Spg033626.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg033626.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionWAT1-related protein
Locationscaffold13: 38466026 .. 38468706 (+)
Sequence length882
RNA-Seq ExpressionSpg033626.1
SyntenySpg033626.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGCGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCGACCATCTACAGCCCGGCGACGACGGTGATGGTGCAGGGCAGGGCGGAGACGGCGGGGATGCTGACGGGGCCAGGAATGTTCCCGTCACTGGGCGATGCAAAGGGGAAGAGCTGGACCATGGGGTGCGTGTACTTGATCGGGCACTGCCTGTCGTGGTCGGCGTGGCTGGTTCTACAAGCGCCGGTGCTGAAGAAATATCCGGCTCGGCTCTCCGTCACGTCCTTCACTTGCTTCTTTGGCCTCGTCCAATTCTTCATCATCGCCGCCGTGTTCGAGCGCGACGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTCAGTATTGTTTATGCGGTTAGTACAAGTTTTCTGACTTTTTCATATGGTAGATTTTTAGTCTCTTCAACTTTTAAAATATATTTTGGCGCTGTTTGACATATAAATAGAAAAAAAAAAAAATACATTTTTGGTCTTCAGAAAAGAAAAATAAAAAAATAAAATGATTTTAATTAATTTAATATTATTTTAAAAAAAAATTAATAAAAAAAAAATAGTGACACGTCAACCATCCGTGAGTGATTGGAAAATTGAACGGAAGGATCGAAATGAAACGAAATTCAAACTTCAGGACCAAAATGAAACTTGACCAAAATCTCATGGACCAAAAGTATATTTTCCCCTAAAAAATAAATAAGGTGCTTCATAAGTGTTTTCATTTTTCATTTCTCTCTTCTTTTGAGAAATGTTTTCAAAATTGAATTTGAACACAACACAATCAACTTTTTTTATAAATAAAAATATTATTTATCATTGTTGAATAACTTATAAAATGCATAATAATTATCTAAAAATAAATATAACATAAAATTTATGGATATTTAATTTTAAAAGTTAATTATTTTTCAATAAAAAAAATTTAAAAGTTAATTTGTAAATGAAATATTTATATTTTTAATTTAAAACTTCAAAGGACAAGAAGCAAGAAAATTTTCCAAACATCCCCGAGCTTTGTATCTTGTTTTAATTAAAACAATATTTACGGAGGGATCTTTCTATACACCTTATTCAAATCTTGACAACTAAGACAGAAAAATTAAAGGGTTTTTCTTTTTTTTCTTTTTTTTTTTTAAAGTTTTGGTTGGTGGGTTTATTATGTTGGATTTTGAGGAAATGGGGAAATGGATGTGCAGGGGGTGGTGGCTTCGGGGATAGCATTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCTGTCTTTGTTGCTGTCTACCAACCCGTTCAGACCTTCGTCGTTGCTGTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGTGGGTCCTCCTTCTCTCTCTCCTCTCCTTTAATCTACATCCTTCATTCATTCCATCTCATCTGATCGACACACACTCACGAATTTACCATGCCCGTGAATTTTCAAAAATATTGAGAATATTAATGATTTATTAGATACAAAATTAAAAATTTAACAAACGTAATAATACCATGTGGTTTTTTTATTTTTTAAGATTATATTAACAAAATATACATGACTTGAAATATACTTTTAAAAATTCTTAATAAACTATATCCAATATTTGTATTTTGGTTAAATTGCAAGTTTAGTCTCTAAACTTTCGGGATTGTGTCAATTTGGTTCTTGAACTTTAAAAAGTGTCTTACAAGTGTTTGAACTTTCAACTTTATGTCTAATAGGTCTTTAAACTTTAAAAATATATAAAAAGTCCTTAAGCTTTCAATTTTGAATCTAATAGATTTCTGGCTATTTCATATTTTTAATAATTAACCAATCTATTAGATATAAAATTGATTTTCATGTTTATTATTATTATTTTTTTTTTTTTTGACAACATGTGGGAGTGTGAATTGAACCTTCGGCCTCTTGGTCGGAATACAAGCTTTGTGTCAATTGAGTCATGCTCTTGTTGGTTGATTTTCATGTTTATTGGGACCCTAAACTTTCATTCTTGTATAAAGTAGACTTGTGAATTTAAAAAAAATGTTGAATAGGTCAAATACTTAGACATAAATTTGAAAGTTGAGGGATTTATTGAAAACAAAAGTGAAAGTTTAAGGAATTACTAGATAATTTTTAAAGTTCAATAACTTATTGGATATGAAAGTTGAATCACTTATTTGATATATTTTAAAGTTTAGAAAACAAATTAACGCTACCTTAAAACTTGAAACTATTTTTTTTTAAAGTAACTCTCACTCAAAACCCCAACCACACGACTCTTCTTTAATCATAAATTTTGCTGGGCCCTAACATGGCTTCCGTTCAACGGAGAGGTTTGGACACTGGAATTACGCATCCTTATCTTTGACAAGTCAATCTACTAATCTAATTATTAGGTTTAAAAAAATATATAAATATATGTTATTTAAAAGTATTGGGGAGACACATTGATTAAGCATGGGGTTGGATGTACAGGATCATAGGGGCGGTGCTGATTATATCGGGACTGTACCTTGTGCTGTGGGGCAAGAGCGAAGAAAAGAAGCTCTTGATGGAAAGGGCAGTGATCCAGTCTACTCCGGACCACGGTACCAGCAGAACAAGCGCCCACATCAAGCCGTCCCTGAATCAGCCACTCCTCCATCCTACGGCTGAAAATGTTTGA

mRNA sequence

ATGCTGCGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCGACCATCTACAGCCCGGCGACGACGGTGATGGTGCAGGGCAGGGCGGAGACGGCGGGGATGCTGACGGGGCCAGGAATGTTCCCGTCACTGGGCGATGCAAAGGGGAAGAGCTGGACCATGGGGTGCGTGTACTTGATCGGGCACTGCCTGTCGTGGTCGGCGTGGCTGGTTCTACAAGCGCCGGTGCTGAAGAAATATCCGGCTCGGCTCTCCGTCACGTCCTTCACTTGCTTCTTTGGCCTCGTCCAATTCTTCATCATCGCCGCCGTGTTCGAGCGCGACGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTCAGTATTGTTTATGCGGAAATGGGGAAATGGATGTGCAGGGGGTGGTGGCTTCGGGGATAGCATTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCTGTCTTTGTTGCTGTCTACCAACCCGTTCAGACCTTCGTCGTTGCTGTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGTGGGTCCTCCTTCTCTCTCTCCTCTCCTTTAATCTACATCCTTCATTCATTCCATCTCATCTGATCGACACACACTCACGAATTTACCATGCCCAGGTTTGGACACTGGAATTACGCATCCTTATCTTTGACAAGATCATAGGGGCGGTGCTGATTATATCGGGACTGTACCTTGTGCTGTGGGGCAAGAGCGAAGAAAAGAAGCTCTTGATGGAAAGGGCAGTGATCCAGTCTACTCCGGACCACGGTACCAGCAGAACAAGCGCCCACATCAAGCCGTCCCTGAATCAGCCACTCCTCCATCCTACGGCTGAAAATGTTTGA

Coding sequence (CDS)

ATGCTGCGTGGCCGGAGCGACGGTGATCACATTATACAAAGGGCCGACCATCTACAGCCCGGCGACGACGGTGATGGTGCAGGGCAGGGCGGAGACGGCGGGGATGCTGACGGGGCCAGGAATGTTCCCGTCACTGGGCGATGCAAAGGGGAAGAGCTGGACCATGGGGTGCGTGTACTTGATCGGGCACTGCCTGTCGTGGTCGGCGTGGCTGGTTCTACAAGCGCCGGTGCTGAAGAAATATCCGGCTCGGCTCTCCGTCACGTCCTTCACTTGCTTCTTTGGCCTCGTCCAATTCTTCATCATCGCCGCCGTGTTCGAGCGCGACGCTCAGGCTTGGCTCTTCCACTCCGGCGCCGAAGCCTTCAGTATTGTTTATGCGGAAATGGGGAAATGGATGTGCAGGGGGTGGTGGCTTCGGGGATAGCATTTGCTGTACAGATATGGTGCATTGACAGAGGTGGCCCTGTCTTTGTTGCTGTCTACCAACCCGTTCAGACCTTCGTCGTTGCTGTCATGGCTTCCTTCGCTTTGGGCGAGGAGTTCTACTTGGGAGGGTGGGTCCTCCTTCTCTCTCTCCTCTCCTTTAATCTACATCCTTCATTCATTCCATCTCATCTGATCGACACACACTCACGAATTTACCATGCCCAGGTTTGGACACTGGAATTACGCATCCTTATCTTTGACAAGATCATAGGGGCGGTGCTGATTATATCGGGACTGTACCTTGTGCTGTGGGGCAAGAGCGAAGAAAAGAAGCTCTTGATGGAAAGGGCAGTGATCCAGTCTACTCCGGACCACGGTACCAGCAGAACAAGCGCCCACATCAAGCCGTCCCTGAATCAGCCACTCCTCCATCCTACGGCTGAAAATGTTTGA

Protein sequence

MLRGRSDGDHIIQRADHLQPGDDGDGAGQGGDGGDADGARNVPVTGRCKGEELDHGVRVLDRALPVVVGVAGSTSAGAEEISGSALRHVLHLLLWPRPILHHRRRVRARRSGLALPLRRRSLQYCLCGNGEMDVQGVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLSFNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKLLMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV
Homology
BLAST of Spg033626.1 vs. NCBI nr
Match: XP_023537133.1 (protein WALLS ARE THIN 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537134.1 protein WALLS ARE THIN 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-44
Identity = 108/158 (68.35%), Postives = 110/158 (69.62%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 278 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 337

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKKL
Sbjct: 338 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKL 390

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+E AVIQSTPDH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 398 LLEWAVIQSTPDHVTSRTSGHIKPSLNQPLLHPTAENV 390

BLAST of Spg033626.1 vs. NCBI nr
Match: XP_022951323.1 (protein WALLS ARE THIN 1-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 5.3e-44
Identity = 107/158 (67.72%), Postives = 110/158 (69.62%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEE+KL
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEERKL 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+E AVIQSTPDH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 401 LLEWAVIQSTPDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Spg033626.1 vs. NCBI nr
Match: KAG7020479.1 (Protein WALLS ARE THIN 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 188.3 bits (477), Expect = 9.1e-44
Identity = 107/158 (67.72%), Postives = 109/158 (68.99%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKKL
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKL 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+E AVIQS PDH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 401 LLEWAVIQSAPDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Spg033626.1 vs. NCBI nr
Match: KAG6585565.1 (Protein WALLS ARE THIN 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 188.3 bits (477), Expect = 9.1e-44
Identity = 107/158 (67.72%), Postives = 109/158 (68.99%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKKL
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKL 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+E AVIQS PDH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 401 LLEWAVIQSAPDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Spg033626.1 vs. NCBI nr
Match: XP_022997349.1 (protein WALLS ARE THIN 1-like [Cucurbita maxima])

HSP 1 Score: 186.0 bits (471), Expect = 4.5e-43
Identity = 104/158 (65.82%), Postives = 108/158 (68.35%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKK 
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKF 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+ERAVIQS PDHGT R S HIKPSLNQPLLHPTA++V
Sbjct: 401 LLERAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Spg033626.1 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-31
Identity = 83/162 (51.23%), Postives = 95/162 (58.64%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+VASGIAFAVQIWCIDRGGPVFVAVYQPVQT VVA+MAS ALGEEFYLGG         
Sbjct: 273 GIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYLGG--------- 332

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLII+GLY VL+GKSEE+K 
Sbjct: 333 ------------------------------------IIGAVLIIAGLYFVLYGKSEERKF 389

Query: 256 -LMERAVIQSTPDHGTSRTSA---HIKPSLNQPLLHPTAENV 294
             +E+A IQS+ +HG  R       IK S+  PLLH + +NV
Sbjct: 393 AALEKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Spg033626.1 vs. ExPASy Swiss-Prot
Match: Q6J163 (Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-23
Identity = 78/169 (46.15%), Postives = 90/169 (53.25%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G VASGIAF+VQIWCIDRGGPVFVAVYQPVQT  VA+MAS  LGE+FYLGG         
Sbjct: 272 GFVASGIAFSVQIWCIDRGGPVFVAVYQPVQTIAVAIMASIILGEQFYLGG--------- 331

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               I GA+LII GLYLVLWGKSEEK+L
Sbjct: 332 ------------------------------------IFGAILIIIGLYLVLWGKSEEKRL 391

Query: 256 LMERA----VIQSTPDHGTS------RTSAHIKP----SLNQPLLHPTA 291
            + +A    V ++ PD+          +S  IKP    SL QPLL  T+
Sbjct: 392 GLLQAKSSMVPENQPDNMDQSATLIINSSNGIKPNTSSSLTQPLLLDTS 395

BLAST of Spg033626.1 vs. ExPASy Swiss-Prot
Match: Q9LV20 (WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.1e-14
Identity = 47/125 (37.60%), Postives = 63/125 (50.40%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G++ASG+   +Q WCI + GPVFVAV+QP+QT +VA MA   LG++ Y GG         
Sbjct: 279 GIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTLLVAAMAFLILGDQLYSGG--------- 338

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               I+GAV I+ GLYLVLWGK+EE+KL
Sbjct: 339 ------------------------------------IVGAVFIMLGLYLVLWGKNEERKL 358

Query: 256 LMERA 261
            +E +
Sbjct: 399 ALEES 358

BLAST of Spg033626.1 vs. ExPASy Swiss-Prot
Match: F4J9A3 (WAT1-related protein At3g53210 OS=Arabidopsis thaliana OX=3702 GN=At3g53210 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.7e-13
Identity = 52/156 (33.33%), Postives = 76/156 (48.72%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+V S + FA+QI+ ++RGGP+FV+ Y P+QT + AV+A+ ALGE FYLGG         
Sbjct: 257 GLVGSAMVFAIQIYVVERGGPLFVSAYLPLQTLIAAVLATLALGEHFYLGG--------- 316

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               +IGA+LI+SGLYLV+ GKS E + 
Sbjct: 317 ------------------------------------LIGAILIMSGLYLVVMGKSWENQA 367

Query: 256 L---MERAVIQSTPDHGTSRTSAHIKP--SLNQPLL 287
           L    +  +  +  D G      + KP   ++QPL+
Sbjct: 377 LCQQQQHMISSAASDFGDEEDYHNNKPRSPISQPLI 367

BLAST of Spg033626.1 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-07
Identity = 42/130 (32.31%), Postives = 58/130 (44.62%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+VAS I++ VQ   + + GPVF   + P+   +VAVM SF L E+ +LGG         
Sbjct: 272 GIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLGG--------- 331

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               +IGAVLI+ GLY VLWGK +E ++
Sbjct: 332 ------------------------------------VIGAVLIVIGLYAVLWGKQKENQV 356

Query: 256 -LMERAVIQS 265
            + E A I S
Sbjct: 392 TICELAKIDS 356

BLAST of Spg033626.1 vs. ExPASy TrEMBL
Match: A0A6J1GHE4 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111454189 PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 2.6e-44
Identity = 107/158 (67.72%), Postives = 110/158 (69.62%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEE+KL
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEERKL 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+E AVIQSTPDH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 401 LLEWAVIQSTPDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Spg033626.1 vs. ExPASy TrEMBL
Match: A0A6J1KNR5 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111496234 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.2e-43
Identity = 106/158 (67.09%), Postives = 108/158 (68.35%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MAS ALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASLALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKKL
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKL 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+ERAVIQS  DH TSRTS HIKPSLNQPLLHPTAENV
Sbjct: 401 LLERAVIQSAQDHVTSRTSGHIKPSLNQPLLHPTAENV 393

BLAST of Spg033626.1 vs. ExPASy TrEMBL
Match: A0A6J1KB58 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111492292 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.2e-43
Identity = 104/158 (65.82%), Postives = 108/158 (68.35%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKK 
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKF 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+ERAVIQS PDHGT R S HIKPSLNQPLLHPTA++V
Sbjct: 401 LLERAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Spg033626.1 vs. ExPASy TrEMBL
Match: A0A6J1HEL9 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462346 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.2e-43
Identity = 104/158 (65.82%), Postives = 108/158 (68.35%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVA+MASFALGEEFYLGG         
Sbjct: 281 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVALMASFALGEEFYLGG--------- 340

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKK 
Sbjct: 341 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKF 393

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+ERAVIQS PDHGT R S HIKPSLNQPLLHPTA++V
Sbjct: 401 LLERAVIQSVPDHGTRRPSGHIKPSLNQPLLHPTADSV 393

BLAST of Spg033626.1 vs. ExPASy TrEMBL
Match: A0A6J1CR33 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111013973 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-41
Identity = 101/158 (63.92%), Postives = 107/158 (67.72%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGG         
Sbjct: 280 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGG--------- 339

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLIISGLYLVLWGKSEEKK 
Sbjct: 340 ------------------------------------IIGAVLIISGLYLVLWGKSEEKKF 392

Query: 256 LMERAVIQSTPDHGTSRTSAHIKPSLNQPLLHPTAENV 294
           L+ RAVIQS P+H ++RT+AHIKPSL  PLLHP+AENV
Sbjct: 400 LLARAVIQSAPEHSSNRTTAHIKPSLTAPLLHPSAENV 392

BLAST of Spg033626.1 vs. TAIR 10
Match: AT1G75500.1 (Walls Are Thin 1 )

HSP 1 Score: 136.0 bits (341), Expect = 5.0e-32
Identity = 83/162 (51.23%), Postives = 95/162 (58.64%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+VASGIAFAVQIWCIDRGGPVFVAVYQPVQT VVA+MAS ALGEEFYLGG         
Sbjct: 273 GIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYLGG--------- 332

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLII+GLY VL+GKSEE+K 
Sbjct: 333 ------------------------------------IIGAVLIIAGLYFVLYGKSEERKF 389

Query: 256 -LMERAVIQSTPDHGTSRTSA---HIKPSLNQPLLHPTAENV 294
             +E+A IQS+ +HG  R       IK S+  PLLH + +NV
Sbjct: 393 AALEKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Spg033626.1 vs. TAIR 10
Match: AT1G75500.2 (Walls Are Thin 1 )

HSP 1 Score: 136.0 bits (341), Expect = 5.0e-32
Identity = 83/162 (51.23%), Postives = 95/162 (58.64%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+VASGIAFAVQIWCIDRGGPVFVAVYQPVQT VVA+MAS ALGEEFYLGG         
Sbjct: 273 GIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIMASIALGEEFYLGG--------- 332

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               IIGAVLII+GLY VL+GKSEE+K 
Sbjct: 333 ------------------------------------IIGAVLIIAGLYFVLYGKSEERKF 389

Query: 256 -LMERAVIQSTPDHGTSRTSA---HIKPSLNQPLLHPTAENV 294
             +E+A IQS+ +HG  R       IK S+  PLLH + +NV
Sbjct: 393 AALEKAAIQSSAEHGIERAPVSRNSIKSSITTPLLHQSTDNV 389

BLAST of Spg033626.1 vs. TAIR 10
Match: AT3G18200.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-15
Identity = 47/125 (37.60%), Postives = 63/125 (50.40%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G++ASG+   +Q WCI + GPVFVAV+QP+QT +VA MA   LG++ Y GG         
Sbjct: 256 GIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTLLVAAMAFLILGDQLYSGG--------- 315

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               I+GAV I+ GLYLVLWGK+EE+KL
Sbjct: 316 ------------------------------------IVGAVFIMLGLYLVLWGKNEERKL 335

Query: 256 LMERA 261
            +E +
Sbjct: 376 ALEES 335

BLAST of Spg033626.1 vs. TAIR 10
Match: AT3G18200.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-15
Identity = 47/125 (37.60%), Postives = 63/125 (50.40%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G++ASG+   +Q WCI + GPVFVAV+QP+QT +VA MA   LG++ Y GG         
Sbjct: 149 GIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTLLVAAMAFLILGDQLYSGG--------- 208

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               I+GAV I+ GLYLVLWGK+EE+KL
Sbjct: 209 ------------------------------------IVGAVFIMLGLYLVLWGKNEERKL 228

Query: 256 LMERA 261
            +E +
Sbjct: 269 ALEES 228

BLAST of Spg033626.1 vs. TAIR 10
Match: AT3G53210.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-14
Identity = 52/156 (33.33%), Postives = 76/156 (48.72%), Query Frame = 0

Query: 136 GVVASGIAFAVQIWCIDRGGPVFVAVYQPVQTFVVAVMASFALGEEFYLGGWVLLLSLLS 195
           G+V S + FA+QI+ ++RGGP+FV+ Y P+QT + AV+A+ ALGE FYLGG         
Sbjct: 257 GLVGSAMVFAIQIYVVERGGPLFVSAYLPLQTLIAAVLATLALGEHFYLGG--------- 316

Query: 196 FNLHPSFIPSHLIDTHSRIYHAQVWTLELRILIFDKIIGAVLIISGLYLVLWGKSEEKKL 255
                                               +IGA+LI+SGLYLV+ GKS E + 
Sbjct: 317 ------------------------------------LIGAILIMSGLYLVVMGKSWENQA 367

Query: 256 L---MERAVIQSTPDHGTSRTSAHIKP--SLNQPLL 287
           L    +  +  +  D G      + KP   ++QPL+
Sbjct: 377 LCQQQQHMISSAASDFGDEEDYHNNKPRSPISQPLI 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023537133.12.4e-4468.35protein WALLS ARE THIN 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537... [more]
XP_022951323.15.3e-4467.72protein WALLS ARE THIN 1-like [Cucurbita moschata][more]
KAG7020479.19.1e-4467.72Protein WALLS ARE THIN 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6585565.19.1e-4467.72Protein WALLS ARE THIN 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022997349.14.5e-4365.82protein WALLS ARE THIN 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94AP37.0e-3151.23Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1[more]
Q6J1631.4e-2346.15Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1[more]
Q9LV202.1e-1437.60WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2... [more]
F4J9A31.7e-1333.33WAT1-related protein At3g53210 OS=Arabidopsis thaliana OX=3702 GN=At3g53210 PE=2... [more]
Q9FL411.4e-0732.31WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1GHE42.6e-4467.72WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111454189 PE=3 SV=1[more]
A0A6J1KNR52.2e-4367.09WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111496234 PE=3 SV=1[more]
A0A6J1KB582.2e-4365.82WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111492292 PE=3 SV=1[more]
A0A6J1HEL92.2e-4365.82WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111462346 PE=3 SV=1[more]
A0A6J1CR331.6e-4163.92WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111013973 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G75500.15.0e-3251.23Walls Are Thin 1 [more]
AT1G75500.25.0e-3251.23Walls Are Thin 1 [more]
AT3G18200.11.5e-1537.60nodulin MtN21 /EamA-like transporter family protein [more]
AT3G18200.21.5e-1537.60nodulin MtN21 /EamA-like transporter family protein [more]
AT3G53210.11.2e-1433.33nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..47
NoneNo IPR availablePANTHERPTHR31218:SF322WAT1-RELATED PROTEINcoord: 231..280
NoneNo IPR availablePANTHERPTHR31218:SF322WAT1-RELATED PROTEINcoord: 135..188
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 134..189
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 231..280
coord: 135..188

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg033626Spg033626gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg033626.1-cdsSpg033626.1-cds-scaffold13:38466026..38466407CDS
Spg033626.1-cdsSpg033626.1-cds-scaffold13:38467221..38467490CDS
Spg033626.1-cdsSpg033626.1-cds-scaffold13:38468373..38468412CDS
Spg033626.1-cdsSpg033626.1-cds-scaffold13:38468517..38468706CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg033626.1Spg033626.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity