Spg014073.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg014073.1 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 105.5 bits (262), Expect = 9.6e-19
Identity = 62/220 (28.18%), Postives = 100/220 (45.45%), Query Frame = 0

Query: 82  KKLEMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIWKADRGKELEEDR 141
           KK E   H+ WECK+++ +W+   P     F+ DR+ WT  +Y E  W  D+  E EE R
Sbjct: 132 KKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWE--WLMDKAGE-EERR 191

Query: 142 MARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYC-------SEFLRNEIPY----- 201
             RS+++  QIW  RN+ +      + + ++  I  Y        +   R    +     
Sbjct: 192 --RSMIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQDTNLKRKSKDFHPIRR 251

Query: 202 ----------------LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVS 261
                            +LN D  W       GIGWILR   G  +    + +R    ++
Sbjct: 252 IGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNIT 311

Query: 262 WLEVAAICEGLKIIPRD-SPLVRVESDAMNVVRLLNDEVQ 273
           +LEV AICEGL+ I ++    + +ESD++  + LL+  V+
Sbjct: 312 YLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 346

BLAST of Spg014073.1 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 102.4 bits (254), Expect = 8.1e-18
Identity = 77/218 (35.32%), Postives = 111/218 (50.92%), Query Frame = 0

Query: 146 LVVCWQIWNHRNEVL----HNKKQPDIQQLEQKIT--NYCSE--------FLRNEIPY-- 205
           L+  W IWNHRN V+    H+     IQQL + +T  +Y SE         L N++ +  
Sbjct: 14  LIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKLKWEP 73

Query: 206 -----LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAAICEGL 265
                  LN D +WS++  RGGIGWI+RS +G  + A  + V     V  LE +AI EGL
Sbjct: 74  PPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGL 133

Query: 266 KIIPRDSPL--VRVESDAMNVVRLLNDEVQDISELAIFVMEAKSLISA--IIPVEKVAYA 325
           + +     L  + +E+D+  V  LLN + +D+++    V E  +L  +  I+   KV   
Sbjct: 134 RNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVG-- 193

Query: 326 LRFHNGVAHYLAQQAVSLNSSEFWSNSFPNWFLVLNAS 339
            R  NG AH LAQ+A  L  S  W + FPNW  +L  S
Sbjct: 194 -RETNGCAHSLAQRASVLRESMIWVDCFPNWLSILAKS 228

BLAST of Spg014073.1 vs. NCBI nr
Match: XP_024046691.1 (uncharacterized protein LOC112101027 [Citrus clementina])

HSP 1 Score: 89.7 bits (221), Expect = 5.5e-14
Identity = 80/290 (27.59%), Postives = 124/290 (42.76%), Query Frame = 0

Query: 70  VDTKWLESTVLVKKLEMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIW 129
           V   W +   L K  E   H  +ECK  + +W +  PF  +I F        L   +G+ 
Sbjct: 2   VQLPWCQRCKLTK--ETVFHTLYECKASKKIW-RLTPFGEDIEFSKVR--DVLSLLQGLN 61

Query: 130 KADRGKELEEDRMARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYCSEFLRNEIP- 189
              R  E+E       +VVCW IW+ RN  L   K+ D Q            + R ++P 
Sbjct: 62  GKRRKSEME-----LIVVVCWSIWHSRNLFLFKNKKEDPQLSVAAAEAMVQSYSRIQMPQ 121

Query: 190 ---------------------YLELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVR 249
                                + ++N D      QQR G+G ++R+  G  + A+ K  +
Sbjct: 122 MQGNSQQSTANQKQWKHPPEGWFKVNVDAAVRVEQQRTGMGIVIRNSRGKVMAAAMKPSK 181

Query: 250 KRWKVSWLEVAAICEGLKIIP--RDSPLVRVESDAMNVVRLLNDEVQDISELAIFVMEAK 309
              KV + +  AI  GL+I    R  PL+ VESD+  VV L+N +    +E+     E +
Sbjct: 182 FIGKVDYAKAEAIRFGLEIAENVRCFPLI-VESDSQEVVDLINSKTSSRAEIFWVASEVQ 241

Query: 310 SLISAIIPVEKVAYALRFHNGVAHYLAQQAVSLNSSEFWSNSFPNWFLVL 336
             +  +  V KV +  R  N +AH LA+ A+      FW ++ P+ FL L
Sbjct: 242 DRMKRLNQV-KVQHTPRGCNTLAHSLARLALEQTDFVFWVDNIPSEFLYL 279

BLAST of Spg014073.1 vs. NCBI nr
Match: KAG6693248.1 (hypothetical protein I3842_10G159900, partial [Carya illinoinensis])

HSP 1 Score: 89.0 bits (219), Expect = 9.3e-14
Identity = 68/268 (25.37%), Postives = 117/268 (43.66%), Query Frame = 0

Query: 84  LEMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIWKADRGKELEEDRMA 143
           +E A H  + C  VR +W+ +CP   EI   D S W   +        DRG     D + 
Sbjct: 473 VEDAAHALFYCNDVRQVWMDYCPELGEI-VSDLSFWDLANLAR-----DRG---SHDVLT 532

Query: 144 RSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYCSEFLR-----------NEIPY--- 203
             LVV W++WN RN+ ++     +I            E+ +           N + Y   
Sbjct: 533 TFLVVAWRLWNRRNKFIYEDVSLNINTAVNNALPLKQEYTKVQVFHGFDKRINHVVYWHP 592

Query: 204 -----LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAAICEGL 263
                L+LN D          G+G ILR  NG  + A+ K  R+     ++E  A+  GL
Sbjct: 593 PPIGCLKLNVDGATFSDSFSAGVGVILRDHNGDVVVAASKVEREVSSAVFIEAIALLRGL 652

Query: 264 KI-IPRDSPLVRVESDAMNVVRLLNDEVQDISELAIFVMEAKSLISAIIPVEKVAYALRF 323
           ++ +    P + +ESD++ +V  LN++ + ++E    + + + L+       ++ +  R 
Sbjct: 653 QLCVQWGVPKIILESDSLILVNCLNEDSECLTEFDFILQDIRRLMHG-FEETRLEHVHRL 712

Query: 324 HNGVAHYLAQQAVSLNSSEFWSNSFPNW 332
            NG AH LA+ A  +     W +S P++
Sbjct: 713 GNGAAHQLARYAREVEDIVMWWDSCPSF 730

BLAST of Spg014073.1 vs. NCBI nr
Match: KAG6693249.1 (hypothetical protein I3842_10G159900 [Carya illinoinensis])

HSP 1 Score: 89.0 bits (219), Expect = 9.3e-14
Identity = 68/268 (25.37%), Postives = 117/268 (43.66%), Query Frame = 0

Query: 84  LEMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIWKADRGKELEEDRMA 143
           +E A H  + C  VR +W+ +CP   EI   D S W   +        DRG     D + 
Sbjct: 469 VEDAAHALFYCNDVRQVWMDYCPELGEI-VSDLSFWDLANLAR-----DRG---SHDVLT 528

Query: 144 RSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYCSEFLR-----------NEIPY--- 203
             LVV W++WN RN+ ++     +I            E+ +           N + Y   
Sbjct: 529 TFLVVAWRLWNRRNKFIYEDVSLNINTAVNNALPLKQEYTKVQVFHGFDKRINHVVYWHP 588

Query: 204 -----LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAAICEGL 263
                L+LN D          G+G ILR  NG  + A+ K  R+     ++E  A+  GL
Sbjct: 589 PPIGCLKLNVDGATFSDSFSAGVGVILRDHNGDVVVAASKVEREVSSAVFIEAIALLRGL 648

Query: 264 KI-IPRDSPLVRVESDAMNVVRLLNDEVQDISELAIFVMEAKSLISAIIPVEKVAYALRF 323
           ++ +    P + +ESD++ +V  LN++ + ++E    + + + L+       ++ +  R 
Sbjct: 649 QLCVQWGVPKIILESDSLILVNCLNEDSECLTEFDFILQDIRRLMHG-FEETRLEHVHRL 708

Query: 324 HNGVAHYLAQQAVSLNSSEFWSNSFPNW 332
            NG AH LA+ A  +     W +S P++
Sbjct: 709 GNGAAHQLARYAREVEDIVMWWDSCPSF 726

BLAST of Spg014073.1 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 4.7e-19
Identity = 62/220 (28.18%), Postives = 100/220 (45.45%), Query Frame = 0

Query: 82  KKLEMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIWKADRGKELEEDR 141
           KK E   H+ WECK+++ +W+   P     F+ DR+ WT  +Y E  W  D+  E EE R
Sbjct: 132 KKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWE--WLMDKAGE-EERR 191

Query: 142 MARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYC-------SEFLRNEIPY----- 201
             RS+++  QIW  RN+ +      + + ++  I  Y        +   R    +     
Sbjct: 192 --RSMIIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQDTNLKRKSKDFHPIRR 251

Query: 202 ----------------LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVS 261
                            +LN D  W       GIGWILR   G  +    + +R    ++
Sbjct: 252 IGDNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNIT 311

Query: 262 WLEVAAICEGLKIIPRD-SPLVRVESDAMNVVRLLNDEVQ 273
           +LEV AICEGL+ I ++    + +ESD++  + LL+  V+
Sbjct: 312 YLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 346

BLAST of Spg014073.1 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.9e-18
Identity = 77/218 (35.32%), Postives = 111/218 (50.92%), Query Frame = 0

Query: 146 LVVCWQIWNHRNEVL----HNKKQPDIQQLEQKIT--NYCSE--------FLRNEIPY-- 205
           L+  W IWNHRN V+    H+     IQQL + +T  +Y SE         L N++ +  
Sbjct: 14  LIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKLKWEP 73

Query: 206 -----LELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAAICEGL 265
                  LN D +WS++  RGGIGWI+RS +G  + A  + V     V  LE +AI EGL
Sbjct: 74  PPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGL 133

Query: 266 KIIPRDSPL--VRVESDAMNVVRLLNDEVQDISELAIFVMEAKSLISA--IIPVEKVAYA 325
           + +     L  + +E+D+  V  LLN + +D+++    V E  +L  +  I+   KV   
Sbjct: 134 RNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVG-- 193

Query: 326 LRFHNGVAHYLAQQAVSLNSSEFWSNSFPNWFLVLNAS 339
            R  NG AH LAQ+A  L  S  W + FPNW  +L  S
Sbjct: 194 -RETNGCAHSLAQRASVLRESMIWVDCFPNWLSILAKS 228

BLAST of Spg014073.1 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.8e-13
Identity = 56/209 (26.79%), Postives = 101/209 (48.33%), Query Frame = 0

Query: 139 EDRMARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKI-----------TNYCSEFLRNEI 198
           E+   RS+++ WQIW  RN+ +     P+ + ++  I           TN   +    ++
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDL 65

Query: 199 PYL-------------------ELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRK 258
             +                   +LN +  W      GGIGWILR   G  + AS + +R 
Sbjct: 66  HLIRRIEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRA 125

Query: 259 RWKVSWLEVAAICEGLKIIPRD-SPLVRVESDAMNVVRLLNDEVQDISELAIFVMEAKSL 317
              +++LEV AICEGL+ I ++    + +ESD++  + LL+ + QD +E+ I+++E    
Sbjct: 126 ERNITYLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEI-IWLLEEIWQ 185

BLAST of Spg014073.1 vs. ExPASy TrEMBL
Match: B8AFN2 (RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_06781 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.5e-13
Identity = 69/258 (26.74%), Postives = 115/258 (44.57%), Query Frame = 0

Query: 129 WKADRGKELEEDRMARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKITNYCS-------- 188
           W  D  ++L        L+  W+ W+ RNE+ H+K  P ++  ++ + +Y +        
Sbjct: 61  WLFDMLEQLPAHEHEMFLMTLWRNWHVRNEITHDKASPPLEVSKRFVESYIATLFQIKES 120

Query: 189 -----------------------------------EFLRNEIPYLELNCDTTWSETQQRG 248
                                               +++ ++ +++LN D ++     +G
Sbjct: 121 PLKDVEKGKSVASLSVTGRVRDRASDDHRVPNPVLHWVKPQVGWMKLNVDGSFDAALCKG 180

Query: 249 GIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAAICEGLKIIPRDSPL-VRVESDAMNVV 308
           GIG +LR  +G  + AS K + +       E+ A  EGL ++   + L + VE+D M+VV
Sbjct: 181 GIGMVLRDSSGNTIFASCKPLNRCTGALESELRACVEGLNLVLHWTLLPILVETDCMSVV 240

Query: 309 RLLNDEVQDISELAIFVMEAKSLISAI--IPVEKVAYALRFHNGVAHYLAQQAVSLNSSE 341
           +LLND  +D SELA  V EAK L++    I V K+    R  N V+H LA +A   + S 
Sbjct: 241 QLLNDGERDRSELANIVHEAKRLLAGTRRILVSKIH---RSQNSVSHLLANRAKVDSVSG 300

BLAST of Spg014073.1 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-11
Identity = 56/217 (25.81%), Postives = 101/217 (46.54%), Query Frame = 0

Query: 139 EDRMARSLVVCWQIWNHRNEVLHNKKQPDIQQLEQKI-----------TNYCSEFLRNEI 198
           E+   RS+++ WQIW  RN+ +      + + ++  I           TN   +    ++
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDL 65

Query: 199 PYL-------------------ELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRK 258
             +                   +LN D  W      GGIGWILR   G  + A  + +R 
Sbjct: 66  HLIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRT 125

Query: 259 RWKVSWLEVAAICEGLKIIPRD--SPL-------VRVESDAMNVVRLLNDEVQDISELAI 317
              +++LEV AICEGL+ I ++   P+       + +ESD++  + LL+ + QD +E+ I
Sbjct: 126 ERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI-I 185

BLAST of Spg014073.1 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-09
Identity = 61/274 (22.26%), Postives = 115/274 (41.97%), Query Frame = 0

Query: 85  EMANHLFWECKMVRGLWLKFCPFTNEIFFGDRSGWTPLDYCEGIWKADRGKELEEDRMAR 144
           E  NHL ++C   R  W       + I       W    Y    W  + G    +   A 
Sbjct: 303 ETVNHLLFKCTFARLTWA-----ISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKAS 362

Query: 145 SLV--VCWQIWNHRNEVLHNKKQPDIQQLEQKITNYCSEF-LRNEI-------------- 204
            LV  + W++W +RNE++   ++ + Q++ ++  +   E+ +R E               
Sbjct: 363 QLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSC 422

Query: 205 --------PYLELNCDTTWSETQQRGGIGWILRSPNGTPLCASYKCVRKRWKVSWLEVAA 264
                    +++ N D TW+   +R GIGW+LR+  G       + + K   V   E+ A
Sbjct: 423 GRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEA 482

Query: 265 ICEGLKIIPR-DSPLVRVESDAMNVVRLLNDEVQDISELAIFVMEAKSLISAIIPVEKVA 324
           +   +  + R     V  ESD+  ++ +LN++ +    L   + + + L+S    V K  
Sbjct: 483 MRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRLLSQFTEV-KFV 542

Query: 325 YALRFHNGVAHYLAQQAVS-LNSSEFWSNSFPNW 332
           +  R  N +A  +A++++S LN      +  P+W
Sbjct: 543 FIPREGNTLAERVARESLSFLNYDPKLYSIVPSW 569