Spg020825.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg020825.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCysteinyl-tRNA synthetase
Locationscaffold10: 28411338 .. 28416485 (-)
Sequence length702
RNA-Seq ExpressionSpg020825.1
SyntenySpg020825.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGACCTTTCTTTCCAGTTCAGTCATTCTTTCTTCGCTTGTACCTATGTCGACCATCATTACAGACATCACGTCAGGATCTGATGTCTCCTTTGCCTGCTCAGAAGATGAAGTAGAATAGTCAAATAAAGGATTTTCCTGCATGACCATCCTGCCTTGGTTTGGCTCTACCATTTGAGTTCATATCATATTTGCAATGATAGAGGCTTGTTCTTCTTCTGATTTCTGGATTTCCTTGGAGCGTCCGCGAGTAACTGGTCCCACGTAGGAGTCGCTTGAAGTCTTGGAAGCAACTTTCTTGGATGCCATCGTTTCTTTAGAACTTTTTTCGTAGATCTTTAATGAGAGAAAGAGATGAAAGGTAGAGAGGTCCAAACTATTGAATTCTGAGAGCTTTCTTCAGAGTCTTCTGTATATGTGTAGTGGAGTACAAACTGTTAACACGAGATTTCAGGAGAGAAATTTGATTCGTGAAACGACTTCAAATATGTATTGATTTCTGTATATGTGTAGTGGAGTACAATCTCTAGTTACAATCCTTTGATGTTTTCTCTCACAAACTCAAGTTAGCTTGATCTACAGAGTCTTCAGGAAGCTTTAGTCTTCAGAGTCCTGAAGGTTTTTTCTTCAGAAGGATTTGAATTCTGAGAGCTTTCGCTTCTTCCTTTCTCCCTGCCCTCCCCAAATGAGAGGTCTTGCCTCCTATTTATAGAGTTTTCCAAAGGCCCTTTGTGGGCTTGGGCTTGGTTGCATTTGGGCCCATTCATAGGCTTGGACTTAAAATGCATTTGGGCCCATTCATGGGCTTGGTCTTATCAAATTTGGACTTCTGACCCAATTACATTATGGAATTCGGGTTTAAATTGAACCCAATTCAATCTATTTCATGAAATTAGCATATTTTAATTTCACGAACTTAGACAAATTATTTGAAGAAAAATTTCTATGCCAACAATCACAAACAAATAAAATAATCATTTACTTGCACGTAAAGATCGTGTCTTGAAGAGATGACCCAATTTGTTCAACGAGGTAGTCTTCTCTCTCTAAGAGATGACTTCATGATACGTGGTGAAGTCGTCTCTGAAAGAGGCAATCTCGTGTTTAAAACAACAATACCTAAAAATAAGTTAAAAAAATATTTTCCAAAAGTAAGTTTTAAAAATAATAATATAAAACTTCTAAAAAAAATAATAATAATATAAAATAATATTCCCCACCAGTCCACCCCTTACTTTCTTCCCCATATCTCCCTCTTTGCTACAATTGTATGTCGATAAAAGTGATAAAAGGGAAAAAAACGTCATTATAATAAATTGAAAAGAAAACTTCTCTCTTATGTTTTTATACATAGAAGACATAGACAAATATATATGATTAAATTATTGTTATTGTTTTAAATTAACTATAAATAATTAAATTCAAACTTTTATAAAAATAAAAATAAAAATACTATTATTTAATTCAAATTTAAAACTTCGTTTTATCCACCCAGTTCGACAATCCAACGAACCCAGTATATCAAAAGAGGGAAAAAAAAAAACAAGAAGAAATAGAGAGAGTAGCGTGTGCGTTGAGTTTATTTTTCTGCTAATTTTGTATGTTAATTAAATAAGACAATTGCGGATTTTTATTTTATATTTTATTTTATTATTTTTTACAACATTGGGGATCAATTTTGTAGGGTGATGGAAATTGGAAAGCATAGGAGGCAGTAGTGGGAGCTTCACTAAGATAGGTTCTTTTGGTCAGCACACAAATGATTATATTATGTGGGTGGAAAATAATGTATGAAACTACTATTGTATCCTTTTATTATCTTACATGGTTCAAAATTCCAAGAAATTTACAGTTGGAGAGTAAGTTGAGTTCTGCCAACTTAAGCATAATTTGATTAGTTAGATATTATATTATAACATAACAATTGCAACAATTAAAAGAAAAAAGACAGTGATTTGTGATCCACCACAAAGGCATTGGGCTTCAATGATTCCAATATCTTTTTGGAAGTTGTGCCTTTAGAACTTCTGGGCCAAAGTTTGAGAGGAAAATTTCAATCTTTGACATATTTGGTTCAAAGTTAGAACTCTTCATTTGTCCACCCATCTTTTTATAAGTTTTGCCAAAGCGAGCATAGCTAAGTGGTAATTGACATGTACTTTCGACCAAGAGGTCAGAGGTTCGAATCTCCCCACCCCCAAATATTGATATACTAAAAAAAAAAAATCTTTTTACAAGTTTTTCTTTTTGGTTGAATCTTTTCAATATTGGAAATTTTAACTTTAACCCCTGTTTAGAATCCTTAAATTTGAGATGAATTTCAAATTAAATTTGCTGATTTCAAATCTTTTTTTTTTTTAATCCATTCCAAATAGCTCGAATTTGGCAGAATTTTAAACTTAAAAAATTTGATTCCAATTTTAACCCTCAACTCTTTTTCTTTTATTCGATAAGCTTCCCATCATATCGACTCTCACTACCTCTTCCCAGACTCAAAGCCATTTGACGCAAGCACACAGTCAAGGTTATAGATTTCTATTTTATTTGCTCTTGTATTTTGCCATTTTTAAAAAACTTATAATGGTAATTATAATCTTTTCAATGTAGCTTTACGATTCCAACCAGATTTTATAGTCAAGCAAACTAAATCAAAGGTAAGTTTTTTTTCCCTTTATGTGTTCATCTTTTTAAACTATTTTTTTCCGTATTATTTTCTTTTTGATCAGTGAACTGATATGGGTACTGTTCAATTTTGTGTTCTGTGTAATCTTTTTCATTCTTTTTTAATTTTTTTTTATCAACTCTGTAATCTGTTTTCTATTTTGATAGTATCTTTTGATAATTCATACTGTTTTTTTTAAATAAATTTGTTTGATTAATCAAAATTTGTAGTTTTATTGCATTTGCGGGAAGTAATTTGTCCTCTAATTTTGAAATATTTTGAAATTCCATTCAAAATCCATATCTTCAAATGTTGGTTTGTTATTCTATTTTGATTTATCAATGGATTCTGTAGTAAGTCAAGTCAGTAGTTAGGCTCAGTTTTTAACCTATTTATATTAGATATGTATTCCAAAAATTAATTTAATATAGAGCATCTCATTTTAATTAATATATAATAATATATATAGGAAGAAAAAAAGGATGAGAGTAGGATTATTGTAATGTATTTTTATTAAAAATGTAACCCCAAAGCCGCTAATAATCTAGGCAACTTTCGAAACAATTTCTTTCGAGGATTAGTTCGATTTTTTTTTCTTCGTTTGCATAAAGATATTTGTGTTTTTTTTTTTAAAATTTTCTTATTTACAATAATGAAGACATTTACAAATTTTAACTTAATAAACAAATTTATTTTTATCAATTGTATGTTTCATGGTTGTATGTGTTTAATACTGTTATTTGACATTAAAATATTTTGCAATTGATTAAAATTATTTCTTGCAAATTTATAGAGCATAGCAGTAAAATGTTATACTTTCTTTTCATTTATCGTGAGCATCCAAATAAGAGATTTTAATTTTAATGATTTCAAATCAATTTGGTTCCAAATGCAAATAGAAATTTGAAATAATTTCCATCTTTTAAAATCCTAGAATTTAAATTCATTTCTTGTCAATTCCATGCACCAAACAAAGCCTAAGATTAGTTTTAAAACTCTTAAGTTATTTGAAACTTAAGTTCAACTTTACAATTAATTGAGTCCGCTTAAATACATTAAATATGACCTATTTGTAATTTATTACTTCAGTAGAACGTTGCATGTGCACTTTTAACTGCATTATATTAAAAGGAAAATTTGCGCGGTGACATCAGCAAAAGAACATTTTCCATTATTCTGAAAAGGTAGGTCAAATTTTAGTGTCATCCGTACAAATTTTCCATATTAAAAATGCACTCGCCACTTTCACTAATGGTCATGCTAGTTTGAGAAGGACAACATAGATGATGGAAAGTGGATCACAAAATGAGTAGATGATAAATATTATCCCTTTAAATAGGTAAAGAAGTTGGTTGTATATTTGTACTATGCTAATAATGCTCTCAAATATTTTCTTAAAAAATAATATTTTCATATTTTTAATAATTTAAAAACTGAAGTAGACCCTGTAGTTGTCTAGATTTATTTGTCATTCTTTTTTTCTTTTGAAAAGATTTATTTTTCATTCTTTTAAATTAACTATAAGTTACGAAATTTAAAATGTCAGTTAATTAAATTAGAATATGATATTTGAATACGAAAAAAAAAAAGAATAATACAAATCAGGAAGGGCTGAGAGAAAAAAAATCAGCCCAATCAATCCACCAAAGCCCAATAACTCAACTCAGCCTATCAAATAAATTAAAAATAAACAAAAGAAAGAGGAAAACCTAGTAACGTACCTACCCTTCATCTCTCACGTGCTCCTCCTTCGCCGTTCTCCCCATCCCTCTCTCTCAAGTCTCACTCTTCTCGCCGTCGCCGACGCCGGCGCCGGCTCCCCCTTCCTTTTAGCAGCTCTCGCCGCCCCGCCATTTATCTCCTGCCCGTGCCGCCGCCGCCGTCCCTCTCCTGATCGTGCTGACGCCGCCGCCTTCCTCCTAGCAGTTTGCACTGTTTTTCTATCTCTTGTTACTGGTATGCTCTCAATCTCCATCTATGTCTTTCTTCTCGCTTCCCGATTGAATCGACCGACTAGAACAACTCAATTAGGACTTGCGAATGAAAGTGATGTGGAGTTGCTTGACAGAACAGCCTCACCATTGATGAGGTTGTAGATTAAAATCATGGCAGTGAAGAAGACGAATTGATGCGTTTCAAATAAATCTATGTTAATTCTGAATACAATTGACACGTGAATGTAAATGTTAAACTGACGAAATTAAAAGCGCAACCTAAAGTTTGAAAATTTTCTTCTTTGGTATCTCATCCAGATTCAGTTTCTTGCCTGCCGTCGGAATGGCGAAGGAGGAATTTAGGGTTTACAATTCGATGACGCAGCAGAAGGAAATCTTCACGCCGAAGGAGCCTGGTAAGGTTGGCATGTACGTCTGTGGCATCACGGCCTATGATTTCAGCCACATTGGTCATGCTCGCGCCGCCGTCAACTTTGACGTCCTCTACAGGTTTGCTGATTTCTGCATTCGTATCTCTATACGCAAGTGA

mRNA sequence

ATGCTGACCTTTCTTTCCAGTTCAGTCATTCTTTCTTCGCTTGTACCTATGTCGACCATCATTACAGACATCACGTCAGGATCTGATGTCTCCTTTGCCTGCTCAGAAGATGAAAGGCTTGTTCTTCTTCTGATTTCTGGATTTCCTTGGAGCGTCCGCGATCTCACTCTTCTCGCCGTCGCCGACGCCGGCGCCGGCTCCCCCTTCCTTTTAGCAGCTCTCGCCGCCCCGCCATTTATCTCCTGCCCGTGCCGCCGCCGCCGTCCCTCTCCTGATCGTGCTGACGCCGCCGCCTTCCTCCTAGCAGTTTGCACTGTTTTTCTATCTCTTGTTACTGGTATGCTCTCAATCTCCATCTATGTCTTTCTTCTCGCTTCCCGATTGAATCGACCGACTAGAACAACTCAATTAGGACTTGCGAATGAAAGTGATGTGGAGTTGCTTGACAGAACAGCCTCACCATTGATGAGATTCAGTTTCTTGCCTGCCGTCGGAATGGCGAAGGAGGAATTTAGGGTTTACAATTCGATGACGCAGCAGAAGGAAATCTTCACGCCGAAGGAGCCTGGTAAGGTTGGCATGTACGTCTGTGGCATCACGGCCTATGATTTCAGCCACATTGGTCATGCTCGCGCCGCCGTCAACTTTGACGTCCTCTACAGGTTTGCTGATTTCTGCATTCGTATCTCTATACGCAAGTGA

Coding sequence (CDS)

ATGCTGACCTTTCTTTCCAGTTCAGTCATTCTTTCTTCGCTTGTACCTATGTCGACCATCATTACAGACATCACGTCAGGATCTGATGTCTCCTTTGCCTGCTCAGAAGATGAAAGGCTTGTTCTTCTTCTGATTTCTGGATTTCCTTGGAGCGTCCGCGATCTCACTCTTCTCGCCGTCGCCGACGCCGGCGCCGGCTCCCCCTTCCTTTTAGCAGCTCTCGCCGCCCCGCCATTTATCTCCTGCCCGTGCCGCCGCCGCCGTCCCTCTCCTGATCGTGCTGACGCCGCCGCCTTCCTCCTAGCAGTTTGCACTGTTTTTCTATCTCTTGTTACTGGTATGCTCTCAATCTCCATCTATGTCTTTCTTCTCGCTTCCCGATTGAATCGACCGACTAGAACAACTCAATTAGGACTTGCGAATGAAAGTGATGTGGAGTTGCTTGACAGAACAGCCTCACCATTGATGAGATTCAGTTTCTTGCCTGCCGTCGGAATGGCGAAGGAGGAATTTAGGGTTTACAATTCGATGACGCAGCAGAAGGAAATCTTCACGCCGAAGGAGCCTGGTAAGGTTGGCATGTACGTCTGTGGCATCACGGCCTATGATTTCAGCCACATTGGTCATGCTCGCGCCGCCGTCAACTTTGACGTCCTCTACAGGTTTGCTGATTTCTGCATTCGTATCTCTATACGCAAGTGA

Protein sequence

MLTFLSSSVILSSLVPMSTIITDITSGSDVSFACSEDERLVLLLISGFPWSVRDLTLLAVADAGAGSPFLLAALAAPPFISCPCRRRRPSPDRADAAAFLLAVCTVFLSLVTGMLSISIYVFLLASRLNRPTRTTQLGLANESDVELLDRTASPLMRFSFLPAVGMAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRFADFCIRISIRK
Homology
[Close]
BLAST of Spg020825.1 vs. NCBI nr
Match: XP_038907150.1 (cysteine--tRNA ligase 2, cytoplasmic [Benincasa hispida])

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-23
Identity = 55/57 (96.49%), Postives = 57/57 (100.00%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. NCBI nr
Match: XP_022152314.1 (cysteine--tRNA ligase 2, cytoplasmic [Momordica charantia])

HSP 1 Score: 118.6 bits (296), Expect = 7.1e-23
Identity = 54/60 (90.00%), Postives = 57/60 (95.00%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRFADF 225
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+  +
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRYLKY 60

[Close]
BLAST of Spg020825.1 vs. NCBI nr
Match: KGN51154.2 (hypothetical protein Csa_023165 [Cucumis sativus])

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-22
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. NCBI nr
Match: XP_008465226.2 (PREDICTED: LOW QUALITY PROTEIN: cysteine--tRNA ligase 2, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-22
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. NCBI nr
Match: XP_004150192.2 (LOW QUALITY PROTEIN: cysteine--tRNA ligase 2, cytoplasmic [Cucumis sativus])

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-22
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match: B3LFA4 (Cysteine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g38830 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 8.4e-19
Identity = 41/55 (74.55%), Postives = 49/55 (89.09%), Query Frame = 0 

Query: 168 KEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           K E ++YN+MTQQKE+  P  PGK+G+YVCGITAYDFSHIGHARAAV+FDVLYR+
Sbjct: 5   KMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRY 59

[Close]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match: Q9LYL3 (Cysteine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g56300 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.7e-17
Identity = 37/55 (67.27%), Postives = 48/55 (87.27%), Query Frame = 0 

Query: 168 KEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           K +  +YN+MTQ KE++ P  PGK+G+YVCGITAYD+SHIGHARAAV+FD+LYR+
Sbjct: 8   KPDLTLYNTMTQLKEVYKPMNPGKIGIYVCGITAYDYSHIGHARAAVSFDLLYRY 62

[Close]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match: Q3A8C9 (Cysteine--tRNA ligase OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=cysS PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 6.6e-16
Identity = 36/54 (66.67%), Postives = 44/54 (81.48%), Query Frame = 0 

Query: 172 RVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRFADF 226
           RVYN+MT +KE F P +PGKVGMYVCG+T YD+ HIGHARA V FD++YR+  F
Sbjct: 4   RVYNTMTGRKEEFEPLQPGKVGMYVCGVTVYDYCHIGHARANVVFDIIYRYLQF 57

[Close]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match: Q72BQ5 (Cysteine--tRNA ligase OS=Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) OX=882 GN=cysS PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.3e-15
Identity = 35/51 (68.63%), Postives = 44/51 (86.27%), Query Frame = 0 

Query: 172 RVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           ++YNS++++KE+FTP  PGKV MYVCGITAYD  HIGHAR+AV FDVL R+
Sbjct: 2   QIYNSLSRRKELFTPAVPGKVNMYVCGITAYDLCHIGHARSAVVFDVLVRY 52

[Close]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match: A1VDQ5 (Cysteine--tRNA ligase OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) OX=391774 GN=cysS PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.3e-15
Identity = 35/51 (68.63%), Postives = 44/51 (86.27%), Query Frame = 0 

Query: 172 RVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           ++YNS++++KE+FTP  PGKV MYVCGITAYD  HIGHAR+AV FDVL R+
Sbjct: 2   QIYNSLSRRKELFTPAVPGKVNMYVCGITAYDLCHIGHARSAVVFDVLVRY 52

[Close]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match: A0A6J1DFN5 (Cysteinyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020061 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 3.4e-23
Identity = 54/60 (90.00%), Postives = 57/60 (95.00%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRFADF 225
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+  +
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRYLKY 60

[Close]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match: A0A5D3CTE4 (Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00410 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 5.8e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match: A0A6J1I7Y5 (Cysteinyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111472181 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 5.8e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match: A0A6J1EQN5 (Cysteinyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111436590 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 5.8e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match: A0A1S3CNS9 (Cysteinyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502879 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 5.8e-23
Identity = 54/57 (94.74%), Postives = 56/57 (98.25%), Query Frame = 0 

Query: 166 MAKEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           MAKEEFRVYNSMTQQKE+FT KEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYR+
Sbjct: 1   MAKEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRY 57

[Close]
BLAST of Spg020825.1 vs. TAIR 10
Match: AT5G38830.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 95.5 bits (236), Expect = 6.0e-20
Identity = 41/55 (74.55%), Postives = 49/55 (89.09%), Query Frame = 0 

Query: 168 KEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           K E ++YN+MTQQKE+  P  PGK+G+YVCGITAYDFSHIGHARAAV+FDVLYR+
Sbjct: 5   KMELKLYNTMTQQKEVLIPITPGKIGLYVCGITAYDFSHIGHARAAVSFDVLYRY 59

[Close]
BLAST of Spg020825.1 vs. TAIR 10
Match: AT3G56300.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-18
Identity = 37/55 (67.27%), Postives = 48/55 (87.27%), Query Frame = 0 

Query: 168 KEEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           K +  +YN+MTQ KE++ P  PGK+G+YVCGITAYD+SHIGHARAAV+FD+LYR+
Sbjct: 8   KPDLTLYNTMTQLKEVYKPMNPGKIGIYVCGITAYDYSHIGHARAAVSFDLLYRY 62

[Close]
BLAST of Spg020825.1 vs. TAIR 10
Match: AT2G31170.1 (Cysteinyl-tRNA synthetase, class Ia family protein )

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-15
Identity = 34/54 (62.96%), Postives = 44/54 (81.48%), Query Frame = 0 

Query: 169 EEFRVYNSMTQQKEIFTPKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRF 223
           +E  ++N+M+++KE+F PK  GKVGMYVCG+TAYD SHIGHAR  V FDVL R+
Sbjct: 63  KELWLHNTMSRKKELFKPKVEGKVGMYVCGVTAYDLSHIGHARVYVTFDVLLRY 116

The following BLAST results are available for this feature:
BLAST of Spg020825.1 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038907150.11.4e-2396.49cysteine--tRNA ligase 2, cytoplasmic [Benincasa hispida][more]
XP_022152314.17.1e-2390.00cysteine--tRNA ligase 2, cytoplasmic [Momordica charantia][more]
KGN51154.21.2e-2294.74hypothetical protein Csa_023165 [Cucumis sativus][more]
XP_008465226.21.2e-2294.74PREDICTED: LOW QUALITY PROTEIN: cysteine--tRNA ligase 2, cytoplasmic-like [Cucum... [more]
XP_004150192.21.2e-2294.74LOW QUALITY PROTEIN: cysteine--tRNA ligase 2, cytoplasmic [Cucumis sativus][more]
BLAST of Spg020825.1 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
B3LFA48.4e-1974.55Cysteine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g3883... [more]
Q9LYL32.7e-1767.27Cysteine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g5630... [more]
Q3A8C96.6e-1666.67Cysteine--tRNA ligase OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 ... [more]
Q72BQ57.3e-1568.63Cysteine--tRNA ligase OS=Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / N... [more]
A1VDQ57.3e-1568.63Cysteine--tRNA ligase OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) OX=... [more]
BLAST of Spg020825.1 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DFN53.4e-2390.00Cysteinyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020061 PE=3 SV... [more]
A0A5D3CTE45.8e-2394.74Cysteinyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1I7Y55.8e-2394.74Cysteinyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111472181 PE=3 SV=1[more]
A0A6J1EQN55.8e-2394.74Cysteinyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111436590 PE=3 SV=... [more]
A0A1S3CNS95.8e-2394.74Cysteinyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502879 PE=3 SV=1[more]
BLAST of Spg020825.1 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G38830.16.0e-2074.55Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT3G56300.11.9e-1867.27Cysteinyl-tRNA synthetase, class Ia family protein [more]
AT2G31170.13.4e-1562.96Cysteinyl-tRNA synthetase, class Ia family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032678tRNA synthetases class I, catalytic domainPFAMPF01406tRNA-synt_1ecoord: 184..222
e-value: 7.7E-11
score: 41.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 156..227
e-value: 4.6E-18
score: 67.0
IPR024909Cysteinyl-tRNA synthetase/mycothiol ligasePANTHERPTHR10890CYSTEINYL-TRNA SYNTHETASEcoord: 168..222
NoneNo IPR availablePANTHERPTHR10890:SF29BNAA05G14150D PROTEINcoord: 168..222
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 170..223

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg020825Spg020825gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg020825.1-cdsSpg020825.1-cds-scaffold10:28411338..28411569CDS
Spg020825.1-cdsSpg020825.1-cds-scaffold10:28411733..28412041CDS
Spg020825.1-cdsSpg020825.1-cds-scaffold10:28416234..28416280CDS
Spg020825.1-cdsSpg020825.1-cds-scaffold10:28416372..28416485CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg020825.1Spg020825.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006423 cysteinyl-tRNA aminoacylation
biological_process GO:0016310 phosphorylation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0004817 cysteine-tRNA ligase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding