Spg012035.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg012035.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Vacuolar ATPase assembly integral membrane protein VMA21 homolog
Location	scaffold1: 667045 .. 668963 (+)
Sequence length	255
RNA-Seq Expression	Spg012035.1
Synteny	Spg012035.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATGCCCATTTTCCCAAAAGCACCCTCTGTAATTCACGCTCCCTACCACCATTAAAAGCATACGCCAGATGAAACTGACTTTCAGAATTGGACTTCCCATTTATACCCATCTTTCTCTATTTTCGATTGGAAGCCTAACACTATCTTACCTCATTCATATAAAGAACCCAGTCTTTTCTAGGTTTTTTCCTTTTTCCCAATGTTCTGATACGTGCCAATAAGTGCAATAAGTGCATTTTGGTCTTGTCTGGATGGTGTTACTTAAAGCGCATGGTTTAGTATGCCATCTTTATATTTTCATGGCTACTTGTCCTAGAACTCAACAATTGGTTGATGATACATAGTTCTATGGAAGTTTAATTGCTCGCTCTGGACCCTGTATTCTTCCTATCTGTGAATTTGTTAGTATAGCAACAACTTTTATGTCAAATGTTAAAATATGCACGAGGAATGTGGAAACCTTGAACTTCCTCTTTTTCCTTCCTCAACAAACATATATAAAGCTCTCTCGATATTAGTTGCCCCCCTTCCTTACTGCACTCTTTACTTATAACTGTTGGTCATTAACAAATCACTCTTACTCTACCTCTGATGGGGTACTATTAAGATGACTCTTTCTATTAAGCAAAGATATTTCTTCATATTTGTTGTTATGGATATTGTATTATTATTATTATTATTATTTATTGGCTTGTTGTAGCTTGGCATTAACTTGTTTTTTCTTCTCCTCTTCTTCCCTTTTATTTTCCTGCGTGTGATTACATTTCTAAGTAAATGCTTCTTGAGCAGGTTCAACACAATTAAATCCACATTCTCTTACACTCTTGAGTGGATTTCTTGCCGTCATATCAGTCAACGTTGTCATTGCTCTTTATATTATAATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTCTTGCTGAGGCAAAAGCTAGTATAAACCAGTCTACTGGGAAAGCAGATGACCCCTCGCAAGCTAATAATAAGAAGGAAGAATAGGAAATGAGAGAGACAGAGGGAGAGGATCTGATGAGGAACACTTTCAAGCTCAATCTTCCAGGATTTGAGATGAGAATCAGTCTCATGTGTGGACGCTTGTCAGTTGAGACATAGGAAGAACAAAAGGTTCCGTTTCTTCTCTAGTTTGTTTCATTAAAATAGACTGCATTTAGATTTGTACTGCTGATGTTCTTCACTGTGTTGTCAACTCTTTGCTTCTACTGTCATAACGTGAAGAGATGTAATAGCTTTTGGGGAGAATTAAAAAAAATTTTAGATTCTTGCATTTCTGGCTTCATGTTTCAAAATTTAACATAATCCTGATGGTTTTAATCTCTTGTGTTAACAAAGCTTGGCCTTAGTCCAAGGAAGGACCATTTATGTACTCATACTTACAAAAAGAACATGACCTTTGAACCTCTCTTGAGGTAAACTTTATCTCGCTTTCATGCAGTTTGAATTGATGAGATAAATTACATTTTAGTAACTCTAATTTATCTCGCTTTTACTGCGTTCTTCTTATATTCATTGCCAGGTCATAGCACATTTTTCTCTCCATAGATGATTTCTAGATTGTTCTTCATCACCAACCATCTTCAAATGTTTCCAATTCATTTTATTGTGAGTTTGGGTTTTAGCTAGTTTTCCAGCGAGTAAATTTTTGGATGACCATTTGTGCAACATAAACTTTTGGCTATTTTTCAATTTAAATGTGTAAAGATAGACATGTAAACTAATTTTTCTTTTGAATTTGGGCTGAGTTAATACAGTTTTACTATTATTTTTGGGCAAGACTACTCTATGTGCAACCACCTTTTCTTTTTCTAATTACTCTAATTTCTTCTTCAATGTCTTTTTACTCGTATCATATCATCTACTAATATATATTTTTTACATAAAAAT

mRNA sequence

ATGTATGCCCATTTTCCCAAAAGCACCCTCTGTTCAACACAATTAAATCCACATTCTCTTACACTCTTGAGTGGATTTCTTGCCGTCATATCAGTCAACGTTGTCATTGCTCTTTATATTATAATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTCTTGCTGAGGCAAAAGCTAGTATAAACCAGTCTACTGGGAAAGCAGATGACCCCTCGCAAGCTAATAATAAGAAGGAAGAATAG

Coding sequence (CDS)

ATGTATGCCCATTTTCCCAAAAGCACCCTCTGTTCAACACAATTAAATCCACATTCTCTTACACTCTTGAGTGGATTTCTTGCCGTCATATCAGTCAACGTTGTCATTGCTCTTTATATTATAATGGCAATGAAAGAACCCTCTAGTAAACATGAACCGGATCCTGCATTTCTTGCTGAGGCAAAAGCTAGTATAAACCAGTCTACTGGGAAAGCAGATGACCCCTCGCAAGCTAATAATAAGAAGGAAGAATAG

Protein sequence

MYAHFPKSTLCSTQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGKADDPSQANNKKEE

Homology

[Close]

BLAST of Spg012035.1 vs. NCBI nr
Match: XP_022926009.1 (uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_022926010.1 uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_023543189.1 uncharacterized protein LOC111803138 [Cucurbita pepo subsp. pepo] >KAG7034797.1 hypothetical protein SDJN02_04529 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 129.8 bits (325), Expect = 1.1e-26
Identity = 69/73 (94.52%), Postives = 72/73 (98.63%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           STQLNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDPAF+AEAKASINQS GK
Sbjct: 34  STQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPGK 93

Query: 72  ADDPSQANNKKEE 85
           A+DPSQANNKKEE
Sbjct: 94  AEDPSQANNKKEE 106

[Close]

BLAST of Spg012035.1 vs. NCBI nr
Match: KAG6581507.1 (hypothetical protein SDJN03_21509, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.8 bits (325), Expect = 1.1e-26
Identity = 70/75 (93.33%), Postives = 73/75 (97.33%), Query Frame = 0


Query: 10  LCSTQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQST 69
           L STQLNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDPAF+AEAKASINQS 
Sbjct: 109 LGSTQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSP 168

Query: 70  GKADDPSQANNKKEE 85
           GKA+DPSQANNKKEE
Sbjct: 169 GKAEDPSQANNKKEE 183

[Close]

BLAST of Spg012035.1 vs. NCBI nr
Match: XP_004134542.1 (uncharacterized protein LOC101206493 [Cucumis sativus] >XP_011658340.1 uncharacterized protein LOC101206493 [Cucumis sativus] >KAE8647778.1 hypothetical protein Csa_003577 [Cucumis sativus])

HSP 1 Score: 128.3 bits (321), Expect = 3.2e-26
Identity = 69/73 (94.52%), Postives = 70/73 (95.89%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           STQLNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDP FLAEAKASINQSTGK
Sbjct: 34  STQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPTFLAEAKASINQSTGK 93

Query: 72  ADDPSQANNKKEE 85
           AD PSQA NKKEE
Sbjct: 94  ADGPSQAQNKKEE 106

[Close]

BLAST of Spg012035.1 vs. NCBI nr
Match: XP_022977993.1 (uncharacterized protein LOC111478118 [Cucurbita maxima] >XP_022977994.1 uncharacterized protein LOC111478118 [Cucurbita maxima])

HSP 1 Score: 126.7 bits (317), Expect = 9.3e-26
Identity = 67/73 (91.78%), Postives = 71/73 (97.26%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST LNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDPAF+AEAKASINQ+ GK
Sbjct: 34  STHLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQAPGK 93

Query: 72  ADDPSQANNKKEE 85
           A+DPSQANNKKEE
Sbjct: 94  AEDPSQANNKKEE 106

[Close]

BLAST of Spg012035.1 vs. NCBI nr
Match: KAA0052446.1 (hypothetical protein E6C27_scaffold120G00430 [Cucumis melo var. makuwa] >TYK13376.1 hypothetical protein E5676_scaffold255G0010040 [Cucumis melo var. makuwa])

HSP 1 Score: 125.9 bits (315), Expect = 1.6e-25
Identity = 67/73 (91.78%), Postives = 71/73 (97.26%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST+LNPHSLTLLSGFLAVISVNVVI +YIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK
Sbjct: 118 STRLNPHSLTLLSGFLAVISVNVVIVIYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 177

Query: 72  ADDPSQANNKKEE 85
           AD PS+A+NKKEE
Sbjct: 178 ADGPSEAHNKKEE 190

[Close]

BLAST of Spg012035.1 vs. ExPASy TrEMBL
Match: A0A6J1EJT7 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita moschata OX=3662 GN=LOC111433255 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.3e-27
Identity = 69/73 (94.52%), Postives = 72/73 (98.63%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           STQLNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDPAF+AEAKASINQS GK
Sbjct: 34  STQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQSPGK 93

Query: 72  ADDPSQANNKKEE 85
           A+DPSQANNKKEE
Sbjct: 94  AEDPSQANNKKEE 106

[Close]

BLAST of Spg012035.1 vs. ExPASy TrEMBL
Match: A0A0A0KPB7 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis sativus OX=3659 GN=Csa_6G526400 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-26
Identity = 69/73 (94.52%), Postives = 70/73 (95.89%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           STQLNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDP FLAEAKASINQSTGK
Sbjct: 206 STQLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPTFLAEAKASINQSTGK 265

Query: 72  ADDPSQANNKKEE 85
           AD PSQA NKKEE
Sbjct: 266 ADGPSQAQNKKEE 278

[Close]

BLAST of Spg012035.1 vs. ExPASy TrEMBL
Match: A0A6J1ISV9 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita maxima OX=3661 GN=LOC111478118 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-26
Identity = 67/73 (91.78%), Postives = 71/73 (97.26%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST LNPHSLTLLSGFLAVISVNVVIA+YIIMAMKEPSSKHEPDPAF+AEAKASINQ+ GK
Sbjct: 34  STHLNPHSLTLLSGFLAVISVNVVIAIYIIMAMKEPSSKHEPDPAFVAEAKASINQAPGK 93

Query: 72  ADDPSQANNKKEE 85
           A+DPSQANNKKEE
Sbjct: 94  AEDPSQANNKKEE 106

[Close]

BLAST of Spg012035.1 vs. ExPASy TrEMBL
Match: A0A1S4DTT6 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo OX=3656 GN=LOC103484273 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.7e-26
Identity = 67/73 (91.78%), Postives = 71/73 (97.26%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST+LNPHSLTLLSGFLAVISVNVVI +YIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK
Sbjct: 34  STRLNPHSLTLLSGFLAVISVNVVIVIYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 93

Query: 72  ADDPSQANNKKEE 85
           AD PS+A+NKKEE
Sbjct: 94  ADGPSEAHNKKEE 106

[Close]

BLAST of Spg012035.1 vs. ExPASy TrEMBL
Match: A0A5A7UE48 (Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G0010040 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.7e-26
Identity = 67/73 (91.78%), Postives = 71/73 (97.26%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST+LNPHSLTLLSGFLAVISVNVVI +YIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK
Sbjct: 118 STRLNPHSLTLLSGFLAVISVNVVIVIYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 177

Query: 72  ADDPSQANNKKEE 85
           AD PS+A+NKKEE
Sbjct: 178 ADGPSEAHNKKEE 190

[Close]

BLAST of Spg012035.1 vs. TAIR 10
Match: AT1G05780.1 (Vacuolar ATPase assembly integral membrane protein VMA21-like domain )

HSP 1 Score: 80.9 bits (198), Expect = 5.5e-16
Identity = 46/73 (63.01%), Postives = 53/73 (72.60%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST L+PHSL LLSGFLAV+SVN VI  YI MAMKEP+ KH+PD AFLAEAK S+N+ T  
Sbjct: 34  STTLSPHSLILLSGFLAVVSVNTVIVFYICMAMKEPADKHKPDAAFLAEAKDSVNKLTKG 93

Query: 72  ADDPSQANNKKEE 85
           A        KK+E
Sbjct: 94  ATSSDDHALKKQE 106

[Close]

BLAST of Spg012035.1 vs. TAIR 10
Match: AT2G31710.1 (Vacuolar ATPase assembly integral membrane protein VMA21-like domain )

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-15
Identity = 42/72 (58.33%), Postives = 54/72 (75.00%), Query Frame = 0


Query: 12  STQLNPHSLTLLSGFLAVISVNVVIALYIIMAMKEPSSKHEPDPAFLAEAKASINQSTGK 71
           ST L+PHSLTLLSGFLAV+SVNVVI  YI +A+KEP+ KH+PD +F+AEAK S+ + T  
Sbjct: 34  STTLSPHSLTLLSGFLAVVSVNVVIVFYICLALKEPADKHKPDASFVAEAKDSVKKLTSG 93

Query: 72  ADDPSQANNKKE 84
             +   A  K+E
Sbjct: 94  VTNTDPALKKQE 105

The following BLAST results are available for this feature:

BLAST of Spg012035.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926009.1	1.1e-26	94.52	uncharacterized protein LOC111433255 [Cucurbita moschata] >XP_022926010.1 unchar...	[more]
KAG6581507.1	1.1e-26	93.33	hypothetical protein SDJN03_21509, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004134542.1	3.2e-26	94.52	uncharacterized protein LOC101206493 [Cucumis sativus] >XP_011658340.1 uncharact...	[more]
XP_022977993.1	9.3e-26	91.78	uncharacterized protein LOC111478118 [Cucurbita maxima] >XP_022977994.1 uncharac...	[more]
KAA0052446.1	1.6e-25	91.78	hypothetical protein E6C27_scaffold120G00430 [Cucumis melo var. makuwa] >TYK1337...	[more]

BLAST of Spg012035.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg012035.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJT7	5.3e-27	94.52	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita mo...	[more]
A0A0A0KPB7	1.6e-26	94.52	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis sati...	[more]
A0A6J1ISV9	4.5e-26	91.78	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucurbita ma...	[more]
A0A1S4DTT6	7.7e-26	91.78	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo...	[more]
A0A5A7UE48	7.7e-26	91.78	Vacuolar ATPase assembly integral membrane protein VMA21 homolog OS=Cucumis melo...	[more]

BLAST of Spg012035.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05780.1	5.5e-16	63.01	Vacuolar ATPase assembly integral membrane protein VMA21-like domain	[more]
AT2G31710.1	3.5e-15	58.33	Vacuolar ATPase assembly integral membrane protein VMA21-like domain	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019013	Vacuolar ATPase assembly integral membrane protein Vma21	PFAM	PF09446	VMA21	coord: 12..44 e-value: 5.0E-10 score: 39.4
IPR019013	Vacuolar ATPase assembly integral membrane protein Vma21	PANTHER	PTHR31792	VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21	coord: 11..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..84
None	No IPR available	PANTHER	PTHR31792:SF3	VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21	coord: 11..83

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg012035	Spg012035	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg012035.1-cds	Spg012035.1-cds-scaffold1:667045..667075	CDS
Spg012035.1-cds	Spg012035.1-cds-scaffold1:667837..668060	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg012035.1-three_prime_utr	Spg012035.1-three_prime_utr-scaffold1:668061..668963	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg012035.1	Spg012035.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070072	vacuolar proton-transporting V-type ATPase complex assembly
cellular_component	GO:0033116	endoplasmic reticulum-Golgi intermediate compartment membrane
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0012507	ER to Golgi transport vesicle membrane
cellular_component	GO:0016021	integral component of membrane