Spg033938.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg033938.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold13: 34093425 .. 34096517 (+)
Sequence length1524
RNA-Seq ExpressionSpg033938.1
SyntenySpg033938.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTGAAACTTTTTAGAGTTCATGGACATTTTTTAAACAAACTCTATAGTTTAGGAGTATTTTTTATAATTTAGTCGAATAATAATAATAATAATAAAATAAGGGTAGTTTTTCTTTTTTTTTTTTTTTTTGGTTCTCTCTCTGATCATCCGCCCAGCAGATATATTGTGTGCAGAAACCAATCAGCTTCTTCCGAAGCTCACACTACCATGGCCAGTGTTATAAAGCCGAATTACGCAGATCTGAGCCGCCCACTCCTCTCCAAACCAGTAGCTGACAACGGTGATATTAGCCTAGCAGATGAACATCCACCACCAAATTCTTCCACTTATCACCATTTTCTCTCCAACCTATGGCTCGAAACTCAGCAGCTATGGCTGATTGTCGGCCCTTCCATCTTCAGCCGTGTCTCTGCCTACTCCATGAACATCATCACCCAAGCTTTCGCCGGTCGCTTGGGCGACATCGAACTCGCTTCCATTTCCATAGCCAACACCGTAATCGTCGGCTTCAATTTCGGTCTTCTGGTAGGTGTTCCAACTCCCGTAATGTCATTTTGATAAATTTGAAAGCTGATGAAACTTTTTTTCAAAGGTGGGGATGGCAAGTGCTCTAGAGACGCTGTGCGGGCAGGCCTATGGAGCGAAGATGTACCACATGTTGGGGATTTACTTGCAGCGCTCGTGGATAGTGCTGTTACTCTGTTCCTTTCTGCTGCTTCCTTTTTACGTTTATGCCACTCCGCTTCTGAAGCTGATGGGACAGGCGGACGACGTGGCGGAGCAATCCGGGGTGGTGGCGGTTTGGATGATACCACTTCATTTCAGCTTTGCGTTTCAGTTTCCATTGCAGAGGTTTTTGCAATGCCAACTCAAAACCCAAGTGATCGCTTGGGTTGCTTTAGCGGGATTGGGCGTCAATATTGTTGCTAGTTGGGTTTTGGTATATGTTTTGGAACTTGGAGTGATTGGGGCAGCCATAGCTTTGGACATTTCTTGGTGGGTTCTGGTTTTTGGCTTGTATTTCTACACTGTTGGTGGTTGGTGTCCTTTGACCTGGACTGGCTTCTCCATTCAAGCCTTCCAAGGGCTCTGGGAATTCATTAGACTCTCTTCTGCTGCTGGTCTCATGCTCTGGTAATTTCTTTCTTTGAATTGGTCGAGAGTATATATATGTCTTAACATCCAATTTGAAATAGGAAATTGGAAATGACATTTTGTGTGGTTAATTTGGGTGTGCGCAGTTTAGAAAACTGGTATTATCGGATCCTTGTACTACTGACGGGAAATCTTAAAAATGCCACGATTGCCGTAGATGCATTGTCCGTATGGTATGATAAATTATATTATTTTCTTCATAATTGGTCCTTGTACTATTTTTGTTTTCGATTAATAATTGTACGGAATTAATACTTTTTGTCGACGGGTCAAACTTTAATTTGCAGCATGAGCATCAATGGATGGGAGATGATGATTCCTTTAGGCTTCTTTGTTGGCACCGGGTACCCTTTCTTCTTTTATGGAAAAATTACATTTTTCTTCTTTTTGGAGTTTGGATAATAATACATCTAATTTATAAGATATATGGATTATTTCACTCATTGTCAATTGGTTTTGAGATAGAATCACATATTATCCTAATATTTAGAGTAAGATTTTGAAACTTAAGAAGCTAAATAGATTTATCATCCAATCTTTATGGGCGAAAAGGATCATTTTTCTTTTCTTATTACAACATGAAGAAAAAGATCAAGTTTCTGGGTAGGAATTGAGCCAGAACACTAGTTTTATACTAAAAAGTTTGTGAAGAACAGAGTAAGAGTGGCGAATGAGCTAGGAGCTGGCAATGGAAAAGGAGCCAAATTTGCTACAATTGTATCAGTGGTGCAATCGTCAGTGATTGGAGTGGTGATATGCATTGTTACAATGATCCTTCATGACAAGATTGCCTTCATTTTTACAACTAGTAGCAGCGTGGTGGAAGCTGTAGATACACTCTCAAATATTCTAGCCATCACCATTCTCTTAAACAGCATCCAACCCGTTCTCTCAGGTTCCTAAAATCAAAACCAATGCCATTTTAAATTTGGGATGTATATGAATGAAATGTTTTTGTTTGATCAGTTACAAAAGAAGTGAGAAATTAAAAATGGTGTTGTTAATGAATGAATATGCAGGAGTGGCAGTTGGATCAGGTTGGCAATCTTGGGTTGCGTATATAAATATTGGCTGCTATTATATAATTGGTCTCCCTCTTGGCTTCATTATGGAATGGGTTTTCCACTTTGGAGTATCGGTGAATCAAAAATCTTCCTTGCATGCAGTTTTGTTTATATATAATATTGATTTATCTTGATATATGTTTATATGAGATGCTAATTAATATATATGCAATAATAGGGGATTTGGCTTGGGATGATATTTGGTGGGACTGCACTTCAAACAATAATATTAATCATCATCACAGCCAGAACTGATTGGGATAAAGAGGTAAATATTAATTGATTTACATTTCCATAGGCTTACGTTACATTAGTGCATGTTCGGCATGTTCCGGAGTGATTTTAAAATGGAAAAAATCAATTTTGTCTTATTTAAAATCACTAAATTTTAATAATATGAAATTAAAATCAATTTTAAGTAGGTAAAATCAAACATTGAAATTGATTTTGAATAATTAAAGGTATGTTTTGGAGTAATTTCGAACATGAAAAAAGTGATTTTAGTCATTTTGAAATCACTCTCAAACATGCACTTAGTATAAGTGGCTACCCCTTGTCATGTTATCTAATTTATTTTACTTCAATTTCAGGCAAAGAAAGCACAAGAGCACCTAAAGAAGTGGTCTAAGCACTAATCAAGATCATATAATAGCTTCTCTTGATCCATGGTAACATCTGACCTCTAGTTATTGAGGTCTTGTATTTGAAAATTGAAATGATGTGGTTTAATTTTGTGATGAAATGGTTGGCACTCAATTGGCAACCTCCAACTTACAAAAATAACTATATTCCAACAAGTTAGGAGAGTGCACACTTTGCTTGTCTACTCTTAGCTTGGATAATATAATTGAGATGACATGTATTTCCAAC

mRNA sequence

ATGGCCAGTGTTATAAAGCCGAATTACGCAGATCTGAGCCGCCCACTCCTCTCCAAACCAGTAGCTGACAACGGTGATATTAGCCTAGCAGATGAACATCCACCACCAAATTCTTCCACTTATCACCATTTTCTCTCCAACCTATGGCTCGAAACTCAGCAGCTATGGCTGATTGTCGGCCCTTCCATCTTCAGCCGTGTCTCTGCCTACTCCATGAACATCATCACCCAAGCTTTCGCCGGTCGCTTGGGCGACATCGAACTCGCTTCCATTTCCATAGCCAACACCGTAATCGTCGGCTTCAATTTCGGTCTTCTGGTGGGGATGGCAAGTGCTCTAGAGACGCTGTGCGGGCAGGCCTATGGAGCGAAGATGTACCACATGTTGGGGATTTACTTGCAGCGCTCGTGGATAGTGCTGTTACTCTGTTCCTTTCTGCTGCTTCCTTTTTACGTTTATGCCACTCCGCTTCTGAAGCTGATGGGACAGGCGGACGACGTGGCGGAGCAATCCGGGGTGGTGGCGGTTTGGATGATACCACTTCATTTCAGCTTTGCGTTTCAGTTTCCATTGCAGAGGTTTTTGCAATGCCAACTCAAAACCCAAGTGATCGCTTGGGTTGCTTTAGCGGGATTGGGCGTCAATATTGTTGCTAGTTGGGTTTTGGTATATGTTTTGGAACTTGGAGTGATTGGGGCAGCCATAGCTTTGGACATTTCTTGGTGGGTTCTGGTTTTTGGCTTGTATTTCTACACTGTTGGTGGTTGGTGTCCTTTGACCTGGACTGGCTTCTCCATTCAAGCCTTCCAAGGGCTCTGGGAATTCATTAGACTCTCTTCTGCTGCTGGTCTCATGCTCTGTTTAGAAAACTGGTATTATCGGATCCTTGTACTACTGACGGGAAATCTTAAAAATGCCACGATTGCCGTAGATGCATTGTCCGTATGCATGAGCATCAATGGATGGGAGATGATGATTCCTTTAGGCTTCTTTGTTGGCACCGGAGTAAGAGTGGCGAATGAGCTAGGAGCTGGCAATGGAAAAGGAGCCAAATTTGCTACAATTGTATCAGTGGTGCAATCGTCAGTGATTGGAGTGGTGATATGCATTGTTACAATGATCCTTCATGACAAGATTGCCTTCATTTTTACAACTAGTAGCAGCGTGGTGGAAGCTGTAGATACACTCTCAAATATTCTAGCCATCACCATTCTCTTAAACAGCATCCAACCCGTTCTCTCAGGAGTGGCAGTTGGATCAGGTTGGCAATCTTGGGTTGCGTATATAAATATTGGCTGCTATTATATAATTGGTCTCCCTCTTGGCTTCATTATGGAATGGGTTTTCCACTTTGGAGTATCGGTGAATCAAAAATCTTCCTTGCATGCAGGGATTTGGCTTGGGATGATATTTGGTGGGACTGCACTTCAAACAATAATATTAATCATCATCACAGCCAGAACTGATTGGGATAAAGAGGCAAAGAAAGCACAAGAGCACCTAAAGAAGTGGTCTAAGCACTAA

Coding sequence (CDS)

ATGGCCAGTGTTATAAAGCCGAATTACGCAGATCTGAGCCGCCCACTCCTCTCCAAACCAGTAGCTGACAACGGTGATATTAGCCTAGCAGATGAACATCCACCACCAAATTCTTCCACTTATCACCATTTTCTCTCCAACCTATGGCTCGAAACTCAGCAGCTATGGCTGATTGTCGGCCCTTCCATCTTCAGCCGTGTCTCTGCCTACTCCATGAACATCATCACCCAAGCTTTCGCCGGTCGCTTGGGCGACATCGAACTCGCTTCCATTTCCATAGCCAACACCGTAATCGTCGGCTTCAATTTCGGTCTTCTGGTGGGGATGGCAAGTGCTCTAGAGACGCTGTGCGGGCAGGCCTATGGAGCGAAGATGTACCACATGTTGGGGATTTACTTGCAGCGCTCGTGGATAGTGCTGTTACTCTGTTCCTTTCTGCTGCTTCCTTTTTACGTTTATGCCACTCCGCTTCTGAAGCTGATGGGACAGGCGGACGACGTGGCGGAGCAATCCGGGGTGGTGGCGGTTTGGATGATACCACTTCATTTCAGCTTTGCGTTTCAGTTTCCATTGCAGAGGTTTTTGCAATGCCAACTCAAAACCCAAGTGATCGCTTGGGTTGCTTTAGCGGGATTGGGCGTCAATATTGTTGCTAGTTGGGTTTTGGTATATGTTTTGGAACTTGGAGTGATTGGGGCAGCCATAGCTTTGGACATTTCTTGGTGGGTTCTGGTTTTTGGCTTGTATTTCTACACTGTTGGTGGTTGGTGTCCTTTGACCTGGACTGGCTTCTCCATTCAAGCCTTCCAAGGGCTCTGGGAATTCATTAGACTCTCTTCTGCTGCTGGTCTCATGCTCTGTTTAGAAAACTGGTATTATCGGATCCTTGTACTACTGACGGGAAATCTTAAAAATGCCACGATTGCCGTAGATGCATTGTCCGTATGCATGAGCATCAATGGATGGGAGATGATGATTCCTTTAGGCTTCTTTGTTGGCACCGGAGTAAGAGTGGCGAATGAGCTAGGAGCTGGCAATGGAAAAGGAGCCAAATTTGCTACAATTGTATCAGTGGTGCAATCGTCAGTGATTGGAGTGGTGATATGCATTGTTACAATGATCCTTCATGACAAGATTGCCTTCATTTTTACAACTAGTAGCAGCGTGGTGGAAGCTGTAGATACACTCTCAAATATTCTAGCCATCACCATTCTCTTAAACAGCATCCAACCCGTTCTCTCAGGAGTGGCAGTTGGATCAGGTTGGCAATCTTGGGTTGCGTATATAAATATTGGCTGCTATTATATAATTGGTCTCCCTCTTGGCTTCATTATGGAATGGGTTTTCCACTTTGGAGTATCGGTGAATCAAAAATCTTCCTTGCATGCAGGGATTTGGCTTGGGATGATATTTGGTGGGACTGCACTTCAAACAATAATATTAATCATCATCACAGCCAGAACTGATTGGGATAAAGAGGCAAAGAAAGCACAAGAGCACCTAAAGAAGTGGTCTAAGCACTAA

Protein sequence

MASVIKPNYADLSRPLLSKPVADNGDISLADEHPPPNSSTYHHFLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWSKH
Homology
BLAST of Spg033938.1 vs. NCBI nr
Match: XP_022951012.1 (protein DETOXIFICATION 27-like [Cucurbita moschata])

HSP 1 Score: 767.7 bits (1981), Expect = 6.3e-218
Identity = 388/462 (83.98%), Postives = 424/462 (91.77%), Query Frame = 0

Query: 44  FLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNF 103
           F+S  W+ETQ+LWLIVGPSIFSRV+++SMNIITQAFAG LGD+ELASISIANTVIVGFNF
Sbjct: 31  FVSKFWVETQKLWLIVGPSIFSRVASFSMNIITQAFAGHLGDVELASISIANTVIVGFNF 90

Query: 104 GLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQ 163
           GLL+GMASALETLCGQA+GA+ YHMLGIYLQRSWIVL LC F LLP Y YATPLLKL+GQ
Sbjct: 91  GLLLGMASALETLCGQAFGARRYHMLGIYLQRSWIVLTLCCFFLLPVYFYATPLLKLLGQ 150

Query: 164 ADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLV 223
           ADDVAEQSGVV +W+IPLHFSFAFQFPLQRFLQCQLKTQVIAWV+LAGL VNIVASWVL+
Sbjct: 151 ADDVAEQSGVVVLWLIPLHFSFAFQFPLQRFLQCQLKTQVIAWVSLAGLVVNIVASWVLI 210

Query: 224 YVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAG 283
           YVLELGVIGAAIALDISWWVLV GLY YTVGGWC  TWTGFS+QAFQGLW+F +LS+AAG
Sbjct: 211 YVLELGVIGAAIALDISWWVLVLGLYIYTVGGWCSSTWTGFSVQAFQGLWDFTKLSAAAG 270

Query: 284 LMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELG 343
           LMLC ENWYYRILVL+TGNLKNAT+AVDALS+CMSINGWEMMIPL FF G GVRVANELG
Sbjct: 271 LMLCSENWYYRILVLMTGNLKNATVAVDALSICMSINGWEMMIPLAFFAGVGVRVANELG 330

Query: 344 AGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAIT 403
           AGNGKGAKFATIV+V QS+VIGVVIC+V MILHDKIA IFTTSSSVVEAV TLS++LAIT
Sbjct: 331 AGNGKGAKFATIVAVAQSTVIGVVICVVIMILHDKIALIFTTSSSVVEAVGTLSSLLAIT 390

Query: 404 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHA 463
           ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYY+IGLPLGFIMEWV H GV          
Sbjct: 391 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYVIGLPLGFIMEWVLHSGV---------L 450

Query: 464 GIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GIW GMIFGGTA+QTIIL+IIT RT+WD+EA+KAQEH+++WS
Sbjct: 451 GIWGGMIFGGTAVQTIILVIITVRTNWDREAEKAQEHVEEWS 483

BLAST of Spg033938.1 vs. NCBI nr
Match: XP_022131676.1 (protein DETOXIFICATION 27-like [Momordica charantia])

HSP 1 Score: 767.3 bits (1980), Expect = 8.2e-218
Identity = 396/505 (78.42%), Postives = 440/505 (87.13%), Query Frame = 0

Query: 1   MASVIKPNYADLSRPLLSKPVADNGDISLADEHPPPNSSTYHHFLSNLWLETQQLWLIVG 60
           M S+I+ +   L++PL    +  + D S A E           FLS LW+ETQQLWLIVG
Sbjct: 1   MGSIIRADAEGLNQPL----IISSKDKSRATE---SGEDRREDFLSKLWVETQQLWLIVG 60

Query: 61  PSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCGQA 120
           PSIFSRV+ YSMNIITQAFAG LGD++LASISIANTVIVGFNFGLL+GMASALETLCGQA
Sbjct: 61  PSIFSRVAGYSMNIITQAFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQA 120

Query: 121 YGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWMIP 180
           YGAK YHMLGIYLQRSWIVL LC FLLLPFY YATP+LKL+GQ DDVAEQSGVVA+W+IP
Sbjct: 121 YGAKKYHMLGIYLQRSWIVLCLCCFLLLPFYFYATPVLKLLGQPDDVAEQSGVVALWLIP 180

Query: 181 LHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALDIS 240
           LHFSFAFQFPLQRFLQ QLKTQVIAWV+L GL VNIV SWVL+YV E GVIGAAIALDIS
Sbjct: 181 LHFSFAFQFPLQRFLQSQLKTQVIAWVSLLGLLVNIVTSWVLMYVWEFGVIGAAIALDIS 240

Query: 241 WWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVLLT 300
           WWVLVFGLY YTVGGWCPLTWTGFS+QAFQGLW+F +LS+AAGLMLC ENWYYRILVL+T
Sbjct: 241 WWVLVFGLYIYTVGGWCPLTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVLMT 300

Query: 301 GNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSVVQ 360
           GNLK+AT+AVDALS+CMSINGWEMM+PL FF G GVRVANELGAGNGKGAKFATIVSV Q
Sbjct: 301 GNLKDATVAVDALSICMSINGWEMMVPLAFFAGIGVRVANELGAGNGKGAKFATIVSVAQ 360

Query: 361 SSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAVGS 420
           S+VIGVVIC+V MILHDKIA IFT+SSSVVEAVD+LSN+LAITILLNSIQPVLSGVAVGS
Sbjct: 361 STVIGVVICVVIMILHDKIALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGS 420

Query: 421 GWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQTII 480
           GWQSWVAYINIGCYY+IGLPLGF+MEWVFH GV          GIW GMIFGGTA+QTII
Sbjct: 421 GWQSWVAYINIGCYYLIGLPLGFVMEWVFHSGV---------LGIWGGMIFGGTAIQTII 480

Query: 481 LIIITARTDWDKEAKKAQEHLKKWS 506
           L+IIT RT+W++EA+ A ++++ WS
Sbjct: 481 LVIITMRTNWEQEAQNALKNVEDWS 489

BLAST of Spg033938.1 vs. NCBI nr
Match: XP_023537925.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 395/491 (80.45%), Postives = 433/491 (88.19%), Query Frame = 0

Query: 25  GDISLADEHPP--PNSSTYHH--------FLSNLWLETQQLWLIVGPSIFSRVSAYSMNI 84
           G +  AD   P  P  ST H+        F+S  W+ETQ+LWLIVGPSIFSRV+++SMNI
Sbjct: 2   GAVVRADRDGPNQPLLSTKHNITANRSEDFVSKFWVETQKLWLIVGPSIFSRVASFSMNI 61

Query: 85  ITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCGQAYGAKMYHMLGIYLQ 144
           ITQAFAG LGD+ELASISIANTVIVGFNFGLL+GMASALETLCGQA+GA+ YHMLGIYLQ
Sbjct: 62  ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAFGARRYHMLGIYLQ 121

Query: 145 RSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWMIPLHFSFAFQFPLQRF 204
           RSWIVL LC F LLP Y YATPLLKL+GQADDVAEQSG V +W+IPLHFSFAFQFPLQRF
Sbjct: 122 RSWIVLTLCCFFLLPVYFYATPLLKLLGQADDVAEQSGAVVLWLIPLHFSFAFQFPLQRF 181

Query: 205 LQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALDISWWVLVFGLYFYTVG 264
           LQCQLKTQVIAWV+LAGL VNIVASWVL+YVLELGVIGAAIALDISWWVLV GLY YTVG
Sbjct: 182 LQCQLKTQVIAWVSLAGLLVNIVASWVLIYVLELGVIGAAIALDISWWVLVLGLYIYTVG 241

Query: 265 GWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVLLTGNLKNATIAVDALS 324
           GWC  TWTGFS+QAFQGLW+F +LS+AAGLMLC ENWYYRILVL+TGNLKNAT+AVDALS
Sbjct: 242 GWCSSTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVLMTGNLKNATVAVDALS 301

Query: 325 VCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSVVQSSVIGVVICIVTMI 384
           +CMSINGWEMMIPL FF G GVRVANELGAGNGKGAKFATIV+V QS+VIGVVIC+V MI
Sbjct: 302 ICMSINGWEMMIPLAFFAGVGVRVANELGAGNGKGAKFATIVAVAQSTVIGVVICVVIMI 361

Query: 385 LHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCY 444
           LHDKIA IFTTSSSVVEAV TLS++LAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCY
Sbjct: 362 LHDKIALIFTTSSSVVEAVGTLSSLLAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCY 421

Query: 445 YIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQTIILIIITARTDWDKEA 504
           Y+IGLPLGFIMEWV H GV          GIW GMIFGGTA+QTIIL+IIT RT+WD+EA
Sbjct: 422 YVIGLPLGFIMEWVLHSGV---------LGIWGGMIFGGTAVQTIILVIITVRTNWDREA 481

Query: 505 KKAQEHLKKWS 506
           +KAQEH+++WS
Sbjct: 482 EKAQEHVEEWS 483

BLAST of Spg033938.1 vs. NCBI nr
Match: KAG7020273.1 (Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 765.4 bits (1975), Expect = 3.1e-217
Identity = 396/507 (78.11%), Postives = 438/507 (86.39%), Query Frame = 0

Query: 1   MASVIKPNYADLSRPLLSKP--VADNGDISLADEHPPPNSSTYHHFLSNLWLETQQLWLI 60
           M +V++ N    ++PLLS    +A+N                   F+S  W+ETQ+LWLI
Sbjct: 1   MGAVVRTNRDGPNQPLLSPKHNIAENRS---------------EDFVSKFWVETQKLWLI 60

Query: 61  VGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCG 120
           VGPSIFSRV+++SMNIITQAFAG LGD+ELASISIANTVIVGFNFGLL+GMASALETLCG
Sbjct: 61  VGPSIFSRVASFSMNIITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCG 120

Query: 121 QAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWM 180
           QA+GA+ YHMLGIYLQRSWIVL LC F LLP Y YATPLLKL+GQADDVAEQSGVV +W+
Sbjct: 121 QAFGARRYHMLGIYLQRSWIVLTLCCFFLLPVYFYATPLLKLLGQADDVAEQSGVVVLWL 180

Query: 181 IPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALD 240
           IPLHFSFAFQFPLQRFLQCQLKTQVIAWV+LAGL VNIVASWVL+YVLELGVIGAAIALD
Sbjct: 181 IPLHFSFAFQFPLQRFLQCQLKTQVIAWVSLAGLVVNIVASWVLIYVLELGVIGAAIALD 240

Query: 241 ISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVL 300
           ISWWVLV GLY YTVGGWC  TWTGFS+QAFQGLW+F +LS+AAGLMLC ENWYYRILVL
Sbjct: 241 ISWWVLVLGLYIYTVGGWCSSTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVL 300

Query: 301 LTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSV 360
           +TGNLKNAT+AVDALS+CMSINGWEMMIPL FF G GVRVANELGAGNGKGAKFATIV+V
Sbjct: 301 MTGNLKNATVAVDALSICMSINGWEMMIPLAFFAGVGVRVANELGAGNGKGAKFATIVAV 360

Query: 361 VQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAV 420
            QS+V GVVIC+V MILHDKIA IFTTSSSVVEAV TLS++LAITILLNSIQPVLSGVAV
Sbjct: 361 AQSTVNGVVICVVIMILHDKIALIFTTSSSVVEAVGTLSSLLAITILLNSIQPVLSGVAV 420

Query: 421 GSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQT 480
           GSGWQSWVAYINIGCYY+IGLPLGFIMEWV H GV          GIW GMIFGGTA+QT
Sbjct: 421 GSGWQSWVAYINIGCYYVIGLPLGFIMEWVLHSGV---------LGIWGGMIFGGTAVQT 480

Query: 481 IILIIITARTDWDKEAKKAQEHLKKWS 506
           IIL+IIT RT+WD+EA+KAQEH+ +WS
Sbjct: 481 IILVIITVRTNWDREAEKAQEHVDEWS 483

BLAST of Spg033938.1 vs. NCBI nr
Match: XP_023002321.1 (protein DETOXIFICATION 27-like [Cucurbita maxima])

HSP 1 Score: 764.6 bits (1973), Expect = 5.3e-217
Identity = 385/462 (83.33%), Postives = 423/462 (91.56%), Query Frame = 0

Query: 44  FLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNF 103
           F+   W+ETQ+LWLIVGPSIFSRV+++SMNIITQAFAG LGD+ELASISIANTVIVGFNF
Sbjct: 31  FIYKFWVETQKLWLIVGPSIFSRVASFSMNIITQAFAGHLGDVELASISIANTVIVGFNF 90

Query: 104 GLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQ 163
           GLL+GMASALETLCGQA+GA+ YHMLGIYLQRSWIVL LC F LLP Y YATPLLKL+GQ
Sbjct: 91  GLLLGMASALETLCGQAFGARRYHMLGIYLQRSWIVLTLCCFFLLPVYFYATPLLKLLGQ 150

Query: 164 ADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLV 223
           ADDVAEQSGVV +W+IPLHFSFAFQFPLQRFLQCQLKTQVIAWV+LAGL VNIVASWVL+
Sbjct: 151 ADDVAEQSGVVVLWLIPLHFSFAFQFPLQRFLQCQLKTQVIAWVSLAGLVVNIVASWVLI 210

Query: 224 YVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAG 283
           YVLELGVIGAAIALDISWWVLV GLY YTVGGWCPLTWTGFS+QAF GLW+F +LS+AAG
Sbjct: 211 YVLELGVIGAAIALDISWWVLVLGLYIYTVGGWCPLTWTGFSVQAFHGLWDFTKLSAAAG 270

Query: 284 LMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELG 343
           LMLC ENWYYRILVL+TGNLKNAT+AVDALS+CMSINGWEMMIPL FF G GVRVANELG
Sbjct: 271 LMLCSENWYYRILVLMTGNLKNATVAVDALSICMSINGWEMMIPLAFFAGVGVRVANELG 330

Query: 344 AGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAIT 403
           AGNGK AKFATIV+V QS+VIGVVIC+V M+LHDKIA IFTTSSSVVEAV TLS++LAIT
Sbjct: 331 AGNGKEAKFATIVAVAQSTVIGVVICVVIMVLHDKIALIFTTSSSVVEAVGTLSSLLAIT 390

Query: 404 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHA 463
           ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYY+IGLPLGFIMEWV H GV          
Sbjct: 391 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYVIGLPLGFIMEWVLHSGV---------L 450

Query: 464 GIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GIW GMIFGGTA+QTIIL+IIT RT+WD+EA+KAQEH+++W+
Sbjct: 451 GIWGGMIFGGTAVQTIILVIITVRTNWDREAEKAQEHVEEWT 483

BLAST of Spg033938.1 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.3e-170
Identity = 300/456 (65.79%), Postives = 370/456 (81.14%), Query Frame = 0

Query: 50  LETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGM 109
           +ET++LW IVGP+IFSRV+ YSM +ITQAFAG LGD+ELA+ISI N V VGFNFGLL+GM
Sbjct: 36  VETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLELAAISIVNNVTVGFNFGLLLGM 95

Query: 110 ASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAE 169
           ASALETLCGQA+GAK YHMLG+Y+QRSWIVL  C  LLLP Y++ TP+LK +GQ DD+AE
Sbjct: 96  ASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLLPTYIFTTPVLKFLGQPDDIAE 155

Query: 170 QSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELG 229
            SGVVA+W+IPLHF+F   FPLQRFLQCQLK +V A+ A   L V+I+  W+ V  L+LG
Sbjct: 156 LSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAAAVALVVHILVCWLFVDGLKLG 215

Query: 230 VIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLE 289
           V+G    + ISWWV V  L  Y+  G CPLTWTG S +A  GLWEF++LS+++G+MLCLE
Sbjct: 216 VVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEALTGLWEFLKLSASSGVMLCLE 275

Query: 290 NWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKG 349
           NWYYRIL+++TGNL+NA IAVD+LS+CM+INGWEMMIPL FF GTGVRVANELGAGNGKG
Sbjct: 276 NWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPLAFFAGTGVRVANELGAGNGKG 335

Query: 350 AKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSI 409
           A+FATIVSV QS +IG+   ++ M+LH++IA+IF++S +V++AV+ LS +LA T+LLNS+
Sbjct: 336 ARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVAVLDAVNKLSLLLAFTVLLNSV 395

Query: 410 QPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGM 469
           QPVLSGVAVGSGWQS+VAYIN+GCYY IG+PLGF+M W F  GV          GIW GM
Sbjct: 396 QPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWGFKLGV---------MGIWGGM 455

Query: 470 IFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           IFGGTA+QT+IL  IT R DW+KEA+KA   + KWS
Sbjct: 456 IFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Spg033938.1 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.8e-166
Identity = 286/457 (62.58%), Postives = 366/457 (80.09%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+ET+++W IVGPSIF+ ++ YS+ IITQAFAG LGD+ELA+ISI N   +GFN+GLL+
Sbjct: 35  IWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLLL 94

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMASALETLCGQA+GA+ Y+MLG+Y+QR WI+L LC  LLLP Y++ATP+LK +GQ+DD+
Sbjct: 95  GMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDDI 154

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
           AE +G +A+W+IP+HF+FAF FPL RFLQCQLK +VIA  A   L V+I+  W  VY  +
Sbjct: 155 AELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGYK 214

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
           LG+IG   ++++ WW+ +F L+ Y+  G C LTWTGFS +AF GL E  +LS+++G+MLC
Sbjct: 215 LGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIMLC 274

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LENWYY+IL+L+TGNL NA IAVD+LS+CMS+NGWEMMIPL FF GTGVRVANELGAGNG
Sbjct: 275 LENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGNG 334

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           KGA+FATIVS+  S +IG+   ++ +I HD+I  IF++S +V+ AVD LS +LA T+LLN
Sbjct: 335 KGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLN 394

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVAVGSGWQS+VAYIN+GCYY+IGLP G  M W+F FGV          GIW 
Sbjct: 395 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVK---------GIWA 454

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           GMIFGGTA+QT+ILIIIT R DWD EA K+   +KKW
Sbjct: 455 GMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of Spg033938.1 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-161
Identity = 283/458 (61.79%), Postives = 363/458 (79.26%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +WLET++LW IVGP+IF+RV+   + +ITQAFAG LG++ELA+ISI N VI+GFN+ L +
Sbjct: 37  IWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNNVIIGFNYSLFI 96

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMA+ALETLCGQA+GAK Y M G+YLQRSWIVL L S LLLP Y++ATP+LK MGQ DD+
Sbjct: 97  GMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATPILKFMGQPDDI 156

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
           AE SG+++VW IP HFSFAF FP+ RFLQCQLK  VIA  +   L V+I   W+ VYVLE
Sbjct: 157 AELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHIFVCWLFVYVLE 216

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
           LGVIG     ++SWW+ VF L+ YT  G CPLTWTGFS+++F  LWEF +LS+++G+M+C
Sbjct: 217 LGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFTKLSASSGIMVC 276

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LENWYYR+L+++TGNL++A I VD++S+CMSING EMM+PL FF GT VRVANELGAGNG
Sbjct: 277 LENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSVRVANELGAGNG 336

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           K A+FA I+SV QS +IG++I ++   L D+I ++F++S +V++AV+ LS +L+  ILLN
Sbjct: 337 KRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNLSILLSFAILLN 396

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVAVGSGWQS VA+IN+GCYY IGLPLG +M W+F FGV          GIW 
Sbjct: 397 SVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVK---------GIWA 456

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GMIFGGT +QT+ILI IT R DW+KEA+ A+  + KWS
Sbjct: 457 GMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Spg033938.1 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 4.4e-121
Identity = 225/458 (49.13%), Postives = 324/458 (70.74%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+E+++LW++  P+IF+R S + ++II+Q+F G LG IELA+ SI  TV++ F+ G+L+
Sbjct: 33  VWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFTVLLRFSNGILL 92

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMASALETLCGQAYGAK  HMLGIYLQRSWIVL  C+  L P Y+++ P+L  +GQ + +
Sbjct: 93  GMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGPILLALGQEERI 152

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
              + ++A+W+I ++FSF   F  Q FLQ Q K ++IA+VA   LGV++  SW+L+    
Sbjct: 153 VRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHVFLSWLLMVHFN 212

Query: 228 LGVIGAAIALDISWWV-LVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLML 287
            G+ GA  +  +++W+  +  L F T GG C  TW GFS+ AF+ LW   +LS ++G ML
Sbjct: 213 FGITGAMTSTLVAFWLPNIAQLLFVTCGG-CKDTWRGFSMMAFKDLWPVFKLSMSSGGML 272

Query: 288 CLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGN 347
           CLE WY  ILVLLTGNLKNA +A+DAL++C++ING EMMI LGF     VRV+NELG+GN
Sbjct: 273 CLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASVRVSNELGSGN 332

Query: 348 GKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILL 407
            KGAKFAT+ +V  S  +G+V+  V + L  ++++IFTTS +V   V  LS +LA +IL+
Sbjct: 333 PKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADLSPLLAFSILM 392

Query: 408 NSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIW 467
           NS+QPVLSGVAVG+GWQ +V Y+N+ CYY++G+P+G I+ +V   G+ V        G+W
Sbjct: 393 NSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYV--VGLQVK-------GVW 452

Query: 468 LGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           +GM+F G  +QT +L ++T RTDWD++   +   L +W
Sbjct: 453 IGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Spg033938.1 vs. ExPASy Swiss-Prot
Match: F4HPH2 (Protein DETOXIFICATION 20 OS=Arabidopsis thaliana OX=3702 GN=DTX20 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.6e-118
Identity = 218/457 (47.70%), Postives = 321/457 (70.24%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+E+++LW++  P+IF+R S + ++++TQAF G LG  ELA+ SI  T+++ F+ G+L+
Sbjct: 30  VWIESKKLWVVAAPAIFTRYSTFGVSMVTQAFIGHLGPTELAAYSITFTILLRFSNGILL 89

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMA AL TLCGQAYGAK Y MLGIYLQRSWIVL   +  L+P +++A P+L  +GQ + +
Sbjct: 90  GMAGALGTLCGQAYGAKQYQMLGIYLQRSWIVLTGGTICLMPVFIFAGPILLALGQEERI 149

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
              + V+A+W+I ++FSF   F  Q FLQ Q K ++I++V    LG+++  SW+LV    
Sbjct: 150 VRVARVLALWVIGINFSFVPSFTCQMFLQAQSKNKIISYVTAVSLGLHVFFSWLLVAHFN 209

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
            G+ GA  ++ I++W+ +     Y   G C  TW GFS+ AF+ LW  ++LS ++G MLC
Sbjct: 210 FGITGAMTSMLIAFWLPIIVQLLYVTCGGCKDTWRGFSMLAFKDLWPVLKLSLSSGGMLC 269

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LE WY  +LVLLTGNLKNA +A+DAL++C+SIN  EMMI LGF     VRV+NELG+GN 
Sbjct: 270 LELWYNSVLVLLTGNLKNAEVALDALAICISINALEMMIALGFLAAVSVRVSNELGSGNP 329

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           KGAKFAT+++V  S  IG+V+  V + L  +I++IFTTS +V   V  LS +LA +ILLN
Sbjct: 330 KGAKFATLIAVFTSLSIGIVLFFVFLFLRGRISYIFTTSEAVAAEVADLSPLLAFSILLN 389

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVA+G+GWQ +VAY+N+ CYY++G+P+G I+ +V   G+ V        G+W+
Sbjct: 390 SVQPVLSGVAIGAGWQGYVAYVNLACYYLVGIPIGVILGYV--VGLQVK-------GVWI 449

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           GM+F G  +QT +L ++T RTDWD++   +  ++ +W
Sbjct: 450 GMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRNINRW 476

BLAST of Spg033938.1 vs. ExPASy TrEMBL
Match: A0A6J1GGG7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453988 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 3.1e-218
Identity = 388/462 (83.98%), Postives = 424/462 (91.77%), Query Frame = 0

Query: 44  FLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNF 103
           F+S  W+ETQ+LWLIVGPSIFSRV+++SMNIITQAFAG LGD+ELASISIANTVIVGFNF
Sbjct: 31  FVSKFWVETQKLWLIVGPSIFSRVASFSMNIITQAFAGHLGDVELASISIANTVIVGFNF 90

Query: 104 GLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQ 163
           GLL+GMASALETLCGQA+GA+ YHMLGIYLQRSWIVL LC F LLP Y YATPLLKL+GQ
Sbjct: 91  GLLLGMASALETLCGQAFGARRYHMLGIYLQRSWIVLTLCCFFLLPVYFYATPLLKLLGQ 150

Query: 164 ADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLV 223
           ADDVAEQSGVV +W+IPLHFSFAFQFPLQRFLQCQLKTQVIAWV+LAGL VNIVASWVL+
Sbjct: 151 ADDVAEQSGVVVLWLIPLHFSFAFQFPLQRFLQCQLKTQVIAWVSLAGLVVNIVASWVLI 210

Query: 224 YVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAG 283
           YVLELGVIGAAIALDISWWVLV GLY YTVGGWC  TWTGFS+QAFQGLW+F +LS+AAG
Sbjct: 211 YVLELGVIGAAIALDISWWVLVLGLYIYTVGGWCSSTWTGFSVQAFQGLWDFTKLSAAAG 270

Query: 284 LMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELG 343
           LMLC ENWYYRILVL+TGNLKNAT+AVDALS+CMSINGWEMMIPL FF G GVRVANELG
Sbjct: 271 LMLCSENWYYRILVLMTGNLKNATVAVDALSICMSINGWEMMIPLAFFAGVGVRVANELG 330

Query: 344 AGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAIT 403
           AGNGKGAKFATIV+V QS+VIGVVIC+V MILHDKIA IFTTSSSVVEAV TLS++LAIT
Sbjct: 331 AGNGKGAKFATIVAVAQSTVIGVVICVVIMILHDKIALIFTTSSSVVEAVGTLSSLLAIT 390

Query: 404 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHA 463
           ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYY+IGLPLGFIMEWV H GV          
Sbjct: 391 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYVIGLPLGFIMEWVLHSGV---------L 450

Query: 464 GIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GIW GMIFGGTA+QTIIL+IIT RT+WD+EA+KAQEH+++WS
Sbjct: 451 GIWGGMIFGGTAVQTIILVIITVRTNWDREAEKAQEHVEEWS 483

BLAST of Spg033938.1 vs. ExPASy TrEMBL
Match: A0A6J1BQ65 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 4.0e-218
Identity = 396/505 (78.42%), Postives = 440/505 (87.13%), Query Frame = 0

Query: 1   MASVIKPNYADLSRPLLSKPVADNGDISLADEHPPPNSSTYHHFLSNLWLETQQLWLIVG 60
           M S+I+ +   L++PL    +  + D S A E           FLS LW+ETQQLWLIVG
Sbjct: 1   MGSIIRADAEGLNQPL----IISSKDKSRATE---SGEDRREDFLSKLWVETQQLWLIVG 60

Query: 61  PSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCGQA 120
           PSIFSRV+ YSMNIITQAFAG LGD++LASISIANTVIVGFNFGLL+GMASALETLCGQA
Sbjct: 61  PSIFSRVAGYSMNIITQAFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQA 120

Query: 121 YGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWMIP 180
           YGAK YHMLGIYLQRSWIVL LC FLLLPFY YATP+LKL+GQ DDVAEQSGVVA+W+IP
Sbjct: 121 YGAKKYHMLGIYLQRSWIVLCLCCFLLLPFYFYATPVLKLLGQPDDVAEQSGVVALWLIP 180

Query: 181 LHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALDIS 240
           LHFSFAFQFPLQRFLQ QLKTQVIAWV+L GL VNIV SWVL+YV E GVIGAAIALDIS
Sbjct: 181 LHFSFAFQFPLQRFLQSQLKTQVIAWVSLLGLLVNIVTSWVLMYVWEFGVIGAAIALDIS 240

Query: 241 WWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVLLT 300
           WWVLVFGLY YTVGGWCPLTWTGFS+QAFQGLW+F +LS+AAGLMLC ENWYYRILVL+T
Sbjct: 241 WWVLVFGLYIYTVGGWCPLTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVLMT 300

Query: 301 GNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSVVQ 360
           GNLK+AT+AVDALS+CMSINGWEMM+PL FF G GVRVANELGAGNGKGAKFATIVSV Q
Sbjct: 301 GNLKDATVAVDALSICMSINGWEMMVPLAFFAGIGVRVANELGAGNGKGAKFATIVSVAQ 360

Query: 361 SSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAVGS 420
           S+VIGVVIC+V MILHDKIA IFT+SSSVVEAVD+LSN+LAITILLNSIQPVLSGVAVGS
Sbjct: 361 STVIGVVICVVIMILHDKIALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGS 420

Query: 421 GWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQTII 480
           GWQSWVAYINIGCYY+IGLPLGF+MEWVFH GV          GIW GMIFGGTA+QTII
Sbjct: 421 GWQSWVAYINIGCYYLIGLPLGFVMEWVFHSGV---------LGIWGGMIFGGTAIQTII 480

Query: 481 LIIITARTDWDKEAKKAQEHLKKWS 506
           L+IIT RT+W++EA+ A ++++ WS
Sbjct: 481 LVIITMRTNWEQEAQNALKNVEDWS 489

BLAST of Spg033938.1 vs. ExPASy TrEMBL
Match: A0A6J1KL04 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111496195 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.6e-217
Identity = 385/462 (83.33%), Postives = 423/462 (91.56%), Query Frame = 0

Query: 44  FLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNF 103
           F+   W+ETQ+LWLIVGPSIFSRV+++SMNIITQAFAG LGD+ELASISIANTVIVGFNF
Sbjct: 31  FIYKFWVETQKLWLIVGPSIFSRVASFSMNIITQAFAGHLGDVELASISIANTVIVGFNF 90

Query: 104 GLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQ 163
           GLL+GMASALETLCGQA+GA+ YHMLGIYLQRSWIVL LC F LLP Y YATPLLKL+GQ
Sbjct: 91  GLLLGMASALETLCGQAFGARRYHMLGIYLQRSWIVLTLCCFFLLPVYFYATPLLKLLGQ 150

Query: 164 ADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLV 223
           ADDVAEQSGVV +W+IPLHFSFAFQFPLQRFLQCQLKTQVIAWV+LAGL VNIVASWVL+
Sbjct: 151 ADDVAEQSGVVVLWLIPLHFSFAFQFPLQRFLQCQLKTQVIAWVSLAGLVVNIVASWVLI 210

Query: 224 YVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAG 283
           YVLELGVIGAAIALDISWWVLV GLY YTVGGWCPLTWTGFS+QAF GLW+F +LS+AAG
Sbjct: 211 YVLELGVIGAAIALDISWWVLVLGLYIYTVGGWCPLTWTGFSVQAFHGLWDFTKLSAAAG 270

Query: 284 LMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELG 343
           LMLC ENWYYRILVL+TGNLKNAT+AVDALS+CMSINGWEMMIPL FF G GVRVANELG
Sbjct: 271 LMLCSENWYYRILVLMTGNLKNATVAVDALSICMSINGWEMMIPLAFFAGVGVRVANELG 330

Query: 344 AGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAIT 403
           AGNGK AKFATIV+V QS+VIGVVIC+V M+LHDKIA IFTTSSSVVEAV TLS++LAIT
Sbjct: 331 AGNGKEAKFATIVAVAQSTVIGVVICVVIMVLHDKIALIFTTSSSVVEAVGTLSSLLAIT 390

Query: 404 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHA 463
           ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYY+IGLPLGFIMEWV H GV          
Sbjct: 391 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYVIGLPLGFIMEWVLHSGV---------L 450

Query: 464 GIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GIW GMIFGGTA+QTIIL+IIT RT+WD+EA+KAQEH+++W+
Sbjct: 451 GIWGGMIFGGTAVQTIILVIITVRTNWDREAEKAQEHVEEWT 483

BLAST of Spg033938.1 vs. ExPASy TrEMBL
Match: A0A6J1BQ61 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.6e-214
Identity = 379/462 (82.03%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 44  FLSNLWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNF 103
           F S LW ETQQLW+IVGP+IF+RV+ ++MN+ITQ FAG LGD++LASISIANTVIVGFNF
Sbjct: 24  FWSKLWRETQQLWVIVGPTIFARVATFTMNVITQVFAGHLGDVQLASISIANTVIVGFNF 83

Query: 104 GLLVGMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQ 163
           GLL+GMASALETLCGQAYGAK YHMLGIYLQRSWIVL LC FLLLPFYVY TP+LKL+G 
Sbjct: 84  GLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFLCCFLLLPFYVYTTPVLKLLGH 143

Query: 164 ADDVAEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLV 223
            DDVAE+SGVVA+W+IPLHFSFAFQFPLQ FLQCQ KT VIAWV+L GL VN+V+SWVL+
Sbjct: 144 EDDVAEESGVVAIWLIPLHFSFAFQFPLQTFLQCQHKTMVIAWVSLVGLIVNVVSSWVLI 203

Query: 224 YVLELGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAG 283
           Y  ELGVIGAAIALD+SWWVL FGLYFYTVGGWCPLTWTGFSIQAF GLWEF++LS+AAG
Sbjct: 204 YEWELGVIGAAIALDVSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLKLSTAAG 263

Query: 284 LMLCLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELG 343
           LMLCLENWY+RILVL+TGNL+NAT AVDALSVCMSINGWEMMIP+ FF G GVRVANELG
Sbjct: 264 LMLCLENWYFRILVLMTGNLENATTAVDALSVCMSINGWEMMIPIAFFAGIGVRVANELG 323

Query: 344 AGNGKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAIT 403
           AGNGKGAKFATIVSV QS +IGVVIC+V M  HDKIA IFT+SSSVVEAVD+LSN+LAIT
Sbjct: 324 AGNGKGAKFATIVSVAQSILIGVVICVVIMTFHDKIALIFTSSSSVVEAVDSLSNLLAIT 383

Query: 404 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHA 463
           ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFH GVS         
Sbjct: 384 ILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHSGVS--------- 443

Query: 464 GIWLGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GIW GM++GGTALQTIILIIIT RTDW+KEA+KAQ H+  WS
Sbjct: 444 GIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVDNWS 476

BLAST of Spg033938.1 vs. ExPASy TrEMBL
Match: A0A6J1BQC7 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 5.0e-213
Identity = 385/496 (77.62%), Postives = 431/496 (86.90%), Query Frame = 0

Query: 10  ADLSRPLLSKPVADNGDISLADEHPPPNSSTYHHFLSNLWLETQQLWLIVGPSIFSRVSA 69
           ADL+RPLL     + GD    DE+     S Y    S +W+ETQQLW IVGP+IF+RV+ 
Sbjct: 2   ADLNRPLLISTSNNGGD----DEN---GRSKY----SKVWVETQQLWAIVGPTIFARVAN 61

Query: 70  YSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGMASALETLCGQAYGAKMYHML 129
           YSMN+ITQ FAG LGD++LASISIANTVIVGFNFGLL+GMASALETLCGQAYGAK YHML
Sbjct: 62  YSMNVITQVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHML 121

Query: 130 GIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAEQSGVVAVWMIPLHFSFAFQF 189
           GIYLQRSWIVL  C FLLLPFYVYA P+LK +GQ D+VAE+SG+VA+W+IPLHFSFAFQF
Sbjct: 122 GIYLQRSWIVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQF 181

Query: 190 PLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELGVIGAAIALDISWWVLVFGLY 249
           PLQ FLQCQ KT VIAWV+LAGL +N+V+SWVL+Y  ELGVIGAAIALD+SWWVL FGLY
Sbjct: 182 PLQTFLQCQQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLY 241

Query: 250 FYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLENWYYRILVLLTGNLKNATIA 309
           FYTVGGWCPLTWTGFSIQAF GLWEF++LS+AAGLMLCLENWY+RILVL+TGNL+NATIA
Sbjct: 242 FYTVGGWCPLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIA 301

Query: 310 VDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKGAKFATIVSVVQSSVIGVVIC 369
           VDALSVCMSINGWEMMIP+ FF G GVRVANELGAGNGK AKFATIVSV +S+ IG+VIC
Sbjct: 302 VDALSVCMSINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVIC 361

Query: 370 IVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSIQPVLSGVAVGSGWQSWVAYI 429
            + MI H KIA IFT+SS VVEAVDTLSN+LAITILLNS+QPVLSGVAVGSGWQSWVAYI
Sbjct: 362 AIIMIFHGKIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGVAVGSGWQSWVAYI 421

Query: 430 NIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGMIFGGTALQTIILIIITARTD 489
           NIGCYYIIGLPLGFIMEWVFH GVS         GIW GM++GGTALQTIILIIIT RTD
Sbjct: 422 NIGCYYIIGLPLGFIMEWVFHSGVS---------GIWAGMLYGGTALQTIILIIITIRTD 477

Query: 490 WDKEAKKAQEHLKKWS 506
           W+KEA+KAQ H+++WS
Sbjct: 482 WEKEAEKAQMHVERWS 477

BLAST of Spg033938.1 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 600.9 bits (1548), Expect = 9.5e-172
Identity = 300/456 (65.79%), Postives = 370/456 (81.14%), Query Frame = 0

Query: 50  LETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLVGM 109
           +ET++LW IVGP+IFSRV+ YSM +ITQAFAG LGD+ELA+ISI N V VGFNFGLL+GM
Sbjct: 36  VETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLELAAISIVNNVTVGFNFGLLLGM 95

Query: 110 ASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDVAE 169
           ASALETLCGQA+GAK YHMLG+Y+QRSWIVL  C  LLLP Y++ TP+LK +GQ DD+AE
Sbjct: 96  ASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLLPTYIFTTPVLKFLGQPDDIAE 155

Query: 170 QSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLELG 229
            SGVVA+W+IPLHF+F   FPLQRFLQCQLK +V A+ A   L V+I+  W+ V  L+LG
Sbjct: 156 LSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAAAVALVVHILVCWLFVDGLKLG 215

Query: 230 VIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLCLE 289
           V+G    + ISWWV V  L  Y+  G CPLTWTG S +A  GLWEF++LS+++G+MLCLE
Sbjct: 216 VVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEALTGLWEFLKLSASSGVMLCLE 275

Query: 290 NWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNGKG 349
           NWYYRIL+++TGNL+NA IAVD+LS+CM+INGWEMMIPL FF GTGVRVANELGAGNGKG
Sbjct: 276 NWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPLAFFAGTGVRVANELGAGNGKG 335

Query: 350 AKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLNSI 409
           A+FATIVSV QS +IG+   ++ M+LH++IA+IF++S +V++AV+ LS +LA T+LLNS+
Sbjct: 336 ARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVAVLDAVNKLSLLLAFTVLLNSV 395

Query: 410 QPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWLGM 469
           QPVLSGVAVGSGWQS+VAYIN+GCYY IG+PLGF+M W F  GV          GIW GM
Sbjct: 396 QPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWGFKLGV---------MGIWGGM 455

Query: 470 IFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           IFGGTA+QT+IL  IT R DW+KEA+KA   + KWS
Sbjct: 456 IFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Spg033938.1 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 585.5 bits (1508), Expect = 4.1e-167
Identity = 286/457 (62.58%), Postives = 366/457 (80.09%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+ET+++W IVGPSIF+ ++ YS+ IITQAFAG LGD+ELA+ISI N   +GFN+GLL+
Sbjct: 35  IWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLLL 94

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMASALETLCGQA+GA+ Y+MLG+Y+QR WI+L LC  LLLP Y++ATP+LK +GQ+DD+
Sbjct: 95  GMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDDI 154

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
           AE +G +A+W+IP+HF+FAF FPL RFLQCQLK +VIA  A   L V+I+  W  VY  +
Sbjct: 155 AELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGYK 214

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
           LG+IG   ++++ WW+ +F L+ Y+  G C LTWTGFS +AF GL E  +LS+++G+MLC
Sbjct: 215 LGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIMLC 274

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LENWYY+IL+L+TGNL NA IAVD+LS+CMS+NGWEMMIPL FF GTGVRVANELGAGNG
Sbjct: 275 LENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGNG 334

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           KGA+FATIVS+  S +IG+   ++ +I HD+I  IF++S +V+ AVD LS +LA T+LLN
Sbjct: 335 KGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLN 394

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVAVGSGWQS+VAYIN+GCYY+IGLP G  M W+F FGV          GIW 
Sbjct: 395 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVK---------GIWA 454

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           GMIFGGTA+QT+ILIIIT R DWD EA K+   +KKW
Sbjct: 455 GMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of Spg033938.1 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 571.2 bits (1471), Expect = 8.1e-163
Identity = 283/458 (61.79%), Postives = 363/458 (79.26%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +WLET++LW IVGP+IF+RV+   + +ITQAFAG LG++ELA+ISI N VI+GFN+ L +
Sbjct: 37  IWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNNVIIGFNYSLFI 96

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMA+ALETLCGQA+GAK Y M G+YLQRSWIVL L S LLLP Y++ATP+LK MGQ DD+
Sbjct: 97  GMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATPILKFMGQPDDI 156

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
           AE SG+++VW IP HFSFAF FP+ RFLQCQLK  VIA  +   L V+I   W+ VYVLE
Sbjct: 157 AELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHIFVCWLFVYVLE 216

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
           LGVIG     ++SWW+ VF L+ YT  G CPLTWTGFS+++F  LWEF +LS+++G+M+C
Sbjct: 217 LGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFTKLSASSGIMVC 276

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LENWYYR+L+++TGNL++A I VD++S+CMSING EMM+PL FF GT VRVANELGAGNG
Sbjct: 277 LENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSVRVANELGAGNG 336

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           K A+FA I+SV QS +IG++I ++   L D+I ++F++S +V++AV+ LS +L+  ILLN
Sbjct: 337 KRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNLSILLSFAILLN 396

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVAVGSGWQS VA+IN+GCYY IGLPLG +M W+F FGV          GIW 
Sbjct: 397 SVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVK---------GIWA 456

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKWS 506
           GMIFGGT +QT+ILI IT R DW+KEA+ A+  + KWS
Sbjct: 457 GMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Spg033938.1 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 436.4 bits (1121), Expect = 3.1e-122
Identity = 225/458 (49.13%), Postives = 324/458 (70.74%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+E+++LW++  P+IF+R S + ++II+Q+F G LG IELA+ SI  TV++ F+ G+L+
Sbjct: 33  VWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFTVLLRFSNGILL 92

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMASALETLCGQAYGAK  HMLGIYLQRSWIVL  C+  L P Y+++ P+L  +GQ + +
Sbjct: 93  GMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGPILLALGQEERI 152

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
              + ++A+W+I ++FSF   F  Q FLQ Q K ++IA+VA   LGV++  SW+L+    
Sbjct: 153 VRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHVFLSWLLMVHFN 212

Query: 228 LGVIGAAIALDISWWV-LVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLML 287
            G+ GA  +  +++W+  +  L F T GG C  TW GFS+ AF+ LW   +LS ++G ML
Sbjct: 213 FGITGAMTSTLVAFWLPNIAQLLFVTCGG-CKDTWRGFSMMAFKDLWPVFKLSMSSGGML 272

Query: 288 CLENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGN 347
           CLE WY  ILVLLTGNLKNA +A+DAL++C++ING EMMI LGF     VRV+NELG+GN
Sbjct: 273 CLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASVRVSNELGSGN 332

Query: 348 GKGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILL 407
            KGAKFAT+ +V  S  +G+V+  V + L  ++++IFTTS +V   V  LS +LA +IL+
Sbjct: 333 PKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADLSPLLAFSILM 392

Query: 408 NSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIW 467
           NS+QPVLSGVAVG+GWQ +V Y+N+ CYY++G+P+G I+ +V   G+ V        G+W
Sbjct: 393 NSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYV--VGLQVK-------GVW 452

Query: 468 LGMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           +GM+F G  +QT +L ++T RTDWD++   +   L +W
Sbjct: 453 IGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Spg033938.1 vs. TAIR 10
Match: AT1G33100.1 (MATE efflux family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.1e-119
Identity = 218/457 (47.70%), Postives = 321/457 (70.24%), Query Frame = 0

Query: 48  LWLETQQLWLIVGPSIFSRVSAYSMNIITQAFAGRLGDIELASISIANTVIVGFNFGLLV 107
           +W+E+++LW++  P+IF+R S + ++++TQAF G LG  ELA+ SI  T+++ F+ G+L+
Sbjct: 30  VWIESKKLWVVAAPAIFTRYSTFGVSMVTQAFIGHLGPTELAAYSITFTILLRFSNGILL 89

Query: 108 GMASALETLCGQAYGAKMYHMLGIYLQRSWIVLLLCSFLLLPFYVYATPLLKLMGQADDV 167
           GMA AL TLCGQAYGAK Y MLGIYLQRSWIVL   +  L+P +++A P+L  +GQ + +
Sbjct: 90  GMAGALGTLCGQAYGAKQYQMLGIYLQRSWIVLTGGTICLMPVFIFAGPILLALGQEERI 149

Query: 168 AEQSGVVAVWMIPLHFSFAFQFPLQRFLQCQLKTQVIAWVALAGLGVNIVASWVLVYVLE 227
              + V+A+W+I ++FSF   F  Q FLQ Q K ++I++V    LG+++  SW+LV    
Sbjct: 150 VRVARVLALWVIGINFSFVPSFTCQMFLQAQSKNKIISYVTAVSLGLHVFFSWLLVAHFN 209

Query: 228 LGVIGAAIALDISWWVLVFGLYFYTVGGWCPLTWTGFSIQAFQGLWEFIRLSSAAGLMLC 287
            G+ GA  ++ I++W+ +     Y   G C  TW GFS+ AF+ LW  ++LS ++G MLC
Sbjct: 210 FGITGAMTSMLIAFWLPIIVQLLYVTCGGCKDTWRGFSMLAFKDLWPVLKLSLSSGGMLC 269

Query: 288 LENWYYRILVLLTGNLKNATIAVDALSVCMSINGWEMMIPLGFFVGTGVRVANELGAGNG 347
           LE WY  +LVLLTGNLKNA +A+DAL++C+SIN  EMMI LGF     VRV+NELG+GN 
Sbjct: 270 LELWYNSVLVLLTGNLKNAEVALDALAICISINALEMMIALGFLAAVSVRVSNELGSGNP 329

Query: 348 KGAKFATIVSVVQSSVIGVVICIVTMILHDKIAFIFTTSSSVVEAVDTLSNILAITILLN 407
           KGAKFAT+++V  S  IG+V+  V + L  +I++IFTTS +V   V  LS +LA +ILLN
Sbjct: 330 KGAKFATLIAVFTSLSIGIVLFFVFLFLRGRISYIFTTSEAVAAEVADLSPLLAFSILLN 389

Query: 408 SIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLGFIMEWVFHFGVSVNQKSSLHAGIWL 467
           S+QPVLSGVA+G+GWQ +VAY+N+ CYY++G+P+G I+ +V   G+ V        G+W+
Sbjct: 390 SVQPVLSGVAIGAGWQGYVAYVNLACYYLVGIPIGVILGYV--VGLQVK-------GVWI 449

Query: 468 GMIFGGTALQTIILIIITARTDWDKEAKKAQEHLKKW 505
           GM+F G  +QT +L ++T RTDWD++   +  ++ +W
Sbjct: 450 GMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRNINRW 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951012.16.3e-21883.98protein DETOXIFICATION 27-like [Cucurbita moschata][more]
XP_022131676.18.2e-21878.42protein DETOXIFICATION 27-like [Momordica charantia][more]
XP_023537925.11.1e-21780.45protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo][more]
KAG7020273.13.1e-21778.11Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023002321.15.3e-21783.33protein DETOXIFICATION 27-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FKQ11.3e-17065.79Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1[more]
Q1PDX95.8e-16662.58Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1[more]
Q9FNC11.1e-16161.79Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1[more]
Q8W4884.4e-12149.13Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1[more]
F4HPH21.6e-11847.70Protein DETOXIFICATION 20 OS=Arabidopsis thaliana OX=3702 GN=DTX20 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GGG73.1e-21883.98Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453988 PE=3 SV=1[more]
A0A6J1BQ654.0e-21878.42Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1[more]
A0A6J1KL042.6e-21783.33Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111496195 PE=3 SV=1[more]
A0A6J1BQ614.6e-21482.03Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1[more]
A0A6J1BQC75.0e-21377.62Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65380.19.5e-17265.79MATE efflux family protein [more]
AT5G10420.14.1e-16762.58MATE efflux family protein [more]
AT5G44050.18.1e-16361.79MATE efflux family protein [more]
AT1G33110.13.1e-12249.13MATE efflux family protein [more]
AT1G33100.11.1e-11947.70MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 61..453
e-value: 2.0E-70
score: 235.6
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 283..444
e-value: 1.4E-27
score: 96.3
coord: 61..221
e-value: 1.7E-30
score: 105.8
NoneNo IPR availablePANTHERPTHR11206:SF194PROTEIN DETOXIFICATIONcoord: 37..499
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 37..499
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 51..496
e-value: 1.99852E-152
score: 440.086

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg033938Spg033938gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg033938.1-five_prime_utrSpg033938.1-five_prime_utr-scaffold13:34093425..34093635five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34093636..34093953CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34094021..34094562CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34094670..34094756CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34094871..34094927CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34095240..34095478CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34095603..34095748CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34095826..34095915CDS
Spg033938.1-cdsSpg033938.1-cds-scaffold13:34096238..34096282CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg033938.1-three_prime_utrSpg033938.1-three_prime_utr-scaffold13:34096283..34096517three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg033938.1Spg033938.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity