Spg013274.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg013274.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioninteractor of constitutive active ROPs 2, chloroplastic
Locationscaffold1: 13466296 .. 13470563 (+)
Sequence length2316
RNA-Seq ExpressionSpg013274.1
SyntenySpg013274.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAACAAGCTCAGATTTCAGAGAGTTGCTGTTTTCACATGGCATGAAGTGTTTGATAAGAATCGTGAAACAAAATTTATCTCCTTGTGTTCATATGGGCAGCATAAAGGAAAATTAGGCATTTCCATGCATAGAACACAGCTCTGTAGTGGTCCTCCTCTTCTGTTCATGGAGAAGCCCATCTGTGAGATGAGACCCCAACAATCACGGGTCTTTTTCATCACATGGATGCCCACATGTTAGTCAAGGCGACGACGCAATTGCTTAGAGAAAAAAAATTGAGCGGTGACCCATATTCAAACCTCTCACACTGTTTCCAAATTTCAATCGCTAAAGTAAAATCCAAATCCAAAGTTGTTGAAATCTGATGGCTGAGTTCTACCTTCCGAGTCAAAAACAGAGTTGGGGGAAGGGGAAAAACGAGTTCAGAGTAAGTGATCCTGCCGAATCACGTTACTTTTTAAGCTTCGTCTGTGACTTCTTGTCTTGCGGCACATAAAAACAAAACTTTTCTCTGAGGACAAAGTTCGAACACCTGGTGGGCGTAGCTCATTTCGGCGAAATTTTCTTCCCCCGTTGGCCGTTTTGGCAGTAGTTGAAAACCCAATGAGGAAATTTGGAGCTGACGTTTCTGCTTTGTGGGTAAGCAGTAAGATTTGACAGTGGCAGTGAGATCCCTTTGTGATTCCTCTCACAAGCTTTAACGCGGTAGGAACGAAGGAAAGTCCTTGGCCACCGCCGAGACTGTAGGCTTATATACTACTGGCTACAATGAGCTAGAGAGAGAGAGAGAATTTGTTTGTTTTTCAGAGCATAATGGCAAGCCAGGTTGTTTTTCAGAGCAGTTTGCTGTGAAGCTACTACTAGCTTTTTGGTAAGATCAAAGAACCTGCAAGGAAATTGTTAAGAATGCTTGGTTTTTGTGCTAGTTTTCCTATCTTGAAAGTTCCTAGTCTGTTTGAGTTGAAAGTTATGGTTCCTTGATTTGTTTGATTGAGGCAAATTTTGCTTGTGATTCCTTATTCTGGGCTGAATTTTAATTTAGGGGATCATCTGGGGTTTTTGGTTTTCTTTTTGTTTCCTGTTCTTCATATGATTGTTAGCCGCAGTTCCACGACAACAATATCTGATCTACTGGGTTTCAGTACAAATGCTTCATGATATTCTTTACTGTTTCTCAAATAAACAAACAAACAGGTTGGCCCAGGGAGAGGGAGAGAGAGTTGAAGATTATGCAACTTTACATGTCGCCATGATTTGTTGGGGCCAACAGAGCACTGTGAAAGTAATGAAGTGAAGAAAGTTGAGAAAAGAGATTCGAGAGAAAGCATTTCCATATACCTTTTCTTTTGGTTTACGTATTTTTTCTATAAATCTTACCAAAGTGGCTTTGGTTGGTAGAAGTGGGGGTGGTTTCTCTGGAGTTTCTTTGAAGGGAGTGCTTGTTATTTTGGTTGTTACATTTTTCTGTTGATTCTGATTAAGGCTACCATCTGAATTTTTGTAAAGTATTTTACTTGTTATTGCATTCAGCTTTTCTTGTTATGTTTGATTTTTAATGAAATAATTCTCTGAACTGAAGCAGCTCTTGTGGGTCAGAGAGCTTTGGAGGAGAATACCAACTATTCATTCATCCTGCTTTTATTCTTCAGCTGGAGCTGCAAAATATGCAGACTTCAAAGCCTAAGTAAGATTGCCATTCTTCCTTGTTCTTTTTATTTCTTTAACTAAATTGATATTGGCATCATTTTCATTTTCCTTCTTGATTCTAGAATACCCCCCCCCCCCCCCTCTCCCACTTTTTGGGTCATCCATGGCTGGGGTGCTGATTGTCTTTCTGCTGTGATTGCAGAAGTTTGGAAGCACCACTGAGAAAACCTCGACCCGTTCGACAACTTAAAGCTCCGGGTTCAGATTCTGTTTCGGCTTCCTCTGCTCCACTTCCTCCAAGTAAAATGACAAAGGAAAGAAATCCTAAGACTGTTGTTACAAAATCAGTTCAAAGTTCAGTACTTGAGGTATTTCTCCTCAACAATGGTCCTGCTTGTTTAGGAAGCTGTTGTGAATTCAGTTGTATATATTTTTAGAATAACATTTCAGTGAAAACGTGTTCCAATTTCTATTCTTTGGAAGTTGTTTTATCTCATTTTCTATTAGTTTGATTTCTAGCCAATTAAAGTTTTTAATCCCTATAATTCCGACTCGAACTCCCAATTTTTCTTGGAAAAACTGTCTATAAACTTATTTGCATAGGGTATGTATCTTCAAGTCCTTCGCAGATGAGAATTGCTCACTTGCATCATGTTCTCTGTCTGCAGCTTTGAGAACAATTTCCTTTTACATGATTTACATTGTTGGTGTATCTTTGAACTCCAGAAAAAACGCCCAAATCGAGTTTCTACAATGGAATCACAGATTGTTCAACTTCAAGATGAACTTAAAAAGGCAAAGGATCAACTGAACTCCTCTGAATCAGGGAAGAAGAGAGCAAAAGAGGAAGCTGAAGAGGTTAAGCAGCAGCTTGAAGCCATGTCCTTGGAGCTTGAGGAATCCAGACAGCAGTTGCTAGAGCTTTCTGCTTCGGAAGATGAGCGGATTCAAGAGCTACGTAAAATTTCACTGGATCGAGATAGAGCATGGCAATCTGAACTAGAAGCCCTTCAAAAGCAGCATTTAATGGATTCTGCTGCTTTGGCTGCTGCCATGAATGAAAATCAGAAGCTCAAGCTTCAGCTGGAAAGGATAGCTAGTTCGGAGGCGAACCAGAGTCGACATGCCGAGTCTGCACATGCTGAGATTCAGGGCCTCAGAATAGAACTCAAAGAAACACTTTCACTGGTGGAGGAACTTAAATCCAAGCTTTCTGATTATGAAGATTCTGAAGCTCAGGCTCTTGACGATATGAAGAAAACACAAATGGAACTGGAAACCGCAAATGCAACCATTGAAATGCTTCGATCAGAGGGATCCAAAGCCATGAAAGCATTCAACTCCATATCCTTGGAGTTGGAGCAATACAAAGAAAAAGTTATATCATTGGAGGCTCTTGTTAGCAAATACCAGGATGATTTGGCTGAAATTGACAAGAAAAATTTGGAAGATCACTCTGAAAGTAAGAACAATGACAAGGATAATGAAGAAAATGAACATATTAATCAGCTTAAAGCTGAGCTTAATTGTGTTAGATCTGAAATGGGTCAATTGAGACTGGCATTAGACGCTGCTGATAGAAGGTACCAGGACGAATATCTTCGAAGCACCTTACAGATCAGAAGTTCTTATGAAGAAGTGGAATCTGTAAAATCAGAGTCGCGTCGAAAAGAGGCTGCATTTGAAGCTGAACTCACTCAAGCCAAGGCACAAATCGAGCAACTGAGGACGCGTCTCGAGGATAAGGAAACTCAACTTCTGAGCATTGCCAAGGACAACGAAACTTTAAACTTGAATCAGAAAAGCAAAGAAAGTGAAACCGAATCTGATCTTGCAGAACAGCTGAAGAAGTTTGAGGCTGATATTGAGGAGTTGAAAGCAAGCCTGCTGGATAAAGAGACAGAGTTGCAAGGAATCATTGAAGAAAACGACATGCTCCGGGTCGACATTCAGAAAATGGAAACAGAAAGGAAGATAGGGAACGGCGAAACAACTGATCTGGAAGAACCAGCAAAATCTGCAAATGAAGAGGCTTTGGACAAGCTTGGGAGTGTAAACGAAAACTCGGAAATGGAGGCAGAATTGAGGAGGTTAAGGGTGCAGTTAGACCAATGGAGGAAAGCAGCTGAGGCAGCTGCAGCAATGCTCTCACCAGGGAAAGATGGAAAACTTGTAGAGATTTCAGGTCCCATTGACAGCAACTATCCTCTGGGCTCGTTCTACTCCGAAGACCTCGACGATGACTCGCCGAAGAAGAAGAATATCAACATGCTGAAAAAGATTGGCGTTTTGTGGAAAAAGAGTCAAAAGTAGATATAGAATTGCAGCAGCATTTTCCCTGAAGTTTAAGCAAATTGGGTTGGCTTCTTCTCTTTTATAATTTTGGCTTGAAATCATAGGCAAACAAATATTTTATTTGGAACTGTATAGTATAGTTGCTCAAACTGGTAAGTTTCAATTTGTTACAAATGAATTCATGATGCTCACTTCTGCAAAGCACAATAAGTTTGTCTTTTTTTCATTTGCAGGTGAAAGATATGGAAAAATAATGTATGAATTTCAAGGATTTTTTTAGTCATTTCAAAGGACATATATCTTCATATATACATCAGGGC

mRNA sequence

ATGATGAACAAGCTCAGATTTCAGAGAGTTGCTGTTTTCACATGGCATGAAGTGTTTGATAAGAATCGTGAAACAAAATTTATCTCCTTGTGTTCATATGGGCAGCATAAAGGAAAATTAGGCATTTCCATGCATAGAACACAGCTCTGTAGTGGTCCTCCTCTTCTGTTCATGGAGAAGCCCATCTTTCGAACACCTGGTGGGCGTAGCTCATTTCGGCGAAATTTTCTTCCCCCGTTGGCCGTTTTGGCAGTAGTTGAAAACCCAATGAGGAAATTTGGAGCTGACGTTTCTGCTTTGAACGAAGGAAAGTCCTTGGCCACCGCCGAGACTGTAGGCTTATATACTACTGGCTACAATGAGCTAGAGAGAGAGAGAGAATTTGTTTGTTTTTCAGAGCATAATGGCAAGCCAGGTTGTTTTTCAGAGCAGTTTGCTGTGAAGCTACTACTAGCTTTTTGCTCTTGTGGGTCAGAGAGCTTTGGAGGAGAATACCAACTATTCATTCATCCTGCTTTTATTCTTCAGCTGGAGCTGCAAAATATGCAGACTTCAAAGCCTAAAAGTTTGGAAGCACCACTGAGAAAACCTCGACCCGTTCGACAACTTAAAGCTCCGGGTTCAGATTCTGTTTCGGCTTCCTCTGCTCCACTTCCTCCAAGTAAAATGACAAAGGAAAGAAATCCTAAGACTGTTGTTACAAAATCAGTTCAAAGTTCAGTACTTGAGAAAAAACGCCCAAATCGAGTTTCTACAATGGAATCACAGATTGTTCAACTTCAAGATGAACTTAAAAAGGCAAAGGATCAACTGAACTCCTCTGAATCAGGGAAGAAGAGAGCAAAAGAGGAAGCTGAAGAGGTTAAGCAGCAGCTTGAAGCCATGTCCTTGGAGCTTGAGGAATCCAGACAGCAGTTGCTAGAGCTTTCTGCTTCGGAAGATGAGCGGATTCAAGAGCTACGTAAAATTTCACTGGATCGAGATAGAGCATGGCAATCTGAACTAGAAGCCCTTCAAAAGCAGCATTTAATGGATTCTGCTGCTTTGGCTGCTGCCATGAATGAAAATCAGAAGCTCAAGCTTCAGCTGGAAAGGATAGCTAGTTCGGAGGCGAACCAGAGTCGACATGCCGAGTCTGCACATGCTGAGATTCAGGGCCTCAGAATAGAACTCAAAGAAACACTTTCACTGGTGGAGGAACTTAAATCCAAGCTTTCTGATTATGAAGATTCTGAAGCTCAGGCTCTTGACGATATGAAGAAAACACAAATGGAACTGGAAACCGCAAATGCAACCATTGAAATGCTTCGATCAGAGGGATCCAAAGCCATGAAAGCATTCAACTCCATATCCTTGGAGTTGGAGCAATACAAAGAAAAAGTTATATCATTGGAGGCTCTTGTTAGCAAATACCAGGATGATTTGGCTGAAATTGACAAGAAAAATTTGGAAGATCACTCTGAAAGTAAGAACAATGACAAGGATAATGAAGAAAATGAACATATTAATCAGCTTAAAGCTGAGCTTAATTGTGTTAGATCTGAAATGGGTCAATTGAGACTGGCATTAGACGCTGCTGATAGAAGGTACCAGGACGAATATCTTCGAAGCACCTTACAGATCAGAAGTTCTTATGAAGAAGTGGAATCTGTAAAATCAGAGTCGCGTCGAAAAGAGGCTGCATTTGAAGCTGAACTCACTCAAGCCAAGGCACAAATCGAGCAACTGAGGACGCGTCTCGAGGATAAGGAAACTCAACTTCTGAGCATTGCCAAGGACAACGAAACTTTAAACTTGAATCAGAAAAGCAAAGAAAGTGAAACCGAATCTGATCTTGCAGAACAGCTGAAGAAGTTTGAGGCTGATATTGAGGAGTTGAAAGCAAGCCTGCTGGATAAAGAGACAGAGTTGCAAGGAATCATTGAAGAAAACGACATGCTCCGGGTCGACATTCAGAAAATGGAAACAGAAAGGAAGATAGGGAACGGCGAAACAACTGATCTGGAAGAACCAGCAAAATCTGCAAATGAAGAGGCTTTGGACAAGCTTGGGAGTGTAAACGAAAACTCGGAAATGGAGGCAGAATTGAGGAGGTTAAGGGTGCAGTTAGACCAATGGAGGAAAGCAGCTGAGGCAGCTGCAGCAATGCTCTCACCAGGGAAAGATGGAAAACTTGTAGAGATTTCAGGTCCCATTGACAGCAACTATCCTCTGGGCTCGTTCTACTCCGAAGACCTCGACGATGACTCGCCGAAGAAGAAGAATATCAACATGCTGAAAAAGATTGGCGTTTTGTGGAAAAAGAGTCAAAAGTAG

Coding sequence (CDS)

ATGATGAACAAGCTCAGATTTCAGAGAGTTGCTGTTTTCACATGGCATGAAGTGTTTGATAAGAATCGTGAAACAAAATTTATCTCCTTGTGTTCATATGGGCAGCATAAAGGAAAATTAGGCATTTCCATGCATAGAACACAGCTCTGTAGTGGTCCTCCTCTTCTGTTCATGGAGAAGCCCATCTTTCGAACACCTGGTGGGCGTAGCTCATTTCGGCGAAATTTTCTTCCCCCGTTGGCCGTTTTGGCAGTAGTTGAAAACCCAATGAGGAAATTTGGAGCTGACGTTTCTGCTTTGAACGAAGGAAAGTCCTTGGCCACCGCCGAGACTGTAGGCTTATATACTACTGGCTACAATGAGCTAGAGAGAGAGAGAGAATTTGTTTGTTTTTCAGAGCATAATGGCAAGCCAGGTTGTTTTTCAGAGCAGTTTGCTGTGAAGCTACTACTAGCTTTTTGCTCTTGTGGGTCAGAGAGCTTTGGAGGAGAATACCAACTATTCATTCATCCTGCTTTTATTCTTCAGCTGGAGCTGCAAAATATGCAGACTTCAAAGCCTAAAAGTTTGGAAGCACCACTGAGAAAACCTCGACCCGTTCGACAACTTAAAGCTCCGGGTTCAGATTCTGTTTCGGCTTCCTCTGCTCCACTTCCTCCAAGTAAAATGACAAAGGAAAGAAATCCTAAGACTGTTGTTACAAAATCAGTTCAAAGTTCAGTACTTGAGAAAAAACGCCCAAATCGAGTTTCTACAATGGAATCACAGATTGTTCAACTTCAAGATGAACTTAAAAAGGCAAAGGATCAACTGAACTCCTCTGAATCAGGGAAGAAGAGAGCAAAAGAGGAAGCTGAAGAGGTTAAGCAGCAGCTTGAAGCCATGTCCTTGGAGCTTGAGGAATCCAGACAGCAGTTGCTAGAGCTTTCTGCTTCGGAAGATGAGCGGATTCAAGAGCTACGTAAAATTTCACTGGATCGAGATAGAGCATGGCAATCTGAACTAGAAGCCCTTCAAAAGCAGCATTTAATGGATTCTGCTGCTTTGGCTGCTGCCATGAATGAAAATCAGAAGCTCAAGCTTCAGCTGGAAAGGATAGCTAGTTCGGAGGCGAACCAGAGTCGACATGCCGAGTCTGCACATGCTGAGATTCAGGGCCTCAGAATAGAACTCAAAGAAACACTTTCACTGGTGGAGGAACTTAAATCCAAGCTTTCTGATTATGAAGATTCTGAAGCTCAGGCTCTTGACGATATGAAGAAAACACAAATGGAACTGGAAACCGCAAATGCAACCATTGAAATGCTTCGATCAGAGGGATCCAAAGCCATGAAAGCATTCAACTCCATATCCTTGGAGTTGGAGCAATACAAAGAAAAAGTTATATCATTGGAGGCTCTTGTTAGCAAATACCAGGATGATTTGGCTGAAATTGACAAGAAAAATTTGGAAGATCACTCTGAAAGTAAGAACAATGACAAGGATAATGAAGAAAATGAACATATTAATCAGCTTAAAGCTGAGCTTAATTGTGTTAGATCTGAAATGGGTCAATTGAGACTGGCATTAGACGCTGCTGATAGAAGGTACCAGGACGAATATCTTCGAAGCACCTTACAGATCAGAAGTTCTTATGAAGAAGTGGAATCTGTAAAATCAGAGTCGCGTCGAAAAGAGGCTGCATTTGAAGCTGAACTCACTCAAGCCAAGGCACAAATCGAGCAACTGAGGACGCGTCTCGAGGATAAGGAAACTCAACTTCTGAGCATTGCCAAGGACAACGAAACTTTAAACTTGAATCAGAAAAGCAAAGAAAGTGAAACCGAATCTGATCTTGCAGAACAGCTGAAGAAGTTTGAGGCTGATATTGAGGAGTTGAAAGCAAGCCTGCTGGATAAAGAGACAGAGTTGCAAGGAATCATTGAAGAAAACGACATGCTCCGGGTCGACATTCAGAAAATGGAAACAGAAAGGAAGATAGGGAACGGCGAAACAACTGATCTGGAAGAACCAGCAAAATCTGCAAATGAAGAGGCTTTGGACAAGCTTGGGAGTGTAAACGAAAACTCGGAAATGGAGGCAGAATTGAGGAGGTTAAGGGTGCAGTTAGACCAATGGAGGAAAGCAGCTGAGGCAGCTGCAGCAATGCTCTCACCAGGGAAAGATGGAAAACTTGTAGAGATTTCAGGTCCCATTGACAGCAACTATCCTCTGGGCTCGTTCTACTCCGAAGACCTCGACGATGACTCGCCGAAGAAGAAGAATATCAACATGCTGAAAAAGATTGGCGTTTTGTGGAAAAAGAGTCAAAAGTAG

Protein sequence

MMNKLRFQRVAVFTWHEVFDKNRETKFISLCSYGQHKGKLGISMHRTQLCSGPPLLFMEKPIFRTPGGRSSFRRNFLPPLAVLAVVENPMRKFGADVSALNEGKSLATAETVGLYTTGYNELEREREFVCFSEHNGKPGCFSEQFAVKLLLAFCSCGSESFGGEYQLFIHPAFILQLELQNMQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIGNGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Homology
BLAST of Spg013274.1 vs. NCBI nr
Match: KAA0047284.1 (interactor of constitutive active ROPs 2 [Cucumis melo var. makuwa])

HSP 1 Score: 952.2 bits (2460), Expect = 2.7e-273
Identity = 541/632 (85.60%), Postives = 582/632 (92.09%), Query Frame = 0

Query: 140 CFSEQFAVKLLLAFCSCGSESFGGEYQLFIHPAFILQLELQNMQTSKPKSLEAPLRKPRP 199
           C+ +Q  VK          +SF GEYQLFI PAFILQ ELQNMQT KP+SLEAPLRKPRP
Sbjct: 10  CWGQQSTVK---------EKSFEGEYQLFIQPAFILQRELQNMQTPKPRSLEAPLRKPRP 69

Query: 200 VRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTMESQIVQ 259
           +RQLK+PG+DSVSAS +PLPPSKMTKER+PKT V KSVQSSVLEKKRPNRVST+ESQI Q
Sbjct: 70  IRQLKSPGTDSVSASVSPLPPSKMTKERSPKT-VAKSVQSSVLEKKRPNRVSTVESQIAQ 129

Query: 260 LQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEESRQQLLELSASEDERIQE 319
           LQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+SRQQLLELSASEDERIQE
Sbjct: 130 LQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLLELSASEDERIQE 189

Query: 320 LRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKLQLERIASSEANQSRHAES 379
           LRKIS DRDRAWQSELEALQKQHLMDSAAL AAM+ENQKLKLQLERIA SEANQSRHAES
Sbjct: 190 LRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKLQLERIAGSEANQSRHAES 249

Query: 380 AHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKKTQMELETANATIEMLRSE 439
           AHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KKTQMELETAN TIE L+SE
Sbjct: 250 AHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKTQMELETANKTIERLQSE 309

Query: 440 GSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKKNLEDHSESKNNDKDNEEN 499
           G+ AMKAF+SISLELEQ KEKV +LEALVSKYQDDLAEIDKKNLEDHSE+KNNDKD+EEN
Sbjct: 310 GTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKKNLEDHSENKNNDKDDEEN 369

Query: 500 EHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQIRSSYEEVESVKSESRRKE 559
           E+INQL  ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QIRSSYEEVES+KSESRRKE
Sbjct: 370 EYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKE 429

Query: 560 AAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQKSKESETESDLAEQLKKF 619
           AAFEAEL QAK QI+QLRT LEDKE QL+S+AK+ ET +LNQKSKESE E+D++EQLKKF
Sbjct: 430 AAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQKSKESEIETDISEQLKKF 489

Query: 620 EADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIGNGETTDLEEPAKSANEEA 679
           E+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI +GETTDLEEP KSANEE 
Sbjct: 490 ESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPTKSANEET 549

Query: 680 LDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVEISGPIDSNYPLG 739
           L+KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLV+I+G IDSNYPL 
Sbjct: 550 LNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLS 609

Query: 740 SFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 610 SYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 631

BLAST of Spg013274.1 vs. NCBI nr
Match: XP_038882490.1 (interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida] >XP_038882491.1 interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida] >XP_038882492.1 interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 941.4 bits (2432), Expect = 4.8e-270
Identity = 532/590 (90.17%), Postives = 563/590 (95.42%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQTSKPKSLEAPLRKPRP RQLKAPGSDSVS S +PLP SKMTKERNPKT+VTKSVQSSV
Sbjct: 1   MQTSKPKSLEAPLRKPRPTRQLKAPGSDSVSVSPSPLPSSKMTKERNPKTIVTKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVSTMESQI QLQDELKKAKDQLN+SESGK+RAKEEAEE KQQL AMS EL++
Sbjct: 61  LEKKRPNRVSTMESQIAQLQDELKKAKDQLNASESGKRRAKEEAEEAKQQLNAMSSELKD 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQLLELSASEDERIQELRKIS DRDRAWQSELEALQKQHLMDSAALAAAM+ENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLS+YEDSEAQA++D+KK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSEYEDSEAQAVEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           TQMELETAN TIEML+SEG+ AMKAF+SISLELEQ KEKV SLEALVSKYQ+DLAEIDKK
Sbjct: 241 TQMELETANETIEMLQSEGTNAMKAFSSISLELEQSKEKVTSLEALVSKYQNDLAEIDKK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
           NLEDHSE+KNNDK++EENE++NQLK ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QI
Sbjct: 301 NLEDHSENKNNDKEDEENEYVNQLKTELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAEL QAKAQIEQLRT LEDKE QLLSIAK+NE +NLNQ
Sbjct: 361 RSSYEEVESIKSESRRKEAAFEAELIQAKAQIEQLRTHLEDKEAQLLSIAKENEIVNLNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           KSKE+E ESDLAEQLKK EADIE+LKASLLDKETELQGIIEENDMLRVDIQKM TERKI 
Sbjct: 421 KSKETENESDLAEQLKKLEADIEDLKASLLDKETELQGIIEENDMLRVDIQKMGTERKIE 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGK 721
           +GETTDLEE A+SANEEAL+KLGS+NENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEESARSANEEALNKLGSINENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 722 DGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           DGKLV+ISG IDSNYPL SFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDISGSIDSNYPLSSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 590

BLAST of Spg013274.1 vs. NCBI nr
Match: XP_008449280.1 (PREDICTED: interactor of constitutive active ROPs 2, chloroplastic [Cucumis melo] >XP_008449282.1 PREDICTED: interactor of constitutive active ROPs 2, chloroplastic [Cucumis melo])

HSP 1 Score: 913.7 bits (2360), Expect = 1.1e-261
Identity = 518/590 (87.80%), Postives = 556/590 (94.24%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQT KP+SLEAPLRKPRP+RQLK+PG+DSVSAS +PLPPSKMTKER+PKT V KSVQSSV
Sbjct: 1   MQTPKPRSLEAPLRKPRPIRQLKSPGTDSVSASVSPLPPSKMTKERSPKT-VAKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVST+ESQI QLQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+
Sbjct: 61  LEKKRPNRVSTVESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQLLELSASEDERIQELRKIS DRDRAWQSELEALQKQHLMDSAAL AAM+ENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKL 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SEANQSRHAESAHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           TQMELETAN TIE L+SEG+ AMKAF+SISLELEQ KEKV +LEALVSKYQDDLAEIDKK
Sbjct: 241 TQMELETANKTIERLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
           NLEDHSE+KNNDKD+EENE+INQL  ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QI
Sbjct: 301 NLEDHSENKNNDKDDEENEYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAEL QAK QI+QLRT LEDKE QL+S+AK+ ET +LNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           KSKESE E+D++EQLKKFE+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI 
Sbjct: 421 KSKESEIETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGK 721
           +GETTDLEEP KSANEE L+KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPTKSANEETLNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 722 DGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           DGKLV+I+G IDSNYPL S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Spg013274.1 vs. NCBI nr
Match: XP_011653597.1 (interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653599.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653600.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653602.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_031740135.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >KGN54185.1 hypothetical protein Csa_018051 [Cucumis sativus])

HSP 1 Score: 912.5 bits (2357), Expect = 2.4e-261
Identity = 518/590 (87.80%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQTSKPKSLE PLRKPRP+RQLK+PGSDSVSAS +PLPPSKMTKERNPKTVVTKSVQSSV
Sbjct: 1   MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVST+ESQI QLQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+
Sbjct: 61  LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQL +LSASEDERIQELRK+S DRDRAWQSELEALQKQHLMDSAALAAAM+ENQKLKL
Sbjct: 121 SRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SEANQSRHAESAHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           T MEL+TAN TIE L+SEG+ AMKAF+SISLELEQ KEKV +LEALVSKYQDD AEID+K
Sbjct: 241 THMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
            LEDHSE+KNNDKD+E NE+INQLK ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QI
Sbjct: 301 KLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAEL QAKAQI+QLRT LEDKE QLLSIAK+ ET +LNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           K KESE E+D++EQLKKFE+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI 
Sbjct: 421 KIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGK 721
           +GETTDLEEPAKSANEE  +KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 722 DGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           DGKLV+I+G IDSNYPL S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Spg013274.1 vs. NCBI nr
Match: KAG6604442.1 (Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905.6 bits (2339), Expect = 2.9e-259
Identity = 525/634 (82.81%), Postives = 558/634 (88.01%), Query Frame = 0

Query: 138 PGCFSEQFAVKLLLAFCSCGSESFGGEYQLFIHPAFILQLELQNMQTSKPKSLEAPLRKP 197
           PGCFS+QF          CGSE F G+YQLFI PAFILQ ELQNMQTSKPK+LEAPLRKP
Sbjct: 30  PGCFSKQFG--------CCGSERFEGKYQLFIQPAFILQQELQNMQTSKPKNLEAPLRKP 89

Query: 198 RPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTMESQI 257
           RP RQLKAPGSDSVSA +                    SVQSSVLEKKRPNRVSTMESQI
Sbjct: 90  RPTRQLKAPGSDSVSAPA--------------------SVQSSVLEKKRPNRVSTMESQI 149

Query: 258 VQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEESRQQLLELSASEDERI 317
            QLQDELKKAKDQLNS+E G+KRAKEEAEE +QQLEAMSLELEESRQQLLELSASEDERI
Sbjct: 150 AQLQDELKKAKDQLNSAELGRKRAKEEAEEGRQQLEAMSLELEESRQQLLELSASEDERI 209

Query: 318 QELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKLQLERIASSEANQSRHA 377
           QELRKIS DRDRAWQSELEALQKQHLMDSAAL AAMNENQKLKLQLERIA  E N SRHA
Sbjct: 210 QELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMNENQKLKLQLERIAGLEVNHSRHA 269

Query: 378 ESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKKTQMELETANATIEMLR 437
           ESA AEI  LR+ELKETLSLVEELKSKLS+YEDSEAQ+++D KKTQMELETAN TIE LR
Sbjct: 270 ESAQAEIHSLRVELKETLSLVEELKSKLSEYEDSEAQSVEDQKKTQMELETANETIEALR 329

Query: 438 SEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKKNLEDHSESKNNDKDNE 497
           SEG+ AMKAF+SISLELEQ KEKV SLEALVSKYQ DLAEIDKKNLED SE+KNN+KD E
Sbjct: 330 SEGTNAMKAFSSISLELEQSKEKVASLEALVSKYQVDLAEIDKKNLEDQSENKNNEKDEE 389

Query: 498 ENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQIRSSYEEVESVKSESRR 557
           E E+INQLK ELNCVRSEMGQL+LALDAADRRYQDEYLRST+QIRSSYEE+ESV+SESR 
Sbjct: 390 ETEYINQLKTELNCVRSEMGQLKLALDAADRRYQDEYLRSTIQIRSSYEELESVRSESRL 449

Query: 558 KEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQKSKESETESDLAEQLK 617
           +EAAFEAEL +AKAQIEQLRT LEDKE QLLSIAK+ ET NLNQ+SKESE ESD  EQLK
Sbjct: 450 REAAFEAELVRAKAQIEQLRTDLEDKEAQLLSIAKEKETSNLNQRSKESERESDNTEQLK 509

Query: 618 KFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIGNGETTDLEEPAKSANE 677
           K EADIEELKASLLDKETELQGIIEENDMLRVDIQKMET RK+ NGETTDLEE   +ANE
Sbjct: 510 KLEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETGRKMANGETTDLEE---AANE 569

Query: 678 EALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVEISGPIDSNYP 737
           E+L+KLGSVNENSEMEAELRRLRVQL+QWRKAAEAAAAMLSPGKDGKLV+ISGPIDSNYP
Sbjct: 570 ESLNKLGSVNENSEMEAELRRLRVQLEQWRKAAEAAAAMLSPGKDGKLVDISGPIDSNYP 629

Query: 738 LGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
            GS YSE+LDDDSPKKKNINMLKKIGVLWKK+QK
Sbjct: 630 HGSLYSEELDDDSPKKKNINMLKKIGVLWKKNQK 632

BLAST of Spg013274.1 vs. ExPASy Swiss-Prot
Match: Q9ZQC5 (Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICR2 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.1e-98
Identity = 272/634 (42.90%), Postives = 390/634 (61.51%), Query Frame = 0

Query: 182 MQTSKPK--SLEAPLRK-----PRPVRQLKAPGSDSVSASSAPL------PPSKMTKERN 241
           MQT KP+  SLE P +K     P+  R+LK   SD VS+ +  +       P  +   R+
Sbjct: 1   MQTPKPRPGSLEVPQKKSPASTPKTARKLKTSESDPVSSPNTKIRTPKTQSPKVVADRRS 60

Query: 242 PKTVVTKSVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEV 301
           P+T V +      ++KKR  +   + SQI QLQ+ELKKAK+QL++SE+ KK A+++AEE 
Sbjct: 61  PRTPVNE------IQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEET 120

Query: 302 KQQLEAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAA 361
           K              QQL+E++ASED RI ELRK+S +RD+AWQSELEA+Q+QH MDSAA
Sbjct: 121 K--------------QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAA 180

Query: 362 LAAAMNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDY 421
           L++ MNE QKLK QL               S    ++ LR+EL ETLSLVE+L+ +L D 
Sbjct: 181 LSSTMNEVQKLKAQL---------------SESENVENLRMELNETLSLVEKLRGELFDA 240

Query: 422 EDSEAQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALV 481
           ++ EAQA + +  T+ +LE AN T+EMLRS+G K  +A NS++ ELEQ K +V SLE LV
Sbjct: 241 KEGEAQAHEIVSGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLV 300

Query: 482 SKYQDDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADR 541
                       + LE+  E++ N   N ++  + +LK E+N  R E+ QL+ A++  +R
Sbjct: 301 ------------RQLEEEDEARGN--ANGDSSSVEELKEEINVARQEISQLKSAVEVTER 360

Query: 542 RYQDEYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLL 601
           RY +EY++STLQIR++YE+V+ VKS   ++EA    EL + KA+ + L  RL DKE +L 
Sbjct: 361 RYHEEYIQSTLQIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLR 420

Query: 602 SIAKDNETLNLNQKSKE----------SETESDLAEQLKKFEADIEELKASLLDKETELQ 661
            +  +NE LN   K KE          ++ E +   +LKK E+D+ EL+A+L+DKE ELQ
Sbjct: 421 ILVDENEILNSKIKEKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQ 480

Query: 662 GIIEENDMLRVDIQKMETER---------KIGNGETTDLEEPAKSANEEALDKLGSVN-E 721
            ++ + + LR +++ M++E+         K+G+  T + ++  K A E A ++LG+    
Sbjct: 481 SVMSQYESLRSEMETMQSEKNKAIDEALAKLGS-LTEEADKSGKRA-ENATEQLGAAQVT 540

Query: 722 NSEMEAELRRLRVQLDQWRKAAEAAAAMLSPG-----KDGKLVEISGPIDS-----NYPL 772
           N+E+EAELRRL+VQ DQWRKAAEAAA MLS G      +GK VE +G ++S     N  +
Sbjct: 541 NTELEAELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLESPLRRRNVNM 583

BLAST of Spg013274.1 vs. ExPASy Swiss-Prot
Match: Q9LSS5 (Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.3e-78
Identity = 242/605 (40.00%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 182 MQTSKPK--SLEAPLR-KPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQ 241
           MQT K +  S + P +  PR  R LK    +  S+SS     ++  K+++P  +  +S +
Sbjct: 1   MQTQKARNGSPDVPKKVSPRAARPLKIAALEPESSSSPISATNRTPKDKSPNVLNRRSPR 60

Query: 242 SSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLE 301
           S V EKKRP+R++ +E  + QLQ+ELKKAKDQ++ SE+ KK+A++EAEE ++QL+ +S +
Sbjct: 61  SPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSK 120

Query: 302 LEESRQQLLELSASEDE--RIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNEN 361
           LEES+ Q +E SA E+E  +   L   S+ ++  W+    A ++      A LAA  +E 
Sbjct: 121 LEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEI 180

Query: 362 QKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQAL 421
           ++LKLQ+E +ASSEA   + AE  ++E+Q LR  L +TL  VE  +++L D E SEA+  
Sbjct: 181 RQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETE 240

Query: 422 DDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLA 481
               +T  +LE A   +E L+S+G+KA++++  +++ELEQ K +++ LEALV+K Q++ A
Sbjct: 241 ALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNNPA 300

Query: 482 EIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLR 541
           +     LE+H E    D ++      N++  E++ +R E+ +LR AL+A+D++ Q+    
Sbjct: 301 D-----LENH-EILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQE---- 360

Query: 542 STLQIRSSYEEVESVKSESR-RKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNE 601
                        +V++ SR R +A  ++EL  AK++I++L+ RL DKET+L  I+++ +
Sbjct: 361 ------------GNVEASSRLRIQAELQSELKIAKSEIDELKARLMDKETELQFISEERD 420

Query: 602 TLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKME 661
             N + K  +++ E D+  +LKK    IE LKA L+DKETELQ + +EN+ L+ DI K E
Sbjct: 421 --NFSMKLMKNQKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSE 480

Query: 662 TE-----RKIGNGETTDLEEPAKSANE--EALDKLGSVN-ENSEMEAELRRLRVQLDQWR 721
           T+      K+G      +EE  KS+ +     ++L +    NSEME ELR+L+VQ +QWR
Sbjct: 481 TDVQDAFLKLG----IAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSNQWR 540

Query: 722 KAAEAAAAMLSPGKDGKLVEISGPIDSNY-PLGSFYSEDLDDDSPKKKNINMLKKIGVLW 772
           KAAEAA AMLS G +GK  E       NY    S YSED+DD+  KKKN N+LKKIGVLW
Sbjct: 541 KAAEAATAMLSAGNNGKFAE-------NYNQTNSPYSEDIDDELTKKKNGNVLKKIGVLW 564

BLAST of Spg013274.1 vs. ExPASy Swiss-Prot
Match: Q8VYU8 (Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 1.6e-45
Identity = 198/601 (32.95%), Postives = 286/601 (47.59%), Query Frame = 0

Query: 182 MQT--SKPKSLEAPLRK-----PRPVRQLKAPGSDSVSASSAPLPPSK-MTKERNPKTVV 241
           MQT  S+P SLE P +K     P+ VR+LK  G++S        P +K ++K + PK V 
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESD-------PKTKTISKTQIPKVVA 60

Query: 242 TK---SVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQ 301
            +    +  + ++KKR  R+  +ES I QLQ+ELKKAK++LN SE+ K+ A+EEAE+ K 
Sbjct: 61  DRRSARIPLNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAK- 120

Query: 302 QLEAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALA 361
                         QL++++ASED RI+ELRK+S +RD+ WQSELEA+Q+QH MDS AL+
Sbjct: 121 -------------HQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALS 180

Query: 362 AAMNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYED 421
           +A+NE                                          V++LKSKL + E 
Sbjct: 181 SAINE------------------------------------------VQKLKSKLFESES 240

Query: 422 SEAQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSK 481
                                                     ELEQ K +V SLE LV +
Sbjct: 241 ------------------------------------------ELEQSKYEVRSLEKLVRQ 300

Query: 482 YQDDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRY 541
            +               E + N +D+  +  + +LK  +N  R E+ QL+ A++AA+ RY
Sbjct: 301 LE---------------EERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRY 360

Query: 542 QDEYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSI 601
           Q+EY++STLQIRS+YE+ E+VKS   ++EA    EL + K +IE LR             
Sbjct: 361 QEEYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRK------------ 396

Query: 602 AKDNETLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVD 661
                   L +K KE E+  D    LKK E+D+ E++ SL+DKE ELQ       +LR  
Sbjct: 421 -------ELMEKVKEDESTGD----LKKLESDLMEVRGSLMDKEMELQ-------ILR-- 396

Query: 662 IQKMETERKIGNGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAA 721
                          + +E+  ++AN EA            MEAEL+R+++Q +QWRKAA
Sbjct: 481 ---------------SAMEKKVETANTEA------------MEAELKRVKIQCEQWRKAA 396

Query: 722 EAAAAMLSPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQ 772
           E AA++L+                         E+  D     K   MLKK GVL KK+ 
Sbjct: 541 ETAASILN-----------------------NDEERTDSIETSK---MLKKFGVLLKKNH 396

BLAST of Spg013274.1 vs. ExPASy Swiss-Prot
Match: F4I8B9 (Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g65010 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.1e-06
Identity = 149/596 (25.00%), Postives = 273/596 (45.81%), Query Frame = 0

Query: 185 SKPKSLEAPLRKPR--PVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSVL 244
           +K   +E P  KP   P R  K   S S S S++P+P ++++ +R+P TV +K       
Sbjct: 5   TKTGLMETPRSKPSPPPPRLSKLSASKSDSNSASPVPNTRLSLDRSPPTVNSKPTPD--- 64

Query: 245 EKKRPNRVST-------------MESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVK 304
             +RP+R+ T             +++Q+ Q+Q++LKKA +Q+   +  K +A ++ +E +
Sbjct: 65  --RRPSRIPTPEKVHSRLVKGTELQTQLNQIQEDLKKADEQIELLKKDKAKAIDDLKESE 124

Query: 305 QQLEAMSLELEESRQQLLELSASEDERIQELRKISLD---------RDRAWQSELEALQK 364
           + +E  + +L+E+     +  A E   +++ R + L+         +D   ++ELE+++ 
Sbjct: 125 KLVEEANEKLKEA--LAAQKRAEESFEVEKFRAVELEQAGLEAVQKKDVTSKNELESIRS 184

Query: 365 QHLMDSAALAAAMNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETL-SLVE 424
           QH +D +AL +   E Q++K +L   A ++     HAE A  +I  +  E  E L S + 
Sbjct: 185 QHALDISALLSTTEELQRVKHELSMTADAKNKALSHAEEA-TKIAEIHAEKAEILASELG 244

Query: 425 ELKSKLSDYEDSEA-QALDDMKKTQMELETANATIE---MLRSEGSKAMKAFNSISLELE 484
            LK+ L   E+ EA +  + + K + E+E     +E   +L S   +       + ++LE
Sbjct: 245 RLKALLGSKEEKEAIEGNEIVSKLKSEIELLRGELEKVSILESSLKEQEGLVEQLKVDLE 304

Query: 485 QYKEKVISLEALVSKYQDDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSE 544
             K       + V ++++ + E++K+ +E+ + SK++   +E  E + +  AELN V  E
Sbjct: 305 AAKMAESCTNSSVEEWKNKVHELEKE-VEESNRSKSS--ASESMESVMKQLAELNHVLHE 364

Query: 545 MGQLRLALDAADRRYQDEYLRSTLQI-RSSYEE------------------VESVKSESR 604
                   D A ++ + E L  T++  R+  EE                  VES+KSE  
Sbjct: 365 TKS-----DNAAQKEKIELLEKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELE 424

Query: 605 RKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSI----------AKDNETLNLNQKSKES 664
             +      L   KA    ++  L+ +    + +           KD E+L L    +E+
Sbjct: 425 ISQEEKTRALDNEKAATSNIQNLLDQRTELSIELERCKVEEEKSKKDMESLTL--ALQEA 484

Query: 665 ETESDLA--------EQLKKFEADIEELKASLLDKETE------LQGIIEENDMLR--VD 699
            TES  A        E+LK  E+ ++ LK  L  KET       L+    E D L+  VD
Sbjct: 485 STESSEAKATLLVCQEELKNCESQVDSLK--LASKETNEKYEKMLEDARNEIDSLKSTVD 544

BLAST of Spg013274.1 vs. ExPASy TrEMBL
Match: A0A5A7TWE3 (Interactor of constitutive active ROPs 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00780 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 1.3e-273
Identity = 541/632 (85.60%), Postives = 582/632 (92.09%), Query Frame = 0

Query: 140 CFSEQFAVKLLLAFCSCGSESFGGEYQLFIHPAFILQLELQNMQTSKPKSLEAPLRKPRP 199
           C+ +Q  VK          +SF GEYQLFI PAFILQ ELQNMQT KP+SLEAPLRKPRP
Sbjct: 10  CWGQQSTVK---------EKSFEGEYQLFIQPAFILQRELQNMQTPKPRSLEAPLRKPRP 69

Query: 200 VRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTMESQIVQ 259
           +RQLK+PG+DSVSAS +PLPPSKMTKER+PKT V KSVQSSVLEKKRPNRVST+ESQI Q
Sbjct: 70  IRQLKSPGTDSVSASVSPLPPSKMTKERSPKT-VAKSVQSSVLEKKRPNRVSTVESQIAQ 129

Query: 260 LQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEESRQQLLELSASEDERIQE 319
           LQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+SRQQLLELSASEDERIQE
Sbjct: 130 LQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLLELSASEDERIQE 189

Query: 320 LRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKLQLERIASSEANQSRHAES 379
           LRKIS DRDRAWQSELEALQKQHLMDSAAL AAM+ENQKLKLQLERIA SEANQSRHAES
Sbjct: 190 LRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKLQLERIAGSEANQSRHAES 249

Query: 380 AHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKKTQMELETANATIEMLRSE 439
           AHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KKTQMELETAN TIE L+SE
Sbjct: 250 AHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKTQMELETANKTIERLQSE 309

Query: 440 GSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKKNLEDHSESKNNDKDNEEN 499
           G+ AMKAF+SISLELEQ KEKV +LEALVSKYQDDLAEIDKKNLEDHSE+KNNDKD+EEN
Sbjct: 310 GTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKKNLEDHSENKNNDKDDEEN 369

Query: 500 EHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQIRSSYEEVESVKSESRRKE 559
           E+INQL  ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QIRSSYEEVES+KSESRRKE
Sbjct: 370 EYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKE 429

Query: 560 AAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQKSKESETESDLAEQLKKF 619
           AAFEAEL QAK QI+QLRT LEDKE QL+S+AK+ ET +LNQKSKESE E+D++EQLKKF
Sbjct: 430 AAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQKSKESEIETDISEQLKKF 489

Query: 620 EADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIGNGETTDLEEPAKSANEEA 679
           E+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI +GETTDLEEP KSANEE 
Sbjct: 490 ESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPTKSANEET 549

Query: 680 LDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVEISGPIDSNYPLG 739
           L+KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLV+I+G IDSNYPL 
Sbjct: 550 LNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLS 609

Query: 740 SFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 610 SYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 631

BLAST of Spg013274.1 vs. ExPASy TrEMBL
Match: A0A1S3BLP1 (interactor of constitutive active ROPs 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491205 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 5.2e-262
Identity = 518/590 (87.80%), Postives = 556/590 (94.24%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQT KP+SLEAPLRKPRP+RQLK+PG+DSVSAS +PLPPSKMTKER+PKT V KSVQSSV
Sbjct: 1   MQTPKPRSLEAPLRKPRPIRQLKSPGTDSVSASVSPLPPSKMTKERSPKT-VAKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVST+ESQI QLQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+
Sbjct: 61  LEKKRPNRVSTVESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQLLELSASEDERIQELRKIS DRDRAWQSELEALQKQHLMDSAAL AAM+ENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKL 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SEANQSRHAESAHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           TQMELETAN TIE L+SEG+ AMKAF+SISLELEQ KEKV +LEALVSKYQDDLAEIDKK
Sbjct: 241 TQMELETANKTIERLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
           NLEDHSE+KNNDKD+EENE+INQL  ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QI
Sbjct: 301 NLEDHSENKNNDKDDEENEYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAEL QAK QI+QLRT LEDKE QL+S+AK+ ET +LNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           KSKESE E+D++EQLKKFE+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI 
Sbjct: 421 KSKESEIETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGK 721
           +GETTDLEEP KSANEE L+KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPTKSANEETLNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 722 DGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           DGKLV+I+G IDSNYPL S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Spg013274.1 vs. ExPASy TrEMBL
Match: A0A0A0KX24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G292460 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 1.2e-261
Identity = 518/590 (87.80%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQTSKPKSLE PLRKPRP+RQLK+PGSDSVSAS +PLPPSKMTKERNPKTVVTKSVQSSV
Sbjct: 1   MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVST+ESQI QLQDELKK KDQLNSSESGK+RAKEEAEE KQ+L  MS ELE+
Sbjct: 61  LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQL +LSASEDERIQELRK+S DRDRAWQSELEALQKQHLMDSAALAAAM+ENQKLKL
Sbjct: 121 SRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SEANQSRHAESAHAEIQGLRIELKETLSLVEEL+SKLS+YEDSEAQAL+D+KK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           T MEL+TAN TIE L+SEG+ AMKAF+SISLELEQ KEKV +LEALVSKYQDD AEID+K
Sbjct: 241 THMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
            LEDHSE+KNNDKD+E NE+INQLK ELNCVRSEMGQLRLALDAADRRYQDEYLRST+QI
Sbjct: 301 KLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAEL QAKAQI+QLRT LEDKE QLLSIAK+ ET +LNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           K KESE E+D++EQLKKFE+DIEELKASLLDKETELQGIIEENDMLRV IQKMETERKI 
Sbjct: 421 KIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAMLSPGK 721
           +GETTDLEEPAKSANEE  +KLGSVNENSE EAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 722 DGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           DGKLV+I+G IDSNYPL S+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Spg013274.1 vs. ExPASy TrEMBL
Match: A0A6J1CRJ4 (interactor of constitutive active ROPs 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013664 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 2.1e-255
Identity = 519/611 (84.94%), Postives = 548/611 (89.69%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQSSV 241
           MQ SKPK+LEAP+RKPRP RQLKAPGSDSVSASS+ +PPSKMTKER PKTVV KSVQSSV
Sbjct: 1   MQASKPKNLEAPMRKPRPTRQLKAPGSDSVSASSSLVPPSKMTKERIPKTVVQKSVQSSV 60

Query: 242 LEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLELEE 301
           LEKKRPNRVSTMESQI QLQDELKKAKDQLNSSESGK+RAKEEAEE KQQL+AMS ELEE
Sbjct: 61  LEKKRPNRVSTMESQITQLQDELKKAKDQLNSSESGKRRAKEEAEEAKQQLKAMSSELEE 120

Query: 302 SRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQKLKL 361
           SRQQLLELSASEDERIQELRKIS DRDRAWQSELEAL KQHLMDSAALA +MNENQKLK+
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALHKQHLMDSAALAVSMNENQKLKV 180

Query: 362 QLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALDDMKK 421
           QLERIA SE NQSRHAESAHAEIQ LRIELKETLSLVEELK+KLSDYEDSE QAL+D+KK
Sbjct: 181 QLERIAGSEVNQSRHAESAHAEIQSLRIELKETLSLVEELKTKLSDYEDSETQALEDLKK 240

Query: 422 TQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAEIDKK 481
           TQMELETANATIEMLR EG+K MKAF+S+SLELE+ KEKV SLE LV KYQDDLAEI KK
Sbjct: 241 TQMELETANATIEMLRLEGTKDMKAFSSLSLELEESKEKVTSLEGLVCKYQDDLAEIAKK 300

Query: 482 NLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRSTLQI 541
           NLED SESKN+DKD+ ENE+ NQLK ELNCV SEM QLRLALDAADRRYQDEYLRSTLQI
Sbjct: 301 NLEDLSESKNDDKDDGENEYTNQLKTELNCVISEMDQLRLALDAADRRYQDEYLRSTLQI 360

Query: 542 RSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETLNLNQ 601
           RSSYEEVES+KSESRRKEAAFEAELTQAKAQIEQLRT L DKE QLLSIAK++ET N NQ
Sbjct: 361 RSSYEEVESIKSESRRKEAAFEAELTQAKAQIEQLRTHLIDKEAQLLSIAKEDETSNSNQ 420

Query: 602 KSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETERKIG 661
           KSK  E+ES+L+EQLKK EAD+EELKASLLDKETELQGIIEEND LRVDI+KMETE KI 
Sbjct: 421 KSK-GESESNLSEQLKKLEADVEELKASLLDKETELQGIIEENDTLRVDIKKMETEWKIT 480

Query: 662 NGETTDLEEPAKSANEEALDKLGSVNE---------------------NSEMEAELRRLR 721
           NGETTD+EEPAKSANEE LDKLGSV E                     NSEMEAELRRLR
Sbjct: 481 NGETTDMEEPAKSANEETLDKLGSVTEPNWSKRRGILETEQPETVQSKNSEMEAELRRLR 540

Query: 722 VQLDQWRKAAEAAAAMLSPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLK 772
           VQLDQWRKAAEAAAAMLSPGKDGK+VE+SGPIDSNYPLG FYSEDLDDDSPKKKNINMLK
Sbjct: 541 VQLDQWRKAAEAAAAMLSPGKDGKVVELSGPIDSNYPLGPFYSEDLDDDSPKKKNINMLK 600

BLAST of Spg013274.1 vs. ExPASy TrEMBL
Match: A0A6J1EDK6 (interactor of constitutive active ROPs 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433198 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 5.8e-253
Identity = 510/594 (85.86%), Postives = 546/594 (91.92%), Query Frame = 0

Query: 182 MQTSKPKSLEAPLRKPRPVRQLKAPGSDSV----SASSAPLPPSKMTKERNPKTVVTKSV 241
           MQTSKPK+LEAPLRKPRP+RQLKAPGSDSV    SAS + LPPSKMTKER+P+ VVTKSV
Sbjct: 1   MQTSKPKNLEAPLRKPRPIRQLKAPGSDSVSAPASASGSLLPPSKMTKERSPRPVVTKSV 60

Query: 242 QSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSL 301
           QSSVLEKKRPNRVSTMESQI QLQDELKKAKDQLNS+E G++RAKEEAEE +QQLEAMSL
Sbjct: 61  QSSVLEKKRPNRVSTMESQIAQLQDELKKAKDQLNSAELGRRRAKEEAEEGRQQLEAMSL 120

Query: 302 ELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNENQ 361
           ELEESRQQLLELSASEDERIQELRKIS DRDRAWQSELEALQKQHLMDSAAL AAMNENQ
Sbjct: 121 ELEESRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMNENQ 180

Query: 362 KLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQALD 421
           KLKLQLERIA  E N SRHAESA AEI  LR+ELKETLSLVEELKSKLS+YEDSEAQ+++
Sbjct: 181 KLKLQLERIAGLEVNHSRHAESAQAEIHSLRVELKETLSLVEELKSKLSEYEDSEAQSVE 240

Query: 422 DMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLAE 481
           D KKTQMELETAN TIE LRSEG+ AMKAF+SISLELEQ KEKV SLEALVSKYQ DLAE
Sbjct: 241 DQKKTQMELETANETIEALRSEGTNAMKAFSSISLELEQSKEKVASLEALVSKYQVDLAE 300

Query: 482 IDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLRS 541
           IDKKNLED SE+KNN+KD EE E+INQLK ELNCVRSEMGQL+LALDAADRRYQDEYLRS
Sbjct: 301 IDKKNLEDQSENKNNEKDEEETEYINQLKTELNCVRSEMGQLKLALDAADRRYQDEYLRS 360

Query: 542 TLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNETL 601
           T+QIRSSYEE+ESV+SESR +EAAFEAEL +AKAQIEQLRT L DKET+LLSIAK+ ET 
Sbjct: 361 TIQIRSSYEELESVRSESRLREAAFEAELVRAKAQIEQLRTDLVDKETRLLSIAKEKETS 420

Query: 602 NLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETE 661
           NLNQ+SKESE ESD  EQLKK EADIEELKASLLDKETELQGIIEENDMLRVDIQKMET 
Sbjct: 421 NLNQRSKESERESDNTEQLKKLEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETG 480

Query: 662 RKIGNGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEAAAAML 721
           RK+ NGETT+LEEP   ANEE L+KLGSVNENSEMEAELRRLRVQL+QWRKAAEAAAAML
Sbjct: 481 RKMTNGETTNLEEP---ANEETLNKLGSVNENSEMEAELRRLRVQLEQWRKAAEAAAAML 540

Query: 722 SPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           SPGKDGKLV+ISGPID+NYP GS YSE+LDDDSPKKKNINMLKKIGVLWKK+QK
Sbjct: 541 SPGKDGKLVDISGPIDTNYPHGSLYSEELDDDSPKKKNINMLKKIGVLWKKNQK 591

BLAST of Spg013274.1 vs. TAIR 10
Match: AT2G37080.1 (ROP interactive partner 3 )

HSP 1 Score: 361.7 bits (927), Expect = 1.5e-99
Identity = 272/634 (42.90%), Postives = 390/634 (61.51%), Query Frame = 0

Query: 182 MQTSKPK--SLEAPLRK-----PRPVRQLKAPGSDSVSASSAPL------PPSKMTKERN 241
           MQT KP+  SLE P +K     P+  R+LK   SD VS+ +  +       P  +   R+
Sbjct: 1   MQTPKPRPGSLEVPQKKSPASTPKTARKLKTSESDPVSSPNTKIRTPKTQSPKVVADRRS 60

Query: 242 PKTVVTKSVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEV 301
           P+T V +      ++KKR  +   + SQI QLQ+ELKKAK+QL++SE+ KK A+++AEE 
Sbjct: 61  PRTPVNE------IQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEET 120

Query: 302 KQQLEAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAA 361
           K              QQL+E++ASED RI ELRK+S +RD+AWQSELEA+Q+QH MDSAA
Sbjct: 121 K--------------QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAA 180

Query: 362 LAAAMNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDY 421
           L++ MNE QKLK QL               S    ++ LR+EL ETLSLVE+L+ +L D 
Sbjct: 181 LSSTMNEVQKLKAQL---------------SESENVENLRMELNETLSLVEKLRGELFDA 240

Query: 422 EDSEAQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALV 481
           ++ EAQA + +  T+ +LE AN T+EMLRS+G K  +A NS++ ELEQ K +V SLE LV
Sbjct: 241 KEGEAQAHEIVSGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLV 300

Query: 482 SKYQDDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADR 541
                       + LE+  E++ N   N ++  + +LK E+N  R E+ QL+ A++  +R
Sbjct: 301 ------------RQLEEEDEARGN--ANGDSSSVEELKEEINVARQEISQLKSAVEVTER 360

Query: 542 RYQDEYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLL 601
           RY +EY++STLQIR++YE+V+ VKS   ++EA    EL + KA+ + L  RL DKE +L 
Sbjct: 361 RYHEEYIQSTLQIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLR 420

Query: 602 SIAKDNETLNLNQKSKE----------SETESDLAEQLKKFEADIEELKASLLDKETELQ 661
            +  +NE LN   K KE          ++ E +   +LKK E+D+ EL+A+L+DKE ELQ
Sbjct: 421 ILVDENEILNSKIKEKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQ 480

Query: 662 GIIEENDMLRVDIQKMETER---------KIGNGETTDLEEPAKSANEEALDKLGSVN-E 721
            ++ + + LR +++ M++E+         K+G+  T + ++  K A E A ++LG+    
Sbjct: 481 SVMSQYESLRSEMETMQSEKNKAIDEALAKLGS-LTEEADKSGKRA-ENATEQLGAAQVT 540

Query: 722 NSEMEAELRRLRVQLDQWRKAAEAAAAMLSPG-----KDGKLVEISGPIDS-----NYPL 772
           N+E+EAELRRL+VQ DQWRKAAEAAA MLS G      +GK VE +G ++S     N  +
Sbjct: 541 NTELEAELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLESPLRRRNVNM 583

BLAST of Spg013274.1 vs. TAIR 10
Match: AT5G60210.1 (ROP interactive partner 5 )

HSP 1 Score: 293.9 bits (751), Expect = 3.8e-79
Identity = 242/605 (40.00%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 182 MQTSKPK--SLEAPLR-KPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQ 241
           MQT K +  S + P +  PR  R LK    +  S+SS     ++  K+++P  +  +S +
Sbjct: 1   MQTQKARNGSPDVPKKVSPRAARPLKIAALEPESSSSPISATNRTPKDKSPNVLNRRSPR 60

Query: 242 SSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLE 301
           S V EKKRP+R++ +E  + QLQ+ELKKAKDQ++ SE+ KK+A++EAEE ++QL+ +S +
Sbjct: 61  SPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSK 120

Query: 302 LEESRQQLLELSASEDE--RIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNEN 361
           LEES+ Q +E SA E+E  +   L   S+ ++  W+    A ++      A LAA  +E 
Sbjct: 121 LEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEI 180

Query: 362 QKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQAL 421
           ++LKLQ+E +ASSEA   + AE  ++E+Q LR  L +TL  VE  +++L D E SEA+  
Sbjct: 181 RQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETE 240

Query: 422 DDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLA 481
               +T  +LE A   +E L+S+G+KA++++  +++ELEQ K +++ LEALV+K Q++ A
Sbjct: 241 ALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNNPA 300

Query: 482 EIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLR 541
           +     LE+H E    D ++      N++  E++ +R E+ +LR AL+A+D++ Q+    
Sbjct: 301 D-----LENH-EILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQE---- 360

Query: 542 STLQIRSSYEEVESVKSESR-RKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNE 601
                        +V++ SR R +A  ++EL  AK++I++L+ RL DKET+L  I+++ +
Sbjct: 361 ------------GNVEASSRLRIQAELQSELKIAKSEIDELKARLMDKETELQFISEERD 420

Query: 602 TLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKME 661
             N + K  +++ E D+  +LKK    IE LKA L+DKETELQ + +EN+ L+ DI K E
Sbjct: 421 --NFSMKLMKNQKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSE 480

Query: 662 TE-----RKIGNGETTDLEEPAKSANE--EALDKLGSVN-ENSEMEAELRRLRVQLDQWR 721
           T+      K+G      +EE  KS+ +     ++L +    NSEME ELR+L+VQ +QWR
Sbjct: 481 TDVQDAFLKLG----IAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSNQWR 540

Query: 722 KAAEAAAAMLSPGKDGKLVEISGPIDSNY-PLGSFYSEDLDDDSPKKKNINMLKKIGVLW 772
           KAAEAA AMLS G +GK  E       NY    S YSED+DD+  KKKN N+LKKIGVLW
Sbjct: 541 KAAEAATAMLSAGNNGKFAE-------NYNQTNSPYSEDIDDELTKKKNGNVLKKIGVLW 564

BLAST of Spg013274.1 vs. TAIR 10
Match: AT5G60210.2 (ROP interactive partner 5 )

HSP 1 Score: 293.9 bits (751), Expect = 3.8e-79
Identity = 242/605 (40.00%), Postives = 373/605 (61.65%), Query Frame = 0

Query: 182 MQTSKPK--SLEAPLR-KPRPVRQLKAPGSDSVSASSAPLPPSKMTKERNPKTVVTKSVQ 241
           MQT K +  S + P +  PR  R LK    +  S+SS     ++  K+++P  +  +S +
Sbjct: 1   MQTQKARNGSPDVPKKVSPRAARPLKIAALEPESSSSPISATNRTPKDKSPNVLNRRSPR 60

Query: 242 SSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQLEAMSLE 301
           S V EKKRP+R++ +E  + QLQ+ELKKAKDQ++ SE+ KK+A++EAEE ++QL+ +S +
Sbjct: 61  SPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSSK 120

Query: 302 LEESRQQLLELSASEDE--RIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAAMNEN 361
           LEES+ Q +E SA E+E  +   L   S+ ++  W+    A ++      A LAA  +E 
Sbjct: 121 LEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSATAGER------AGLAAVAHEI 180

Query: 362 QKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSEAQAL 421
           ++LKLQ+E +ASSEA   + AE  ++E+Q LR  L +TL  VE  +++L D E SEA+  
Sbjct: 181 RQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAETE 240

Query: 422 DDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQDDLA 481
               +T  +LE A   +E L+S+G+KA++++  +++ELEQ K +++ LEALV+K Q++ A
Sbjct: 241 ALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNNPA 300

Query: 482 EIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQDEYLR 541
           +     LE+H E    D ++      N++  E++ +R E+ +LR AL+A+D++ Q+    
Sbjct: 301 D-----LENH-EILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQE---- 360

Query: 542 STLQIRSSYEEVESVKSESR-RKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAKDNE 601
                        +V++ SR R +A  ++EL  AK++I++L+ RL DKET+L  I+++ +
Sbjct: 361 ------------GNVEASSRLRIQAELQSELKIAKSEIDELKARLMDKETELQFISEERD 420

Query: 602 TLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQKME 661
             N + K  +++ E D+  +LKK    IE LKA L+DKETELQ + +EN+ L+ DI K E
Sbjct: 421 --NFSMKLMKNQKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSE 480

Query: 662 TE-----RKIGNGETTDLEEPAKSANE--EALDKLGSVN-ENSEMEAELRRLRVQLDQWR 721
           T+      K+G      +EE  KS+ +     ++L +    NSEME ELR+L+VQ +QWR
Sbjct: 481 TDVQDAFLKLG----IAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSNQWR 540

Query: 722 KAAEAAAAMLSPGKDGKLVEISGPIDSNY-PLGSFYSEDLDDDSPKKKNINMLKKIGVLW 772
           KAAEAA AMLS G +GK  E       NY    S YSED+DD+  KKKN N+LKKIGVLW
Sbjct: 541 KAAEAATAMLSAGNNGKFAE-------NYNQTNSPYSEDIDDELTKKKNGNVLKKIGVLW 564

BLAST of Spg013274.1 vs. TAIR 10
Match: AT3G53350.2 (ROP interactive partner 4 )

HSP 1 Score: 188.0 bits (476), Expect = 2.9e-47
Identity = 200/599 (33.39%), Postives = 286/599 (47.75%), Query Frame = 0

Query: 182 MQT--SKPKSLEAPLRK-----PRPVRQLKAPGSDSVSASSAPLPPSK-MTKERNPKTVV 241
           MQT  S+P SLE P +K     P+ VR+LK  G++S        P +K ++K + PK V 
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESD-------PKTKTISKTQIPKVVA 60

Query: 242 -TKSVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQL 301
             +S +  + EKKR  R+  +ES I QLQ+ELKKAK++LN SE+ K+ A+EEAE+ K   
Sbjct: 61  DRRSARIPLNEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAK--- 120

Query: 302 EAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAA 361
                       QL++++ASED RI+ELRK+S +RD+ WQSELEA+Q+QH MDS AL++A
Sbjct: 121 -----------HQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSA 180

Query: 362 MNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSE 421
           +NE                                          V++LKSKL + E   
Sbjct: 181 INE------------------------------------------VQKLKSKLFESES-- 240

Query: 422 AQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQ 481
                                                   ELEQ K +V SLE LV + +
Sbjct: 241 ----------------------------------------ELEQSKYEVRSLEKLVRQLE 300

Query: 482 DDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQD 541
                          E + N +D+  +  + +LK  +N  R E+ QL+ A++AA+ RYQ+
Sbjct: 301 ---------------EERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRYQE 360

Query: 542 EYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAK 601
           EY++STLQIRS+YE+ E+VKS   ++EA    EL + K +IE LR               
Sbjct: 361 EYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRK-------------- 394

Query: 602 DNETLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQ 661
                 L +K KE E+  D    LKK E+D+ E++ SL+DKE ELQ       +LR    
Sbjct: 421 -----ELMEKVKEDESTGD----LKKLESDLMEVRGSLMDKEMELQ-------ILR---- 394

Query: 662 KMETERKIGNGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEA 721
                        + +E+  ++AN EA            MEAEL+R+++Q +QWRKAAE 
Sbjct: 481 -------------SAMEKKVETANTEA------------MEAELKRVKIQCEQWRKAAET 394

Query: 722 AAAMLSPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           AA++L+                         E+  D     K   MLKK GVL KK+ K
Sbjct: 541 AASILN-----------------------NDEERTDSIETSK---MLKKFGVLLKKNHK 394

BLAST of Spg013274.1 vs. TAIR 10
Match: AT3G53350.3 (ROP interactive partner 4 )

HSP 1 Score: 188.0 bits (476), Expect = 2.9e-47
Identity = 200/599 (33.39%), Postives = 286/599 (47.75%), Query Frame = 0

Query: 182 MQT--SKPKSLEAPLRK-----PRPVRQLKAPGSDSVSASSAPLPPSK-MTKERNPKTVV 241
           MQT  S+P SLE P +K     P+ VR+LK  G++S        P +K ++K + PK V 
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESD-------PKTKTISKTQIPKVVA 60

Query: 242 -TKSVQSSVLEKKRPNRVSTMESQIVQLQDELKKAKDQLNSSESGKKRAKEEAEEVKQQL 301
             +S +  + EKKR  R+  +ES I QLQ+ELKKAK++LN SE+ K+ A+EEAE+ K   
Sbjct: 61  DRRSARIPLNEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAK--- 120

Query: 302 EAMSLELEESRQQLLELSASEDERIQELRKISLDRDRAWQSELEALQKQHLMDSAALAAA 361
                       QL++++ASED RI+ELRK+S +RD+ WQSELEA+Q+QH MDS AL++A
Sbjct: 121 -----------HQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSA 180

Query: 362 MNENQKLKLQLERIASSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSDYEDSE 421
           +NE                                          V++LKSKL + E   
Sbjct: 181 INE------------------------------------------VQKLKSKLFESES-- 240

Query: 422 AQALDDMKKTQMELETANATIEMLRSEGSKAMKAFNSISLELEQYKEKVISLEALVSKYQ 481
                                                   ELEQ K +V SLE LV + +
Sbjct: 241 ----------------------------------------ELEQSKYEVRSLEKLVRQLE 300

Query: 482 DDLAEIDKKNLEDHSESKNNDKDNEENEHINQLKAELNCVRSEMGQLRLALDAADRRYQD 541
                          E + N +D+  +  + +LK  +N  R E+ QL+ A++AA+ RYQ+
Sbjct: 301 ---------------EERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRYQE 360

Query: 542 EYLRSTLQIRSSYEEVESVKSESRRKEAAFEAELTQAKAQIEQLRTRLEDKETQLLSIAK 601
           EY++STLQIRS+YE+ E+VKS   ++EA    EL + K +IE LR               
Sbjct: 361 EYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRK-------------- 394

Query: 602 DNETLNLNQKSKESETESDLAEQLKKFEADIEELKASLLDKETELQGIIEENDMLRVDIQ 661
                 L +K KE E+  D    LKK E+D+ E++ SL+DKE ELQ       +LR    
Sbjct: 421 -----ELMEKVKEDESTGD----LKKLESDLMEVRGSLMDKEMELQ-------ILR---- 394

Query: 662 KMETERKIGNGETTDLEEPAKSANEEALDKLGSVNENSEMEAELRRLRVQLDQWRKAAEA 721
                        + +E+  ++AN EA            MEAEL+R+++Q +QWRKAAE 
Sbjct: 481 -------------SAMEKKVETANTEA------------MEAELKRVKIQCEQWRKAAET 394

Query: 722 AAAMLSPGKDGKLVEISGPIDSNYPLGSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 772
           AA++L+                         E+  D     K   MLKK GVL KK+ K
Sbjct: 541 AASILN-----------------------NDEERTDSIETSK---MLKKFGVLLKKNHK 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0047284.12.7e-27385.60interactor of constitutive active ROPs 2 [Cucumis melo var. makuwa][more]
XP_038882490.14.8e-27090.17interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida] >XP_... [more]
XP_008449280.11.1e-26187.80PREDICTED: interactor of constitutive active ROPs 2, chloroplastic [Cucumis melo... [more]
XP_011653597.12.4e-26187.80interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_01... [more]
KAG6604442.12.9e-25982.81Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argy... [more]
Match NameE-valueIdentityDescription
Q9ZQC52.1e-9842.90Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana ... [more]
Q9LSS55.3e-7840.00Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3... [more]
Q8VYU81.6e-4532.95Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5... [more]
F4I8B91.1e-0625.00Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A5A7TWE31.3e-27385.60Interactor of constitutive active ROPs 2 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BLP15.2e-26287.80interactor of constitutive active ROPs 2, chloroplastic OS=Cucumis melo OX=3656 ... [more]
A0A0A0KX241.2e-26187.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G292460 PE=3 SV=1[more]
A0A6J1CRJ42.1e-25584.94interactor of constitutive active ROPs 2, chloroplastic-like OS=Momordica charan... [more]
A0A6J1EDK65.8e-25385.86interactor of constitutive active ROPs 2, chloroplastic-like OS=Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
AT2G37080.11.5e-9942.90ROP interactive partner 3 [more]
AT5G60210.13.8e-7940.00ROP interactive partner 5 [more]
AT5G60210.23.8e-7940.00ROP interactive partner 5 [more]
AT3G53350.22.9e-4733.39ROP interactive partner 4 [more]
AT3G53350.32.9e-4733.39ROP interactive partner 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 250..312
NoneNo IPR availableCOILSCoilCoilcoord: 349..369
NoneNo IPR availableCOILSCoilCoilcoord: 480..500
NoneNo IPR availableCOILSCoilCoilcoord: 609..657
NoneNo IPR availableCOILSCoilCoilcoord: 689..716
NoneNo IPR availableCOILSCoilCoilcoord: 552..593
NoneNo IPR availableCOILSCoilCoilcoord: 384..439
NoneNo IPR availableCOILSCoilCoilcoord: 502..522
NoneNo IPR availableCOILSCoilCoilcoord: 454..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 481..500
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..221
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..259
NoneNo IPR availablePANTHERPTHR34224:SF4INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTICcoord: 688..771
coord: 182..690
IPR029688Interactor of constitutive active ROPsPANTHERPTHR34224INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATEDcoord: 688..771
coord: 182..690

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg013274Spg013274gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13466296..13466482CDS
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13466825..13466936CDS
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13467011..13467172CDS
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13467884..13467985CDS
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13468151..13468316CDS
Spg013274.1-cdsSpg013274.1-cds-scaffold1:13468675..13470261CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg013274.1-three_prime_utrSpg013274.1-three_prime_utr-scaffold1:13470262..13470563three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg013274.1Spg013274.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing