Spg012808.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg012808.1 vs. NCBI nr
Match: XP_038882187.1 (RNA cytosine C(5)-methyltransferase NSUN2-like [Benincasa hispida])

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 763/853 (89.45%), Postives = 797/853 (93.43%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKD----GNGNPWEPFESQSPAF 60
           MGGKGRGGRARTQRKHFR++RENVWKRP+ DPNS NDNKD    GNGNPWEPFESQSPAF
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRSDPNSANDNKDGNGNGNGNPWEPFESQSPAF 60

Query: 61  NEYYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVAD 120
           NEYYK QG+VS EEW+TFIEVLRKPLPAAFRINSSGQFC EIRAQLQNDFMKSLQAEV +
Sbjct: 61  NEYYKEQGIVSEEEWNTFIEVLRKPLPAAFRINSSGQFCDEIRAQLQNDFMKSLQAEVTE 120

Query: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180
           GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP
Sbjct: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQ 240
           PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIH+SSKPGDLP GLVVANDLDVQRCNLLIHQ
Sbjct: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHKSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDG 300
           TKRMCTANLIVTNHEAQHFPGCRA+ +FFNASAAG+E+KPHNTQLTFDRVLCDVPCSGDG
Sbjct: 241 TKRMCTANLIVTNHEAQHFPGCRAHTSFFNASAAGSEVKPHNTQLTFDRVLCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL
Sbjct: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360

Query: 361 LRKSGGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSG 420
           LRKSGGSLELVDVSNELPQLVCRPGLKKW+VRD+GVWLASYK++ E RQSVAIPSMFPSG
Sbjct: 361 LRKSGGSLELVDVSNELPQLVCRPGLKKWKVRDRGVWLASYKEIIEGRQSVAIPSMFPSG 420

Query: 421 RGRKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHD 480
           RGRKNQAD+NHNSELG NHLD S GSSVDVSEP   PV EFD EVCEFPIERC+RIVPHD
Sbjct: 421 RGRKNQADNNHNSELGGNHLDGSIGSSVDVSEPIMDPVLEFD-EVCEFPIERCIRIVPHD 480

Query: 481 QNSGAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDE 540
           QNSGAFFIAVLRKVAPLPAA EK   QNSKSA+DRSRCKLPQ   D E V+ +A   MDE
Sbjct: 481 QNSGAFFIAVLRKVAPLPAAVEK---QNSKSADDRSRCKLPQTIADGESVNVDAAVGMDE 540

Query: 541 SPSEIEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVD 600
           SPSE+ ID NNQ D +SADNKPAC+LPVQDI  ADGLD+NA DG  KQ SEADL   LVD
Sbjct: 541 SPSEVGIDPNNQEDFNSADNKPACELPVQDIKDADGLDTNAVDGRGKQFSEADLAAKLVD 600

Query: 601 -NEVSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAV 660
            NEV ENNLE+TAIKD SEESKQP DGEMDPKK++VKRKLQIQGKW+GVDPVVLFKDE V
Sbjct: 601 NNEVDENNLEVTAIKDNSEESKQPDDGEMDPKKSSVKRKLQIQGKWKGVDPVVLFKDETV 660

Query: 661 IDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIG 720
           IDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNF+VGQQLKITSIG
Sbjct: 661 IDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFAVGQQLKITSIG 720

Query: 721 LKMFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFV 780
           LKMFERQSSREGSSAPCSFRISSEGLPVILP+ITKQILT+SPVDFKHLLQYKSIKYADFV
Sbjct: 721 LKMFERQSSREGSSAPCSFRISSEGLPVILPHITKQILTMSPVDFKHLLQYKSIKYADFV 780

Query: 781 DAAFGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQE 840
           DAAFGEKASNLMLGCCVIVLGTGSNTSSD+IKVDESTIAIGCWKGRASLSVMVTA+DCQE
Sbjct: 781 DAAFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRASLSVMVTAIDCQE 840

Query: 841 LLGRLSNRLGNDL 848
           LLGRLS RL N+L
Sbjct: 841 LLGRLSKRLENNL 849

BLAST of Spg012808.1 vs. NCBI nr
Match: XP_022132778.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Momordica charantia])

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 757/850 (89.06%), Postives = 791/850 (93.06%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNG--NPWEPFESQSPAFNE 60
           MGGKGRGGRARTQRKHFRE+RENVWKRP+PDPNS NDNKDGNG  NPWEPF+SQSPAFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRENRENVWKRPRPDPNSGNDNKDGNGNTNPWEPFDSQSPAFNE 60

Query: 61  YYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGD 120
           YYK QG+V+AEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSL+AEVADGD
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLKAEVADGD 120

Query: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180
           EAEAIRPLPWYP+NLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPENLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTK 240
           FLDVHP+HYVLDMCAAPGSKTFQLLEIIH SSKPG LP GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPSHYVLDMCAAPGSKTFQLLEIIHLSSKPGGLPEGLVVANDLDVQRCNLLIHQTK 240

Query: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTL 300
           RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKP N+QL+FDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPQNSQLSFDRVLCDVPCSGDGTL 300

Query: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360
           RKAPDIWRKWN+GMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNAGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 361 KSGGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRG 420
           KS GSLELVDVSNELPQLV R GLKKWRVRDKGVWLASYKQV ECRQSV IPSMFPSGR 
Sbjct: 361 KSQGSLELVDVSNELPQLVRRSGLKKWRVRDKGVWLASYKQVNECRQSVVIPSMFPSGRS 420

Query: 421 RKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQN 480
           RKNQA+DNHNSELG   LD S GSSVDV++    P+HEFDEEVCEFP+ERCMRIVPHDQN
Sbjct: 421 RKNQAEDNHNSELGETRLDGSTGSSVDVADAAVDPLHEFDEEVCEFPLERCMRIVPHDQN 480

Query: 481 SGAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESP 540
           SGAFFIAVL KV+PLPAATEKP NQ SKSANDRS CKLPQ  TD + VD NA D MDESP
Sbjct: 481 SGAFFIAVLHKVSPLPAATEKPINQKSKSANDRSHCKLPQAITDVDSVDINAIDGMDESP 540

Query: 541 SEIEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNE 600
           SE+EID N QGD +SAD K   K PV+D+   DGLDS+AADG+DKQLSEA LE NL+DNE
Sbjct: 541 SEVEIDLNKQGDYNSADEKSPSKRPVEDVKDDDGLDSSAADGMDKQLSEAGLEDNLIDNE 600

Query: 601 VSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDS 660
           VSENNLEITAIKD +EESK+  DG  DP K+TVKRKLQIQGKW+GVDPVVLFKDE VIDS
Sbjct: 601 VSENNLEITAIKDKAEESKEADDGGTDPNKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720
           IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720

Query: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAA 780
           FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLL Y+SIKYADFVDAA
Sbjct: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLIYESIKYADFVDAA 780

Query: 781 FGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLG 840
           FGEKASNLMLGCCVIVLG GSN SSD+IKVDESTIAIGCWKGRASLSVM+TA+D QELLG
Sbjct: 781 FGEKASNLMLGCCVIVLGKGSNPSSDVIKVDESTIAIGCWKGRASLSVMLTAIDRQELLG 840

Query: 841 RLSNRLGNDL 848
           RLSNRL +DL
Sbjct: 841 RLSNRLEHDL 850

BLAST of Spg012808.1 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1498.4 bits (3878), Expect = 0.0e+00
Identity = 753/848 (88.80%), Postives = 789/848 (93.04%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NS ND KDGN N W PF+SQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+AEEWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSLQ+EV DGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVH NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+ NFFNASAAG EL  HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
            GSLELVDVSNELPQLV RPGLKKW+VRD+GVWLASYKQV++ RQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           NQAD +HNSEL  NHLD S GSSVDVSEPT  PVHEFD EVCEFPIERCMRIVPHDQNSG
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFFIAVLRKVAPLPAATEKPS QN K  ND+SRCKLPQ  T+   VD +A+  MDESPSE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           + IDSNNQGD +SADNKPAC+ PVQDI   +GLDSNAADGIDKQ SEADLEVNLVDNEVS
Sbjct: 541 VGIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDNEVS 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
            NNLEITAI+D  EESKQP DGEMDPKK+TVKRKLQIQGKW+GVDPVVLFKDE VIDSIK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF+VGQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPCSFRI+SEGLPVILPYITKQILT+SPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKASNLMLGCCVIVLGTGSNTSSD+IKVDESTIAIGCWKGR+SLSVMVTA+DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

Query: 841 SNRLGNDL 848
           SNRLGND+
Sbjct: 841 SNRLGNDV 847

BLAST of Spg012808.1 vs. NCBI nr
Match: XP_022950404.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1497.6 bits (3876), Expect = 0.0e+00
Identity = 750/848 (88.44%), Postives = 788/848 (92.92%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NS ND KDGN N W PF+SQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+A EWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSLQ+EV DGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+ N FNASAAG+EL PHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
            GSLELVDVSNELPQLV RPGLKKW+VRD+GVWLASYKQV++ RQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           NQAD +HNSEL  NHLD S GSSVDVSEPT  PVHEFD EVCEFPIERCMRIVPHDQNSG
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFFIAVLRKVAPLPAATEKPS QN K  ND+SRCKLPQ  T+   VD +A+  MDESPSE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           + +DSNNQGD +SADNKPAC+ PVQDI   +GLDSNAADGIDKQ SEADLEVNLVDNEVS
Sbjct: 541 VGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVS 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
            NNLEITAI+D  EESKQP DGEMDPKK+TVKRKLQIQGKW+GVDPVVLF+DE VIDSIK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF VGQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGS APCSFRI+SEGLPVILPYITKQILT+SPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKASNLMLGCCVIVLGTGSNTSSD+IKVDESTIAIGCWKGR+SLSVMVTA+DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

Query: 841 SNRLGNDL 848
           SNRLGND+
Sbjct: 841 SNRLGNDV 847

BLAST of Spg012808.1 vs. NCBI nr
Match: XP_022978420.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 748/848 (88.21%), Postives = 789/848 (93.04%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NS ND KDGN N W PF+SQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+AEEWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSLQ+EV DGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+ NFFNASAAG+EL  HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
            GSLE+VDVSNELPQLV RPGLKKW+VRD+GVWLASYKQV++ RQSVAIPSMFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           NQAD +HNSEL  NHL+ S GSSVDVSEPT  PVHEFD EVCEFPIERCMRIVPHDQNSG
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFFIAVLRKVAPLPAATEKPS  N K AND+SRCKLPQ  T+   VD +A+  MDESPSE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           + IDSNNQGD +SADNKPAC+ PVQDI   +GLDSNAADGIDKQ SEADLEVNL+DNEV+
Sbjct: 541 VGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVN 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
            NNLEITAI+D  EESKQP DGEMDPKK+TVKRKLQIQGKW+GVDPVVLFKDE VIDSIK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNFSVGQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPCSFRI+SEGLPVILPYITKQILT+SPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKAS+LMLGCCVIVLGTG NTSSD+IKVDESTIAIGCWKGR+SLSVMVTA+DCQELL RL
Sbjct: 781 EKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

Query: 841 SNRLGNDL 848
           SNRLGND+
Sbjct: 841 SNRLGNDV 847

BLAST of Spg012808.1 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 424.1 bits (1089), Expect = 3.7e-117
Identity = 283/820 (34.51%), Postives = 418/820 (50.98%), Query Frame = 0

Query: 18  RESRENVWKRPKPDPNSVNDNKDGNGN----PWE----PFESQSPAFNEYYKGQGVVSAE 77
           R +R   +++P P P    D  DG        WE        ++  F  YY+   +V   
Sbjct: 3   RRARGRRFQQP-PQPEGEEDASDGGRKRGQAGWEGGYPEIVKENKLFEHYYQELKIVPEG 62

Query: 78  EWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEAEAIRPLPWY 137
           EWD F+E LR+PLPA  RI        EI   L+N + K L+    DG + E  +PL WY
Sbjct: 63  EWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWY 122

Query: 138 PDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVL 197
           P+ LAWH+N SR  LRK+  L +FH+FL  E E GNI+RQEAVSM+PPL L+V P+H +L
Sbjct: 123 PEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKIL 182

Query: 198 DMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRMCTANLIVTN 257
           DMCAAPGSKT QL+E++H +      P G V+AND+D +RC LL+HQ KR+ +  ++V N
Sbjct: 183 DMCAAPGSKTTQLIEMLH-ADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVN 242

Query: 258 HEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRKAPDIWRKWN 317
           H+A   P    +++                 L +DR+LCDVPCSGDGT+RK  D+W+KW 
Sbjct: 243 HDASSIPRLTVDVD------------GRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWT 302

Query: 318 SGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSGGSLELVDV 377
           +     LHGLQ++I  RGA  L  GGRMVYSTCS+NPVE+EAV+A LL KS G+LEL DV
Sbjct: 303 TLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADV 362

Query: 378 SNELPQLVCRPGLKKWRVRDK-GVWLASYKQVTECRQSVAIPSMFPSGRGRKNQADDNHN 437
           S ELP L   PG+ +W+V  + G W A + +V + R +   P+MFP              
Sbjct: 363 SAELPGLKWMPGVSQWKVMTRDGQWFADWHEVPQGRHTQIRPTMFP-------------- 422

Query: 438 SELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSGAFFIAVLR 497
                               PT +      E++    +ERC+RI+PH QN+G FF+AVL 
Sbjct: 423 --------------------PTDL------EKLQAMHLERCLRILPHHQNTGGFFVAVLV 482

Query: 498 KVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVDNATDDMDESPSEIEIDSNNQG 557
           K AP+P    +P  QN KSA  R     P+ ++     +    D  E  S++       G
Sbjct: 483 KKAPMPWNKRQPKVQN-KSAEARE----PRVSSHVAATEGNPSDQSELESQM---ITGAG 542

Query: 558 DSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVSENNLEITAI 617
           DS +A N                                           +EN       
Sbjct: 543 DSETAHN-------------------------------------------TENT------ 602

Query: 618 KDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLF-KDEAVIDSIKTFYGINES 677
                ES +  DG   P  +   +K+++ G     DP V   +D+ +   I+ FY ++ S
Sbjct: 603 -----ESNEKKDGVCGPPPS---KKMKLFGFKE--DPFVFIPEDDPLFPPIEKFYALDPS 662

Query: 678 FPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFERQSSREGS 737
           FP + +L+TR ++    +++Y +SK +++VL  N    +++K+ + G+K++ R +S  G 
Sbjct: 663 FPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWCRNNS--GE 689

Query: 738 SAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFGEKASNLML 797
              C+FR++ EG+  + P+I  +I+T+S  D K LL  ++  +      A+ +   +L  
Sbjct: 723 EFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQ-VKDLAK 689

Query: 798 GCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMV 828
           G  V+     S     +    +  I +  W+G+AS+   V
Sbjct: 783 GSVVLKYEPDSANPDTL----QCPIVLCGWRGKASIRTFV 689

BLAST of Spg012808.1 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 416.4 bits (1069), Expect = 7.8e-115
Identity = 284/839 (33.85%), Postives = 417/839 (49.70%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGN----PWE----PFESQ 60
           MG + RG R + Q++                P    D  +G G      WE        +
Sbjct: 1   MGRRSRGRRLQQQQR----------------PEDAEDGAEGGGKRGEAGWEGGYPEIVKE 60

Query: 61  SPAFNEYYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQA 120
           +  F  YY+   +V   EW  F++ LR+PLPA  RI        EI   L+N + K L+ 
Sbjct: 61  NKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELED 120

Query: 121 EVADGDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAV 180
              DG + E  +PL WYP+ LAWH+N SR  LRK+  LE+FH+FL  E E GNI+RQEAV
Sbjct: 121 LEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAV 180

Query: 181 SMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNL 240
           SM+PPL L+V P+H +LDMCAAPGSKT QL+E++H        P G V+AND+D +RC L
Sbjct: 181 SMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYL 240

Query: 241 LIHQTKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPC 300
           L+HQ KR+ +  ++V NH+A   P  + +++                 L +DR+LCDVPC
Sbjct: 241 LVHQAKRLSSPCIMVVNHDASSIPRLQIDVD------------GRKEILFYDRILCDVPC 300

Query: 301 SGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAV 360
           SGDGT+RK  D+W+KW +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV
Sbjct: 301 SGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAV 360

Query: 361 VAELLRKSGGSLELVDVSNELPQLVCRPGLKKWRVRDK-GVWLASYKQVTECRQSVAIPS 420
           +A LL KS G+LEL DVSNELP L   PG+ +W+V  K G W   +  V   R +   P+
Sbjct: 361 IASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPT 420

Query: 421 MFPSGRGRKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMR 480
           MFP     K QA                                          +ERC+R
Sbjct: 421 MFPPKDPEKLQA----------------------------------------MHLERCLR 480

Query: 481 IVPHDQNSGAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKL-PQPTTDAECVDNAT 540
           I+PH QN+G FF+AVL K + +P    +P  Q  KSA  R   +L P   T+ +  D   
Sbjct: 481 ILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQ-GKSAETRESTQLSPADLTEGKPTD--- 540

Query: 541 DDMDESPSEIEIDS-NNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADL 600
                 PS++E  S    GD+  A             +A + L++N              
Sbjct: 541 ------PSKLESPSFTGTGDTEIA-------------HATEDLENNG------------- 600

Query: 601 EVNLVDNEVSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLF 660
                                    SK+ G     P K     K+++ G     DP V  
Sbjct: 601 -------------------------SKKDGVCGPPPSK-----KMKLFGFKE--DPFVFI 660

Query: 661 -KDEAVIDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQL 720
            +D+ +   I+ FY ++ SFP + +L+TR ++    +++Y +SK +++VL  N    +++
Sbjct: 661 PEDDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKM 690

Query: 721 KITSIGLKMFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSI 780
           K+ + G+K++ R +S  G    C+FR++ EG+  + P+I  +I+T+S  D K LL  ++ 
Sbjct: 721 KVINTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENP 690

Query: 781 KYADFVDAAFGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMV 828
            +       + + A +L  G  V+     S     +    +  I +  W+G+AS+   V
Sbjct: 781 FFRKLSSETYSQ-AKDLAKGSIVLKYEPDSANPDAL----QCPIVLCGWRGKASIRTFV 690

BLAST of Spg012808.1 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.3e-114
Identity = 277/824 (33.62%), Postives = 414/824 (50.24%), Query Frame = 0

Query: 7   GGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYYKGQGVV 66
           G RAR +R+  +  +    +          D++ G    +     ++  F  YY+ Q +V
Sbjct: 2   GRRARDRRRQLQPQQR---RERSGGGGGGGDDQAGWAGGYPEIVKENELFERYYREQRIV 61

Query: 67  SAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEAEAIRPL 126
              EWD F+  LR+PLPA  RI        EI   L+  + + LQ    DG + E  + L
Sbjct: 62  PDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQAL 121

Query: 127 PWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNH 186
            WYP+ LAWH+N SR  LRK+  LERFH+FL  E E GNI+RQEAVSM+PPL L+V+P+H
Sbjct: 122 SWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDH 181

Query: 187 YVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRMCTANLI 246
            +LDMCAAPGSKT QL+E++H        P G V+AND+D +RC LL+HQ KR+ +  ++
Sbjct: 182 KILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIM 241

Query: 247 VTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRKAPDIWR 306
           V NH+A   P  + +++                 L +DR+LCDVPCSGDGT+RK  D+W+
Sbjct: 242 VVNHDASSIPNLQVDVD------------GRKETLFYDRILCDVPCSGDGTMRKNIDVWK 301

Query: 307 KWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSGGSLEL 366
           KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL KS G+LEL
Sbjct: 302 KWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALEL 361

Query: 367 VDVSNELPQLVCRPGLKKWRVRDK-GVWLASYKQVTECRQSVAIPSMFPSGRGRKNQADD 426
            DVS+ELP L   PG+ KW+V  K G W   +K V   RQ+   P+MFP           
Sbjct: 362 ADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFP----------- 421

Query: 427 NHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSGAFFIA 486
                               + E         +E++    +ERC+RI+PH QN+G FF+A
Sbjct: 422 --------------------IKE---------EEKLKAMNLERCIRILPHHQNTGGFFVA 481

Query: 487 VLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVDNATDDMDESPSEIEIDSN 546
           VL K +P+P    +P          +   KLP  T D                  E+ + 
Sbjct: 482 VLIKKSPMPWNKRQP----------KVHQKLPGKTEDT-----------------EVTAT 541

Query: 547 NQGD-SSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVSENNLE 606
           N GD S  A  KP                  A D   K++ E                  
Sbjct: 542 NAGDGSEDATEKPTL----------------AEDEEPKKVQELQ---------------- 601

Query: 607 ITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDP-VVLFKDEAVIDSIKTFYG 666
                  + +++Q   G   P  +   +K+++ G     DP V L +D+ +   I+ FY 
Sbjct: 602 -------NSDTEQSKKGVCGPPPS---KKMKLFGFKE--DPFVFLPEDDPLFLPIQKFYA 661

Query: 667 INESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFERQSS 726
           ++ SFP + +L+TR  +    +++Y +SK +++VL  N    +++K+ + G+K++ R S 
Sbjct: 662 LDPSFPKM-NLLTRTQE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWSRNS- 686

Query: 727 REGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFGEKAS 786
            +G    C+FR++ EG+  + P+I  +I+ +   D K LL  ++   + F  +    K  
Sbjct: 722 -DGEQFGCAFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKF-SSETQRKVK 686

Query: 787 NLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMV 828
           ++ +G  V+          D+    +  I +  W+G+ SL   V
Sbjct: 782 DMAMGSIVLKYEPDPEKPDDL----QCPIVLCGWQGKTSLRAFV 686

BLAST of Spg012808.1 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.4e-110
Identity = 276/835 (33.05%), Postives = 406/835 (48.62%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWE----PFESQSPAF 60
           MG + R  R R QR   + S     +R K    +           WE        ++  F
Sbjct: 1   MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAA----------WECGYPEIIKENKLF 60

Query: 61  NEYYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVAD 120
             YY+   +V   EWD F+  LR+PLPA  RI        EI   L+  + K LQ    D
Sbjct: 61  EHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVD 120

Query: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180
           G + EA +PL WYP+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+P
Sbjct: 121 GQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQ 240
           P+ L V P+H +LDMCAAPGSKT Q++E++H        P G V+AND+D +RC LL+HQ
Sbjct: 181 PVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDG 300
            KR+ +  ++V NH+A   P      N         E+      L +DR+LCDVPCSGDG
Sbjct: 241 AKRLNSPCIMVVNHDASSIPRLLVENN------GSREV------LYYDRILCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRK  D+W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+A L
Sbjct: 301 TLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASL 360

Query: 361 LRKSGGSLELVDVSNELPQLVCRPGLKKWRVRDK-GVWLASYKQVTECRQSVAIPSMFPS 420
           L KS GSLEL DV++E+P L   PG+ +W+V  K G W   ++ +   R +   P+MFP 
Sbjct: 361 LDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPP 420

Query: 421 GRGRKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPH 480
                                                   + +E++    + RCMRI+PH
Sbjct: 421 ----------------------------------------KDEEKLKSMNLNRCMRILPH 480

Query: 481 DQNSGAFFIAVLRKVAPLPAATEKPSNQNSK--SANDRSRCKLPQPTTDAECVDNATDDM 540
            QN+G FF+AVL K AP+P    +P  Q     SA D S    P      E V   T++ 
Sbjct: 481 HQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAP------ELVKAVTENS 540

Query: 541 DESPSEIEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNL 600
                E  +D+ N       + KP                                    
Sbjct: 541 AGMADEPAVDTEN------GETKPC----------------------------------- 600

Query: 601 VDNEVSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDP-VVLFKDE 660
             N+   +  +I      S++ K  G  E                     DP V + +D+
Sbjct: 601 -TNQSDSSKTDIVCCPPPSKKMKLFGFKE---------------------DPFVFVSEDD 660

Query: 661 AVIDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITS 720
            + D I+TFY ++ SFP   +L+TR  +    +++Y +SK +++VL  N    +++K+ +
Sbjct: 661 PIFDPIQTFYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVIN 691

Query: 721 IGLKMFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYAD 780
            G+K+  R  + +G    C++R++ EG+  + P+I  +I+T+S  D K LL  ++   + 
Sbjct: 721 TGIKVLCR--NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSK 691

Query: 781 FVDAAFGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMV 828
           F      ++A+N  +G  V+           +    +  I +  W+G+ S+   V
Sbjct: 781 F-SKETQKQANNFDMGSIVLKYEPDPQEPETL----QCPIVLCGWRGKTSIRSFV 691

BLAST of Spg012808.1 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.9e-108
Identity = 274/834 (32.85%), Postives = 403/834 (48.32%), Query Frame = 0

Query: 1   MGGKGRGGRAR--TQRKHFRESRENVWKRPKPD---PNSVNDNKDGNGNPWEPFESQSPA 60
           MG K R  R R   QR    E R    ++   +   P  + +NK                
Sbjct: 1   MGRKNRRNRQRRTEQRSPAEEERRKAREQAAWEGGYPEIIKENK---------------L 60

Query: 61  FNEYYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVA 120
           F  YY+   +V   EWD F++ LR+PLPA  RI        EI   L+  + K L     
Sbjct: 61  FEHYYQELKIVPDGEWDKFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEV 120

Query: 121 DGDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 180
           DG + EA +PL WYP+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+
Sbjct: 121 DGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMI 180

Query: 181 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIH 240
           PP+ L+V P+H +LDMCAAPGSKT Q++E++H        P G V+AND+D +RC LL+H
Sbjct: 181 PPVLLNVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVH 240

Query: 241 QTKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGD 300
           Q KR+ +  ++V NH+A   P      N         E+      L +DR+LCDVPCSGD
Sbjct: 241 QAKRLNSPCIMVVNHDASSIPRLLIENN------GSREV------LYYDRILCDVPCSGD 300

Query: 301 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 360
           GT+RK  D+W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  
Sbjct: 301 GTMRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVS 360

Query: 361 LLRKSGGSLELVDVSNELPQLVCRPGLKKWRVRDK-GVWLASYKQVTECRQSVAIPSMFP 420
           LL KS GSLEL DV++ELP L   PG+ +WRV  K G W   ++ V   R +   P+MFP
Sbjct: 361 LLDKSEGSLELADVASELPGLKWMPGITQWRVMTKEGQWFEKWEDVPTSRHTQIRPTMFP 420

Query: 421 SGRGRKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVP 480
                                L D                   +E++    + RCMRI+P
Sbjct: 421 ---------------------LKD-------------------EEKLKSMNLNRCMRILP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVDNATDDMD 540
           H QN+G FF+AVL K AP+P    +P  Q     +       P+       +  A  D+ 
Sbjct: 481 HHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSVCDASVAPE-------IVKAVADIS 540

Query: 541 ESPSEIEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLV 600
               E  +D+ N       + KP                                     
Sbjct: 541 AIADEPAVDAEN------GETKPC------------------------------------ 600

Query: 601 DNEVSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDP-VVLFKDEA 660
            N+   +  +       S++ K  G  E                     DP V L +D+ 
Sbjct: 601 TNQSGSSKTDSVCCPPPSKKMKLFGFKE---------------------DPFVFLSEDDP 660

Query: 661 VIDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSI 720
           + + I+ FY ++ SFP   +L+TR  +    +++Y +SK +++VL  N    +++K+ + 
Sbjct: 661 IFEPIQKFYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVINT 684

Query: 721 GLKMFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADF 780
           G+K+  R  + +G    C++R++ EG+  + P+I  +ILT+S  D K LL  ++   + F
Sbjct: 721 GIKVLCR--NNDGEQYGCAYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQENPFLSKF 684

Query: 781 VDAAFGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMV 828
                 ++A+NL +G  V+           +    +  I +  W+G+ S+   V
Sbjct: 781 -SKETQKQANNLDMGSIVLKYEPDPQQPETL----QCPIVLCGWRGKTSIRSFV 684

BLAST of Spg012808.1 vs. ExPASy TrEMBL
Match: A0A6J1BXA3 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005554 PE=3 SV=1)

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 757/850 (89.06%), Postives = 791/850 (93.06%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNG--NPWEPFESQSPAFNE 60
           MGGKGRGGRARTQRKHFRE+RENVWKRP+PDPNS NDNKDGNG  NPWEPF+SQSPAFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRENRENVWKRPRPDPNSGNDNKDGNGNTNPWEPFDSQSPAFNE 60

Query: 61  YYKGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGD 120
           YYK QG+V+AEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSL+AEVADGD
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLKAEVADGD 120

Query: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180
           EAEAIRPLPWYP+NLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPENLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTK 240
           FLDVHP+HYVLDMCAAPGSKTFQLLEIIH SSKPG LP GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPSHYVLDMCAAPGSKTFQLLEIIHLSSKPGGLPEGLVVANDLDVQRCNLLIHQTK 240

Query: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTL 300
           RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKP N+QL+FDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPQNSQLSFDRVLCDVPCSGDGTL 300

Query: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360
           RKAPDIWRKWN+GMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNAGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 361 KSGGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRG 420
           KS GSLELVDVSNELPQLV R GLKKWRVRDKGVWLASYKQV ECRQSV IPSMFPSGR 
Sbjct: 361 KSQGSLELVDVSNELPQLVRRSGLKKWRVRDKGVWLASYKQVNECRQSVVIPSMFPSGRS 420

Query: 421 RKNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQN 480
           RKNQA+DNHNSELG   LD S GSSVDV++    P+HEFDEEVCEFP+ERCMRIVPHDQN
Sbjct: 421 RKNQAEDNHNSELGETRLDGSTGSSVDVADAAVDPLHEFDEEVCEFPLERCMRIVPHDQN 480

Query: 481 SGAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESP 540
           SGAFFIAVL KV+PLPAATEKP NQ SKSANDRS CKLPQ  TD + VD NA D MDESP
Sbjct: 481 SGAFFIAVLHKVSPLPAATEKPINQKSKSANDRSHCKLPQAITDVDSVDINAIDGMDESP 540

Query: 541 SEIEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNE 600
           SE+EID N QGD +SAD K   K PV+D+   DGLDS+AADG+DKQLSEA LE NL+DNE
Sbjct: 541 SEVEIDLNKQGDYNSADEKSPSKRPVEDVKDDDGLDSSAADGMDKQLSEAGLEDNLIDNE 600

Query: 601 VSENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDS 660
           VSENNLEITAIKD +EESK+  DG  DP K+TVKRKLQIQGKW+GVDPVVLFKDE VIDS
Sbjct: 601 VSENNLEITAIKDKAEESKEADDGGTDPNKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720
           IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720

Query: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAA 780
           FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLL Y+SIKYADFVDAA
Sbjct: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLIYESIKYADFVDAA 780

Query: 781 FGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLG 840
           FGEKASNLMLGCCVIVLG GSN SSD+IKVDESTIAIGCWKGRASLSVM+TA+D QELLG
Sbjct: 781 FGEKASNLMLGCCVIVLGKGSNPSSDVIKVDESTIAIGCWKGRASLSVMLTAIDRQELLG 840

Query: 841 RLSNRLGNDL 848
           RLSNRL +DL
Sbjct: 841 RLSNRLEHDL 850

BLAST of Spg012808.1 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1497.6 bits (3876), Expect = 0.0e+00
Identity = 750/848 (88.44%), Postives = 788/848 (92.92%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NS ND KDGN N W PF+SQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+A EWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSLQ+EV DGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+ N FNASAAG+EL PHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
            GSLELVDVSNELPQLV RPGLKKW+VRD+GVWLASYKQV++ RQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           NQAD +HNSEL  NHLD S GSSVDVSEPT  PVHEFD EVCEFPIERCMRIVPHDQNSG
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFFIAVLRKVAPLPAATEKPS QN K  ND+SRCKLPQ  T+   VD +A+  MDESPSE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           + +DSNNQGD +SADNKPAC+ PVQDI   +GLDSNAADGIDKQ SEADLEVNLVDNEVS
Sbjct: 541 VGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVS 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
            NNLEITAI+D  EESKQP DGEMDPKK+TVKRKLQIQGKW+GVDPVVLF+DE VIDSIK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNF VGQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGS APCSFRI+SEGLPVILPYITKQILT+SPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKASNLMLGCCVIVLGTGSNTSSD+IKVDESTIAIGCWKGR+SLSVMVTA+DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

Query: 841 SNRLGNDL 848
           SNRLGND+
Sbjct: 841 SNRLGNDV 847

BLAST of Spg012808.1 vs. ExPASy TrEMBL
Match: A0A6J1IQ20 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 748/848 (88.21%), Postives = 789/848 (93.04%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NS ND KDGN N W PF+SQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+AEEWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSLQ+EV DGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+ NFFNASAAG+EL  HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
            GSLE+VDVSNELPQLV RPGLKKW+VRD+GVWLASYKQV++ RQSVAIPSMFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           NQAD +HNSEL  NHL+ S GSSVDVSEPT  PVHEFD EVCEFPIERCMRIVPHDQNSG
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFFIAVLRKVAPLPAATEKPS  N K AND+SRCKLPQ  T+   VD +A+  MDESPSE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           + IDSNNQGD +SADNKPAC+ PVQDI   +GLDSNAADGIDKQ SEADLEVNL+DNEV+
Sbjct: 541 VGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVN 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
            NNLEITAI+D  EESKQP DGEMDPKK+TVKRKLQIQGKW+GVDPVVLFKDE VIDSIK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLELNFSVGQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPCSFRI+SEGLPVILPYITKQILT+SPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKAS+LMLGCCVIVLGTG NTSSD+IKVDESTIAIGCWKGR+SLSVMVTA+DCQELL RL
Sbjct: 781 EKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

Query: 841 SNRLGNDL 848
           SNRLGND+
Sbjct: 841 SNRLGNDV 847

BLAST of Spg012808.1 vs. ExPASy TrEMBL
Match: A0A6J1KLF5 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111496800 PE=3 SV=1)

HSP 1 Score: 1483.0 bits (3838), Expect = 0.0e+00
Identity = 756/848 (89.15%), Postives = 781/848 (92.10%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNS NDNKDGNGN WEPFESQSPAFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFESQSPAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+AEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDF +SLQAEV DGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFSRSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRAN NFF+AS AGNELKP +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
           GGSLELVDVSNELPQLV RPGLKKW+V DKGVW ASY+QVTE  QSVAIP MFPSGRG K
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGHK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           +   D HNS+L  NH D   GSSVDVSEPT  PVH FDEEVCEFPIERCMRIVPHDQN+G
Sbjct: 421 S---DTHNSQLRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFF+AVLRKVAPL AATEK S+QN+KSAND+SR KL Q  TDA+ VD NATD M ES SE
Sbjct: 481 AFFVAVLRKVAPLLAATEKTSDQNTKSANDQSRGKLQQTITDADSVDLNATDGMGESSSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           +EID NNQ DS+SA+N P CKLPVQD   ADGLDS   DG+DK  SEADLEV  VDNE +
Sbjct: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKPFSEADLEV--VDNETT 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
           EN LEITAIK+ SEESKQPGDGEMDPKK   KRKLQIQGKWRGVDPVVLFKDE VID IK
Sbjct: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFS GQQLKITSIGLKMFE
Sbjct: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPC+FRISSEGLPV+LPYITKQILT+SPVDFKHLLQYKSIKYADFVDAAFG
Sbjct: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKASNLMLGCCVIVLGTG NTSSD+IKVDESTIAIGCWKGRASLSVMVTA DCQELLGRL
Sbjct: 781 EKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 838

Query: 841 SNRLGNDL 848
           SNRLGNDL
Sbjct: 841 SNRLGNDL 838

BLAST of Spg012808.1 vs. ExPASy TrEMBL
Match: A0A6J1HGN2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463320 PE=3 SV=1)

HSP 1 Score: 1481.1 bits (3833), Expect = 0.0e+00
Identity = 755/848 (89.03%), Postives = 780/848 (91.98%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNS N NKDGNGN WEPFESQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60

Query: 61  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 120
           K QG+V+AEEWDTFIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF KSLQAEV DGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRAN NFF+AS AGNELKP +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 420
           GGSLELVDVSNELPQLV RPGLKKW+V DKGVW ASY+QVTE  QSVAIP MFPSGRGRK
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420

Query: 421 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 480
           +   D HNSEL  NH D   GSSVDVSEPT  PVH FDEEVCEFPIERCMRIVPHDQN+G
Sbjct: 421 S---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVD-NATDDMDESPSE 540
           AFF+AVLRKVAPLPAATEK S+QN+KSAND+S  KL Q  TDA+ VD NATD M ES SE
Sbjct: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540

Query: 541 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 600
           +EID NNQ DS+SA+N P CKLPVQD   ADGLDS   DG+DKQ SEAD+EV  V+NE +
Sbjct: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENETT 600

Query: 601 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 660
           EN LEITAIK+ SEESKQPGDGEMDPKK   KRKLQIQGKWRGVDPVVLFKDE VID IK
Sbjct: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMFE 720
           TFYGINESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFS GQQLKITSIGLKMFE
Sbjct: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPC+FRISSEGLPV+LPYITKQILT+SPVDFKHLLQYKSIKYADFVDAAFG
Sbjct: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780

Query: 781 EKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGRL 840
           EKASNLMLGCCVIVLGTG NTSSD+IKVDESTIAIGCWKGRASLSVMVTA DCQELLGRL
Sbjct: 781 EKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 838

Query: 841 SNRLGNDL 848
           SNRLGNDL
Sbjct: 841 SNRLGNDL 838

BLAST of Spg012808.1 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 927.2 bits (2395), Expect = 9.7e-270
Identity = 497/844 (58.89%), Postives = 606/844 (71.80%), Query Frame = 0

Query: 5   GRGG--RARTQRKHFRESRENVWKRPKPDPN-SVNDNKDGNGNP-WEPFESQSPAFNEYY 64
           GRGG  R RTQRK F+ESRENVWKRPK D +   +DN      P WEP  + +P F EYY
Sbjct: 2   GRGGRHRGRTQRKDFKESRENVWKRPKSDASVDGSDNAVPEQKPTWEPIVTVNPNFEEYY 61

Query: 65  KGQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEA 124
           K QG+V AEEWD F+E+LRKPLPAAFR+NS+GQFC EI + L+NDFMKSLQAE  +G E 
Sbjct: 62  KKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAIEGGEL 121

Query: 125 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 184
           EAI+PLPWYP NLAWHSNFSR ++RKNQ LERFHEFLKLENE+GNITRQEAVSMVPPLFL
Sbjct: 122 EAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMVPPLFL 181

Query: 185 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRM 244
           DVHP+H+VLDMCAAPGSKTFQLLEIIH++S+PG LP+GLVVAND+D +R NLLIHQTKRM
Sbjct: 182 DVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIHQTKRM 241

Query: 245 CTANLIVTNHEAQHFPGCRANINFFNASAAG-NELKPHNTQLTFDRVLCDVPCSGDGTLR 304
           CT+NLIVTNHE Q FPGCR  +N   AS  G +E  P N QL FDRVLCDVPCSGDGTLR
Sbjct: 242 CTSNLIVTNHEGQQFPGCR--LNKSRASEKGISENMPIN-QLAFDRVLCDVPCSGDGTLR 301

Query: 305 KAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRK 364
           KAPDIWRKWNSGMGNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE+LR+
Sbjct: 302 KAPDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAEILRR 361

Query: 365 SGGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGR 424
            G S+EL+DVS++LP+L+ RPGLK W+VRDKG W  SYK V + R+   + SMFPSG+  
Sbjct: 362 CGDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPSGKYL 421

Query: 425 KNQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNS 484
           K+  +    +E G  +  +      D+S     P  E  EEV + P+ERCMRI+PHDQN+
Sbjct: 422 KDSTETTEKNENGDVNGCEDGLKETDISVVDATP-EEQAEEVSDLPLERCMRIIPHDQNT 481

Query: 485 GAFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVDNATDDMDESPSE 544
           GAFFIAVL+K +PLP   EKP+ + + +A                    +TD  ++SPS+
Sbjct: 482 GAFFIAVLQKKSPLPEFQEKPNTKRNSTA-------------------KSTDSTEKSPSK 541

Query: 545 IEIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVS 604
             + + + G              V D +A +            ++ EAD   N+  N+  
Sbjct: 542 ESVVTVDAG--------------VPDESAVE------------KVIEAD--SNIEKNDSL 601

Query: 605 ENNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQIQGKWRGVDPVVLFKDEAVIDSIK 664
           E   +IT  +  +E      D E +      KRK+ +QGKW+G DPVV  KDE VI+ IK
Sbjct: 602 EPEKKITEGESITE------DKEANSSNAGGKRKVPMQGKWKGFDPVVFVKDETVINGIK 661

Query: 665 TFYGI-NESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMF 724
            FYGI +ESFPL GHLV RN+DT+ VKRIYY+SKSVK+VL+LNF+VGQQLKI S+GLKMF
Sbjct: 662 EFYGIKDESFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVGQQLKIASVGLKMF 721

Query: 725 ERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADFVDAAF 784
           ERQS++EGSS  C FRISSEGLPVILPYITKQ+L     DFK LLQ KSIK+ DFV+   
Sbjct: 722 ERQSAKEGSSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQDKSIKFHDFVNPQL 781

Query: 785 GEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQELLGR 843
            +KA++L++G CV++L  G       +K D STIAI CW+G+ASL+VM T  DCQELL R
Sbjct: 782 AQKATDLVMGSCVVILSDGEVP----VKADASTIAISCWRGKASLAVMATVADCQELLER 784

BLAST of Spg012808.1 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 835.9 bits (2158), Expect = 2.9e-242
Identity = 457/849 (53.83%), Postives = 574/849 (67.61%), Query Frame = 0

Query: 3   GKGRGGRART-QRKHFRESRENVWKRPKPDPNSVNDNKDGNGNPWEPFESQSPAFNEYYK 62
           G+GR  R  T +R     S ++  ++P     S + N     N  +P  S +  F EYYK
Sbjct: 2   GRGRRHRGSTSKRNQIENSNDDNVRKPLKSEASFDSN-----NLQKPICS-NLHFEEYYK 61

Query: 63  GQGVVSAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLQAEVADGDEAE 122
            Q +V A+EWD+F+E LRKPLPAAFR+ S+GQFC EIR +L+NDFM SLQAE  +  E E
Sbjct: 62  EQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESGELE 121

Query: 123 AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLD 182
           AI+PLPWYP NLAWHSNFSR ++RKNQ LERFHEFLKLE E GN+TRQE+VSMVPPLFLD
Sbjct: 122 AIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPLFLD 181

Query: 183 VHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCNLLIHQTKRMC 242
           VHP+H+VLDMCAAPGSKTFQLLEIIH+SS+PG LP+G+VVAND+D +R NLLIHQTKR C
Sbjct: 182 VHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTKRTC 241

Query: 243 TANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHN-TQLTFDRVLCDVPCSGDGTLRK 302
           T NL+VTN+E QHFP C    N     +  +E  PH   QL FDRVLCDVPCSGDGTLRK
Sbjct: 242 TTNLMVTNNEGQHFPSC----NTKRTLSVASETNPHPIDQLLFDRVLCDVPCSGDGTLRK 301

Query: 303 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 362
           APDIWR+WNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+LR+ 
Sbjct: 302 APDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRRC 361

Query: 363 GGSLELVDVSNELPQLVCRPGLKKWRVRDKGVWLASYKQVTECRQSVAIPSMFPSGRGRK 422
           G S+ELVDVS++LP+L+ RPGL KW+V D+G W  SYK V + ++   + SMFPSG+  K
Sbjct: 362 GCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKSDK 421

Query: 423 NQADDNHNSELGANHLDDSKGSSVDVSEPTTVPVHEFDEEVCEFPIERCMRIVPHDQNSG 482
                            DS G      E  ++   E  EEVC+ P+E CMRI+PHDQN+G
Sbjct: 422 -----------------DSSGGKNSYEEMASISSDESAEEVCDLPLELCMRILPHDQNTG 481

Query: 483 AFFIAVLRKVAPLPAATEKPSNQNSKSANDRSRCKLPQPTTDAECVDNATDDMDESPSEI 542
            FFIAVL KV+PLP   EK + + + S  +                              
Sbjct: 482 GFFIAVLHKVSPLPDFQEKVTQKRNLSTRN------------------------------ 541

Query: 543 EIDSNNQGDSSSADNKPACKLPVQDINAADGLDSNAADGIDKQLSEADLEVNLVDNEVSE 602
            I+S+ +  S  A               +D + +   +G ++ + EA          VSE
Sbjct: 542 NINSSEKSSSYEA--------------VSDTVVTKPEEGTEEIVLEA---------TVSE 601

Query: 603 NNLEITAIKDYSEESKQPGDGEMDPKKNTVKRKLQI---QGKWRGVDPVVLFKDEAVIDS 662
           N  E   +    E +   G  E+  K + ++ K ++   QGKW+G+ PVV  +D+  I+ 
Sbjct: 602 NGFEPPEM----ESTWNEGIVELAQKLDDIEGKREVPSMQGKWKGLYPVVFLRDQTGING 661

Query: 663 IKTFYGI-NESFPLIGHLVTRNSDTN---HVKRIYYISKSVKDVLELNFSVGQQLKITSI 722
           IKTFYGI +ESFPL GHLVTRNSD +   +VKRIYY+SK+VK+VLELNF+VG+ LKI+S+
Sbjct: 662 IKTFYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSV 721

Query: 723 GLKMFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYADF 782
           GLKMFE+QS++E  +  CSFRI+SEGLPVILPY+TKQIL  +  DFK+LLQ+KSIK+ DF
Sbjct: 722 GLKMFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDF 766

Query: 783 VDAAFGEKASNLMLGCCVIVLGTGSNTSSDIIKVDESTIAIGCWKGRASLSVMVTAVDCQ 842
           +    GEKA+ L LG CV+VL   S   S+  KV+ STIAIGCWKG+ASL+VMVT VDC 
Sbjct: 782 IHPQIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCH 766

BLAST of Spg012808.1 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 2.9e-16
Identity = 80/304 (26.32%), Postives = 126/304 (41.45%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQ 228
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN++ V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 229 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLC 288
           R   L     RM   N IV N++                   G EL     Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYD-------------------GRELPKVLGQNTVDRVLL 463

Query: 289 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 523

Query: 349 NPVENEAVVAELLRKSGGSLELVDVS------NELPQLVCRPGLKKWR------VRDKGV 408
              ENEAV+   L+K    L    +           +   +P L K R          G 
Sbjct: 524 MVTENEAVIDYALKKRDVKLVTCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGF 583

Query: 409 WLASYKQVTECRQSVAIPSMFPSGRGRKNQADDNHNSELGANHLDDSKGSSVDVSEPTTV 457
           ++A  K+++  +QS        S  G  +  +    +E+ ++  DD +  +++ +E  +V
Sbjct: 584 FVAKLKKMSNVKQS--------SEEGDDDAVETVEQAEVSSD--DDDEAEAIEETEKPSV 610

BLAST of Spg012808.1 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 5.5e-15
Identity = 60/194 (30.93%), Postives = 85/194 (43.81%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQ 228
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN++ V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 229 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLC 288
           R   L     RM   N IV N++                   G EL     Q + DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYD-------------------GRELTKVLGQSSVDRVLL 446

Query: 289 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 493

Query: 349 NPVENEAVVAELLR 359
              ENEAV+   L+
Sbjct: 507 MIPENEAVIDYALK 493

BLAST of Spg012808.1 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 76.6 bits (187), Expect = 1.0e-13
Identity = 63/190 (33.16%), Postives = 91/190 (47.89%), Query Frame = 0

Query: 172 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPHGLVVANDLDVQRCN 231
           VS+V P      P   ++D CAAPG KT  +   +           G++ A D++  R  
Sbjct: 323 VSVVKP-----QPGERIMDACAAPGGKTLFMASCLK--------GQGMIYAMDVNEGRLR 382

Query: 232 LLIHQTKRMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPHNTQLTFDRVLCDVP 291
           +L    K      LI T H         +++  F   A  NE++       +D+VL D P
Sbjct: 383 ILGETAKSHQVDGLITTIH---------SDLRVF---AETNEVQ-------YDKVLLDAP 442

Query: 292 CSGDGTLRKAPDIWRKWNSGMGNGLH--GLQVQIGMRGASLLKVGGRMVYSTCSMNPVEN 351
           CSG G L K  D+  +WN  + + L    LQ ++    + L+K GG +VYSTCS++P EN
Sbjct: 443 CSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKHGGVLVYSTCSIDPEEN 478

Query: 352 EA-VVAELLR 359
           E  V A LLR
Sbjct: 503 EGRVEAFLLR 478