Spg033212.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg033212.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold5: 1060591 .. 1064201 (+)
Sequence length	1068
RNA-Seq Expression	Spg033212.1
Synteny	Spg033212.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAATCTTTACATATCTGAAGAATAATTAGATAAAGATAAAGAAAAAAAATGAGCTGTCTGCAGAGTTGGCCGGAGCCGGTGGTGTGCGTGCAGTCGCTGGCGGAGAGCGGAATAAGGACGATTCCGGGGAGGTACGTGAAGCCGCCGTCGCAGAGGCCGAGTGGGGCCGCCGTGGAGAGTGAGGTGCGGAGGAACATCCCCGTGGTGGACATGGAGAAGCTGCAGAGCGGGGCGGCGGTGAGGGAGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTCCACGTCATAAACCATGGGATTAGCGGCGAGATGATGGAATGTGTGAAGGACACGTGGCGGAAGTTCTTCGATCAGCCGTTGGATGTGAAGAAGCAATATGCAAATTCGCCGTCGACTTATGAAGGATACGGCAGCCGATTGGGGATCGAAAAAGGGGCGATTTTGGATTGGAGTGATTACTTTTTCTTGAATTTTATGCCTATTTCCCACAGAAACCCTGCCAAGTGGCCTGCTTTTCCTCCATCCTCCAAGTAGGTTTACCTATTCTCCTTATTTCTCGACTTTTTTTTTATCCCGCTCGATACTTGTCTACACATCGATCACTAAGAATTCTCTGATGTAGCGTATTTTCATTGGGTGTGATTAATTCTATTATATTTAATAGATAAGCGGCTCTGTTCCGCTAAAAATTTTAAGCCATTGAAAATGCACCACATCAGAAAGTCAGGATGAGCTCCTACCGATTGGTGGGTAGATGAGTTTTTTTCCCTCATTTGCTGTTTGCTTATACATTGAGTAGTAAGAGCATTTTTATTGGTTTTAATTAATTCTATTACATGGATGAGTGAGTTTTACGGGAATTTTTTAGCCATTAAAAGTGCACCACATCACGGAATTATTTTTTTTTTTTACATGATGATTCTAATTGACTTTTTTGATGTAATATATATTTTTAATGGCTTAAAATTTGTAGTGGAACTTGTTTATACGACGTATCAAAAAGTTGGTAATGACAACTTTAAAATTTTAAGGGGGTAAATTGGAGAGAAGACTTTAAACATCTTAGTTTTTTTCAATTTGAGATGAGCTAGAGGGAGCGACTGTCTTCCTTCTTTTGACGAGGATATCGTCTTAATAAAGTATCCTTCTTTGTCTTCTCATCTTTCTCCTTTTTAATATATGGTTTAAGGGATGTGTTTGCTTGTTTTTTTTTTTTTTTTTTCCTTACAAGTCCTTTTGGTTGATTTTTCGACTGCAAGAATAATGGTTCTTGTCGACGATCTTCCACCATGAATATATTAGAAACCTTGAACGTCCTCAAAAGTTTAATATGGAAACTTTTTTATTTTTCTGAAATTAGGGTTTAATTTAAAGTCTGTTGTGAAGTTGAACCTTTCGAGCTTCCAAAACAATTTCTTCTTTTTGCTATGAAAACTATATTTAATTACCTGTTATGGAAGTACACATTAAGTAGTATATAAATAAAAACTAATAATATATATATTACTTATTTTGGGGGTTAATTTAGTTGTTAATAAGTCACTGTGAAAGTACTCTTATGGTTTGTTTGGTACCAATCTTTTTCCTACAAAAGTTAGTCATTTTCGGGGGTTGTCGGAAGTGGTAAAATAATGTACAATTTTGTACTGCATATAAACCCCTTTTTTACCCCTAACTTTAATTCCCATGTAAAATCTCTTCAAAAAAAAAAAAAAAAATGTAAAAACAATATTTATATAGAAATCACAAACCAAAGTTATAAATTGTTTTTCTTTTTTCTTTTTTTTTTTTAAAAAAATATGATTCTCAACCTTCTTTTTCTTGTATATTAATGATTTTAATTCTCAAGGTTAAATAATTTTTATTTTATTCTTCCTCCAATGTTCTTGGATCCACCCCTAAATGCTAATTAATTTATCTATGTTATTTTAGTCTCAATTCATATATCTTACATTTCCATGAACATAGACTCACCCAATTCTATAGACTCGTCCATCTAAATATATTGCTCAATTGATTAAAACGTTATACAAATCCCTACTCCAACATGTTATTGTTGAACCAAAAAATAAATCTATGATAACAAAAAAGAAAAATAAAAAACAATATTTCCCACCTCGACTTTCTTATCATATCGTCACATGAATAACCAATACCCATTTGATAAAACCGATACTTTTACACCGTTTTAGGGTTTTATATGCTAACGAAAGAACGGTTTTGTTGTTGTGGGCAGGGAATTGATAGAAGAATATGGGAATGAGGTAATGAAGCTATGTGGGAAAGTGATGAAGGGATTATCAAAGGGTTTAGGGCTTGAAGAAGAGTATTTATTGAAGGCATTTGGAGGAGAAAATGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCAAAGTGCCCACAGCCAGACCTCACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGCAAGGGAGATGATTGGATCACCATTGACCCTATCCCTAATGCTTTGATCGTCAACGTCGGTGATCAAATTCAGGTCGGTTCGATCTTCTTCTTCTAGGGTTCGAAACCCTACATGGTCGAGTTTGATCGTCAACCATGTAGGGTTGGTTGATCTTTTTCGATTATTAGGGCAAATTTCACATGTTTCCATCTCCTTTTTCTTTACCCTAAGTCACGATTAGTATCTCATGTCGATTGAAAATTTAGAATATTTGTTGTAAATTTACAAATTACTTTAGGTTATATGTTAAACGTTTATTGGATTTTAAACTTTAAAGCCTTGGAAAATTAGTCTGAAACTCGAGAAATGAGCTATTGGGCTATTAAACTTAATATATTTTGAGTTTAAATTGATAGGTTGTGATATATTTGATGTTTTTTATATACTCGTGACAATCACTTATGGTCGTTTGTTTCCTTTTGTTAGGTGCTAAGCAATGCAATATACAAAAGCGTGAAGCATAGGGTGATCGTAAATTCAAATATGGATCGAGTGTCGTTGGCCTTTTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTGACTGAGGATAGGCCAGCCCTTTTTCCACCCATGACCTTTGATGAATATAGACTTTACATTAGGAAGAGGGGTATTTGTGGAACTTCACAACAAGATCAACAATTTCTTAAATCCACTTAATTAGAATTAACAATGTGTTAATGCTTGGTAGTAGTGTGGGGTTTTAAATTTTATTCTAAGTTATTGTTAAAGTTTTTTCTTTCTTTTTTTTTTCTTTTGTTTTTCTTTGGGTTCTTTTGATTCCTTTGTGTGGTCTCAAATGTACCAATGTACCAATTTGCAATTGTCAATGTTAGAATCTGTTCCCCTTTGTCATGATGTGAAAGAACTTGAATATGATCAGTTTTCACTCTCTCTTGTCATCCTCAAAAAGAAAAAAAAAAAAAATTGTGATCAGATTCTCTCCCTGCATGAACAGAAGTTTTGGACTCCTCGAACATCGACACGACCCCAACGAAACATATCTTAAATGTGATAAAAAGAAGCATACAAAATTTCAATATCTTAGGTCCCG

mRNA sequence

ATGAGCTGTCTGCAGAGTTGGCCGGAGCCGGTGGTGTGCGTGCAGTCGCTGGCGGAGAGCGGAATAAGGACGATTCCGGGGAGGTACGTGAAGCCGCCGTCGCAGAGGCCGAGTGGGGCCGCCGTGGAGAGTGAGGTGCGGAGGAACATCCCCGTGGTGGACATGGAGAAGCTGCAGAGCGGGGCGGCGGTGAGGGAGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTCCACGTCATAAACCATGGGATTAGCGGCGAGATGATGGAATGTGTGAAGGACACGTGGCGGAAGTTCTTCGATCAGCCGTTGGATGTGAAGAAGCAATATGCAAATTCGCCGTCGACTTATGAAGGATACGGCAGCCGATTGGGGATCGAAAAAGGGGCGATTTTGGATTGGAGTGATTACTTTTTCTTGAATTTTATGCCTATTTCCCACAGAAACCCTGCCAAGTGGCCTGCTTTTCCTCCATCCTCCAAGGAATTGATAGAAGAATATGGGAATGAGGTAATGAAGCTATGTGGGAAAGTGATGAAGGGATTATCAAAGGGTTTAGGGCTTGAAGAAGAGTATTTATTGAAGGCATTTGGAGGAGAAAATGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCAAAGTGCCCACAGCCAGACCTCACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGCAAGGGAGATGATTGGATCACCATTGACCCTATCCCTAATGCTTTGATCGTCAACGTCGGTGATCAAATTCAGGTGCTAAGCAATGCAATATACAAAAGCGTGAAGCATAGGGTGATCGTAAATTCAAATATGGATCGAGTGTCGTTGGCCTTTTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTGACTGAGGATAGGCCAGCCCTTTTTCCACCCATGACCTTTGATGAATATAGACTTTACATTAGGAAGAGGGGTATTTGTGGAACTTCACAACAAGATCAACAATTTCTTAAATCCACTTAA

Coding sequence (CDS)

ATGAGCTGTCTGCAGAGTTGGCCGGAGCCGGTGGTGTGCGTGCAGTCGCTGGCGGAGAGCGGAATAAGGACGATTCCGGGGAGGTACGTGAAGCCGCCGTCGCAGAGGCCGAGTGGGGCCGCCGTGGAGAGTGAGGTGCGGAGGAACATCCCCGTGGTGGACATGGAGAAGCTGCAGAGCGGGGCGGCGGTGAGGGAGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTCCACGTCATAAACCATGGGATTAGCGGCGAGATGATGGAATGTGTGAAGGACACGTGGCGGAAGTTCTTCGATCAGCCGTTGGATGTGAAGAAGCAATATGCAAATTCGCCGTCGACTTATGAAGGATACGGCAGCCGATTGGGGATCGAAAAAGGGGCGATTTTGGATTGGAGTGATTACTTTTTCTTGAATTTTATGCCTATTTCCCACAGAAACCCTGCCAAGTGGCCTGCTTTTCCTCCATCCTCCAAGGAATTGATAGAAGAATATGGGAATGAGGTAATGAAGCTATGTGGGAAAGTGATGAAGGGATTATCAAAGGGTTTAGGGCTTGAAGAAGAGTATTTATTGAAGGCATTTGGAGGAGAAAATGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCAAAGTGCCCACAGCCAGACCTCACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGCAAGGGAGATGATTGGATCACCATTGACCCTATCCCTAATGCTTTGATCGTCAACGTCGGTGATCAAATTCAGGTGCTAAGCAATGCAATATACAAAAGCGTGAAGCATAGGGTGATCGTAAATTCAAATATGGATCGAGTGTCGTTGGCCTTTTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTGACTGAGGATAGGCCAGCCCTTTTTCCACCCATGACCTTTGATGAATATAGACTTTACATTAGGAAGAGGGGTATTTGTGGAACTTCACAACAAGATCAACAATTTCTTAAATCCACTTAA

Protein sequence

MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSGAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFLKST

Homology

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BLAST of Spg033212.1 vs. NCBI nr
Match: XP_038903719.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 651.7 bits (1680), Expect = 3.5e-183
Identity = 314/354 (88.70%), Postives = 329/354 (92.94%), Query Frame = 0


Query: 2   SCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSG 61
           SCLQSWPEPVVCVQSLAESGIRTIP RYVKPPSQRP G  V+    RNIPVVDMEKL+SG
Sbjct: 3   SCLQSWPEPVVCVQSLAESGIRTIPMRYVKPPSQRPGGDGVQ---MRNIPVVDMEKLESG 62

Query: 62  AAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYG 121
           AAVRE AEAC+EWGFF +INHGISGEMMECVKDTW++FFDQPLD+KKQYANSP+TYEGYG
Sbjct: 63  AAVRETAEACREWGFFQLINHGISGEMMECVKDTWKEFFDQPLDLKKQYANSPATYEGYG 122

Query: 122 SRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKG 181
           SRLGIEKGAILDWSDYFFLNFMP S RNPA WPAFPPSSK+LIEEYG+EVMKLCGK+MKG
Sbjct: 123 SRLGIEKGAILDWSDYFFLNFMPFSLRNPAIWPAFPPSSKKLIEEYGDEVMKLCGKLMKG 182

Query: 182 LSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 241
           LS GLGLEEEYLLKAFG E GIG+CMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 183 LSLGLGLEEEYLLKAFGEEKGIGSCMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 242

Query: 242 VPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFY 301
           VPGLQV KG DWITIDPIPNALIVNVGDQIQVLSN IYKSVKHRVIVNSN DRVSLAFFY
Sbjct: 243 VPGLQVLKGHDWITIDPIPNALIVNVGDQIQVLSNGIYKSVKHRVIVNSNKDRVSLAFFY 302

Query: 302 NPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFLKST 356
           NP+SDLIIEPAKELVTE+ PALFPPMTFDEYRLYIRK+GICGTSQ  QQ  KST
Sbjct: 303 NPKSDLIIEPAKELVTEETPALFPPMTFDEYRLYIRKKGICGTSQH-QQSPKST 352

[Close]

BLAST of Spg033212.1 vs. NCBI nr
Match: KAG6596343.1 (putative 2-oxoglutarate-dependent dioxygenase ANS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.9 bits (1670), Expect = 5.1e-182
Identity = 309/349 (88.54%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIP RYVKPPSQRPSGAAVE  V RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGY 120
            A V+ MAEACKEWGFFHVINHGI+GEMMEC+KD W +FF QPLD+K+QYANSP+TYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMK 180
           GSRLGIEK AILDWSDYFF+N++P+S RN AKWPAFPP SKELIEEYG E++KLC KVMK
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMK 180

Query: 181 GLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240
           GLS GLGLEEEYLLKAFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH
Sbjct: 181 GLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240

Query: 241 NVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFF 300
           NVPGLQVRKGDDWITIDPIPNALIVNV DQIQVLSN IYKSV HRVIVNSN DRVSLAFF
Sbjct: 241 NVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 300

Query: 301 YNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           YNPRSDLIIEPAK LVTE+RPALFPPMTFDEYRL IRKRGICGTSQQ+Q
Sbjct: 301 YNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTSQQEQ 348

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BLAST of Spg033212.1 vs. NCBI nr
Match: XP_022948721.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita moschata])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 308/349 (88.25%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIP RYVKPPSQRPSGAAVE  V RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGY 120
            A V+ MAEACKEWGFFHVINHGI+GEMMEC+KD W +FF QPLD+K+QYANSP+TYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMK 180
           GSRLGIEK AILDWSDYFF+N++P+S RN AKWPAFPP SKELIEEYG E++KLC KVMK
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMK 180

Query: 181 GLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240
           GLS GLGLEEEYLLKAFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH
Sbjct: 181 GLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240

Query: 241 NVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFF 300
           NVPGLQVRKGDDWITIDPIPNALIVNV DQIQVLSN IYKSV HRVIVNSN DRVSLAFF
Sbjct: 241 NVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 300

Query: 301 YNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           YNPRSDLIIEPAK LVTE+RPALFPPMTFDEYRL IR+RGICGTSQQ+Q
Sbjct: 301 YNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQ 348

[Close]

BLAST of Spg033212.1 vs. NCBI nr
Match: XP_023539287.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.6 bits (1664), Expect = 2.5e-181
Identity = 307/349 (87.97%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQS 60
           MSCL+SWPEPVVCVQSLAESGIRTIP RYVKPPSQRPSGAAVE  V RNIPVVDMEKLQS
Sbjct: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGY 120
            A V  MAEACKEWGFFHVINHGI+GEMMEC+KD W +FF QPLD+K+QYANSP+TYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMK 180
           GSRLGIEK AILDWSDYFF+N++P+S RN AKWPAFPP SKELIEEYG E++KLC KVMK
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMK 180

Query: 181 GLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240
           GLS GLGLEEEYLLKAFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH
Sbjct: 181 GLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240

Query: 241 NVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFF 300
           NVPGLQVRKGDDWITIDPIPNALIVN+ DQIQVLSN IYKSV HRVIVNSN DRVSLAFF
Sbjct: 241 NVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 300

Query: 301 YNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           YNPRSDLIIEPAK LVTE+RPALFPP TFDEYRL IRKRGICGTSQQ+Q
Sbjct: 301 YNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQ 348

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BLAST of Spg033212.1 vs. NCBI nr
Match: XP_023528714.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.2 bits (1663), Expect = 3.3e-181
Identity = 308/351 (87.75%), Postives = 330/351 (94.02%), Query Frame = 0


Query: 6   SWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVES-EVRRNIPVVDMEKLQSGAAV 65
           SWPEPVVCVQSLAESGIRTIP RYVKPP QRP GAA+ES EV+RNIPVVDM KL+S AAV
Sbjct: 2   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 61

Query: 66  REMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRL 125
           REMAEAC+EWGFFHVINHGISGE+MECVK++W++FFDQPLD+KKQYANSP TYEGYGSRL
Sbjct: 62  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSK 185
           GIEKGAILDWSDYFFLN+MP S RNPA WPAFPPSSK+LIEEYGNE +KLCGK+MK LS 
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGNEAVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPR 305
           LQVRKGD WITIDPIPNALIVN+GDQIQVLSNAIYKSVKHRVIVNSN DRVSLAFFYNP+
Sbjct: 242 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFLKST 356
           SDLIIEPAKELV+E+RPALFP MTFDEYRL+IRKRGICGTSQ +Q  L+ST
Sbjct: 302 SDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQS-LEST 351

[Close]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 3.2e-126
Identity = 207/347 (59.65%), Postives = 266/347 (76.66%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKL-QSGAAVR 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     +S+    IPV+DM  +      +R
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  EMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRLG 126
            +  AC+EWGFF ++NHG++  +ME V+  WR+FF+ PL+ K++YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSKG 186
           + K A LDWSDYFFLN++P S RNP+KWP+ PP  +ELIE+YG EV KLC ++ + LS+ 
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVN+GDQ+Q+LSN IYKSV+H+VIVNS M+RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFL 353
           D+ + P +ELVT +RPAL+ P+ FDEYR  IR++G CG +Q D   L
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQVDSLLL 351

[Close]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-123
Identity = 200/346 (57.80%), Postives = 271/346 (78.32%), Query Frame = 0


Query: 5   QSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESE---VRRNIPVVDMEKLQSG 64
           + WPEP+V VQSLAES + ++P RY+KPPSQRP    ++ +      NIP++D++ L SG
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AA--VREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEG 124
                + ++EAC+EWGFF VINHG+  E+M+  ++TW+ FF+ P++ K+ Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVM 184
           YGSRLG+EKGAILDW+DY++L+F+P++ ++  KWP+ P + +E+ +EYG E++KL G++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVN+GDQIQ+LSN+ YKSV+HRVIVNS  +RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTS 346
           FYNP+SD+ I+P ++LVT   P L+PPMTFD+YRL+IR +G  G S
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKS 391

[Close]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 3.6e-122
Identity = 200/348 (57.47%), Postives = 268/348 (77.01%), Query Frame = 0


Query: 4   LQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSGAA 63
           +  WPEP+V VQSLAES + ++P RY+KP S RP+    ++    NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VRE------MAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTY 123
           + +      ++EAC+ WGFF V+NHG+  E+M+  ++ WR+FF  P++ K+ Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGK 183
           EGYGSRLG+EKGA LDWSDY+FL+ +P   ++  KWP+FPP+ +E+I+EYG E++KL G+
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGGEN IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGGEN-IGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVN+GDQIQ+LSN+ YKSV+HRVIVNS+ +RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTS 346
           AFFYNP+SD+ I+P +ELV+   P L+PPMTFD+YRL+IR +G  G S
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKS 362

[Close]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.6e-115
Identity = 194/349 (55.59%), Postives = 259/349 (74.21%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAA--VESEVRRNIPVVDMEKL 60
           M+  Q WPEP+V VQSL+ES +  IP RYVKP SQRP+       +     IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYAN 120
            +         + E+++AC+E GFF V+NHG+S ++M+  K TWR+FF+ P+++K  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPSTYEGYGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVM 180
           SP TYEGYGSRLG+EKGAILDWSDY++L++ P S ++  KWP+ P   +E++E+Y  E++
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCGKVMKGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLC  +MK LSK LGL+E+ L  AFGG+   G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+GDQIQ+LSN+IYKSV+HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 MDRVSLAFFYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRG 341
            +R+SLAFFYNP+ ++ IEP KELVT D PAL+   T+D YR +IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349

[Close]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-66
Identity = 128/310 (41.29%), Postives = 189/310 (60.97%), Query Frame = 0


Query: 24  TIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSGAAVREMAEACKEWGFFHVINHG 83
           T+PG+YV+P SQRP    V S+ R  IPVVD+      A V  + +AC+  GFF V+NHG
Sbjct: 19  TMPGKYVRPESQRPRLDLVVSDAR--IPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHG 78

Query: 84  ISGEMMECVKDTWRKFFDQPLDVK-KQYANSPSTYEGYGSRLGIEKGAILDWSDYFFLNF 143
           I   ++  V +  R+FF  P + K K Y++ P+      +   + K  + +W DY  L+ 
Sbjct: 79  IDAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSFNVRKETVHNWRDYLRLHC 138

Query: 144 MPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSKGLGLEEEYLLKAFGGENG 203
            P+ H+    WP+ PPS KE+I  Y  EV +L  ++ + +S+ LGLE  Y+ +  G +  
Sbjct: 139 YPL-HQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQE- 198

Query: 204 IGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGLQVRKGDDWITIDPIPNA 263
               M  N YP+CP+P+LT GL  H+DP  +TILL D  V GLQV     WI ++P P A
Sbjct: 199 --QHMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGA 258

Query: 264 LIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPRSDLIIEPAKELVTEDRPA 323
           L++N+GDQ+Q LSN  Y+SV HR +VNS+ +R+S+A F  P + + + PAK+L+T+D PA
Sbjct: 259 LVINIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPA 318

Query: 324 LFPPMTFDEY 333
           ++   T+DEY
Sbjct: 319 VYRNYTYDEY 322

[Close]

BLAST of Spg033212.1 vs. ExPASy TrEMBL
Match: A0A6J1G9Z8 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 GN=LOC111452304 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.5e-182
Identity = 308/349 (88.25%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIP RYVKPPSQRPSGAAVE  V RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGY 120
            A V+ MAEACKEWGFFHVINHGI+GEMMEC+KD W +FF QPLD+K+QYANSP+TYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMK 180
           GSRLGIEK AILDWSDYFF+N++P+S RN AKWPAFPP SKELIEEYG E++KLC KVMK
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMK 180

Query: 181 GLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240
           GLS GLGLEEEYLLKAFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH
Sbjct: 181 GLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240

Query: 241 NVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFF 300
           NVPGLQVRKGDDWITIDPIPNALIVNV DQIQVLSN IYKSV HRVIVNSN DRVSLAFF
Sbjct: 241 NVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 300

Query: 301 YNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           YNPRSDLIIEPAK LVTE+RPALFPPMTFDEYRL IR+RGICGTSQQ+Q
Sbjct: 301 YNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQ 348

[Close]

BLAST of Spg033212.1 vs. ExPASy TrEMBL
Match: A0A6J1I649 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN=LOC111470253 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.1e-181
Identity = 308/349 (88.25%), Postives = 323/349 (92.55%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIP RYVKPPSQRPSGAAV+  V RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVKRNVWRNIPVVDMEKLQS 60

Query: 61  GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGY 120
            A V  MAEACKEWGFFHVINHGI+GEMMEC+KD W +FF QPLD+K+QYANSP+TYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMK 180
           GSRLGIEKGAILDWSDYFF+N++P+S RN AKWPAFPP SKELIEEYG E++KLC KVMK
Sbjct: 121 GSRLGIEKGAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMK 180

Query: 181 GLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240
           GLS GLGLEEEYLLKAFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH
Sbjct: 181 GLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDH 240

Query: 241 NVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFF 300
           NVPGLQVRKGDDWITIDPIPNALIVNV DQIQVLSN IYKSV HRVIVNSN DRVSLAFF
Sbjct: 241 NVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 300

Query: 301 YNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           YNPRSDLIIEPAK LVTE+RPALFPPMTFDEYRL IRKRGICGT QQ+Q
Sbjct: 301 YNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTLQQEQ 348

[Close]

BLAST of Spg033212.1 vs. ExPASy TrEMBL
Match: A0A6J1J756 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3661 GN=LOC111481976 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.6e-181
Identity = 306/351 (87.18%), Postives = 332/351 (94.59%), Query Frame = 0


Query: 6   SWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVES-EVRRNIPVVDMEKLQSGAAV 65
           SWPEPVVCVQSLAESGI+TIP RYVKPP QRP GAA+ES EV+RNIP+VDM KL+S AAV
Sbjct: 2   SWPEPVVCVQSLAESGIKTIPVRYVKPPCQRPGGAAMESVEVQRNIPMVDMAKLESAAAV 61

Query: 66  REMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRL 125
           R+MAEAC+EWGFFHVINHGISGE+MECVK++W++FFDQPLD+KKQYANSP TYEGYGSRL
Sbjct: 62  RDMAEACQEWGFFHVINHGISGELMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSK 185
           GIEKGAILDWSDYFFLN+MP S RNPA WPAFPPSSK+LIEEYG+EV+KLCGK+MK LS 
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGSEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPR 305
           LQVRKGD+WITIDPIPNALIVN+GDQIQVLSNAIYKSVKHRVIVNSN DRVSLAFFYNP+
Sbjct: 242 LQVRKGDNWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFLKST 356
           SDLIIEPAKELVTE+RPALFP MTFDEYRL+IRKRGICGTSQ +Q  LKST
Sbjct: 302 SDLIIEPAKELVTEERPALFPRMTFDEYRLHIRKRGICGTSQHEQS-LKST 351

[Close]

BLAST of Spg033212.1 vs. ExPASy TrEMBL
Match: A0A6J1F503 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111442217 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.9e-180
Identity = 306/351 (87.18%), Postives = 329/351 (93.73%), Query Frame = 0


Query: 6   SWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVES-EVRRNIPVVDMEKLQSGAAV 65
           SWPEPVVCVQSLAESGIRTIP RYVKPP QRP GAA+ES EV+ NIPVVDM KL+S AAV
Sbjct: 2   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAAV 61

Query: 66  REMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRL 125
           R+MAEAC+EWGFFHVINHGISGE+MECVK++W++FFDQPLD+KKQYANSP TYEGYGSRL
Sbjct: 62  RDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSK 185
           GIEKGAILDWSDYFFLN+MP S RNPA WPAFPPS+K+LIEEYGNEV+KLCGK+MK LS 
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSTKKLIEEYGNEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGE GIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPR 305
           LQVRKGD WITIDPIPNALIVN+GDQIQVLSNAIYKSVKHRVIVNSN DRVSLAFFYNP+
Sbjct: 242 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFLKST 356
           SDLIIEPAKELV+E+RPALFP MTFDEYRL IRKRGICGTSQ +Q  LKST
Sbjct: 302 SDLIIEPAKELVSEERPALFPRMTFDEYRLDIRKRGICGTSQHEQS-LKST 351

[Close]

BLAST of Spg033212.1 vs. ExPASy TrEMBL
Match: A0A0A0LCS8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731160 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.5e-171
Identity = 282/348 (81.03%), Postives = 318/348 (91.38%), Query Frame = 0


Query: 2   SCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSG 61
           SCLQSWPEPVVCVQSLAESGI  IPGRYVKPPSQRP G  V+    RNIPVVDMEK++SG
Sbjct: 4   SCLQSWPEPVVCVQSLAESGISKIPGRYVKPPSQRPDGGTVKK--MRNIPVVDMEKVESG 63

Query: 62  AAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYG 121
           AAV+EMAEAC+EWGFF +INHGISGEM+ECVK++W++FF+QPLD+K QYAN+P+TYEGYG
Sbjct: 64  AAVKEMAEACREWGFFQIINHGISGEMIECVKESWKEFFNQPLDLKNQYANTPATYEGYG 123

Query: 122 SRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKG 181
           SRLGIEKGAILDWSDYFFLNF+P+S RNP KWPAFP S K++IEEYG+EV+K+C K+MKG
Sbjct: 124 SRLGIEKGAILDWSDYFFLNFLPLSLRNPTKWPAFPSSFKKVIEEYGDEVVKVCAKLMKG 183

Query: 182 LSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 241
           LS GLGLEEEYLL AFGGE GIG  MRANMYPKCPQPDLTLGLS HSDPGGITILL DHN
Sbjct: 184 LSMGLGLEEEYLLNAFGGEKGIGGSMRANMYPKCPQPDLTLGLSSHSDPGGITILLADHN 243

Query: 242 VPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFY 301
           VPGLQV KG+DWIT+DPIPNALIVN+GDQIQVLSN IYKSVKHRV+VN N +RVSLAFFY
Sbjct: 244 VPGLQVLKGNDWITVDPIPNALIVNIGDQIQVLSNGIYKSVKHRVMVNPNKERVSLAFFY 303

Query: 302 NPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQ 350
           NP+ DLIIEP+KEL+T++RP+LFPPMTFDEYRLYIRK+GICGTS+  Q
Sbjct: 304 NPKGDLIIEPSKELLTKERPSLFPPMTFDEYRLYIRKKGICGTSKHQQ 349

[Close]

BLAST of Spg033212.1 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-127
Identity = 207/347 (59.65%), Postives = 266/347 (76.66%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKL-QSGAAVR 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     +S+    IPV+DM  +      +R
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  EMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEGYGSRLG 126
            +  AC+EWGFF ++NHG++  +ME V+  WR+FF+ PL+ K++YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVMKGLSKG 186
           + K A LDWSDYFFLN++P S RNP+KWP+ PP  +ELIE+YG EV KLC ++ + LS+ 
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVN+GDQ+Q+LSN IYKSV+H+VIVNS M+RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTSQQDQQFL 353
           D+ + P +ELVT +RPAL+ P+ FDEYR  IR++G CG +Q D   L
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQVDSLLL 351

[Close]

BLAST of Spg033212.1 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 444.1 bits (1141), Expect = 1.0e-124
Identity = 200/346 (57.80%), Postives = 271/346 (78.32%), Query Frame = 0


Query: 5   QSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESE---VRRNIPVVDMEKLQSG 64
           + WPEP+V VQSLAES + ++P RY+KPPSQRP    ++ +      NIP++D++ L SG
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AA--VREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEG 124
                + ++EAC+EWGFF VINHG+  E+M+  ++TW+ FF+ P++ K+ Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVM 184
           YGSRLG+EKGAILDW+DY++L+F+P++ ++  KWP+ P + +E+ +EYG E++KL G++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVN+GDQIQ+LSN+ YKSV+HRVIVNS  +RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTS 346
           FYNP+SD+ I+P ++LVT   P L+PPMTFD+YRL+IR +G  G S
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKS 391

[Close]

BLAST of Spg033212.1 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 4.0e-124
Identity = 198/341 (58.06%), Postives = 269/341 (78.89%), Query Frame = 0


Query: 5   QSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESE---VRRNIPVVDMEKLQSG 64
           + WPEP+V VQSLAES + ++P RY+KPPSQRP    ++ +      NIP++D++ L SG
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AA--VREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTYEG 124
                + ++EAC+EWGFF VINHG+  E+M+  ++TW+ FF+ P++ K+ Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGKVM 184
           YGSRLG+EKGAILDW+DY++L+F+P++ ++  KWP+ P + +E+ +EYG E++KL G++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVN+GDQIQ+LSN+ YKSV+HRVIVNS  +RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRG 341
           FYNP+SD+ I+P ++LVT   P L+PPMTFD+YRL+IR +G
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQG 386

[Close]

BLAST of Spg033212.1 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 2.6e-123
Identity = 200/348 (57.47%), Postives = 268/348 (77.01%), Query Frame = 0


Query: 4   LQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAAVESEVRRNIPVVDMEKLQSGAA 63
           +  WPEP+V VQSLAES + ++P RY+KP S RP+    ++    NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VRE------MAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYANSPSTY 123
           + +      ++EAC+ WGFF V+NHG+  E+M+  ++ WR+FF  P++ K+ Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVMKLCGK 183
           EGYGSRLG+EKGA LDWSDY+FL+ +P   ++  KWP+FPP+ +E+I+EYG E++KL G+
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGGEN IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGGEN-IGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSNMDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVN+GDQIQ+LSN+ YKSV+HRVIVNS+ +RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRGICGTS 346
           AFFYNP+SD+ I+P +ELV+   P L+PPMTFD+YRL+IR +G  G S
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKS 362

[Close]

BLAST of Spg033212.1 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 415.6 bits (1067), Expect = 4.0e-116
Identity = 194/349 (55.59%), Postives = 259/349 (74.21%), Query Frame = 0


Query: 1   MSCLQSWPEPVVCVQSLAESGIRTIPGRYVKPPSQRPSGAA--VESEVRRNIPVVDMEKL 60
           M+  Q WPEP+V VQSL+ES +  IP RYVKP SQRP+       +     IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------GAAVREMAEACKEWGFFHVINHGISGEMMECVKDTWRKFFDQPLDVKKQYAN 120
            +         + E+++AC+E GFF V+NHG+S ++M+  K TWR+FF+ P+++K  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPSTYEGYGSRLGIEKGAILDWSDYFFLNFMPISHRNPAKWPAFPPSSKELIEEYGNEVM 180
           SP TYEGYGSRLG+EKGAILDWSDY++L++ P S ++  KWP+ P   +E++E+Y  E++
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCGKVMKGLSKGLGLEEEYLLKAFGGENGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLC  +MK LSK LGL+E+ L  AFGG+   G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNVGDQIQVLSNAIYKSVKHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+GDQIQ+LSN+IYKSV+HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 MDRVSLAFFYNPRSDLIIEPAKELVTEDRPALFPPMTFDEYRLYIRKRG 341
            +R+SLAFFYNP+ ++ IEP KELVT D PAL+   T+D YR +IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349

The following BLAST results are available for this feature:

BLAST of Spg033212.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903719.1	3.5e-183	88.70	probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida]	[more]
KAG6596343.1	5.1e-182	88.54	putative 2-oxoglutarate-dependent dioxygenase ANS, partial [Cucurbita argyrosper...	[more]
XP_022948721.1	1.1e-181	88.25	probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita moschata]	[more]
XP_023539287.1	2.5e-181	87.97	probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita pepo subsp. pepo]	[more]
XP_023528714.1	3.3e-181	87.75	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. p...	[more]

BLAST of Spg033212.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80449	3.2e-126	59.65	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q9SRM3	1.5e-123	57.80	Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1	[more]
Q9FFF6	3.6e-122	57.47	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
Q9LY48	5.6e-115	55.59	Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1	[more]
Q8W2X5	2.1e-66	41.29	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]

BLAST of Spg033212.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9Z8	5.5e-182	88.25	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1I649	2.1e-181	88.25	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1J756	3.6e-181	87.18	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3...	[more]
A0A6J1F503	3.9e-180	87.18	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A0A0LCS8	1.5e-171	81.03	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]

BLAST of Spg033212.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38240.1	2.2e-127	59.65	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.1	1.0e-124	57.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.2	4.0e-124	58.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	2.6e-123	57.47	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G55970.1	4.0e-116	55.59	jasmonate-regulated gene 21	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 67..84 score: 43.43 coord: 291..309 score: 38.76
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 3..340
None	No IPR available	PANTHER	PTHR47991:SF14	LEUCOANTHOCYANIDIN DIOXYGENASE-LIKE	coord: 3..340
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..338
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 50..155 e-value: 1.7E-25 score: 90.1
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 205..303 e-value: 6.2E-27 score: 94.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..339 e-value: 2.3E-109 score: 367.7
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 202..303 score: 14.181684

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg033212	Spg033212	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033212.1-five_prime_utr	Spg033212.1-five_prime_utr-scaffold5:1060591..1060640	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033212.1-cds	Spg033212.1-cds-scaffold5:1060641..1061122	CDS
Spg033212.1-cds	Spg033212.1-cds-scaffold5:1062829..1063162	CDS
Spg033212.1-cds	Spg033212.1-cds-scaffold5:1063556..1063807	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg033212.1-three_prime_utr	Spg033212.1-three_prime_utr-scaffold5:1063808..1064201	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg033212.1	Spg033212.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900366	negative regulation of defense response to insect
biological_process	GO:1900150	regulation of defense response to fungus
biological_process	GO:2000022	regulation of jasmonic acid mediated signaling pathway
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0120091	jasmonic acid hydrolase
molecular_function	GO:0046872	metal ion binding