Spg013457.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg013457.1 vs. NCBI nr
Match: XP_023518115.1 (nodulation-signaling pathway 2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.8 bits (2054), Expect = 2.1e-226
Identity = 412/498 (82.73%), Postives = 450/498 (90.36%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFF-QQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY++F QQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFSQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSVEEEEEEE--NSNNLFKGIQAE 120
                LDQFDP I Y  E+FL TTMDHQNI  MG+SRSV+E+EEEE  NS  +FKGIQAE
Sbjct: 64  -----LDQFDP-INYNFEEFLATTMDHQNIPEMGDSRSVQEDEEEEEGNSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFTQGLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVIANCSGILH  LSHRN SKLETFL+G++K+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGVYENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPCDKN  NL + AFD+LKGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  SLSL
Sbjct: 424 SVSQFPCDKNAMNLREKAFDYLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGSLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. NCBI nr
Match: XP_022962651.1 (nodulation-signaling pathway 2 protein-like [Cucurbita moschata])

HSP 1 Score: 793.5 bits (2048), Expect = 1.0e-225
Identity = 411/498 (82.53%), Postives = 449/498 (90.16%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFF-QQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY++F QQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFSQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP I Y  EDFL TTMDHQ+I  MG+SRSV  +EEEEEENS  +FKGIQAE
Sbjct: 64  -----LDQFDP-INYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFTQGLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVIANCSGILH  LSHRN SKLETFL+G++K+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGVYENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC+KN  NL + AFD+ KGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  SLSL
Sbjct: 424 SVSQFPCEKNAMNLREKAFDYFKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGSLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. NCBI nr
Match: KAG6595119.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.6 bits (2043), Expect = 4.0e-225
Identity = 410/498 (82.33%), Postives = 449/498 (90.16%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFF-QQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY++F QQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 1   MELEPIDFLTNYSYFSQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 60

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP I Y  EDFL TTMDHQ+I  MG+SRSV  +EEEEEENS  +FKGIQAE
Sbjct: 61  -----LDQFDP-INYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKGIQAE 120

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFT+GLHYK
Sbjct: 121 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTRGLHYK 180

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 181 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 240

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 241 DFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 300

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQ VIANCSGILH  LSHRN SKLETFL+G++K+SPKCVVL
Sbjct: 301 KFDHMGIEKDENFEQIQVLGQAVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCVVL 360

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGVYENGFKQVM+EFLGTRIL+
Sbjct: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRILE 420

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC+KN  NL + AFD+LKGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  SLSL
Sbjct: 421 SVSQFPCEKNAMNLREKAFDYLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGSLSL 480

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 481 CWKSRPLCSATIWVPTAK 491

BLAST of Spg013457.1 vs. NCBI nr
Match: KAG7027132.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 791.6 bits (2043), Expect = 4.0e-225
Identity = 410/498 (82.33%), Postives = 450/498 (90.36%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFF-QQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY++F QQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFSQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP I Y  EDFL TTMDHQ+I  MG+SRSV  +EEEEEENS  +FKGIQAE
Sbjct: 64  -----LDQFDP-INYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSF+QLAWFFT+GLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFSQLAWFFTRGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVIANCSGILH  LSHRN SKLETFL+G++K+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGVYENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC+KN  NL + AFD+LKGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  SLSL
Sbjct: 424 SVSQFPCEKNAMNLREKAFDYLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGSLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. NCBI nr
Match: XP_023003976.1 (nodulation-signaling pathway 2 protein-like [Cucurbita maxima])

HSP 1 Score: 789.3 bits (2037), Expect = 2.0e-224
Identity = 411/498 (82.53%), Postives = 447/498 (89.76%), Query Frame = 0

Query: 1   MELEPIEFLTNYT-FFQQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY+ FFQQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFFQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP   Y  EDFLTTTMDHQ+I  MG+SRSV  E+EEEEENS  +FKGIQAE
Sbjct: 64  -----LDQFDP-NNYNFEDFLTTTMDHQSIPEMGDSRSVQEEDEEEEENSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFTQGLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLM DLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMEDLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVI NCSGILH  LSHRN SKLETFL+GISK+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIGNCSGILHS-LSHRNSSKLETFLNGISKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGV+ENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVHENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC KN  NL + AFD+LKGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  +LSL
Sbjct: 424 SVSQFPCGKNAMNLREKAFDYLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGTLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.3e-48
Identity = 155/461 (33.62%), Postives = 232/461 (50.33%), Query Frame = 0

Query: 58  VEMLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSVEEEEEEENSNNLFKGIQAE 117
           +E     L+Q  P I  T     TTT D             EEEE E  +      I+  
Sbjct: 61  IEDRTTVLEQLSPSI--TTTTTTTTTTDE------------EEEEMETTTTTTTTAIKTH 120

Query: 118 LMEEES----LTDLLLAAAEAV--EAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFT 177
            + ++S    L  LL+A AEA+    +N+ L   ++ +LK L+      S+  +LA  FT
Sbjct: 121 EVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFT 180

Query: 178 Q----------GLHYKALEYNISAHEM-ADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTA 237
           +          G H     +N + H +  +      N++++AFQ+LQ +SPY+KF HFTA
Sbjct: 181 EALHGLLEGAGGAHNNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTA 240

Query: 238 NQAILEASEGEKLIHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENERKI-- 297
           NQAI+EA   E+ +HVID+DIMEG+QW  L+  LA+      LR+TA+ +     R I  
Sbjct: 241 NQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRRSIAT 300

Query: 298 -EQTGRRLSEFAKSMNFPFMFDQMGIEKEENFEQIQ---VMGQTVIANCSGILHHILSHR 357
            ++TGRRL+ FA S+  PF F    ++ +E F       V G+ ++ NC   L H LS+R
Sbjct: 301 VQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPH-LSYR 360

Query: 358 NLSKLETFLSGISKMSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCF 417
               + +FL+G   ++PK V LVEEE+  V       FVE F ++ HH+SA+ DSL   F
Sbjct: 361 APESVASFLNGAKTLNPKLVTLVEEEVGSVIG----GFVERFMDSLHHYSAVFDSLEAGF 420

Query: 418 SGVYENGFKQVMEE-FLGTRILDSVSQF--PCDKNVRNLWKNAFDHLKGYKRIPFSSFNC 477
               +N  + ++E  F G RI  S+ +      +  R  W      + G++ +P S  N 
Sbjct: 421 P--MQNRARTLVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEV-GFRGVPVSFANH 480

Query: 478 SQAKYLISLFRGDFWVQH---EKCSLSLCWKSRPLCTATIW 490
            QAK L+ LF   + V+        L L WKSR L +A++W
Sbjct: 481 CQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLW 499

BLAST of Spg013457.1 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.9e-44
Identity = 142/426 (33.33%), Postives = 223/426 (52.35%), Query Frame = 0

Query: 94  NSRSVEEEEEEENSNNLFKGIQAELMEEESLTD---------LLLAAAEAVEA--QNQPL 153
           ++ S EEEEEE  +  +           E+  D         LL+A AEA+    +N+ L
Sbjct: 76  STTSEEEEEEEATTTTMTTTTTTTTTTPEAADDDFKGLRLVHLLMAGAEALTGANKNREL 135

Query: 154 VSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKALE-----YNISA--HEMADQDQIK 213
              ++ +LK L+    G ++  +LA +FT+ L    LE     YN S+  H +       
Sbjct: 136 ARVILVRLKELVSHTDG-TNMERLAAYFTEALQ-GLLEGAGGAYNSSSKHHVIGGPHHEP 195

Query: 214 SNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVIDFDIMEGIQWPPLMADLA 273
            N++++AFQ+LQ +SPY+KF HFTANQAI+EA   E+ +H++D+DIMEG+QW  LM  LA
Sbjct: 196 QNDALAAFQLLQDMSPYVKFGHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQALA 255

Query: 274 AKKEVCLLRLTAIVQDSENERK---IEQTGRRLSEFAKSMNFPFMFDQMGIEKEENFEQI 333
           +      LR+TA+ +     R    +++TGRRL+ FA S+  PF F    +E +E F   
Sbjct: 256 SNPNGPHLRITALSRSGVGRRSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRPA 315

Query: 334 ---QVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVLVEEELFKVSKAQP 393
               V G+ ++ NC   L H L++R+ + + +FL+    + P+ V +VEEE+        
Sbjct: 316 GLKLVRGEALVFNCMLNLPH-LTYRSPNSVASFLTAAKALRPRLVTVVEEEVGSALG--- 375

Query: 394 MSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEE--FLGTRILDSVSQF---PCD 453
             FVE F ++ HHFSA+ DSL    +G    G  + + E  FLG RI+ S+++       
Sbjct: 376 -GFVERFMDSLHHFSAVFDSLE---AGFPMQGRARALVERVFLGPRIVGSLARIYRTGGG 435

Query: 454 KNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQH-EKCSLSLCWKSRPL 490
              R  W+  +    G+  +  SS N  Q+  L+ LF   + V+      L L WK+R L
Sbjct: 436 GEERGSWRE-WLRAAGFSGVAVSSANHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRL 490

BLAST of Spg013457.1 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.8e-40
Identity = 132/401 (32.92%), Postives = 206/401 (51.37%), Query Frame = 0

Query: 124 LTDLLLAAAEAVEA--QNQPLVSNLIEKLKNLL----LCDMGSSSFNQLAWFFT---QGL 183
           L  LL+AAAEA+    +++ L   ++ +LK ++      +  +S+  +LA  FT   QGL
Sbjct: 132 LLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERLAAHFTDALQGL 191

Query: 184 HYKALEYNISAHE-MADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKL 243
              +     S  +  A      + + ++AFQMLQ +SPY+KF HFTANQAILEA  G++ 
Sbjct: 192 LDGSHPVGGSGRQAAAAASHHHAGDVLTAFQMLQDMSPYMKFGHFTANQAILEAVSGDRR 251

Query: 244 IHVIDFDIMEGIQWPPLMADLAAKKE---VCLLRLTAIVQD-SENERKIEQTGRRLSEFA 303
           +H++D+DI EGIQW  LM  + ++ +      LR+TA+ +      R +++ GRRLS FA
Sbjct: 252 VHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRSGGGGARAVQEAGRRLSAFA 311

Query: 304 KSMNFPFMFDQMGIEKEENFEQI---QVMGQTVIANCSGILHHILS----HRNLSKLETF 363
            S+  PF F Q  ++ +E F       V G+ ++ANC  +LH   +     R    + +F
Sbjct: 312 ASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVANC--VLHQAAATTTIRRPTGSVASF 371

Query: 364 LSGISKMSPKCVVLVEEE--------LFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCF 423
           LSG++ +  K V +VEEE              A    FV  F E  H +SA+ DSL   F
Sbjct: 372 LSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGFVRQFMEELHRYSAVWDSLEAGF 431

Query: 424 SGVYENGFKQVMEE-FLGTRILDSVSQF--PCDKNVRNLWKNAFDHLKGYKRIPFSSFNC 483
               ++  + ++E   L   I  +VS+     D   R  W   +    G+  +P S FN 
Sbjct: 432 P--TQSRVRGLVERVILAPNIAGAVSRAYRGVDGEGRCGW-GQWMRGSGFTAVPLSCFNH 491

Query: 484 SQAKYLISLFRGDFWVQHE-KCSLSLCWKSRPLCTATIWVP 492
           SQA+ L+ LF   + V+      + L WK+R L +A++W P
Sbjct: 492 SQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVWAP 527

BLAST of Spg013457.1 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.7e-35
Identity = 124/382 (32.46%), Postives = 193/382 (50.52%), Query Frame = 0

Query: 124 LTDLLLAAAEAVEAQN--QPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKALEY 183
           L  LL+AAA+A    N  + L   ++ +LK+ L+     ++  +LA  FT GL       
Sbjct: 104 LVHLLVAAADASTGANKSRELTRVILARLKD-LVSPGDRTNMERLAAHFTNGLSKLLERD 163

Query: 184 NISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVIDFDI 243
           ++   +    D     + +SAF++LQ +SPY+ F + TA QAILEA + E+ IH++D+DI
Sbjct: 164 SVLCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDI 223

Query: 244 MEGIQWPPLMADLAAKK---EVCLLRLTAIVQDSENERK---IEQTGRRLSEFAKSMNFP 303
            EG+QW  LM  L ++        LR+TA+ + +  ++    +++TGRRL+ FA S+  P
Sbjct: 224 NEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIGQP 283

Query: 304 FMFDQMGIEKE--ENFEQIQVMGQTVIANCSGILH-HILSHRNLSKLETFLSGISKMSPK 363
           F +    ++           V G+ V+ NC  +LH    SH+  S + +FLS    ++PK
Sbjct: 284 FSYQHCKLDTNAFSTSSLKLVRGEAVVINC--MLHLPRFSHQTPSSVISFLSEAKTLNPK 343

Query: 364 CVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFS-GVYENGFKQVMEEFLG 423
            V LV EE   V       F+  F +  H FSA+ DSL    S      GF  V   F+G
Sbjct: 344 LVTLVHEE---VGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPARGF--VERVFIG 403

Query: 424 TRILDSVSQFPC-DKNVRNL--WKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQ 483
             + + +++    D  V +   W    +   G+K +  S  N  QAK L+SLF   F V+
Sbjct: 404 PWVANWLTRITANDAEVESFASWPQWLE-TNGFKPLEVSFTNRCQAKLLLSLFNDGFRVE 463

Query: 484 H-EKCSLSLCWKSRPLCTATIW 490
              +  L L WKSR L +A+ W
Sbjct: 464 ELGQNGLVLGWKSRRLVSASFW 476

BLAST of Spg013457.1 vs. ExPASy Swiss-Prot
Match: G7L166 (GRAS family protein RAM1 OS=Medicago truncatula OX=3880 GN=RAM1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.8e-32
Identity = 122/403 (30.27%), Postives = 195/403 (48.39%), Query Frame = 0

Query: 113 GIQAELMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQ 172
           GI+ E      L  LLLA AEAV      L    + +L N ++  +G  S  ++A  FT+
Sbjct: 286 GIEQEQDSGLQLVHLLLACAEAVAKGEYMLARRYLHQL-NRVVTPLG-DSMQRVASCFTE 345

Query: 173 GLHYK---ALEYNISAHEMADQDQIKSNNSMSA--------------FQMLQQLSPYIKF 232
            L  +    L    S+ +      + S++S S               +Q++ Q  PYIKF
Sbjct: 346 SLSARLAATLTTKSSSTKKLAPSSLSSSSSSSCLSTFPSNPMEVLKIYQIVYQACPYIKF 405

Query: 233 AHFTANQAILEASEGEKLIHVIDFDIMEGIQWPPLMADLAAKK-EVCLLRLTAIVQDSEN 292
           AHFTANQAI EA E E+ +HVID DI++G QWP  M  LAA+      LR+T +    E+
Sbjct: 406 AHFTANQAIFEAFEAEERVHVIDLDILQGYQWPAFMQALAARPGGAPFLRITGVGPCIES 465

Query: 293 ERKIEQTGRRLSEFAKSMNFPFMFDQMGIEKEENFEQI--QVMGQTVIANCSGILHHILS 352
              + +TGR L+E A S+  PF F  +G + E+    +  + +G+ +  N    LH +  
Sbjct: 466 ---VRETGRCLTELAHSLRIPFEFHPVGEQLEDLKPHMFNRRVGEALAVNTVNRLHRVPG 525

Query: 353 HRNLSKLETFLSGISKMSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVR 412
           +     L   LS I   +P  V LVE+E    S   P  F+  F EA H++SA+ DSL  
Sbjct: 526 NH----LGNLLSMIRDQAPNIVTLVEQE---ASHNGPY-FLGRFLEALHYYSAIFDSLDA 585

Query: 413 CFSGVYENGFKQVMEEFLGTRILDSVSQFPCDKNVR-----NLWKNAFDHLKGYKRIPFS 472
            F    E+  +  +E+++    + ++     ++ +        W+   +  KG+K +P S
Sbjct: 586 TFP--VESAPRAKVEQYIFAPEIRNIVACEGEERIERHERLEKWRKIMEG-KGFKGVPLS 645

Query: 473 SFNCSQAKYLISLFRGD-FWVQHEKCSLSLCWKSRPLCTATIW 490
               +Q++ L+ L+  D + +  +K  L L W+ R +  A+ W
Sbjct: 646 PNAVTQSRILLGLYSCDGYRLTEDKGCLLLGWQDRAIIAASAW 672

BLAST of Spg013457.1 vs. ExPASy TrEMBL
Match: A0A6J1HDU3 (nodulation-signaling pathway 2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463069 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 5.1e-226
Identity = 411/498 (82.53%), Postives = 449/498 (90.16%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFF-QQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY++F QQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFSQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP I Y  EDFL TTMDHQ+I  MG+SRSV  +EEEEEENS  +FKGIQAE
Sbjct: 64  -----LDQFDP-INYNFEDFLATTMDHQSIPEMGDSRSVQEDEEEEEENSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFTQGLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLMADLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMADLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVIANCSGILH  LSHRN SKLETFL+G++K+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIANCSGILHS-LSHRNSSKLETFLNGVAKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGVYENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVYENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC+KN  NL + AFD+ KGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  SLSL
Sbjct: 424 SVSQFPCEKNAMNLREKAFDYFKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGSLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. ExPASy TrEMBL
Match: A0A6J1KQT9 (nodulation-signaling pathway 2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111497427 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 9.6e-225
Identity = 411/498 (82.53%), Postives = 447/498 (89.76%), Query Frame = 0

Query: 1   MELEPIEFLTNYT-FFQQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVE 60
           MELEPI+FLTNY+ FFQQSH F ECS  NSCSSLSENSSQNSLQDG FLEYADQNLLQ E
Sbjct: 4   MELEPIDFLTNYSYFFQQSHEFGECSLTNSCSSLSENSSQNSLQDGYFLEYADQNLLQQE 63

Query: 61  MLEEALDQFDPIIYYTPEDFLTTTMDHQNISLMGNSRSV--EEEEEEENSNNLFKGIQAE 120
                LDQFDP   Y  EDFLTTTMDHQ+I  MG+SRSV  E+EEEEENS  +FKGIQAE
Sbjct: 64  -----LDQFDP-NNYNFEDFLTTTMDHQSIPEMGDSRSVQEEDEEEEENSKTMFKGIQAE 123

Query: 121 LMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYK 180
           LMEEESLTDLLLAAA+AV+AQN PLVSNLI+KL NLLLCDMGSSSFNQLAWFFTQGLHYK
Sbjct: 124 LMEEESLTDLLLAAADAVDAQNHPLVSNLIQKLTNLLLCDMGSSSFNQLAWFFTQGLHYK 183

Query: 181 ALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVI 240
            + Y+I+AHEMA+QDQ  + NS+SAF MLQQLSPYIKFAHFTANQAILEASEGEK+IHVI
Sbjct: 184 TVGYDIAAHEMAEQDQTMNKNSLSAFLMLQQLSPYIKFAHFTANQAILEASEGEKMIHVI 243

Query: 241 DFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENE-RKIEQTGRRLSEFAKSMNFPF 300
           DFDIMEGIQWPPLM DLAAKK+VCLLRLTAI Q +ENE R+IE+TGRRLSEFAKS+N PF
Sbjct: 244 DFDIMEGIQWPPLMEDLAAKKQVCLLRLTAIEQGNENEQRRIEETGRRLSEFAKSINLPF 303

Query: 301 MFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVL 360
            FD MGIEK+ENFEQIQV+GQTVI NCSGILH  LSHRN SKLETFL+GISK+SPKCVVL
Sbjct: 304 KFDHMGIEKDENFEQIQVLGQTVIGNCSGILHS-LSHRNSSKLETFLNGISKLSPKCVVL 363

Query: 361 VEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILD 420
           VEEELFKVSKAQPMSFVEFFFEAFHHFS+LSDSL+RCFSGV+ENGFKQVM+EFLGTRIL+
Sbjct: 364 VEEELFKVSKAQPMSFVEFFFEAFHHFSSLSDSLLRCFSGVHENGFKQVMDEFLGTRILE 423

Query: 421 SVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHEKCSLSL 480
           SVSQFPC KN  NL + AFD+LKGYK+IPFSSFNCSQAKYLI+LFRGDFWVQHE  +LSL
Sbjct: 424 SVSQFPCGKNAMNLREKAFDYLKGYKKIPFSSFNCSQAKYLITLFRGDFWVQHENGTLSL 483

Query: 481 CWKSRPLCTATIWVPRVK 495
           CWKSRPLC+ATIWVP  K
Sbjct: 484 CWKSRPLCSATIWVPTAK 494

BLAST of Spg013457.1 vs. ExPASy TrEMBL
Match: A0A0A0KKU8 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G484540 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 385/505 (76.24%), Postives = 423/505 (83.76%), Query Frame = 0

Query: 6   IEFLTNYTFFQQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVEMLEEAL 65
           +EF TNY +F     F    + NSCSSLSENSSQNS Q+GSFLEY DQN L         
Sbjct: 1   MEFFTNYNYFSSPETFFHTHEGNSCSSLSENSSQNSFQEGSFLEYDDQNFL--------- 60

Query: 66  DQFDPIIYYTPEDFLT-TTMDHQNISLMGN-SRSVEE---EEEEENSNN-LFKGIQAELM 125
            QFDP I Y PEDFL  T++D QNI    N S  VEE   EEE+E SNN +FKGIQAELM
Sbjct: 61  -QFDP-IDYNPEDFLAITSIDPQNIQESSNHSGHVEEEDHEEEQEKSNNIIFKGIQAELM 120

Query: 126 EEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKAL 185
           EEESLTDLLLAAAEA+EAQN  LVSNLIEKLKNLLL DMGSSSFNQLAWFFTQGLHYK +
Sbjct: 121 EEESLTDLLLAAAEAIEAQNHVLVSNLIEKLKNLLLYDMGSSSFNQLAWFFTQGLHYKTV 180

Query: 186 EYNI-SAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVID 245
           +YNI +AH    Q QIK+ NSMSAFQMLQQLSPYIKFAHFTANQAILEA+EGEK+IH+ID
Sbjct: 181 DYNILAAHH---QIQIKNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEGEKMIHIID 240

Query: 246 FDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENERK-IEQTGRRLSEFAKSMNFPFM 305
           FDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD+ENERK IEQTGRRLSEFAKS+N PF+
Sbjct: 241 FDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFAKSINLPFI 300

Query: 306 FDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVLV 365
           FDQMGIEK + FE+IQVMG+TVI NCSGI HHILS+ NLSK E FL G+SK+SPKCVVLV
Sbjct: 301 FDQMGIEKADRFEEIQVMGETVIGNCSGIFHHILSYENLSKFEIFLDGVSKLSPKCVVLV 360

Query: 366 EEELFKVSK------AQP-MSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFL 425
           EEELF V+K       QP MSFVEFFFEAFHHFSALSDSL+RCFSGVYENGFKQVM+EFL
Sbjct: 361 EEELFNVTKQLGLGGPQPTMSFVEFFFEAFHHFSALSDSLLRCFSGVYENGFKQVMDEFL 420

Query: 426 GTRILDSVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQHE 485
           GTRILDSV+QFPCDK   ++W + FDHL+GY +IPF+SFNCSQAKYLISLFRGDFWVQHE
Sbjct: 421 GTRILDSVTQFPCDKT--HVWGSGFDHLQGYNKIPFTSFNCSQAKYLISLFRGDFWVQHE 480

Query: 486 KCSLSLCWKSRPLCTATIWVPRVKS 496
           KC+LSLCWKSRPLCTATIWVP V+S
Sbjct: 481 KCNLSLCWKSRPLCTATIWVPSVES 489

BLAST of Spg013457.1 vs. ExPASy TrEMBL
Match: A0A5D3DPX1 (Nodulation-signaling pathway 2 protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00340 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.3e-200
Identity = 382/510 (74.90%), Postives = 423/510 (82.94%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFFQQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVEM 60
           ME E +EF TNY +F     F    + NSCSSLSENSSQNS Q+GSFLEY DQ+ L    
Sbjct: 4   MEFESMEFFTNYNYFSSPEAFFHTHEGNSCSSLSENSSQNSFQEGSFLEYDDQSFL---- 63

Query: 61  LEEALDQFDPIIYYTPEDFL-TTTMDHQ-NISLMGNSRSVE----EEEEEENSNNLFKGI 120
                 QFDP I Y PED L  T++D Q N  L  +S  V+    EEE++E++N +FKGI
Sbjct: 64  ------QFDP-INYNPEDLLAVTSIDPQNNQELSDHSGHVDEEDHEEEQDESNNIIFKGI 123

Query: 121 QAELMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGL 180
           QAELMEEESLTDLLLAAAEA+EAQN  LVSNLIEKLKNLLL DMGSSSFNQLAWFFTQGL
Sbjct: 124 QAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAWFFTQGL 183

Query: 181 HYKALEYNI-SAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKL 240
           HYK ++ NI +AH    Q QI + NSMSAFQMLQQLSPYIKFAHFTANQAILEA+EGE++
Sbjct: 184 HYKTVDNNILAAHH---QIQINNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEGERM 243

Query: 241 IHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENERK-IEQTGRRLSEFAKSM 300
           IH+IDFDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD+ENERK IEQTGRRLSEFAKS+
Sbjct: 244 IHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFAKSV 303

Query: 301 NFPFMFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPK 360
           N PF+FDQ+GIEK + FE+IQVMG+TVIANCSGI HHILS+ NLSK  TFL G+SK+SPK
Sbjct: 304 NLPFIFDQIGIEKADKFEEIQVMGETVIANCSGIFHHILSYENLSKFGTFLDGVSKLSPK 363

Query: 361 CVVLVEEELFKVSK------AQP-MSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQV 420
           CVVLVEEELF V+K       QP MSFVEFFFEAFHHFSALSDSL+RCFSGVYENGFKQV
Sbjct: 364 CVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSGVYENGFKQV 423

Query: 421 MEEFLGTRILDSVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDF 480
           M+EFLGTRILDSV+QFPCDK     W + FDHLKGYK+IPFSSFNCSQAKYLISLFRGDF
Sbjct: 424 MDEFLGTRILDSVTQFPCDK-THVSWGSGFDHLKGYKKIPFSSFNCSQAKYLISLFRGDF 483

Query: 481 WVQHEKCSLSLCWKSRPLCTATIWVPRVKS 496
           WVQHEKCSLSLCWKSRPLCTATIWVP V+S
Sbjct: 484 WVQHEKCSLSLCWKSRPLCTATIWVPCVES 498

BLAST of Spg013457.1 vs. ExPASy TrEMBL
Match: A0A1S3B384 (nodulation-signaling pathway 2 protein-like OS=Cucumis melo OX=3656 GN=LOC103485258 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.3e-200
Identity = 382/510 (74.90%), Postives = 423/510 (82.94%), Query Frame = 0

Query: 1   MELEPIEFLTNYTFFQQSHGFVECSQANSCSSLSENSSQNSLQDGSFLEYADQNLLQVEM 60
           ME E +EF TNY +F     F    + NSCSSLSENSSQNS Q+GSFLEY DQ+ L    
Sbjct: 4   MEFESMEFFTNYNYFSSPEAFFHTHEGNSCSSLSENSSQNSFQEGSFLEYDDQSFL---- 63

Query: 61  LEEALDQFDPIIYYTPEDFL-TTTMDHQ-NISLMGNSRSVE----EEEEEENSNNLFKGI 120
                 QFDP I Y PED L  T++D Q N  L  +S  V+    EEE++E++N +FKGI
Sbjct: 64  ------QFDP-INYNPEDLLAVTSIDPQNNQELSDHSGHVDEEDHEEEQDESNNIIFKGI 123

Query: 121 QAELMEEESLTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGL 180
           QAELMEEESLTDLLLAAAEA+EAQN  LVSNLIEKLKNLLL DMGSSSFNQLAWFFTQGL
Sbjct: 124 QAELMEEESLTDLLLAAAEAIEAQNHLLVSNLIEKLKNLLLYDMGSSSFNQLAWFFTQGL 183

Query: 181 HYKALEYNI-SAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKL 240
           HYK ++ NI +AH    Q QI + NSMSAFQMLQQLSPYIKFAHFTANQAILEA+EGE++
Sbjct: 184 HYKTVDNNILAAHH---QIQINNYNSMSAFQMLQQLSPYIKFAHFTANQAILEAAEGERM 243

Query: 241 IHVIDFDIMEGIQWPPLMADLAAKKEVCLLRLTAIVQDSENERK-IEQTGRRLSEFAKSM 300
           IH+IDFDIMEGIQWPPLMADLAAK+ VC LRLTAIVQD+ENERK IEQTGRRLSEFAKS+
Sbjct: 244 IHIIDFDIMEGIQWPPLMADLAAKEHVCSLRLTAIVQDNENERKIIEQTGRRLSEFAKSV 303

Query: 301 NFPFMFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPK 360
           N PF+FDQ+GIEK + FE+IQVMG+TVIANCSGI HHILS+ NLSK  TFL G+SK+SPK
Sbjct: 304 NLPFIFDQIGIEKADKFEEIQVMGETVIANCSGIFHHILSYENLSKFGTFLDGVSKLSPK 363

Query: 361 CVVLVEEELFKVSK------AQP-MSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQV 420
           CVVLVEEELF V+K       QP MSFVEFFFEAFHHFSALSDSL+RCFSGVYENGFKQV
Sbjct: 364 CVVLVEEELFNVTKQLGLGGPQPIMSFVEFFFEAFHHFSALSDSLLRCFSGVYENGFKQV 423

Query: 421 MEEFLGTRILDSVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDF 480
           M+EFLGTRILDSV+QFPCDK     W + FDHLKGYK+IPFSSFNCSQAKYLISLFRGDF
Sbjct: 424 MDEFLGTRILDSVTQFPCDK-THVSWGSGFDHLKGYKKIPFSSFNCSQAKYLISLFRGDF 483

Query: 481 WVQHEKCSLSLCWKSRPLCTATIWVPRVKS 496
           WVQHEKCSLSLCWKSRPLCTATIWVP V+S
Sbjct: 484 WVQHEKCSLSLCWKSRPLCTATIWVPCVES 498

BLAST of Spg013457.1 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 150.6 bits (379), Expect = 3.3e-36
Identity = 124/382 (32.46%), Postives = 193/382 (50.52%), Query Frame = 0

Query: 124 LTDLLLAAAEAVEAQN--QPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKALEY 183
           L  LL+AAA+A    N  + L   ++ +LK+ L+     ++  +LA  FT GL       
Sbjct: 104 LVHLLVAAADASTGANKSRELTRVILARLKD-LVSPGDRTNMERLAAHFTNGLSKLLERD 163

Query: 184 NISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVIDFDI 243
           ++   +    D     + +SAF++LQ +SPY+ F + TA QAILEA + E+ IH++D+DI
Sbjct: 164 SVLCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIHIVDYDI 223

Query: 244 MEGIQWPPLMADLAAKK---EVCLLRLTAIVQDSENERK---IEQTGRRLSEFAKSMNFP 303
            EG+QW  LM  L ++        LR+TA+ + +  ++    +++TGRRL+ FA S+  P
Sbjct: 224 NEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGRRLTAFADSIGQP 283

Query: 304 FMFDQMGIEKE--ENFEQIQVMGQTVIANCSGILH-HILSHRNLSKLETFLSGISKMSPK 363
           F +    ++           V G+ V+ NC  +LH    SH+  S + +FLS    ++PK
Sbjct: 284 FSYQHCKLDTNAFSTSSLKLVRGEAVVINC--MLHLPRFSHQTPSSVISFLSEAKTLNPK 343

Query: 364 CVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFS-GVYENGFKQVMEEFLG 423
            V LV EE   V       F+  F +  H FSA+ DSL    S      GF  V   F+G
Sbjct: 344 LVTLVHEE---VGLMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPARGF--VERVFIG 403

Query: 424 TRILDSVSQFPC-DKNVRNL--WKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRGDFWVQ 483
             + + +++    D  V +   W    +   G+K +  S  N  QAK L+SLF   F V+
Sbjct: 404 PWVANWLTRITANDAEVESFASWPQWLE-TNGFKPLEVSFTNRCQAKLLLSLFNDGFRVE 463

Query: 484 H-EKCSLSLCWKSRPLCTATIW 490
              +  L L WKSR L +A+ W
Sbjct: 464 ELGQNGLVLGWKSRRLVSASFW 476

BLAST of Spg013457.1 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 136.3 bits (342), Expect = 6.5e-32
Identity = 113/377 (29.97%), Postives = 183/377 (48.54%), Query Frame = 0

Query: 124 LTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKALEYNI 183
           L  LLL  AE V   +    S L+ ++    +C    SS  ++  +F Q L  + +   +
Sbjct: 40  LLSLLLQCAEYVATDHLREASTLLSEISE--ICSPFGSSPERVVAYFAQALQTRVISSYL 99

Query: 184 SAHEMADQDQ----IKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIHVIDF 243
           S       ++    ++S    SA Q    +SP IKF+HFTANQAI +A +GE  +H+ID 
Sbjct: 100 SGACSPLSEKPLTVVQSQKIFSALQTYNSVSPLIKFSHFTANQAIFQALDGEDSVHIIDL 159

Query: 244 DIMEGIQWPPLMADLAAK-KEVCLLRLTAIVQDSENERKIEQTGRRLSEFAKSMNFPFMF 303
           D+M+G+QWP L   LA++ +++  +R+T     S+    +  TGRRL++FA S+N PF F
Sbjct: 160 DVMQGLQWPALFHILASRPRKLRSIRITGFGSSSD---LLASTGRRLADFASSLNLPFEF 219

Query: 304 DQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHR----NLSKLETFLSGISKMSPKCV 363
             +    E     +    Q        ++ H + HR      + LET L  + ++ P  +
Sbjct: 220 HPI----EGIIGNLIDPSQLATRQGEAVVVHWMQHRLYDVTGNNLET-LEILRRLKPNLI 279

Query: 364 VLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEF-LGTR 423
            +VE+EL   S     SF+  F EA H++SAL D+L        E+G +  +E+  LGT 
Sbjct: 280 TVVEQEL---SYDDGGSFLGRFVEALHYYSALFDALGDGLG--EESGERFTVEQIVLGTE 339

Query: 424 ILDSVSQFPCDKNVRNLWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFR-GDFWVQHEKC 483
           I + V+     +  R  WK     + G++ +       +QA  L+ +     + +  E  
Sbjct: 340 IRNIVAH-GGGRRKRMKWKEELSRV-GFRPVSLRGNPATQAGLLLGMLPWNGYTLVEENG 399

Query: 484 SLSLCWKSRPLCTATIW 490
           +L L WK   L TA+ W
Sbjct: 400 TLRLGWKDLSLLTASAW 399

BLAST of Spg013457.1 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 136.0 bits (341), Expect = 8.5e-32
Identity = 120/390 (30.77%), Postives = 192/390 (49.23%), Query Frame = 0

Query: 114 IQAELMEEES--LTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFT 173
           I+ +  +EE   L  LLL  AEAV A N    + L+ ++    L     +S  ++A +F+
Sbjct: 278 IKRQKQDEEGLHLLTLLLQCAEAVSADNLEEANKLLLEISQ--LSTPYGTSAQRVAAYFS 337

Query: 174 QGLHYKALEYNISAHEMAD---QDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEAS 233
           + +  + L   +  +         Q  S   +SAFQ+   +SP +KF+HFTANQAI EA 
Sbjct: 338 EAMSARLLNSCLGIYAALPSRWMPQTHSLKMVSAFQVFNGISPLVKFSHFTANQAIQEAF 397

Query: 234 EGEKLIHVIDFDIMEGIQWPPLMADLAAKK-EVCLLRLTAIVQDSENERKIEQTGRRLSE 293
           E E  +H+ID DIM+G+QWP L   LA++      +RLT +    E    ++ TG+RLS+
Sbjct: 398 EKEDSVHIIDLDIMQGLQWPGLFHILASRPGGPPHVRLTGLGTSME---ALQATGKRLSD 457

Query: 294 FAKSMNFPFMFDQMGIEKEENFEQIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGIS 353
           FA  +  PF F  +  EK  N +  ++  +   A     L H L     S   T L  + 
Sbjct: 458 FADKLGLPFEFCPLA-EKVGNLDTERLNVRKREAVAVHWLQHSLYDVTGSDAHT-LWLLQ 517

Query: 354 KMSPKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVME 413
           +++PK V +VE++L     +   SF+  F EA H++SAL DSL   +    E+  + V+E
Sbjct: 518 RLAPKVVTVVEQDL-----SHAGSFLGRFVEAIHYYSALFDSLGASYG--EESEERHVVE 577

Query: 414 EFLGTRILDSVSQFPCDKNVRNL----WKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRG 473
           + L ++ + +V           +    W+       G+K I  +    +QA  L+ +F  
Sbjct: 578 QQLLSKEIRNVLAVGGPSRSGEVKFESWREKMQQC-GFKGISLAGNAATQATLLLGMFPS 637

Query: 474 D-FWVQHEKCSLSLCWKSRPLCTATIWVPR 493
           D + +  +  +L L WK   L TA+ W PR
Sbjct: 638 DGYTLVDDNGTLKLGWKDLSLLTASAWTPR 652

BLAST of Spg013457.1 vs. TAIR 10
Match: AT5G17490.1 (RGA-like protein 3 )

HSP 1 Score: 132.5 bits (332), Expect = 9.3e-31
Identity = 115/386 (29.79%), Postives = 190/386 (49.22%), Query Frame = 0

Query: 118 LMEEES--LTDLLLAAAEAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLH 177
           L+EE    L   L+A AEAV+ +N  L   L++++   LL    + +  ++A +F + L 
Sbjct: 149 LIEETGVRLVQALVACAEAVQLENLSLADALVKRVG--LLAASQAGAMGKVATYFAEALA 208

Query: 178 YKALEYNISAHEMADQDQIKSNNSMSAFQMLQQLSPYIKFAHFTANQAILEASEGEKLIH 237
            +   Y I     A     +    M+ +       PY+KFAHFTANQAILEA    +++H
Sbjct: 209 RRI--YRIHPSAAAIDPSFEEILQMNFYDS----CPYLKFAHFTANQAILEAVTTSRVVH 268

Query: 238 VIDFDIMEGIQWPPLMADLAAKK-EVCLLRLTAIVQDSENERKIEQTGRRLSEFAKSMNF 297
           VID  + +G+QWP LM  LA +       RLT +   S N   I++ G +L++ A+++  
Sbjct: 269 VIDLGLNQGMQWPALMQALALRPGGPPSFRLTGVGNPS-NREGIQELGWKLAQLAQAIGV 328

Query: 298 PFMFDQMGIEKEENFE----QIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMS 357
            F F+ +  E+  + E    + +   +T++ N    LH +LS      +E  L+ +  + 
Sbjct: 329 EFKFNGLTTERLSDLEPDMFETRTESETLVVNSVFELHPVLSQP--GSIEKLLATVKAVK 388

Query: 358 PKCVVLVEEELFKVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEE-F 417
           P  V +VE+E    +      F++ F EA H++S+L DSL     GV      +VM E +
Sbjct: 389 PGLVTVVEQE----ANHNGDVFLDRFNEALHYYSSLFDSLE---DGVVIPSQDRVMSEVY 448

Query: 418 LGTRILDSVSQFPCDKNVRN----LWKNAFDHLKGYKRIPFSSFNCSQAKYLISLFRG-- 477
           LG +IL+ V+    D+  R+     W+       G+  +   S    QA  L++L  G  
Sbjct: 449 LGRQILNLVATEGSDRIERHETLAQWRKRMGS-AGFDPVNLGSDAFKQASLLLALSGGGD 508

Query: 478 DFWVQHEKCSLSLCWKSRPLCTATIW 490
            + V+    SL L W+++PL  A+ W
Sbjct: 509 GYRVEENDGSLMLAWQTKPLIAASAW 515

BLAST of Spg013457.1 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 129.4 bits (324), Expect = 7.9e-30
Identity = 127/433 (29.33%), Postives = 209/433 (48.27%), Query Frame = 0

Query: 90  SLMGNSRSVEEEE---EEENSNNLFKGIQAELMEEES--------------LTDLLLAAA 149
           ++ G S   +EEE   EE +S  +  G   E  +E +              L   L+A A
Sbjct: 129 AIPGLSAFPKEEEVFDEEASSKRIRLGSWCESSDESTRSVVLVDSQETGVRLVHALVACA 188

Query: 150 EAVEAQNQPLVSNLIEKLKNLLLCDMGSSSFNQLAWFFTQGLHYKALEYNISAHEMADQD 209
           EA+  +N  L   L++++  L     G  +  ++A +F Q     AL   I     A+ D
Sbjct: 189 EAIHQENLNLADALVKRVGTLAGSQAG--AMGKVATYFAQ-----ALARRIYRDYTAETD 248

Query: 210 QIKSNNSMSAFQMLQ----QLSPYIKFAHFTANQAILEASEGEKLIHVIDFDIMEGIQWP 269
              + N  S  ++L+    +  PY+KFAHFTANQAILEA    + +HVID  + +G+QWP
Sbjct: 249 VCAAVNP-SFEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQGMQWP 308

Query: 270 PLMADLAAKK-EVCLLRLTAI-VQDSENERKIEQTGRRLSEFAKSMNFPFMFDQMGIEKE 329
            LM  LA +       RLT I    +EN   ++Q G +L++FA++M   F F  +  E  
Sbjct: 309 ALMQALALRPGGPPSFRLTGIGPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLAAESL 368

Query: 330 ENFE----QIQVMGQTVIANCSGILHHILSHRNLSKLETFLSGISKMSPKCVVLVEEELF 389
            + E    + +   +T++ N    LH +L+      +E  L+ +  + P  V +VE+E  
Sbjct: 369 SDLEPEMFETRPESETLVVNSVFELHRLLARS--GSIEKLLNTVKAIKPSIVTVVEQE-- 428

Query: 390 KVSKAQPMSFVEFFFEAFHHFSALSDSLVRCFSGVYENGFKQVMEEFLGTRILDSVSQFP 449
             +    + F++ F EA H++S+L DSL   +S   ++  + + E +LG +IL+ V+   
Sbjct: 429 --ANHNGIVFLDRFNEALHYYSSLFDSLEDSYSLPSQD--RVMSEVYLGRQILNVVAAEG 488

Query: 450 CDKNVRN----LWKNAFDHLKGYKRIPFSSFNCSQAKYLISLF-RGD-FWVQHEKCSLSL 490
            D+  R+     W+       G+  I   S    QA  L+SL+  GD + V+     L +
Sbjct: 489 SDRVERHETAAQWRIRMKS-AGFDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMI 544