Spg028522.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg028522.1 vs. NCBI nr
Match: XP_022990941.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022990948.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 663/764 (86.78%), Postives = 705/764 (92.28%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREV L LSI LATS AA+DQQ+YIIHMDTTKMA  +PEQWY ++IDS+NKISSLD  
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLDDQ 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           EEAS+AA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWNSSNL SDI+IG+LDTGIWPEHISF+DKGLPPVPKKWKG CQAG  FS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           +KL+GA AYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIV KAS +NQ MGV
Sbjct: 181 RKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           ATGMRFTSRIAAYKVCW +GCANADILAAIDRAV DGVDVLSLSLGG ASAFY+DDIAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
           TFGAVRNGVFVSCSAGNSGP +STV N+APWIMT+AASYTDR FPA VKLGNGQV EGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 437
           LYSG +I QLPLVYNNTAGG+EANVCTAGSLVPS VKGKIVVCERGTNSRTAKGEQVKLA
Sbjct: 361 LYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 438 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 497
           GGAGMILINTQLEGEELFADPHVLPA  LGASAG+AII YI+SSKHQPKA I FEGTK+G
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 498 SRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSG 557
           +RAPRVAAFSSRGPS++  DVIKPD+TAPGVNILAAWP I SPSELESDKRRVLFNI+SG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNIISG 540

Query: 558 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFA 617
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDN+MSPISDVGS +GKPANPFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANPFA 600

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FGSGHVDPE ASDPGLIYDITP+DYLNY CSLNYNSTQI LVSRGNFTCPSKR   QPG+
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQPGN 660

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSFS FMKKKAKN +VT KRTVTNVG PRSDYTV+I NPKG+ I V+PEKLSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFRRYG 720

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +KLSYQVSFVALGKRE L  FSFGSLVW+SGKY VRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Spg028522.1 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1323.9 bits (3425), Expect = 0.0e+00
Identity = 660/764 (86.39%), Postives = 704/764 (92.15%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSL--D 77
           MGFREV L LSI LATSTAA+DQQ+YIIHMDTTKMA   PEQWY ++IDS+N+ISSL   
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLHDQ 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           +EASNAA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  QEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWNSSNL SDI+IG+LDTGIWPEHISF+DKGLPPVPKKWKG CQ G  FS SNCN
Sbjct: 121 QRQHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQPGQKFSPSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           +KL+GA AYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIV KAS +NQ MGV
Sbjct: 181 RKLIGAMAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           ATGMRFTSRIAAYKVCW +GCANADILAAIDRAV DGVDVLSLSLGG ASAFY+DDIAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
           TFGAVRNGVFVSCSAGNSGP +STV N+APWIMT+AASYTDR FPA VKLGNGQV EGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 437
           LY+G NI QLPLVYNNTAGGQEANVCT GSLVPS VKGKIVVCERGTNSRTAKGEQVKLA
Sbjct: 361 LYTGNNIGQLPLVYNNTAGGQEANVCTPGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 438 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 497
           GGAGMILINTQLEGEELFADPHVLPA  LGASAG+AII YI+SSKHQPKA I FEGTK+G
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIGFEGTKFG 480

Query: 498 SRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSG 557
           +RAPRVAAFSSRGPS++  DVIKPD+TAPGVNILAAWP I SPSELESDKRRVLFN++SG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNVISG 540

Query: 558 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFA 617
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDN+MSPISDVGS +GKPANPFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFA 600

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FGSGHVDPE ASDPGLIYDITP+DYL+Y CSLNYNSTQI LVSRGNFTCPSKR   QPG+
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLDYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQPGN 660

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSFS FMKKKAKN +VT KRTVTNVG PRSDY+V+I NPKG+ I VKPEKLSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGGPRSDYSVKIKNPKGIVISVKPEKLSFRRYG 720

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +KLSYQVSFVALGKRE +S FSFGSLVW+SGKYAVRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGVSGFSFGSLVWVSGKYAVRSPIAVTWK 764

BLAST of Spg028522.1 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1323.9 bits (3425), Expect = 0.0e+00
Identity = 661/764 (86.52%), Postives = 706/764 (92.41%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREV L LSI LATSTAA+DQQ+YIIHMDTTKMA   PEQWY ++IDS+N+ISSL+  
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLEDQ 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           EEASNAA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWNSSNL SDI+IG+LDTGIWPEHISF+DKGLPPVPKKWKG CQAG  FS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           +KL+GA AYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIV KAS +NQ MGV
Sbjct: 181 RKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           ATGMRFTSRIAAYKVCW +GCANADILAAIDRAV DGVDVLSLSLGG ASAFY+DDIAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
           TFGAVRNGVFVSCSAGNSGP +STV N+APWIMT+AASYTDR+FPA VKLGNGQV EGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 437
           LYSG NI QLPLVYNNTAGG++ANVCTAGSLVPS VKGKIVVCERGTNSRTAKGEQVKLA
Sbjct: 361 LYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 438 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 497
           GGAGMILINTQLEGEELFADPHVLPA  LGASAG+AII YI+SSKHQPKA I FEGTK+G
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 498 SRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSG 557
           +RAPRVAAFSSRGPS++  DVIKPD+TAPGVNILAAWP I SPSE+ESDKRRVLFN++SG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVISG 540

Query: 558 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFA 617
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDN+MSPISDVGS +GKPANPFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFA 600

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FGSGHVDPE ASDPGLIYDITP+DYLNYLCSLNYNSTQI LVSRGNFTCPSKR   Q G 
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQAGK 660

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSFS FMKKKAKN +VT KRTVTNVG PRSDY+V+I NPKG+ I VKPEKLSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFRRYG 720

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +KLSYQVSFVALGKRE +S FSFGSLVW+SG YAVRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Spg028522.1 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 647/767 (84.35%), Postives = 697/767 (90.87%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREVW+ LSI+LA +TAA+DQQTYIIHMDTTKM TTNPEQWY +MIDS+N++ SLD  
Sbjct: 1   MGFREVWMFLSIMLAIATAAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLDDE 60

Query: 78  --EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 137
             EEAS+ AEILYVYKTA+SGFAAKLS +KL SLSKIPGFLAATP+ELLQLHTTHSPQFL
Sbjct: 61  NKEEASDTAEILYVYKTALSGFAAKLSRKKLDSLSKIPGFLAATPNELLQLHTTHSPQFL 120

Query: 138 GLQRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSN 197
           GL+R HGLWNSSNL SDIIIG+LDTGIWPEHISF+DKGL PVP KWKGICQAGP FS SN
Sbjct: 121 GLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKWKGICQAGPKFSPSN 180

Query: 198 CNKKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGM 257
           CNKKL+GA AYIKGYEAIVG LN TGTFRSPRDSDGHGTHTASTAAG++V KAS +NQGM
Sbjct: 181 CNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAAGSMVNKASFFNQGM 240

Query: 258 GVATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIA 317
           GVATGM +TSRIAAYKVCWP GCANADILAA+D AV DGVDVLSLSLGGGA +FYRD+IA
Sbjct: 241 GVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIA 300

Query: 318 IATFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEG 377
           IA FGA++NGVFVSCSAGNSGPF STVGN APWIMT+AASYTDR FP  VKLGNGQV EG
Sbjct: 301 IAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEG 360

Query: 378 SSLYSGKNINQLPLVYNNTAG-GQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQV 437
           SSLY GKNIN LPLVYNNTAG GQE N CTAGSL P+ VKGKIV+CERG+NSRT KGEQV
Sbjct: 361 SSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLCERGSNSRTDKGEQV 420

Query: 438 KLAGGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGT 497
           KLAGGAGMILINTQ EGEELFADPHVLPATTLGASAGKAI++YIASSK Q KAS+ FEGT
Sbjct: 421 KLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKSQAKASMAFEGT 480

Query: 498 KYGSRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNI 557
           KYGSRAPRVAAFSSRGPS VG DV+KPD+TAPGVNILAAWPPIVSPSEL+SDKRRVLFNI
Sbjct: 481 KYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSPSELDSDKRRVLFNI 540

Query: 558 VSGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPAN 617
           +SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAY  DNKM+ +SDVG  +G PA+
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMNLVSDVGHPSGGPAD 600

Query: 618 PFAFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQ 677
           PFAFGSGHVDPE ASDPGL+YDITP+DY+NYLCSL YNSTQIALVSRGNFTC SKRT LQ
Sbjct: 601 PFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTLLQ 660

Query: 678 PGDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFR 737
           P DLNYPSFS FMKKKAKN ++T KRTVTNVG PRSDYTV+INNPKG+ I VKPEKLSF 
Sbjct: 661 PRDLNYPSFSVFMKKKAKNVSITSKRTVTNVGIPRSDYTVKINNPKGIRITVKPEKLSFG 720

Query: 738 RLGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
            LGEKLS+QVSFVALG +EALS+FSFG LVWLSGKYAVRSPIAVTWQ
Sbjct: 721 SLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTWQ 767

BLAST of Spg028522.1 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 642/766 (83.81%), Postives = 695/766 (90.73%), Query Frame = 0

Query: 18  MGFREVW-LLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLDE 77
           MG  E+W LLLSI+LATS AA+DQQTYIIHMD TKMATTNPEQWY S+I S+N++SS+++
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 78  ---EASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 137
              EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFLAATPD+LLQLHTTH+PQFL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 138 GLQRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSN 197
           GLQRGHGLWN+SNL SDIIIGV+DTGIWPEHISF+DKGLPPVPKKWKG CQAGP FSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 198 CNKKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGM 257
           CNKKLVGARAYIKGYE I+GRLN TGTFRS RDSDGHGTHTASTAAGNIVYKASLYNQGM
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 258 GVATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIA 317
           G ATGMRFTSRIAAYKVCWP+GCA+ DILAAIDRAVVDGVDVLSLSLGGG   FY+D+IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 318 IATFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEG 377
           IA FGAVRNGVFVSCSAGNSGPF STVGNVAPWIMT+AASYTDR FP  VKLGNGQ+ EG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 378 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVK 437
           SSL+SG +I +LPLVYN TAGG+EA+VCTAGSLVPS VKGKIVVCERGTNSR  KGEQVK
Sbjct: 361 SSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 438 LAGGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTK 497
           LAGG GMILINTQLEGEELF D HVLPA  LGASAGKAIINYIASSKH PKASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGTR 480

Query: 498 YGSRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIV 557
           YGSRAPR+AAFSSRGPS     VIKPDITAPGVNILAAWPP+VSPSEL+SDKRRVLFNI+
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 558 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANP 617
           SGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAY NDNK S ISDVG  +G PA+P
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADP 600

Query: 618 FAFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQP 677
           +AFGSGHVDPE A DPGL+YDI P+DYLNYLCSLNY S Q+ LVSRGNF+CPSKRT LQP
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQP 660

Query: 678 GDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRR 737
           GDLNYPSFS  MK KAKN  V FKRTVTNVG+P SDYTV+INNP G+ + VKPEKLSFRR
Sbjct: 661 GDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFRR 720

Query: 738 LGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
            G+KLSYQVSFVALGKRE LS+FSFGSLVW+SGKY+VRSPIAV W+
Sbjct: 721 SGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Spg028522.1 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 1.8e-243
Identity = 427/764 (55.89%), Postives = 551/764 (72.12%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLDEE 77
           M FR   +   ++   S  +  +QTY+IH       TT+ +    S+ +S+ +  +++++
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENINDD 78

Query: 78  ASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR 137
             +  EI Y+Y+ A+SGF+A L+  +L ++    GF++A PDELL LHTT+S +FLGL+ 
Sbjct: 79  DFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEF 138

Query: 138 GHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKK 197
           G GLWN ++L SD+IIG++DTGI PEH+SFRD  + PVP +W+G C  G NFS S CNKK
Sbjct: 139 GIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKK 198

Query: 198 LVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVAT 257
           ++GA A+ KGYE+IVG++N T  FRS RD+ GHGTHTASTAAG+IV KA+ + Q  G+A+
Sbjct: 199 IIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLAS 258

Query: 258 GMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATF 317
           GMRFTSRIAAYK CW  GCA+ D++AAIDRA++DGVDV+SLSLGG +  FY D IAIA F
Sbjct: 259 GMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGF 318

Query: 318 GAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLY 377
           GA++  +FVSCSAGNSGP  STV N APW+MT+AASYTDR FPA+V++GN + L GSSLY
Sbjct: 319 GAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLY 378

Query: 378 SGKNINQLPLVYNNTAGGQEANV-CTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAG 437
            GK++  LPL +N TAG +   V C   SL    V+GKIV+C RG + RTAKGE+VK +G
Sbjct: 379 KGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSG 438

Query: 438 GAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGS 497
           GA M+L++T+ EGEEL ADPHVLPA +LG S GK ++NY+A + +   AS+ F GT YG+
Sbjct: 439 GAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYGA 498

Query: 498 RAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSGT 557
            AP VAAFSSRGPS+ G ++ KPDI APG+NILA W P  SPS L SD RRV FNI+SGT
Sbjct: 499 TAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGT 558

Query: 558 SMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGK-PANPFA 617
           SM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA   DN+  PI D G+   +  A  FA
Sbjct: 559 SMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFA 618

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FG+G+VDP  A DPGL+YD +  DYLNYLCSLNY S +I L S  N+TC S    L PGD
Sbjct: 619 FGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGD 678

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSF+  +   A   TV +KRTVTNVGSP  +Y V +  PKGV + V+P+ L F++  
Sbjct: 679 LNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKAR 738

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           E+LSY V++ A   R + S  SFG LVW+  KY VRSPIAVTW+
Sbjct: 739 ERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Spg028522.1 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.1e-200
Identity = 371/753 (49.27%), Postives = 501/753 (66.53%), Query Frame = 0

Query: 34  STAALDQQTYIIHMDTTKMATTNP--EQWYASMIDSVNKISSLDEEASNAAEILYVYKTA 93
           S+++ DQ TYI+HM  ++M ++      WY S + S+          S++AE+LY Y+ A
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI----------SDSAELLYTYENA 82

Query: 94  ISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL-QRGHGLWNSSNLGSD 153
           I GF+ +L+  +  SL   PG ++  P+   +LHTT +P FLGL +    L+  +   SD
Sbjct: 83  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 142

Query: 154 IIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGARAYIKGYEA 213
           +++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG NF+ S CN+KL+GAR + +GYE+
Sbjct: 143 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 202

Query: 214 IVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRFTSRIAAYKV 273
            +G ++ +   RSPRD DGHGTHT+STAAG++V  ASL     G A GM   +R+A YKV
Sbjct: 203 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 262

Query: 274 CWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVRNGVFVSCSA 333
           CW  GC ++DILAAID+A+ D V+VLS+SLGGG S +YRD +AI  F A+  G+ VSCSA
Sbjct: 263 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 322

Query: 334 GNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKNI--NQLPLV 393
           GN+GP +S++ NVAPWI T+ A   DR FPA+  LGNG+   G SL+ G+ +    LP +
Sbjct: 323 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 382

Query: 394 Y-NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQL 453
           Y  N +     N+C  G+L+P KVKGKIV+C+RG N+R  KG+ VK AGG GMIL NT  
Sbjct: 383 YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAA 442

Query: 454 EGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGSR-APRVAAFSS 513
            GEEL AD H+LPATT+G  AG  I +Y+ +  + P ASI+  GT  G + +P VAAFSS
Sbjct: 443 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFSS 502

Query: 514 RGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSGTSMSCPHVSGL 573
           RGP+ +  +++KPD+ APGVNILAAW     P+ L SD RRV FNI+SGTSMSCPHVSGL
Sbjct: 503 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 562

Query: 574 AALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFAFGSGHVDPENA 633
           AALLKS H +WSPAAI+SALMTTAY       P+ D+ +  GKP+ PF  G+GHV P  A
Sbjct: 563 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTA 622

Query: 634 SDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGDLNYPSFSAFMK 693
           ++PGLIYD+T EDYL +LC+LNY S QI  VSR N+TC   +++    DLNYPSF+  + 
Sbjct: 623 TNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY-SVADLNYPSFAVNVD 682

Query: 694 KKAKNFTVTFKRTVTNVGSPRSDYTVQI-NNPKGVTIIVKPEKLSFRRLGEKLSYQVSFV 753
                    + RTVT+VG     Y+V++ +   GV I V+P  L+F+   EK SY V+F 
Sbjct: 683 GVG---AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT 742

Query: 754 ALGKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
               + + S  SFGS+ W  GK+ V SP+A++W
Sbjct: 743 VDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Spg028522.1 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.2e-199
Identity = 381/768 (49.61%), Postives = 494/768 (64.32%), Query Frame = 0

Query: 32  ATSTAALDQQTYIIHMDTTKMATTNPE--QWYASMIDSVNKISSLDEEASNAAEILYVYK 91
           ++S ++ +  TYI+H+D     +  P    WY S + S+          S+   I++ Y 
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL---------TSSPPSIIHTYD 76

Query: 92  TAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGH--GLWNSSNL 151
           T   GF+A+L+++    L   P  ++  P+++  LHTT SP+FLGL+     GL   S+ 
Sbjct: 77  TVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDF 136

Query: 152 GSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGARAYIKG 211
           GSD++IGV+DTG+WPE  SF D+GL PVP KWKG C A  +F  S CN+KLVGAR +  G
Sbjct: 137 GSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGG 196

Query: 212 YEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRFTSRIAA 271
           YEA  G++N T  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA GM   +R+AA
Sbjct: 197 YEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAA 256

Query: 272 YKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVRNGVFVS 331
           YKVCW  GC ++DILAA D AV DGVDV+SLS+GG    +Y D IAI  FGA+  G+FVS
Sbjct: 257 YKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVS 316

Query: 332 CSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKNIN---Q 391
            SAGN GP   TV NVAPW+ T+ A   DR FPA VKLGNG+++ G S+Y G  ++    
Sbjct: 317 ASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRM 376

Query: 392 LPLVYNNT---AGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAGGAGMI 451
            PLVY  +     G  +++C  GSL P+ VKGKIV+C+RG NSR  KGE V+  GG GMI
Sbjct: 377 YPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMI 436

Query: 452 LINTQLEGEELFADPHVLPATTLGASAGKAIINYIA------SSKHQPKASITFEGTKYG 511
           + N   +GE L AD HVLPAT++GAS G  I  YI+      SSKH P A+I F+GT+ G
Sbjct: 437 IANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKH-PTATIVFKGTRLG 496

Query: 512 SR-APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 571
            R AP VA+FS+RGP+    +++KPD+ APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 497 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 556

Query: 572 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 631
           GTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAYT DN   P+ D   + G  ++  
Sbjct: 557 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 616

Query: 632 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPG 691
            +GSGHV P  A DPGL+YDIT  DY+N+LC+ NY  T I  ++R    C   R     G
Sbjct: 617 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 676

Query: 692 DLNYPSFS-AFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRR 751
           +LNYPSFS  F +      +  F RTVTNVG   S Y ++I  P+G T+ V+PEKLSFRR
Sbjct: 677 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 736

Query: 752 LGEKLSY--QVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +G+KLS+  +V    +      +    G +VW  GK  V SP+ VT Q
Sbjct: 737 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Spg028522.1 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 2.8e-199
Identity = 370/766 (48.30%), Postives = 501/766 (65.40%), Query Frame = 0

Query: 26  LLSIVLATSTAALDQQTYIIHMDTTKMAT--TNPEQWYASMIDSVNKISSLDEEASNAAE 85
           L+ +   T+T    ++TY+IHMD + M    TN  QWY+S I+SV +  S +EE +N   
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNN-NR 79

Query: 86  ILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR--GHGL 145
           ILY Y+TA  G AA+L+  +   L +  G +A  P+   +LHTT SP FLGL+R     +
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 146 WNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGA 205
           W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G  F + NCN+K+VGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 206 RAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRF 265
           R + +GYEA  G+++    ++SPRD DGHGTHTA+T AG+ V  A+L+    G A GM  
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 266 TSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVR 325
            +R+AAYKVCW  GC ++DIL+A+D+AV DGV VLS+SLGGG S + RD ++IATFGA+ 
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAME 319

Query: 326 NGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKN 385
            GVFVSCSAGN GP   ++ NV+PWI T+ AS  DR FPA VK+G  +  +G SLY G+ 
Sbjct: 320 MGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRT 379

Query: 386 I----NQLPLVY--NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 445
           +     Q PLVY   N +     + C  G+L    V GKIV+C+RG   R  KG+ VK A
Sbjct: 380 VLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRA 439

Query: 446 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 505
           GG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +SK +  AS+   GT+ G
Sbjct: 440 GGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGTRIG 499

Query: 506 SR-APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 565
            + +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW   ++PS L SD RRV FNI+S
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 566 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 625
           GTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY +DN   P++D  ++   P++P+
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD--ASGAAPSSPY 619

Query: 626 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSR-GNFTCPSKRTFLQ- 685
             G+GH+DP  A+DPGL+YDI P++Y  +LC+ + + +Q+ + ++  N TC  K T  + 
Sbjct: 620 DHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAKN 679

Query: 686 PGDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFR 745
           PG+LNYP+ SA   +      +T +RTVTNVG   S Y V ++  KG ++ V+P+ L+F 
Sbjct: 680 PGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFT 739

Query: 746 RLGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
              +KLSY V+F     R  +    FG LVW S  + VRSP+ +TW
Sbjct: 740 SKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Spg028522.1 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.9e-193
Identity = 369/770 (47.92%), Postives = 502/770 (65.19%), Query Frame = 0

Query: 25  LLLSIVLATSTAALDQQTYIIHMDTTKMAT--TNPEQWYASMIDSVNKISSLDEEASNAA 84
           LLL     +S+++   ++YI+H+  +   +  ++   W+ S++ S+         +   A
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL-------PSSPQPA 74

Query: 85  EILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGHGLW 144
            +LY Y  A+ GF+A+LS  +  +L + P  ++  PD+  ++HTTH+P FLG  +  GLW
Sbjct: 75  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLW 134

Query: 145 NSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGAR 204
           ++SN G D+I+GVLDTGIWPEH SF D GL P+P  WKG C+ GP+F  S+CN+KL+GAR
Sbjct: 135 SNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGAR 194

Query: 205 AYIKGY--EAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMR 264
           A+ +GY  +    + +A    RSPRD++GHGTHTASTAAG++V  ASLY    G ATGM 
Sbjct: 195 AFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMA 254

Query: 265 FTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLG--GGASAFYRDDIAIATFG 324
             +RIAAYK+CW  GC ++DILAA+D+AV DGV V+SLS+G  G A  ++ D IAI  FG
Sbjct: 255 SKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFG 314

Query: 325 AVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYS 384
           A R+G+ VSCSAGNSGP   T  N+APWI+T+ AS  DR F A    G+G+V  G+SLY+
Sbjct: 315 ATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYA 374

Query: 385 GKNI--NQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAG 444
           G+++  +QL LVY+   G   + +C  G L  S V+GKIV+C+RG N+R  KG  VKLAG
Sbjct: 375 GESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAG 434

Query: 445 GAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGS 504
           GAGMIL NT   GEEL AD H++PAT +GA AG  I +YI +S   P A I+F GT  G 
Sbjct: 435 GAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIGP 494

Query: 505 R--APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 564
              +PRVAAFSSRGP+ +   ++KPD+ APGVNILA W  +V P++L+ D RRV FNI+S
Sbjct: 495 SPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIIS 554

Query: 565 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 624
           GTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N   PI D+ +  GK +N F
Sbjct: 555 GTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLAT--GKSSNSF 614

Query: 625 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRT--FLQ 684
             G+GHVDP  A +PGL+YDI  ++Y+ +LC++ Y    I +  +      +  T     
Sbjct: 615 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRT 674

Query: 685 PGDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGS-PRSDYTVQINNPKGVTIIVKPEKLSF 744
            GDLNYPSFS       +   V +KR V NVGS   + Y V + +P  V I V P KL+F
Sbjct: 675 AGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 734

Query: 745 RRLGEKLSYQVSFVAL---GKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
            +    L Y+V+F ++   G   ++    FGS+ W  G++ V+SP+AV W
Sbjct: 735 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Spg028522.1 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 663/764 (86.78%), Postives = 705/764 (92.28%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREV L LSI LATS AA+DQQ+YIIHMDTTKMA  +PEQWY ++IDS+NKISSLD  
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLDDQ 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           EEAS+AA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWNSSNL SDI+IG+LDTGIWPEHISF+DKGLPPVPKKWKG CQAG  FS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           +KL+GA AYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIV KAS +NQ MGV
Sbjct: 181 RKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           ATGMRFTSRIAAYKVCW +GCANADILAAIDRAV DGVDVLSLSLGG ASAFY+DDIAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
           TFGAVRNGVFVSCSAGNSGP +STV N+APWIMT+AASYTDR FPA VKLGNGQV EGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 437
           LYSG +I QLPLVYNNTAGG+EANVCTAGSLVPS VKGKIVVCERGTNSRTAKGEQVKLA
Sbjct: 361 LYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 438 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 497
           GGAGMILINTQLEGEELFADPHVLPA  LGASAG+AII YI+SSKHQPKA I FEGTK+G
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 498 SRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSG 557
           +RAPRVAAFSSRGPS++  DVIKPD+TAPGVNILAAWP I SPSELESDKRRVLFNI+SG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNIISG 540

Query: 558 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFA 617
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDN+MSPISDVGS +GKPANPFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANPFA 600

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FGSGHVDPE ASDPGLIYDITP+DYLNY CSLNYNSTQI LVSRGNFTCPSKR   QPG+
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQPGN 660

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSFS FMKKKAKN +VT KRTVTNVG PRSDYTV+I NPKG+ I V+PEKLSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFRRYG 720

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +KLSYQVSFVALGKRE L  FSFGSLVW+SGKY VRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Spg028522.1 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1323.9 bits (3425), Expect = 0.0e+00
Identity = 661/764 (86.52%), Postives = 706/764 (92.41%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREV L LSI LATSTAA+DQQ+YIIHMDTTKMA   PEQWY ++IDS+N+ISSL+  
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLEDQ 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           EEASNAA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWNSSNL SDI+IG+LDTGIWPEHISF+DKGLPPVPKKWKG CQAG  FS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           +KL+GA AYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIV KAS +NQ MGV
Sbjct: 181 RKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           ATGMRFTSRIAAYKVCW +GCANADILAAIDRAV DGVDVLSLSLGG ASAFY+DDIAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
           TFGAVRNGVFVSCSAGNSGP +STV N+APWIMT+AASYTDR+FPA VKLGNGQV EGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 437
           LYSG NI QLPLVYNNTAGG++ANVCTAGSLVPS VKGKIVVCERGTNSRTAKGEQVKLA
Sbjct: 361 LYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 438 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 497
           GGAGMILINTQLEGEELFADPHVLPA  LGASAG+AII YI+SSKHQPKA I FEGTK+G
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 498 SRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSG 557
           +RAPRVAAFSSRGPS++  DVIKPD+TAPGVNILAAWP I SPSE+ESDKRRVLFN++SG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVISG 540

Query: 558 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFA 617
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDN+MSPISDVGS +GKPANPFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFA 600

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FGSGHVDPE ASDPGLIYDITP+DYLNYLCSLNYNSTQI LVSRGNFTCPSKR   Q G 
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQAGK 660

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSFS FMKKKAKN +VT KRTVTNVG PRSDY+V+I NPKG+ I VKPEKLSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFRRYG 720

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +KLSYQVSFVALGKRE +S FSFGSLVW+SG YAVRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Spg028522.1 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 642/766 (83.81%), Postives = 695/766 (90.73%), Query Frame = 0

Query: 18  MGFREVW-LLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLDE 77
           MG  E+W LLLSI+LATS AA+DQQTYIIHMD TKMATTNPEQWY S+I S+N++SS+++
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 78  ---EASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 137
              EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFLAATPD+LLQLHTTH+PQFL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 138 GLQRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSN 197
           GLQRGHGLWN+SNL SDIIIGV+DTGIWPEHISF+DKGLPPVPKKWKG CQAGP FSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 198 CNKKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGM 257
           CNKKLVGARAYIKGYE I+GRLN TGTFRS RDSDGHGTHTASTAAGNIVYKASLYNQGM
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 258 GVATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIA 317
           G ATGMRFTSRIAAYKVCWP+GCA+ DILAAIDRAVVDGVDVLSLSLGGG   FY+D+IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 318 IATFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEG 377
           IA FGAVRNGVFVSCSAGNSGPF STVGNVAPWIMT+AASYTDR FP  VKLGNGQ+ EG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 378 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVK 437
           SSL+SG +I +LPLVYN TAGG+EA+VCTAGSLVPS VKGKIVVCERGTNSR  KGEQVK
Sbjct: 361 SSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 438 LAGGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTK 497
           LAGG GMILINTQLEGEELF D HVLPA  LGASAGKAIINYIASSKH PKASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGTR 480

Query: 498 YGSRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIV 557
           YGSRAPR+AAFSSRGPS     VIKPDITAPGVNILAAWPP+VSPSEL+SDKRRVLFNI+
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 558 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANP 617
           SGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAY NDNK S ISDVG  +G PA+P
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADP 600

Query: 618 FAFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQP 677
           +AFGSGHVDPE A DPGL+YDI P+DYLNYLCSLNY S Q+ LVSRGNF+CPSKRT LQP
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQP 660

Query: 678 GDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRR 737
           GDLNYPSFS  MK KAKN  V FKRTVTNVG+P SDYTV+INNP G+ + VKPEKLSFRR
Sbjct: 661 GDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFRR 720

Query: 738 LGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
            G+KLSYQVSFVALGKRE LS+FSFGSLVW+SGKY+VRSPIAV W+
Sbjct: 721 SGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Spg028522.1 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 637/765 (83.27%), Postives = 689/765 (90.07%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLD-- 77
           MGFREVW +LSI+LA S+A +DQQTYIIHMDTTKM T NPEQWY  +IDSVN++SSLD  
Sbjct: 1   MGFREVW-VLSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN 60

Query: 78  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 137
           EEASNAAEILYVYKTA+SGFAAKL+++KLHSLSKIPGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 138 QRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCN 197
           QR HGLWN SNL SDIIIG+LDTGIWPEHISF+DKGL  VP KWKGICQ GP FS SNCN
Sbjct: 121 QRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCN 180

Query: 198 KKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGV 257
           KKL+GA AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAG+IV  AS YNQGMGV
Sbjct: 181 KKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGV 240

Query: 258 ATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIA 317
           A+GMRFTSRI AYKVCWP GCANADILAA+D AV DGVDVLSLSLGGG+S+FY+D+IAIA
Sbjct: 241 ASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIA 300

Query: 318 TFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSS 377
            FGA++ GVFVSCSAGNSGP  STVGN APWIMT+AASYTDR FP  VKLGNGQV EGSS
Sbjct: 301 AFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSS 360

Query: 378 LYSGKNINQLPLVYNNTAG-GQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKL 437
           LY GK+IN+LPLVYNNTAG GQE NVC AGSL PS VKGKIV+CERGT SRT KGEQVKL
Sbjct: 361 LYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKL 420

Query: 438 AGGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKY 497
           AGG GMILINTQ EGEELFADPHVLPATTLGASAGKAI++YIASSK Q KASI FEGTKY
Sbjct: 421 AGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKY 480

Query: 498 GSRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 557
           GS+APRVAAFSSRGPS+VG DVIKPD+TAPGVNILAAWPPIVSPSEL SD RRV+FNI+S
Sbjct: 481 GSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIIS 540

Query: 558 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 617
           GTSMSCPHVSGLAALLKSAH DWSPAAIKSALMTTAY  D+KMS ISDVG  NG+PA PF
Sbjct: 541 GTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPF 600

Query: 618 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPG 677
            FGSGHVDPE ASDPGLIYDITP+DY+NYLCSL YNS+QIALVSRGN TC SKRT ++PG
Sbjct: 601 TFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPG 660

Query: 678 DLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRL 737
           DLNYPSFS FMKKKAK  ++T KRTVTNVG  RSDYTV+INNPKGVT+IVKPEKLSF  L
Sbjct: 661 DLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSL 720

Query: 738 GEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           GE+LSY+VSFV+LG +EAL +FSFGSLVW+SGKYAVRSPI VTWQ
Sbjct: 721 GEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Spg028522.1 vs. ExPASy TrEMBL
Match: A0A6J1FT86 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111447921 PE=3 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 630/766 (82.25%), Postives = 689/766 (89.95%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATS-TAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLDE 77
           MG  E+WLLLSI+LATS  AA DQQ+YIIHMD TKMATTNP+QWY S+ID++N++SS+++
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 78  ---EASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 137
              EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFL+ATPD+LLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 138 GLQRGHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSN 197
           GLQRGHGLWN+SNL SDIIIGV+DTGIWPEHISF+DKGLP VPKKWKG CQAGP FSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 198 CNKKLVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGM 257
           CNKKL+GA AYIKGYE I+GRLN TGTFRS RDSDGHGTHTASTAAGNIVYKASLYNQGM
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 258 GVATGMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIA 317
           G ATGMRFTSRIAAYKVCWP+GCA+ DILAAIDRAVVDGVDVLSLSLGGG   FY+D+IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 318 IATFGAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEG 377
           IA FGAVRNGVFVSCSAGNSGPF STVGNVAPWIMT+AASYTDR F   VKLGNGQ+ EG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 378 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVK 437
           SSL+SG +I QLPLVYN TAGG+EANVCTAGSLVPS VKGKIVVCERGTNSR  KGEQVK
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 438 LAGGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTK 497
           LAGG GMILINTQLEGEELFAD HVLPA  LGASAGKAIINYIASSK  PKASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGTR 480

Query: 498 YGSRAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIV 557
           YGSRAPR+AAFSSRGPS     VIKPDITAPGVNILAAWPP+VSPSEL+SDKRRVLFNI+
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 558 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANP 617
           SGTSMSCPHVSG+AALLKSAH +WSPAAIKSALMTTAY NDNK S ISDVG  +G PA+P
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPADP 600

Query: 618 FAFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQP 677
           +AFGSGHVDPE A DPGL+YDI P+DYLNYLCSLNY S Q+ LVSRGNF+CPS R  +QP
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQP 660

Query: 678 GDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRR 737
           GDLNYPSFS  MK +AKN  VTFKRTVTNVG+P SDYTV+INNP G+ + VKP+KLSFRR
Sbjct: 661 GDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFRR 720

Query: 738 LGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
            G+KL YQVSFVALGKRE LS+FSFGSLVW+SGKY+VRSPIAV W+
Sbjct: 721 SGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Spg028522.1 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 843.6 bits (2178), Expect = 1.3e-244
Identity = 427/764 (55.89%), Postives = 551/764 (72.12%), Query Frame = 0

Query: 18  MGFREVWLLLSIVLATSTAALDQQTYIIHMDTTKMATTNPEQWYASMIDSVNKISSLDEE 77
           M FR   +   ++   S  +  +QTY+IH       TT+ +    S+ +S+ +  +++++
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENINDD 78

Query: 78  ASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR 137
             +  EI Y+Y+ A+SGF+A L+  +L ++    GF++A PDELL LHTT+S +FLGL+ 
Sbjct: 79  DFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEF 138

Query: 138 GHGLWNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKK 197
           G GLWN ++L SD+IIG++DTGI PEH+SFRD  + PVP +W+G C  G NFS S CNKK
Sbjct: 139 GIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKK 198

Query: 198 LVGARAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVAT 257
           ++GA A+ KGYE+IVG++N T  FRS RD+ GHGTHTASTAAG+IV KA+ + Q  G+A+
Sbjct: 199 IIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLAS 258

Query: 258 GMRFTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATF 317
           GMRFTSRIAAYK CW  GCA+ D++AAIDRA++DGVDV+SLSLGG +  FY D IAIA F
Sbjct: 259 GMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGF 318

Query: 318 GAVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLY 377
           GA++  +FVSCSAGNSGP  STV N APW+MT+AASYTDR FPA+V++GN + L GSSLY
Sbjct: 319 GAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLY 378

Query: 378 SGKNINQLPLVYNNTAGGQEANV-CTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAG 437
            GK++  LPL +N TAG +   V C   SL    V+GKIV+C RG + RTAKGE+VK +G
Sbjct: 379 KGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSG 438

Query: 438 GAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGS 497
           GA M+L++T+ EGEEL ADPHVLPA +LG S GK ++NY+A + +   AS+ F GT YG+
Sbjct: 439 GAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYGA 498

Query: 498 RAPRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSGT 557
            AP VAAFSSRGPS+ G ++ KPDI APG+NILA W P  SPS L SD RRV FNI+SGT
Sbjct: 499 TAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGT 558

Query: 558 SMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGK-PANPFA 617
           SM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA   DN+  PI D G+   +  A  FA
Sbjct: 559 SMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFA 618

Query: 618 FGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGD 677
           FG+G+VDP  A DPGL+YD +  DYLNYLCSLNY S +I L S  N+TC S    L PGD
Sbjct: 619 FGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGD 678

Query: 678 LNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRRLG 737
           LNYPSF+  +   A   TV +KRTVTNVGSP  +Y V +  PKGV + V+P+ L F++  
Sbjct: 679 LNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKAR 738

Query: 738 EKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           E+LSY V++ A   R + S  SFG LVW+  KY VRSPIAVTW+
Sbjct: 739 ERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Spg028522.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 701.4 bits (1809), Expect = 7.9e-202
Identity = 371/753 (49.27%), Postives = 501/753 (66.53%), Query Frame = 0

Query: 34  STAALDQQTYIIHMDTTKMATTNP--EQWYASMIDSVNKISSLDEEASNAAEILYVYKTA 93
           S+++ DQ TYI+HM  ++M ++      WY S + S+          S++AE+LY Y+ A
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI----------SDSAELLYTYENA 82

Query: 94  ISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL-QRGHGLWNSSNLGSD 153
           I GF+ +L+  +  SL   PG ++  P+   +LHTT +P FLGL +    L+  +   SD
Sbjct: 83  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 142

Query: 154 IIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGARAYIKGYEA 213
           +++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG NF+ S CN+KL+GAR + +GYE+
Sbjct: 143 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 202

Query: 214 IVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRFTSRIAAYKV 273
            +G ++ +   RSPRD DGHGTHT+STAAG++V  ASL     G A GM   +R+A YKV
Sbjct: 203 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 262

Query: 274 CWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVRNGVFVSCSA 333
           CW  GC ++DILAAID+A+ D V+VLS+SLGGG S +YRD +AI  F A+  G+ VSCSA
Sbjct: 263 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 322

Query: 334 GNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKNI--NQLPLV 393
           GN+GP +S++ NVAPWI T+ A   DR FPA+  LGNG+   G SL+ G+ +    LP +
Sbjct: 323 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 382

Query: 394 Y-NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQL 453
           Y  N +     N+C  G+L+P KVKGKIV+C+RG N+R  KG+ VK AGG GMIL NT  
Sbjct: 383 YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAA 442

Query: 454 EGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGSR-APRVAAFSS 513
            GEEL AD H+LPATT+G  AG  I +Y+ +  + P ASI+  GT  G + +P VAAFSS
Sbjct: 443 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFSS 502

Query: 514 RGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVSGTSMSCPHVSGL 573
           RGP+ +  +++KPD+ APGVNILAAW     P+ L SD RRV FNI+SGTSMSCPHVSGL
Sbjct: 503 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 562

Query: 574 AALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPFAFGSGHVDPENA 633
           AALLKS H +WSPAAI+SALMTTAY       P+ D+ +  GKP+ PF  G+GHV P  A
Sbjct: 563 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT--GKPSTPFDHGAGHVSPTTA 622

Query: 634 SDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPGDLNYPSFSAFMK 693
           ++PGLIYD+T EDYL +LC+LNY S QI  VSR N+TC   +++    DLNYPSF+  + 
Sbjct: 623 TNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY-SVADLNYPSFAVNVD 682

Query: 694 KKAKNFTVTFKRTVTNVGSPRSDYTVQI-NNPKGVTIIVKPEKLSFRRLGEKLSYQVSFV 753
                    + RTVT+VG     Y+V++ +   GV I V+P  L+F+   EK SY V+F 
Sbjct: 683 GVG---AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT 742

Query: 754 ALGKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
               + + S  SFGS+ W  GK+ V SP+A++W
Sbjct: 743 VDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Spg028522.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 698.0 bits (1800), Expect = 8.8e-201
Identity = 381/768 (49.61%), Postives = 494/768 (64.32%), Query Frame = 0

Query: 32  ATSTAALDQQTYIIHMDTTKMATTNPE--QWYASMIDSVNKISSLDEEASNAAEILYVYK 91
           ++S ++ +  TYI+H+D     +  P    WY S + S+          S+   I++ Y 
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL---------TSSPPSIIHTYD 76

Query: 92  TAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGH--GLWNSSNL 151
           T   GF+A+L+++    L   P  ++  P+++  LHTT SP+FLGL+     GL   S+ 
Sbjct: 77  TVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDF 136

Query: 152 GSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGARAYIKG 211
           GSD++IGV+DTG+WPE  SF D+GL PVP KWKG C A  +F  S CN+KLVGAR +  G
Sbjct: 137 GSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGG 196

Query: 212 YEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRFTSRIAA 271
           YEA  G++N T  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA GM   +R+AA
Sbjct: 197 YEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAA 256

Query: 272 YKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVRNGVFVS 331
           YKVCW  GC ++DILAA D AV DGVDV+SLS+GG    +Y D IAI  FGA+  G+FVS
Sbjct: 257 YKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVS 316

Query: 332 CSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKNIN---Q 391
            SAGN GP   TV NVAPW+ T+ A   DR FPA VKLGNG+++ G S+Y G  ++    
Sbjct: 317 ASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRM 376

Query: 392 LPLVYNNT---AGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAGGAGMI 451
            PLVY  +     G  +++C  GSL P+ VKGKIV+C+RG NSR  KGE V+  GG GMI
Sbjct: 377 YPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMI 436

Query: 452 LINTQLEGEELFADPHVLPATTLGASAGKAIINYIA------SSKHQPKASITFEGTKYG 511
           + N   +GE L AD HVLPAT++GAS G  I  YI+      SSKH P A+I F+GT+ G
Sbjct: 437 IANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKH-PTATIVFKGTRLG 496

Query: 512 SR-APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 571
            R AP VA+FS+RGP+    +++KPD+ APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 497 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 556

Query: 572 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 631
           GTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAYT DN   P+ D   + G  ++  
Sbjct: 557 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 616

Query: 632 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRTFLQPG 691
            +GSGHV P  A DPGL+YDIT  DY+N+LC+ NY  T I  ++R    C   R     G
Sbjct: 617 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 676

Query: 692 DLNYPSFS-AFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFRR 751
           +LNYPSFS  F +      +  F RTVTNVG   S Y ++I  P+G T+ V+PEKLSFRR
Sbjct: 677 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 736

Query: 752 LGEKLSY--QVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTWQ 780
           +G+KLS+  +V    +      +    G +VW  GK  V SP+ VT Q
Sbjct: 737 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Spg028522.1 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 696.8 bits (1797), Expect = 2.0e-200
Identity = 370/766 (48.30%), Postives = 501/766 (65.40%), Query Frame = 0

Query: 26  LLSIVLATSTAALDQQTYIIHMDTTKMAT--TNPEQWYASMIDSVNKISSLDEEASNAAE 85
           L+ +   T+T    ++TY+IHMD + M    TN  QWY+S I+SV +  S +EE +N   
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNN-NR 79

Query: 86  ILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR--GHGL 145
           ILY Y+TA  G AA+L+  +   L +  G +A  P+   +LHTT SP FLGL+R     +
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 146 WNSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGA 205
           W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G  F + NCN+K+VGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 206 RAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMRF 265
           R + +GYEA  G+++    ++SPRD DGHGTHTA+T AG+ V  A+L+    G A GM  
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 266 TSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLGGGASAFYRDDIAIATFGAVR 325
            +R+AAYKVCW  GC ++DIL+A+D+AV DGV VLS+SLGGG S + RD ++IATFGA+ 
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAME 319

Query: 326 NGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYSGKN 385
            GVFVSCSAGN GP   ++ NV+PWI T+ AS  DR FPA VK+G  +  +G SLY G+ 
Sbjct: 320 MGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRT 379

Query: 386 I----NQLPLVY--NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLA 445
           +     Q PLVY   N +     + C  G+L    V GKIV+C+RG   R  KG+ VK A
Sbjct: 380 VLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRA 439

Query: 446 GGAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYG 505
           GG GM+L NT   GEEL AD H+LPA  +G   GK I  Y  +SK +  AS+   GT+ G
Sbjct: 440 GGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGTRIG 499

Query: 506 SR-APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 565
            + +P VAAFSSRGP+ +  +++KPD+ APGVNILAAW   ++PS L SD RRV FNI+S
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 566 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 625
           GTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY +DN   P++D  ++   P++P+
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD--ASGAAPSSPY 619

Query: 626 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSR-GNFTCPSKRTFLQ- 685
             G+GH+DP  A+DPGL+YDI P++Y  +LC+ + + +Q+ + ++  N TC  K T  + 
Sbjct: 620 DHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAKN 679

Query: 686 PGDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGSPRSDYTVQINNPKGVTIIVKPEKLSFR 745
           PG+LNYP+ SA   +      +T +RTVTNVG   S Y V ++  KG ++ V+P+ L+F 
Sbjct: 680 PGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFT 739

Query: 746 RLGEKLSYQVSFVALGKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
              +KLSY V+F     R  +    FG LVW S  + VRSP+ +TW
Sbjct: 740 SKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Spg028522.1 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 676.8 bits (1745), Expect = 2.1e-194
Identity = 369/770 (47.92%), Postives = 502/770 (65.19%), Query Frame = 0

Query: 25  LLLSIVLATSTAALDQQTYIIHMDTTKMAT--TNPEQWYASMIDSVNKISSLDEEASNAA 84
           LLL     +S+++   ++YI+H+  +   +  ++   W+ S++ S+         +   A
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL-------PSSPQPA 74

Query: 85  EILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGHGLW 144
            +LY Y  A+ GF+A+LS  +  +L + P  ++  PD+  ++HTTH+P FLG  +  GLW
Sbjct: 75  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLW 134

Query: 145 NSSNLGSDIIIGVLDTGIWPEHISFRDKGLPPVPKKWKGICQAGPNFSRSNCNKKLVGAR 204
           ++SN G D+I+GVLDTGIWPEH SF D GL P+P  WKG C+ GP+F  S+CN+KL+GAR
Sbjct: 135 SNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGAR 194

Query: 205 AYIKGY--EAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVYKASLYNQGMGVATGMR 264
           A+ +GY  +    + +A    RSPRD++GHGTHTASTAAG++V  ASLY    G ATGM 
Sbjct: 195 AFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMA 254

Query: 265 FTSRIAAYKVCWPQGCANADILAAIDRAVVDGVDVLSLSLG--GGASAFYRDDIAIATFG 324
             +RIAAYK+CW  GC ++DILAA+D+AV DGV V+SLS+G  G A  ++ D IAI  FG
Sbjct: 255 SKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFG 314

Query: 325 AVRNGVFVSCSAGNSGPFTSTVGNVAPWIMTIAASYTDRAFPAMVKLGNGQVLEGSSLYS 384
           A R+G+ VSCSAGNSGP   T  N+APWI+T+ AS  DR F A    G+G+V  G+SLY+
Sbjct: 315 ATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYA 374

Query: 385 GKNI--NQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTAKGEQVKLAG 444
           G+++  +QL LVY+   G   + +C  G L  S V+GKIV+C+RG N+R  KG  VKLAG
Sbjct: 375 GESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAG 434

Query: 445 GAGMILINTQLEGEELFADPHVLPATTLGASAGKAIINYIASSKHQPKASITFEGTKYGS 504
           GAGMIL NT   GEEL AD H++PAT +GA AG  I +YI +S   P A I+F GT  G 
Sbjct: 435 GAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIGP 494

Query: 505 R--APRVAAFSSRGPSIVGADVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIVS 564
              +PRVAAFSSRGP+ +   ++KPD+ APGVNILA W  +V P++L+ D RRV FNI+S
Sbjct: 495 SPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIIS 554

Query: 565 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSTNGKPANPF 624
           GTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N   PI D+ +  GK +N F
Sbjct: 555 GTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLAT--GKSSNSF 614

Query: 625 AFGSGHVDPENASDPGLIYDITPEDYLNYLCSLNYNSTQIALVSRGNFTCPSKRT--FLQ 684
             G+GHVDP  A +PGL+YDI  ++Y+ +LC++ Y    I +  +      +  T     
Sbjct: 615 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRT 674

Query: 685 PGDLNYPSFSAFMKKKAKNFTVTFKRTVTNVGS-PRSDYTVQINNPKGVTIIVKPEKLSF 744
            GDLNYPSFS       +   V +KR V NVGS   + Y V + +P  V I V P KL+F
Sbjct: 675 AGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAF 734

Query: 745 RRLGEKLSYQVSFVAL---GKREALSEFSFGSLVWLSGKYAVRSPIAVTW 779
            +    L Y+V+F ++   G   ++    FGS+ W  G++ V+SP+AV W
Sbjct: 735 SKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769