Spg018633.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg018633.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold3: 10403927 .. 10406493 (-)
Sequence length465
RNA-Seq ExpressionSpg018633.1
SyntenySpg018633.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTACCGAATGGAAAAGCTAATGTTGGGAATGACCCTAAGGGACCCTATCCATTTCTGGAGATTACTTTTGTAACTCGAGACCTACCTTGGATTATTTCCTCTTGTCATCTATTGAGGAAGTTGAACTAAAAGTTGTTGTTATTCATTGTTGAGGAAAAATGGCAAAGTTTTGTGGTTCTTTGTGGTTTCATTGTTGGTTTAAGTTGTTGGGATTTACTAAGTTAAAAGAAGAAAGAAAAGAAAAGAAAAGGAAAAGGAAAAGGGAAATGGAAGAAAAAAAAAAGGAAACAAATCAGCCAAAGGGAAATGAAAACAAAAAAAAAAAAAAAAAGAAAAAGAAAAGAAAGGAAATGGGAAATGACAGCAAACATTCCACCACCGACAATGATCCTTTTCAGCTCATTTCTAGCCATTTTCTCATCATTTTCCATCCAAAATTTCAGAGTACTTGGGGGAGAGCGTGAAGGAGGAAGAAGAAGAAGATTTTCGTGGTTTTGAGGTTGAAGAAGATAGGAAAAGCTCATGCAAAGCCAGCCATAGCAGAATCTGAGTTCAACCTGTCAACCTCTGGTTTTTTATTCAATTTGAGCTACACAGAAGGAACTAAGAGGTGAGGTTTGATTTTATTGAAAGTTATTTCATTTGCTAACAAATTTCATGAAGGAAGCTTGAGCTAAATCGTATGAGAAGTTAATGGTTTTTAAAATGGAAAACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGGTTTTTCGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTTATATGGTTGGAAATCTTATTCAATTTCGTACAACTTTCATGAAGAATGCGTGAGCCAAAAATGACTTGAAGTAGGTTTAAATTCAGGAGATAGATAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCGTTTCTGGACAAATTTGTTGAGAATCTCGTTTTCATTTCTTCAGGTAAGTTGTAGAGTGTTCTAAAATGAAGAGTTTGATGTATATTACATTAATTTTGGTTAAATATTGAGGAAGTTATGGGTGTTGGAAGGTGTGGTATTGAATCTGGAAAATCTGGAAAAGTAGAATGTGAAGTGTGATTTTTAATTCAAGTATTTAGAATTCATGAGCATGGAAGAATAAGACTATCTATTGTTCTAATGCTCGGTTTTGGTTGTTTGACAGGCCAAGAGGAAATAGGTCGAGTCTACAAACCAAGGAACTAGCTAAGACTATGAGTGACAAAATGAATTTCGAAAATTGTTTCCAACGGTTGTTCATGTTTAGACATTTTACATGCTTTTCTATGAATGATACATGATTTTTATGAATGGTTTCAAGCTTAAGTTATGAGCTTATATTTTCTAATGAGGTTTATAAATGAGCACGTGATTATTGAGTTTCTGAAAAATAGTTGACACGACATCCTTTTAAGCAAAGCATGTATTAGCAAGAATTATTTAAAGATTTTCGGTTTTTTTTTTTCACGAACAAGCTGAGTAAGCTTAAGTTTTACGAAAGATAACGATAAGCAGGCATGTTTTAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTGAGTGTACTCCGCGACAACGATGCTATCGTGAGTGCTGGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACATTTGATGTACTTGATATGCCTTGCTAGATTTTTAATGACGAACTTGCGATGTTTTAAGAAAGCTTTTATCATTGCACGTTTATGTTTAACGCCTTGGTTGAGCAGCTTTATACGTTTAAAGTTTTATCACGTGTTCTGATTTCCGAATCTTCAGTTTTAAATGTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTCCCACTTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACATCTGCTGAAAGTTCCAGTTCAAGCTCCAGTATGTGGGTGGAGTTGTATATTGAGTCTGTCCATATTGTAAATGTTTTGTAAGGATTGGATAATTTAGTCCTAGAGGTCGTAGTTGTATAAACCCCAGTTGTATACGTTTTTGGTGGTGATATCTATATCAGGTTATTTTCCAAACCGAGTTATGTCAGGGCGGCACCTCGTGTATGTTAAAAGGTTTTTCAGGGATTCCGCTGTGTTGTGTTTCACGTAGAATATTTTATATCTAAGATTAGTTCGTTTATCAGGTTCAATGTTACAGGAGAGTCAACAGGTATTGTTAGAGGAGGACGATGTTTGTTGGCTTCACGCCATCTTCCGGACTGAGTTAGCAGGTGGTCCGGGAGGGGGTGTGACAACTTTGTTTCGATTCCTATGTTACAACCCTCCGAATGGTTTAGTACCTCCAGGGCATACTTAG

mRNA sequence

ATGCTACCGAATGGAAAAGCTAATGTTGGGAATGACCCTAAGGGACCCTATCCATTTCTGGAGATTACTTTTAAGATAGGAAAAGCTCATGCAAAGCCAGCCATAGCAGAATCTGAGTTCAACCTGTCAACCTCTGGTTTTTTATTCAATTTGAGCTACACAGAAGGAACTAAGAGGTTATATTCCGTTGTTGACGTGAGTGTACTCCGCGACAACGATGCTATCGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACATCTGCTGAAAGTTCCAGTTCAAGCTCCAGTTCAATGTTACAGGAGAGTCAACAGGTATTGTTAGAGGAGGACGATGTTTGTTGGCTTCACGCCATCTTCCGGACTGAGTTAGCAGGTGGTCCGGGAGGGGGTGTGACAACTTTGTTTCGATTCCTATGTTACAACCCTCCGAATGGTTTAGTACCTCCAGGGCATACTTAG

Coding sequence (CDS)

ATGCTACCGAATGGAAAAGCTAATGTTGGGAATGACCCTAAGGGACCCTATCCATTTCTGGAGATTACTTTTAAGATAGGAAAAGCTCATGCAAAGCCAGCCATAGCAGAATCTGAGTTCAACCTGTCAACCTCTGGTTTTTTATTCAATTTGAGCTACACAGAAGGAACTAAGAGGTTATATTCCGTTGTTGACGTGAGTGTACTCCGCGACAACGATGCTATCGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACATCTGCTGAAAGTTCCAGTTCAAGCTCCAGTTCAATGTTACAGGAGAGTCAACAGGTATTGTTAGAGGAGGACGATGTTTGTTGGCTTCACGCCATCTTCCGGACTGAGTTAGCAGGTGGTCCGGGAGGGGGTGTGACAACTTTGTTTCGATTCCTATGTTACAACCCTCCGAATGGTTTAGTACCTCCAGGGCATACTTAG

Protein sequence

MLPNGKANVGNDPKGPYPFLEITFKIGKAHAKPAIAESEFNLSTSGFLFNLSYTEGTKRLYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQESQQVLLEEDDVCWLHAIFRTELAGGPGGGVTTLFRFLCYNPPNGLVPPGHT
Homology
BLAST of Spg018633.1 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 2.5e-16
Identity = 52/87 (59.77%), Postives = 65/87 (74.71%), Query Frame = 0

Query: 57  TKRLYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV------ 116
           T+ ++ +  +SVLRDNDA+VEIELPVPDTLPTSAESS S+SS+ L+   E   V      
Sbjct: 15  TQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLA 74

Query: 117 -LLEEDDVCWLHAIFRTELAGGPGGGV 134
            ++ EDDVCWLHA+FR + AGGPGGGV
Sbjct: 75  GIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg018633.1 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 2.5e-16
Identity = 52/87 (59.77%), Postives = 65/87 (74.71%), Query Frame = 0

Query: 57  TKRLYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV------ 116
           T+ ++ +  +SVLRDNDA+VEIELPVPDTLPTSAESS S+SS+ L+   E   V      
Sbjct: 15  TQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLA 74

Query: 117 -LLEEDDVCWLHAIFRTELAGGPGGGV 134
            ++ EDDVCWLHA+FR + AGGPGGGV
Sbjct: 75  GIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg018633.1 vs. NCBI nr
Match: KAA0060199.1 (uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ95571.1 uncharacterized protein E5676_scaffold767G00770 [Cucumis melo var. makuwa])

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-15
Identity = 48/75 (64.00%), Postives = 58/75 (77.33%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQESQQVLLEEDDVCWLHA 119
           ++S+  +SVLRDNDA+VEIELPVPDTLPTSAES  S+S S        ++  DDVCWLHA
Sbjct: 72  IFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRS------TGIVRGDDVCWLHA 131

Query: 120 IFRTELAGGPGGGVT 135
           +FR + AGGPGGGVT
Sbjct: 132 VFRAKTAGGPGGGVT 140

BLAST of Spg018633.1 vs. NCBI nr
Match: KAA0042035.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-15
Identity = 49/84 (58.33%), Postives = 63/84 (75.00%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV-------LL 119
           ++S+  +SVLRDND +VEIELP+PDTLPTSAESS S+SS++L+   ES  V       ++
Sbjct: 140 IFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLELYFESVHVEMFGSVGII 199

Query: 120 EEDDVCWLHAIFRTELAGGPGGGV 134
             DDVCWLHA+FR +  GGPGGGV
Sbjct: 200 RGDDVCWLHAVFRAKTTGGPGGGV 223

BLAST of Spg018633.1 vs. NCBI nr
Match: TYK17971.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-15
Identity = 49/84 (58.33%), Postives = 63/84 (75.00%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV-------LL 119
           ++S+  +SVLRDND +VEIELP+PDTLPTSAESS S+SS++L+   ES  V       ++
Sbjct: 140 IFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLELYFESVHVEMFGSVGII 199

Query: 120 EEDDVCWLHAIFRTELAGGPGGGV 134
             DDVCWLHA+FR +  GGPGGGV
Sbjct: 200 RGDDVCWLHAVFRAKTTGGPGGGV 223

BLAST of Spg018633.1 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-16
Identity = 52/87 (59.77%), Postives = 65/87 (74.71%), Query Frame = 0

Query: 57  TKRLYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV------ 116
           T+ ++ +  +SVLRDNDA+VEIELPVPDTLPTSAESS S+SS+ L+   E   V      
Sbjct: 15  TQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLA 74

Query: 117 -LLEEDDVCWLHAIFRTELAGGPGGGV 134
            ++ EDDVCWLHA+FR + AGGPGGGV
Sbjct: 75  GIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg018633.1 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-16
Identity = 52/87 (59.77%), Postives = 65/87 (74.71%), Query Frame = 0

Query: 57  TKRLYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV------ 116
           T+ ++ +  +SVLRDNDA+VEIELPVPDTLPTSAESS S+SS+ L+   E   V      
Sbjct: 15  TQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLA 74

Query: 117 -LLEEDDVCWLHAIFRTELAGGPGGGV 134
            ++ EDDVCWLHA+FR + AGGPGGGV
Sbjct: 75  GIVREDDVCWLHAVFRAKTAGGPGGGV 101

BLAST of Spg018633.1 vs. ExPASy TrEMBL
Match: A0A5D3BAD1 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00770 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.0e-16
Identity = 48/75 (64.00%), Postives = 58/75 (77.33%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQESQQVLLEEDDVCWLHA 119
           ++S+  +SVLRDNDA+VEIELPVPDTLPTSAES  S+S S        ++  DDVCWLHA
Sbjct: 72  IFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRS------TGIVRGDDVCWLHA 131

Query: 120 IFRTELAGGPGGGVT 135
           +FR + AGGPGGGVT
Sbjct: 132 VFRAKTAGGPGGGVT 140

BLAST of Spg018633.1 vs. ExPASy TrEMBL
Match: A0A5A7TKT0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G004880 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 7.8e-16
Identity = 49/84 (58.33%), Postives = 63/84 (75.00%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV-------LL 119
           ++S+  +SVLRDND +VEIELP+PDTLPTSAESS S+SS++L+   ES  V       ++
Sbjct: 140 IFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLELYFESVHVEMFGSVGII 199

Query: 120 EEDDVCWLHAIFRTELAGGPGGGV 134
             DDVCWLHA+FR +  GGPGGGV
Sbjct: 200 RGDDVCWLHAVFRAKTTGGPGGGV 223

BLAST of Spg018633.1 vs. ExPASy TrEMBL
Match: A0A5D3D150 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002770 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 7.8e-16
Identity = 49/84 (58.33%), Postives = 63/84 (75.00%), Query Frame = 0

Query: 60  LYSVVDVSVLRDNDAIVEIELPVPDTLPTSAESSSSSSSSMLQ---ESQQV-------LL 119
           ++S+  +SVLRDND +VEIELP+PDTLPTSAESS S+SS++L+   ES  V       ++
Sbjct: 140 IFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLELYFESVHVEMFGSVGII 199

Query: 120 EEDDVCWLHAIFRTELAGGPGGGV 134
             DDVCWLHA+FR +  GGPGGGV
Sbjct: 200 RGDDVCWLHAVFRAKTTGGPGGGV 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK27998.12.5e-1659.77reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0066851.12.5e-1659.77reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0060199.11.2e-1564.00uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ9... [more]
KAA0042035.11.6e-1558.33DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
TYK17971.11.6e-1558.33DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3DX391.2e-1659.77Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7VMG91.2e-1659.77Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3BAD16.0e-1664.00Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7TKT07.8e-1658.33Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67... [more]
A0A5D3D1507.8e-1658.33Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold30... [more]
Match NameE-valueIdentityDescription
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg018633Spg018633gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg018633.1-cdsSpg018633.1-cds-scaffold3:10403927..10404099CDS
Spg018633.1-cdsSpg018633.1-cds-scaffold3:10404356..10404422CDS
Spg018633.1-cdsSpg018633.1-cds-scaffold3:10404778..10404826CDS
Spg018633.1-cdsSpg018633.1-cds-scaffold3:10405881..10405984CDS
Spg018633.1-cdsSpg018633.1-cds-scaffold3:10406422..10406493CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg018633.1Spg018633.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
molecular_function GO:0005488 binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016779 nucleotidyltransferase activity