Spg010992.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg010992.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA/RNA polymerases superfamily protein
Location	scaffold4: 26727094 .. 26728787 (-)
Sequence length	531
RNA-Seq Expression	Spg010992.1
Synteny	Spg010992.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGGTGAGGTTTGATTTTCTTGAAAGTTAGTTCATTTTCTAACAAATTTCATGAAGGAAGCTTGACCTAAATCGTATGAGAAGTTGATGGGTTTTGGAATGGAAAACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGATGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGGTATGGTTGGAAAGCTTATTCAATTATCTACAACTTTCATGAAGAATTCGTGAGCCAAAAATGACTTTAATTAGGTTTAATTTCAGGAGGAAGTTAAAGGGTAACAGAGAAGCTCGAATCTGTGTTGTCTGACTTCGGGGCATTTCTGGACACATTGGTTGGGAATCTCATTTTCATTTCTTCAGGTACGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGTATTTCATTAATTTTGGTTCAGTATTGAGGAAGTTATGGGTATTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATTGTGAATGTGATTCTTAATCCAATTGTTTAAAAATTCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCGGTTTTGGTTATTTGACAGGCCAAGAGGAAATAGGTTGAGTCTACAAACCGGGGAACTAGATAAGACTGTGAGTGACCAAAATGAATTTCGAAAATGTTCTTAAACGGTTGTTCATGATTATATGTTTTATATGCTTTACCATGAATGATATATGATTTTTATGAAAGGTTTCAAGCATGAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGTCTAATGACTTTATGAAAAATAGCTGAAACGATATCTTTTAAAGTAAAGTATGGATTAGCAAGTACTTTTAAACGATTTCCAGTTTTTCATGAGCAAGCTGAGTAAGCTTAAGTGTTACGAAAGATAACGATAAGCAGACATGTTTTAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCTCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTACTTGATATGCCTTGCTAGATTTTTAATGACGAACTTGCGAGATATTTTAGAAAGCCTTTTTCATTACACGTTTATGTTAAACGCTTTGATTGAGCATATTTATTCGTTTAAAGGTTTATCACGTGTTTGACTTCTGGATTTAGAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATCCTTTCAAAATGT

mRNA sequence

ATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGGTACGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGCCAAGAGGAAATAGGTTGAGTCTACAAACCGGGGAACTAGATAAGACTCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCTCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAG

Coding sequence (CDS)

ATGGACAGCACAAAGCCCACCACCGACAACCACATTTTTCAGCCCAATTTCATTTCATTTCTCCATCATTTTCCATTCAAACTTTCAGAGCACTTTGAGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGAAAAAGTTCATGCAAAGCCAGCCATTGCAGAATCTGGGTGCAAACTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACAGAAGGAATCAAGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGGTACGTTGTAGAGTGTTCGAAAATGAAGAGTTTGATGTGCCAAGAGGAAATAGGTTGAGTCTACAAACCGGGGAACTAGATAAGACTCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCTCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAG

Protein sequence

MDSTKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDVPRGNRLSLQTGELDKTPEAIWYRVHTGYIPLLTLSVLRDNDAVVSAGRASLRQRRWECWAGPTTS

Homology

[Close]

BLAST of Spg010992.1 vs. NCBI nr
Match: TYK08801.1 (hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa])

HSP 1 Score: 141.7 bits (356), Expect = 5.9e-30
Identity = 92/164 (56.10%), Postives = 104/164 (63.41%), Query Frame = 0


Query: 12  IFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNL 71
           +FQPNFISFLHHFP KLSEHFEERVK EEEEEFRGLRLKKIGKSSCKA+HCRIW    + 
Sbjct: 56  LFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCRIW----ST 115

Query: 72  WFFIR---FELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDV-PRGNRLSLQTGELDKT 131
            F  +   F +         V K NQKLY       ++ ++DV PRGNR SLQTG+LDK 
Sbjct: 116 GFSSKPRGFLVFSDALSILEVQKSNQKLYW-DIESGKSGKWDVMPRGNRSSLQTGQLDKI 175

Query: 132 PEAIWYRVH------------TGYIPLLTLSVLRDNDAVVSAGR 160
             +I Y                   PL TLSVLRDNDAVVS G+
Sbjct: 176 EISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDNDAVVSVGQ 213

[Close]

BLAST of Spg010992.1 vs. NCBI nr
Match: TYK04298.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 104.4 bits (259), Expect = 1.0e-18
Identity = 79/153 (51.63%), Postives = 92/153 (60.13%), Query Frame = 0


Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTT   IFQ NFISFLHHFP KL EHFEERVK EEEEEFR ++ +   +S+  +S   
Sbjct: 15  TKPTTYKLIFQRNFISFLHHFPSKLLEHFEERVK-EEEEEFRAIQKELRDESTGFSS--- 74

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDV-PRGNRLSLQTG 123
                     F+ F      QE   V+K NQKLYL      ++ ++DV PRGNR SLQTG
Sbjct: 75  ------KPGGFLVFSDALSIQE---VHKSNQKLYL-DIESGKSGKWDVMPRGNRSSLQTG 134

Query: 124 ELDKTPEAIWYRVHTGYIPLLTLSVLRDNDAVV 156
           ELDKT   +          L TLSVLRDNDAVV
Sbjct: 135 ELDKTVIFL----------LSTLSVLRDNDAVV 143

[Close]

BLAST of Spg010992.1 vs. NCBI nr
Match: KAA0040575.1 (putative integrase [Cucumis melo var. makuwa] >TYK05618.1 putative integrase [Cucumis melo var. makuwa])

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-18
Identity = 69/134 (51.49%), Postives = 82/134 (61.19%), Query Frame = 0


Query: 30  EHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRF-------ELHRR 89
           +HFEERVK EEEEEFRGLRLKKIGKSSC ASHCRIWVQ      F+ F       E+H+ 
Sbjct: 53  KHFEERVK-EEEEEFRGLRLKKIGKSSCNASHCRIWVQLSTSGGFLVFSDALSILEVHKS 112

Query: 90  NQEHSGVYKLNQKL-YLVRCRVFENEEFDVPRGNRLSLQTGELDKTPEAIWYRVHTGYIP 149
           NQ+   +  L     +L+     ++ ++D+ RGNR SLQ GELDKT             P
Sbjct: 113 NQKLCLMNWLRISFSFLLDIESGKSGKWDL-RGNRPSLQIGELDKTV----------IFP 172

Query: 150 LLTLSVLRDNDAVV 156
           L T  VLRDNDAVV
Sbjct: 173 LSTFCVLRDNDAVV 174

[Close]

BLAST of Spg010992.1 vs. NCBI nr
Match: KAA0053276.1 (uncharacterized protein E6C27_scaffold102G00750 [Cucumis melo var. makuwa])

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-18
Identity = 73/152 (48.03%), Postives = 83/152 (54.61%), Query Frame = 0


Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTTD  IFQ NFI FLHHF  KLSE+F+ERVK EEEEEFRGLRLKK+ K   ++   +
Sbjct: 145 TKPTTDKLIFQSNFILFLHHFSSKLSEYFKERVK-EEEEEFRGLRLKKM-KKFMQSQTLQ 204

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDVPRGNRLSLQTGE 123
               T+NLWF                         V   +   E    PRGNR SLQTG+
Sbjct: 205 NLGATINLWF-------------------------VLIGLSYPEGTMRPRGNRPSLQTGK 259

Query: 124 LDKTPEAIWYRVHTGYIPLLTLSVLRDNDAVV 156
           LDKT              L TLSVLRDNDAV+
Sbjct: 265 LDKTV----------IFQLSTLSVLRDNDAVL 259

[Close]

BLAST of Spg010992.1 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 90.5 bits (223), Expect = 1.6e-14
Identity = 47/82 (57.32%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 95  KLYLVRCRVFENEEFDVPRGNRLSLQTGELDKTPEAIWYRVHTGYIPLLTLSVLRDNDAV 154
           +L+++  R+   E  ++PRGNRLSLQT E+DKT             PL TLSVL DNDAV
Sbjct: 66  QLWVLEVRISNLENLEMPRGNRLSLQTEEIDKTV----------IFPLSTLSVLSDNDAV 125

Query: 155 VSAGRASLRQRRWECWAGPTTS 177
           VSAG+A LRQRRWEC  GPTTS
Sbjct: 126 VSAGQAPLRQRRWECRTGPTTS 137

[Close]

BLAST of Spg010992.1 vs. ExPASy TrEMBL
Match: A0A5D3CAC6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00040 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 2.8e-30
Identity = 92/164 (56.10%), Postives = 104/164 (63.41%), Query Frame = 0


Query: 12  IFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNL 71
           +FQPNFISFLHHFP KLSEHFEERVK EEEEEFRGLRLKKIGKSSCKA+HCRIW    + 
Sbjct: 56  LFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCRIW----ST 115

Query: 72  WFFIR---FELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDV-PRGNRLSLQTGELDKT 131
            F  +   F +         V K NQKLY       ++ ++DV PRGNR SLQTG+LDK 
Sbjct: 116 GFSSKPRGFLVFSDALSILEVQKSNQKLYW-DIESGKSGKWDVMPRGNRSSLQTGQLDKI 175

Query: 132 PEAIWYRVH------------TGYIPLLTLSVLRDNDAVVSAGR 160
             +I Y                   PL TLSVLRDNDAVVS G+
Sbjct: 176 EISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDNDAVVSVGQ 213

[Close]

BLAST of Spg010992.1 vs. ExPASy TrEMBL
Match: A0A5D3BZ50 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00270 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 5.0e-19
Identity = 79/153 (51.63%), Postives = 92/153 (60.13%), Query Frame = 0


Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTT   IFQ NFISFLHHFP KL EHFEERVK EEEEEFR ++ +   +S+  +S   
Sbjct: 15  TKPTTYKLIFQRNFISFLHHFPSKLLEHFEERVK-EEEEEFRAIQKELRDESTGFSS--- 74

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDV-PRGNRLSLQTG 123
                     F+ F      QE   V+K NQKLYL      ++ ++DV PRGNR SLQTG
Sbjct: 75  ------KPGGFLVFSDALSIQE---VHKSNQKLYL-DIESGKSGKWDVMPRGNRSSLQTG 134

Query: 124 ELDKTPEAIWYRVHTGYIPLLTLSVLRDNDAVV 156
           ELDKT   +          L TLSVLRDNDAVV
Sbjct: 135 ELDKTVIFL----------LSTLSVLRDNDAVV 143

[Close]

BLAST of Spg010992.1 vs. ExPASy TrEMBL
Match: A0A5A7TGM2 (Putative integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00750 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 69/134 (51.49%), Postives = 82/134 (61.19%), Query Frame = 0


Query: 30  EHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQTVNLWFFIRF-------ELHRR 89
           +HFEERVK EEEEEFRGLRLKKIGKSSC ASHCRIWVQ      F+ F       E+H+ 
Sbjct: 53  KHFEERVK-EEEEEFRGLRLKKIGKSSCNASHCRIWVQLSTSGGFLVFSDALSILEVHKS 112

Query: 90  NQEHSGVYKLNQKL-YLVRCRVFENEEFDVPRGNRLSLQTGELDKTPEAIWYRVHTGYIP 149
           NQ+   +  L     +L+     ++ ++D+ RGNR SLQ GELDKT             P
Sbjct: 113 NQKLCLMNWLRISFSFLLDIESGKSGKWDL-RGNRPSLQIGELDKTV----------IFP 172

Query: 150 LLTLSVLRDNDAVV 156
           L T  VLRDNDAVV
Sbjct: 173 LSTFCVLRDNDAVV 174

[Close]

BLAST of Spg010992.1 vs. ExPASy TrEMBL
Match: A0A5A7UGD0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold102G00750 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 73/152 (48.03%), Postives = 83/152 (54.61%), Query Frame = 0


Query: 4   TKPTTDNHIFQPNFISFLHHFPFKLSEHFEERVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           TKPTTD  IFQ NFI FLHHF  KLSE+F+ERVK EEEEEFRGLRLKK+ K   ++   +
Sbjct: 145 TKPTTDKLIFQSNFILFLHHFSSKLSEYFKERVK-EEEEEFRGLRLKKM-KKFMQSQTLQ 204

Query: 64  IWVQTVNLWFFIRFELHRRNQEHSGVYKLNQKLYLVRCRVFENEEFDVPRGNRLSLQTGE 123
               T+NLWF                         V   +   E    PRGNR SLQTG+
Sbjct: 205 NLGATINLWF-------------------------VLIGLSYPEGTMRPRGNRPSLQTGK 259

Query: 124 LDKTPEAIWYRVHTGYIPLLTLSVLRDNDAVV 156
           LDKT              L TLSVLRDNDAV+
Sbjct: 265 LDKTV----------IFQLSTLSVLRDNDAVL 259

[Close]

BLAST of Spg010992.1 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.5e-15
Identity = 47/82 (57.32%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 95  KLYLVRCRVFENEEFDVPRGNRLSLQTGELDKTPEAIWYRVHTGYIPLLTLSVLRDNDAV 154
           +L+++  R+   E  ++PRGNRLSLQT E+DKT             PL TLSVL DNDAV
Sbjct: 66  QLWVLEVRISNLENLEMPRGNRLSLQTEEIDKTV----------IFPLSTLSVLSDNDAV 125

Query: 155 VSAGRASLRQRRWECWAGPTTS 177
           VSAG+A LRQRRWEC  GPTTS
Sbjct: 126 VSAGQAPLRQRRWECRTGPTTS 137

The following BLAST results are available for this feature:

BLAST of Spg010992.1 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK08801.1	5.9e-30	56.10	hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa]	[more]
TYK04298.1	1.0e-18	51.63	pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa]	[more]
KAA0040575.1	2.3e-18	51.49	putative integrase [Cucumis melo var. makuwa] >TYK05618.1 putative integrase [Cu...	[more]
KAA0053276.1	2.3e-18	48.03	uncharacterized protein E6C27_scaffold102G00750 [Cucumis melo var. makuwa]	[more]
KAA0037581.1	1.6e-14	57.32	reverse transcriptase [Cucumis melo var. makuwa]	[more]

BLAST of Spg010992.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg010992.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CAC6	2.8e-30	56.10	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3BZ50	5.0e-19	51.63	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A5A7TGM2	1.1e-18	51.49	Putative integrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00...	[more]
A0A5A7UGD0	1.1e-18	48.03	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3BTP3	7.5e-15	57.32	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37...	[more]

BLAST of Spg010992.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg010992	Spg010992	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg010992.1-three_prime_utr	Spg010992.1-three_prime_utr-scaffold4:26727094..26727364	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg010992.1-cds	Spg010992.1-cds-scaffold4:26727365..26727514	CDS
Spg010992.1-cds	Spg010992.1-cds-scaffold4:26727876..26727924	CDS
Spg010992.1-cds	Spg010992.1-cds-scaffold4:26728109..26728146	CDS
Spg010992.1-cds	Spg010992.1-cds-scaffold4:26728332..26728370	CDS
Spg010992.1-cds	Spg010992.1-cds-scaffold4:26728533..26728787	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg010992.1	Spg010992.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016740	transferase activity