Spg022623.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg022623.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold2: 3391246 .. 3394463 (-)
Sequence length1554
RNA-Seq ExpressionSpg022623.1
SyntenySpg022623.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTATATGGAACCTTCATCTTCATCTTCACCTCCCTTCCTCTCTCTCCTCTGTAAGCAGAGTAATAATGGAGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTCGAAAGACGAGAGAATAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGGTGAGTTGGTTTTCTGTTTGTTTCTTGGGAAAGTTACATCTTCTTAGTAATCTGTCCGATTTTAGAAGACAATAATTTGTTATTGCTTAGCATCTTAGAGCGAACTAGGAGACTGTGAATTTGTTGGTTTTGGAATCTGCTACTTTCATTCCCTTATATAACAGATATTCAATCATGGTTTATTGAGAATGATGCACTTTGTTTCTAAAGCTAGTCATCTGACTTCAAATGGTCTTGATTGGAATACATGTCTAGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACAGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTCGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGCCTTGAAACTCGGGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGGTAAAAATCACTTTTACTGTTTCTGCTTTTATCATAATCACAAAGATGCAGTTCTCTCACAATGTTCAAAAAGATTTTCAACAGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGGTATAAACTAGAGACATCAGTTCTTCAGGAGCAAGTTCCTTTTCTTTCTTTTTTATCTAACCACTTCTAGCTTTACCAACACATTTCTTTACCAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGGTAAATTTGCCACTATTTCTCATAAGAATCTCCTTTTTCTCTAACGATAACTTAAAAATGATCACTTTCATGTCTTTCTCCATCTTGCTTGAATTGGGAAAGTGAGAACCTTGCAAATTTTTTTTTATATGAATGGATATAATTCAAATTTAACTAAGATTTCTGTCCTTCATTTCATAAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTCTAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACAAGAGGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTTCAGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGTTCTTACTTCATAATTATTTACAATCGTATATTTTTCTACTCTGTATGCATTTCATTTCATTTGACTCCAGCTACCTTCCAATTGGAAATTATAGAGTTGGAATCGAATGATTGTAAGATACACTGCCTTCTGCTATTTACGACTGTTGGGAGACCAAGTTCTAATTCGAAGATTGGACTCTTTTGCTGTAATTAGCTCTTGTTTCTTTTGGAAACCATGATTGTTTGAACCAACTTGCATGCACTAATCTTACGAGATAACACATAAAAACTCATGAGATATCTGAAATCTTCAATAGGTGGCCATTATAGTTGAATTCATTACCATATGCCAATGAACGTCATAGTTTACACTGTATCTTTTTGACTGGTAGGCAACCTGTTGTGGTTGTGATTAGCCCTGCCAAGTTGTCTAGATGACACTTTCTAGTCGAGTACTAAGACGTGAAATGGAAATGAAAGAGGAACTCTATGCCTTCCAAAAAGTGCAATTTATTTCAAGTTAGGTCATAAAAAATCAAACATAATCTTTTTGAAACTCTAGTTTCAGCCACAAGAAAAGGGCAAAATTTTTACTGAATATTATATTTTTTAAGGTAAACCTTGATAAATAGTATGCAATAAAATGAATTTGACCTGTCTGGCTTTCTCCTTTGGACATGAGTAAATTGCTAAATCTCTCTGTTCTTCATTTTGTTAATATTAGACTTGGTATTATATTTGTACGGTCGCAAATAACTTGATCCTTTTGTCTGTACAATACTTTTCTATTCCTTGTGATCTAACCAGACACATTTACCTGCATAAAGTATTGGTATTTTGAAGAATCTGTTTGTATGTGCTTAAATAAAATGCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCTTTTGTCTTGCATGTTGGGGGAAAGGTAAATATGATAATCATCTCCACCCTTCATGCTCACTATTTTACTTTATGTCCTATATTATTACTAATCCATTTTTTTAACATTTTTCTCTTGAAAATGATCCTCAATAGGGGCTGTGGTTAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATCCGCACCAACTGGCACCAAGAAGTACAACTCTAAACTCAAATCTCAACTTTCCTTGATTCCCTTGAAATTTGATAGTCCTGATGCACTTATTCATCATATTCAGGCAAAGATAGCTACAGAACGAGTTTACAATGCAGAAATTCCAACGAATGTCATCTCATGA

mRNA sequence

ATGGAGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTCGAAAGACGAGAGAATAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACAGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTCGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGCCTTGAAACTCGGGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTCTAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACAAGAGGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTTCAGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCTTTTGTCTTGCATGTTGGGGGAAAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATCCGCACCAACTGGCACCAAGAAGTACAACTCTAAACTCAAATCTCAACTTTCCTTGATTCCCTTGAAATTTGATAGTCCTGATGCACTTATTCATCATATTCAGGCAAAGATAGCTACAGAACGAGTTTACAATGCAGAAATTCCAACGAATGTCATCTCATGA

Coding sequence (CDS)

ATGGAGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTCGAAAGACGAGAGAATAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACAGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTCGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGCCTTGAAACTCGGGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTCTAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACAAGAGGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTTCAGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCTTTTGTCTTGCATGTTGGGGGAAAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATCCGCACCAACTGGCACCAAGAAGTACAACTCTAAACTCAAATCTCAACTTTCCTTGATTCCCTTGAAATTTGATAGTCCTGATGCACTTATTCATCATATTCAGGCAAAGATAGCTACAGAACGAGTTTACAATGCAGAAATTCCAACGAATGTCATCTCATGA

Protein sequence

MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQLSLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS
Homology
BLAST of Spg022623.1 vs. NCBI nr
Match: XP_023531831.1 (protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.4 bits (2079), Expect = 2.8e-229
Identity = 428/517 (82.79%), Postives = 461/517 (89.17%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S LNS L+HISEDEVN KD R+ DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAM +LLL+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMVILLLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD+VPII+VS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+GGK   + ++S     L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIIS----ALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNVI+
Sbjct: 481 AIITIRTNWDE------EAKIAAERVYESVIPTNVIA 494

BLAST of Spg022623.1 vs. NCBI nr
Match: XP_022929082.1 (protein DETOXIFICATION 16-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 802.0 bits (2070), Expect = 3.1e-228
Identity = 426/517 (82.40%), Postives = 459/517 (88.78%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S LNS L+HISEDEVN KD R+ DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALIL+
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILM 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHN  YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNFSYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAV EGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVFEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD+VPII+VS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+GGK   + ++S     L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIIS----ALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNVI+
Sbjct: 481 AIITIRTNWDE------EAKIAAERVYESVIPTNVIA 494

BLAST of Spg022623.1 vs. NCBI nr
Match: KAG6587669.1 (Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 801.2 bits (2068), Expect = 5.2e-228
Identity = 426/517 (82.40%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S LNS L+HISEDEVN KD R+ DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD++PII+VS+F DGLQCVLSGI RG GWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIIPIIAVSTFFDGLQCVLSGIVRGSGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+GGK   + ++S     L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIIS----ALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNV++
Sbjct: 481 AIITIRTNWVE------EAKIAAERVYESVIPTNVVA 494

BLAST of Spg022623.1 vs. NCBI nr
Match: XP_023005095.1 (protein DETOXIFICATION 16-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 425/517 (82.21%), Postives = 457/517 (88.39%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S L S L+HISEDEVN KD  + DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD+VPII+VS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+ GK   + ++      L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIDGKGLWLGII----CALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNVI+
Sbjct: 481 AIITIRTNWDE------EAKIAAERVYESVIPTNVIA 494

BLAST of Spg022623.1 vs. NCBI nr
Match: XP_038880134.1 (protein DETOXIFICATION 16-like isoform X1 [Benincasa hispida])

HSP 1 Score: 780.4 bits (2014), Expect = 9.6e-222
Identity = 417/516 (80.81%), Postives = 454/516 (87.98%), Query Frame = 0

Query: 2   ERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQY 61
           E DQNSSLNS L+H SEDEVNSKDER  + +N R KLIAEEVKKQLWLAGPL+SVSLLQY
Sbjct: 3   EEDQNSSLNSPLIHTSEDEVNSKDERQINYENIRSKLIAEEVKKQLWLAGPLMSVSLLQY 62

Query: 62  CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG 121
           CLQMISIMFVGHLGELPLSGASMAVSFT+VTGFTVL+G+ASALDTFCGQSYGAKQYHMLG
Sbjct: 63  CLQMISIMFVGHLGELPLSGASMAVSFTAVTGFTVLMGLASALDTFCGQSYGAKQYHMLG 122

Query: 122 IHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQC 181
           IHM+RA+ VLLL+SIPLA IWANTGEIL+L+GQDAQIS EAGKYAKCLIPCLFAHGLVQC
Sbjct: 123 IHMERAIIVLLLVSIPLAFIWANTGEILKLLGQDAQISVEAGKYAKCLIPCLFAHGLVQC 182

Query: 182 LNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILIL 241
           LNRFLQTQNVVFPMM+SSGIAA LHI LCWVMVFKAGL TRGAA+ANSISYWINALILIL
Sbjct: 183 LNRFLQTQNVVFPMMMSSGIAALLHIPLCWVMVFKAGLGTRGAAVANSISYWINALILIL 242

Query: 242 YVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE 301
           YVK SSSCSKSWTGFS  AFHN+PYFLKLAIPSTVMVCLELWSFEM+VLLSGLLPNPKLE
Sbjct: 243 YVKLSSSCSKSWTGFSGLAFHNVPYFLKLAIPSTVMVCLELWSFEMMVLLSGLLPNPKLE 302

Query: 302 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVA 361
           TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAA +VM IA+IEGLL+ 
Sbjct: 303 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAACVVMIIAIIEGLLLG 362

Query: 362 TVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYV 421
           TVL+LIR VWGYAYSNEQEVVKYVAD+VP+I+VSSFLDGLQCVLSGIARGCGWQKIGAYV
Sbjct: 363 TVLILIRYVWGYAYSNEQEVVKYVADIVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYV 422

Query: 422 NLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQLS 481
           NLGSYYLVGIPSAVLLAFVL VGGK   + ++      L++ +               L+
Sbjct: 423 NLGSYYLVGIPSAVLLAFVLQVGGKGLWLGII----CALAVQTV-------------SLA 482

Query: 482 LIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           +I ++ +         +AK+ATERVY+A IP N IS
Sbjct: 483 IITIRTNWDQ------EAKMATERVYDAVIPVNAIS 495

BLAST of Spg022623.1 vs. ExPASy Swiss-Prot
Match: Q9FHB6 (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.7e-158
Identity = 278/408 (68.14%), Postives = 339/408 (83.09%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF SVTGF+ L+
Sbjct: 23  VKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSFLM 82

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+  I
Sbjct: 83  GTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQNKSI 142

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAG 218
           +  AG YAK +IP +FA+GL+QC NRFLQ QN VFP++  SG+  SLH+LLCWV+VFK+G
Sbjct: 143 ATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVFKSG 202

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  +GAALANSISYW+N ++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PS +MV
Sbjct: 203 LGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSALMV 262

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLE+WSFE++VLLSGLLPNP LETSVLSI LNT  T+WMIP GLS AASTR+SNELGAG 
Sbjct: 263 CLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELGAGN 322

Query: 339 PAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFL 398
           P  AKLA  +V+ IAV E +++ +VL+LIRN+WG AYS+E EVV YVA ++PI+++ +FL
Sbjct: 323 PKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALGNFL 382

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGK 447
           D LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  HVGG+
Sbjct: 383 DSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGR 430

BLAST of Spg022623.1 vs. ExPASy Swiss-Prot
Match: Q9C9U1 (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.1e-151
Identity = 280/445 (62.92%), Postives = 339/445 (76.18%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+ PLI VSLLQY LQ+IS+MFVGHLG LPLS AS+A SF SVTGFT LL
Sbjct: 21  VKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFLL 80

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASAL+T CGQ+YGAK Y  LGI MQRAMFVLL+LS+PL++IWANT +IL LV QD  I
Sbjct: 81  GTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKSI 140

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAG 218
           ++ AG YAK +IP LFA+GL+QC+NRFLQ QN VFP+ V SGI   LH+LLCW+ V K G
Sbjct: 141 ASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTG 200

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  RGAALA S+SYW N ++L  YVKFS SCS SWTGFS +AF  +  F K+A PS VMV
Sbjct: 201 LGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMV 260

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLELWSFE++VL SGLLPNP LETSVLSI LNT LT+W I +GL  AAS RVSNELGAG 
Sbjct: 261 CLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGN 320

Query: 339 PAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFL 398
           P  AKLA  +++ IAV EG++V TVL+ IR + G+A+S++ +++ Y A ++PI++  +FL
Sbjct: 321 PQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFL 380

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKA---SSVHLLSK 458
           DGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+      V  LS 
Sbjct: 381 DGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRGLWLGIVTALSV 440

Query: 459 HFLLLSLPSAPTGTKKYNSKLKSQL 481
             L LSL +  T   K   K  +++
Sbjct: 441 QVLCLSLVTIFTNWDKEAKKATNRV 465

BLAST of Spg022623.1 vs. ExPASy Swiss-Prot
Match: F4IHU9 (Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.4e-144
Identity = 258/417 (61.87%), Postives = 326/417 (78.18%), Query Frame = 0

Query: 31  DKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTS 90
           +K  R + + EEV+KQL L+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF S
Sbjct: 16  EKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSLPLSAASIATSFAS 75

Query: 91  VTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQ 150
           VTGFT L+G ASA+DT CGQSYGAK Y MLGI MQRAM VL LLS+PL+++WANT   L 
Sbjct: 76  VTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLV 135

Query: 151 LVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLC 210
             GQD  I+  +G YA+ +IP +FA+GL+QCLNRFLQ QN V P+++ SG+  SLH+++C
Sbjct: 136 FFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIIC 195

Query: 211 WVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKL 270
           WV+V K+GL  RGAA+AN+ISYW+N ++L  YVKFS SCS +WTGFS +A  +I  F+KL
Sbjct: 196 WVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKL 255

Query: 271 AIPSTVMVC-LELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTR 330
            IPS  MVC LE+WSFE++VL SGLLPNP LETS          TVWMIP GLS AASTR
Sbjct: 256 VIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVWMIPFGLSGAASTR 315

Query: 331 VSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVV 390
           VSNELG+G P  AKLA  +V++ +++E +LV TVL+LIR +WG+AYS++ EVV +VA ++
Sbjct: 316 VSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASML 375

Query: 391 PIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGK 447
           PI+++   LD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +LL F  HVGG+
Sbjct: 376 PILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLLLGFHFHVGGR 425

BLAST of Spg022623.1 vs. ExPASy Swiss-Prot
Match: Q9C994 (Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.7e-112
Identity = 223/440 (50.68%), Postives = 299/440 (67.95%), Query Frame = 0

Query: 8   SLNSSLLHISE-DEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMI 67
           S    LL +S+ +EVN KD        F R     E KK  ++AGP+I+V+   Y LQ+I
Sbjct: 3   SAEKGLLVVSDREEVNKKD-------GFLR-----ETKKLSYIAGPMIAVNSSMYVLQVI 62

Query: 68  SIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQR 127
           SIM VGHLGEL LS  ++AVSF SVTGF+V+ G+ASAL+T CGQ+ GAKQY  LG+H   
Sbjct: 63  SIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYT 122

Query: 128 AMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFL 187
            +  L L+ IPL+++W   G+IL L+GQDA ++ EAGK+A  LIP LF +  +Q L RF 
Sbjct: 123 GIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFF 182

Query: 188 QTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFS 247
           Q Q+++ P+++SS  +  +HI+LCW +VFK GL + GAA+A  +SYW+N  +L LY+ FS
Sbjct: 183 QAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFS 242

Query: 248 SSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLS 307
           SSCSKS    S   F  +  F +  IPS  M+CLE WSFE +VLLSG+LPNPKLE SVLS
Sbjct: 243 SSCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLS 302

Query: 308 ISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVL 367
           + L+T  +++ IP  L AAASTRV+NELGAG P  A++A    M I  +E ++V  ++  
Sbjct: 303 VCLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFG 362

Query: 368 IRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSY 427
            RNV+GY +S+E EVV YV  + P++S+S   D L   LSG+ARG G Q IGAYVNL +Y
Sbjct: 363 ARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAY 422

Query: 428 YLVGIPSAVLLAFVLHVGGK 447
           YL GIP+A+LLAF   + G+
Sbjct: 423 YLFGIPTAILLAFGFKMRGR 430

BLAST of Spg022623.1 vs. ExPASy Swiss-Prot
Match: Q8L731 (Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.5e-108
Identity = 204/444 (45.95%), Postives = 311/444 (70.05%), Query Frame = 0

Query: 42  EVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMA 101
           E+K+ ++ A P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A SF +VTGF+ ++G++
Sbjct: 34  ELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASSFCNVTGFSFIIGLS 93

Query: 102 SALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAE 161
            ALDT  GQ+YGAK Y  LG+    AMF L L+ +PL++IW N  ++L ++GQD  I+ E
Sbjct: 94  CALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEKLLLILGQDPSIAHE 153

Query: 162 AGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLET 221
           AGKYA  LIP LFA+ ++Q L R+ Q Q+++ P++++S +   +H+ LCW +V+ +GL  
Sbjct: 154 AGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHVPLCWFLVYNSGLGN 213

Query: 222 RGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLE 281
            G ALA S+S W+ A+ L  ++ +SS+CS++    S + F  I  F K A+PS  M+CLE
Sbjct: 214 LGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEFFKYALPSAAMICLE 273

Query: 282 LWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAA 341
            WS+E+++LLSGLLPNP+LETSVLS+ L T  T++ IP+ ++AAASTR+SNELGAG   A
Sbjct: 274 WWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAASTRISNELGAGNSRA 333

Query: 342 AKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGL 401
           A +     M++AVI+ L+V+  L++ RN++G+ +S+++E + YVA + P++S+S  LD L
Sbjct: 334 AHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAKMAPLVSISLMLDAL 393

Query: 402 QCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLS---KHFL 461
           Q VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +H+ G    + + +      L
Sbjct: 394 QGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTL 453

Query: 462 LLSLPSAPTGTKKYNSKLKSQLSL 483
           LL+L +  T  +    K +++++L
Sbjct: 454 LLALVTGCTNWESQADKARNRMAL 477

BLAST of Spg022623.1 vs. ExPASy TrEMBL
Match: A0A6J1ELR8 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111435773 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.5e-228
Identity = 426/517 (82.40%), Postives = 459/517 (88.78%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S LNS L+HISEDEVN KD R+ DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALIL+
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILM 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHN  YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNFSYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAV EGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVFEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD+VPII+VS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+GGK   + ++S     L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIIS----ALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNVI+
Sbjct: 481 AIITIRTNWDE------EAKIAAERVYESVIPTNVIA 494

BLAST of Spg022623.1 vs. ExPASy TrEMBL
Match: A0A6J1KWH9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 8.2e-227
Identity = 425/517 (82.21%), Postives = 457/517 (88.39%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S L S L+HISEDEVN KD  + DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA LVMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNEQEVVKYVAD+VPII+VS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQL 480
           VNLGSYYLVGIPSAVLLAFVLH+ GK   + ++      L++ +               L
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIDGKGLWLGII----CALAVQTL-------------SL 480

Query: 481 SLIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           ++I ++ +  +      +AKIA ERVY + IPTNVI+
Sbjct: 481 AIITIRTNWDE------EAKIAAERVYESVIPTNVIA 494

BLAST of Spg022623.1 vs. ExPASy TrEMBL
Match: A0A6J1L194 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 6.1e-222
Identity = 425/548 (77.55%), Postives = 457/548 (83.39%), Query Frame = 0

Query: 1   MERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           MERDQ S L S L+HISEDEVN KD  + DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAA LHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSI-------------------------------SLNTDLTVWMIPMGLSAAASTR 360
           ETSVLSI                               SLNTDLTVWMIPMGLSAAASTR
Sbjct: 301 ETSVLSIRYKQRHQFFRSNVIFFCYIQPPLACPTCFFTSLNTDLTVWMIPMGLSAAASTR 360

Query: 361 VSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVV 420
           VSNELGAGRPA AKLAA LVMTIAVIEGLL+ TVL+LIRNVWGYAYSNEQEVVKYVAD+V
Sbjct: 361 VSNELGAGRPAGAKLAACLVMTIAVIEGLLLGTVLILIRNVWGYAYSNEQEVVKYVADIV 420

Query: 421 PIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKASS 480
           PII+VS+F DGLQCVLSGI RGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLH+ GK   
Sbjct: 421 PIIAVSTFFDGLQCVLSGIVRGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHIDGKGLW 480

Query: 481 VHLLSKHFLLLSLPSAPTGTKKYNSKLKSQLSLIPLKFDSPDALIHHIQAKIATERVYNA 518
           + ++      L++ +               L++I ++ +  +      +AKIA ERVY +
Sbjct: 481 LGII----CALAVQTL-------------SLAIITIRTNWDE------EAKIAAERVYES 525

BLAST of Spg022623.1 vs. ExPASy TrEMBL
Match: A0A0A0LV09 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_1G024880 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.9e-221
Identity = 420/516 (81.40%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 2   ERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQY 61
           E  QNSS NS L+H SEDEVNSKDER  D +N RRKLIAEEVKKQLWLAGPLI VSLLQY
Sbjct: 4   EEYQNSSFNSPLIHKSEDEVNSKDERQIDYENIRRKLIAEEVKKQLWLAGPLILVSLLQY 63

Query: 62  CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG 121
           CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG
Sbjct: 64  CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG 123

Query: 122 IHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQC 181
           IH+QRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQC
Sbjct: 124 IHLQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQC 183

Query: 182 LNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILIL 241
           L RFLQTQNVVFPMM+SSGI+A  HI LCW+MVFK GL TRGAA+ANSISYWINALILIL
Sbjct: 184 LYRFLQTQNVVFPMMMSSGISALSHITLCWLMVFKVGLGTRGAAVANSISYWINALILIL 243

Query: 242 YVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE 301
           YVKFSSS SK+WTGFS  AFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE
Sbjct: 244 YVKFSSSSSKTWTGFSRLAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE 303

Query: 302 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVA 361
           TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLA  +VMTIAVIEGLL+A
Sbjct: 304 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAGYVVMTIAVIEGLLLA 363

Query: 362 TVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYV 421
           TVL+L+RNVWGYAYSNEQEVV+YVA+VVP+I+VSSFLDGLQCVLSGIARGCGWQKIGAYV
Sbjct: 364 TVLILVRNVWGYAYSNEQEVVEYVANVVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYV 423

Query: 422 NLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQLS 481
           NLGSYYLVGIPSAVLLAFV HVGGK   + ++      L++ +               L+
Sbjct: 424 NLGSYYLVGIPSAVLLAFVFHVGGKGLWLGII----CALAVQTL-------------SLA 483

Query: 482 LIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           +I ++ +         +AK ATERVY+A IP N IS
Sbjct: 484 IITIRTNWDQ------EAKKATERVYDAVIPVNAIS 496

BLAST of Spg022623.1 vs. ExPASy TrEMBL
Match: A0A1S3BX38 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103494162 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.3e-219
Identity = 417/516 (80.81%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 2   ERDQNSSLNSSLLHISEDEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQY 61
           E  +NSSLNS L+H SED VNSKDE   + +N RRKLIAEEVKKQLWLAGPLISVSLLQY
Sbjct: 4   EEYRNSSLNSPLIHKSEDGVNSKDESQINYENIRRKLIAEEVKKQLWLAGPLISVSLLQY 63

Query: 62  CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG 121
           CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG
Sbjct: 64  CLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLG 123

Query: 122 IHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQC 181
           IH+QRAMF+L L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQC
Sbjct: 124 IHVQRAMFILSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQC 183

Query: 182 LNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILIL 241
           LNRFL TQNVVFPMM+SSGI+A  HI LCW+MVFK GL TRGAA+ANSISYWINALILIL
Sbjct: 184 LNRFLTTQNVVFPMMMSSGISALSHIPLCWLMVFKIGLGTRGAAVANSISYWINALILIL 243

Query: 242 YVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE 301
           YVKFSSS SK+WTGFS  AFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE
Sbjct: 244 YVKFSSSSSKTWTGFSGLAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLE 303

Query: 302 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVA 361
           TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLA  +VMTIAVIEGLL+A
Sbjct: 304 TSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAGCVVMTIAVIEGLLLA 363

Query: 362 TVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYV 421
           T+L+LIRNVWGYAYSNEQEVV+YVA+VVP+I+VSSFLDGLQCVLSGIARGCGWQKIGAYV
Sbjct: 364 TILILIRNVWGYAYSNEQEVVEYVANVVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYV 423

Query: 422 NLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLSKHFLLLSLPSAPTGTKKYNSKLKSQLS 481
           NLGSYYLVGIPSAVLLAFVLHVGGK   + ++      L++ +               L+
Sbjct: 424 NLGSYYLVGIPSAVLLAFVLHVGGKGLWLGII----CALAVQTL-------------SLA 483

Query: 482 LIPLKFDSPDALIHHIQAKIATERVYNAEIPTNVIS 518
           +I ++ +         +AK ATERVY+  IP N IS
Sbjct: 484 IITIRTNWDQ------EAKKATERVYDTVIPVNAIS 496

BLAST of Spg022623.1 vs. TAIR 10
Match: AT5G52450.1 (MATE efflux family protein )

HSP 1 Score: 560.1 bits (1442), Expect = 1.9e-159
Identity = 278/408 (68.14%), Postives = 339/408 (83.09%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF SVTGF+ L+
Sbjct: 23  VKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSFLM 82

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+  I
Sbjct: 83  GTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQNKSI 142

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAG 218
           +  AG YAK +IP +FA+GL+QC NRFLQ QN VFP++  SG+  SLH+LLCWV+VFK+G
Sbjct: 143 ATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVFKSG 202

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  +GAALANSISYW+N ++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PS +MV
Sbjct: 203 LGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSALMV 262

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLE+WSFE++VLLSGLLPNP LETSVLSI LNT  T+WMIP GLS AASTR+SNELGAG 
Sbjct: 263 CLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELGAGN 322

Query: 339 PAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFL 398
           P  AKLA  +V+ IAV E +++ +VL+LIRN+WG AYS+E EVV YVA ++PI+++ +FL
Sbjct: 323 PKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALGNFL 382

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGK 447
           D LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  HVGG+
Sbjct: 383 DSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGR 430

BLAST of Spg022623.1 vs. TAIR 10
Match: AT1G73700.1 (MATE efflux family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 2.9e-152
Identity = 280/445 (62.92%), Postives = 339/445 (76.18%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+ PLI VSLLQY LQ+IS+MFVGHLG LPLS AS+A SF SVTGFT LL
Sbjct: 21  VKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFLL 80

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASAL+T CGQ+YGAK Y  LGI MQRAMFVLL+LS+PL++IWANT +IL LV QD  I
Sbjct: 81  GTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKSI 140

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAG 218
           ++ AG YAK +IP LFA+GL+QC+NRFLQ QN VFP+ V SGI   LH+LLCW+ V K G
Sbjct: 141 ASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKTG 200

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  RGAALA S+SYW N ++L  YVKFS SCS SWTGFS +AF  +  F K+A PS VMV
Sbjct: 201 LGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVMV 260

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLELWSFE++VL SGLLPNP LETSVLSI LNT LT+W I +GL  AAS RVSNELGAG 
Sbjct: 261 CLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAGN 320

Query: 339 PAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFL 398
           P  AKLA  +++ IAV EG++V TVL+ IR + G+A+S++ +++ Y A ++PI++  +FL
Sbjct: 321 PQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNFL 380

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKA---SSVHLLSK 458
           DGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+      V  LS 
Sbjct: 381 DGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRGLWLGIVTALSV 440

Query: 459 HFLLLSLPSAPTGTKKYNSKLKSQL 481
             L LSL +  T   K   K  +++
Sbjct: 441 QVLCLSLVTIFTNWDKEAKKATNRV 465

BLAST of Spg022623.1 vs. TAIR 10
Match: AT2G34360.1 (MATE efflux family protein )

HSP 1 Score: 512.3 bits (1318), Expect = 4.5e-145
Identity = 258/417 (61.87%), Postives = 326/417 (78.18%), Query Frame = 0

Query: 31  DKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTS 90
           +K  R + + EEV+KQL L+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF S
Sbjct: 16  EKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSLPLSAASIATSFAS 75

Query: 91  VTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQ 150
           VTGFT L+G ASA+DT CGQSYGAK Y MLGI MQRAM VL LLS+PL+++WANT   L 
Sbjct: 76  VTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVPLSIVWANTEHFLV 135

Query: 151 LVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLC 210
             GQD  I+  +G YA+ +IP +FA+GL+QCLNRFLQ QN V P+++ SG+  SLH+++C
Sbjct: 136 FFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVICSGVTTSLHVIIC 195

Query: 211 WVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKL 270
           WV+V K+GL  RGAA+AN+ISYW+N ++L  YVKFS SCS +WTGFS +A  +I  F+KL
Sbjct: 196 WVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFSKEARRDIIPFMKL 255

Query: 271 AIPSTVMVC-LELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTR 330
            IPS  MVC LE+WSFE++VL SGLLPNP LETS          TVWMIP GLS AASTR
Sbjct: 256 VIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVWMIPFGLSGAASTR 315

Query: 331 VSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVV 390
           VSNELG+G P  AKLA  +V++ +++E +LV TVL+LIR +WG+AYS++ EVV +VA ++
Sbjct: 316 VSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYSSDPEVVSHVASML 375

Query: 391 PIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGK 447
           PI+++   LD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +LL F  HVGG+
Sbjct: 376 PILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLLLGFHFHVGGR 425

BLAST of Spg022623.1 vs. TAIR 10
Match: AT1G71140.1 (MATE efflux family protein )

HSP 1 Score: 407.9 bits (1047), Expect = 1.2e-113
Identity = 223/440 (50.68%), Postives = 299/440 (67.95%), Query Frame = 0

Query: 8   SLNSSLLHISE-DEVNSKDERIADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMI 67
           S    LL +S+ +EVN KD        F R     E KK  ++AGP+I+V+   Y LQ+I
Sbjct: 3   SAEKGLLVVSDREEVNKKD-------GFLR-----ETKKLSYIAGPMIAVNSSMYVLQVI 62

Query: 68  SIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQR 127
           SIM VGHLGEL LS  ++AVSF SVTGF+V+ G+ASAL+T CGQ+ GAKQY  LG+H   
Sbjct: 63  SIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYT 122

Query: 128 AMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFL 187
            +  L L+ IPL+++W   G+IL L+GQDA ++ EAGK+A  LIP LF +  +Q L RF 
Sbjct: 123 GIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFF 182

Query: 188 QTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFS 247
           Q Q+++ P+++SS  +  +HI+LCW +VFK GL + GAA+A  +SYW+N  +L LY+ FS
Sbjct: 183 QAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFS 242

Query: 248 SSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLS 307
           SSCSKS    S   F  +  F +  IPS  M+CLE WSFE +VLLSG+LPNPKLE SVLS
Sbjct: 243 SSCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLS 302

Query: 308 ISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASLVMTIAVIEGLLVATVLVL 367
           + L+T  +++ IP  L AAASTRV+NELGAG P  A++A    M I  +E ++V  ++  
Sbjct: 303 VCLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFG 362

Query: 368 IRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSY 427
            RNV+GY +S+E EVV YV  + P++S+S   D L   LSG+ARG G Q IGAYVNL +Y
Sbjct: 363 ARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAY 422

Query: 428 YLVGIPSAVLLAFVLHVGGK 447
           YL GIP+A+LLAF   + G+
Sbjct: 423 YLFGIPTAILLAFGFKMRGR 430

BLAST of Spg022623.1 vs. TAIR 10
Match: AT1G15170.1 (MATE efflux family protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-109
Identity = 204/444 (45.95%), Postives = 311/444 (70.05%), Query Frame = 0

Query: 42  EVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMA 101
           E+K+ ++ A P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A SF +VTGF+ ++G++
Sbjct: 34  ELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASSFCNVTGFSFIIGLS 93

Query: 102 SALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAE 161
            ALDT  GQ+YGAK Y  LG+    AMF L L+ +PL++IW N  ++L ++GQD  I+ E
Sbjct: 94  CALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEKLLLILGQDPSIAHE 153

Query: 162 AGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAASLHILLCWVMVFKAGLET 221
           AGKYA  LIP LFA+ ++Q L R+ Q Q+++ P++++S +   +H+ LCW +V+ +GL  
Sbjct: 154 AGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHVPLCWFLVYNSGLGN 213

Query: 222 RGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLE 281
            G ALA S+S W+ A+ L  ++ +SS+CS++    S + F  I  F K A+PS  M+CLE
Sbjct: 214 LGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEFFKYALPSAAMICLE 273

Query: 282 LWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAA 341
            WS+E+++LLSGLLPNP+LETSVLS+ L T  T++ IP+ ++AAASTR+SNELGAG   A
Sbjct: 274 WWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAASTRISNELGAGNSRA 333

Query: 342 AKLAASLVMTIAVIEGLLVATVLVLIRNVWGYAYSNEQEVVKYVADVVPIISVSSFLDGL 401
           A +     M++AVI+ L+V+  L++ RN++G+ +S+++E + YVA + P++S+S  LD L
Sbjct: 334 AHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAKMAPLVSISLMLDAL 393

Query: 402 QCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHVGGKASSVHLLS---KHFL 461
           Q VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +H+ G    + + +      L
Sbjct: 394 QGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTL 453

Query: 462 LLSLPSAPTGTKKYNSKLKSQLSL 483
           LL+L +  T  +    K +++++L
Sbjct: 454 LLALVTGCTNWESQADKARNRMAL 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023531831.12.8e-22982.79protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo][more]
XP_022929082.13.1e-22882.40protein DETOXIFICATION 16-like isoform X1 [Cucurbita moschata][more]
KAG6587669.15.2e-22882.40Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023005095.11.7e-22682.21protein DETOXIFICATION 16-like isoform X2 [Cucurbita maxima][more]
XP_038880134.19.6e-22280.81protein DETOXIFICATION 16-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FHB62.7e-15868.14Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1[more]
Q9C9U14.1e-15162.92Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1[more]
F4IHU96.4e-14461.87Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1[more]
Q9C9941.7e-11250.68Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1[more]
Q8L7312.5e-10845.95Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ELR81.5e-22882.40Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111435773 PE=3 SV=1[more]
A0A6J1KWH98.2e-22782.21Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1[more]
A0A6J1L1946.1e-22277.55Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1[more]
A0A0A0LV093.9e-22181.40Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_1G024880 PE=3 SV=1[more]
A0A1S3BX381.3e-21980.81Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103494162 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52450.11.9e-15968.14MATE efflux family protein [more]
AT1G73700.12.9e-15262.92MATE efflux family protein [more]
AT2G34360.14.5e-14561.87MATE efflux family protein [more]
AT1G71140.11.2e-11350.68MATE efflux family protein [more]
AT1G15170.11.8e-10945.95MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 273..435
e-value: 7.2E-29
score: 100.5
coord: 52..212
e-value: 1.0E-37
score: 129.3
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 52..445
e-value: 8.5E-91
score: 302.7
NoneNo IPR availablePANTHERPTHR11206:SF196PROTEIN DETOXIFICATIONcoord: 22..451
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 22..451
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 42..446
e-value: 1.10154E-178
score: 507.496

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg022623Spg022623gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3391246..3391461CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3391585..3391703CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3392562..3392800CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3392982..3393038CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3393134..3393220CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3393305..3393849CDS
Spg022623.1-cdsSpg022623.1-cds-scaffold2:3394105..3394395CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg022623.1-five_prime_utrSpg022623.1-five_prime_utr-scaffold2:3394396..3394463five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg022623.1Spg022623.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity