Spg030671.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg030671.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	HNHc domain-containing protein
Location	scaffold11: 25924469 .. 25929769 (-)
Sequence length	819
RNA-Seq Expression	Spg030671.1
Synteny	Spg030671.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGGACTTTTCCGTGAGCATGTAATGCTCTTTTCTTCTTCCCCACAACTACTGGGCTTTGATTTCTTCATCCGATGAGCAATCTCGATTTTGATCTCCATTGACTGCTAGCAGGAGCCAATTTCAGTACAGAAATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGAATCCCTTTATCTGGTGCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTCCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGAGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTGACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGTAAATTCACCTTCATACCCGCTTTGGTGTTTTTAATCGATGAGCTCTTTTGAGTGATGTGCGACTCTGTTATCTGTTAGAGTGTTAATAGGGAAATAATTGGAATGTTGTTAGGACATTAATCGTGGCATAGTTGTACTTAGTTAGGAAGTTAGTTAGAGTGCATGGCTATAAATAGCTGGAGTTGGGAGTTTAAGAATTAAGCATAATTTTAGGAATTGGTCTAATATAGGGAAAATTATTATAGGACTTGGGAGAGAGAACCGTATCCGCTATTGTTTTTGTAGTGTCTTCGTTTTGTCATTTTCTTATAACTTGGATCCTAACATTATCAATTTATTTCCTATTGTTGTCTTCGATGAAAAATCTTAACGCTTTCCGAGTACCTTTTAACTTGTCCCTTTTTTCTTTTGGGTGAAATTATGGGTAGAGTTCGACTTCTAGACATATTTATTATCAATATTGCATTTTCGGTTAGCTGTTTACTTGGCCTTACCGTGTTTAAACGAATAAAAAATTGCCACATTAGTTAATGTTGAAGCAAATGCTAGCTTGGAGTAGGTCAATGATTTAACAAAATTCTGATATTGGAGCTTAAAGTGGGGCCAGGCATTAACACAAGAGGAAACATGAAGCCAGCAAGTATATCAAATTGATTATTCTTGATTCTGTCCGAACAATCAGGAGCTAATATATCATGTATTTGAAGTATTGCAGATCATTTGTCCTTCATGTAGGTATAGAATTTCAATTACTATTATGAAATACTTTTACGCGTTTGAGTTCGATTAGAAGTTTCTAGGAGGCACAATAAGCAGAGCTTGTGTTTTTCCTATTTATAGCCGAGGTTTAAAAGTAGGCAGCCCAGAAATGGATATATATCCAGCTCAACGTGTATACAGTTGAAAGCAATATGAAGGAAAAGAAGTTGACTAAAAGAATAAAATATTGAGTGTGTTGTCTATCCAATACATCTGCCCGATCAGGACTGATCTTGCATGTCGAGCTTTGTCTATGAACGACCTCTTGGCAAAGCCTTTTTTTTTCTTAAAAAAGATCAGCCATCTGAGCTTTCACAAGAGAATGCAGTGAATCTCGACTTCTATATTCTTGAAATGCTCTCGTATAAAGTGCCTGTATTGCCCTTTATATTTGTGTCCTGGAGTAAAGAACAAGATTTGATGCTAATGATGCAACCTCCATGTTATTGAAGATATCAATAGCATGAGTAAGGTTGCCATCCTAATCATTTTCTTAAAAATGTATGGCTATGGAACATGAGGAACATGATTCCAATGGAACAACCTTTGATAGTTTGATTCAATGTTGAGGCGTTGGATAAGGGTGTGGATAAGGGCTTTTTAAGCCTTTTCATTACCTATAATCAGTTTTCAGGGCCTCAATGTTAATTTCTGATCTTTGGATTGTTAAGTTCACTGATCATATGCTTGTTGGCCAACAGTCCAAATACCATACTGAACTTGATACGGTCCCCTGGATGCAAAATAGCTGCCAACCAGAACGATCTTGCATAGGTGTAAAGCAATATGAAGAAAGAAAATAAGTTAGTTAAAAGAATATGGGTTGCAGTTGCACCAACCCCCACCCGCAAGCCAGATTTATCTTGTGCACTGAGGTTGTCAAACGTAAACAAAGAAAGTCACCTCTTAGCTTTACTGAAAGTATGGAGCCTTTGGTCTTTTCAGGGAACATAGCAAATTTTACCTTTCCATTTATCCTTCCAGTAGCATAAAATTTTAAATCCAATTATTTGATATGACTGCCATTTTCACTTTAAGAGCCAATTCGTTCTAGCACCATACTTCTGGATTCTTGCAAATGTTGATACAATGTAAACAAGGGATGACTGAAGTGGCTTCTTTAGATAATGCCTCATTCTCAACATTTCAGCTATTTTAGATAAACATGGAGCCACCAAGATATTTTGTGTCCATTCCTGCATGTGGTACTACGCACTGAAGAGAACGAACAAACACAAACAAACTCCACTTTTTCCAGTACTTTTTGCTTTAAAATTTTTCGTTCATTTTCTACTATTTAGTCAAACAAATACTTGACTTGATTTCTGTATGTGGAATGGATGACCTACACTTTTATGTGTATACCTGTTTCATATTGCCTTTATGTCTCCATATTATTTGTATGACTGGAAAGGTTTGCAATTTATCTTTTGCTATTGATTTCCTTGCTTCAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGTTTGACTAATCTCTTTTATGTATTATGTATAAAAATAAAGATAATATATAGTTCTTAGTGTAACAATTAATTGGTTTCATGTTTCTTATTGAAAATATGTCTAGAAGATGAGGAAGTTATCATCTTTTGGAAAATTTTGAGTTCATAGTCAGGGGAAATATAGTAAGTCTGTCCAATTAGCGAGAGTGAAAAGCCAAATGGCAGTGGCTAGATGCTTTGGTATTTAAATTGTCACTCTCTGCAATTTATATATCTTTGCAGAAAGTCTGTCAATTTTAAATCTTGATTACAGATTTCAAAAGGTTTAAATAACGATTTGGTCTTATCAATGCATGTTTCGATATGCCTTTATCTCAAACCTTTTTGGCTTTTCCAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTATGCCTAAACCATGTATCTAGTTCATTCATGGTTTTTTTTCGTAGGATTGTTACATGTTATCGGTCTTCATTTCCATAAGAAGTTCTAGGCTGAATTCATCTTTTTGACTTGAATATTTCCTTGTCAAAGGCCCTTGAACTAACTATATCGTCTCTCTTTTCAGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGGATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTAAGTTGATAAATATTCACAATAGTTTTATTCAATTTATCATTTAAAAATTAAAATTGATATGGAAGAAGTTATTTTGAGATGGTTTGCTAATTTTATGATGGGGAGGCATGTTTTGATTATGTAATCATTTTAATTGTAGCTTCACTAAACATGCTTTGTTATTTTAATTGTATTGCTATTTTTATGACGGCAGATGGCACACTCCACTTTTTTTTCTTTTTTTTTTTTTTGTTTTGCTCGTTAATATTAGTGCCCATTGGGTTTGCATTGGCTGTGTCTCAAATCTCGTGCCCGAAGCGTCCATCCCAATGATAAAGGGTAACGAGAAATCAGGTAAGGCCAACATATGCAGTGCTATCATAGCCTTAATAGCCTCTGAATTGCATGTGAGCTATCCCTTTTGAGCCTTTTGTAAGGGGAGAAGAGGTATGCATTATAAGTTGTCATGAAGCAACCTTATTACCCTGTCATCCCCAGATAAGCGAATAATTCTTTCAAAGATTTCAGAGATGGCCACTGTATGGCCTGCACGTTACTGGTCCTTTACACCTTTGGATGAGATCCAAACGAAGCCTTATTATCTAAAATGGCTTTGGAGGTTCTTGACTGAGTTTGATTAGTTTTGGTAGATTTAGGTTTTTTAAATTCTTAACACTACTTTCAATAGGAAGGTAACTAGACTGAATATTGAAATGAGGGATGATATTGCAAGGATCTGAATGGAAAATCTTTTGCTCCACTTCTTTTTAGTTCTTGTTATTTTCTGGCAAAGGGTGAATACTAATTTCATCCAAGACAACTTTTATGTGCTGTCTTTTTTTTTCTTTATTTTGTTTGTTGCCTCAAATGGATCTTTTATCAACAGGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTGAGTTAACCAGTAGAAATTAATATTATATTACAAATGCTCAACTGATCCTGATTCCAAAACAGAGAGATGTCCAGAGATTTTCCTGGTAATAAAATAGAACACAAAATCCTACCTAATAACAGGAGTTTAAAGGCCAAATTGGCAAAGCATCTTCAGTTTCATTTGAACAATTCAGCTGCTACATTGTTAATTCTGAGACCATATAAATATGTCGAGTTCCAAAAACTGTTCTGTGGTTATAGCTATTGGGGATTGGATGTTTGGAAAATGGGACTTCTAAAAAGGAGTTGCTATGATGCTTACCAACAACTCTTCATTTACGAAATTGGTTCACTGTTTTCTGTTTTCTCTAATCCCTTATGTTGTAATAGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGTTATATTATGTTTTGTTTATAGTGAGAAATTGAATTAAATATTATGTTTATGACAGAAAGAAACTTCTGATTTTCTTTTCTTCCGTGTGTGTGTGGGTGAAAATCCGTCTGCTATGCTTCATTATTCTAATGTTTGATCAGGTCTCACTGCATCTAGTTCAGACTTATTGATTTTTACCTCGAATTTTTCAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGATGTGTATTTATAGTGGCACTTGTAAATTCTTCTTTGCACATATTGCACAAATTGCACATATCTACTACACTTGGTTCTTCTAAAAGACTCTTGTGGCACCTTGAAAGTAATTTATTCTATCATTAGTTCCCATAAGCATTTGGCATTTCTCTTTA

mRNA sequence

ATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGAATCCCTTTATCTGGTGCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTCCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGAGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTGACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGGATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

Coding sequence (CDS)

ATGGCCCAATTCACCGCACACAGTCGGGTTAAGTTGCTGCTCAACGGAGACGGAGTGCCATTTGAAGATCGATTGAGTTACAAGCTCAGATCAGTGCGAACCCTTAAGCGTAGAATCCCTTTATCTGGTGCCTCCTCTGCACTTTCCCCTTCTGCATCCTCTGCTCCAGCTTTGAGGAAATCCGCTCTGCGTGTTAGTGTGAGAGGTGAGAGCGTTAGCGGTGACGACGCCATTGTTGATCTTGACTACGATTACGAGTTTGAGAGTGACGATTTGGCTTGCTTCAGAGGTCTCGTCTTGGATATTTCCTACAGGCCAGTCAACGTTGTTTGTTGGAAGCGTGCAATTTGTTTGGAGTTCATGGAGAAGGCTGATGTATTGGAATACTACGACCAGACTGTGAGTTCTCCAAGTGGATCCTTCTATATACCAGCAGTCTTAAGGGTTCCCCATTTATTGCAAGTTGTAAAGAGAAGAAGGATCAAGAACTCTTTGAGTCGTAAAAACATACTTTATCGGGACAATTACACTTGTCAGTATTGTTCATCACATGAGAGTTTGACCATTGACCATGTTTTGCCCATATGCCGGGGTGGAGAATGGACATGGGAAAATCTGGTTGCTGCCTGTGTAAAATGCAATTCAAAGAAAGGTCAGAAAACTGTAGAAGAAGCAAATATGAAGCTGAAAAAGACTCCCAAGGCCCCAAAAGATTATGATATACTTGCCATTCCTCTAACGAGTACCGCAATAAAGATGTTGAAACTGAGAAAGGGGACCCCTGAAGAATGGCGTCAATATCTGTCAAGTGAGCAATGA

Protein sequence

MAQFTAHSRVKLLLNGDGVPFEDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAPALRKSALRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ

Homology

[Close]

BLAST of Spg030671.1 vs. NCBI nr
Match: XP_022932702.1 (uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022972084.1 uncharacterized protein LOC111470725 [Cucurbita maxima] >XP_023539770.1 uncharacterized protein LOC111800353 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 3.1e-139
Identity = 257/277 (92.78%), Postives = 263/277 (94.95%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASS-ALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS  LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  PALRKSALRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
            ALRKSA RV VRGESVSGDDAI+D+DYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

[Close]

BLAST of Spg030671.1 vs. NCBI nr
Match: KAG6597719.1 (hypothetical protein SDJN03_10899, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029166.1 hypothetical protein SDJN02_10351, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 256/277 (92.42%), Postives = 262/277 (94.58%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASS-ALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR   KLRS+RTLKRR PLSGASS  LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRQKLRSLRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  PALRKSALRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
            ALRKSA RV VRGESVSGDDAI+D+DYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

[Close]

BLAST of Spg030671.1 vs. NCBI nr
Match: XP_038905692.1 (uncharacterized protein LOC120091663 [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 8.5e-137
Identity = 256/281 (91.10%), Postives = 260/281 (92.53%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  YKLR VRTLKRRIPLSG     SPS SSA 
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRFRYKLRLVRTLKRRIPLSGP----SPSTSSAS 60

Query: 61  ALRKSA-----LRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVC 120
           ALRKSA     +RV VRGESVSGDDAIVDLDYDYEFE+DDLACFRGLVLDISYRPVNVVC
Sbjct: 61  ALRKSAQHAAEVRVGVRGESVSGDDAIVDLDYDYEFETDDLACFRGLVLDISYRPVNVVC 120

Query: 121 WKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180
           WKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL
Sbjct: 121 WKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180

Query: 181 YRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK 240
           YRDNYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACV+CNSKKGQKTVEEANMKLKK
Sbjct: 181 YRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVQCNSKKGQKTVEEANMKLKK 240

Query: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

[Close]

BLAST of Spg030671.1 vs. NCBI nr
Match: XP_022152374.1 (uncharacterized protein LOC111020122 [Momordica charantia])

HSP 1 Score: 475.3 bits (1222), Expect = 3.5e-130
Identity = 247/281 (87.90%), Postives = 255/281 (90.75%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFT  +RVKLLLNGDGVPF    +DRL YKLRSV    RRIPLS AS+ +SPSASSA 
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV----RRIPLSAASTGISPSASSAS 60

Query: 61  ALRKSA-----LRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVC 120
           ALRKSA     +RV VR ESVS D AIV LD DYEFESDDLACFRGLVLDISYRPVNVVC
Sbjct: 61  ALRKSAQHVAEMRVGVRDESVSDDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVVC 120

Query: 121 WKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180
           WKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL
Sbjct: 121 WKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180

Query: 181 YRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK 240
           YRDNYTCQYCSSH+SLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK
Sbjct: 181 YRDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK 240

Query: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

[Close]

BLAST of Spg030671.1 vs. NCBI nr
Match: XP_008465340.1 (PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH endonuclease [Cucumis melo var. makuwa] >TYK29998.1 HNH endonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 465.3 bits (1196), Expect = 3.6e-127
Identity = 245/282 (86.88%), Postives = 250/282 (88.65%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SS    S SS  
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSS----STSSTS 60

Query: 61  ALRKSA-----LRVSVRGESVS-GDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVV 120
           ALRKS      +RV VR ESV+ GDDAIV  DYDYEFESDDLACFRGLVLDISYRPVNVV
Sbjct: 61  ALRKSTQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

[Close]

BLAST of Spg030671.1 vs. ExPASy TrEMBL
Match: A0A6J1F2H9 (uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC111439170 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.5e-139
Identity = 257/277 (92.78%), Postives = 263/277 (94.95%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASS-ALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS  LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  PALRKSALRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
            ALRKSA RV VRGESVSGDDAI+D+DYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

[Close]

BLAST of Spg030671.1 vs. ExPASy TrEMBL
Match: A0A6J1IAG4 (uncharacterized protein LOC111470725 OS=Cucurbita maxima OX=3661 GN=LOC111470725 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.5e-139
Identity = 257/277 (92.78%), Postives = 263/277 (94.95%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASS-ALSPSASSA 60
           MAQFTAHSRVKLLLNGDGVPF    +DR  +KLRSVRTLKRR PLSGASS  LSPS+SSA
Sbjct: 1   MAQFTAHSRVKLLLNGDGVPFGSEPKDRSRHKLRSVRTLKRRTPLSGASSTGLSPSSSSA 60

Query: 61  PALRKSALRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120
            ALRKSA RV VRGESVSGDDAI+D+DYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA
Sbjct: 61  SALRKSAQRVGVRGESVSGDDAILDIDYDYEFESDDLACFRGLVLDISYRPVNVVCWKRA 120

Query: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNILYRDN 180
           ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIK SLSRKNILYRDN
Sbjct: 121 ICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKQSLSRKNILYRDN 180

Query: 181 YTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240
           YTCQYCSSHESLTIDHVLP+ RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA
Sbjct: 181 YTCQYCSSHESLTIDHVLPVSRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKKTPKA 240

Query: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 PKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 277

[Close]

BLAST of Spg030671.1 vs. ExPASy TrEMBL
Match: A0A6J1DFU6 (uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020122 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 1.7e-130
Identity = 247/281 (87.90%), Postives = 255/281 (90.75%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPF----EDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFT  +RVKLLLNGDGVPF    +DRL YKLRSV    RRIPLS AS+ +SPSASSA 
Sbjct: 1   MAQFTTLNRVKLLLNGDGVPFGSESKDRLRYKLRSV----RRIPLSAASTGISPSASSAS 60

Query: 61  ALRKSA-----LRVSVRGESVSGDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVVC 120
           ALRKSA     +RV VR ESVS D AIV LD DYEFESDDLACFRGLVLDISYRPVNVVC
Sbjct: 61  ALRKSAQHVAEMRVGVRDESVSDDGAIVGLDCDYEFESDDLACFRGLVLDISYRPVNVVC 120

Query: 121 WKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180
           WKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL
Sbjct: 121 WKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNIL 180

Query: 181 YRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK 240
           YRDNYTCQYCSSH+SLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK
Sbjct: 181 YRDNYTCQYCSSHDSLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLKK 240

Query: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS+EQ
Sbjct: 241 TPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSNEQ 277

[Close]

BLAST of Spg030671.1 vs. ExPASy TrEMBL
Match: A0A5D3E2H2 (HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00280 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.7e-127
Identity = 245/282 (86.88%), Postives = 250/282 (88.65%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SS    S SS  
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSS----STSSTS 60

Query: 61  ALRKSA-----LRVSVRGESVS-GDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVV 120
           ALRKS      +RV VR ESV+ GDDAIV  DYDYEFESDDLACFRGLVLDISYRPVNVV
Sbjct: 61  ALRKSTQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

[Close]

BLAST of Spg030671.1 vs. ExPASy TrEMBL
Match: A0A1S3CNK0 (uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.7e-127
Identity = 245/282 (86.88%), Postives = 250/282 (88.65%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVP----FEDRLSYKLRSVRTLKRRIPLSGASSALSPSASSAP 60
           MAQFTAHSRVKLLLNGDG+P     +DR  YKLRSVR   RR PLS  SS    S SS  
Sbjct: 1   MAQFTAHSRVKLLLNGDGLPCGSESKDRFRYKLRSVRA--RRFPLSAPSS----STSSTS 60

Query: 61  ALRKSA-----LRVSVRGESVS-GDDAIVDLDYDYEFESDDLACFRGLVLDISYRPVNVV 120
           ALRKS      +RV VR ESV+ GDDAIV  DYDYEFESDDLACFRGLVLDISYRPVNVV
Sbjct: 61  ALRKSTQHAAEVRVGVRDESVTGGDDAIVGFDYDYEFESDDLACFRGLVLDISYRPVNVV 120

Query: 121 CWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180
           CWKRAICLEFMEKADVLEYYDQTV+SPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI
Sbjct: 121 CWKRAICLEFMEKADVLEYYDQTVNSPSGSFYIPAVLRVPHLLQVVKRRRIKNSLSRKNI 180

Query: 181 LYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240
           LYRDNYTCQYCSSHESLTIDHVLPI RGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK
Sbjct: 181 LYRDNYTCQYCSSHESLTIDHVLPISRGGEWTWENLVAACVKCNSKKGQKTVEEANMKLK 240

Query: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 273
           KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ
Sbjct: 241 KTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLSSEQ 276

[Close]

BLAST of Spg030671.1 vs. TAIR 10
Match: AT2G23840.1 (HNH endonuclease )

HSP 1 Score: 329.7 bits (844), Expect = 2.2e-90
Identity = 175/286 (61.19%), Postives = 214/286 (74.83%), Query Frame = 0


Query: 1   MAQFTAHSRVKLLLNGDGVPFEDRLSYKLRSVRTLKRRIPLSGASSALSP---------- 60
           MA F+A  R+KLL + DG      LS+ + S    ++ + ++G  S L P          
Sbjct: 1   MAHFSA--RLKLLSSNDG------LSFGVDSRDQFRQSLYMAGGGSPLVPIRMSRLKFCA 60

Query: 61  ---SASSAPALRKSALRVSVRGESVSGDDAIVDLDYDYE---FESDD-LACFRGLVLDIS 120
              S+ S P  RK    +    +++  D+   + D+D +    E+DD L+CFRGLVLDIS
Sbjct: 61  RIFSSYSFPYKRKKIRDLDNTEKNLDIDEDNDEWDFDGDDDGLETDDHLSCFRGLVLDIS 120

Query: 121 YRPVNVVCWKRAICLEFMEKADVLEYYDQTVSSPSGSFYIPAVLRVPHLLQVVKRRRIKN 180
           YRPVNVVCWKRAICLE+M+KADVLEYYDQTVSSP+GSFYIPAVLRVPHLLQVVKRRR+KN
Sbjct: 121 YRPVNVVCWKRAICLEYMDKADVLEYYDQTVSSPTGSFYIPAVLRVPHLLQVVKRRRVKN 180

Query: 181 SLSRKNILYRDNYTCQYCSSHESLTIDHVLPICRGGEWTWENLVAACVKCNSKKGQKTVE 240
           SLSRKNIL RD+YTCQYCSS E+LTIDHV+P+ RGGEWTW+NLVAAC +CNS+KGQKT +
Sbjct: 181 SLSRKNILLRDDYTCQYCSSRENLTIDHVMPVSRGGEWTWQNLVAACSRCNSRKGQKTAD 240

Query: 241 EANMKLKKTPKAPKDYDILAIPLTSTAIKMLKLRKGTPEEWRQYLS 270
           EA+MKL K PK PKDYDI+AIPLT+ AI+ML+  KG PEEWRQYL+
Sbjct: 241 EAHMKLLKVPKEPKDYDIVAIPLTNAAIRMLRSNKGMPEEWRQYLA 278

The following BLAST results are available for this feature:

BLAST of Spg030671.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932702.1	3.1e-139	92.78	uncharacterized protein LOC111439170 [Cucurbita moschata] >XP_022932703.1 unchar...	[more]
KAG6597719.1	1.6e-138	92.42	hypothetical protein SDJN03_10899, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038905692.1	8.5e-137	91.10	uncharacterized protein LOC120091663 [Benincasa hispida]	[more]
XP_022152374.1	3.5e-130	87.90	uncharacterized protein LOC111020122 [Momordica charantia]	[more]
XP_008465340.1	3.6e-127	86.88	PREDICTED: uncharacterized protein LOC103502982 [Cucumis melo] >KAA0051262.1 HNH...	[more]

BLAST of Spg030671.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg030671.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2H9	1.5e-139	92.78	uncharacterized protein LOC111439170 OS=Cucurbita moschata OX=3662 GN=LOC1114391...	[more]
A0A6J1IAG4	1.5e-139	92.78	uncharacterized protein LOC111470725 OS=Cucurbita maxima OX=3661 GN=LOC111470725...	[more]
A0A6J1DFU6	1.7e-130	87.90	uncharacterized protein LOC111020122 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A5D3E2H2	1.7e-127	86.88	HNH endonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G002...	[more]
A0A1S3CNK0	1.7e-127	86.88	uncharacterized protein LOC103502982 OS=Cucumis melo OX=3656 GN=LOC103502982 PE=...	[more]

BLAST of Spg030671.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23840.1	2.2e-90	61.19	HNH endonuclease	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003615	HNH nuclease	SMART	SM00507	HNH_5	coord: 165..215 e-value: 6.7E-14 score: 62.2
IPR003615	HNH nuclease	CDD	cd00085	HNHc	coord: 167..218 e-value: 3.98169E-13 score: 60.9483
None	No IPR available	GENE3D	1.10.30.50		coord: 153..242 e-value: 5.6E-23 score: 82.6
None	No IPR available	PANTHER	PTHR33877	SLL1193 PROTEIN	coord: 68..270
None	No IPR available	PANTHER	PTHR33877:SF2	SLL1193 PROTEIN	coord: 68..270
IPR029471	HNH endonuclease 5	PFAM	PF14279	HNH_5	coord: 178..229 e-value: 3.2E-14 score: 52.5

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg030671	Spg030671	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg030671.1-three_prime_utr	Spg030671.1-three_prime_utr-scaffold11:25924469..25924623	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25924624..25924740	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25924934..25925017	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25925392..25925473	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25926342..25926433	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25926600..25926674	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25927052..25927106	CDS
Spg030671.1-cds	Spg030671.1-cds-scaffold11:25929322..25929635	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg030671.1-five_prime_utr	Spg030671.1-five_prime_utr-scaffold11:25929636..25929769	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg030671.1	Spg030671.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity