Spg025855.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg025855.1 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.2e-299
Identity = 528/556 (94.96%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSG
Sbjct: 51  ATNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
            AYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 NAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWHKLLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK
Sbjct: 351 IVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           D+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAETAS 606

BLAST of Spg025855.1 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.8e-299
Identity = 528/556 (94.96%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSG
Sbjct: 51  ANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
           KAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 KAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWHKLLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK
Sbjct: 351 IVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           D+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAEAAS 606

BLAST of Spg025855.1 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.7e-299
Identity = 527/556 (94.78%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSG
Sbjct: 51  ANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
           KAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 KAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWH+LLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK
Sbjct: 351 IVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           D+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+ PN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAETAS 606

BLAST of Spg025855.1 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1034.6 bits (2674), Expect = 3.0e-298
Identity = 524/556 (94.24%), Postives = 546/556 (98.20%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRDS EDEEHIFRSQLPEVQVPDDITLPEFVLQNAE YA+NVAFVEAVSG
Sbjct: 51  ANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
           KAYTYREVVRDT+RFAKALSSLRL+KGQ+V+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 KAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWHKLLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG+IGK
Sbjct: 351 IVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAETAS 606

BLAST of Spg025855.1 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 1029.6 bits (2661), Expect = 9.8e-297
Identity = 524/550 (95.27%), Postives = 541/550 (98.36%), Query Frame = 0

Query: 9   MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68
           MATC RD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 69  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128
           EVVRDTNRF+KAL SLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 129 KKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKE 188
           KKQVEVA+AKLV+TNSANFEKVKELKLPVI+LGEELIE SMNWHKLLEAADRAGNN VKE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 189 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHI 248
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 249 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 308
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 309 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAR 368
           LSGLKLQAIMTAAAPLAPELQ+AFE+KFPGVDVQEAYGLTEHSCITLNYGSIGKENL A+
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLAAK 360

Query: 369 KNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWM 428
           KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYYKNEEETSRTIDNKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420

Query: 429 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 488
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 489 EIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 548
           EIPAASVV+APNSKETE+EIIK+VASNVA+YKKVRLVHFVD IPKSPSGKVMRRL+KEKM
Sbjct: 481 EIPAASVVMAPNSKETEEEIIKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKM 540

Query: 549 IEKIRAESSS 559
           IEKIRAES+S
Sbjct: 541 IEKIRAESAS 550

BLAST of Spg025855.1 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 8.5e-227
Identity = 394/532 (74.06%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 18  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 77
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 78  AKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 137
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 138 KLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 197
           + ++T++ N+EKVK L LPVIVLGEE IE ++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 198 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCA 257
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 258 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 317
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 318 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 377
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    A++N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 378 NLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 437
           NLEVKFIDP +GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 438 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 497
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VVI
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 498 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 550
            P + E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Spg025855.1 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-199
Identity = 347/539 (64.38%), Postives = 438/539 (81.26%), Query Frame = 0

Query: 18  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVS-GKAYTYREVVRDTNR 77
           E++EH+FRS+ P V VPD +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 78  FAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 137
           FA+AL S+ +RKG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 138 AKLVVTNSANFEKVKELKLPVIVLGE-ELIEDSMNWHKLLEAADRAGNNFVKED-IKQTD 197
           AKLVV N   F+KVK+  +PVI +G+ E +  +++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 198 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGI 257
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 258 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 317
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 318 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 377
           +++MTAAAPLAP+L  AF++KFPGV V+EAYGLTEHSCITL + +       A+K++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 378 ILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 437
           ILPNLEVKF+DP +GRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 438 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 497
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 498 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 554
           VV    ++E E+EI+ YVA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of Spg025855.1 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.3e-113
Identity = 210/534 (39.33%), Postives = 330/534 (61.80%), Query Frame = 0

Query: 20  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 79
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 80  ALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 139
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 140 VVTNSANFEKVKEL---KLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKEDIKQTDLC 199
           ++T +   +KVK+    K   I+  ++  +D +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 200 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 259
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 260 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 319
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 320 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 379
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    +    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 380 ILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 439
           ++ N E+K +DP +  SLP+N  GE+C+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 440 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 499
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 500 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of Spg025855.1 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.2e-113
Identity = 211/534 (39.51%), Postives = 330/534 (61.80%), Query Frame = 0

Query: 20  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 79
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 80  ALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 139
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 128

Query: 140 VVTNSANFEKVKEL---KLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKEDIKQTDLC 199
           ++T +   +KVK+    K   I+  ++  +D +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---IDSDDVV 188

Query: 200 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 259
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 260 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 319
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 320 QAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGR 379
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    +    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 380 ILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 439
           ++ N E+K +DP +  SLP+N  GE+C+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 440 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 499
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 500 VVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           VV       TE+EI ++V+  V  YK++  V FVD IPKSPSGK++R+ ++ K+
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKI 537

BLAST of Spg025855.1 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.4e-112
Identity = 207/547 (37.84%), Postives = 324/547 (59.23%), Query Frame = 0

Query: 9   MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68
           MA  +    + ++ IFRS+LP++ +P ++ L  +  +N   ++     +   + + +TY 
Sbjct: 1   MAAAVAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYA 60

Query: 69  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128
           +V   + R    LS L +++G  ++++LPN  E+    LG    G + +  NP    +E+
Sbjct: 61  DVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEV 120

Query: 129 KKQVEVADAKLVVTNSANFEKVKELKLP-----VIVLGEELIEDSMNWHKLLEAADRAGN 188
            KQ + ++AKL++T     +KVK+         + +      +D+ N     E      N
Sbjct: 121 IKQAKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADEN 180

Query: 189 NFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLG 248
              K +I    + ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M      L 
Sbjct: 181 EMPKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLC 240

Query: 249 LIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKN 308
           ++P FHIY +  +    LR    +++M +F++  F+  +   +VT  P VPPI+LA+ K+
Sbjct: 241 VLPLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKS 300

Query: 309 PIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIG 368
            +V+ +DLS ++   +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  
Sbjct: 301 TVVDNYDLSSVR--TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFA 360

Query: 369 KENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRT 428
           KE    +    G ++ N E+K +DP +G SLP+N PGE+C+R   +M+GY  + E T+RT
Sbjct: 361 KEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATART 420

Query: 429 IDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVP 488
           ID +GW+HTGDIGYIDDD ++FIVDR+KELIKYKGFQVAPAELEA+LLTHP I DAAVVP
Sbjct: 421 IDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVP 480

Query: 489 LPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMR 548
           + DE AGE+P A VV +     TEDEI ++++  V  YK++  V FV+ IPK+PSGK++R
Sbjct: 481 MKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILR 540

BLAST of Spg025855.1 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.3e-299
Identity = 528/556 (94.96%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSG
Sbjct: 51  ANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
           KAYTYREVVRDT+RFAKALSSLRL+KGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 KAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWHKLLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLSGLKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK
Sbjct: 351 IVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           D+KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAEAAS 606

BLAST of Spg025855.1 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1034.6 bits (2674), Expect = 1.5e-298
Identity = 524/556 (94.24%), Postives = 546/556 (98.20%), Query Frame = 0

Query: 3   AANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSG 62
           A NNS MATCIRDS EDEEHIFRSQLPEVQVPDDITLPEFVLQNAE YA+NVAFVEAVSG
Sbjct: 51  ANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSG 110

Query: 63  KAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPA 122
           KAYTYREVVRDT+RFAKALSSLRL+KGQ+V+VVLPNVAEYAIVALGIMAAGGVFSGVNPA
Sbjct: 111 KAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPA 170

Query: 123 AHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAG 182
           AHISEIKKQVEVADAKLVVTNS++FEKVKELKLPVIV+GEELIE SMNWHKLLEAADRAG
Sbjct: 171 AHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAG 230

Query: 183 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGL 242
           NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEM GKVTTLGL
Sbjct: 231 NNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGL 290

Query: 243 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 302
           IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP
Sbjct: 291 IPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNP 350

Query: 303 IVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGK 362
           IV+EFDLS LKLQAIMTAAAPLAPELQT+FEKKFPGVDVQEAYGLTEHSCITLNYG+IGK
Sbjct: 351 IVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGK 410

Query: 363 ENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTI 422
           +NL A+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSRTI
Sbjct: 411 DNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTI 470

Query: 423 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 482
           DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL
Sbjct: 471 DNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPL 530

Query: 483 PDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRR 542
           PD+EAGEIPAAS+V+APN+KETEDEIIKYVASNVAHYKKVRLVHFV+TIPKSPSGKVMRR
Sbjct: 531 PDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRR 590

Query: 543 LIKEKMIEKIRAESSS 559
           LIKEKMIEKIRAE++S
Sbjct: 591 LIKEKMIEKIRAETAS 606

BLAST of Spg025855.1 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.4e-296
Identity = 525/558 (94.09%), Postives = 543/558 (97.31%), Query Frame = 0

Query: 1   MPAANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV 60
           MP AN S M T IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV
Sbjct: 52  MPVANISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV 111

Query: 61  SGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVN 120
           SGKAYTYREVVRDT RFAKALSSLRLRKGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVN
Sbjct: 112 SGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVN 171

Query: 121 PAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADR 180
           PAAHISEIKKQVE ADAKLVVTNS NFEKVKELKLPVIVLGEELIE SMNWHKLLEAADR
Sbjct: 172 PAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADR 231

Query: 181 AGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTL 240
           AGNN VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTL
Sbjct: 232 AGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTL 291

Query: 241 GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK 300
           GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK
Sbjct: 292 GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK 351

Query: 301 NPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI 360
           NPIVEEFDLS LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI
Sbjct: 352 NPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI 411

Query: 361 GKENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSR 420
           GKENLTA+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSR
Sbjct: 412 GKENLTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSR 471

Query: 421 TIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV 480
           TIDN+GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV
Sbjct: 472 TIDNRGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV 531

Query: 481 PLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVM 540
           PLPDEEAGEIPAASVVIAPNSKETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKVM
Sbjct: 532 PLPDEEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVM 591

Query: 541 RRLIKEKMIEKIRAESSS 559
           RRLIKEKMI+KIRA+++S
Sbjct: 592 RRLIKEKMIDKIRAKTAS 609

BLAST of Spg025855.1 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.4e-296
Identity = 525/558 (94.09%), Postives = 543/558 (97.31%), Query Frame = 0

Query: 1   MPAANNSDMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV 60
           MP AN S M T IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV
Sbjct: 1   MPVANISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAV 60

Query: 61  SGKAYTYREVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVN 120
           SGKAYTYREVVRDT RFAKALSSLRLRKGQVV+VVLPNVAEYAIVALGIMAAGGVFSGVN
Sbjct: 61  SGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVN 120

Query: 121 PAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADR 180
           PAAHISEIKKQVE ADAKLVVTNS NFEKVKELKLPVIVLGEELIE SMNWHKLLEAADR
Sbjct: 121 PAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADR 180

Query: 181 AGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTL 240
           AGNN VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E EGKVTTL
Sbjct: 181 AGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTL 240

Query: 241 GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK 300
           GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK
Sbjct: 241 GLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVK 300

Query: 301 NPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI 360
           NPIVEEFDLS LKLQAIMTAAAPLAPE+QTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI
Sbjct: 301 NPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSI 360

Query: 361 GKENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSR 420
           GKENLTA+KNTVGRILPNLEVKFIDP SGRSLPKNTPGE+CVRSQCVMQGYY NEEETSR
Sbjct: 361 GKENLTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSR 420

Query: 421 TIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV 480
           TIDN+GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV
Sbjct: 421 TIDNRGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVV 480

Query: 481 PLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVM 540
           PLPDEEAGEIPAASVVIAPNSKETE+EII+YVASNVA+YKKVR+VHFVD+IPKSPSGKVM
Sbjct: 481 PLPDEEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVM 540

Query: 541 RRLIKEKMIEKIRAESSS 559
           RRLIKEKMI+KIRA+++S
Sbjct: 541 RRLIKEKMIDKIRAKTAS 558

BLAST of Spg025855.1 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 508/550 (92.36%), Postives = 537/550 (97.64%), Query Frame = 0

Query: 9   MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68
           MATC +DSV+DEEHIF SQLPEVQVP DITLPEFVLQNAESYAENVAFVEA+SGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 69  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128
           EV+RDTNRF+KAL SLRL+KG VV+VVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 129 KKQVEVADAKLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKE 188
           KKQVEVA+AKLVVTN+ANFEKV+ELKLPVI+L EEL+E +MNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 189 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHI 248
           +IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+CSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 249 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 308
           YGITGICC+TLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 309 LSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAR 368
           LS LKLQAIMTAAAPLAPELQTAFE+KFPGVDVQEAYGLTEH CITLNYGSIGKENL+A+
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 369 KNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWM 428
           KNTVGRILPNLEVKFIDP +GRSLPKNTPGE+CVRSQCVMQGYYKNEEETSRTID KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 429 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 488
           HTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 489 EIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 548
           EIPAASVV+APNSKETE++IIK+VASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 549 IEKIRAESSS 559
           IEKIRA+ S+
Sbjct: 541 IEKIRADESA 550

BLAST of Spg025855.1 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 787.7 bits (2033), Expect = 6.0e-228
Identity = 394/532 (74.06%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 18  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 77
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 78  AKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 137
           AKAL+SL LRKGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 138 KLVVTNSANFEKVKELKLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 197
           + ++T++ N+EKVK L LPVIVLGEE IE ++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 198 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCA 257
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 258 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 317
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 318 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 377
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    A++N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 378 NLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 437
           NLEVKFIDP +GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 438 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 497
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VVI
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 498 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 550
            P + E E++I+ +VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of Spg025855.1 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 396.4 bits (1017), Expect = 3.9e-110
Identity = 202/532 (37.97%), Postives = 327/532 (61.47%), Query Frame = 0

Query: 23  IFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 82
           IFRS+LP++ +P+ + L +++ +N   +A     +   +G+ YTY +V   + + A  L 
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 83  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 142
           +L +++  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 143 NSANFEKVKELK----LPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKEDIKQTDLCAL 202
            S   +K+K L+    L V    + + E+ + + +L ++ +   ++ + E I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 203 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--EGKVTTLGLIPFFHIYGITGICC 262
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 263 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQA 322
            +LR    +++M +F++   +  +   +VT A +VPPI+LA+ K+P  E++DLS +++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 323 IMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRIL 382
           + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    +    G ++
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 383 PNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYI 442
            N E+K +DP +G SLP+N PGE+C+R   +M+GY  +   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 443 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVV 502
           DDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A VV
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 503 IAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
            + +S  +EDEI ++V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of Spg025855.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 383.3 bits (983), Expect = 3.4e-106
Identity = 196/536 (36.57%), Postives = 319/536 (59.51%), Query Frame = 0

Query: 23  IFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 82
           IFRS+LP++ +P+ ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 83  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 142
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 143 NSANFEKVKELK----LPVIVLGEE----LIEDSMNWHKLLEAADRAGNNFVKEDIKQTD 202
            +   +K+K L+    + ++ + +     + E  + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 203 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--EGKVTTLGLIPFFHIYGIT 262
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 263 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGL 322
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   E++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 323 KLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTV 382
           ++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    +    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 383 GRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGD 442
           G ++ N E+K +DP +G SL +N PGE+C+R   +M+GY  N   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 443 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPA 502
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + +E AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 503 ASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           A VV + +S+ +ED++ ++V+  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Spg025855.1 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 380.2 bits (975), Expect = 2.9e-105
Identity = 204/531 (38.42%), Postives = 321/531 (60.45%), Query Frame = 0

Query: 23  IFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALS 82
           IFRS+LP++ +P+ + L  +  +   S ++    +   +GK+YTY E      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 83  SLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 142
            L +RKG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 143 NSANFEKVKEL--KLPVIVLGEELIEDSMNWHKLLEAADRAGNNFVKE-DIKQTDLCALP 202
           +S   +K+K L   L +I   E   E+ + +  L+   D   N F +  DI   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 203 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCA 262
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +   
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 263 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 322
           +LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS ++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 323 MTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTARKNTVGRILP 382
           ++ AAPL  ELQ +  ++ P   + + YG+TE   +        KE +  +  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 383 NLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 442
           N E+K +   +  SL  N PGE+C+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 443 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVI 502
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 503 APNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 549
           +  +  TE+++ +YVA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Spg025855.1 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 375.2 bits (962), Expect = 9.3e-104
Identity = 198/553 (35.80%), Postives = 327/553 (59.13%), Query Frame = 0

Query: 15  DSVEDEEH--IFRSQLPEVQVPDDITLPEFVLQ----NAESYAENVAFVEAVSGKAYTYR 74
           D  E+  H  IFRS+LP++ +P+ + L ++V Q    + +  +     ++  +G+  TY 
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 75  EVVRDTNRFAKALSSLRLRKGQVVLVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 134
           +V  +  R A  +  L +R G VV+++LPN  E+A+  L +   G V +  NP     EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 135 KKQVEVADAKLVVTNSANFEKVKELKLPVIVL------GEELIEDS-----MNWHKLLEA 194
            KQ + + AK+++T     +K+  LK   +++      G+  +  S     +++ +L +A
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 197

Query: 195 ADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEME--G 254
            +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +    
Sbjct: 198 DE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTA 257

Query: 255 KVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 314
               L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP++
Sbjct: 258 NDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVV 317

Query: 315 LALVKNPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFEKKFPGVDVQEAYGLTEHSCITL 374
           LA +K+P  E +DLS +++  +++ AA L  EL+ A   KFP     + YG+TE   +  
Sbjct: 318 LAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAK 377

Query: 375 NYGSIGKENLTARKNTVGRILPNLEVKFIDPGSGRSLPKNTPGEVCVRSQCVMQGYYKNE 434
           +  +  K     +    G ++ N E+K +D  +G SLP+N  GE+CVR   +M+GY  + 
Sbjct: 378 SL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDP 437

Query: 435 EETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIE 494
           E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI+
Sbjct: 438 EATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSID 497

Query: 495 DAAVVPLPDEEAGEIPAASVVIAPNSKETEDEIIKYVASNVAHYKKVRLVHFVDTIPKSP 549
           DAAVV + DE A E+P A V  +  S+ TED++  YV   V HYK++++V F++ IPK+ 
Sbjct: 498 DAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAV 557