Spg022451.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg022451.1 vs. NCBI nr
Match: XP_038880089.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 824.7 bits (2129), Expect = 4.1e-235
Identity = 416/494 (84.21%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDN-------SFSLNQSPKKRRRPDDNGDDHAQFDG- 60
           MEISSFPFLNQE+LLPIFNL SDMDN       +FS+NQSPKKRRR D+NGDDH+QF+  
Sbjct: 1   MEISSFPFLNQEELLPIFNLFSDMDNNHNNTNATFSVNQSPKKRRRSDENGDDHSQFNNI 60

Query: 61  ----ND--LLKLPLWFDTHDDKPEHSWIMDSEADH-KHEFHLSDESFPQFSKKPRRETPA 120
               ND  L KLP WF++     + +WIMDSE    ++EFHLSD++  QFSKKPRR T  
Sbjct: 61  SFTENDEALQKLPCWFES-----QENWIMDSEEPKPRNEFHLSDQNPTQFSKKPRRTT-- 120

Query: 121 SANSSPAKGGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM 180
           + N SPAK    GG Q    QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM
Sbjct: 121 AENGSPAKNPTGGGAQ----QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDM 180

Query: 181 ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV 240
           ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV
Sbjct: 181 ICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEV 240

Query: 241 CSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN 300
           CSAIRKVLMPKFLQWPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN
Sbjct: 241 CSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFN 300

Query: 301 KRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDV 360
           KRHTERNQKTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKS LFERANMGLLNDV
Sbjct: 301 KRHTERNQKTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSVLFERANMGLLNDV 360

Query: 361 FIVGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTE 420
           FIVGNSGYPL DWLLVPY++QNLTWTQH FNEKV EIQ AAK AFGRLKGRWSCLQKRTE
Sbjct: 361 FIVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKTAFGRLKGRWSCLQKRTE 420

Query: 421 VKLQELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIA 480
           VKLQELPVVLGACCVLHNICE+RKE FDP+L FEL+DDEM+PE+NGLRSVSAIQARDHIA
Sbjct: 421 VKLQELPVVLGACCVLHNICEIRKEKFDPDLKFELYDDEMVPENNGLRSVSAIQARDHIA 480

BLAST of Spg022451.1 vs. NCBI nr
Match: XP_022933888.1 (protein ALP1-like [Cucurbita moschata] >KAG7021950.1 Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 822.8 bits (2124), Expect = 1.6e-234
Identity = 414/486 (85.19%), Postives = 438/486 (90.12%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDH------AQFDGND 60
           MEISSFPFLNQ+DLLPIFNL S+MD++FS+NQSPKKRRR +D+ DDH        FD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAK 120
           LLKLP WFD  DDK +H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDST 180
           GG   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDST
Sbjct: 121 GG--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300
           MPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGY 360
           KTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGY
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGY 360

Query: 361 PLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPV 420
           PLTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGL 473

BLAST of Spg022451.1 vs. NCBI nr
Match: KAG6588056.1 (Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 821.6 bits (2121), Expect = 3.5e-234
Identity = 413/486 (84.98%), Postives = 438/486 (90.12%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDH------AQFDGND 60
           MEISSFPFLNQ+DL+PIFNL S+MD++FS+NQSPKKRRR +D+ DDH        FD ++
Sbjct: 1   MEISSFPFLNQDDLVPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAK 120
           LLKLP WFD  DDK +H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDST 180
           GG   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDST
Sbjct: 121 GG--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300
           MPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGY 360
           KTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGY
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGY 360

Query: 361 PLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPV 420
           PLTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGL 473

BLAST of Spg022451.1 vs. NCBI nr
Match: XP_023529395.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.3e-233
Identity = 413/486 (84.98%), Postives = 435/486 (89.51%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDD------HAQFDGND 60
           MEISSFPFLNQ+DLLPIFNL S+MD++FS+N SPKKRRR D++ DD         FD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNHSPKKRRRQDNDDDDDQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAK 120
           LLKLP WFD  DDK +H WIM    + K EF +SDE+  QF  KKPRR TP + +SSP K
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPVK 120

Query: 121 GGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDST 180
           GG   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMICKELDST
Sbjct: 121 GG--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICKELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300
           MPKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGY 360
           KTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGY
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGY 360

Query: 361 PLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPV 420
           PLTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGL 473

BLAST of Spg022451.1 vs. NCBI nr
Match: XP_022967341.1 (protein ALP1-like [Cucurbita maxima])

HSP 1 Score: 817.8 bits (2111), Expect = 5.0e-233
Identity = 412/485 (84.95%), Postives = 436/485 (89.90%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDHAQ-----FDGNDL 60
           MEISSFPFLNQ+DLLPIFNL S+MD++FS+N SPKKRRR DD+ DD  Q     FD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAKG 120
           LKLP WFD  DDK +H WIM    + K EF + DE+  QF +KKPRR TP + +SSPAKG
Sbjct: 61  LKLPFWFD--DDKQQH-WIM----EQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAKG 120

Query: 121 GGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTV 180
           G   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTV
Sbjct: 121 G--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTV 180

Query: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240
           MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM
Sbjct: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240

Query: 241 PKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300
           PKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK
Sbjct: 241 PKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300

Query: 301 TSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYP 360
           TSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYP
Sbjct: 301 TSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYP 360

Query: 361 LTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVV 420
           LTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVV
Sbjct: 361 LTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVV 420

Query: 421 LGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLA 480
           LGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAI ARDHI+HNLLHHGLA
Sbjct: 421 LGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLA 472

BLAST of Spg022451.1 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.3e-38
Identity = 113/362 (31.22%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 134 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 193
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 194 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 253
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 254 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 313
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 314 QGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMG-LLND------------VFIV 373
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 374 GNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEV-K 433
           G+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 434 LQELPVVLGACCVLHN-ICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAH 469
              LP ++  CC+LHN I +M  ++ D +   +  D      S  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Spg022451.1 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.9e-34
Identity = 94/314 (29.94%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 137 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 196
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 197 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 256
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 257 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 316
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 317 VDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLND-------------VFIVGNS 376
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 377 GYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQK-RTEVKLQE 425
            YPL  WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Spg022451.1 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-17
Identity = 69/261 (26.44%), Postives = 121/261 (46.36%), Query Frame = 0

Query: 169 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 228
           EL    + + T    AI    +V   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGANLSRPTQRSRAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 229 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 288
           + +    +F+++P D++ +  +K EF  ++G+P V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIRFPADEASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLSYVNR- 170

Query: 289 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 348
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMHKDSWL 230

Query: 349 VGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVK 408
           +G+S + L  WL+ P  +   T  ++ +N   S   +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 409 LQELPV----VLGACCVLHNI 425
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Spg022451.1 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 7.2e-17
Identity = 68/261 (26.05%), Postives = 120/261 (45.98%), Query Frame = 0

Query: 169 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 228
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 229 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 288
           + +    +F+ +P D++ +  +K EF  ++GIP V G +   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYVNR- 170

Query: 289 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 348
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  + ++
Sbjct: 171 ------KGLHSLNCLMVCDIRGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMHKESWL 230

Query: 349 VGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVK 408
           +G+S + L  WL+ P  +   T  ++ +N   S   +  +  F  L  R+ CL   ++  
Sbjct: 231 LGDSSFFLRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLD-GSKGA 290

Query: 409 LQELPV----VLGACCVLHNI 425
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Spg022451.1 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 7.2e-17
Identity = 68/261 (26.05%), Postives = 120/261 (45.98%), Query Frame = 0

Query: 169 ELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSA 228
           EL    + + T    AI    ++   +    +G     +    G+  ++  + V  V  A
Sbjct: 51  ELLGASLSRPTQRSRAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEA 110

Query: 229 IRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKR 288
           + +    +F+ +P D++ +  +K EF  ++G+P V G++   H+ I AP     +Y N+ 
Sbjct: 111 LVE-RASQFIHFPADEAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYVNR- 170

Query: 289 HTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFI 348
                 K  +S+    V D  G    V   WPGS+ D  VL++S+L  +   G+  D ++
Sbjct: 171 ------KGLHSLNCLVVCDIRGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKDSWL 230

Query: 349 VGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVK 408
           +G+S + L  WLL P  +   T  ++ +N   S   +  +     L  R+ CL   ++  
Sbjct: 231 LGDSSFFLHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLD-GSKGA 290

Query: 409 LQELPV----VLGACCVLHNI 425
           LQ  P     ++ ACCVLHNI
Sbjct: 291 LQYSPEKSSHIILACCVLHNI 301

BLAST of Spg022451.1 vs. ExPASy TrEMBL
Match: A0A6J1F642 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111441167 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.5e-235
Identity = 414/486 (85.19%), Postives = 438/486 (90.12%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDH------AQFDGND 60
           MEISSFPFLNQ+DLLPIFNL S+MD++FS+NQSPKKRRR +D+ DDH        FD ++
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNQSPKKRRRQNDDDDDHQTQFNKTSFDDDE 60

Query: 61  LLKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAK 120
           LLKLP WFD  DDK +H WIM    + K EF +SDE+  QF  KKPRR TP + +SSPAK
Sbjct: 61  LLKLPFWFD--DDKQQH-WIM----EQKPEFQVSDENLTQFVPKKPRRATPENTHSSPAK 120

Query: 121 GGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDST 180
           GG   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDST
Sbjct: 121 GG--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDST 180

Query: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240
           VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL
Sbjct: 181 VMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVL 240

Query: 241 MPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300
           MPKFLQWP++SKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ
Sbjct: 241 MPKFLQWPEESKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQ 300

Query: 301 KTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGY 360
           KTSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGY
Sbjct: 301 KTSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGY 360

Query: 361 PLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPV 420
           PLTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPV
Sbjct: 361 PLTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPV 420

Query: 421 VLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGL 480
           VLGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAIQARDHIAHNLLHHGL
Sbjct: 421 VLGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAIQARDHIAHNLLHHGL 473

BLAST of Spg022451.1 vs. ExPASy TrEMBL
Match: A0A6J1HRQ5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111466889 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.4e-233
Identity = 412/485 (84.95%), Postives = 436/485 (89.90%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDHAQ-----FDGNDL 60
           MEISSFPFLNQ+DLLPIFNL S+MD++FS+N SPKKRRR DD+ DD  Q     FD ++L
Sbjct: 1   MEISSFPFLNQDDLLPIFNLFSEMDDNFSVNYSPKKRRRQDDDDDDQTQFNKTSFDDDEL 60

Query: 61  LKLPLWFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQF-SKKPRRETPASANSSPAKG 120
           LKLP WFD  DDK +H WIM    + K EF + DE+  QF +KKPRR TP + +SSPAKG
Sbjct: 61  LKLPFWFD--DDKQQH-WIM----EQKPEFQVFDENLTQFVAKKPRRATPENTHSSPAKG 120

Query: 121 GGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTV 180
           G   G Q     RRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTV
Sbjct: 121 G--AGTQ----HRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICQELDSTV 180

Query: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240
           MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM
Sbjct: 181 MKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLM 240

Query: 241 PKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300
           PKFLQWP+DSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK
Sbjct: 241 PKFLQWPEDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQK 300

Query: 301 TSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYP 360
           TSYSITVQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLL DV IVGNSGYP
Sbjct: 301 TSYSITVQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLKDVSIVGNSGYP 360

Query: 361 LTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVV 420
           LTDWLLVPYS QNLTWTQHAFNEKVSEIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVV
Sbjct: 361 LTDWLLVPYSAQNLTWTQHAFNEKVSEIQGAAKAAFGRLKGRWTCLQKRTEVKLQELPVV 420

Query: 421 LGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLA 480
           LGACCVLHNICEMRKE FDPEL FE FDDEM+PE+NG+RS SAI ARDHI+HNLLHHGLA
Sbjct: 421 LGACCVLHNICEMRKERFDPELKFEFFDDEMVPENNGVRSASAILARDHISHNLLHHGLA 472

BLAST of Spg022451.1 vs. ExPASy TrEMBL
Match: A0A1S3BPN5 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103492343 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.8e-229
Identity = 405/496 (81.65%), Postives = 432/496 (87.10%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNS----FSLNQSPKKRRRPDDNGDDHAQF---DGN 60
           MEISSFPFLNQE+ LPIFNL SDMDN+    F++N +PKKRRR D N DD   F   D N
Sbjct: 1   MEISSFPFLNQEEFLPIFNLFSDMDNNPTTPFNVNPTPKKRRRSDPNSDDFNNFSFTDEN 60

Query: 61  D------LLKLPLWFDTHDDKPEHSWIMDSEADH-KHEFHLSDESFPQFSKKPRR---ET 120
           D      LLKLP WFD   + P+ SW+MDS+     ++FHLSD    Q  KKPRR   E 
Sbjct: 61  DEPTDDPLLKLPCWFDPQPESPQ-SWLMDSQKPKPTNDFHLSD----QIPKKPRRASPEN 120

Query: 121 PASANSSPAKGGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTF 180
           P+   ++PA GGGT        QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTF
Sbjct: 121 PSPVKNNPAGGGGT-------QQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTF 180

Query: 181 DMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVL 240
           DMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVL
Sbjct: 181 DMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVL 240

Query: 241 EVCSAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAY 300
           EVCSAIRKVLMPKFLQWPD+SKL KIKQEFESISGIPKVGGSIYTTHIPIIAP+NNVAAY
Sbjct: 241 EVCSAIRKVLMPKFLQWPDESKLTKIKQEFESISGIPKVGGSIYTTHIPIIAPRNNVAAY 300

Query: 301 FNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLN 360
           FNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKS L+ERA+MGLLN
Sbjct: 301 FNKRHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSLLYERASMGLLN 360

Query: 361 DVFIVGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKR 420
           DVF+VGNSGYPL DWLLVPY++QNLTWTQH FNEKV EIQ AAKAAFGRLKGRW+CLQKR
Sbjct: 361 DVFVVGNSGYPLMDWLLVPYTVQNLTWTQHGFNEKVGEIQAAAKAAFGRLKGRWTCLQKR 420

Query: 421 TEVKLQELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDH 480
           TEVKLQELPVVLGACCVLHNICEMRKE FDPEL FE++DDEM+PE+NGLRSVSAIQARDH
Sbjct: 421 TEVKLQELPVVLGACCVLHNICEMRKEKFDPELKFEVYDDEMLPENNGLRSVSAIQARDH 480

BLAST of Spg022451.1 vs. ExPASy TrEMBL
Match: A0A6J1HAH8 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111461585 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.1e-228
Identity = 397/479 (82.88%), Postives = 423/479 (88.31%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDHAQFDGNDLLKLPL 60
           MEI+SFPFLNQ++ LPI N  S+MDN FS+NQSPKKRRRP DNGDD +   GNDLL  P 
Sbjct: 1   MEITSFPFLNQDEFLPISNFFSEMDN-FSVNQSPKKRRRPTDNGDDGSGNGGNDLLNHPF 60

Query: 61  WFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQFSKKPRRETPASANSSPAKGGGTGGQ 120
           W +  D++ EH W+MDS            + F   SKKPRR TP + NSSPAKGG     
Sbjct: 61  WVENRDEQREH-WVMDS------------DEFQVLSKKPRRGTPQNDNSSPAKGGAGA-- 120

Query: 121 QQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTM 180
                QRRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTM
Sbjct: 121 ----HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTM 180

Query: 181 LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW 240
           LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW
Sbjct: 181 LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW 240

Query: 241 PDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSIT 300
           P++SKL KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSIT
Sbjct: 241 PEESKLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSIT 300

Query: 301 VQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWLL 360
           VQGVVDP+GVFTDVCIGWPGSMPDD VLEKSALFERANMGLLNDVFIVGNSGYPL DWLL
Sbjct: 301 VQGVVDPAGVFTDVCIGWPGSMPDDHVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLL 360

Query: 361 VPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCV 420
           VPY++QNLTWTQHAFNEKV EIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACCV
Sbjct: 361 VPYTLQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCV 420

Query: 421 LHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 480
           LHNICEMRKE FD EL FE+FDDEM+PESNG+RSVSAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 LHNICEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Spg022451.1 vs. ExPASy TrEMBL
Match: A0A6J1JHN7 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111485185 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.9e-228
Identity = 396/479 (82.67%), Postives = 423/479 (88.31%), Query Frame = 0

Query: 1   MEISSFPFLNQEDLLPIFNLLSDMDNSFSLNQSPKKRRRPDDNGDDHAQFDGNDLLKLPL 60
           MEI+S PFLNQ++ LPI N  S++DN FS+NQS KKRRRP DNGDD +   GNDLL LP 
Sbjct: 1   MEITSVPFLNQDEFLPISNFFSEIDN-FSVNQSLKKRRRPTDNGDDGSGNGGNDLLNLPF 60

Query: 61  WFDTHDDKPEHSWIMDSEADHKHEFHLSDESFPQFSKKPRRETPASANSSPAKGGGTGGQ 120
           W +  D++ EH W+MDS            + F   SKKPRR TP + NSSPAKGG     
Sbjct: 61  WVENRDEQQEH-WVMDS------------DEFQVLSKKPRRGTPQNDNSSPAKGGAGA-- 120

Query: 121 QQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKKDTM 180
                QRRLWVK+RSKDWWDQCNHPDFPDEEFRRAFRMSK+TFDMIC+ELDSTVMKKDTM
Sbjct: 121 ----HQRRLWVKNRSKDWWDQCNHPDFPDEEFRRAFRMSKATFDMICRELDSTVMKKDTM 180

Query: 181 LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW 240
           LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW
Sbjct: 181 LRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQW 240

Query: 241 PDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSIT 300
           P++SKL KIKQEFESISGIPKVGG+IYTTHIPIIAPKN+VAAYFNKRHTERNQKTSYSIT
Sbjct: 241 PEESKLTKIKQEFESISGIPKVGGAIYTTHIPIIAPKNSVAAYFNKRHTERNQKTSYSIT 300

Query: 301 VQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLTDWLL 360
           VQGVVDP+GVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPL DWLL
Sbjct: 301 VQGVVDPAGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLNDVFIVGNSGYPLMDWLL 360

Query: 361 VPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCV 420
           VPY++QNLTWTQHAFNEKV EIQ AAKAAFGRLKGRW+CLQKRTEVKLQELPVVLGACCV
Sbjct: 361 VPYTLQNLTWTQHAFNEKVGEIQAAAKAAFGRLKGRWTCLQKRTEVKLQELPVVLGACCV 420

Query: 421 LHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAHNLLHHGLAGTGFL 480
           LHNICEMRKE FD EL FE+FDDEM+PESNG+RSVSAIQ RDHIAHNLLHHGLAGTGFL
Sbjct: 421 LHNICEMRKERFDSELKFEVFDDEMVPESNGVRSVSAIQGRDHIAHNLLHHGLAGTGFL 459

BLAST of Spg022451.1 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 566.2 bits (1458), Expect = 2.5e-161
Identity = 270/374 (72.19%), Postives = 318/374 (85.03%), Query Frame = 0

Query: 107 ANSSPAKGGGTGGQQQQQPQRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMI 166
           A SS   G G+        QRRLWVKDRS+ WW++C+  D+P+E+F++AFRMSKSTF++I
Sbjct: 130 AESSEITGSGSVRGTGSGQQRRLWVKDRSRAWWEECSRLDYPEEDFKKAFRMSKSTFELI 189

Query: 167 CKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVC 226
           C EL+S V K+DT LR AIPVRQRVAVCIWRLATGEPLRLVSK+FGLGISTCHKLVLEVC
Sbjct: 190 CDELNSAVAKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVC 249

Query: 227 SAIRKVLMPKFLQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNK 286
            AI+ VLMPK+LQWPDD  L  I++ FES+SGIP V GS+YTTHIPIIAPK +VA+YFNK
Sbjct: 250 KAIKDVLMPKYLQWPDDESLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNK 309

Query: 287 RHTERNQKTSYSITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANM-GLLNDV 346
           RHTERNQKTSYSIT+Q VV+P GVFTD+CIGWPGSMPDD+VLEKS L++RAN  GLL  +
Sbjct: 310 RHTERNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM 369

Query: 347 FIVGNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTE 406
           ++ G  G+PL DW+LVPY+ QNLTWTQHAFNEK+SE+Q  AK AFGRLKGRW+CLQKRTE
Sbjct: 370 WVAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQKRTE 429

Query: 407 VKLQELPVVLGACCVLHNICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIA 466
           VKLQ+LP VLGACCVLHNICEMR+E  +PEL  E+ DDE++PE N LRSV+A++ARD I+
Sbjct: 430 VKLQDLPTVLGACCVLHNICEMREEKMEPELMVEVIDDEVLPE-NVLRSVNAMKARDTIS 489

Query: 467 HNLLHHGLAGTGFL 480
           HNLLHHGLAGT FL
Sbjct: 490 HNLLHHGLAGTSFL 502

BLAST of Spg022451.1 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 548.5 bits (1412), Expect = 5.3e-156
Identity = 303/540 (56.11%), Postives = 360/540 (66.67%), Query Frame = 0

Query: 1   MEISSFPF--LNQEDLLPIFNLLSDMD-----------NSFSLNQSPKKRRRPDD----- 60
           MEISSFPF  L  ++      L  DMD           NS   N + KKR R DD     
Sbjct: 1   MEISSFPFPYLQDDECSHFLGLFQDMDSSPSTFGLEGFNSNDNNTNQKKRPRKDDEGGGG 60

Query: 61  -----------NGDDHAQFDGNDLLKLPLWFDTHDDKPEHSWIMD-------SEADHKHE 120
                      NG++ A F   D+L   L  D    + +  W  +        EA+HK +
Sbjct: 61  GGGGTEVLGAVNGNNKAAF--GDILATLLLLDEEAKQQQEQWDFEFIKEKSLLEANHKKK 120

Query: 121 FHLSDESFPQF--------------SKKPRRETPASANSSPAKGGGTGGQQQQQP----- 180
               D  + Q               SK+ R+   A+  S+ A G  T G     P     
Sbjct: 121 VKTMDGYYNQMQDHYSAAGETDGSRSKRARKTAVAAVVSAVASGADTTGLAAPVPTADIA 180

Query: 181 --------QRRLWVKDRSKDWWDQCNHPDFPDEEFRRAFRMSKSTFDMICKELDSTVMKK 240
                    RRLWVK+R+ DWWD+ + PDFP++EFRR FRMSKSTF++IC+ELD+TV KK
Sbjct: 181 SGSGSGPSHRRLWVKERTTDWWDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTKK 240

Query: 241 DTMLRVAIPVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKF 300
           +TMLR AIP  +RV VC+WRLATG PLR VS+RFGLGISTCHKLV+EVC AI  VLMPK+
Sbjct: 241 NTMLRDAIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKY 300

Query: 301 LQWPDDSKLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSY 360
           L WP DS++   K +FES+  IP V GSIYTTHIPIIAPK +VAAYFNKRHTERNQKTSY
Sbjct: 301 LLWPSDSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSY 360

Query: 361 SITVQGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALF-ERANMGLLNDVFIVGNSGYPLT 420
           SITVQGVV+  G+FTDVCIG PGS+ DDQ+LEKS+L  +RA  G+L D +IVGNSG+PLT
Sbjct: 361 SITVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSWIVGNSGFPLT 420

Query: 421 DWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLG 477
           D+LLVPY+ QNLTWTQHAFNE + EIQ  A AAF RLKGRW+CLQKRTEVKLQ+LP VLG
Sbjct: 421 DYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLG 480

BLAST of Spg022451.1 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-39
Identity = 113/362 (31.22%), Postives = 175/362 (48.34%), Query Frame = 0

Query: 134 RSKDWWDQCNHPDF----PDEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVA----I 193
           +S DWWD  +   +      + F   F++S+ TFD IC  + +    K      +    +
Sbjct: 50  QSLDWWDGFSRRIYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPL 109

Query: 194 PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDSK 253
            +  RVAV + RL +GE L ++ + FG+  ST  ++      ++ +  +   L WP  SK
Sbjct: 110 SLNDRVAVALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAI-HHLSWP--SK 169

Query: 254 LAKIKQEFESISGIPKVGGSIYTTHI----PIIAPKNNVAAYFNKRHTERNQKTSYSITV 313
           L +IK +FE ISG+P   G+I  THI    P + P N V           + + ++S+T+
Sbjct: 170 LDEIKSKFEKISGLPNCCGAIDITHIVMNLPAVEPSNKVWL---------DGEKNFSMTL 229

Query: 314 QGVVDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMG-LLND------------VFIV 373
           Q VVDP   F DV  GWPGS+ DD VL+ S  ++    G  LN              +IV
Sbjct: 230 QAVVDPDMRFLDVIAGWPGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIV 289

Query: 374 GNSGYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEV-K 433
           G+SG+PL  WLL PY  +  +  Q  FN++ SE   AA+ A  +LK RW  +     +  
Sbjct: 290 GDSGFPLLPWLLTPYQGKPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPD 349

Query: 434 LQELPVVLGACCVLHN-ICEMRKESFDPELNFELFDDEMMPESNGLRSVSAIQARDHIAH 469
              LP ++  CC+LHN I +M  ++ D +   +  D      S  L   ++   RD ++ 
Sbjct: 350 RNRLPRIIFVCCLLHNIIIDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSD 399

BLAST of Spg022451.1 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 147.9 bits (372), Expect = 2.1e-35
Identity = 94/314 (29.94%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 137 DWWD----QCNHPDFPDEE---FRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAI---- 196
           DWWD    + + P  P +E   F+  FR SK+TF  IC  +   ++ +     + I    
Sbjct: 43  DWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRL 102

Query: 197 -PVRQRVAVCIWRLATGEPLRLVSKRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWPDDS 256
             V ++VA+ + RLA+G+    V   FG+G ST  ++      A+ +      L+WPD  
Sbjct: 103 LSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTVSQVTWRFIEALEE-RAKHHLRWPDSD 162

Query: 257 KLAKIKQEFESISGIPKVGGSIYTTHIPIIAPKNNVAAYFNKRHTERNQKTSYSITVQGV 316
           ++ +IK +FE + G+P   G+I TTHI +  P    +  +       +Q+ +YS+ +QGV
Sbjct: 163 RIEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAVQASDDW------CDQEKNYSMFLQGV 222

Query: 317 VDPSGVFTDVCIGWPGSMPDDQVLEKSALFERANMGLLND-------------VFIVGNS 376
            D    F ++  GWPG M   ++L+ S  F+      + D              ++VG  
Sbjct: 223 FDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGI 282

Query: 377 GYPLTDWLLVPYSMQNLTWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQK-RTEVKLQE 425
            YPL  WL+ P+   + + +  AFNE+  ++++ A  AF +LKG W  L K       ++
Sbjct: 283 SYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRK 342

BLAST of Spg022451.1 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 104.8 bits (260), Expect = 2.0e-22
Identity = 80/309 (25.89%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 149 DEEFRRAFRMSKSTFDMICKELDSTVMKKDTMLRVAIPVRQRVAVCIWRLATGEPLRLVS 208
           D  +R  + +S   F  +  +L   +    T   +++P    VA+ + RLA G   + ++
Sbjct: 114 DARWRSLYGLSYPVFITVVDKLKPFI----TASNLSLPADYAVAMVLSRLAHGCSAKTLA 173

Query: 209 KRFGLGISTCHKLVLEVCSAIRKVLMPKFLQWP-DDSKLAKIKQEFESISGIPKVGGSIY 268
            R+ L      K+   V   +   L P+F++ P    +L +  Q FE ++ +P + G+I 
Sbjct: 174 SRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAID 233

Query: 269 TTHIPIIAPKNNVAAYFNKRHTERNQKTSY-------SITVQGVVDPSGVFTDVCIGWPG 328
           +T + +            +R T+ N +  Y       ++ +Q V D   +F DVC+  PG
Sbjct: 234 STPVKL------------RRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPG 293

Query: 329 SMPDDQVLEKSALFERANMG------LLN------DVFIVGNSGYPLTDWLLVPYSMQNL 388
              D      S L++R   G      ++N        +IVG+  YPL  +L+ P+S    
Sbjct: 294 GEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 353

Query: 389 -TWTQHAFNEKVSEIQTAAKAAFGRLKGRWSCLQKRTEVKLQELPVVLGACCVLHNICEM 437
            T  ++ F+  + + ++    A G LK RW  LQ    V +   P  + ACCVLHN+C++
Sbjct: 354 GTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQS-LNVGVNHAPQTIVACCVLHNLCQI 404