Spg027984.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg027984.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectinesterase
Location	scaffold2: 46148720 .. 46150927 (-)
Sequence length	1749
RNA-Seq Expression	Spg027984.1
Synteny	Spg027984.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGAGAGAGAAAAAAAACCCTTTTGGTCATTCAAAAAAAAAAAAAAAGAAAAAGAAAAAAAGAGGTTTTTTTTTAGGACAAAATGAGTTATAGGGATGATGATGCTCAGAACAAGAAAAGATACGCCATCATCGGCGTGTCGTCGATGTTACTCGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAGGACGAAACGACCGATCCGGCAACGGGAAACAAGTCCCATGAAATATCTTCTTCTGTGAAACTGATTAAAGACATTTGCCAACCTACTGATTACAAACAAGAATGTGTTGAAAGCCTCAAGTCTGCTGGCACTTCGGATCCCAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCGTGAAATTGATCCAAGCCGCTGCTAATAAATCTATCACCTTGAAAGAGATTGAGAAAGACCCTAGAGCCTCCAAAGCCCTCGCTGGTTGCAAGGAGCTCATGGATTTCGCCATCGAGGAGCTTAAATGGTCGATCAAGAGACTCGGCGAGTTTGATATTACCAAACTCGACGAGATGTTGATTGACATTAAGATCTGGCTCAGCGCCACCATTACCTATCAGGTAACAAAAGTTTAAGCTGATGAGTTAAAAGTATTTAAAGTGAATAGATTAATTTAATTGGTTTGATGTTGATTGATGTAGGAAACTTGCTTGGATGGGTTCGAGAACACGACGGGAAATGCGGCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGAGAGTGAGCAGCAATGGGTTGGCGATGGTGACGCAAATCTCGTCGATGTTGACGGATTTGCAAATTCCGGGGATCAACCGACGAAGACTTCTGGATATTCCAGTTCTTGGGCACGACGATTACCCCGACTGGGCGAACCCCGGGATGCGGCGACTCCTTGCGGCGAAGGGGAAGCCCAACATCGTGGTGGCCAAGGATGGAAGTGGGCAGTACAAGACCATTCAAGAGGCTCTGAACAGAGTCCCAAAGAGGAAGAACAACCAAACATTTGTGATTCACATCAAGCAAGGAGTGTACAAGGAATACCTGACCATCAACAAAACCCTCACCCATTTGATGCTCATTGGCGATGGCCCCAAAAAGACCATCATCTCTGGAAACAAGAACTTTATTGATGGCACTCCCACCTTCAAAACTGCAACTGTTGCTGTGACAGCGGAGCACTTCATGGCCAGAGACATCGGGTTCGAGAACACGGCAGGCCCAGAGAAGCACCAAGCAGTGGCATTGCGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAGATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGCCAATTCTACCGGGACTGCGTCGTCTCCGGCACCATCGACTTCGTCTTCGGGGACGCGGCCGCCGTCTTCCAAGGCTGCACCTTCCTGGTGCGCAAGCCCCTTCCAAACCAACAATGCATCGTCACGGCCCACGGGCGCAAGGAGAGGCGGCAGCCCTCCGCCCTCATCATCCAGAACTCCTCCTTCAAGCCCGACACCGACCTCGTCCCGGTGAAAAAGCAGTTCCGATCCTACCTCGGACGACCCTGGAAGGAGTACTCCAGAACCATCATAATGGAATCATTCATCGACGACTTGATCCAGCCAGAAGGGTGGCTCCCATGGGCAGGGGACTGGGGCCTCAAGACTTGCTTCTACACAGAGTTCAACTGTTATGGCCCAGGCTCCGACAAGTCCAAGAGGGTCAAGTGGAGGGGGATCAAGACCATTACGCCAAAGCATGCCCTCGATTTCACTGTAGGCAGGTTCATTTTGGGGGATAGGTGGATTAAGCCCACTGGAGTTCCATATGTCTCCGGCATGACCAAAGCCGGCGGCGCCGCCACCAAGGGTGCAGCTGCTCATTGATTTTAGTGGTTCTCTTCATCATTTATTCTTATGTGTAATGGCGCCCGGCCACCCGCTTGGCTGGCTCTTTTTTCTTCTTCTTTTTCTGTCTCTCTCTAATGTGGTTTAAGTTGTGTGTGTTCATGTGTGGGTCTAATTCTATATGGTGGAAGAAAATATTTTTTCTTTTTTCCTTTTTTTTTTCCCAAATGTTTCATTTCTTTGCATTTCTTTCATTTTTTAGAGTTTGGATTTGACCCCCTTTATTATTTCATTCTTTTATTAATAAATCCCAATTAATGGCTTCATTATTAA

mRNA sequence

ATGAGTTATAGGGATGATGATGCTCAGAACAAGAAAAGATACGCCATCATCGGCGTGTCGTCGATGTTACTCGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAGGACGAAACGACCGATCCGGCAACGGGAAACAAGTCCCATGAAATATCTTCTTCTGTGAAACTGATTAAAGACATTTGCCAACCTACTGATTACAAACAAGAATGTGTTGAAAGCCTCAAGTCTGCTGGCACTTCGGATCCCAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCGTGAAATTGATCCAAGCCGCTGCTAATAAATCTATCACCTTGAAAGAGATTGAGAAAGACCCTAGAGCCTCCAAAGCCCTCGCTGGTTGCAAGGAGCTCATGGATTTCGCCATCGAGGAGCTTAAATGGTCGATCAAGAGACTCGGCGAGTTTGATATTACCAAACTCGACGAGATGTTGATTGACATTAAGATCTGGCTCAGCGCCACCATTACCTATCAGGAAACTTGCTTGGATGGGTTCGAGAACACGACGGGAAATGCGGCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGAGAGTGAGCAGCAATGGGTTGGCGATGGTGACGCAAATCTCGTCGATGTTGACGGATTTGCAAATTCCGGGGATCAACCGACGAAGACTTCTGGATATTCCAGTTCTTGGGCACGACGATTACCCCGACTGGGCGAACCCCGGGATGCGGCGACTCCTTGCGGCGAAGGGGAAGCCCAACATCGTGGTGGCCAAGGATGGAAGTGGGCAGTACAAGACCATTCAAGAGGCTCTGAACAGAGTCCCAAAGAGGAAGAACAACCAAACATTTGTGATTCACATCAAGCAAGGAGTGTACAAGGAATACCTGACCATCAACAAAACCCTCACCCATTTGATGCTCATTGGCGATGGCCCCAAAAAGACCATCATCTCTGGAAACAAGAACTTTATTGATGGCACTCCCACCTTCAAAACTGCAACTGTTGCTGTGACAGCGGAGCACTTCATGGCCAGAGACATCGGGTTCGAGAACACGGCAGGCCCAGAGAAGCACCAAGCAGTGGCATTGCGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAGATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGCCAATTCTACCGGGACTGCGTCGTCTCCGGCACCATCGACTTCGTCTTCGGGGACGCGGCCGCCGTCTTCCAAGGCTGCACCTTCCTGGTGCGCAAGCCCCTTCCAAACCAACAATGCATCGTCACGGCCCACGGGCGCAAGGAGAGGCGGCAGCCCTCCGCCCTCATCATCCAGAACTCCTCCTTCAAGCCCGACACCGACCTCGTCCCGGTGAAAAAGCAGTTCCGATCCTACCTCGGACGACCCTGGAAGGAGTACTCCAGAACCATCATAATGGAATCATTCATCGACGACTTGATCCAGCCAGAAGGGTGGCTCCCATGGGCAGGGGACTGGGGCCTCAAGACTTGCTTCTACACAGAGTTCAACTGTTATGGCCCAGGCTCCGACAAGTCCAAGAGGGTCAAGTGGAGGGGGATCAAGACCATTACGCCAAAGCATGCCCTCGATTTCACTGTAGGCAGGTTCATTTTGGGGGATAGGTGGATTAAGCCCACTGGAGTTCCATATGTCTCCGGCATGACCAAAGCCGGCGGCGCCGCCACCAAGGGTGCAGCTGCTCATTGA

Coding sequence (CDS)

ATGAGTTATAGGGATGATGATGCTCAGAACAAGAAAAGATACGCCATCATCGGCGTGTCGTCGATGTTACTCGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAGGACGAAACGACCGATCCGGCAACGGGAAACAAGTCCCATGAAATATCTTCTTCTGTGAAACTGATTAAAGACATTTGCCAACCTACTGATTACAAACAAGAATGTGTTGAAAGCCTCAAGTCTGCTGGCACTTCGGATCCCAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCGTGAAATTGATCCAAGCCGCTGCTAATAAATCTATCACCTTGAAAGAGATTGAGAAAGACCCTAGAGCCTCCAAAGCCCTCGCTGGTTGCAAGGAGCTCATGGATTTCGCCATCGAGGAGCTTAAATGGTCGATCAAGAGACTCGGCGAGTTTGATATTACCAAACTCGACGAGATGTTGATTGACATTAAGATCTGGCTCAGCGCCACCATTACCTATCAGGAAACTTGCTTGGATGGGTTCGAGAACACGACGGGAAATGCGGCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGAGAGTGAGCAGCAATGGGTTGGCGATGGTGACGCAAATCTCGTCGATGTTGACGGATTTGCAAATTCCGGGGATCAACCGACGAAGACTTCTGGATATTCCAGTTCTTGGGCACGACGATTACCCCGACTGGGCGAACCCCGGGATGCGGCGACTCCTTGCGGCGAAGGGGAAGCCCAACATCGTGGTGGCCAAGGATGGAAGTGGGCAGTACAAGACCATTCAAGAGGCTCTGAACAGAGTCCCAAAGAGGAAGAACAACCAAACATTTGTGATTCACATCAAGCAAGGAGTGTACAAGGAATACCTGACCATCAACAAAACCCTCACCCATTTGATGCTCATTGGCGATGGCCCCAAAAAGACCATCATCTCTGGAAACAAGAACTTTATTGATGGCACTCCCACCTTCAAAACTGCAACTGTTGCTGTGACAGCGGAGCACTTCATGGCCAGAGACATCGGGTTCGAGAACACGGCAGGCCCAGAGAAGCACCAAGCAGTGGCATTGCGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAGATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGCCAATTCTACCGGGACTGCGTCGTCTCCGGCACCATCGACTTCGTCTTCGGGGACGCGGCCGCCGTCTTCCAAGGCTGCACCTTCCTGGTGCGCAAGCCCCTTCCAAACCAACAATGCATCGTCACGGCCCACGGGCGCAAGGAGAGGCGGCAGCCCTCCGCCCTCATCATCCAGAACTCCTCCTTCAAGCCCGACACCGACCTCGTCCCGGTGAAAAAGCAGTTCCGATCCTACCTCGGACGACCCTGGAAGGAGTACTCCAGAACCATCATAATGGAATCATTCATCGACGACTTGATCCAGCCAGAAGGGTGGCTCCCATGGGCAGGGGACTGGGGCCTCAAGACTTGCTTCTACACAGAGTTCAACTGTTATGGCCCAGGCTCCGACAAGTCCAAGAGGGTCAAGTGGAGGGGGATCAAGACCATTACGCCAAAGCATGCCCTCGATTTCACTGTAGGCAGGTTCATTTTGGGGGATAGGTGGATTAAGCCCACTGGAGTTCCATATGTCTCCGGCATGACCAAAGCCGGCGGCGCCGCCACCAAGGGTGCAGCTGCTCATTGA

Protein sequence

MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIKDICQPTDYKQECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPDWANPGMRRLLAAKGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH

Homology

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BLAST of Spg027984.1 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.8e-301
Identity = 513/586 (87.54%), Postives = 552/586 (94.20%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           MSY DDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ET+DPA G+KSHEIS+SVK 
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQECV SLK+AG  +SDPKELVQAGFKAA+KLIQAAANKS+TLKEIEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA++ELK+S+ +LGEFDI+KLDEMLIDIKIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
           ENTTGNAAEKMKKALKTSM++SSNGLAMV+QISSMLT+LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  + KPN+VVAKDGSGQ++TIQEAL++VPKRKNN T+VIHIKQGVY
Sbjct: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           KEYL I K+LTHLMLIGDGPKKTII+G+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC +SGTIDFVFGDAAAV
Sbjct: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFK D DL PV+KQFRS+LGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGLKTCFYTE+N YGPGSDKSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HA+DFT GRF+ GD WIK TGVPYVSGMT+   AA +G A H
Sbjct: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH 586

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BLAST of Spg027984.1 vs. NCBI nr
Match: XP_008450602.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >KAA0050920.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa] >TYK10268.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 1042.0 bits (2693), Expect = 2.0e-300
Identity = 507/586 (86.52%), Postives = 554/586 (94.54%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M Y+DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDET+DPATGNKSHEISSS+K 
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQEC+ SLK+ G  +SDP+ELVQAGFKAA+KLIQAAANKS+ LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAI+ELK+S+ +LGEFDITKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM++SSNGLAMV+Q SSMLT+LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  + KPN+VVAKDGSGQ++TIQ AL++VPKRKNN T+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL I KT+THLMLIGDGPKKTII+GNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP+KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC VSGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S K D DL PV+KQFRS+LGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGLKTCFYTE+N YGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HALDFT GRF++GDRWIKPTGVPYVSGMT+ GGAA   AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA---AAAH 583

[Close]

BLAST of Spg027984.1 vs. NCBI nr
Match: XP_004135775.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.1 hypothetical protein Csa_007353 [Cucumis sativus])

HSP 1 Score: 1041.6 bits (2692), Expect = 2.6e-300
Identity = 508/586 (86.69%), Postives = 554/586 (94.54%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M Y+DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDET+DPATGNKSHEISSS+K 
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQECV SLK+ G  +SDPKELVQAGFKAA+KLIQAAANKS+ L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAI+ELK+S+ +LGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
            NTTGNAAEKMKKALKTSM++SSNGLAMV+QISSML++LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  K KPN+VVAKDGSGQ+KTIQEA+++VPKRKNN T+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+ + KTLTHLMLIGDGPKKTII+GNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP+KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC VSGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFKP  DLVPV+KQFRS+LGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGL+TCFYTE+N YGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HA+DFT GRF+ GDRWIKPTGVPYVSG+T+ GGA   GAAAH
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGA---GAAAH 583

[Close]

BLAST of Spg027984.1 vs. NCBI nr
Match: XP_022969472.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 995.0 bits (2571), Expect = 2.8e-286
Identity = 485/573 (84.64%), Postives = 533/573 (93.02%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD T+DPATG K+HEISSS+K 
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 120
           IK IC+PTDYK+ECV+SLK +G SDPKELVQ GFKAA+KLIQAAA KS+TL++IEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPRA 120

Query: 121 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 180
           S+ALAGCKELMDF+++ELK+SI++LG FDI+KLDEMLIDIKIWLSA ITYQETCLDGF N
Sbjct: 121 SQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 240
           +TG+A+EKMKKALK SMR+SSNGLAMVT++SSMLT+LQIPGIN RRLL IPVLGH+DYP+
Sbjct: 181 STGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYPE 240

Query: 241 WANPGMRRLLA---AKGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 300
           WA PGM+RLLA   AK KPNIVVAKDGSGQYKTIQEA+N+VPKRKNN TFVIHIKQGVYK
Sbjct: 241 WAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 300

Query: 301 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 360
           EY+ I K LTHLMLIGDGPKKTIISGNKNF+DGT TFKTATVAV+AEHFMARDIGFENTA
Sbjct: 301 EYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENTA 360

Query: 361 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 420
           GP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDC +SGTIDFVFGDAAAVF
Sbjct: 361 GPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVF 420

Query: 421 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 480
           Q C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FKPD DLVPVKKQFRSYLGRPW
Sbjct: 421 QNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPW 480

Query: 481 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 540
           KEYSRTIIMES+IDD+I+PEGWLPW GDWGL+TCFYTEFN YGPGS KSKRVKWRGIK+I
Sbjct: 481 KEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKSI 540

Query: 541 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTK 571
           T +HA+DFT GRFILGDRWIK TG+PYVSGMTK
Sbjct: 541 TAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

[Close]

BLAST of Spg027984.1 vs. NCBI nr
Match: XP_022931680.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 991.9 bits (2563), Expect = 2.4e-285
Identity = 483/573 (84.29%), Postives = 534/573 (93.19%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD T+DPATG K+HEISSS+K 
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 120
           IK IC+PTDYK+ECV+SLK +G SDPKELVQ GFKAA+KLIQAAA KS+TL++IEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA 120

Query: 121 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 180
           S+ALA CKELMD++++ELK+SI++LG FDI+KLDEMLIDIKIWLSA ITYQETCLDGF N
Sbjct: 121 SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 240
           TTG+A+EKMKKALKTSMR+SSNGLAMVT++SSMLT+LQIPGIN RRLL IPVLGH+D+P+
Sbjct: 181 TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFPE 240

Query: 241 WANPGMRRLLA---AKGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 300
           W +PGM+RLLA   AK KPNIVVAKDGSGQYKTIQEA+N+VPKRKNN TFVIHIKQGVYK
Sbjct: 241 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 300

Query: 301 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 360
           EY+ I K LTHLMLIGDGPKKTII+GNKNF+DGT TFKTATVAV+AEHFMARDIGFENTA
Sbjct: 301 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTA 360

Query: 361 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 420
           GP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDC +SGTIDFVFGDAAAVF
Sbjct: 361 GPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVF 420

Query: 421 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 480
           Q C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FKPD DLVPVKKQFRSYLGRPW
Sbjct: 421 QNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPW 480

Query: 481 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 540
           KEYSRTIIMES+IDD+I PEGWLPWAGDWGL+TCFYTEFN +GPGS K+KRVKWRGIK+I
Sbjct: 481 KEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSI 540

Query: 541 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTK 571
           TP+HA+DFT GRFILGDRWIK TGVPYVSGMTK
Sbjct: 541 TPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572

[Close]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 3.1e-187
Identity = 319/577 (55.29%), Postives = 428/577 (74.18%), Query Frame = 0


Query: 3   YRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIK 62
           Y D+DA+ KKRY II +SS+LL++MVVAVT+GV++N+ +           EI++SVK IK
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAIK 64

Query: 63  DICQPTDYKQECVESLK--SAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 122
           D+C PTDYK+ C ++L+  +  TSDP ELV+  F A +K I   A KS T+ E++KDPRA
Sbjct: 65  DVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRA 124

Query: 123 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 182
             AL  CKELMD+AI EL  S + LG+F+  K+DE L+ ++IWLSATI++++TCLDGF+ 
Sbjct: 125 KMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQG 184

Query: 183 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 242
           T GNA E +KKALKT+++++ NGLAMVT++S+ L  +QIP +N RRLL        ++P 
Sbjct: 185 TQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFPS 244

Query: 243 WANPGMRRLLAA---KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 302
           W +   RRLL A   + KP+IVVA+DGSGQYKTI EALN VPK+KN  TFV+HIK+G+YK
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIYK 304

Query: 303 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 362
           EY+ +N+++THL+ IGDGP KT+ISG+K++ DG  T+KTATVA+  +HF+A++I FENTA
Sbjct: 305 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 364

Query: 363 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 422
           G  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDC +SGTIDF+FGDAAAVF
Sbjct: 365 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 424

Query: 423 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 482
           Q CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q  +   + D + VK+Q ++YLGRPW
Sbjct: 425 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 484

Query: 483 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 542
           KEYSRTIIM +FI D + PEGW PW G++GL T FY+E    GPG+  +KRV W GIK +
Sbjct: 485 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 544

Query: 543 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGA 575
           + +  L FT  ++I GD WI   GVPY+ G+    G+
Sbjct: 545 SDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGS 568

[Close]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 642.1 bits (1655), Expect = 6.0e-183
Identity = 315/583 (54.03%), Postives = 424/583 (72.73%), Query Frame = 0


Query: 1   MSY-RDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVK 60
           MSY  DD+++ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E+++SVK
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD----GDSKGKAEVNASVK 60

Query: 61  LIKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKD 120
            +KD+C PTDY++ C ++L   G  T+DP ELV+  F   +K I  AA KS T+ E++KD
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDG 180
            R   AL  CKELMD+A++EL  S + LG+F+   LDE LI+++IWLSA I+++ETCL+G
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDD 240
           F+ T GNA E MKKALKT++ ++ NGLA+++++S+ +  +QIPG+N RRLL       + 
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLL------AEG 240

Query: 241 YPDWANPGMRRLLAAKG-----KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIK 300
           +P W +   R+LL A       KP+IVVA+DGSGQYKTI EAL  VPK K N TFV+HIK
Sbjct: 241 FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIK 300

Query: 301 QGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIG 360
            G+YKEY+ +NKT++HL+ IGDGP KTIISGNKN+ DG  T++TATVA+   +F+A++IG
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 FENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGD 420
           FENTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDC +SGTIDF+FGD
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSY 480
           AAAVFQ CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q  +   + D + VK+  ++Y
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWR 540
           LGRPWKEYSRTIIM +FI D +QP+GW PW GD+GLKT FY+E    GPGS  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKTITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAA 576
           GIKT++ +  L FT  ++I GD WI   GVPY +G+     AA
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAA 572

[Close]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.4e-149
Identity = 271/575 (47.13%), Postives = 374/575 (65.04%), Query Frame = 0


Query: 6   DDAQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETTDPATGNKSHEISSSVKLIKD 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I ++   ++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANK-SITL-KEIEKDPR 125
           +C PTDYK+ CV SL  A   ++ P +L++ GF   ++ I+ +  K S+ L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 ASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFE 185
              AL  C++LM+ A ++LK  +     F I ++++ + D+++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGIN------RRRLLDIPVL 245
            T    ++ M+K  KTS  ++SNGLAM+T IS++L +  + G+        R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAKG--KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHI 305
             D  P W  P  RRL+A KG  K N+VVA DGSGQYKTI EALN VPK  N + FVI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYI 303

Query: 306 KQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDG-TPTFKTATVAVTAEHFMARD 365
           KQGVY E + + K +TH+  IGDGP KT I+G+ N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVF 425
           IGFENTAGPE HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDC VSGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFR 485
           GD   V Q C  +VRKP+ +Q C++TA GR ++R+ + L++QN     +   +PVK   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SYLGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVK 545
           +YLGRPWKE+SRTIIM + IDD+I P GWLPW GD+ L T +Y E+   GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKTITPKHALDFTVGRFILGDRWIKPTGVPYV 566
           W GIK ++PK AL FT  RF+ G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 499.2 bits (1284), Expect = 6.3e-140
Identity = 259/577 (44.89%), Postives = 365/577 (63.26%), Query Frame = 0


Query: 7   DAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIKDICQ 66
           D   KK++ + G  S  LV MVV+V V        T+  +  +  + I  + K ++ +C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSI--TLKEIEKDPRASK 126
           PTD+K  CV SL  A   + DP +L++ GFK  +K I  +  K+      + +K+P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  AI++LK  +     F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGIN------RRRLLDIPVLGHD 246
            N  + M K  KTS  +SSN LAMVT+IS+++ +  + G+        R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAAKG------KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIH 306
             P W  P  RRL+AA+G      K N VVA+DG+GQ+KTI +ALN VPK  N   F+IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK+G+YKE +T+ K + H+  IGDGP KT+I+G+ NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFV 426
           +IG ENTAGPE  QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDC VSGT+DF+
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQF 486
           FGDA  + Q C  +VRKP   Q C+VTA GR   R+ + L++       D   +P+K   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSYLGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRV 546
           ++YLGRPWKE+SRTIIM++ IDD+I P GWLPW+GD+ LKT +Y E    GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPKHALDFTVGRFILGDRWIKPTGVPYVS 567
           KW GIK +TP+ AL +T  RF+ GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.3e-126
Identity = 264/700 (37.71%), Postives = 373/700 (53.29%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIKDICQPTDYKQ 72
           +Y ++GV++ L++AMV+ V    N     +      ++  ++  +   +  IC  TDYKQ
Sbjct: 3   KYVLLGVTA-LIMAMVICVE--ANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQ 62

Query: 73  ECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKS-ITLK-EIEKDPRASKALAGCKEL 132
           +C  SL +  + DP+ L+++ F  A+  I++  ++  I LK   + D    +AL  C+EL
Sbjct: 63  DCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 122

Query: 133 MDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEKMK 192
           MD AI++L+ +  +   F  T+L + + D+ +WLS +ITYQ+TC+DGFE     AA  M+
Sbjct: 123 MDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMME 182

Query: 193 KALKTSMRVSSNGLAMVTQISSMLTDLQIP-----------GI----------------- 252
           + ++    ++SNGLA+   +  +L   +IP           GI                 
Sbjct: 183 RVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQDS 242

Query: 253 ----------------------------------NRR----------------------- 312
                                             N R                       
Sbjct: 243 PPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAFS 302

Query: 313 -------------------------------------------------------RLLDI 372
                                                                   L D 
Sbjct: 303 GNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKDR 362

Query: 373 PVLGHDDYPDWANPGMRRLLAAKG-----KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQ 432
            +    ++P W  P  RRLLA +      K N+VVAKDGSG+ KTI +AL  VP  KN +
Sbjct: 363 HLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKNTK 422

Query: 433 TFVIHIKQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFI-DGTPTFKTATVAVTAE 492
            FVIHIK+GVYKE + + K + H+M +GDGP KT+I+G+  F+ D   T++TA+VAV  +
Sbjct: 423 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 482

Query: 493 HFMARDIGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSG 552
           +FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C VSG
Sbjct: 483 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 542

Query: 553 TIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVP 565
           TIDFVFGDA AVFQ C F++R+P+ +QQCIVTA GRK+RR+ + ++I NS    D   +P
Sbjct: 543 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 602

[Close]

BLAST of Spg027984.1 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.6e-301
Identity = 507/586 (86.52%), Postives = 554/586 (94.54%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M Y+DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDET+DPATGNKSHEISSS+K 
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQEC+ SLK+ G  +SDP+ELVQAGFKAA+KLIQAAANKS+ LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAI+ELK+S+ +LGEFDITKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM++SSNGLAMV+Q SSMLT+LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  + KPN+VVAKDGSGQ++TIQ AL++VPKRKNN T+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL I KT+THLMLIGDGPKKTII+GNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP+KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC VSGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S K D DL PV+KQFRS+LGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGLKTCFYTE+N YGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HALDFT GRF++GDRWIKPTGVPYVSGMT+ GGAA   AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA---AAAH 583

[Close]

BLAST of Spg027984.1 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.6e-301
Identity = 507/586 (86.52%), Postives = 554/586 (94.54%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M Y+DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDET+DPATGNKSHEISSS+K 
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQEC+ SLK+ G  +SDP+ELVQAGFKAA+KLIQAAANKS+ LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAI+ELK+S+ +LGEFDITKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM++SSNGLAMV+Q SSMLT+LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  + KPN+VVAKDGSGQ++TIQ AL++VPKRKNN T+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL I KT+THLMLIGDGPKKTII+GNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP+KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC VSGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S K D DL PV+KQFRS+LGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGLKTCFYTE+N YGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HALDFT GRF++GDRWIKPTGVPYVSGMT+ GGAA   AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA---AAAH 583

[Close]

BLAST of Spg027984.1 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.3e-300
Identity = 508/586 (86.69%), Postives = 554/586 (94.54%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M Y+DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDET+DPATGNKSHEISSS+K 
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDP 120
           IK ICQPTDYKQECV SLK+ G  +SDPKELVQAGFKAA+KLIQAAANKS+ L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAI+ELK+S+ +LGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDY 240
            NTTGNAAEKMKKALKTSM++SSNGLAMV+QISSML++LQIPGI+RRRLL+IPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAA--KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVY 300
           PDWANPGMRRLLAA  K KPN+VVAKDGSGQ+KTIQEA+++VPKRKNN T+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+ + KTLTHLMLIGDGPKKTII+GNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 420
           AGP+KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC VSGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFKP  DLVPV+KQFRS+LGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMES+I DLIQPEGWLPWAGDWGL+TCFYTE+N YGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAATKGAAAH 583
           ITP+HA+DFT GRF+ GDRWIKPTGVPYVSG+T+ GGA   GAAAH
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGA---GAAAH 583

[Close]

BLAST of Spg027984.1 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 995.0 bits (2571), Expect = 1.3e-286
Identity = 485/573 (84.64%), Postives = 533/573 (93.02%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD T+DPATG K+HEISSS+K 
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 120
           IK IC+PTDYK+ECV+SLK +G SDPKELVQ GFKAA+KLIQAAA KS+TL++IEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPRA 120

Query: 121 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 180
           S+ALAGCKELMDF+++ELK+SI++LG FDI+KLDEMLIDIKIWLSA ITYQETCLDGF N
Sbjct: 121 SQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 240
           +TG+A+EKMKKALK SMR+SSNGLAMVT++SSMLT+LQIPGIN RRLL IPVLGH+DYP+
Sbjct: 181 STGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYPE 240

Query: 241 WANPGMRRLLA---AKGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 300
           WA PGM+RLLA   AK KPNIVVAKDGSGQYKTIQEA+N+VPKRKNN TFVIHIKQGVYK
Sbjct: 241 WAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 300

Query: 301 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 360
           EY+ I K LTHLMLIGDGPKKTIISGNKNF+DGT TFKTATVAV+AEHFMARDIGFENTA
Sbjct: 301 EYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENTA 360

Query: 361 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 420
           GP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDC +SGTIDFVFGDAAAVF
Sbjct: 361 GPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVF 420

Query: 421 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 480
           Q C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FKPD DLVPVKKQFRSYLGRPW
Sbjct: 421 QNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPW 480

Query: 481 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 540
           KEYSRTIIMES+IDD+I+PEGWLPW GDWGL+TCFYTEFN YGPGS KSKRVKWRGIK+I
Sbjct: 481 KEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKSI 540

Query: 541 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTK 571
           T +HA+DFT GRFILGDRWIK TG+PYVSGMTK
Sbjct: 541 TAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

[Close]

BLAST of Spg027984.1 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.1e-285
Identity = 483/573 (84.29%), Postives = 534/573 (93.19%), Query Frame = 0


Query: 1   MSYRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKL 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD T+DPATG K+HEISSS+K 
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKDICQPTDYKQECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 120
           IK IC+PTDYK+ECV+SLK +G SDPKELVQ GFKAA+KLIQAAA KS+TL++IEKDPRA
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDPRA 120

Query: 121 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 180
           S+ALA CKELMD++++ELK+SI++LG FDI+KLDEMLIDIKIWLSA ITYQETCLDGF N
Sbjct: 121 SQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFAN 180

Query: 181 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 240
           TTG+A+EKMKKALKTSMR+SSNGLAMVT++SSMLT+LQIPGIN RRLL IPVLGH+D+P+
Sbjct: 181 TTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDFPE 240

Query: 241 WANPGMRRLLA---AKGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 300
           W +PGM+RLLA   AK KPNIVVAKDGSGQYKTIQEA+N+VPKRKNN TFVIHIKQGVYK
Sbjct: 241 WVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 300

Query: 301 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 360
           EY+ I K LTHLMLIGDGPKKTII+GNKNF+DGT TFKTATVAV+AEHFMARDIGFENTA
Sbjct: 301 EYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFENTA 360

Query: 361 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 420
           GP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDC +SGTIDFVFGDAAAVF
Sbjct: 361 GPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVF 420

Query: 421 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 480
           Q C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FKPD DLVPVKKQFRSYLGRPW
Sbjct: 421 QNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPW 480

Query: 481 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 540
           KEYSRTIIMES+IDD+I PEGWLPWAGDWGL+TCFYTEFN +GPGS K+KRVKWRGIK+I
Sbjct: 481 KEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIKSI 540

Query: 541 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTK 571
           TP+HA+DFT GRFILGDRWIK TGVPYVSGMTK
Sbjct: 541 TPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572

[Close]

BLAST of Spg027984.1 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-188
Identity = 319/577 (55.29%), Postives = 428/577 (74.18%), Query Frame = 0


Query: 3   YRDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIK 62
           Y D+DA+ KKRY II +SS+LL++MVVAVT+GV++N+ +           EI++SVK IK
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAIK 64

Query: 63  DICQPTDYKQECVESLK--SAGTSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKDPRA 122
           D+C PTDYK+ C ++L+  +  TSDP ELV+  F A +K I   A KS T+ E++KDPRA
Sbjct: 65  DVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRA 124

Query: 123 SKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFEN 182
             AL  CKELMD+AI EL  S + LG+F+  K+DE L+ ++IWLSATI++++TCLDGF+ 
Sbjct: 125 KMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQG 184

Query: 183 TTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDDYPD 242
           T GNA E +KKALKT+++++ NGLAMVT++S+ L  +QIP +N RRLL        ++P 
Sbjct: 185 TQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFPS 244

Query: 243 WANPGMRRLLAA---KGKPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIKQGVYK 302
           W +   RRLL A   + KP+IVVA+DGSGQYKTI EALN VPK+KN  TFV+HIK+G+YK
Sbjct: 245 WMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIYK 304

Query: 303 EYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTA 362
           EY+ +N+++THL+ IGDGP KT+ISG+K++ DG  T+KTATVA+  +HF+A++I FENTA
Sbjct: 305 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 364

Query: 363 GPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVF 422
           G  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDC +SGTIDF+FGDAAAVF
Sbjct: 365 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 424

Query: 423 QGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSYLGRPW 482
           Q CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q  +   + D + VK+Q ++YLGRPW
Sbjct: 425 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 484

Query: 483 KEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWRGIKTI 542
           KEYSRTIIM +FI D + PEGW PW G++GL T FY+E    GPG+  +KRV W GIK +
Sbjct: 485 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 544

Query: 543 TPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGA 575
           + +  L FT  ++I GD WI   GVPY+ G+    G+
Sbjct: 545 SDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGS 568

[Close]

BLAST of Spg027984.1 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 642.1 bits (1655), Expect = 4.3e-184
Identity = 315/583 (54.03%), Postives = 424/583 (72.73%), Query Frame = 0


Query: 1   MSY-RDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVK 60
           MSY  DD+++ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E+++SVK
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD----GDSKGKAEVNASVK 60

Query: 61  LIKDICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSITLKEIEKD 120
            +KD+C PTDY++ C ++L   G  T+DP ELV+  F   +K I  AA KS T+ E++KD
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDG 180
            R   AL  CKELMD+A++EL  S + LG+F+   LDE LI+++IWLSA I+++ETCL+G
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGINRRRLLDIPVLGHDD 240
           F+ T GNA E MKKALKT++ ++ NGLA+++++S+ +  +QIPG+N RRLL       + 
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLL------AEG 240

Query: 241 YPDWANPGMRRLLAAKG-----KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHIK 300
           +P W +   R+LL A       KP+IVVA+DGSGQYKTI EAL  VPK K N TFV+HIK
Sbjct: 241 FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIK 300

Query: 301 QGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDGTPTFKTATVAVTAEHFMARDIG 360
            G+YKEY+ +NKT++HL+ IGDGP KTIISGNKN+ DG  T++TATVA+   +F+A++IG
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 FENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVFGD 420
           FENTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDC +SGTIDF+FGD
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFRSY 480
           AAAVFQ CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q  +   + D + VK+  ++Y
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVKWR 540
           LGRPWKEYSRTIIM +FI D +QP+GW PW GD+GLKT FY+E    GPGS  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKTITPKHALDFTVGRFILGDRWIKPTGVPYVSGMTKAGGAA 576
           GIKT++ +  L FT  ++I GD WI   GVPY +G+     AA
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAA 572

[Close]

BLAST of Spg027984.1 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 528.9 bits (1361), Expect = 5.3e-150
Identity = 271/575 (47.13%), Postives = 374/575 (65.04%), Query Frame = 0


Query: 6   DDAQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETTDPATGNKSHEISSSVKLIKD 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I ++   ++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANK-SITL-KEIEKDPR 125
           +C PTDYK+ CV SL  A   ++ P +L++ GF   ++ I+ +  K S+ L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 ASKALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFE 185
              AL  C++LM+ A ++LK  +     F I ++++ + D+++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGIN------RRRLLDIPVL 245
            T    ++ M+K  KTS  ++SNGLAM+T IS++L +  + G+        R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAKG--KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIHI 305
             D  P W  P  RRL+A KG  K N+VVA DGSGQYKTI EALN VPK  N + FVI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYI 303

Query: 306 KQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFIDG-TPTFKTATVAVTAEHFMARD 365
           KQGVY E + + K +TH+  IGDGP KT I+G+ N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFVF 425
           IGFENTAGPE HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDC VSGT+DF+F
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQFR 485
           GD   V Q C  +VRKP+ +Q C++TA GR ++R+ + L++QN     +   +PVK   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SYLGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRVK 545
           +YLGRPWKE+SRTIIM + IDD+I P GWLPW GD+ L T +Y E+   GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKTITPKHALDFTVGRFILGDRWIKPTGVPYV 566
           W GIK ++PK AL FT  RF+ G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of Spg027984.1 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 499.2 bits (1284), Expect = 4.5e-141
Identity = 259/577 (44.89%), Postives = 365/577 (63.26%), Query Frame = 0


Query: 7   DAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIKDICQ 66
           D   KK++ + G  S  LV MVV+V V        T+  +  +  + I  + K ++ +C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVESLKSAG--TSDPKELVQAGFKAAVKLIQAAANKSI--TLKEIEKDPRASK 126
           PTD+K  CV SL  A   + DP +L++ GFK  +K I  +  K+      + +K+P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  AI++LK  +     F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMRVSSNGLAMVTQISSMLTDLQIPGIN------RRRLLDIPVLGHD 246
            N  + M K  KTS  +SSN LAMVT+IS+++ +  + G+        R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAAKG------KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQTFVIH 306
             P W  P  RRL+AA+G      K N VVA+DG+GQ+KTI +ALN VPK  N   F+IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK+G+YKE +T+ K + H+  IGDGP KT+I+G+ NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSGTIDFV 426
           +IG ENTAGPE  QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDC VSGT+DF+
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVPVKKQF 486
           FGDA  + Q C  +VRKP   Q C+VTA GR   R+ + L++       D   +P+K   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSYLGRPWKEYSRTIIMESFIDDLIQPEGWLPWAGDWGLKTCFYTEFNCYGPGSDKSKRV 546
           ++YLGRPWKE+SRTIIM++ IDD+I P GWLPW+GD+ LKT +Y E    GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPKHALDFTVGRFILGDRWIKPTGVPYVS 567
           KW GIK +TP+ AL +T  RF+ GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Spg027984.1 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.1 bits (1167), Expect = 1.6e-127
Identity = 264/700 (37.71%), Postives = 373/700 (53.29%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDETTDPATGNKSHEISSSVKLIKDICQPTDYKQ 72
           +Y ++GV++ L++AMV+ V    N     +      ++  ++  +   +  IC  TDYKQ
Sbjct: 3   KYVLLGVTA-LIMAMVICVE--ANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQ 62

Query: 73  ECVESLKSAGTSDPKELVQAGFKAAVKLIQAAANKS-ITLK-EIEKDPRASKALAGCKEL 132
           +C  SL +  + DP+ L+++ F  A+  I++  ++  I LK   + D    +AL  C+EL
Sbjct: 63  DCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCREL 122

Query: 133 MDFAIEELKWSIKRLGEFDITKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEKMK 192
           MD AI++L+ +  +   F  T+L + + D+ +WLS +ITYQ+TC+DGFE     AA  M+
Sbjct: 123 MDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVMME 182

Query: 193 KALKTSMRVSSNGLAMVTQISSMLTDLQIP-----------GI----------------- 252
           + ++    ++SNGLA+   +  +L   +IP           GI                 
Sbjct: 183 RVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQDS 242

Query: 253 ----------------------------------NRR----------------------- 312
                                             N R                       
Sbjct: 243 PPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSAFS 302

Query: 313 -------------------------------------------------------RLLDI 372
                                                                   L D 
Sbjct: 303 GNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLKDR 362

Query: 373 PVLGHDDYPDWANPGMRRLLAAKG-----KPNIVVAKDGSGQYKTIQEALNRVPKRKNNQ 432
            +    ++P W  P  RRLLA +      K N+VVAKDGSG+ KTI +AL  VP  KN +
Sbjct: 363 HLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKNTK 422

Query: 433 TFVIHIKQGVYKEYLTINKTLTHLMLIGDGPKKTIISGNKNFI-DGTPTFKTATVAVTAE 492
            FVIHIK+GVYKE + + K + H+M +GDGP KT+I+G+  F+ D   T++TA+VAV  +
Sbjct: 423 KFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNGD 482

Query: 493 HFMARDIGFENTAGPEKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCVVSG 552
           +FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C VSG
Sbjct: 483 YFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVSG 542

Query: 553 TIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKPDTDLVP 565
           TIDFVFGDA AVFQ C F++R+P+ +QQCIVTA GRK+RR+ + ++I NS    D   +P
Sbjct: 543 TIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYLP 602

The following BLAST results are available for this feature:

BLAST of Spg027984.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880188.1	1.8e-301	87.54	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]
XP_008450602.1	2.0e-300	86.52	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >K...	[more]
XP_004135775.1	2.6e-300	86.69	putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044....	[more]
XP_022969472.1	2.8e-286	84.64	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima]	[more]
XP_022931680.1	2.4e-285	84.29	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata]	[more]

BLAST of Spg027984.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	3.1e-187	55.29	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	6.0e-183	54.03	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	7.4e-149	47.13	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	6.3e-140	44.89	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	2.3e-126	37.71	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Spg027984.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CGC3	9.6e-301	86.52	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180...	[more]
A0A1S3BPI8	9.6e-301	86.52	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1	[more]
A0A0A0LYF5	1.3e-300	86.69	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1	[more]
A0A6J1HWF1	1.3e-286	84.64	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1	[more]
A0A6J1F058	1.1e-285	84.29	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1	[more]

BLAST of Spg027984.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	2.2e-188	55.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	4.3e-184	54.03	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	5.3e-150	47.13	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	4.5e-141	44.89	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	1.6e-127	37.71	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 56..206 e-value: 1.5E-36 score: 137.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 60..206 e-value: 2.5E-25 score: 89.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 56..210 e-value: 1.4E-22 score: 77.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 257..554 e-value: 2.5E-138 score: 459.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 56..211 e-value: 5.1E-33 score: 116.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 59..211
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 251..568 e-value: 2.2E-118 score: 397.3
None	No IPR available	PANTHER	PTHR31707:SF131	PECTINESTERASE	coord: 16..571
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 16..571
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 64..212 e-value: 1.93722E-40 score: 142.192
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 402..411
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 283..302
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 255..567

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg027984	Spg027984	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027984.1-three_prime_utr	Spg027984.1-three_prime_utr-scaffold2:46148720..46149013	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027984.1-cds	Spg027984.1-cds-scaffold2:46149014..46150249	CDS
Spg027984.1-cds	Spg027984.1-cds-scaffold2:46150331..46150843	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027984.1-five_prime_utr	Spg027984.1-five_prime_utr-scaffold2:46150844..46150927	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg027984.1	Spg027984.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity