Spg026022.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg026022.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold7: 1640009 .. 1641612 (-)
Sequence length	492
RNA-Seq Expression	Spg026022.1
Synteny	Spg026022.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACTCACCACCTTGGTGTACAAGGAAGAACAAAGGTGGAGCCTTGGCCATTAAGGCTCTCATTGCCTATATACATGTGTGTTTGTCTCTTGAAGAGAGCAAGAAAAAGAGAGTGATCTTTTCTTCCTTTGTAGTGTGGGGGAGACAGATAAGAGTGTTTCTCTTCTCGGGTTGAGGGTTTTTTCTTGTAGTTTATCCAAAGCTATCTGATTATGATACTTGGTAGTCAATAGCCATTGTTGGGCTATTGAGGGATAGGTTGAGGTGTTGTTCTTGTAGGCTTTTTGTAATCTCTCTAGAGTTTTAGTGGAACCTGCATCAGTAGCAGAGAACTAGACGTAGCCCACATTGGGTGAACCAGTATAAAACTCGGTGTCAATTTCTCTTCCCTTCTCTTTTATTCATTATTGTTCTTGTTGCTTAGAACTTAGATTGGTTTATCTATTCTTCTAAGCTCTTGGTTTCAAATTATTTATTTCGCATTATATTTGGAACCATAACCTGGTCTGGGAATTGCGATAAATATATATTGTCTGAATTTGGTTGATCGAATTTAGATTGGACTATCTTATTGCCTAACACATTCATCTTGGAATCAGATTCTATGGCCATTCTGAATACACCAATCCGACCAACCAGGAGTGAGGATGAAATCATATGAAATTATGATTCTAAAGGAAAGTTTTATGTTAAAAGTGTCTATCGTCTGGGATGTCATCTAAGACAAAGTCTTCAGGCTTCATCTTCCACCTATAAGGCTCAAGAGTTGATGTGGAAATGTTTTTGGAAGGCTAATCTCCCTCAAAAGATAAAAATCTGCGGATGGAGGATATATAATGACATTCTTCCTACTTTATCGAATATTATTAAGAGAGGAATGGATGTTAACTCCATTTGTTATCTGTGCAGGGAGAAGCCGGAATGTCTTAGGCACCTCTTTTGGGAATGTAAATTCACAAAAGGAGTGTGGTATAAATATTTCCCTTATGCTCATTCTGTTCTTTCTAATGGCAGGTGTGGATGGTCTGTTCCTGACTGCTGTGGGGGGTTTTGGAAGGGGAACAACAAGGACGCGTTGGATATTCAAAGTCTACAAAAAAAGCCTTATTATTTGCTGGAAAATATGGACTTATCGGAACTCCATCAGACACGACAAACAGAAGTCCAAATCAGAAATTTTGTACCAGCAGATTGACAAATCTATAGATGAGCTCGTGGACAAAGAAGGCACGCATCTTAGATTGACGAACCAACAACGGTCCTGCATCATTGTTAGCCCGATTACTCCTCAGTGGAAGCCAATCCCAGAAGGCGTCTGGAAGCTGAATTGCGGCGCCAGTTGGAACAAAGAGACGCTGCGAGGTGGGGTAGGATGGCTGCTACGCGACTGGAAAGGCACGATTCAAATGGCGGGTTATAAATCTATCGAGCGTGGCTGGAAAATTCCTTGGTTGGAGTCGATGGCGATTTGTGAAGGGTTGCGGGCGCTTCTCTCGGATCCTCCTTACCCGATGTGCGTCGAGAGTGACTCCTTACAAGTGGTACGCCTGATAAATGAAGAAGATGAGGATTTTACTGAACTGGCTCTTTTCATTAAATAA

mRNA sequence

ATGAAACTCACCACCTTGGTGTACAAGGAAGAACAAAGACACGACAAACAGAAGTCCAAATCAGAAATTTTGTACCAGCAGATTGACAAATCTATAGATGAGCTCGTGGACAAAGAAGGCACGCATCTTAGATTGACGAACCAACAACGGTCCTGCATCATTGTTAGCCCGATTACTCCTCAGTGGAAGCCAATCCCAGAAGGCGTCTGGAAGCTGAATTGCGGCGCCAGTTGGAACAAAGAGACGCTGCGAGGTGGGGTAGGATGGCTGCTACGCGACTGGAAAGGCACGATTCAAATGGCGGGTTATAAATCTATCGAGCGTGGCTGGAAAATTCCTTGGTTGGAGTCGATGGCGATTTGTGAAGGGTTGCGGGCGCTTCTCTCGGATCCTCCTTACCCGATGTGCGTCGAGAGTGACTCCTTACAAGTGGTACGCCTGATAAATGAAGAAGATGAGGATTTTACTGAACTGGCTCTTTTCATTAAATAA

Coding sequence (CDS)

ATGAAACTCACCACCTTGGTGTACAAGGAAGAACAAAGACACGACAAACAGAAGTCCAAATCAGAAATTTTGTACCAGCAGATTGACAAATCTATAGATGAGCTCGTGGACAAAGAAGGCACGCATCTTAGATTGACGAACCAACAACGGTCCTGCATCATTGTTAGCCCGATTACTCCTCAGTGGAAGCCAATCCCAGAAGGCGTCTGGAAGCTGAATTGCGGCGCCAGTTGGAACAAAGAGACGCTGCGAGGTGGGGTAGGATGGCTGCTACGCGACTGGAAAGGCACGATTCAAATGGCGGGTTATAAATCTATCGAGCGTGGCTGGAAAATTCCTTGGTTGGAGTCGATGGCGATTTGTGAAGGGTTGCGGGCGCTTCTCTCGGATCCTCCTTACCCGATGTGCGTCGAGAGTGACTCCTTACAAGTGGTACGCCTGATAAATGAAGAAGATGAGGATTTTACTGAACTGGCTCTTTTCATTAAATAA

Protein sequence

MKLTTLVYKEEQRHDKQKSKSEILYQQIDKSIDELVDKEGTHLRLTNQQRSCIIVSPITPQWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAICEGLRALLSDPPYPMCVESDSLQVVRLINEEDEDFTELALFIK

Homology

[Close]

BLAST of Spg026022.1 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 95.5 bits (236), Expect = 4.5e-16
Identity = 44/98 (44.90%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           QWKP     WKLN  A+W  +T  GG+GW+LRD KG +  A  + I     I +LE MAI
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLINEEDEDFTEL 159
           CEGLRA+  +   P+ +ESDSL+ + L++ + +D TE+
Sbjct: 138 CEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of Spg026022.1 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 48/142 (33.80%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 17  QKSKSEILYQQIDKSIDELVDKEGTHLRLTNQQRSCIIVSPITPQWKPIPEGVWKLNCGA 76
           + S    + QQ+ K + E   +  T L + ++  +  +      +W+P P  +W LN  A
Sbjct: 32  EHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKL------KWEPPPMHIWTLNADA 91

Query: 77  SWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAICEGLRALLS-DPPYPM 136
           SW+  T RGG+GW++R W G I +AG + +E    +  LE+ AI EGLR L +     P+
Sbjct: 92  SWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRPL 151

Query: 137 CVESDSLQVVRLINEEDEDFTE 158
            +E+DS +V  L+N + ED T+
Sbjct: 152 HIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of Spg026022.1 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-13
Identity = 39/89 (43.82%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T   G+GW+LRD KG +   G + I     I +LE MAI
Sbjct: 254 RWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAI 313

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLIN 150
           CEGLRA+  +   P+ +ESDSL+ + L++
Sbjct: 314 CEGLRAIRQEHCRPIHLESDSLEAIHLLH 342

[Close]

BLAST of Spg026022.1 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-13
Identity = 39/89 (43.82%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T   G+GW+LRD KG +   G + I     I +LE MAI
Sbjct: 219 RWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAI 278

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLIN 150
           CEGLRA+  +   P+ +ESDSL+ + L++
Sbjct: 279 CEGLRAIRQEHCRPIHLESDSLEAIHLLH 307

[Close]

BLAST of Spg026022.1 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-13
Identity = 44/106 (41.51%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T  GG+GW+LRD KG +  A  + I     I +LE MAI
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 121 CEGLRALLSDPPYPM----C----VESDSLQVVRLINEEDEDFTEL 159
           CEGLRA+  +   P+    C    +ESDSL+ + L++ + +D TE+
Sbjct: 138 CEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of Spg026022.1 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.2e-16
Identity = 44/98 (44.90%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           QWKP     WKLN  A+W  +T  GG+GW+LRD KG +  A  + I     I +LE MAI
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLINEEDEDFTEL 159
           CEGLRA+  +   P+ +ESDSL+ + L++ + +D TE+
Sbjct: 138 CEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of Spg026022.1 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 5.9e-14
Identity = 48/142 (33.80%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 17  QKSKSEILYQQIDKSIDELVDKEGTHLRLTNQQRSCIIVSPITPQWKPIPEGVWKLNCGA 76
           + S    + QQ+ K + E   +  T L + ++  +  +      +W+P P  +W LN  A
Sbjct: 32  EHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKL------KWEPPPMHIWTLNADA 91

Query: 77  SWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAICEGLRALLS-DPPYPM 136
           SW+  T RGG+GW++R W G I +AG + +E    +  LE+ AI EGLR L +     P+
Sbjct: 92  SWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRPL 151

Query: 137 CVESDSLQVVRLINEEDEDFTE 158
            +E+DS +V  L+N + ED T+
Sbjct: 152 HIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of Spg026022.1 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 44/106 (41.51%), Postives = 63/106 (59.43%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T  GG+GW+LRD KG +  A  + I     I +LE MAI
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 121 CEGLRALLSDPPYPM----C----VESDSLQVVRLINEEDEDFTEL 159
           CEGLRA+  +   P+    C    +ESDSL+ + L++ + +D TE+
Sbjct: 138 CEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of Spg026022.1 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 39/89 (43.82%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T   G+GW+LRD KG +   G + I     I +LE MAI
Sbjct: 254 RWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAI 313

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLIN 150
           CEGLRA+  +   P+ +ESDSL+ + L++
Sbjct: 314 CEGLRAIRQEHCRPIHLESDSLEAIHLLH 342

[Close]

BLAST of Spg026022.1 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 39/89 (43.82%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 61  QWKPIPEGVWKLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAI 120
           +WKP     WKLN  A+W  +T   G+GW+LRD KG +   G + I     I +LE MAI
Sbjct: 219 RWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAI 278

Query: 121 CEGLRALLSDPPYPMCVESDSLQVVRLIN 150
           CEGLRA+  +   P+ +ESDSL+ + L++
Sbjct: 279 CEGLRAIRQEHCRPIHLESDSLEAIHLLH 307

[Close]

BLAST of Spg026022.1 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.1e-07
Identity = 32/142 (22.54%), Postives = 72/142 (50.70%), Query Frame = 0


Query: 18  KSKSEILYQQIDKSIDELVDKEGTHL---RLTNQQRSCIIVSPIT----PQWKPIPEGVW 77
           K+++E++++  + +  E++ +    L   R+  +  SC     +      +W+P P    
Sbjct: 369 KNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGRWRPPPHQWV 428

Query: 78  KLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAICEGLRALLSD 137
           K N  A+WN++  R G+GW+LR+ KG ++  G +++ +   +   E  A+   + +L   
Sbjct: 429 KCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRF 488

Query: 138 PPYPMCVESDSLQVVRLINEED 153
               +  ESDS  ++ ++N ++
Sbjct: 489 QYNYVIFESDSQVLIEILNNDE 510

[Close]

BLAST of Spg026022.1 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 33/142 (23.24%), Postives = 65/142 (45.77%), Query Frame = 0


Query: 18  KSKSEILYQQIDKSIDELV-------DKEGTHLRLTNQQRSCIIVSPITPQWKPIPEGVW 77
           KS++E++++  +    E++       ++  T   L  +     +   ++ QWK  P    
Sbjct: 86  KSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKASGPQVERNLSVQWKAPPYQWV 145

Query: 78  KLNCGASWNKETLRGGVGWLLRDWKGTIQMAGYKSIERGWKIPWLESMAICEGLRALLSD 137
           K N  A+W  E  R G+GW+LR+  G +   G +++ R   +   E  A+   +  +   
Sbjct: 146 KCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRF 205

Query: 138 PPYPMCVESDSLQVVRLINEED 153
               +  ESD+  +V L+N +D
Sbjct: 206 NYKRIIFESDAQALVNLLNSDD 227

The following BLAST results are available for this feature:

BLAST of Spg026022.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	4.5e-16	44.90	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022155262.1	1.2e-13	33.80	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022154990.1	2.7e-13	43.82	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	2.7e-13	43.82	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]
XP_022156777.1	2.7e-13	41.51	uncharacterized protein LOC111023608 [Momordica charantia]	[more]

BLAST of Spg026022.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg026022.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	2.2e-16	44.90	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DNV9	5.9e-14	33.80	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DSV1	1.3e-13	41.51	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DL64	1.3e-13	43.82	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	1.3e-13	43.82	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Spg026022.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	3.1e-07	22.54	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	3.4e-06	23.24	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 73..160 e-value: 1.6E-10 score: 40.9
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 57..159
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 72..163 e-value: 4.65127E-12 score: 57.7092
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 69..150

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg026022	Spg026022	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg026022.1-cds	Spg026022.1-cds-scaffold7:1640009..1640462	CDS
Spg026022.1-cds	Spg026022.1-cds-scaffold7:1641575..1641612	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg026022.1	Spg026022.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity