Spg026128.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg026128.1 vs. NCBI nr
Match: XP_022149008.1 (cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 840.9 bits (2171), Expect = 6.0e-240
Identity = 416/511 (81.41%), Postives = 454/511 (88.85%), Query Frame = 0

Query: 9   TTVLLLGLLCA-SIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMK 68
           T V LLG+L A SIFY    + LKL WKP RI+R MRSQG+ GPPS FIYGN+ Q+MKMK
Sbjct: 5   TAVFLLGVLSASSIFY----VLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMK 64

Query: 69  MESMRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRE 128
            ESMRTSLD +DH+ILPRV+PHIHLWI+KYGKNF+QWNG  AQLIVSDPETIKEILHNRE
Sbjct: 65  FESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRE 124

Query: 129 RAIPKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVE 188
           RAI K+ LG D RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVE
Sbjct: 125 RAITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVE 184

Query: 189 KWKKQPEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGI 248
           KWKK  EGKE+DLFEEFK+LTSDIIAHTAFGSSYLEGKFIFETL+KL+MI FKNQFKR I
Sbjct: 185 KWKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRI 244

Query: 249 PGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDP 308
           PG SKLF++KDAREGDELE +MK+SI+SIIK REEKV+SGEADNYGNDFL LLLKAKNDP
Sbjct: 245 PGFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDP 304

Query: 309 DQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENP 368
           D RQRI V+DVVDECKT+YFAGQETT VLLSW +FLLATHV WQEEARKEV DMFGN NP
Sbjct: 305 DPRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNP 364

Query: 369 TLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDS 428
           TLEGI KLKIMSMIIKE+LRLYPPA  MSRMVKKEVRLGKLVLPA+IQVSI  IAVHHD+
Sbjct: 365 TLEGIAKLKIMSMIIKESLRLYPPATPMSRMVKKEVRLGKLVLPASIQVSISNIAVHHDT 424

Query: 429 AIWGEDAHEFKPERFSEGKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFS 488
           A WGEDAHEFKPERFSEG A+DIPS AYIPFGLGPRNCVGN+LA+NETKI LSMILQRFS
Sbjct: 425 ATWGEDAHEFKPERFSEGTAKDIPSAAYIPFGLGPRNCVGNILAVNETKIALSMILQRFS 484

Query: 489 FTISPGHVHAPYQLLTNCPQHGVRVKLQSLT 519
           FTISP +VHAP+Q LT CPQHGV+VK+  L+
Sbjct: 485 FTISPAYVHAPFQFLTICPQHGVQVKIHPLS 511

BLAST of Spg026128.1 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 615.5 bits (1586), Expect = 4.1e-172
Identity = 303/504 (60.12%), Postives = 377/504 (74.80%), Query Frame = 0

Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLD 76
           L   + +   KLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LFGCLLFGVIKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYTKRMQAMATPMD 72

Query: 77  FTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLG 136
            + H+ILPRV P +H W+N YG+NFLQW G  AQLI++DPE IKE+LH+R++  PK  L 
Sbjct: 73  LS-HHILPRVMPSVHSWLNLYGRNFLQWFGVDAQLIITDPEMIKEVLHDRQKNFPKAKLQ 132

Query: 137 EDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
             + RIFGNG  T+EGE+WAK R+IA++AFHG+ LK M PTM+ C E M E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGERWAKSRKIANFAFHGDSLKNMIPTMIECGEKMTEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRS 256
           E+D+F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ +S
Sbjct: 193 ELDVFKEFKVYTLDVISHTAFGSSYQQGKDIFHMLQELCELSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVE 316
           KD  EG+ LE++MK   + IIK REEK+ +GEAD YGNDFL LL+KAKNDP+  QRI VE
Sbjct: 253 KDDYEGERLEKRMKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKNDPEISQRISVE 312

Query: 317 DVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLK 376
           D+VDECKT YFAG ETT VLL+W  FLLA H  WQEEAR EVL +FG++NPTLEG+PKL+
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMFLLALHKEWQEEARNEVLKVFGDKNPTLEGLPKLR 372

Query: 377 IMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHE 436
           +M +II E LRLYPPA  +SR+V+KEVRLG LV+P   ++ IPT+AVHHD   WGEDAHE
Sbjct: 373 MMMIIINECLRLYPPAMTVSRLVEKEVRLGNLVVPTMTKLVIPTVAVHHDMTFWGEDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 496
           FKPERF E  GKA +  S AYIPFGLGPRNCVG   A+NE KI +SMILQR+SF +SP +
Sbjct: 433 FKPERFLEGVGKATESNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFDLSPAY 492

Query: 497 VHAPYQLLTNCPQHGVRVKLQSLT 519
           VH P QLLT  PQ+GV+V L S+T
Sbjct: 493 VHMPAQLLTISPQNGVQVILNSIT 514

BLAST of Spg026128.1 vs. NCBI nr
Match: XP_008446466.1 (PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 2.5e-169
Identity = 292/503 (58.05%), Postives = 374/503 (74.35%), Query Frame = 0

Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLD 76
           L   + ++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  FTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLG 136
            + HNILPRV P +H W+N YG+N+LQW G  AQL+++DPE IKE+LH+R+++ PK  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
             + RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ +S
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVE 316
           KD  EG+ LE+++K   + IIK REEK+ + EA +YGNDFL LL+KAKNDP+  Q I +E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLK 376
           D+VDECKT YFAG ETT VLL+W   LLA H  WQE+AR EV D+FG+ +PTLEG+PKLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHE 436
            M+MII E LRLYPPA  ++R V+KEVRLG LV+P    ++IPT+AVHHD+  WG+DAHE
Sbjct: 373 AMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 496
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 433 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 492

Query: 497 VHAPYQLLTNCPQHGVRVKLQSL 518
            H P QLLT CPQ+GV+V L S+
Sbjct: 493 AHMPAQLLTICPQNGVQVILNSI 513

BLAST of Spg026128.1 vs. NCBI nr
Match: XP_022149052.1 (cytochrome P450 CYP749A22-like [Momordica charantia] >XP_022149053.1 cytochrome P450 CYP749A22-like [Momordica charantia])

HSP 1 Score: 604.7 bits (1558), Expect = 7.2e-169
Identity = 291/495 (58.79%), Postives = 376/495 (75.96%), Query Frame = 0

Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRV 86
           KLF+KL W P RI+RFMRSQGIQGP   FI GN  ++   +  ++ T +D + H ILPRV
Sbjct: 23  KLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMHTRRTHAIATPIDLS-HRILPRV 82

Query: 87  HPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGEDVRRIFGNG 146
            PH H W+++YG+NF+QW G  AQLI++DPE I+E+LH+R++  PK  L   + +IFGNG
Sbjct: 83  LPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHDRQKKFPKAKLHGFLNKIFGNG 142

Query: 147 HLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKV 206
            +T+EGE WAK R+IA++AFHG+ LK M P M+ CAE M+E+WK   EGKE+D+F+  KV
Sbjct: 143 LVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETMMEEWKHY-EGKELDVFQHLKV 202

Query: 207 LTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELE 266
            TSD+I+HTAFGSSY +GK IF+ L  L  +  +N FK  +PGI K+F+SKD  EG+ LE
Sbjct: 203 YTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIRLPGIRKIFKSKDDVEGERLE 262

Query: 267 RKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMY 326
           ++MK   + +IK+REEK I+GEAD YGNDFL LL+KAKNDP++ QRI V+D+VDECKT Y
Sbjct: 263 KRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVDECKTFY 322

Query: 327 FAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLKIMSMIIKETL 386
           FAG ETT VLL+W  FLLA H  WQ++AR EV  +FG++NPT EG+PKLK M+MI+ E L
Sbjct: 323 FAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEGLPKLKTMTMILYECL 382

Query: 387 RLYPPAQVMSRMV-KKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERFSEG 446
           RLYPPA  + R V +KEVRLG LVLPA I+V+IPT AVHHD+A WGEDA EFKPERF+EG
Sbjct: 383 RLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHHDTAYWGEDASEFKPERFAEG 442

Query: 447 KAEDIP--SGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQLLT 506
            A+ I   S AY+PFGLG R+CVG   A+ E KI +SMILQR+SFT+SP + H+P Q LT
Sbjct: 443 VAKAIERNSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMILQRYSFTLSPAYAHSPVQFLT 502

Query: 507 NCPQHGVRVKLQSLT 519
            CPQHG+++ L S++
Sbjct: 503 ICPQHGLQLILHSIS 515

BLAST of Spg026128.1 vs. NCBI nr
Match: XP_004135430.2 (cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetical protein Csa_008223 [Cucumis sativus])

HSP 1 Score: 604.0 bits (1556), Expect = 1.2e-168
Identity = 295/503 (58.65%), Postives = 375/503 (74.55%), Query Frame = 0

Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLD 76
           L   + +  FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMD 72

Query: 77  FTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLG 136
            + HNIL RV P +H W+N YG+N+LQW+G  AQL+++DPE IKE+LH+R+++ PK  L 
Sbjct: 73  LS-HNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
             + RIFGNG  T+EG++WA  RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ +S
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKS 252

Query: 257 KDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVE 316
           KD  EG+ L +++K   + IIK REEK+ +GEA++YGNDFL LL+KAKNDP+  Q I +E
Sbjct: 253 KDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQCISME 312

Query: 317 DVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLK 376
           D+VDECKT YFAG ETT VLL+W   LLA H  WQE+AR EVLD+FG+ NPTLEG+PKLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHE 436
            M+MII E LRLYPPA  ++R V+KEVRLG LV+P    ++IPT+AVHHD+  WGEDAHE
Sbjct: 373 TMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 496
           FKPERFSE  GKA +  S AYIPFGLGPRNCVG   A+NE KI +SMILQR+SF +SP +
Sbjct: 433 FKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPAY 492

Query: 497 VHAPYQLLTNCPQHGVRVKLQSL 518
            H P QLLT  PQ+GV+V L S+
Sbjct: 493 AHMPAQLLTISPQNGVQVILNSI 513

BLAST of Spg026128.1 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.7e-150
Identity = 255/508 (50.20%), Postives = 362/508 (71.26%), Query Frame = 0

Query: 13  LLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMR 72
           LL  LC  + ++F +    + W P R++R  R QGI+GP   F+YGN  +I+ M+ ESM 
Sbjct: 19  LLSSLCVFLLFVFIRFLNDIWWTPIRLQRVFRQQGIRGPSYGFLYGNTKEILNMRKESMS 78

Query: 73  TSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPK 132
             +D+  HNI PR+ PH++ W+N YGKNFL W G  AQ +V+  + +KE +  +++A PK
Sbjct: 79  RPMDYLSHNIFPRLQPHLYSWLNIYGKNFLNWYGPRAQFVVTQVDFVKETM-IKDQAYPK 138

Query: 133 VTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQ 192
           +      +++ G+G +TS+G+KWAK RR+A++AFH E LK M P M++  E M+++W KQ
Sbjct: 139 MDPEWFAKKLLGDGIVTSKGKKWAKHRRLANHAFHAESLKSMTPAMIASVEMMLKRW-KQ 198

Query: 193 PEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISK 252
            EG+E+D+F+EFK+LTS++I+ TAFGSSYL+GK IF+ LT+L +I  +N +K  +PGIS 
Sbjct: 199 HEGREIDVFQEFKILTSEVISRTAFGSSYLDGKDIFDRLTQLGIIITRNSYKVKLPGISL 258

Query: 253 LFRSKDAREGDELERKMKSSIISIIKNRE-EKVISGEADNYGNDFLALLLKAKNDPDQRQ 312
            ++S D  E ++L++ +  SI+ I++ RE E  +SGE  ++G DFL LL+KA ND D++ 
Sbjct: 259 FYKSNDEIEAEKLDQGLYDSILRIMEKREKESTMSGEVGSFGTDFLGLLMKAMNDADEKN 318

Query: 313 RIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEG 372
           RI  +DVVDECKT Y AGQETT  LL+W  FLL  H  WQE+AR+EVL++FG E P  +G
Sbjct: 319 RITAQDVVDECKTFYVAGQETTTTLLAWVIFLLGIHTDWQEKARQEVLNLFGQEIPNSDG 378

Query: 373 IPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWG 432
           + KLK ++MII ETLRLYPP   ++R VK+E + GKL LPAN+ + +PT+A+HHD  IWG
Sbjct: 379 LAKLKTVNMIINETLRLYPPVIFLTRKVKEETKFGKLTLPANVHIVVPTLALHHDEQIWG 438

Query: 433 EDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFT 492
           +DA  FKPERFS+G  KA +  + A+ PFGLGPR+CVG   A NE KI L+MILQ +SF 
Sbjct: 439 DDALLFKPERFSQGVAKATNNNAAAFFPFGLGPRSCVGLNFATNEAKIALAMILQCYSFA 498

Query: 493 ISPGHVHAPYQLLTNCPQHGVRVKLQSL 518
           +SP ++H+P Q+LT  PQHG++V LQ L
Sbjct: 499 LSPTYIHSPVQILTVRPQHGLQVMLQPL 524

BLAST of Spg026128.1 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.2e-97
Identity = 206/518 (39.77%), Postives = 305/518 (58.88%), Query Frame = 0

Query: 12  LLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQ--IMKMKME 71
           ++L ++ A+I      +   L  +P R+ R +R+QG+ G P      N  Q  ++K++ E
Sbjct: 12  VVLTVILAAIPIWVCHMVNTLWLRPKRLERHLRAQGLHGDPYKLSLDNSKQTYMLKLQQE 71

Query: 72  SMRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERA 131
           +   S+  +  +  PR+    H  ++KYGKN   W G   ++I++DPE IKE+  N+ + 
Sbjct: 72  AQSKSIGLSKDDAAPRIFSLAHQTVHKYGKNSFAWEGTAPKVIITDPEQIKEVF-NKIQD 131

Query: 132 IPKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKW 191
            PK  L   + +    G +  EG+KWAK R+I + AFH E LK M P        M+ KW
Sbjct: 132 FPKPKL-NPIAKYISIGLVQYEGDKWAKHRKIINPAFHLEKLKGMLPAFSHSCHEMISKW 191

Query: 192 K--KQPEGK-EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRG 251
           K     +G  EVD++   + LT D+I+ TAFGSSY EG  IFE L +        ++ R 
Sbjct: 192 KGLLSSDGTCEVDVWPFLQNLTCDVISRTAFGSSYAEGAKIFELLKRQGYALMTARYAR- 251

Query: 252 IPGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKA--- 311
           IP +  L  S   R   E+ER ++ S+  II+ RE+ + SG++ +  +D L +LL++   
Sbjct: 252 IP-LWWLLPSTTKRRMKEIERGIRDSLEGIIRKREKALKSGKSTD--DDLLGILLQSNHI 311

Query: 312 KNDPDQRQR---IFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLD 371
           +N  D+  +   +  ++V++ECK  Y AGQETT  LL+W   LL  H  WQ  AR+EVL 
Sbjct: 312 ENKGDENSKSAGMTTQEVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQ 371

Query: 372 MFGNENPTLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPT 431
           +FGN+NP  EG+ +LKI++MI+ E LRLYPP   ++R ++K+++LG L+LPA +QVS+P 
Sbjct: 372 VFGNQNPNFEGLGRLKIVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPI 431

Query: 432 IAVHHDSAIWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITL 491
           + +HHD  IWG DA EF PERF+EG A+       Y PFG GPR CVG   A+ E KI L
Sbjct: 432 LLIHHDEGIWGNDAKEFNPERFAEGIAKATKGQVCYFPFGWGPRICVGQNFALLEAKIVL 491

Query: 492 SMILQRFSFTISPGHVHAPYQLLTNCPQHGVRVKLQSL 518
           S++LQ FSF +SP + H P  +LT  P+HG  + L  L
Sbjct: 492 SLLLQNFSFELSPTYAHVPTTVLTLQPKHGAPIILHKL 523

BLAST of Spg026128.1 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 8.5e-96
Identity = 199/518 (38.42%), Postives = 309/518 (59.65%), Query Frame = 0

Query: 11  VLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMES 70
           VL+L ++ + +      L   L W+P +I      QGI+GPP  F  GN+ +++ M +++
Sbjct: 13  VLVLSVILSLVIVKGMSL---LWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 72

Query: 71  MRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAI 130
               + F+ HNILPRV    H W   YG  FL W G   +L V+DP+ I+EI  ++    
Sbjct: 73  SSHPMPFS-HNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFY 132

Query: 131 PKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKW- 190
            K      V+++ G+G L+ +GEKWA  R+I    FH E LK + P ++     MV+KW 
Sbjct: 133 EKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWS 192

Query: 191 -KKQPEGK-EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGI 250
            K    G+ EVD++E F++LT D+I+ TAFGSSY +G+ +F    +  ++  +   K  I
Sbjct: 193 DKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 252

Query: 251 PGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADN----YGNDFLALLLKA 310
           PG  + F ++   +  +L+++++ S++ +I+ R +  I GE +        D L L+++A
Sbjct: 253 PGY-RFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA 312

Query: 311 KNDPDQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFG 370
           KN       + V+D+V+ECK+ +FAG++TT  LL+W   LL+ H  WQ +AR EVL + G
Sbjct: 313 KN-------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCG 372

Query: 371 NEN-PTLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIA 430
           + + PT + + KLK +SMI+ E+LRLYPP     R  K +V+LG   +P   ++ IP IA
Sbjct: 373 SRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIA 432

Query: 431 VHHDSAIWGEDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLS 490
           VHHD AIWG D +EF P RF++G  +A   P G +IPFGLG R C+G  LAI + K+TL+
Sbjct: 433 VHHDQAIWGNDVNEFNPARFADGVPRAAKHPVG-FIPFGLGVRTCIGQNLAILQAKLTLA 492

Query: 491 MILQRFSFTISPGHVHAPYQLLTNCPQHGVRVKLQSLT 519
           +++QRF+F ++P + HAP  L+   PQHG  +  + LT
Sbjct: 493 VMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Spg026128.1 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.9e-95
Identity = 199/496 (40.12%), Postives = 290/496 (58.47%), Query Frame = 0

Query: 36  PFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRVHPHIHLWIN 95
           P ++   +R QG +G      YG+L +  +M  ++    ++ +D  +L RV P IH  + 
Sbjct: 36  PRKLEESLRKQGFRGNSYRLFYGDLKESSEMTRKAKLKPINLSDDPVL-RVRPFIHQTVK 95

Query: 96  KYGKNFLQWNGNMAQLIVSDPETIKEIL-----HNRERAIPKVTLGEDVRRIFGNGHLTS 155
           KYGK+   W G   ++ + DPE IKEI+      N+ +  P V       ++F +G    
Sbjct: 96  KYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSYKFNKPKRNPLV-------KLFADGLANH 155

Query: 156 EGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKK---QPEGKEVDLFEEFKVL 215
           EGE WAK R++ + AFH E LK M P M      MV KW K   +   +E+D++   + L
Sbjct: 156 EGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKWDKMISKDGSRELDVWPFLQRL 215

Query: 216 TSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELER 275
           TSD+I+HTAFGSSY EG  +FE  T+ A +  K      IPG S L  +K  R+  E++R
Sbjct: 216 TSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQSVYIPGWSYL-PTKRNRKMKEIDR 275

Query: 276 KMKSSIISIIKNREEKVISGEADNYGNDFLALLLKA--KNDPDQRQR---IFVEDVVDEC 335
           K +S +++II  + + + +GE     +D L +LL++  K    Q ++   + +++V+ EC
Sbjct: 276 KTQSCLMNIINKKTKAMQAGEGST--DDILGILLESNLKEQLGQGKKNVGMSIQEVMGEC 335

Query: 336 KTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLKIMSMII 395
           K  YFAGQETT  LL W   LL+ H +WQ  AR+EVL  FGN  P  + +  LKI++MI+
Sbjct: 336 KQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQFGNAKPDFDNLNHLKIVTMIL 395

Query: 396 KETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERF 455
            E LRLYPP   + R V +E  LG + LPA +Q+S+P + +HHD  IWG+DA EF PERF
Sbjct: 396 YEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMILHHDQNIWGDDAKEFNPERF 455

Query: 456 SEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQL 515
           SEG ++   +   + PFG GPR C+G   A+ E K+ L++ILQRFSF +SP + HAP  +
Sbjct: 456 SEGVSKATKNQVVFFPFGWGPRICIGQNFALLEAKLALAIILQRFSFELSPSYTHAPTTV 515

Query: 516 LTNCPQHGVRVKLQSL 518
           LT  PQHG  + L  L
Sbjct: 516 LTVQPQHGANLILHKL 520

BLAST of Spg026128.1 vs. ExPASy Swiss-Prot
Match: Q05047 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.0e-93
Identity = 198/510 (38.82%), Postives = 289/510 (56.67%), Query Frame = 0

Query: 26  FKLFLKLCWK--------PFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDF 85
           F L +   W+        P RI + +R QG +G P  F+ G++ +  KM  E++   ++F
Sbjct: 17  FALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALSKPMEF 76

Query: 86  TDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGE 145
            +++I+PR+ PHI+  IN YG+N   W G + ++ V +PE IKE+L +  +      +  
Sbjct: 77  -NNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNFDVHN 136

Query: 146 DVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPE--- 205
            + +    G  + EG KW+K RRI   AF  E LK M P    C   M+ KW+K  E   
Sbjct: 137 PLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIAEKQG 196

Query: 206 GKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLF 265
             EVD+F  F VLTSD+I+  AFGS+Y EG  IF  L +L  +         IPG S L 
Sbjct: 197 SHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGWSYLP 256

Query: 266 RSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQ--- 325
             ++ R   E+ +++   +  II  R + + +GE     +D L +LL++     Q+Q   
Sbjct: 257 TKRNKRM-KEINKEITDMLRFIINKRMKALKAGEPGE--DDLLGVLLESNIQEIQKQGNK 316

Query: 326 ---RIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPT 385
               + + DV++ECK  YFAGQETTGVLL+W   LL+ H  WQE AR+EVL  FG   P 
Sbjct: 317 KDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKPE 376

Query: 386 LEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSA 445
            E +  LK +SMI+ E LRLYPP   ++++V K+ +LG   +PA  QV +PT+ +H + +
Sbjct: 377 FERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREKS 436

Query: 446 IWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLSMILQRFS 505
           IWGEDA EF P RF +G A    +   Y+PF  GPR C+G   A+ + K+ L+MILQRF 
Sbjct: 437 IWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRFK 496

Query: 506 FTISPGHVHAPYQLLTNCPQHGVRVKLQSL 518
           F ++P +VHAP+ +LT  PQ G  V  + L
Sbjct: 497 FDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of Spg026128.1 vs. ExPASy TrEMBL
Match: A0A6J1D5N2 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017532 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.9e-240
Identity = 416/511 (81.41%), Postives = 454/511 (88.85%), Query Frame = 0

Query: 9   TTVLLLGLLCA-SIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMK 68
           T V LLG+L A SIFY    + LKL WKP RI+R MRSQG+ GPPS FIYGN+ Q+MKMK
Sbjct: 5   TAVFLLGVLSASSIFY----VLLKLWWKPLRIQRIMRSQGVYGPPSNFIYGNMIQVMKMK 64

Query: 69  MESMRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRE 128
            ESMRTSLD +DH+ILPRV+PHIHLWI+KYGKNF+QWNG  AQLIVSDPETIKEILHNRE
Sbjct: 65  FESMRTSLDISDHHILPRVNPHIHLWIDKYGKNFIQWNGTQAQLIVSDPETIKEILHNRE 124

Query: 129 RAIPKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVE 188
           RAI K+ LG D RRIFG+G  TS+GEKWAK RRIADYAFHGELLK M PTMVSCAEAMVE
Sbjct: 125 RAITKIDLGGDARRIFGDGLSTSDGEKWAKARRIADYAFHGELLKNMMPTMVSCAEAMVE 184

Query: 189 KWKKQPEGKEVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGI 248
           KWKK  EGKE+DLFEEFK+LTSDIIAHTAFGSSYLEGKFIFETL+KL+MI FKNQFKR I
Sbjct: 185 KWKKNDEGKELDLFEEFKLLTSDIIAHTAFGSSYLEGKFIFETLSKLSMILFKNQFKRRI 244

Query: 249 PGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDP 308
           PG SKLF++KDAREGDELE +MK+SI+SIIK REEKV+SGEADNYGNDFL LLLKAKNDP
Sbjct: 245 PGFSKLFKTKDAREGDELEMRMKNSIVSIIKKREEKVMSGEADNYGNDFLGLLLKAKNDP 304

Query: 309 DQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENP 368
           D RQRI V+DVVDECKT+YFAGQETT VLLSW +FLLATHV WQEEARKEV DMFGN NP
Sbjct: 305 DPRQRISVDDVVDECKTVYFAGQETTNVLLSWTSFLLATHVDWQEEARKEVFDMFGNTNP 364

Query: 369 TLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDS 428
           TLEGI KLKIMSMIIKE+LRLYPPA  MSRMVKKEVRLGKLVLPA+IQVSI  IAVHHD+
Sbjct: 365 TLEGIAKLKIMSMIIKESLRLYPPATPMSRMVKKEVRLGKLVLPASIQVSISNIAVHHDT 424

Query: 429 AIWGEDAHEFKPERFSEGKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFS 488
           A WGEDAHEFKPERFSEG A+DIPS AYIPFGLGPRNCVGN+LA+NETKI LSMILQRFS
Sbjct: 425 ATWGEDAHEFKPERFSEGTAKDIPSAAYIPFGLGPRNCVGNILAVNETKIALSMILQRFS 484

Query: 489 FTISPGHVHAPYQLLTNCPQHGVRVKLQSLT 519
           FTISP +VHAP+Q LT CPQHGV+VK+  L+
Sbjct: 485 FTISPAYVHAPFQFLTICPQHGVQVKIHPLS 511

BLAST of Spg026128.1 vs. ExPASy TrEMBL
Match: A0A1S3BF46 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 1.2e-169
Identity = 292/503 (58.05%), Postives = 374/503 (74.35%), Query Frame = 0

Query: 17  LCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLD 76
           L   + ++ FKLF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D
Sbjct: 13  LSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMD 72

Query: 77  FTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLG 136
            + HNILPRV P +H W+N YG+N+LQW G  AQL+++DPE IKE+LH+R+++ PK  L 
Sbjct: 73  LS-HNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 132

Query: 137 EDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGK 196
             + RIFGNG  T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGK
Sbjct: 133 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGK 192

Query: 197 EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRS 256
           E+D F+EFKV T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ +S
Sbjct: 193 ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKS 252

Query: 257 KDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVE 316
           KD  EG+ LE+++K   + IIK REEK+ + EA +YGNDFL LL+KAKNDP+  Q I +E
Sbjct: 253 KDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISME 312

Query: 317 DVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLK 376
           D+VDECKT YFAG ETT VLL+W   LLA H  WQE+AR EV D+FG+ +PTLEG+PKLK
Sbjct: 313 DIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLK 372

Query: 377 IMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHE 436
            M+MII E LRLYPPA  ++R V+KEVRLG LV+P    ++IPT+AVHHD+  WG+DAHE
Sbjct: 373 AMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 432

Query: 437 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 496
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 433 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 492

Query: 497 VHAPYQLLTNCPQHGVRVKLQSL 518
            H P QLLT CPQ+GV+V L S+
Sbjct: 493 AHMPAQLLTICPQNGVQVILNSI 513

BLAST of Spg026128.1 vs. ExPASy TrEMBL
Match: A0A6J1D6R8 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 3.5e-169
Identity = 291/495 (58.79%), Postives = 376/495 (75.96%), Query Frame = 0

Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRV 86
           KLF+KL W P RI+RFMRSQGIQGP   FI GN  ++   +  ++ T +D + H ILPRV
Sbjct: 23  KLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMHTRRTHAIATPIDLS-HRILPRV 82

Query: 87  HPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGEDVRRIFGNG 146
            PH H W+++YG+NF+QW G  AQLI++DPE I+E+LH+R++  PK  L   + +IFGNG
Sbjct: 83  LPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHDRQKKFPKAKLHGFLNKIFGNG 142

Query: 147 HLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKV 206
            +T+EGE WAK R+IA++AFHG+ LK M P M+ CAE M+E+WK   EGKE+D+F+  KV
Sbjct: 143 LVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETMMEEWKHY-EGKELDVFQHLKV 202

Query: 207 LTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELE 266
            TSD+I+HTAFGSSY +GK IF+ L  L  +  +N FK  +PGI K+F+SKD  EG+ LE
Sbjct: 203 YTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIRLPGIRKIFKSKDDVEGERLE 262

Query: 267 RKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMY 326
           ++MK   + +IK+REEK I+GEAD YGNDFL LL+KAKNDP++ QRI V+D+VDECKT Y
Sbjct: 263 KRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKNDPEESQRISVDDIVDECKTFY 322

Query: 327 FAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLKIMSMIIKETL 386
           FAG ETT VLL+W  FLLA H  WQ++AR EV  +FG++NPT EG+PKLK M+MI+ E L
Sbjct: 323 FAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKNPTSEGLPKLKTMTMILYECL 382

Query: 387 RLYPPAQVMSRMV-KKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERFSEG 446
           RLYPPA  + R V +KEVRLG LVLPA I+V+IPT AVHHD+A WGEDA EFKPERF+EG
Sbjct: 383 RLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHHDTAYWGEDASEFKPERFAEG 442

Query: 447 KAEDIP--SGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQLLT 506
            A+ I   S AY+PFGLG R+CVG   A+ E KI +SMILQR+SFT+SP + H+P Q LT
Sbjct: 443 VAKAIERNSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMILQRYSFTLSPAYAHSPVQFLT 502

Query: 507 NCPQHGVRVKLQSLT 519
            CPQHG+++ L S++
Sbjct: 503 ICPQHGLQLILHSIS 515

BLAST of Spg026128.1 vs. ExPASy TrEMBL
Match: A0A5D3CAL6 (Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00230 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 6.2e-166
Identity = 289/503 (57.46%), Postives = 369/503 (73.36%), Query Frame = 0

Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRV 86
           +LF+KL W P RI+RFMRSQGIQGP   FI GN   +   +M++M T +D + HNILPRV
Sbjct: 427 QLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPMDLS-HNILPRV 486

Query: 87  HPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGEDVRRIFGNG 146
            P +H W+N YG+N+LQW G  AQL+++DPE IKE+LH+R+++ PK  L   + RIFGNG
Sbjct: 487 MPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIDRIFGNG 546

Query: 147 HLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKV 206
             T+EG++W   RRIA++AFHG+ LK M PTM+ C E M+E WK   EGKE+D F+EFKV
Sbjct: 547 LATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNY-EGKELDAFKEFKV 606

Query: 207 LTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELE 266
            T D+I+HTAFGSSY +GK IF  L +L  +  +N +K  +P ISK+ +SKD  EG+ LE
Sbjct: 607 FTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKSKDDYEGERLE 666

Query: 267 RKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMY 326
           +++K   + IIK REEK+ + EA +YGNDFL LL+KAKNDP+  Q I +ED+VDECKT Y
Sbjct: 667 KRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISMEDIVDECKTFY 726

Query: 327 FAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNENPTLEGIPKLKI--------- 386
           FAG ETT VLL+W   LLA H  WQE+AR EV D+FG+ +PTLEG+PKLK          
Sbjct: 727 FAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLKADVCVRFYYG 786

Query: 387 -MSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHE 446
            M+MII E LRLYPPA  ++R V+KEVRLG LV+P    ++IPT+AVHHD+  WG+DAHE
Sbjct: 787 QMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDTKFWGDDAHE 846

Query: 447 FKPERFSE--GKAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGH 506
           FKPERFSE  GKA +  S AY+PFGLGPRNCVG   A NE KI +SMILQR+SF++SP +
Sbjct: 847 FKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQRYSFSLSPAY 906

Query: 507 VHAPYQLLTNCPQHGVRVKLQSL 518
            H P QLLT CPQ+GV+V L S+
Sbjct: 907 AHMPAQLLTICPQNGVQVILNSI 927

BLAST of Spg026128.1 vs. ExPASy TrEMBL
Match: K7NBW0 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-3 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 5.2e-165
Identity = 285/495 (57.58%), Postives = 374/495 (75.56%), Query Frame = 0

Query: 27  KLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRV 86
           KL ++L   P RI++ MRSQGI+GPP  FI GN  ++   +M++  T +D + HNILPRV
Sbjct: 23  KLVVRLWMSPIRIQKTMRSQGIRGPPYQFIQGNTKEMYTKRMQATATPMDLS-HNILPRV 82

Query: 87  HPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGEDVRRIFGNG 146
            PH+H W+N YGKNFLQW G  AQ +V+DPE IKE+LH R+R  PK  L   +++IFG+G
Sbjct: 83  LPHVHSWLNHYGKNFLQWLGTEAQFVVTDPEMIKEMLHVRQRNFPKAKLHGYIQKIFGDG 142

Query: 147 HLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKV 206
            +T+EGE+WAK R+IA+++F+G+ LK M P M  CAEAM+E+WK   EGKE+D+FE+ K+
Sbjct: 143 TVTAEGERWAKLRKIANFSFNGDSLKSMIPAMTGCAEAMIERWKHY-EGKEIDVFEQMKI 202

Query: 207 LTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELE 266
            TSD+I+HTAFGSSY +GK IF TL +++ ++ +N +K   P IS++F+ KD  EG+ L+
Sbjct: 203 YTSDVISHTAFGSSYEQGKHIFYTLQQISELYIRNAYKVSFPVISRIFKHKDHIEGERLQ 262

Query: 267 RKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMY 326
           ++MK   + +IK RE K +SGEAD YGNDFL LL+KAKNDP+  QRI V+++VDECKT Y
Sbjct: 263 KRMKECFMKVIKTREAKFLSGEADGYGNDFLGLLVKAKNDPEISQRISVDEIVDECKTFY 322

Query: 327 FAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFG-NENPTLEGIPKLKIMSMIIKET 386
           FAG ETT +LL+W  FLLA H  WQEEARKE   +FG ++NPT EG+ KLK M+MII E 
Sbjct: 323 FAGHETTSMLLTWTMFLLALHQEWQEEARKEAFRIFGDSDNPTAEGLAKLKTMTMIINEC 382

Query: 387 LRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERFSEG 446
           LRLYPPA  +SR V+KEVRLG+LVLP  I V++PTIAVH D+  WGEDAH FKPERFSEG
Sbjct: 383 LRLYPPAMTVSRTVEKEVRLGRLVLPKTITVAVPTIAVHQDTEYWGEDAHVFKPERFSEG 442

Query: 447 KAEDIPSGA--YIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQLLT 506
            A+ I S +  Y+PFGLGPR CVG   A+NE KI +SMIL+R+SFT+SP + H+P QL+T
Sbjct: 443 IAKVIESNSAGYLPFGLGPRICVGMNFALNEAKIVISMILRRYSFTLSPAYSHSPIQLVT 502

Query: 507 NCPQHGVRVKLQSLT 519
            CPQHG++V L  L+
Sbjct: 503 VCPQHGLQVILHPLS 515

BLAST of Spg026128.1 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 6.0e-97
Identity = 199/518 (38.42%), Postives = 309/518 (59.65%), Query Frame = 0

Query: 11  VLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMES 70
           VL+L ++ + +      L   L W+P +I      QGI+GPP  F  GN+ +++ M +++
Sbjct: 13  VLVLSVILSLVIVKGMSL---LWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKA 72

Query: 71  MRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAI 130
               + F+ HNILPRV    H W   YG  FL W G   +L V+DP+ I+EI  ++    
Sbjct: 73  SSHPMPFS-HNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFY 132

Query: 131 PKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKW- 190
            K      V+++ G+G L+ +GEKWA  R+I    FH E LK + P ++     MV+KW 
Sbjct: 133 EKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWS 192

Query: 191 -KKQPEGK-EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGI 250
            K    G+ EVD++E F++LT D+I+ TAFGSSY +G+ +F    +  ++  +   K  I
Sbjct: 193 DKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 252

Query: 251 PGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADN----YGNDFLALLLKA 310
           PG  + F ++   +  +L+++++ S++ +I+ R +  I GE +        D L L+++A
Sbjct: 253 PGY-RFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA 312

Query: 311 KNDPDQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFG 370
           KN       + V+D+V+ECK+ +FAG++TT  LL+W   LL+ H  WQ +AR EVL + G
Sbjct: 313 KN-------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCG 372

Query: 371 NEN-PTLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIA 430
           + + PT + + KLK +SMI+ E+LRLYPP     R  K +V+LG   +P   ++ IP IA
Sbjct: 373 SRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIA 432

Query: 431 VHHDSAIWGEDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLS 490
           VHHD AIWG D +EF P RF++G  +A   P G +IPFGLG R C+G  LAI + K+TL+
Sbjct: 433 VHHDQAIWGNDVNEFNPARFADGVPRAAKHPVG-FIPFGLGVRTCIGQNLAILQAKLTLA 492

Query: 491 MILQRFSFTISPGHVHAPYQLLTNCPQHGVRVKLQSLT 519
           +++QRF+F ++P + HAP  L+   PQHG  +  + LT
Sbjct: 493 VMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Spg026128.1 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 345.9 bits (886), Expect = 5.6e-95
Identity = 197/517 (38.10%), Postives = 306/517 (59.19%), Query Frame = 0

Query: 7   SDTTVLLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKM 66
           S + V  L +L A +    +++   +  KP  +   ++ QG+ G P T + G++ + + M
Sbjct: 2   SFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDM 61

Query: 67  KMESMRTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNR 126
            ME+    ++ TD +I PR+ P     +N +GK F  W G +  +++++PE IKE+  N+
Sbjct: 62  MMEARSKPINVTD-DITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVF-NK 121

Query: 127 ERAIPKVTLGEDVRRIFGNGHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMV 186
                K +    +R + G G  + +G+KWA  RRI + AFH E +K M P    C   +V
Sbjct: 122 VNDFEKASTFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVV 181

Query: 187 EKWKKQPEGK----EVDLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQ 246
            +W+K    K    EVD++     +T+D+I+HTAFGSSY EG+ IF+   +LA +  +  
Sbjct: 182 CQWEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAF 241

Query: 247 FKRGIPGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLK 306
            K  IPG S+ + +K  R    ++R++   +  I+  RE+   +GE  N  +D L +LL+
Sbjct: 242 KKSYIPG-SRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPAN--DDLLGILLE 301

Query: 307 AKNDPDQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMF 366
           + ++  Q   + VEDV+ ECK  YFAGQETT VLL W   LL+ H  WQ  AR+EV+ + 
Sbjct: 302 SNSEESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVL 361

Query: 367 G-NENPTLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTI 426
           G N  P +E +  LK+M+MI  E LRLYPP   + R+V KE++LG+L LPA IQ+ +PTI
Sbjct: 362 GENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTI 421

Query: 427 AVHHDSAIWGEDAHEFKPERFSEGKAEDIPSG-AYIPFGLGPRNCVGNMLAINETKITLS 486
            V  D+ +WG+DA +FKPERF +G ++   +  ++ PFG GPR C+G   A+ E K+ ++
Sbjct: 422 LVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMA 481

Query: 487 MILQRFSFTISPGHVHAPYQLLTNCPQHGVRVKLQSL 518
           +ILQ+FSF +SP +VHAP  ++T  PQ G  + L  L
Sbjct: 482 LILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Spg026128.1 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 342.8 bits (878), Expect = 4.8e-94
Identity = 185/484 (38.22%), Postives = 281/484 (58.06%), Query Frame = 0

Query: 36  PFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRVHPHIHLWIN 95
           P RI++FM  QGI GP    + GN+  I KM   S         HNI+PR+ PH   W  
Sbjct: 33  PRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSK 92

Query: 96  KYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGED-VRRIFGNGHLTSEGEK 155
           +YGK F+ WNG   +L +++ E IKE+L        K  L +   +   G G L + GE 
Sbjct: 93  QYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEA 152

Query: 156 WAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLFEEFKVLTSDIIAH 215
           W   R +A  AF  + LK     MV C + M E+ +K+  G+EV++ EE + LT+DII+ 
Sbjct: 153 WHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRKE-VGEEVEIGEEMRRLTADIISR 212

Query: 216 TAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELERKMKSSII 275
           T FGSS  +GK +F  LT L  +  +       PG S+   SK  RE   L+ +++  ++
Sbjct: 213 TEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG-SRFLPSKYNREIKSLKTEVERLLM 272

Query: 276 SIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMYFAGQETTG 335
            II +R++ V  G + +YG+D L LLL   +    +  + V+ ++DECKT +F G ETT 
Sbjct: 273 EIIDSRKDSVEIGRSSSYGDDLLGLLLNQMD--SNKNNLNVQMIMDECKTFFFTGHETTS 332

Query: 336 VLLSWAAFLLATHVHWQEEARKEVLDMFGNEN-PTLEGIPKLKIMSMIIKETLRLYPPAQ 395
           +LL+W   LLA +  WQ+  R EV  + G +  P++E +  L  ++ +I E+LRLYPPA 
Sbjct: 333 LLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPAT 392

Query: 396 VMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERFSEGKAEDIPSG 455
           ++ RM  ++++LG L++P  + + IP +A+HH + +WGEDA+EF PERF+        S 
Sbjct: 393 LLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFAS--SR 452

Query: 456 AYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQLLTNCPQHGVRVK 515
            ++PF  GPRNC+G   A+ E KI L+M++ +FSF IS  + HAP  +LT  P++GV++ 
Sbjct: 453 HFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLV 510

Query: 516 LQSL 518
           L+ L
Sbjct: 513 LKPL 510

BLAST of Spg026128.1 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 342.0 bits (876), Expect = 8.1e-94
Identity = 198/518 (38.22%), Postives = 295/518 (56.95%), Query Frame = 0

Query: 12  LLLGLLCASIFYLFFKLFLKLCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESM 71
           L L +L   I    FK F+ L W PF + R +++QGI GP     YGNL +I KMK ES 
Sbjct: 7   LALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESH 66

Query: 72  RTSLDFTDHNILPRVHPHIHLWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIP 131
            + LD + ++I PR+ PH   W+++YG+ FL WNG   ++ +SDPE  K +L N+     
Sbjct: 67  LSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKLGFFV 126

Query: 132 KVTLGEDVRRIFGN-GHLTSEGEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWK 191
           K     +  ++ G+ G +  EG  W + RRI + AF  + LK M   MV C   M+E+W+
Sbjct: 127 KSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEEWR 186

Query: 192 KQPEGKEV-------DLFEEFKVLTSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQF 251
           K+   +E        ++ EEF+ LT+DIIA +AFGSSY+EG  +F +  +L   +  +  
Sbjct: 187 KESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLN 246

Query: 252 KRGIPGISKLFRSKDAREGDELERKMKSSIISIIKNREEKVISGEADNYGNDFLALLLKA 311
           +  IPG   L    + R   +LERKM +SI  II +R +        +YG+D L +LLKA
Sbjct: 247 QVSIPGTQYLPTPSNIRVW-KLERKMDNSIKRIISSRLQ-----SKSDYGDDLLGILLKA 306

Query: 312 KNDPDQRQRIFVEDVVDECKTMYFAGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFG 371
            N   + +++ +E+++ EC+T +F G ETT  LL+W   LL+ H  WQE+ R+E+    G
Sbjct: 307 YNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECG 366

Query: 372 NE-NPTLEGIPKLKIMSMIIKETLRLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIA 431
            E  P  E   KLK+M+M+I E+LRLY P   ++R     ++LG L +P    V IP + 
Sbjct: 367 KEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLK 426

Query: 432 VHHDSAIWGEDAHEFKPERFSEG--KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLS 491
           +H D  +WG DA +F P RF+ G  +A + P+ A + F +GPR C+G    + E K  L+
Sbjct: 427 MHSDKTLWGSDADKFNPMRFANGVSRAANHPN-ALLAFSVGPRACIGQNFVMIEAKTVLT 486

Query: 492 MILQRFSF-TISPGHVHAPYQLLTNCPQHGVRVKLQSL 518
           MILQRF F ++   + H P   +T  PQ+G+ V LQ L
Sbjct: 487 MILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Spg026128.1 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-93
Identity = 189/495 (38.18%), Postives = 282/495 (56.97%), Query Frame = 0

Query: 32  LCWKPFRIRRFMRSQGIQGPPSTFIYGNLFQIMKMKMESMRTSLDFTDHNILPRVHPHIH 91
           L W+P+ + R  + QGI GP    +YGNL +I KMK E+    LD   ++I+PRV PH+ 
Sbjct: 84  LVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQ 143

Query: 92  LWINKYGKNFLQWNGNMAQLIVSDPETIKEILHNRERAIPKVTLGEDVRRIFGNGHLTSE 151
            W ++YG+ FL W G   +L +SD E  K+IL N+     K     ++ ++ GNG +   
Sbjct: 144 QWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILKLSGNGLIFVN 203

Query: 152 GEKWAKGRRIADYAFHGELLKKMGPTMVSCAEAMVEKWKKQPEGKEVDLF----EEFKVL 211
           G  W + RRI + AF  + LK M   MV C   M  +WKKQ  G E + F     EFK L
Sbjct: 204 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 263

Query: 212 TSDIIAHTAFGSSYLEGKFIFETLTKLAMIFFKNQFKRGIPGISKLFRSKDAREGDELER 271
           T+DIIA  AFGSSY EG  +F++  +L             PGI  L    + +   +L+ 
Sbjct: 264 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIW-KLDM 323

Query: 272 KMKSSIISIIKNREEKVISGEADNYGNDFLALLLKAKNDPDQRQRIFVEDVVDECKTMYF 331
           K+ SSI  II  R    ++ E+ +YGND L ++L A +  +  +++ ++++++ECKT +F
Sbjct: 324 KVNSSIKRIIDAR----LTSESKDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFF 383

Query: 332 AGQETTGVLLSWAAFLLATHVHWQEEARKEVLDMFGNEN-PTLEGIPKLKIMSMIIKETL 391
           AG ETT  LL+W+  LL+ H  WQE+ R+EV +  G +  P  E   KLK+M+ +  E+L
Sbjct: 384 AGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESL 443

Query: 392 RLYPPAQVMSRMVKKEVRLGKLVLPANIQVSIPTIAVHHDSAIWGEDAHEFKPERFSEG- 451
           RLY P   + R+  ++++LG L +P    + +P   +H D A+WG DA +F P RF+ G 
Sbjct: 444 RLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGL 503

Query: 452 -KAEDIPSGAYIPFGLGPRNCVGNMLAINETKITLSMILQRFSFTISPGHVHAPYQLLTN 511
            +A + P+ A + F +GPR C+G   AI E K  L+MILQRF   +S  + HAP   LT 
Sbjct: 504 SRAANHPN-ALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTL 563

Query: 512 CPQHGVRVKLQSLTG 520
            PQ+ + V L+ + G
Sbjct: 564 QPQYDLPVILEPIDG 572