEFLL ST+I+RIRLFD 
Sbjct: 6   PFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDT 65

Query: 95  NSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQPYVPATNIIRILVGNEVL 154
           +  IL+AFA+TGI+VTVTVPNDQIP L   + A++WI  ++QP+ P+TNIIRILVGNEV+
Sbjct: 66  DPQILQAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVI 125

Query: 155 STANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTH 214
           STA+ LLI  L+PAMQSLHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  
Sbjct: 126 STADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQ 185

Query: 215 VMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRANPGVFDSDSGVLYTNMLDAQLD 274
           V+KP+LSFLR T+SP +VNPYPFFG S ETLD+ALFR NPG+FD  + +LYTNMLDAQLD
Sbjct: 186 VLKPLLSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLD 245

Query: 275 AVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMP 334
           +VYSAM  LGF D++IVI E GWPS+GD  Q GV    AA++N NL+  V SG GTPLMP
Sbjct: 246 SVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMP 305

Query: 335 NRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSP 394
           NRTFETYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS+P   + P+SP
Sbjct: 306 NRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDP--VYNPRSP 365

Query: 395 SLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTV 454
             G S           KRWC+ K+GA++ ALQRNIDYVCGLGLDC PI E G C++PNTV
Sbjct: 366 VRGSS----------SKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTV 425

Query: 455 RAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           +AH+ Y MN Y+Q+ E ++ DCDF++TG +TT+DPS
Sbjct: 426 KAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

[Close]

BLAST of Spg035494.1 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.3e-117
Identity = 220/459 (47.93%), Postives = 298/459 (64.92%), Query Frame = 0


Query: 38  LLLLLLLLPTLIQIQGAQGGS-IGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANS 97
           L +L LLL  L  I  +   S IGVNYG ++DNLPPPS+ A+ LL ST I ++RL++A+S
Sbjct: 3   LSILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAK-LLQSTSIQKVRLYNADS 62

Query: 98  DILRAFAHTGISVTVTVPNDQIPRLAKP-NFAEEWIKFNVQPYVPATNIIRILVGNEVLS 157
            I+ +   TGI + + V N  +P +A   N A +WI  NV P+ PA+NII I VGNEVL 
Sbjct: 63  SIITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLL 122

Query: 158 TANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHV 217
           + +  L+  L+PAMQ++  AL   SL  +I+VST H++ +L NS PPS G F   Y    
Sbjct: 123 SNDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG- 182

Query: 218 MKPMLSFLRETNSPLMVNPYPFFGSSA----ETLDYALFRANPGVFDSDSGVLYTNMLDA 277
           +K +L FL +T SP  +NPYPFF   +    ETL + LF+ NPG  DS++G+ Y NM DA
Sbjct: 183 LKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDA 242

Query: 278 QLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTP 337
           Q+DAV+SA+KS+GFE +++++AETGWPS GD  + G   + A  YNGNL+ H+ S VGTP
Sbjct: 243 QVDAVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTP 302

Query: 338 LMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGP 397
           LMP ++ +TYIFALF+E+LKPGP  E++FGLF+PDLS  Y+IG+ + T+  +        
Sbjct: 303 LMPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQT-------- 362

Query: 398 KSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVP 457
                  S SP  G VT    WC+PK  A  E LQ ++D+VCG G+DCGPI   G CF P
Sbjct: 363 -------SQSPQLGKVT-SMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEP 422

Query: 458 NTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           N V +H AY MN YFQ +  N +DCDF  T  +T+ +PS
Sbjct: 423 NNVASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPS 443

[Close]

BLAST of Spg035494.1 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.7e-115
Identity = 209/437 (47.83%), Postives = 283/437 (64.76%), Query Frame = 0


Query: 59  IGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDANSDILRAFAHTGISVTVTVPNDQI 118
           IGVNYG +ADNLPPPS+  + LL ST I ++RL+ A+  I++A A TG+ + +   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVK-LLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 119 PRLAK-PNFAEEWIKFNVQPYVPATNIIRILVGNEVLSTANKLLIANLVPAMQSLHTALA 178
           P LA  PN A +WI  NV P+ PA+ I+ I VGNE+L + +  L+  L+PAMQ++  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 179 GASLERRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPF 238
             SL  +I+VST +S+ +L +S PPS+G F  GY T  +K +L FL +T SP  +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 239 FGSSA----ETLDYALFRANPGVFDSDSGVLYTNMLDAQLDAVYSAMKSLGFEDLDIVIA 298
           F   +    ETL + LF  N G  DS +G+ YTNM DAQ+DAV+SA+KS+GFE ++IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 299 ETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 358
           ETGW S+GD  + G     A  YNGNL+ H+ S VGTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 359 PVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGPKSPSLGPSPSPSPGPVTDGKRW 418
           P  ER FGLF+ DLS VY++G+ + ++ S  P                   G VT    W
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSSQTP------------------SGKVT-SSGW 385

Query: 419 CLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVPNTVRAHAAYVMNAYFQSTEGND 478
           C+PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +    
Sbjct: 386 CVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQP 441

Query: 479 SDCDFEHTGALTTVDPS 491
           +DCDF  T  +T+ +PS
Sbjct: 446 TDCDFSKTATVTSQNPS 441

[Close]

BLAST of Spg035494.1 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 406.4 bits (1043), Expect = 3.3e-113
Identity = 219/459 (47.71%), Postives = 294/459 (64.05%), Query Frame = 0


Query: 35  PTFLLLLLLLLPTLIQIQGAQGGSIGVNYGTIADNLPPPSKVAEFLLDSTIIDRIRLFDA 94
           P  + LLLLL   +     A   +IGVNYGT+  NLPPP++VA F+   T ID +++FD 
Sbjct: 5   PPSISLLLLLCAAVFLTIPAVISAIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDV 64

Query: 95  NSDILRAFAHTGISVTVTVPNDQIPRLAKPNFAEEWIKFNVQPYVPATNIIRILVGNEVL 154
           N DILRAFA TGISV VTVPN  IP LA    A  W+  N+ P+ P T I  I VGNE+L
Sbjct: 65  NPDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEIL 124

Query: 155 STANKLLIANLVPAMQSLHTALAGASLERRIQVSTPHSLGILSN--SSPPSTGRFRQGYD 214
            T +  +I NL+PAM++L+ AL  A + R ++V+T HSL I++   +  PS+GRFR G+D
Sbjct: 125 LTGDNNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWD 184

Query: 215 THVMKPMLSFLRETNSPLMVNPYPFFGSSAETLDYALFRA-NPGVFDSDSGVLYTNMLDA 274
             ++ P+L++ R T SP MVNPYP+FG   + +++A+FR     V D  +  +YTNM DA
Sbjct: 185 KGILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDA 244

Query: 275 QLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPAQFGVGPKEAADYNGNLMRHVVSGVGTP 334
            +D+ YSAMK+LG+ D++IV+ ETGWPS  D       P  AA +N N+++    G GTP
Sbjct: 245 LMDSTYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTP 304

Query: 335 LMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSNPRAHHGP 394
           LMPNR FETYIF LFNE+ KPGP  ERN+GLF  D SPVY++G+LR            G 
Sbjct: 305 LMPNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR--------NGQGGG 364

Query: 395 KSPSLGPSPSPSPGPVTDGKRWCLPKSGADSEALQRNIDYVCGLGLDCGPIQENGPCFVP 454
             P+L       P P T G +WC+ +SGA +  LQ +I++VCG G+DC PIQ  G CF P
Sbjct: 365 GRPAL-------PAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNP 424

Query: 455 NTVRAHAAYVMNAYFQSTEGNDSDCDFEHTGALTTVDPS 491
           +++R HA++VMNAYFQS    D  C+F  TG +   +PS
Sbjct: 425 SSLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPS 443

The following BLAST results are available for this feature:

BLAST of Spg035494.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906871.1	1.8e-241	86.45	glucan endo-1,3-beta-glucosidase [Benincasa hispida]	[more]
XP_023521611.1	2.4e-238	88.08	glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023533272.1	2.0e-237	88.37	glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605794.1	3.4e-237	88.16	Importin subunit beta-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022958170.1	5.9e-237	87.95	glucan endo-1,3-beta-glucosidase [Cucurbita moschata]	[more]

BLAST of Spg035494.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	3.3e-126	50.33	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q9M069	6.5e-114	47.83	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q94G86	4.9e-109	44.59	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q8VYE5	3.9e-90	37.92	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9ZU91	3.1e-87	39.70	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]

BLAST of Spg035494.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H2P3	2.8e-237	87.95	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 P...	[more]
A0A6J1K780	7.8e-235	86.61	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=...	[more]
A0A1S3AV36	1.3e-229	81.93	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV...	[more]
A0A5A7TGS6	2.7e-227	82.39	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0KJS0	3.9e-226	81.05	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3...	[more]

BLAST of Spg035494.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24318.1	1.2e-168	62.94	O-Glycosyl hydrolases family 17 protein	[more]
AT5G24318.2	1.2e-168	62.94	O-Glycosyl hydrolases family 17 protein	[more]
AT2G16230.1	1.3e-117	47.93	O-Glycosyl hydrolases family 17 protein	[more]
AT4G34480.1	4.7e-115	47.83	O-Glycosyl hydrolases family 17 protein	[more]
AT3G55430.1	3.3e-113	47.71	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 412..492 e-value: 1.7E-35 score: 133.9
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 413..482 e-value: 2.4E-19 score: 69.8
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 59..373 e-value: 2.0E-80 score: 270.7
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 288..301
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 57..380 e-value: 1.4E-119 score: 400.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 407..491 e-value: 6.6E-10 score: 41.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 378..414
None	No IPR available	PANTHER	PTHR32227:SF62	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN	coord: 50..489
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 50..489
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 59..374

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg035494	Spg035494	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg035494.1-cds	Spg035494.1-cds-scaffold3:2306984..2307140	CDS
Spg035494.1-cds	Spg035494.1-cds-scaffold3:2307258..2308237	CDS
Spg035494.1-cds	Spg035494.1-cds-scaffold3:2308334..2308675	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg035494.1	Spg035494.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding