Spg028805.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg028805.1 vs. NCBI nr
Match: XP_008465212.1 (PREDICTED: omega-3 fatty acid desaturase, chloroplastic [Cucumis melo] >KAA0052881.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa] >TYK14479.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 787.3 bits (2032), Expect = 6.0e-224
Identity = 380/445 (85.39%), Postives = 389/445 (87.42%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNGFAS+T MVSEIR PLL SKA  LRSSLG+STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVS PV +ASVDEDNERERVD VNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK++RSLDKATRTL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPYLMFVMWLD VTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPVFGKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. NCBI nr
Match: XP_038892287.1 (omega-3 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 785.4 bits (2027), Expect = 2.3e-223
Identity = 379/445 (85.17%), Postives = 390/445 (87.64%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNG AS+T MVSEIR PLLASKA  LRSSLGLST+GKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGIASKTFMVSEIRQPLLASKAHVLRSSLGLSTRGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVSAPVRVAS+DEDNE+ERVDG+NGVEDEG FDPAA PPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSAPVRVASIDEDNEKERVDGINGVEDEGDFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PW+SMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWKSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+RSLDKATRTL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPYLMFVMWLD VTYLHHHGH+DKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHEDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPV GKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. NCBI nr
Match: NP_001274404.1 (omega-3 fatty acid desaturase [Cucumis sativus] >ACE80931.1 omega-3 fatty acid desaturase [Cucumis sativus] >KGN50667.1 hypothetical protein Csa_005845 [Cucumis sativus])

HSP 1 Score: 779.6 bits (2012), Expect = 1.3e-221
Identity = 376/445 (84.49%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYP PSRNGFAS+T MVSEIR PLLASKA  LRSSLGLSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVS PV VASVDEDNERERV+ VNGVEDEG FDPAAPPPFRLADIRAAIPKHCWV+D
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVEDEGSFDPAAPPPFRLADIRAAIPKHCWVRD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P LNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+RSLDKATRTL
Sbjct: 181 PNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RF LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQI+KLYGIPY+MFVMWLD VTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQIIKLYGIPYMMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKP+FGKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVVHHLFPQIPHYHLIEATEATKPIFGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. NCBI nr
Match: XP_022995701.1 (omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 760.4 bits (1962), Expect = 7.9e-216
Identity = 372/445 (83.60%), Postives = 382/445 (85.84%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYP+PSRNG ASRT MVSEIR   + SK   LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPRPSRNGLASRTYMVSEIRFSSVPSKPNFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVSAP RVASVDEDNE+++VD   GVE+EGGFDPAA PPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSAPARVASVDEDNEKDKVD---GVENEGGFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFR+LDKATRTL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRTLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPY+MFVMWLDVVTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPV GKYYR+PEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. NCBI nr
Match: KAG7030666.1 (Omega-3 fatty acid desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 760.0 bits (1961), Expect = 1.0e-215
Identity = 369/445 (82.92%), Postives = 383/445 (86.07%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVK LPRIYP+PSRNG ASRTCMVSEIRS  + SK   LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVSAPVRVASVDEDNE+++VD   GVE+EGGFDPAA PPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVD---GVENEGGFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+++LDKAT+TL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATKTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYP+YLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
            VMGPLQILKLYGIPY+MFVMWLDVVTYLHHHGHDDKLPWYRGE                
Sbjct: 301 AVMGPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPV GKYYR+PEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. ExPASy Swiss-Prot
Match: P48619 (Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis OX=3988 GN=FAD7A-1 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 3.5e-174
Identity = 305/452 (67.48%), Postives = 344/452 (76.11%), Query Frame = 0

Query: 2   ARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLS----TK 61
           A WVLSECG++PLPRIY +P R GF S+T  + ++R  L  SK+  L SS  +S    +K
Sbjct: 3   AGWVLSECGLRPLPRIYSRP-RIGFTSKTTNLLKLRE-LPDSKSYNLCSSFKVSSWSNSK 62

Query: 62  GKSWALNVSAPVRVASV--DEDNERERVDGVNGVEDEGG--FDPAAPPPFRLADIRAAIP 121
             +WALNV+ PV V++V  ++D ERE  +G+  V++  G  FD  APPPF LADIRAAIP
Sbjct: 63  QSNWALNVAVPVNVSTVSGEDDREREEFNGIVNVDEGKGEFFDAGAPPPFTLADIRAAIP 122

Query: 122 KHCWVKDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCG 181
           KHCWVK+PWRSMSYV+RDVVVVFGLAAVAAYFNNWV WPLYWF QGTMFWALFVLGHDCG
Sbjct: 123 KHCWVKNPWRSMSYVLRDVVVVFGLAAVAAYFNNWVAWPLYWFCQGTMFWALFVLGHDCG 182

Query: 182 HGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSL 241
           HGSFSN+PKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIF+SL
Sbjct: 183 HGSFSNNPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFKSL 242

Query: 242 DKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAML 301
           D  T+TLRF+LPFPMLAYP YLWSRSPGKKGSHF P+S LFVP ER DIITSTACWTAM 
Sbjct: 243 DNVTKTLRFSLPFPMLAYPFYLWSRSPGKKGSHFHPDSGLFVPKERKDIITSTACWTAMA 302

Query: 302 ALLGCLSVVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE--------- 361
           ALL  L+  MGP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+         
Sbjct: 303 ALLVYLNFSMGPVQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKAWSYLRGGL 362

Query: 362 -------------------------------------TEATKPVFGKYYREPEKSWPLPF 400
                                                TEA KPV GKYYREP+KS PLP 
Sbjct: 363 TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVMGKYYREPKKSGPLPL 422

BLAST of Spg028805.1 vs. ExPASy Swiss-Prot
Match: P48620 (Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum OX=4182 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.3e-172
Identity = 305/460 (66.30%), Postives = 342/460 (74.35%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLR---------SS 60
           MA WVLSECG++PLPR+YP+P R G             PLL S    LR         SS
Sbjct: 1   MASWVLSECGLRPLPRVYPKP-RTG------------HPLLNSNPTKLRFSRTDLGNGSS 60

Query: 61  LGLST---KGKSWALNVSAPVRVASVDEDNER---ERVDGVNGVEDEGGFDPAAPPPFRL 120
             LS+   + K+WAL VSAP+RV  V+E+ E    ERV  +NG E+   FDP APPPF+L
Sbjct: 61  FCLSSGILREKNWALRVSAPLRVLQVEEEEENKEGERV--INGGEE---FDPGAPPPFKL 120

Query: 121 ADIRAAIPKHCWVKDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWAL 180
           +DIR AIPKHCWVKDPWRSM YVVRDV VVFGLAAVAAYFNNWVVWPLYWFAQ TMFWAL
Sbjct: 121 SDIREAIPKHCWVKDPWRSMGYVVRDVAVVFGLAAVAAYFNNWVVWPLYWFAQSTMFWAL 180

Query: 181 FVLGHDCGHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL 240
           FVLGHDCGHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL
Sbjct: 181 FVLGHDCGHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL 240

Query: 241 SEKIFRSLDKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITS 300
           SEKI+++LD AT+ LRFTLPFP+LAYP+YLWSRSPGK+GSHF P+SDLFVP+E+ D+ITS
Sbjct: 241 SEKIYKNLDTATKKLRFTLPFPLLAYPIYLWSRSPGKQGSHFHPDSDLFVPNEKKDVITS 300

Query: 301 TACWTAMLALLGCLSVVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE- 360
           T CWTAMLALL  LS V+GP+Q+LKLYGIPYL  VMWLD+VTYLHHHGH+DKLPWYRG+ 
Sbjct: 301 TVCWTAMLALLVGLSFVIGPVQLLKLYGIPYLGNVMWLDLVTYLHHHGHEDKLPWYRGKE 360

Query: 361 ---------------------------------------------TEATKPVFGKYYREP 400
                                                        TEA KPV GKYYREP
Sbjct: 361 WSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREP 420

BLAST of Spg028805.1 vs. ExPASy Swiss-Prot
Match: P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 1.9e-164
Identity = 277/445 (62.25%), Postives = 322/445 (72.36%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MA  VLSECG +PLPR YP+ +   FAS      +   PL    +L L S  G  +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPPLKPPSSL-LNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNV+ P+       + + ER            FDP APPPF LADIRAAIPKHCWVK+
Sbjct: 61  WALNVATPLTTLQSPSEEDTER------------FDPGAPPPFNLADIRAAIPKHCWVKN 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E I+++L+K T+  
Sbjct: 181 PRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMF 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL CL+
Sbjct: 241 RFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLN 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
            VMGP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+                
Sbjct: 301 FVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEA KPV GKYYREP+ S PLP HLLG L+
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPKNSGPLPLHLLGSLI 420

BLAST of Spg028805.1 vs. ExPASy Swiss-Prot
Match: P48621 (Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 8.1e-163
Identity = 281/451 (62.31%), Postives = 328/451 (72.73%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVS--EIRSPLLASKALTLRSSLGLSTKG 60
           MA W   +CG+KPL  + P+P      S T  V   +    +   K   LR +L    + 
Sbjct: 1   MATWYHQKCGLKPLAPVIPRPRTGAALSSTSRVEFLDTNKVVAGPKFQPLRCNL----RE 60

Query: 61  KSWALNVSAPVRVASVDEDNER-ERVDGVNGVEDE--GGFDPAAPPPFRLADIRAAIPKH 120
           ++W L VSAP+RVAS++E+ +  +  +G NGVE E    FDP APPPF LADIRAAIPKH
Sbjct: 61  RNWGLKVSAPLRVASIEEEQKSVDLTNGTNGVEHEKLPEFDPGAPPPFNLADIRAAIPKH 120

Query: 121 CWVKDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHG 180
           CWVKDPWRSMSYVVRDV+ VFGLAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCGHG
Sbjct: 121 CWVKDPWRSMSYVVRDVIAVFGLAAAAAYLNNWLVWPLYWAAQGTMFWALFVLGHDCGHG 180

Query: 181 SFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDK 240
           SFSN+ KLNSVVGH+LHSSILVPYHGWRISHRTHHQ+HGH ENDESWHPL EK+FRSLD 
Sbjct: 181 SFSNNSKLNSVVGHLLHSSILVPYHGWRISHRTHHQHHGHAENDESWHPLPEKLFRSLDT 240

Query: 241 ATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLAL 300
            TR LRFT PFP+LA+PVYL+SRSPGK GSHFDP+SDLFVP+ER D+ITSTACW AML L
Sbjct: 241 VTRMLRFTAPFPLLAFPVYLFSRSPGKTGSHFDPSSDLFVPNERKDVITSTACWAAMLGL 300

Query: 301 LGCLSVVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE----------- 360
           L  L  VMGP+Q+LKLYG+PY++FVMWLD+VTYLHHHGH+DKLPWYRG+           
Sbjct: 301 LVGLGFVMGPIQLLKLYGVPYVIFVMWLDLVTYLHHHGHEDKLPWYRGKEWSYLRGGLTT 360

Query: 361 -----------------------------------TEATKPVFGKYYREPEKS-WPLPFH 400
                                              TEA KPVFGKYYREP+KS  PLPFH
Sbjct: 361 LDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVFGKYYREPKKSAAPLPFH 420

BLAST of Spg028805.1 vs. ExPASy Swiss-Prot
Match: P46310 (sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD7 PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-159
Identity = 277/449 (61.69%), Postives = 323/449 (71.94%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRS--SLGLSTKG 60
           MA  VLSECG++PLPRIY  P R+ F S     ++ R  L +S   T  S  S GL+++ 
Sbjct: 1   MANLVLSECGIRPLPRIYTTP-RSNFLSNN---NKFRPSLSSSSYKTSSSPLSFGLNSRD 60

Query: 61  ---KSWALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKH 120
              ++WALNVS P+     +E    E        +++  FDP APPPF LADIRAAIPKH
Sbjct: 61  GFTRNWALNVSTPLTTPIFEESPLEE--------DNKQRFDPGAPPPFNLADIRAAIPKH 120

Query: 121 CWVKDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHG 180
           CWVK+PW+S+SYVVRDV +VF LAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCGHG
Sbjct: 121 CWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHDCGHG 180

Query: 181 SFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDK 240
           SFSNDPKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKI+ +LDK
Sbjct: 181 SFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYNTLDK 240

Query: 241 ATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLAL 300
            TR  RFTLP  MLAYP YLW+RSPGKKGSH+ P+SDLF+P ER D++TSTACWTAM AL
Sbjct: 241 PTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTAMAAL 300

Query: 301 LGCLSVVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE----------- 360
           L CL+  +GP+Q+LKLYGIPY + VMWLD VTYLHHHGH+DKLPWYRG+           
Sbjct: 301 LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTT 360

Query: 361 -----------------------------------TEATKPVFGKYYREPEKSWPLPFHL 399
                                              TEA KPV GKYYREP+KS PLP HL
Sbjct: 361 LDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHL 420

BLAST of Spg028805.1 vs. ExPASy TrEMBL
Match: A0A5A7UH66 (Omega-3 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold186G001460 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 2.9e-224
Identity = 380/445 (85.39%), Postives = 389/445 (87.42%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNGFAS+T MVSEIR PLL SKA  LRSSLG+STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVS PV +ASVDEDNERERVD VNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK++RSLDKATRTL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPYLMFVMWLD VTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPVFGKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. ExPASy TrEMBL
Match: A0A1S3CNS0 (omega-3 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502871 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 2.9e-224
Identity = 380/445 (85.39%), Postives = 389/445 (87.42%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNGFAS+T MVSEIR PLL SKA  LRSSLG+STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVS PV +ASVDEDNERERVD VNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK++RSLDKATRTL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPYLMFVMWLD VTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPVFGKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. ExPASy TrEMBL
Match: B3VA19 (Omega-3 fatty acid desaturase OS=Cucumis sativus OX=3659 GN=fad7 PE=2 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.1e-222
Identity = 376/445 (84.49%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYP PSRNGFAS+T MVSEIR PLLASKA  LRSSLGLSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVS PV VASVDEDNERERV+ VNGVEDEG FDPAAPPPFRLADIRAAIPKHCWV+D
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVEDEGSFDPAAPPPFRLADIRAAIPKHCWVRD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P LNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+RSLDKATRTL
Sbjct: 181 PNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RF LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS
Sbjct: 241 RFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQI+KLYGIPY+MFVMWLD VTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQIIKLYGIPYMMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKP+FGKYYREPEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVVHHLFPQIPHYHLIEATEATKPIFGKYYREPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. ExPASy TrEMBL
Match: A0A6J1K2M7 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491157 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 3.8e-216
Identity = 372/445 (83.60%), Postives = 382/445 (85.84%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVKPLPRIYP+PSRNG ASRT MVSEIR   + SK   LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPRPSRNGLASRTYMVSEIRFSSVPSKPNFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVSAP RVASVDEDNE+++VD   GVE+EGGFDPAA PPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSAPARVASVDEDNEKDKVD---GVENEGGFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFR+LDKATRTL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRTLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
           VVMGPLQILKLYGIPY+MFVMWLDVVTYLHHHGHDDKLPWYRGE                
Sbjct: 301 VVMGPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPV GKYYR+PEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. ExPASy TrEMBL
Match: A0A6J1FSG4 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447744 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 368/445 (82.70%), Postives = 383/445 (86.07%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MARWVLSECGVK LPRIYP+PSRNG ASRTCMVSEIRS  + SK   LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNVSAPVRVASVDEDNE+++VD   GVE+EGGFDPAA PPFRLADIRAAIPKHCWVKD
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVD---GVENEGGFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+++LDKAT+TL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATKTL 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYP+YLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGCLS
Sbjct: 241 RFTLPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLS 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
            VMGPLQILKLYGIPY+MFVMWLDVVTYLHHHGHDDKLPWYRGE                
Sbjct: 301 AVMGPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEATKPV GKYYR+PEKSWPLPFHLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLV 420

BLAST of Spg028805.1 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 580.1 bits (1494), Expect = 1.4e-165
Identity = 277/445 (62.25%), Postives = 322/445 (72.36%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MA  VLSECG +PLPR YP+ +   FAS      +   PL    +L L S  G  +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPPLKPPSSL-LNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNV+ P+       + + ER            FDP APPPF LADIRAAIPKHCWVK+
Sbjct: 61  WALNVATPLTTLQSPSEEDTER------------FDPGAPPPFNLADIRAAIPKHCWVKN 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E I+++L+K T+  
Sbjct: 181 PRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMF 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL CL+
Sbjct: 241 RFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLN 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE---------------- 360
            VMGP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+                
Sbjct: 301 FVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDY 360

Query: 361 ------------------------------TEATKPVFGKYYREPEKSWPLPFHLLGVLV 400
                                         TEA KPV GKYYREP+ S PLP HLLG L+
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPKNSGPLPLHLLGSLI 420

BLAST of Spg028805.1 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 564.3 bits (1453), Expect = 7.8e-161
Identity = 277/449 (61.69%), Postives = 323/449 (71.94%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRS--SLGLSTKG 60
           MA  VLSECG++PLPRIY  P R+ F S     ++ R  L +S   T  S  S GL+++ 
Sbjct: 1   MANLVLSECGIRPLPRIYTTP-RSNFLSNN---NKFRPSLSSSSYKTSSSPLSFGLNSRD 60

Query: 61  ---KSWALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKH 120
              ++WALNVS P+     +E    E        +++  FDP APPPF LADIRAAIPKH
Sbjct: 61  GFTRNWALNVSTPLTTPIFEESPLEE--------DNKQRFDPGAPPPFNLADIRAAIPKH 120

Query: 121 CWVKDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHG 180
           CWVK+PW+S+SYVVRDV +VF LAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCGHG
Sbjct: 121 CWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHDCGHG 180

Query: 181 SFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDK 240
           SFSNDPKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKI+ +LDK
Sbjct: 181 SFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYNTLDK 240

Query: 241 ATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLAL 300
            TR  RFTLP  MLAYP YLW+RSPGKKGSH+ P+SDLF+P ER D++TSTACWTAM AL
Sbjct: 241 PTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTAMAAL 300

Query: 301 LGCLSVVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE----------- 360
           L CL+  +GP+Q+LKLYGIPY + VMWLD VTYLHHHGH+DKLPWYRG+           
Sbjct: 301 LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTT 360

Query: 361 -----------------------------------TEATKPVFGKYYREPEKSWPLPFHL 399
                                              TEA KPV GKYYREP+KS PLP HL
Sbjct: 361 LDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHL 420

BLAST of Spg028805.1 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.2e-145
Identity = 238/344 (69.19%), Postives = 275/344 (79.94%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGFASRTCMVSEIRSPLLASKALTLRSSLGLSTKGKS 60
           MA  VLSECG +PLPR YP+ +   FAS      +   PL    +L L S  G  +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPPLKPPSSL-LNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120
           WALNV+ P+       + + ER            FDP APPPF LADIRAAIPKHCWVK+
Sbjct: 61  WALNVATPLTTLQSPSEEDTER------------FDPGAPPPFNLADIRAAIPKHCWVKN 120

Query: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180
           PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 121 PWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTL 240
           P+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E I+++L+K T+  
Sbjct: 181 PRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMF 240

Query: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300
           RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL CL+
Sbjct: 241 RFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLN 300

Query: 301 VVMGPLQILKLYGIPYLMFVMWLDVVTYLHHHGHDDKLPWYRGE 345
            VMGP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+
Sbjct: 301 FVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGK 330

BLAST of Spg028805.1 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 460.3 bits (1183), Expect = 1.6e-129
Identity = 220/370 (59.46%), Postives = 261/370 (70.54%), Query Frame = 0

Query: 78  NERERVDGVNGVED---EGGFDPAAPPPFRLADIRAAIPKHCWVKDPWRSMSYVVRDVVV 137
           ++R  V+G  G  D   E  FDP+A PPF++ DIRAAIPKHCWVK P RSMSYVVRD++ 
Sbjct: 6   DQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIA 65

Query: 138 VFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSS 197
           V  LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGHILHS 
Sbjct: 66  VAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 125

Query: 198 ILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTLRFTLPFPMLAYPVY 257
           ILVPYHGWRISHRTHHQNHGHVENDESW PL E++++ L  +TR LR+T+P PMLAYP+Y
Sbjct: 126 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLY 185

Query: 258 LWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLSVVMGPLQILKLYGI 317
           L  RSPGK+GSHF+P S LF PSER  I TST CW+ M   L  LS V GPL +LK+YG+
Sbjct: 186 LCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGV 245

Query: 318 PYLMFVMWLDVVTYLHHHGHDDKLPWYRGE------------------------------ 377
           PY++FVMWLD VTYLHHHGHD+KLPWYRG+                              
Sbjct: 246 PYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHV 305

Query: 378 ----------------TEATKPVFGKYYREPEKSWPLPFHLLGVLVKSMKKDHYVSDSGD 399
                           T+A K V G+YYREP+ S  +P HL+  LV S+KKDHYVSD+GD
Sbjct: 306 IHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGD 365

BLAST of Spg028805.1 vs. TAIR 10
Match: AT2G29980.2 (fatty acid desaturase 3 )

HSP 1 Score: 407.9 bits (1047), Expect = 9.3e-114
Identity = 186/275 (67.64%), Postives = 218/275 (79.27%), Query Frame = 0

Query: 78  NERERVDGVNGVED---EGGFDPAAPPPFRLADIRAAIPKHCWVKDPWRSMSYVVRDVVV 137
           ++R  V+G  G  D   E  FDP+A PPF++ DIRAAIPKHCWVK P RSMSYVVRD++ 
Sbjct: 6   DQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIA 65

Query: 138 VFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSS 197
           V  LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGHILHS 
Sbjct: 66  VAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 125

Query: 198 ILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRSLDKATRTLRFTLPFPMLAYPVY 257
           ILVPYHGWRISHRTHHQNHGHVENDESW PL E++++ L  +TR LR+T+P PMLAYP+Y
Sbjct: 126 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLY 185

Query: 258 LWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLSVVMGPLQILKLYGI 317
           L  RSPGK+GSHF+P S LF PSER  I TST CW+ M   L  LS V GPL +LK+YG+
Sbjct: 186 LCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGV 245

Query: 318 PYLMFVMWLDVVTYLHHHGHDDKLPWYRGETEATK 350
           PY++FVMWLD VTYLHHHGHD+KLPWYRG+    K
Sbjct: 246 PYIIFVMWLDAVTYLHHHGHDEKLPWYRGKVSKIK 280