Spg027237.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg027237.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold8: 3697108 .. 3701577 (+)
Sequence length	1422
RNA-Seq Expression	Spg027237.1
Synteny	Spg027237.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTTTTTAGAAATATTCCTACATTTCAACGGGTTATGCTCACTTTGACAAATCTCTCCCTTTTCGCTCAAAGAAATAAGTGTTTAAGGGTAATTTTGGTATTTGAAATGAAAGATTAGAGCATTAGGGTTGAAACTTCAAAGTTAAAAAATAATCAAAATAAAAACTACTAATTATATATTTAAAATTAAAAGAAAAAAAAAAAAAAAAGGCAATTGAGAGAATAGACATGCGCAAGCGCATTGTCCACCCCTCCACTTTTAGGCCCGTAGCCTTCTCCTCCTCCAACATTCACGTCCATTTCCATAACCCAAATCCCCACTGTTCCATTCTCAACTTTTTTGTCTCTCTCTCTCCTCTCTTCCTTCCATTTTCACATGAACAAATTTCAACGCAGTTTCCCTTTCATTTTGAATGGACGCTGAGAAATCCAAATCCAGTTTCGGCCCTCTCATCAAGCCCTCCTGCTCTTCTCTTCTCCTCTTCATCTTCCTCGCTATTTTCACCCTCCAGATCGCCACCAGAACCCCCTTCCCGCTCTGGCTCGCTTCCGATCCATCCTCGTCGGACTCCGCCTCCGAACGGACCTGCACCGGCTTCTTCCGCCGGGATGCGCCCGCCGCCGCGGTGATGTCGATTACCGATTTCGGAGGAGTGGGCGACGGGAGGACGTCCAACACGGAGGCGTTTCGGAAGGCGATTCAGTACATGCGGCGGTTCGCCGAGAAAGGCGGCTCCAAACTGACCGTTCCCAGGGGCAGGTGGCTCACGGGCACCTTAAATCTCACCAGTAATTTCACGCTTTTCCTAGAACACGGCGCTGTCATTTTGGGCTCACAGGTCTCTTTCTCTCTCTCTTTTTCCTTCTTTTTTTTTTTCATTAGATTAAATTTTCTTTTTGGATTTTTTTTTTTTTTTATAGAAATATTATATCACAATTACCATCTCTAGAAACTACAAATTCCTTAATATTGAACATTGTTCGATTGGGTGAAATGGTGATTTAAGTTTTTTTTTTTTTTTTTTTTTTTACTCATAAGGTTAAATTATCAAAAATACCTCTGAGATTGGTTTCAATTATATCTATGAACTTTTAAAAGTTTGAAATTTGAAGTTTATTTCAAAATAACTCTGGGTATTTTTCATTAAATTTATTTAAAAAAAAAATGATGTTTTAAATGTGTTTTTACTGTTTTTTCACTTATGAGTTCAAATCTATTCATAAACAATAATTCATCATTTTCTGTCTATAAATTTAAAGAAAAAAAACATGCAAGGGTAATTTTGAAACAAACTTCAAGGGTTAAACATTTGAAAGTTGAAGGGTATAATTGAAACCAACCTCGAAGGTATTTTTTATAATTCAACCTAATAAATATTGATTTTTTTTCGAGGAAATAAATAATGATAATTTAACCTAATAAACATTGGTCTGGGTAAACCTTTCTGTGGTGTTTTTTACTGTTGGTTTTTTTTTTTTAAAAAAAAATTATTATTAATAATTTAGGAATGAGTTAGAAAGAGAAAGGACAAGAACTTTGTGATTCTGGGGAAGAAGAAAATGCCAAATGGGTTTTATAAGTTAGCAGAAAAGGAAAAATCAGAGTGATGACTGGCGGTATCACCTATGTTGTTTTTTCATATATTTTAAACCAAAATACATGCCCACTGGGCCCACCATCAACAATGACCTAAATAAATAAATAAATAAATAATAATAATAATAATAATAATAATAAAAAAGCAGTAAAATTATGAGAAAAAAAATTTGCATGCTTTATTGGCATTATGAATTATGATTTAATGCATTCATTAGTGCTTTTGAAGCTAGGCCTTTGATGATGATTTTGTTTTCTTTTTATCTGTTTTTGAAATTGTGTTTATTTCCCTAAAAACTCATAGATGAGAGTAGTATTTATAAACTTAATTTTCGAGAATCAGACGTGGTTATCAAACGGGTCATAGTTTTTACACTTTGTAATCAAATATTATACGGATTCTTTTTGGGAAATGGAAAAAAATAAAAAACAAAATCAAGCTGTAATTCCTTCAACCATAAATGTCAACAGAGCTTTAATGATAACTGGTTGACTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGTATTATTTGAAATTGAATAAACTTTGATTTAATTTATAAAAGAAGTAATGAAATTGAATGAATTGGTTATGAATGATGGTGGCATGTTAGTGTGTGAAAAGTTGAGTTTTAAAAAAGATTTGAATTTGAATTGGTTGAATAAACAAATAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCCAATGTTGTCATTACAGGTACTCCCACTTTTCCTTTTATATATATATATATATATATATATATATATATATATTTGGCTCCATCTTGATTTCTAGGATTTTATATTCATGAAGATTCTTGGTCTTTGTAAAGTAATGGAATGAAACTTTTCTCATGTCAATGGTCTCATTAATACACTTTGATCATCACTAAGCCATTTGGATACAAGATAATAATAAATATTTCATTTTAGGGCCCATGTGACTGATCCCATTTTTATTTCCAAAGATTATGCTTTTTTGCTTTTGCTTTTCATTCTTTTCTATTATAATATCAAATAAGTGTTTCAAATCCTACTTTTTTTTTTTTTTTGTTGGTTAAAATTTGGTGAAGATTTTGTTTTTAATTGGTTAGTATTTGATGAGTACCTCAATTAATTTTCTAAGACCACCAAACCTTAGCTTATGACATTTTTTAGTTGTCGAGAAGATTGTGGGAGTTTATATTATGTGACCACCACGAAGTGAAAAAAGTTAGGTATTTGAATCTCTGAGATGCACGTTTTAAGACCACATCACATGATCCTACGACCTATTACCTTCTTTGAATTTTTTTCACATACCCAAATTGCCACTAGGCCAACCCATAAATGTTAGGTACAAAGAGTTTCAGTATATCTCATATTGAAGTCTTCCCATTTAGCTTTGATTTGCGCTCGGTTCGACGATGTTTTAAATTATACAAATTTGATGAGTTGTGTGTGTTTTCCAGGACAGAATGGAACCATTGATGGTCAAGGCAAGATGTGGTGGGAGCTATGGTGGAACAGGACATTAAATCACACAAGAGGCCACCTTGTAGAGCTCATGAACTCTCACAACATTCTCATCTCCAATCTCACCTTCAAAAACTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGGTTCCGATATCGATCTACTCTTTCTCATTCTATCATTTATCGTATCGTATCGTACCGTAACAAATTTCGAATCATCAAAATTGATCGAGTATTAGATTCACCCATTAAAAACAATGAACTTAGAGATGGACTTGTGTTTGCTGTTTCATTTCAGCAATGTCGTTATAAAAAACATGACGATCTTGGCTCCACTCAACGCCCCGAACACAGACGGTATCGACCCAGATTCGAGTACCAATATCTGTATTGAGGACTGTTACATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAAGGTTGGACGGCCGAGCTCGAACATCGTTATAAGGCGAGTGTCTGGCACAACTCCAAGCTGTTCTGGTGTGGGAATAGGAAGCGAGATGTCGGGTGGAATATCGAACATCACGATCGAGGATTTAAACGTTTGGGATTCGGCAGCTGGGATTCGGATAAAATCGGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAAATAACTTTGTAATGAAGAGAGTGAAGATGGCTATAAGGTTCAGCAGGGGTTCAAATGACCATCCTGATGAAGAATGGGATCCAAAGGCAGTTCCCAAAGTGAAGGGTATTTTCATCAGCAATGTCACGAGCTTCAATTCGACAAAGGCTCCCGTTCTCGATGGCATCGAGGGCACGTCGTTTGACGGTGTATGCATGAAAAACGTCACCATTCTCGGTCTCGCTCCCTCCGCAAAATGGCATTGTGCTTTCGTGTCGGGTTTTAGCACCGCAGTCTTTCCTATGCCATGTCCTCAATTGCAGAACATCACATTCTCATCGTTGTGCTCGTAATTTTTAGCGTCTCTAGGCGCAGTTTTGCTAACTTTTGGGTGTCACACCAGACCGACCGATCGATCGAGAAAGCGCTGCTAGCCAGACATGAGCTACAAAGGTGATCATCACACATGAGATTTAGTTGTCTGGTTGGTTTATGTTCTTCCAATGAAATGTATTATCCCAATACCATCAACATTTTACTGCCTGACCCAACCCAACAATAGGATCTGTTGTACAAAGTAAGGGATCTCAATAGGATCTGTGTTTGTAACATTTGATAGTTATTATTATTATTTTTTTATGGTAATAACAAGAAATTTAGAC

mRNA sequence

ATGGACGCTGAGAAATCCAAATCCAGTTTCGGCCCTCTCATCAAGCCCTCCTGCTCTTCTCTTCTCCTCTTCATCTTCCTCGCTATTTTCACCCTCCAGATCGCCACCAGAACCCCCTTCCCGCTCTGGCTCGCTTCCGATCCATCCTCGTCGGACTCCGCCTCCGAACGGACCTGCACCGGCTTCTTCCGCCGGGATGCGCCCGCCGCCGCGGTGATGTCGATTACCGATTTCGGAGGAGTGGGCGACGGGAGGACGTCCAACACGGAGGCGTTTCGGAAGGCGATTCAGTACATGCGGCGGTTCGCCGAGAAAGGCGGCTCCAAACTGACCGTTCCCAGGGGCAGGTGGCTCACGGGCACCTTAAATCTCACCAGTAATTTCACGCTTTTCCTAGAACACGGCGCTGTCATTTTGGGCTCACAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCCAATGTTGTCATTACAGGACAGAATGGAACCATTGATGGTCAAGGCAAGATGTGGTGGGAGCTATGGTGGAACAGGACATTAAATCACACAAGAGGCCACCTTGTAGAGCTCATGAACTCTCACAACATTCTCATCTCCAATCTCACCTTCAAAAACTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGCAATGTCGTTATAAAAAACATGACGATCTTGGCTCCACTCAACGCCCCGAACACAGACGGTATCGACCCAGATTCGAGTACCAATATCTGTATTGAGGACTGTTACATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAAGGTTGGACGGCCGAGCTCGAACATCGTTATAAGGCGAGTGTCTGGCACAACTCCAAGCTGTTCTGGTGTGGGAATAGGAAGCGAGATGTCGGGTGGAATATCGAACATCACGATCGAGGATTTAAACGTTTGGGATTCGGCAGCTGGGATTCGGATAAAATCGGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAAATAACTTTGTAATGAAGAGAGTGAAGATGGCTATAAGGTTCAGCAGGGGTTCAAATGACCATCCTGATGAAGAATGGGATCCAAAGGCAGTTCCCAAAGTGAAGGGTATTTTCATCAGCAATGTCACGAGCTTCAATTCGACAAAGGCTCCCGTTCTCGATGGCATCGAGGGCACGTCGTTTGACGGTGTATGCATGAAAAACGTCACCATTCTCGGTCTCGCTCCCTCCGCAAAATGGCATTGTGCTTTCGTGTCGGGTTTTAGCACCGCAGTCTTTCCTATGCCATGTCCTCAATTGCAGAACATCACATTCTCATCGTTGTGCTCGTAA

Coding sequence (CDS)

ATGGACGCTGAGAAATCCAAATCCAGTTTCGGCCCTCTCATCAAGCCCTCCTGCTCTTCTCTTCTCCTCTTCATCTTCCTCGCTATTTTCACCCTCCAGATCGCCACCAGAACCCCCTTCCCGCTCTGGCTCGCTTCCGATCCATCCTCGTCGGACTCCGCCTCCGAACGGACCTGCACCGGCTTCTTCCGCCGGGATGCGCCCGCCGCCGCGGTGATGTCGATTACCGATTTCGGAGGAGTGGGCGACGGGAGGACGTCCAACACGGAGGCGTTTCGGAAGGCGATTCAGTACATGCGGCGGTTCGCCGAGAAAGGCGGCTCCAAACTGACCGTTCCCAGGGGCAGGTGGCTCACGGGCACCTTAAATCTCACCAGTAATTTCACGCTTTTCCTAGAACACGGCGCTGTCATTTTGGGCTCACAGGATCCAGATGAGTGGCCCATTATAGAGCCACTTCCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGAAGACACATCAGCCTTATTCATGGCAATGCCCTTTCCAATGTTGTCATTACAGGACAGAATGGAACCATTGATGGTCAAGGCAAGATGTGGTGGGAGCTATGGTGGAACAGGACATTAAATCACACAAGAGGCCACCTTGTAGAGCTCATGAACTCTCACAACATTCTCATCTCCAATCTCACCTTCAAAAACTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGCAATGTCGTTATAAAAAACATGACGATCTTGGCTCCACTCAACGCCCCGAACACAGACGGTATCGACCCAGATTCGAGTACCAATATCTGTATTGAGGACTGTTACATCGAAAGCGGAGATGATCTAGTAGCAGTGAAGAGTGGATGGGATGAATATGGAATCAAGGTTGGACGGCCGAGCTCGAACATCGTTATAAGGCGAGTGTCTGGCACAACTCCAAGCTGTTCTGGTGTGGGAATAGGAAGCGAGATGTCGGGTGGAATATCGAACATCACGATCGAGGATTTAAACGTTTGGGATTCGGCAGCTGGGATTCGGATAAAATCGGACCAAGGTAGAGGAGGATACATTGCAAATGTTAGTATAAATAACTTTGTAATGAAGAGAGTGAAGATGGCTATAAGGTTCAGCAGGGGTTCAAATGACCATCCTGATGAAGAATGGGATCCAAAGGCAGTTCCCAAAGTGAAGGGTATTTTCATCAGCAATGTCACGAGCTTCAATTCGACAAAGGCTCCCGTTCTCGATGGCATCGAGGGCACGTCGTTTGACGGTGTATGCATGAAAAACGTCACCATTCTCGGTCTCGCTCCCTCCGCAAAATGGCATTGTGCTTTCGTGTCGGGTTTTAGCACCGCAGTCTTTCCTATGCCATGTCCTCAATTGCAGAACATCACATTCTCATCGTTGTGCTCGTAA

Protein sequence

MDAEKSKSSFGPLIKPSCSSLLLFIFLAIFTLQIATRTPFPLWLASDPSSSDSASERTCTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWLTGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDGIEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS

Homology

[Close]

BLAST of Spg027237.1 vs. NCBI nr
Match: XP_022946971.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 856.7 bits (2212), Expect = 9.6e-245
Identity = 411/475 (86.53%), Postives = 443/475 (93.26%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRTPFPLWLASDPSSSDSASERTC 60
           MD E  KSSFG LI PSCSS LLLFIF+AI TLQIATRTPFP+W+ S   ++D+ SE TC
Sbjct: 1   MDGE--KSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTC 60

Query: 61  TGFFRRD-APAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           + FF RD AP   VMSITDFGGVGDGRTSNTEAFRKAI+YMRRF +KGGSKL VP+GRW+
Sbjct: 61  SAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLT+NFTLFL+ GAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           V+ITG+NGTIDGQGKMWW+LWWNRTLNHTRGHL+EL+NSHNILISNLT KNSPFWTVHPV
Sbjct: 181 VLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNI+IRRVSGTTP+CSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGYI
Sbjct: 301 RPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           AN+SI+NFVM RVKMAIRFS+GSNDHPDEEWDPKAVP VKGIFISN+TS NSTKAPVL+G
Sbjct: 361 ANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           IEGT FDGVCMKNVTILGLAPSAKWHCAFVSGFS+AVFP PCPQLQNIT SS+CS
Sbjct: 421 IEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

[Close]

BLAST of Spg027237.1 vs. NCBI nr
Match: XP_023533571.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.4 bits (2206), Expect = 4.8e-244
Identity = 410/475 (86.32%), Postives = 442/475 (93.05%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRTPFPLWLASDPSSSDSASERTC 60
           MD E  KSSF  LI PSCSS LLLFIF+A+ TLQIATRTPFP+W+ S   ++D+ SE TC
Sbjct: 1   MDGE--KSSFASLINPSCSSLLLLFIFIAVLTLQIATRTPFPIWMPSSTLTTDTDSEPTC 60

Query: 61  TGFFRRD-APAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           + FF RD AP   VMSITDFGGVGDGRTSNTEAFRKAI+YMRRF EKGGSKL VP+GRW+
Sbjct: 61  SAFFHRDVAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLVVPKGRWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLT+NFTLFL+ GAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           V+ITG+NGTIDGQGKMWW+LWWNRTLNHTRGHL+EL+NSHNILISNLT KNSPFWTVHPV
Sbjct: 181 VLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNI+IRRVSGTTP+CSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGYI
Sbjct: 301 RPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           AN+SI+NFVM RVKMAIRFS+GSNDHPDEEWDPKAVP VKGIFISN+TS NSTKAPVL+G
Sbjct: 361 ANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLNG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           IEGT FDGVCMKNVTILGLAPSAKWHCAFVSGFS+AVFP PCPQLQNIT SS+CS
Sbjct: 421 IEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

[Close]

BLAST of Spg027237.1 vs. NCBI nr
Match: XP_023007164.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-241
Identity = 408/476 (85.71%), Postives = 441/476 (92.65%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRTPFPLWLASDP-SSSDSASERT 60
           MD E  KSSF PLI PSCSS LLLFIF+AI TLQIATRTPFP+W+ S   +++D+ SE T
Sbjct: 1   MDGE--KSSFAPLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLATTDTDSEPT 60

Query: 61  CTGFFRRD-APAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRW 120
           C+ FF RD AP   VM ITDFGGVGDGRTSNTEAFRKAI+YMRRF E GGSKL VP+G W
Sbjct: 61  CSAFFHRDAAPDTVVMLITDFGGVGDGRTSNTEAFRKAIEYMRRFGETGGSKLIVPKGSW 120

Query: 121 LTGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           +TG+ NLT+NFTLFL+ GAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 ITGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHP 240
           NV+ITG+NGTIDGQGKMWW+LWWNRTLNHTRGHL+EL+NSHNILISNLT KNSPFWTVHP
Sbjct: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKV 300
           VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWD+YGI +
Sbjct: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300

Query: 301 GRPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360
            RPSSNI+IRRVSGTTP+CSGVGIGSEMSGGISNITI+DLN+WDSAAG+RIKSDQGRGGY
Sbjct: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNIWDSAAGVRIKSDQGRGGY 360

Query: 361 IANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLD 420
           IAN+SI+NFVM RVKMAIRFS+GSNDHPDEEWDPKAVP VKGIFISN+TS NSTKAPVL+
Sbjct: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLN 420

Query: 421 GIEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           GIEGT FDGVCMKNVTILGLAPSAKWHCAFVSGFS+AVFP PCPQLQNIT SS+CS
Sbjct: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474

[Close]

BLAST of Spg027237.1 vs. NCBI nr
Match: KAA0040757.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 841.3 bits (2172), Expect = 4.2e-240
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRT-PFPLWLASDPSSSDSASERT 60
           MD++KSK  FGP I PSCSS LLLFIFLAI T QIATRT PFP WL   P S       T
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWL---PPSDFQLP--T 60

Query: 61  CTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           C+ FFR D  AA VMSITDFGGVGDG+TSNTEAFRKAIQYMRRF +KGGSKLTVP+G W+
Sbjct: 61  CSAFFRPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLTSNFTLFL+ GA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           VVITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILISNLTFKNSPFWT+HPV
Sbjct: 181 VVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNIVIRRVSGTTP+CSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI
Sbjct: 301 RPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           ANVSI NFVM RVKMAIRFSRGSNDHPDE++DPKAVPKVKGIFI+N+ S NSTKAPVLDG
Sbjct: 361 ANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLDG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           I GTS+DGVCMKNVTILGLAPSAKWHCAFVSGFST+VFPMPCPQLQN TFS LCS
Sbjct: 421 IAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 470

[Close]

BLAST of Spg027237.1 vs. NCBI nr
Match: XP_008460889.1 (PREDICTED: probable polygalacturonase [Cucumis melo])

HSP 1 Score: 841.3 bits (2172), Expect = 4.2e-240
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRT-PFPLWLASDPSSSDSASERT 60
           MD++KSK  FGP I PSCSS LLLFIFLAI T QIATRT PFP WL   P S       T
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWL---PPSDFQLP--T 101

Query: 61  CTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           C+ FFR D  AA VMSITDFGGVGDG+TSNTEAFRKAIQYMRRF +KGGSKLTVP+G W+
Sbjct: 102 CSAFFRPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWV 161

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLTSNFTLFL+ GA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 162 TGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 221

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           VVITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILISNLTFKNSPFWT+HPV
Sbjct: 222 VVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPV 281

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 282 YCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLA 341

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNIVIRRVSGTTP+CSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI
Sbjct: 342 RPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 401

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           ANVSI NFVM RVKMAIRFSRGSNDHPDE++DPKAVPKVKGIFI+N+ S NSTKAPVLDG
Sbjct: 402 ANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLDG 461

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           I GTS+DGVCMKNVTILGLAPSAKWHCAFVSGFST+VFPMPCPQLQN TFS LCS
Sbjct: 462 IAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 511

[Close]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.6e-115
Identity = 206/405 (50.86%), Postives = 277/405 (68.40%), Query Frame = 0


Query: 74  SITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWLTGTLNLTSNFTLFLE 133
           S+ DFGGVGDG+T NT+AF+ A+  + ++  +GG++L VP G+WLTG+ +LTS+FTLFL 
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 134 HGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGQNGTIDGQGK 193
             AV+L SQD  +WP+I+PLPSYGRGR+   GR+ SLI G  L++V+ITG NGTIDGQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 194 MWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIKNMTILAP 253
           +WW+ +    L +TR +L+ELM S +I ISNLT  NSP W VHPVY  N++I+ +TILAP
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAP 243

Query: 254 LNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVIRRVSGTT 313
           + +PNTDGI+PDS TN  IEDCYI SGDD VAVKSGWDEYGI  G P+  +VIRR++  +
Sbjct: 244 VRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCIS 303

Query: 314 PSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSINNFVMKRVKM 373
           P  + + +GSEMSGGI ++  ED+   +S +GIRIK+  GRGGY+ ++ +    MK +K 
Sbjct: 304 PYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKW 363

Query: 374 AIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDGIEGTSFDGVCMKNVT 433
           A   +     H D  +DPKA P ++GI   ++ + N + A  L+GI    F G+C+ NVT
Sbjct: 364 AFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVT 423

Query: 434 ILGLAPSAK---WHCAFVSGFSTAVFPMPCPQL--QNITFSSLCS 474
           I  LA  AK   W C  V G S+ V P PC  L  Q    +SLC+
Sbjct: 424 I-HLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCN 467

[Close]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 9.0e-28
Identity = 115/433 (26.56%), Postives = 188/433 (43.42%), Query Frame = 0


Query: 62  FFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRW---- 121
           FF +   ++  + +T FG VGDG T +++AF KA + +   +  G  +  VP G      
Sbjct: 13  FFLQIFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQ 72

Query: 122 ---LTGTLNLTSNFTLFLEHGAVILGSQ-----DPDEWPIIEPLPSYGRGRERLGGRHIS 181
                G+   T  F   L  G ++  S+     D D+W +                    
Sbjct: 73  PLKFQGSCKSTPVFVQML--GKLVAPSKGNWKGDKDQWILF------------------- 132

Query: 182 LIHGNALSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKN 241
                 +  +VI G +G I+GQG  WWE         +R   ++  + +N+ +S LT  +
Sbjct: 133 ----TDIEGLVIEG-DGEINGQGSSWWE------HKGSRPTALKFRSCNNLRLSGLTHLD 192

Query: 242 SPFWTVHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSG 301
           SP   +H   C+ V I ++ I AP ++PNTDGID  +S+N+ I+DC I +GDD +A+ SG
Sbjct: 193 SPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG 252

Query: 302 WDEYGIKVGRPSSNIVIRRVSGTTPSCSGVGIGSEMSGG----ISNITIEDLNVWDSAAG 361
                      +SNI I  +        G+ IGS    G    + N+ +++ N   +  G
Sbjct: 253 -----------TSNIHISGID--CGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNG 312

Query: 362 IRIKSDQGRGGYIANVSINNFVMKRVKMAI---RFSRGSNDHPDEEWDPKAVPKVKGIFI 421
            RIK+ QG  GY   ++ N   +  V+  I   +F  G +    ++    AV   K +F 
Sbjct: 313 ARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFS 372

Query: 422 SNVTSFNSTKAPVLDGIEGTSFDGVCMKNVTI----LGLAPSAKWHCAFVSGFSTAVFP- 471
           + + +  S         E      + ++++ I     G    A+  C  V G ST   P 
Sbjct: 373 NFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPG 398

[Close]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 7.1e-25
Identity = 101/386 (26.17%), Postives = 172/386 (44.56%), Query Frame = 0


Query: 62  FFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGR-WLTG 121
           F R        +S++DFG  GDG+T +T+AF  A  + +  +  G   L VP+G  +L  
Sbjct: 57  FVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLK 116

Query: 122 TLNLTS--NFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 181
           ++ LT   N  L ++    +  SQ   ++  I               + I     N LS 
Sbjct: 117 SIQLTGPCNSILTVQIFGTLSASQKRSDYKDI--------------SKWIMFDGVNNLS- 176

Query: 182 VVITGQNGTIDGQGKMWWE--LWWNRTLNHTRG-HLVELMNSHNILISNLTFKNSPFWTV 241
            V  G  G +DG G+ WW+     N+    T+    +   NS ++++ NL  +N+    +
Sbjct: 177 -VDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQI 236

Query: 242 HPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGI 301
               CSNV + N+ + AP ++PNTDGI   ++ NI + +  I +GDD ++++SG      
Sbjct: 237 SIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG------ 296

Query: 302 KVGRPSSNIVIRRVSGTTPSCSGVGIGS----EMSGGISNITIEDLNVWDSAAGIRIKSD 361
                S N+ I  +  T     G+ IGS         +S +T++   +  +  G+RIK+ 
Sbjct: 297 -----SQNVQINDI--TCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTY 356

Query: 362 QGRGGYIANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFN-S 421
           QG  G  +N+   N  M  VK  I   +   D      +  AV +VK +   +++  + S
Sbjct: 357 QGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSAS 410

Query: 422 TKAPVLDGIEGTSFDGVCMKNVTILG 437
             A   +  +     G+ +  V I G
Sbjct: 417 ENAITFNCSKNYPCQGIVLDRVNIKG 410

[Close]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.6e-24
Identity = 103/390 (26.41%), Postives = 176/390 (45.13%), Query Frame = 0


Query: 70  AAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRW-------LTGTL 129
           A+ +S+++FG  GDG+T +T+AF+KA  + +  +  G +   VP+G+          G  
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKA--WKKACSTNGVTTFLVPKGKTYLLKSTRFRGPC 124

Query: 130 NLTSNFTLFLEHGAVILGS--QDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVV 189
               NF +     A    S  +D + W I+E +                    N LS  +
Sbjct: 125 KSLRNFQILGTLSASTKRSDYKDKNHWLILEDV--------------------NNLS--I 184

Query: 190 ITGQNGTIDGQGKMWWE--LWWNRTLNHTRG-HLVELMNSHNILISNLTFKNSPFWTVHP 249
             G  G I+G GK WW+     +++   T+    + L N  N+ + NL  KN+    +  
Sbjct: 185 DGGSTGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISI 244

Query: 250 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKV 309
             C+ V + N+ I AP ++PNTDGI   ++ NI + +  I +GDD ++++ G        
Sbjct: 245 EKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDG-------- 304

Query: 310 GRPSSNIVIRRVSGTTPSCSGVGIGS----EMSGGISNITIEDLNVWDSAAGIRIKSDQG 369
              + N+ I  +  T     G+ IGS         +S I ++     +S  G+RIK+ QG
Sbjct: 305 ---TQNLQIFDL--TCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQG 364

Query: 370 RGGYIANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKA 429
             G   N+   N  M+ VK  I   +   D  D+  D ++  +VK +   N++  ++T  
Sbjct: 365 GSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDV 416

Query: 430 PV-LDGIEGTSFDGVCMKNVTILGLAPSAK 443
            + L+  E     G+ ++NV I G   S K
Sbjct: 425 AITLNCSEKYPCQGIVLENVKIKGGTASCK 416

[Close]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.7e-22
Identity = 97/378 (25.66%), Postives = 170/378 (44.97%), Query Frame = 0


Query: 73  MSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGR-WLTGTLNLTS--NFT 132
           +S+ DFG  GDG T +T+AF KA     + A   GS L VP  + +L   +  +      
Sbjct: 85  ISVDDFGARGDG-TDDTKAFEKA----WKDACSSGSVLIVPENKNYLLKQITFSGPCKSD 144

Query: 133 LFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGQNGTID 192
           L ++    I  S D  +W         G  R+R        I    +SN+ + G  GT +
Sbjct: 145 LRVKIRGTIEASSDQSDW--------VGHNRKR-------WIEFEDISNLTLEG-GGTSN 204

Query: 193 GQGKMWWELWWNRTLN---HTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIK 252
           G G+ WW+    R  +    +    +   +  N+++S+L+ K+S    +    C +V+  
Sbjct: 205 GNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIAS 264

Query: 253 NMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVI 312
           N+ + AP ++PNTDGI    +  I + +  I +GDD ++++SG            S +VI
Sbjct: 265 NLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESG------------SKMVI 324

Query: 313 RRVSGTTPSCS---GVGIGS----EMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIA 372
                T  +C    G+ IGS         +S + ++  N++D+  G+RIK+ QG  G   
Sbjct: 325 ----ATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAK 384

Query: 373 NVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPV-LDG 432
           N+   N VM  V   I   +   D  D   + ++  KV  +   N+   ++++  V  D 
Sbjct: 385 NIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDC 425

Query: 433 IEGTSFDGVCMKNVTILG 437
            + +   G  + N+ ++G
Sbjct: 445 SKSSPCQGYIVGNINLVG 425

[Close]

BLAST of Spg027237.1 vs. ExPASy TrEMBL
Match: A0A6J1G526 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.7e-245
Identity = 411/475 (86.53%), Postives = 443/475 (93.26%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRTPFPLWLASDPSSSDSASERTC 60
           MD E  KSSFG LI PSCSS LLLFIF+AI TLQIATRTPFP+W+ S   ++D+ SE TC
Sbjct: 1   MDGE--KSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTC 60

Query: 61  TGFFRRD-APAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           + FF RD AP   VMSITDFGGVGDGRTSNTEAFRKAI+YMRRF +KGGSKL VP+GRW+
Sbjct: 61  SAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLT+NFTLFL+ GAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           V+ITG+NGTIDGQGKMWW+LWWNRTLNHTRGHL+EL+NSHNILISNLT KNSPFWTVHPV
Sbjct: 181 VLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNI+IRRVSGTTP+CSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGYI
Sbjct: 301 RPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           AN+SI+NFVM RVKMAIRFS+GSNDHPDEEWDPKAVP VKGIFISN+TS NSTKAPVL+G
Sbjct: 361 ANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           IEGT FDGVCMKNVTILGLAPSAKWHCAFVSGFS+AVFP PCPQLQNIT SS+CS
Sbjct: 421 IEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

[Close]

BLAST of Spg027237.1 vs. ExPASy TrEMBL
Match: A0A6J1L474 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.2e-242
Identity = 408/476 (85.71%), Postives = 441/476 (92.65%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRTPFPLWLASDP-SSSDSASERT 60
           MD E  KSSF PLI PSCSS LLLFIF+AI TLQIATRTPFP+W+ S   +++D+ SE T
Sbjct: 1   MDGE--KSSFAPLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLATTDTDSEPT 60

Query: 61  CTGFFRRD-APAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRW 120
           C+ FF RD AP   VM ITDFGGVGDGRTSNTEAFRKAI+YMRRF E GGSKL VP+G W
Sbjct: 61  CSAFFHRDAAPDTVVMLITDFGGVGDGRTSNTEAFRKAIEYMRRFGETGGSKLIVPKGSW 120

Query: 121 LTGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           +TG+ NLT+NFTLFL+ GAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 ITGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHP 240
           NV+ITG+NGTIDGQGKMWW+LWWNRTLNHTRGHL+EL+NSHNILISNLT KNSPFWTVHP
Sbjct: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKV 300
           VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWD+YGI +
Sbjct: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300

Query: 301 GRPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360
            RPSSNI+IRRVSGTTP+CSGVGIGSEMSGGISNITI+DLN+WDSAAG+RIKSDQGRGGY
Sbjct: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNIWDSAAGVRIKSDQGRGGY 360

Query: 361 IANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLD 420
           IAN+SI+NFVM RVKMAIRFS+GSNDHPDEEWDPKAVP VKGIFISN+TS NSTKAPVL+
Sbjct: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLN 420

Query: 421 GIEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           GIEGT FDGVCMKNVTILGLAPSAKWHCAFVSGFS+AVFP PCPQLQNIT SS+CS
Sbjct: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474

[Close]

BLAST of Spg027237.1 vs. ExPASy TrEMBL
Match: A0A5A7TGP3 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold703G00210 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRT-PFPLWLASDPSSSDSASERT 60
           MD++KSK  FGP I PSCSS LLLFIFLAI T QIATRT PFP WL   P S       T
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWL---PPSDFQLP--T 60

Query: 61  CTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           C+ FFR D  AA VMSITDFGGVGDG+TSNTEAFRKAIQYMRRF +KGGSKLTVP+G W+
Sbjct: 61  CSAFFRPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLTSNFTLFL+ GA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           VVITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILISNLTFKNSPFWT+HPV
Sbjct: 181 VVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNIVIRRVSGTTP+CSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI
Sbjct: 301 RPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           ANVSI NFVM RVKMAIRFSRGSNDHPDE++DPKAVPKVKGIFI+N+ S NSTKAPVLDG
Sbjct: 361 ANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLDG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           I GTS+DGVCMKNVTILGLAPSAKWHCAFVSGFST+VFPMPCPQLQN TFS LCS
Sbjct: 421 IAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 470

[Close]

BLAST of Spg027237.1 vs. ExPASy TrEMBL
Match: A0A1S3CDX7 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 412/475 (86.74%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRT-PFPLWLASDPSSSDSASERT 60
           MD++KSK  FGP I PSCSS LLLFIFLAI T QIATRT PFP WL   P S       T
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWL---PPSDFQLP--T 101

Query: 61  CTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           C+ FFR D  AA VMSITDFGGVGDG+TSNTEAFRKAIQYMRRF +KGGSKLTVP+G W+
Sbjct: 102 CSAFFRPDVTAAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWV 161

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLTSNFTLFL+ GA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 162 TGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 221

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           VVITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILISNLTFKNSPFWT+HPV
Sbjct: 222 VVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPV 281

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 282 YCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLA 341

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNIVIRRVSGTTP+CSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI
Sbjct: 342 RPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 401

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           ANVSI NFVM RVKMAIRFSRGSNDHPDE++DPKAVPKVKGIFI+N+ S NSTKAPVLDG
Sbjct: 402 ANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLDG 461

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           I GTS+DGVCMKNVTILGLAPSAKWHCAFVSGFST+VFPMPCPQLQN TFS LCS
Sbjct: 462 IAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 511

[Close]

BLAST of Spg027237.1 vs. ExPASy TrEMBL
Match: A0A0A0LJW9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 8.5e-239
Identity = 410/475 (86.32%), Postives = 434/475 (91.37%), Query Frame = 0


Query: 1   MDAEKSKSSFGPLIKPSCSS-LLLFIFLAIFTLQIATRT-PFPLWLASDPSSSDSASERT 60
           MD++KSK  FGP I PSCSS LLLFIFLAI T QIATRT PFP WL   P S       T
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWL---PPSDFQLP--T 60

Query: 61  CTGFFRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWL 120
           C+ FFR DA A  VMSITDFGGVGDG+TSNTEAFRKAIQYMRRF +KGGSKLTVP+G W+
Sbjct: 61  CSAFFRPDATAGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSWV 120

Query: 121 TGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180
           TG+ NLTSNFTLFL+ GA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN
Sbjct: 121 TGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 180

Query: 181 VVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPV 240
           VVITG+NGTIDGQGKMWWELWWNRTLNHTRGHLVEL+NSHNILISNLTFKNSPFWT+HPV
Sbjct: 181 VVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHPV 240

Query: 241 YCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVG 300
           YCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YGI + 
Sbjct: 241 YCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINLA 300

Query: 301 RPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360
           RPSSNIVIRRVSGTTP+CSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI
Sbjct: 301 RPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYI 360

Query: 361 ANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDG 420
           ANVSI NFVM RVKMAIRFSRGSNDHPDE++DPKAVPKVKGIFI+N+ S NSTKAPVL G
Sbjct: 361 ANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLYG 420

Query: 421 IEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNITFSSLCS 474
           I GTS+DGVCMKNVTILGL PSAKWHCAFVSGFST+VFP PCPQLQN TFSSLCS
Sbjct: 421 IAGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPTPCPQLQNTTFSSLCS 470

[Close]

BLAST of Spg027237.1 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 681.8 bits (1758), Expect = 4.0e-196
Identity = 325/466 (69.74%), Postives = 393/466 (84.33%), Query Frame = 0


Query: 1   MDAEKSKSS-FGPLIKPSCSSLLL-FIFLAIFTLQIATRTPFPLWLASDPSSSDSASERT 60
           M+ EK+ ++    + +PS + LLL F  LAI +LQI++ +  PLW+     +S      T
Sbjct: 1   MEPEKNPTTVLSAITRPSWAFLLLVFTVLAILSLQISSNSFLPLWI----PTSQYDDPVT 60

Query: 61  CTGFFRRDA-PAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRW 120
           C+GFF  D  P   VMSITDFGGVGDG+TSNT AFR+A++++  FA +GG++L VP G W
Sbjct: 61  CSGFFNHDPFPNRIVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTW 120

Query: 121 LTGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           L+G+ NLTSNFTLFLE GA+ILGS+D DEWPIIEPLPSYGRGRER GGRHISLIHG+ L+
Sbjct: 121 LSGSFNLTSNFTLFLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLT 180

Query: 181 NVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHP 240
           NVVITG+NGTIDGQGKMWWELWWNRTL HTRGHL+EL NSHNILISNLT  NSPFWT+HP
Sbjct: 181 NVVITGENGTIDGQGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKV 300
           VYCSNVVI+NMTILAP+NAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWD+YG+ V
Sbjct: 241 VYCSNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAV 300

Query: 301 GRPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360
            RPSSNIVIRR+SGTT +CSGVGIGSEMSGGI NIT+ED++VWDSAAG+RIK+D+GRGGY
Sbjct: 301 ARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGY 360

Query: 361 IANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLD 420
           I+N++ NN ++++VK+ IRFS GSNDH D++WDPKA+P+VKGI+ISNV S NS KAP+L 
Sbjct: 361 ISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLL 420

Query: 421 GIEGTSFDGVCMKNVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQL 464
           G+EGTSF  VC++NVT+LGL  + KW C  VSG+++ VFP+ CPQL
Sbjct: 421 GVEGTSFQDVCLRNVTLLGLPKTEKWKCKDVSGYASDVFPLSCPQL 462

[Close]

BLAST of Spg027237.1 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 4.8e-133
Identity = 228/399 (57.14%), Postives = 294/399 (73.68%), Query Frame = 0


Query: 73  MSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWLTGTLNLTSNFTLFL 132
           +SIT+FG VGDG T NT+AF+ A+ Y+  F++KGG+KL VP G+WLTG+ +L S+ TL+L
Sbjct: 43  VSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWL 102

Query: 133 EHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGQNGTIDGQG 192
           + GA ILGS   + WP+++PLPSYGRGRE  G RH SLI+G  L++VVITG+NGTIDGQG
Sbjct: 103 DKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQG 162

Query: 193 KMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIKNMTILA 252
            +WW+ + N  LN+TR HLVELMNS  ++ISNLTF NSPFW +HPVYC +VV+KN+TILA
Sbjct: 163 TVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILA 222

Query: 253 PLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVIRRVSGT 312
           PL +PNTDG+DPDSSTN+CIEDCYI +GDDLV++KSGWDEYGI   RPSS I I R++G 
Sbjct: 223 PLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQ 282

Query: 313 TPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSINNFVMKRVK 372
           T S SG+ IGSEMSGG+S I I+DL++++S  GIRIK+  GRGGY+ NV I N  +  VK
Sbjct: 283 TTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVK 342

Query: 373 MAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDGIEGTSFDGVCMKNV 432
            AIRF+    +HPDE++DPKA+P ++ I   NV       A +L+GIEG  F  +C  NV
Sbjct: 343 KAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNV 402

Query: 433 TILGLAPSAK--WHCAFVSGFSTAVFP-MPCPQLQNITF 469
           T+     S K  W C+ V G+S  V P + C  L+   F
Sbjct: 403 TLRVKKNSKKSPWECSNVRGYSQWVSPEITCDSLKESIF 441

[Close]

BLAST of Spg027237.1 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 3.4e-131
Identity = 230/430 (53.49%), Postives = 303/430 (70.47%), Query Frame = 0


Query: 49  SSSDSASERTCTGF----FRRDAPAAAVMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAE 108
           SSS   SE TC+G     +R D      +SITDFGGVGDGRT NT+AFR AI  ++    
Sbjct: 18  SSSLGDSEATCSGIVPLRYRYDK-----ISITDFGGVGDGRTVNTKAFRAAIYRIQHLKR 77

Query: 109 KGGSKLTVPRGRWLTGTLNLTSNFTLFLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLG 168
           +GG+ L +P G +LT + NLTS+ TL+L  GAVI   QD   WP+I+PLPSYGRGRE  G
Sbjct: 78  RGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGRELPG 137

Query: 169 GRHISLIHGNALSNVVITGQNGTIDGQGKMWWELWWNRTLNHTRGHLVELMNSHNILISN 228
           GR++S IHG+ L +VVITGQNGTIDGQG++WW +W +RTL +TR +L+E  +S  I+ISN
Sbjct: 138 GRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDSKEIIISN 197

Query: 229 LTFKNSPFWTVHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLV 288
           + F+NSPFW +HPVYCSNVVI ++TILAP ++PNTDGIDPDSS N+CIED YI +GDDLV
Sbjct: 198 VIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDDLV 257

Query: 289 AVKSGWDEYGIKVGRPSSNIVIRRVSGTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAA 348
           A+KSGWD+YGI  GRPSSNI IRR++G++P  +G+ IGSE SGGI NI  E + + +   
Sbjct: 258 AIKSGWDQYGIAYGRPSSNITIRRITGSSP-FAGIAIGSETSGGIKNIIAEHITLSNMGV 317

Query: 349 GIRIKSDQGRGGYIANVSINNFVMKRVKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISN 408
           G+ IK++ GRGGYI N+ I++  +   K  I+ +  + DHPDE ++P A+P VKGI I N
Sbjct: 318 GVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIHIKN 377

Query: 409 VTSFNSTKAPVLDGIEGTSFDGVCMKNVTILGLAPSAK-WHCAFVSGFSTAVFPMPCPQL 468
           V   N   A  + G++G+ F G+C+  + + G   S K W C+ VSG S  V P PC +L
Sbjct: 378 VWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVSPWPCSEL 437

Query: 469 QNITFSSLCS 474
           +    S+LCS
Sbjct: 438 RTTGGSNLCS 441

[Close]

BLAST of Spg027237.1 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.2e-121
Identity = 206/397 (51.89%), Postives = 285/397 (71.79%), Query Frame = 0


Query: 72  VMSITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKG-GSKLTVPRGRWLTGTLNLTSNFTL 131
           ++SI+DFG VGDG+T NT+AF  AI  +R       G+ L VPRG +LT + NLTS+ TL
Sbjct: 44  MLSISDFGAVGDGKTLNTKAFNSAIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTL 103

Query: 132 FLEHGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVVITGQNGTIDG 191
           +L  GAVI   QD ++WP+ +PLPSYGRGRE  G R+IS IHG+ L++VVITG+NGTIDG
Sbjct: 104 YLADGAVIKAVQDTEKWPLTDPLPSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDG 163

Query: 192 QGKMWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIKNMTI 251
           QG+ WW +W + TL  TR  L+E  NS NIL+S++  +NSPFWT+HPVYCSNVV+ ++TI
Sbjct: 164 QGEPWWNMWRHGTLKFTRPGLIEFNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTI 223

Query: 252 LAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVIRRVS 311
           LAP ++ NTDGIDPDSS+N+CIED YI +GDDLVAVKSGWDEYGI   RPS +I IRR++
Sbjct: 224 LAPTDSYNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRIT 283

Query: 312 GTTPSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSINNFVMKR 371
           G++P  +G+ IGSE SGGI N+T+E++ +++S  GI IK++ GRGG I  ++I+   +++
Sbjct: 284 GSSP-FAGIAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEK 343

Query: 372 VKMAIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDGIEGTSFDGVCMK 431
           V+  I+ S  + DHPD++++  A+P V+GI I NV      +A ++ G++ + F  +C  
Sbjct: 344 VRTGIKISGDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFS 403

Query: 432 NVTILGLAPSAKWHCAFVSGFSTAVFPMPCPQLQNIT 468
           NVT+ G   S  W C+ V G +  V P PCP+L   T
Sbjct: 404 NVTLTGTKRSPIWKCSDVVGAADKVNPTPCPELSATT 439

[Close]

BLAST of Spg027237.1 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 9.6e-118
Identity = 202/393 (51.40%), Postives = 276/393 (70.23%), Query Frame = 0


Query: 75  ITDFGGVGDGRTSNTEAFRKAIQYMRRFAEKGGSKLTVPRGRWLTGTLNLTSNFTLFLEH 134
           +TDFG VGDG+TSNT+AFR AI  + + A  GG++L VP G+WLTG+ NLTS+FTLF++ 
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 135 GAVILGSQDPDEWPIIEPLPSYGRGRERLG-GRHISLIHGNALSNVVITGQNGTIDGQGK 194
           GA IL SQD  EWP+I PLPSYG+GR+  G GR  SLI G  L++VVITG NGTI+GQG+
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 195 MWWELWWNRTLNHTRGHLVELMNSHNILISNLTFKNSPFWTVHPVYCSNVVIKNMTILAP 254
            WW+ +  +    TR +L+E++ S NI ISN+T  +SP W +HPVYC++V++K++T+LAP
Sbjct: 167 YWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAP 226

Query: 255 LNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDEYGIKVGRPSSNIVIRRVSGTT 314
           +  PNTDGI+PDS TN  IEDCYI SGDD +AVKSGWD+YGIK G P+  + IRR++  +
Sbjct: 227 VTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCIS 286

Query: 315 PSCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGYIANVSINNFVMKRVKM 374
           P  +GV +GSEMSGGI ++ IED+ + ++ + IRIK+  GRG Y+ ++      MK +K 
Sbjct: 287 PKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKY 346

Query: 375 AIRFSRGSNDHPDEEWDPKAVPKVKGIFISNVTSFNSTKAPVLDGIEGTSFDGVCMKNVT 434
               S     HPDE +DPKA+P++  I   ++T+ N T +  LDGI+   F G+C+ NVT
Sbjct: 347 VFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVT 406

Query: 435 ILGLAPSAK---WHCAFVSGFSTAVFPMPCPQL 464
           I  LA  AK   W+C  V+G ++ V P PC  L
Sbjct: 407 I-ALAAKAKKMQWNCTDVAGVTSRVTPEPCSLL 438

The following BLAST results are available for this feature:

BLAST of Spg027237.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946971.1	9.6e-245	86.53	probable polygalacturonase [Cucurbita moschata]	[more]
XP_023533571.1	4.8e-244	86.32	probable polygalacturonase [Cucurbita pepo subsp. pepo]	[more]
XP_023007164.1	1.7e-241	85.71	probable polygalacturonase [Cucurbita maxima]	[more]
KAA0040757.1	4.2e-240	86.74	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
XP_008460889.1	4.2e-240	86.74	PREDICTED: probable polygalacturonase [Cucumis melo]	[more]

BLAST of Spg027237.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	2.6e-115	50.86	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q9LW07	9.0e-28	26.56	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q8RY29	7.1e-25	26.17	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
O23147	1.6e-24	26.41	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]
Q02096	5.7e-22	25.66	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]

BLAST of Spg027237.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G526	4.7e-245	86.53	probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV...	[more]
A0A6J1L474	8.2e-242	85.71	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1	[more]
A0A5A7TGP3	2.0e-240	86.74	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3CDX7	2.0e-240	86.74	probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1	[more]
A0A0A0LJW9	8.5e-239	86.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1	[more]

BLAST of Spg027237.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33440.1	4.0e-196	69.74	Pectin lyase-like superfamily protein	[more]
AT3G62110.1	4.8e-133	57.14	Pectin lyase-like superfamily protein	[more]
AT4G23820.1	3.4e-131	53.49	Pectin lyase-like superfamily protein	[more]
AT5G41870.1	3.2e-121	51.89	Pectin lyase-like superfamily protein	[more]
AT3G48950.1	9.6e-118	51.40	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 329..350 e-value: 27.0 score: 13.7 coord: 267..299 e-value: 160.0 score: 11.1 coord: 240..266 e-value: 680.0 score: 8.4 coord: 301..323 e-value: 4600.0 score: 2.5 coord: 397..431 e-value: 1900.0 score: 5.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 54..449 e-value: 7.2E-116 score: 389.5
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 169..448 e-value: 6.3E-44 score: 150.4
None	No IPR available	PANTHER	PTHR31339:SF4	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 13..472
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 13..472
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 67..464

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg027237	Spg027237	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027237.1-five_prime_utr	Spg027237.1-five_prime_utr-scaffold8:3697108..3697523	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027237.1-cds	Spg027237.1-cds-scaffold8:3697524..3697949	CDS
Spg027237.1-cds	Spg027237.1-cds-scaffold8:3699386..3699506	CDS
Spg027237.1-cds	Spg027237.1-cds-scaffold8:3700240..3700414	CDS
Spg027237.1-cds	Spg027237.1-cds-scaffold8:3700569..3701268	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg027237.1-three_prime_utr	Spg027237.1-three_prime_utr-scaffold8:3701269..3701577	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg027237.1	Spg027237.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004650	polygalacturonase activity