Spg016210.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg016210.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description28 kDa ribonucleoprotein, chloroplastic-like
Locationscaffold9: 42259347 .. 42261312 (+)
Sequence length936
RNA-Seq ExpressionSpg016210.1
SyntenySpg016210.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGTCCTCAAACTATACTAATACACGTCTCATGCCAGAATTCGCTAAAAATATCCATATGTTCTATTCGAAACGTTGACGTTTTAAGCCTTTTCCGAGCTTCCTCTTATCCAAATCCATGGCGTAGCTCTGAATTTGCTTTCAAACTAGAAGAGAGTGAACAGAGTGCGGTGAGCGCCGGCAATGGCTCTGGTTCGTCTTCCTTGTTCTTCATCTTCCACATTCCTCCGCTCTAAACAACAGCACCCAATCTTTCTCGCCCATCTCTCATGCCCGCAAAATCCTTCTTATTCCATCAATGGCGACATCAATCTCTCTCTTTCTCGCTCTTCTCCCTCTCTTCTTTCTACTTCCCTCAGCCACAATCCTTCAATTTCTGTTAGGGCTAAGAGAACTGCCAAGTTAGTTTTCCAGTTCGCCTCAACCTCCCAAGACGAAGCGGTAGCGAGCTCTCCTTCTGATACTGAGGAGTTTTCTCGAACCAGATTACTTGCTCAGAACGTGCCTTGGAATTCTACTCCCGAGGACATTCGTTCTCTGTTTGAGAAATATGGTACTGTCTTGGACGTGGAGGTATGTTCCTCTAGTTTATTTCTGTGAAAGAACATTTCTCTTGCCATTTCTGTTTGACGGTTGTGGTATTTCGTGAATTGATGATCGGTCTCTTTCTCTCAGCTTTCAATGTATAACAAGATTAGAAACAGGGGCTTGGCGTTTGTCACAATGGGATCACCTGAGGAGGCTCTTGCTGCATTTAATAATCTCGAATCGTATGTAAGCAGAATGATGTTTTTCTTAATTATAATATTTTCGTTTGGCTTATTGTGTTCAGTTTCGTAATTAAGGCTTAAAGCAATGTTCAGTGTTTATCCTTCATCATGGAAGTATAATTGTATTAGCAATCAAATGTGTTTAACTTAAGGAATAGTTTGGTTTGGCGTTGTTGGTCACTAGACCTTTTTGTAATTATGGTCTTGGTCTTGTTCTCAATTGGAGTAGTTAGTGTTAGACTTTTCTTTTAGGCTTGGTCTCGTTTCTTTTGGACTTTCTTTCTTTCGTTTTTTTAATGCAAGTTCAGTTGGTTTGCATGTCTGATGGACAAACTGATGAAGCAGAAAAACTAGGTATAAATTTGTATCCTGCTAGTGTCTCAATGTGTTGCAGTTGCTTTCACTTTTGCTTCTTTTATGCTTTTCAATATATTATTACAAGGGAAACAGTTATTGACTAAAGATTTTCAGTTCACCTTGTTACTTTGGGTTGAAGCAAAGTAGATTGTCTTTCCATTCATTTCAAAACTTCAACGATTCCTTTCCAAACCAAACATGAATCTTCTAATTCATTTGGCTCTCATGCTCAATCATTGCAATCATTTCTGGGAAATTCCATATGTCTTTTTGAACTCTTGTGTGCTTTATTTAATGCAATGGTTCACATAAATGCAGGAATTTGAGGGTCGTACTCTAAAGCTCAATTATGCCAAGCTTAAAAAAGAGAAACCCTCCCCTCCCGTGAAACCGAAACCAGTTACTTTTAATTTGTTTGTGGCAAATTTACCATTTGAAGCAAGAGCAAAGGACCTTAAGGAGTTCTTTGATTCAGGGAGTGGTAATGTAGTTTCTGCACAAATTATTTTTCATGAGAATCCGAGAAGGTCCTCTGGATATGGTTTTGTTGCCTTCAAAACCAAGAAGGATGCTGAAGCAGCTATTTCTGAGTTCCAAGGGAAGGTAATTTCAGTACTGCTACTACCTTATTTTTAAAAAACTGAATTAACCATGGAATCATCAAGTGTGAATTTCAGCATTCTTATGCTCCTTCATCTGTTGAAGTGCAGATTTTCATGGGGAGAGCACTTCGTGTAGCACGTAGCAAGCAGTTTGTGAAACTTCCTGAAGAGAAATCCCCATCTGAAGTTACATCAACTGATGGTGTGGAGCTAGCTAATGCTGCTGCTGAAGTTTAA

mRNA sequence

ATGGCTCTGGTTCGTCTTCCTTGTTCTTCATCTTCCACATTCCTCCGCTCTAAACAACAGCACCCAATCTTTCTCGCCCATCTCTCATGCCCGCAAAATCCTTCTTATTCCATCAATGGCGACATCAATCTCTCTCTTTCTCGCTCTTCTCCCTCTCTTCTTTCTACTTCCCTCAGCCACAATCCTTCAATTTCTGTTAGGGCTAAGAGAACTGCCAAGTTAGTTTTCCAGTTCGCCTCAACCTCCCAAGACGAAGCGGTAGCGAGCTCTCCTTCTGATACTGAGGAGTTTTCTCGAACCAGATTACTTGCTCAGAACGTGCCTTGGAATTCTACTCCCGAGGACATTCGTTCTCTGTTTGAGAAATATGGTACTGTCTTGGACGTGGAGCTTTCAATGTATAACAAGATTAGAAACAGGGGCTTGGCGTTTGTCACAATGGGATCACCTGAGGAGGCTCTTGCTGCATTTAATAATCTCGAATCGTATGAATTTGAGGGTCGTACTCTAAAGCTCAATTATGCCAAGCTTAAAAAAGAGAAACCCTCCCCTCCCGTGAAACCGAAACCAGTTACTTTTAATTTGTTTGTGGCAAATTTACCATTTGAAGCAAGAGCAAAGGACCTTAAGGAGTTCTTTGATTCAGGGAGTGGTAATGTAGTTTCTGCACAAATTATTTTTCATGAGAATCCGAGAAGGTCCTCTGGATATGGTTTTGTTGCCTTCAAAACCAAGAAGGATGCTGAAGCAGCTATTTCTGAGTTCCAAGGGAAGCATTCTTATGCTCCTTCATCTGTTGAAGTGCAGATTTTCATGGGGAGAGCACTTCGTGTAGCACGTAGCAAGCAGTTTGTGAAACTTCCTGAAGAGAAATCCCCATCTGAAGTTACATCAACTGATGGTGTGGAGCTAGCTAATGCTGCTGCTGAAGTTTAA

Coding sequence (CDS)

ATGGCTCTGGTTCGTCTTCCTTGTTCTTCATCTTCCACATTCCTCCGCTCTAAACAACAGCACCCAATCTTTCTCGCCCATCTCTCATGCCCGCAAAATCCTTCTTATTCCATCAATGGCGACATCAATCTCTCTCTTTCTCGCTCTTCTCCCTCTCTTCTTTCTACTTCCCTCAGCCACAATCCTTCAATTTCTGTTAGGGCTAAGAGAACTGCCAAGTTAGTTTTCCAGTTCGCCTCAACCTCCCAAGACGAAGCGGTAGCGAGCTCTCCTTCTGATACTGAGGAGTTTTCTCGAACCAGATTACTTGCTCAGAACGTGCCTTGGAATTCTACTCCCGAGGACATTCGTTCTCTGTTTGAGAAATATGGTACTGTCTTGGACGTGGAGCTTTCAATGTATAACAAGATTAGAAACAGGGGCTTGGCGTTTGTCACAATGGGATCACCTGAGGAGGCTCTTGCTGCATTTAATAATCTCGAATCGTATGAATTTGAGGGTCGTACTCTAAAGCTCAATTATGCCAAGCTTAAAAAAGAGAAACCCTCCCCTCCCGTGAAACCGAAACCAGTTACTTTTAATTTGTTTGTGGCAAATTTACCATTTGAAGCAAGAGCAAAGGACCTTAAGGAGTTCTTTGATTCAGGGAGTGGTAATGTAGTTTCTGCACAAATTATTTTTCATGAGAATCCGAGAAGGTCCTCTGGATATGGTTTTGTTGCCTTCAAAACCAAGAAGGATGCTGAAGCAGCTATTTCTGAGTTCCAAGGGAAGCATTCTTATGCTCCTTCATCTGTTGAAGTGCAGATTTTCATGGGGAGAGCACTTCGTGTAGCACGTAGCAAGCAGTTTGTGAAACTTCCTGAAGAGAAATCCCCATCTGAAGTTACATCAACTGATGGTGTGGAGCTAGCTAATGCTGCTGCTGAAGTTTAA

Protein sequence

MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSHNPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKEKPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLPEEKSPSEVTSTDGVELANAAAEV
Homology
BLAST of Spg016210.1 vs. NCBI nr
Match: XP_038897887.1 (31 kDa ribonucleoprotein, chloroplastic [Benincasa hispida])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 276/312 (88.46%), Postives = 287/312 (91.99%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MALVRLPCSSSS+FLRSKQQH IFLA LSCPQNPSY INGD NLS+SRSSPSLLSTS+ H
Sbjct: 1   MALVRLPCSSSSSFLRSKQQHLIFLAPLSCPQNPSYPINGDFNLSISRSSPSLLSTSIGH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP IS+R KRT+K VFQFASTSQDEAVASSPSD+EEFS+TRLLAQNVPW+STPEDIRSLF
Sbjct: 61  NPLISIRGKRTSKFVFQFASTSQDEAVASSPSDSEEFSQTRLLAQNVPWSSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPFEARAKDL+EFFDSGSGNVVSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLREFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFKTKKDAEAAISEFQGK           IFMGR+LRVARSKQFVKLP EEKS SE  ST
Sbjct: 241 AFKTKKDAEAAISEFQGK-----------IFMGRSLRVARSKQFVKLPSEEKSQSEDAST 300

Query: 301 DGVELANAAAEV 312
           D VELANAAAEV
Sbjct: 301 DDVELANAAAEV 301

BLAST of Spg016210.1 vs. NCBI nr
Match: XP_008466190.1 (PREDICTED: 31 kDa ribonucleoprotein, chloroplastic [Cucumis melo] >KAA0038685.1 31 kDa ribonucleoprotein [Cucumis melo var. makuwa] >TYK31295.1 31 kDa ribonucleoprotein [Cucumis melo var. makuwa])

HSP 1 Score: 508.1 bits (1307), Expect = 5.5e-140
Identity = 271/312 (86.86%), Postives = 281/312 (90.06%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MA VRLPCSSSS+ LRSKQ H IFLA LSC QNPSY INGD NLS+SRSSPSLLSTS+ H
Sbjct: 1   MASVRLPCSSSSSILRSKQHHHIFLAPLSCSQNPSYPINGDFNLSVSRSSPSLLSTSICH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP IS+ AKRT+K VFQFASTSQDEAV+SSPSD EEFSRTRLLAQNVPW+STPEDIRSLF
Sbjct: 61  NPLISIMAKRTSKFVFQFASTSQDEAVSSSPSDIEEFSRTRLLAQNVPWDSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPFEARAKDL+EFFDSGSGNVVSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLREFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFK KKDAEAAISEFQGK            FMGR+LRVARSKQFVKLP EEKS SE  ST
Sbjct: 241 AFKAKKDAEAAISEFQGK-----------TFMGRSLRVARSKQFVKLPSEEKSQSEDAST 300

Query: 301 DGVELANAAAEV 312
           DGVELANAAAEV
Sbjct: 301 DGVELANAAAEV 301

BLAST of Spg016210.1 vs. NCBI nr
Match: XP_022940500.1 (RNA-binding protein CP33, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 503.8 bits (1296), Expect = 1.0e-138
Identity = 267/311 (85.85%), Postives = 279/311 (89.71%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MALVRLPCSSSSTFLRSKQQHPIFLA LSC QNPS SINGDINLSL RSS SLLSTS  H
Sbjct: 1   MALVRLPCSSSSTFLRSKQQHPIFLAPLSCSQNPSSSINGDINLSLYRSSSSLLSTSFDH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP I     ++ +L+F FASTSQD+AVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF
Sbjct: 61  NPFIFTEGNKSRRLIFHFASTSQDDAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EK+GTVLDVELSMYNKIRNRGLAFVTM SPEEALAA NNLESYEFEGRTLKLNYAKLKKE
Sbjct: 121 EKFGTVLDVELSMYNKIRNRGLAFVTMRSPEEALAALNNLESYEFEGRTLKLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSP VKPKPVTFNLFVANL FEARAKDL+EFFDSGSGN+VSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPTVKPKPVTFNLFVANLSFEARAKDLREFFDSGSGNLVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLPEEKSPSEVTSTD 300
           AFKTK+DAEAA+SEFQGK           IFMGRALRVARSKQFVKLPEEKS SEVTSTD
Sbjct: 241 AFKTKRDAEAALSEFQGK-----------IFMGRALRVARSKQFVKLPEEKSQSEVTSTD 300

Query: 301 GVELANAAAEV 312
           G+ L+NAAAEV
Sbjct: 301 GMVLSNAAAEV 300

BLAST of Spg016210.1 vs. NCBI nr
Match: XP_022940495.1 (RNA-binding protein CP33, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022940497.1 RNA-binding protein CP33, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022940498.1 RNA-binding protein CP33, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022940499.1 RNA-binding protein CP33, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 271/329 (82.37%), Postives = 286/329 (86.93%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MALVRLPCSSSSTFLRSKQQHPIFLA LSC QNPS SINGDINLSL RSS SLLSTS  H
Sbjct: 1   MALVRLPCSSSSTFLRSKQQHPIFLAPLSCSQNPSSSINGDINLSLYRSSSSLLSTSFDH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP I     ++ +L+F FASTSQD+AVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF
Sbjct: 61  NPFIFTEGNKSRRLIFHFASTSQDDAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EK+GTVLDVELSMYNKIRNRGLAFVTM SPEEALAA NNLESYEFEGRTLKLNYAKLKKE
Sbjct: 121 EKFGTVLDVELSMYNKIRNRGLAFVTMRSPEEALAALNNLESYEFEGRTLKLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSP VKPKPVTFNLFVANL FEARAKDL+EFFDSGSGN+VSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPTVKPKPVTFNLFVANLSFEARAKDLREFFDSGSGNLVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGK-------HSYAPSSV-----------EVQIFMGRALRVARSK 300
           AFKTK+DAEAA+SEFQGK       +  A  +V           E+QIFMGRALRVARSK
Sbjct: 241 AFKTKRDAEAALSEFQGKVISGVTTYFLASRTVYISILMVLHLFELQIFMGRALRVARSK 300

Query: 301 QFVKLPEEKSPSEVTSTDGVELANAAAEV 312
           QFVKLPEEKS SEVTSTDG+ L+NAAAEV
Sbjct: 301 QFVKLPEEKSQSEVTSTDGMVLSNAAAEV 329

BLAST of Spg016210.1 vs. NCBI nr
Match: XP_004136268.1 (28 kDa ribonucleoprotein, chloroplastic [Cucumis sativus] >KGN60238.1 hypothetical protein Csa_002107 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 1.5e-137
Identity = 269/312 (86.22%), Postives = 281/312 (90.06%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MA VRLPCSSSS+ LRSK  H IFLA LSC QNPSY INGD NLS+SRSSPSLLSTS+SH
Sbjct: 1   MASVRLPCSSSSSILRSKHHHQIFLAPLSCSQNPSYPINGDFNLSISRSSPSLLSTSISH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           +P IS+RAKRT+  VFQFASTSQDEAV SSPSDTEEFS+TRLLAQNVPW+STPEDIRSLF
Sbjct: 61  SPLISIRAKRTSTFVFQFASTSQDEAV-SSPSDTEEFSQTRLLAQNVPWDSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPF+ARAKDLKEFFDSGSGNVVSAQIIF+ENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFDARAKDLKEFFDSGSGNVVSAQIIFNENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFKTKKDAEAAISEFQGK            FMGR+LRVARSKQFVKLP EEK  SE  ST
Sbjct: 241 AFKTKKDAEAAISEFQGK-----------TFMGRSLRVARSKQFVKLPSEEKPQSEDAST 300

Query: 301 DGVELANAAAEV 312
           DGVELANAAAEV
Sbjct: 301 DGVELANAAAEV 300

BLAST of Spg016210.1 vs. ExPASy Swiss-Prot
Match: Q39061 (RNA-binding protein CP33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP33 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.3e-18
Identity = 70/226 (30.97%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 101 RLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEALAAFN 160
           RL   N+P+  T  ++  +F + GTV+DV++ +Y+K+  R+RG  FVTMGS EEA  A  
Sbjct: 117 RLYVGNLPYTITSSELSQIFGEAGTVVDVQI-VYDKVTDRSRGFGFVTMGSIEEAKEAMQ 176

Query: 161 NLESYEFEGRTLKLNYAKLKKEKPSPPVKPKPVTFN---------LFVANLPFEARAKDL 220
              S +  GRT+K+N+ ++ +   +  ++ K    N         ++  NL +   ++ L
Sbjct: 177 MFNSSQIGGRTVKVNFPEVPRGGENEVMRTKIRDNNRSYVDSPHKVYAGNLGWNLTSQGL 236

Query: 221 KEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQ 280
           K+ F    G V+ A++I+  N  RS G+GF++F++ ++ ++A++   G        VEV+
Sbjct: 237 KDAFGDQPG-VLGAKVIYERNTGRSRGFGFISFESAENVQSALATMNG--------VEVE 296

Query: 281 IFMGRALRV----ARSKQFVKLP--EEKSPSEVTSTDGVELANAAA 310
              GRALR+     R K  V  P  EE    E +      L+N +A
Sbjct: 297 ---GRALRLNLASEREKPTVSPPSVEEGETEEASLESNEVLSNVSA 329

BLAST of Spg016210.1 vs. ExPASy Swiss-Prot
Match: P19684 (33 kDa ribonucleoprotein, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.5e-15
Identity = 80/276 (28.99%), Postives = 135/276 (48.91%), Query Frame = 0

Query: 45  SLSRSSPSLLSTSLSHNPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLA 104
           SL RSS   LST  S +  + V  +   + V   A   ++        ++E     RL  
Sbjct: 60  SLYRSS-IFLSTCASVSDGVEVVQEDDEEEVALSAEEEEEIEEKEERVESESVEGGRLYV 119

Query: 105 QNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEALAAFNNLES 164
            N+P++ T   +  +F + GTV +VE+ +Y+++  R+RG AFVTMGS EEA  A    + 
Sbjct: 120 GNLPFSMTSSQLSEIFAEAGTVANVEI-VYDRVTDRSRGFAFVTMGSVEEAKEAIRLFDG 179

Query: 165 YEFEGRTLKLNYAKL----KKEKPSPPVKPKPVTF-----NLFVANLPFEARAKDLKEFF 224
            +  GRT+K+N+ ++    ++E  S  ++     F      L+VANL +   ++ L++ F
Sbjct: 180 SQVGGRTVKVNFPEVPRGGEREVMSAKIRSTYQGFVDSPHKLYVANLSWALTSQGLRDAF 239

Query: 225 DSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMG 284
               G  +SA++I+  +  RS G+GF+ F + +   +A+           + VE++   G
Sbjct: 240 ADQPG-FMSAKVIYDRSSGRSRGFGFITFSSAEAMNSALDTM--------NEVELE---G 299

Query: 285 RALR--VARSKQFVKLPEEKSPSEVTSTDGVELANA 308
           R LR  VA  K  V  P     S    +D  EL ++
Sbjct: 300 RPLRLNVAGQKAPVSSPPVVETSPENDSDNSELLSS 321

BLAST of Spg016210.1 vs. ExPASy Swiss-Prot
Match: P49314 (31 kDa ribonucleoprotein, chloroplastic OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.3e-14
Identity = 74/247 (29.96%), Postives = 112/247 (45.34%), Query Frame = 0

Query: 39  NGDINLSLSRSSPSLLSTSLSHNPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFS 98
           +  +NLSLS SS   +  S S  P  S  + R A  +  F     D  VA  P  +E+  
Sbjct: 35  SSSLNLSLSSSS---IGHSASIKPFESSFSTRVA--LSDFDQLEDDVEVAEQPRFSEDL- 94

Query: 99  RTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEALAA 158
             +L   N+P++     +  LFE+ G V  VE+ +Y+K+  R+RG  FVTM + EE  AA
Sbjct: 95  --KLFVGNLPFSVDSAALAGLFERAGNVEIVEV-IYDKLSGRSRGFGFVTMSTKEEVEAA 154

Query: 159 FNNLESYEFEGRTLKLNY--AKLKKEKPS------------------------PPVKPKP 218
                 YE +GR +++N   A  K+E  S                           +   
Sbjct: 155 EQQFNGYEIDGRAIRVNAGPAPAKRENSSFGGGRGGNSSYGGGRDGNSSFGGARGGRSVD 214

Query: 219 VTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEA 258
            +  ++V NL +      LKE F S  GNVV A++++  +  RS G+GFV + + K+   
Sbjct: 215 SSNRVYVGNLSWGVDDLALKELF-SEQGNVVDAKVVYDRDSGRSRGFGFVTYSSAKEVND 271

BLAST of Spg016210.1 vs. ExPASy Swiss-Prot
Match: Q08937 (29 kDa ribonucleoprotein B, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-13
Identity = 78/273 (28.57%), Postives = 122/273 (44.69%), Query Frame = 0

Query: 39  NGDINLSLSRSSPSLLSTSLSHNPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFS 98
           +  +NLSLS SS  L S       S S R   +      F     D  VA  P  +E+  
Sbjct: 35  SSSLNLSLSSSSTGLCSIK-PFESSFSTRVALSG-----FDQLEDDVEVAEQPRFSEDL- 94

Query: 99  RTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEALAA 158
             +L   N+P++     +  LFE+ G V  VE+ +Y+K+  R+RG  FVTM + EE  AA
Sbjct: 95  --KLFVGNLPFSVDSAALAGLFERAGNVEMVEV-IYDKLTGRSRGFGFVTMSTKEEVEAA 154

Query: 159 FNNLESYEFEGRTLKLNY--AKLKKEKPS------------------------PPVKPKP 218
                 YE +GR +++N   A  K+E  S                           +   
Sbjct: 155 EQQFNGYEIDGRAIRVNAGPAPAKRENSSFGGGRGGNSSYGGGRDGNSSFGGARGGRSVD 214

Query: 219 VTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEA 278
            +  ++V NL +      LKE F S  GNVV A++++  +  RS G+GFV + + K+   
Sbjct: 215 SSNRVYVGNLSWGVDDLALKELF-SEQGNVVDAKVVYDRDSGRSRGFGFVTYSSSKEVND 274

Query: 279 AISEFQGKHSYAPSSVEVQIFMGRALRVARSKQ 284
           AI    G        V++    GR++RV+ +++
Sbjct: 275 AIDSLNG--------VDLD---GRSIRVSAAEE 285

BLAST of Spg016210.1 vs. ExPASy Swiss-Prot
Match: Q9ZUU4 (RNA-binding protein CP29B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP29B PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 69/249 (27.71%), Postives = 104/249 (41.77%), Query Frame = 0

Query: 45  SLSRSSPSLLSTSLSHNPSISVRAK---------RTAKLVFQFASTSQDEAVASSPSDTE 104
           S+S  SPSL     S + S S+ AK         R   +  +F       A  + P +  
Sbjct: 27  SVSLLSPSLSFKLNSDSVSFSIAAKWNSPASRFARNVAITSEFEVEEDGFADVAPPKEQS 86

Query: 105 EFSRTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEA 164
             +  +L   N+P+N     +  LFE  G V  VE+ +Y+KI  R+RG  FVTM S  E 
Sbjct: 87  FSADLKLFVGNLPFNVDSAQLAQLFESAGNVEMVEV-IYDKITGRSRGFGFVTMSSVSEV 146

Query: 165 LAAFNNLESYEFEGRTLKLNYAKLKKEKPSPPVKPKPVT--------------------- 224
            AA      YE +GR L++N        P PP +    +                     
Sbjct: 147 EAAAQQFNGYELDGRPLRVNAG------PPPPKREDGFSRGPRSSFGSSGSGYGGGGGSG 206

Query: 225 ----FNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDA 258
                 ++V NL +      L+  F S  G VV A++I+  +  RS G+GFV + + ++ 
Sbjct: 207 AGSGNRVYVGNLSWGVDDMALESLF-SEQGKVVEARVIYDRDSGRSKGFGFVTYDSSQEV 266

BLAST of Spg016210.1 vs. ExPASy TrEMBL
Match: A0A5A7TBD1 (31 kDa ribonucleoprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005930 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.7e-140
Identity = 271/312 (86.86%), Postives = 281/312 (90.06%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MA VRLPCSSSS+ LRSKQ H IFLA LSC QNPSY INGD NLS+SRSSPSLLSTS+ H
Sbjct: 1   MASVRLPCSSSSSILRSKQHHHIFLAPLSCSQNPSYPINGDFNLSVSRSSPSLLSTSICH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP IS+ AKRT+K VFQFASTSQDEAV+SSPSD EEFSRTRLLAQNVPW+STPEDIRSLF
Sbjct: 61  NPLISIMAKRTSKFVFQFASTSQDEAVSSSPSDIEEFSRTRLLAQNVPWDSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPFEARAKDL+EFFDSGSGNVVSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLREFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFK KKDAEAAISEFQGK            FMGR+LRVARSKQFVKLP EEKS SE  ST
Sbjct: 241 AFKAKKDAEAAISEFQGK-----------TFMGRSLRVARSKQFVKLPSEEKSQSEDAST 300

Query: 301 DGVELANAAAEV 312
           DGVELANAAAEV
Sbjct: 301 DGVELANAAAEV 301

BLAST of Spg016210.1 vs. ExPASy TrEMBL
Match: A0A1S3CS17 (31 kDa ribonucleoprotein, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503681 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.7e-140
Identity = 271/312 (86.86%), Postives = 281/312 (90.06%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MA VRLPCSSSS+ LRSKQ H IFLA LSC QNPSY INGD NLS+SRSSPSLLSTS+ H
Sbjct: 1   MASVRLPCSSSSSILRSKQHHHIFLAPLSCSQNPSYPINGDFNLSVSRSSPSLLSTSICH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP IS+ AKRT+K VFQFASTSQDEAV+SSPSD EEFSRTRLLAQNVPW+STPEDIRSLF
Sbjct: 61  NPLISIMAKRTSKFVFQFASTSQDEAVSSSPSDIEEFSRTRLLAQNVPWDSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPFEARAKDL+EFFDSGSGNVVSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLREFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFK KKDAEAAISEFQGK            FMGR+LRVARSKQFVKLP EEKS SE  ST
Sbjct: 241 AFKAKKDAEAAISEFQGK-----------TFMGRSLRVARSKQFVKLPSEEKSQSEDAST 300

Query: 301 DGVELANAAAEV 312
           DGVELANAAAEV
Sbjct: 301 DGVELANAAAEV 301

BLAST of Spg016210.1 vs. ExPASy TrEMBL
Match: A0A6J1FQT2 (RNA-binding protein CP33, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446072 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.0e-139
Identity = 267/311 (85.85%), Postives = 279/311 (89.71%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MALVRLPCSSSSTFLRSKQQHPIFLA LSC QNPS SINGDINLSL RSS SLLSTS  H
Sbjct: 1   MALVRLPCSSSSTFLRSKQQHPIFLAPLSCSQNPSSSINGDINLSLYRSSSSLLSTSFDH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP I     ++ +L+F FASTSQD+AVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF
Sbjct: 61  NPFIFTEGNKSRRLIFHFASTSQDDAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EK+GTVLDVELSMYNKIRNRGLAFVTM SPEEALAA NNLESYEFEGRTLKLNYAKLKKE
Sbjct: 121 EKFGTVLDVELSMYNKIRNRGLAFVTMRSPEEALAALNNLESYEFEGRTLKLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSP VKPKPVTFNLFVANL FEARAKDL+EFFDSGSGN+VSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPTVKPKPVTFNLFVANLSFEARAKDLREFFDSGSGNLVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLPEEKSPSEVTSTD 300
           AFKTK+DAEAA+SEFQGK           IFMGRALRVARSKQFVKLPEEKS SEVTSTD
Sbjct: 241 AFKTKRDAEAALSEFQGK-----------IFMGRALRVARSKQFVKLPEEKSQSEVTSTD 300

Query: 301 GVELANAAAEV 312
           G+ L+NAAAEV
Sbjct: 301 GMVLSNAAAEV 300

BLAST of Spg016210.1 vs. ExPASy TrEMBL
Match: A0A6J1FKE6 (RNA-binding protein CP33, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446072 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.6e-139
Identity = 271/329 (82.37%), Postives = 286/329 (86.93%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MALVRLPCSSSSTFLRSKQQHPIFLA LSC QNPS SINGDINLSL RSS SLLSTS  H
Sbjct: 1   MALVRLPCSSSSTFLRSKQQHPIFLAPLSCSQNPSSSINGDINLSLYRSSSSLLSTSFDH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           NP I     ++ +L+F FASTSQD+AVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF
Sbjct: 61  NPFIFTEGNKSRRLIFHFASTSQDDAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EK+GTVLDVELSMYNKIRNRGLAFVTM SPEEALAA NNLESYEFEGRTLKLNYAKLKKE
Sbjct: 121 EKFGTVLDVELSMYNKIRNRGLAFVTMRSPEEALAALNNLESYEFEGRTLKLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSP VKPKPVTFNLFVANL FEARAKDL+EFFDSGSGN+VSAQIIFHENPRRSSGYGFV
Sbjct: 181 KPSPTVKPKPVTFNLFVANLSFEARAKDLREFFDSGSGNLVSAQIIFHENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGK-------HSYAPSSV-----------EVQIFMGRALRVARSK 300
           AFKTK+DAEAA+SEFQGK       +  A  +V           E+QIFMGRALRVARSK
Sbjct: 241 AFKTKRDAEAALSEFQGKVISGVTTYFLASRTVYISILMVLHLFELQIFMGRALRVARSK 300

Query: 301 QFVKLPEEKSPSEVTSTDGVELANAAAEV 312
           QFVKLPEEKS SEVTSTDG+ L+NAAAEV
Sbjct: 301 QFVKLPEEKSQSEVTSTDGMVLSNAAAEV 329

BLAST of Spg016210.1 vs. ExPASy TrEMBL
Match: A0A0A0LEN0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G889940 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 7.3e-138
Identity = 269/312 (86.22%), Postives = 281/312 (90.06%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MA VRLPCSSSS+ LRSK  H IFLA LSC QNPSY INGD NLS+SRSSPSLLSTS+SH
Sbjct: 1   MASVRLPCSSSSSILRSKHHHQIFLAPLSCSQNPSYPINGDFNLSISRSSPSLLSTSISH 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDTEEFSRTRLLAQNVPWNSTPEDIRSLF 120
           +P IS+RAKRT+  VFQFASTSQDEAV SSPSDTEEFS+TRLLAQNVPW+STPEDIRSLF
Sbjct: 61  SPLISIRAKRTSTFVFQFASTSQDEAV-SSPSDTEEFSQTRLLAQNVPWDSTPEDIRSLF 120

Query: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLNYAKLKKE 180
           EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAA NNLESYEFEGRTL+LNYAKLKKE
Sbjct: 121 EKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAALNNLESYEFEGRTLRLNYAKLKKE 180

Query: 181 KPSPPVKPKPVTFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFV 240
           KPSPPVKPKPVTFNLFVANLPF+ARAKDLKEFFDSGSGNVVSAQIIF+ENPRRSSGYGFV
Sbjct: 181 KPSPPVKPKPVTFNLFVANLPFDARAKDLKEFFDSGSGNVVSAQIIFNENPRRSSGYGFV 240

Query: 241 AFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKLP-EEKSPSEVTST 300
           AFKTKKDAEAAISEFQGK            FMGR+LRVARSKQFVKLP EEK  SE  ST
Sbjct: 241 AFKTKKDAEAAISEFQGK-----------TFMGRSLRVARSKQFVKLPSEEKPQSEDAST 300

Query: 301 DGVELANAAAEV 312
           DGVELANAAAEV
Sbjct: 301 DGVELANAAAEV 300

BLAST of Spg016210.1 vs. TAIR 10
Match: AT4G09040.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-71
Identity = 165/317 (52.05%), Postives = 208/317 (65.62%), Query Frame = 0

Query: 1   MALVRLPCSSSSTFLRSKQQHPIFLAHLSCPQNPSYSINGDINLSLSRSSPSLLSTSLSH 60
           MAL+RLP  S              L H S  +NP+ ++    N SLS S+PSL       
Sbjct: 1   MALLRLPGISLQ-----------ILGHKSNQKNPNPNV-AFTNHSLSLSTPSLCRLHRHA 60

Query: 61  NPSISVRAKRTAKLVFQFASTSQDEAVASSPSDT-------EEFSRTRLLAQNVPWNSTP 120
               S+ AK +  L   F++T+Q+  + SS S +       EE S+TRL+AQNVPW STP
Sbjct: 61  TFPDSIPAK-SRNLTSYFSTTTQEPVLESSSSSSSAPEVVEEEISKTRLIAQNVPWTSTP 120

Query: 121 EDIRSLFEKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAFNNLESYEFEGRTLKLN 180
           EDIRSLFEKYG+V+D+E+SM+ K RNRGL F+ M SPEEA  A  +LES E+EGR LK++
Sbjct: 121 EDIRSLFEKYGSVIDIEMSMHKKERNRGLVFIEMASPEEAATALKSLESCEYEGRRLKVD 180

Query: 181 YAKLKKEKP-SPPVKPKPV-TFNLFVANLPFEARAKDLKEFFDSGSGNVVSAQIIFHENP 240
           YAK KK+K  +P   P PV TFNLFVANL FEARAK LKEFFD+ +GNVVS ++IFHENP
Sbjct: 181 YAKTKKKKTYAPRETPSPVPTFNLFVANLAFEARAKHLKEFFDADTGNVVSTEVIFHENP 240

Query: 241 RRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRALRVARSKQFVKL---- 300
           RRSSGYGFV+FKTKK AEAA+ EFQGK            F+GR +R+A+SKQFVKL    
Sbjct: 241 RRSSGYGFVSFKTKKQAEAALIEFQGKD-----------FLGRPIRLAKSKQFVKLQAKE 293

BLAST of Spg016210.1 vs. TAIR 10
Match: AT4G09040.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.7e-67
Identity = 133/213 (62.44%), Postives = 162/213 (76.06%), Query Frame = 0

Query: 98  SRTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKIRNRGLAFVTMGSPEEALAAF 157
           S+TRL+AQNVPW STPEDIRSLFEKYG+V+D+E+SM+ K RNRGL F+ M SPEEA  A 
Sbjct: 32  SKTRLIAQNVPWTSTPEDIRSLFEKYGSVIDIEMSMHKKERNRGLVFIEMASPEEAATAL 91

Query: 158 NNLESYEFEGRTLKLNYAKLKKEKP-SPPVKPKPV-TFNLFVANLPFEARAKDLKEFFDS 217
            +LES E+EGR LK++YAK KK+K  +P   P PV TFNLFVANL FEARAK LKEFFD+
Sbjct: 92  KSLESCEYEGRRLKVDYAKTKKKKTYAPRETPSPVPTFNLFVANLAFEARAKHLKEFFDA 151

Query: 218 GSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQIFMGRA 277
            +GNVVS ++IFHENPRRSSGYGFV+FKTKK AEAA+ EFQGK            F+GR 
Sbjct: 152 DTGNVVSTEVIFHENPRRSSGYGFVSFKTKKQAEAALIEFQGKD-----------FLGRP 211

Query: 278 LRVARSKQFVKL--------PEEKSPSEVTSTD 301
           +R+A+SKQFVKL        PEE++  E + ++
Sbjct: 212 IRLAKSKQFVKLQAKEGLQPPEEEAEEEPSQSE 233

BLAST of Spg016210.1 vs. TAIR 10
Match: AT2G35410.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 122.9 bits (307), Expect = 4.7e-28
Identity = 94/286 (32.87%), Postives = 146/286 (51.05%), Query Frame = 0

Query: 47  SRSSPSLLSTSLS------HNPSISVR--AKRTAKLVFQFASTSQDEAVASSPSDTEEFS 106
           S+ S SL  T+LS       +P  S+R  +  + +LV    S ++ E  A   +  EE +
Sbjct: 26  SKRSSSLKLTALSLRIPSNLSPVFSLRSGSDNSRRLVSVLCSVAEKETSADEETSQEEKT 85

Query: 107 --------RTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKIRNRGLAFVTMGSP 166
                   + +L   N+PW+ +  DI  LF + GTV +VE+      +NRG AFVTM S 
Sbjct: 86  EETQNSNLKRKLFVFNLPWSMSVNDISELFGQCGTVNNVEIIRQKDGKNRGFAFVTMASG 145

Query: 167 EEALAAFNNLESYEFEGRTLKLNYA-KLKKEKPS-----PPVKPKPVTFNLFVANLPFEA 226
           EEA AA +  ++++  GR + +++A + KK  P      P   P      L+V+NL ++A
Sbjct: 146 EEAQAAIDKFDTFQVSGRIISVSFARRFKKPTPKSPNDLPSPAPGDTRHKLYVSNLAWKA 205

Query: 227 RAKDLKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPS 286
           R+  L+E F +   N VSA+++F +   RSSGYGFV+F T+++AE AI++  GK      
Sbjct: 206 RSTHLRELFTAADFNPVSARVVFADPEGRSSGYGFVSFATREEAENAITKLNGKE----- 265

Query: 287 SVEVQIFMGRALRVARSKQFVKLPEEKSPSEVTSTDGVELANAAAE 311
                  MGR + +  S         +S SE    D VE  NA+ +
Sbjct: 266 ------IMGRPITLKFSL--------RSASESEDGDSVEANNASED 292

BLAST of Spg016210.1 vs. TAIR 10
Match: AT3G52380.1 (chloroplast RNA-binding protein 33 )

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-19
Identity = 70/226 (30.97%), Postives = 119/226 (52.65%), Query Frame = 0

Query: 101 RLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSPEEALAAFN 160
           RL   N+P+  T  ++  +F + GTV+DV++ +Y+K+  R+RG  FVTMGS EEA  A  
Sbjct: 117 RLYVGNLPYTITSSELSQIFGEAGTVVDVQI-VYDKVTDRSRGFGFVTMGSIEEAKEAMQ 176

Query: 161 NLESYEFEGRTLKLNYAKLKKEKPSPPVKPKPVTFN---------LFVANLPFEARAKDL 220
              S +  GRT+K+N+ ++ +   +  ++ K    N         ++  NL +   ++ L
Sbjct: 177 MFNSSQIGGRTVKVNFPEVPRGGENEVMRTKIRDNNRSYVDSPHKVYAGNLGWNLTSQGL 236

Query: 221 KEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEVQ 280
           K+ F    G V+ A++I+  N  RS G+GF++F++ ++ ++A++   G        VEV+
Sbjct: 237 KDAFGDQPG-VLGAKVIYERNTGRSRGFGFISFESAENVQSALATMNG--------VEVE 296

Query: 281 IFMGRALRV----ARSKQFVKLP--EEKSPSEVTSTDGVELANAAA 310
              GRALR+     R K  V  P  EE    E +      L+N +A
Sbjct: 297 ---GRALRLNLASEREKPTVSPPSVEEGETEEASLESNEVLSNVSA 329

BLAST of Spg016210.1 vs. TAIR 10
Match: AT1G60000.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 82.8 bits (203), Expect = 5.3e-16
Identity = 75/258 (29.07%), Postives = 124/258 (48.06%), Query Frame = 0

Query: 33  NPSYSINGDINLSLSRSSPSLLSTSLSHNPSISVRAKRTAKLVFQFASTSQDEAVASSPS 92
           N  +  N   N +L R + S     L  + S S R +  ++ +       + +  AS+  
Sbjct: 18  NLLHGCNYSSNQTLLRFNFSTPKPLLISSRSCSRRFRVLSETITVKLEEEEKDDGASAVL 77

Query: 93  DTEEFSRTRLLAQNVPWNSTPEDIRSLFEKYGTVLDVELSMYNKI--RNRGLAFVTMGSP 152
           D      T+L   N+P+N     +  + + +     VE+ +YN+   ++RG AFVTM + 
Sbjct: 78  DPPAAVNTKLYFGNLPYNVDSATLAQIIQDFANPELVEV-LYNRDTGQSRGFAFVTMSNV 137

Query: 153 EEALAAFNNLESYEFEGRTLKLNYAKLKKEKPSPPVKP--KPVTFNLFVANLPFEARAKD 212
           E+     +NL+  E+ GR LK+N+A    +KP P  +P        LFV NL +   ++ 
Sbjct: 138 EDCNIIIDNLDGTEYLGRALKVNFA----DKPKPNKEPLYPETEHKLFVGNLSWTVTSES 197

Query: 213 LKEFFDSGSGNVVSAQIIFHENPRRSSGYGFVAFKTKKDAEAAISEFQGKHSYAPSSVEV 272
           L   F    G+VV A+++F  +  RS GYGFV + +K + E A+    G         E+
Sbjct: 198 LAGAFRE-CGDVVGARVVFDGDTGRSRGYGFVCYSSKAEMETALESLDG--------FEL 257

Query: 273 QIFMGRALRV--ARSKQF 285
           +   GRA+RV  A+ K+F
Sbjct: 258 E---GRAIRVNLAQGKKF 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897887.11.1e-14388.4631 kDa ribonucleoprotein, chloroplastic [Benincasa hispida][more]
XP_008466190.15.5e-14086.86PREDICTED: 31 kDa ribonucleoprotein, chloroplastic [Cucumis melo] >KAA0038685.1 ... [more]
XP_022940500.11.0e-13885.85RNA-binding protein CP33, chloroplastic-like isoform X2 [Cucurbita moschata][more]
XP_022940495.11.8e-13882.37RNA-binding protein CP33, chloroplastic-like isoform X1 [Cucurbita moschata] >XP... [more]
XP_004136268.11.5e-13786.2228 kDa ribonucleoprotein, chloroplastic [Cucumis sativus] >KGN60238.1 hypothetic... [more]
Match NameE-valueIdentityDescription
Q390614.3e-1830.97RNA-binding protein CP33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP33 ... [more]
P196847.5e-1528.9933 kDa ribonucleoprotein, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=1 SV=... [more]
P493148.3e-1429.9631 kDa ribonucleoprotein, chloroplastic OS=Nicotiana plumbaginifolia OX=4092 PE=... [more]
Q089371.1e-1328.5729 kDa ribonucleoprotein B, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 S... [more]
Q9ZUU42.0e-1227.71RNA-binding protein CP29B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CP29... [more]
Match NameE-valueIdentityDescription
A0A5A7TBD12.7e-14086.8631 kDa ribonucleoprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CS172.7e-14086.8631 kDa ribonucleoprotein, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503681 ... [more]
A0A6J1FQT25.0e-13985.85RNA-binding protein CP33, chloroplastic-like isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1FKE68.6e-13982.37RNA-binding protein CP33, chloroplastic-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A0A0LEN07.3e-13886.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G889940 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G09040.11.5e-7152.05RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G09040.28.7e-6762.44RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT2G35410.14.7e-2832.87RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT3G52380.13.0e-1930.97chloroplast RNA-binding protein 33 [more]
AT1G60000.15.3e-1629.07RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 101..173
e-value: 9.4E-16
score: 68.3
coord: 194..269
e-value: 5.0E-16
score: 69.2
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 195..258
e-value: 6.4E-15
score: 54.8
coord: 105..171
e-value: 3.3E-13
score: 49.3
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 100..177
score: 16.3978
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 193..283
score: 16.585409
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 181..286
e-value: 5.1E-20
score: 74.0
coord: 61..180
e-value: 1.2E-20
score: 76.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..311
NoneNo IPR availablePANTHERPTHR23003RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEINcoord: 1..298
NoneNo IPR availablePANTHERPTHR23003:SF35OS08G0483200 PROTEINcoord: 1..298
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 100..259

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg016210Spg016210gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg016210.1-five_prime_utrSpg016210.1-five_prime_utr-scaffold9:42259347..42259529five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg016210.1-cdsSpg016210.1-cds-scaffold9:42259530..42259919CDS
Spg016210.1-cdsSpg016210.1-cds-scaffold9:42260022..42260120CDS
Spg016210.1-cdsSpg016210.1-cds-scaffold9:42260792..42261076CDS
Spg016210.1-cdsSpg016210.1-cds-scaffold9:42261151..42261312CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg016210.1Spg016210.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
cellular_component GO:0009507 chloroplast
cellular_component GO:1990904 ribonucleoprotein complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003729 mRNA binding