Spg009660.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg009660.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	thioredoxin-like 3-2, chloroplastic
Location	scaffold7: 5834104 .. 5836561 (+)
Sequence length	591
RNA-Seq Expression	Spg009660.1
Synteny	Spg009660.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCCCAATAATTTAGAAAATTTGAATGAAAAAAAAAAAAAAAAAAAGTCCAAGGAATGCATGCGGCAGCCAGCCACGACTTGCACTTCAATTTAATTTGTTTTTTTTCTAATTTTTAAATTTCTATTATTTTATTTTGTTTCTTTTTTTTAAAAAAAAGTATTTTATTTTGTTTCTAAATTCTGTTTGTTTTTGTTTCTTCTTCAAATGCCGTTTCGTTAAGGTTTTACAGTTTTGGAGATCAATTGGAACAATCCCTCAACTGACTCTGAATCTCCCGGTGGGACCCCACCGGAACCCTCTTGAAGCCGCCGCCTCCGCCGCCGCCGCCAGTGGTCCGATCAAACCCACCAACTGCGGCCCTCTGACCTCTTCTTCCTCTTCTCTCTCTTTTTCTTTCTTTTGCCGTTTCTCAATTCTTCTCACTTCCGAAATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGTGAAATCTTTCCCCAAATTCGACGCCCCTTTTGATCTTCCGGTGCCGAGGCTCGTCGTCTCTGGTCACAACGGGCTTCCTTTGTCCCGAAAATTTCCGCCGCTTTCCCAGAAAGTTTCTGCGACTGGTGAGAAGATTTCGGTGCTCGGAGCTTGGACCCAAGAGAGTCCCCTGCAGTTTCGTGATGAATCGCCTGTGTCGATCGAGCTCAAGTCCATTTGCAGCGAACTTGAGTTCGATAAGGCTATTGCGGATGCAGGGGAACGCAACGAGCTCGTAATTCTCGTATGGTATGTATGCGTCTCTTGTGGGCTTTTCTGGGAAATGTTGAGTTAATTATGGAAGTTTCTTTCAGATTGTTTCTCCTCTTATAAGATATAGAGAAAGAAATCTGACGATGCGAATGTATTTGAGTTTGACTGGATCTTCTTTTATCAAAAATTTATTCGTTTATCAGGAAAAAATGTCCTCAACTGCTAGATTTTGCCTTGGATTTCGCTGAAACAGAATAATTTTGTGATCTTTTTGAAGTGAATGATGTGATAGTTATTCTCCAGTTTTCATTACCAGCCTTTCAAGTGCATTTCTCTTTATTTTATCTTCTTTCGTTGAAAATCGAGTTCATTTGGATCTGTAGGATGGCTAACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCTGATTATTACCCCAGGTTAGCTCTTAGCCCATTTCTAACCAACTTAATGGTTAACTGTGTTATCATTCTATAAGTGCTGGATAATATTTAAAAGACCACTGTGCCAGTCCTTATTGAAGTTGTGTATGTGCTTGATCAATTGTAGATTGCAATTCTATTGCATTGATGTCAATATGGTGCCACACAAGCTAGTTGTTCGTGCCGGATTAGCTGTAAGTTCCTTTCAAAGCTTTTTGATGTTTCTTCCATTTTGTTTGTGTTGCCATAAACCTTTATAATGGTCTTCATCTTCTTTAGGAAGGACTTGAGATTCTGAAAAGTTATTCTTCTGTAAGGGAGCGGTTGTCTTTAGATATTGTACTTGAGGTTCTGTTTACAATGGAATTCATACTTGTGTTTCGTTACCTTTAGGAACATTTCCAATTTGCAGTTTGAAGATAGGAGACAGAGGACATGTTGAGCTTGTTTTACTTTGATGGATGAGTTGATTGAATAAACTGCATGATCTGTTTTATCTCTCATTTTAATTTGTGCCTTTGATTCAAGTCCTGATAGCTTCAATTTTTATTTTTTCTCATGTATCTCAACCGTTCGCCTTTGAATCTACCAGAAGATGCCAGCTATACAGGTGAGTTTCTTCACAATCAGATGTCTTAAAACTGGAAACCTTCCTCTCTACTAATGTCAAGATCTTGATTATGTTTGTTGCAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTTGTCAACGACGTTCAGAAAATGATTGAACGTGAGCTAACAGGCGAGTGACTTACCATTGATTCTTACCCCCATCCTTTAGTGGTATTAGAATTGATTTGACAGCAACTCATAGAAATTCCTTTCTGCTTATATTCATCAACATAAAAGAACAAGGCCTGGCTGGCCCTCTTGCCGAGTCTTTTTCATTGGAGAGATCATTTATTTGTTGTCATGGGGTGGATGATCTAACATGTATGTCCAATTCTTAACGATATATGTATTAATTGTCACATTATGAGGAAGTCTTATTTGTAAGGTTTACCAGCCTTTTTATACCATTCATTTACATTATATCAGATATGCAGATTCCCCTGTTTTGTGTTGGTAGATTAATTTAGGACATGTTTGGGAGTGATTTTGGCCAAAATCACTTTTACTCTAGGACATGACAAAAATGATTTTAGTCATTCCAAAATTACTCTAAAACATGTCCTTAATTGTTCAGGGAATCTATTGTGAATAGCATGTCTCTTTTTGAGCCTTC

mRNA sequence

ATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGTGAAATCTTTCCCCAAATTCGACGCCCCTTTTGATCTTCCGGTGCCGAGGCTCGTCGTCTCTGGTCACAACGGGCTTCCTTTGTCCCGAAAATTTCCGCCGCTTTCCCAGAAAGTTTCTGCGACTGGTGAGAAGATTTCGGTGCTCGGAGCTTGGACCCAAGAGAGTCCCCTGCAGTTTCGTGATGAATCGCCTGTGTCGATCGAGCTCAAGTCCATTTGCAGCGAACTTGAGTTCGATAAGGCTATTGCGGATGCAGGGGAACGCAACGAGCTCGTAATTCTCGTATGGATGGCTAACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCTGATTATTACCCCAGATTGCAATTCTATTGCATTGATGTCAATATGGTGCCACACAAGCTAGTTGTTCGTGCCGGATTAGCTAAGATGCCAGCTATACAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTTGTCAACGACGTTCAGAAAATGATTGAACGTGAGCTAACAGGCGAGTGA

Coding sequence (CDS)

ATGGTGAAAGCTTTGCCCCTTCACCTCGGAACCGTGAAATCTTTCCCCAAATTCGACGCCCCTTTTGATCTTCCGGTGCCGAGGCTCGTCGTCTCTGGTCACAACGGGCTTCCTTTGTCCCGAAAATTTCCGCCGCTTTCCCAGAAAGTTTCTGCGACTGGTGAGAAGATTTCGGTGCTCGGAGCTTGGACCCAAGAGAGTCCCCTGCAGTTTCGTGATGAATCGCCTGTGTCGATCGAGCTCAAGTCCATTTGCAGCGAACTTGAGTTCGATAAGGCTATTGCGGATGCAGGGGAACGCAACGAGCTCGTAATTCTCGTATGGATGGCTAACTGGTGCAGAAATTATATTTATTTAAAACCCCAATTGGAAAGATTGGCTGCTGATTATTACCCCAGATTGCAATTCTATTGCATTGATGTCAATATGGTGCCACACAAGCTAGTTGTTCGTGCCGGATTAGCTAAGATGCCAGCTATACAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGATTGGTCTCTACAAAGCATATTTAGTTGTCAACGACGTTCAGAAAATGATTGAACGTGAGCTAACAGGCGAGTGA

Protein sequence

MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIERELTGE

Homology

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BLAST of Spg009660.1 vs. NCBI nr
Match: XP_022944830.1 (thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 5.8e-95
Identity = 175/196 (89.29%), Postives = 180/196 (91.84%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GTVKSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTVKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQFRDESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFRDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. NCBI nr
Match: XP_038891538.1 (thioredoxin-like 3-2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 5.8e-95
Identity = 180/203 (88.67%), Postives = 184/203 (90.64%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVP------RLVVSGHNGLPLSRKFPPLSQKVSATG 60
           MVKALPLHLG VKSFPKFD  FDLP P      RL  SG N LPL RKFPPLSQKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDGSFDLPPPPQLPSARLAGSGFNRLPLCRKFPPLSQKVSATG 60

Query: 61  EK-ISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWC 120
           EK  S+LGAWTQ+S LQFRDESPVSIELKSICSELEFDKAIADA +RNELVILVWMANWC
Sbjct: 61  EKTTSLLGAWTQDSTLQFRDESPVSIELKSICSELEFDKAIADAEKRNELVILVWMANWC 120

Query: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVI 180
           RNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVI
Sbjct: 121 RNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVI 180

Query: 181 GLYKAYLVVNDVQKMIERELTGE 197
           GLYKAYLV+NDVQKMIERELTGE
Sbjct: 181 GLYKAYLVINDVQKMIERELTGE 203

[Close]

BLAST of Spg009660.1 vs. NCBI nr
Match: KAG7012912.1 (Thioredoxin-like 3-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.5 bits (916), Expect = 7.6e-95
Identity = 175/196 (89.29%), Postives = 180/196 (91.84%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GTVKSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTVKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQFRDESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFRDESPVSIELKSICSELEFDKAIMDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. NCBI nr
Match: XP_023541920.1 (thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.5 bits (911), Expect = 2.9e-94
Identity = 174/196 (88.78%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GTVKS PK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTVKSIPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQFRDESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFRDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. NCBI nr
Match: XP_022967023.1 (thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 353.6 bits (906), Expect = 1.1e-93
Identity = 173/196 (88.27%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GT+KSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTLKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQF DESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFCDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot
Match: Q8VZT6 (Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC2 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-42
Identity = 81/155 (52.26%), Postives = 112/155 (72.26%), Query Frame = 0


Query: 38  PLSRKFPPLSQKVSATGEKISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADA 97
           P + K+   + K+    E+I  +    Q+  LQ  D+SPVS+EL  IC E  FD+ + DA
Sbjct: 35  PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVELGPICGESHFDQVMEDA 94

Query: 98  GERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKM 157
            +  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +DVN VP++LV RAG+ KM
Sbjct: 95  QKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDVNAVPYRLVSRAGVTKM 154

Query: 158 PAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIERE 193
           P IQLW+DG+KQ EVIG +KA+ VVN+V++MIE +
Sbjct: 155 PTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

[Close]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot
Match: Q9FG36 (Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC1 PE=2 SV=3)

HSP 1 Score: 119.8 bits (299), Expect = 3.5e-26
Identity = 52/116 (44.83%), Postives = 84/116 (72.41%), Query Frame = 0


Query: 76  PVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQ 135
           P ++E++ I +  E D  ++ A + ++ +I+ WMA+WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 136 FYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIER 192
           FY +DVN VP  LV R  ++KMP IQLWK+ + ++EVIG +K +LV+ +V+++I +
Sbjct: 153 FYYVDVNKVPQTLVKRGNISKMPTIQLWKEDEMKEEVIGGHKGWLVIEEVRELINK 208

[Close]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot
Match: Q6Z7L3 (Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0774100 PE=3 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 3.3e-24
Identity = 52/113 (46.02%), Postives = 82/113 (72.57%), Query Frame = 0


Query: 79  IELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYC 138
           +E++ I S  + D A+  + E  + +I+ WMA+WCR  IYLKP+LE++A + +P ++FY 
Sbjct: 80  VEMEEIDSMEKLDDALRWSVENKQPIIIDWMASWCRKCIYLKPRLEKIAGE-FPGVRFYF 139

Query: 139 IDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIER 192
           +DVN VP  +V R  + KMP IQLWKDG+   EVIG +KA+LV+++V++MI++
Sbjct: 140 VDVNKVPQTVVKRGNITKMPTIQLWKDGEWAAEVIGGHKAWLVMDEVREMIQK 191

[Close]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot
Match: P80028 (Thioredoxin H-type OS=Chlamydomonas reinhardtii OX=3055 GN=TRXH PE=1 SV=3)

HSP 1 Score: 53.5 bits (127), Expect = 3.1e-06
Identity = 27/91 (29.67%), Postives = 49/91 (53.85%), Query Frame = 0


Query: 84  ICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNM 143
           I S+  +D  +A   E ++ +++ + A WC     + P  E L+ DY  ++ F  +DV+ 
Sbjct: 8   IDSKAAWDAQLAKGKEEHKPIVVDFTATWCGPCKMIAPLFETLSNDYAGKVIFLKVDVDA 67

Query: 144 VPHKLVVRAGLAKMPAIQLWKDGKKQDEVIG 175
           V   +   AG+  MP   ++KDG K D+++G
Sbjct: 68  VA-AVAEAAGITAMPTFHVYKDGVKADDLVG 97

[Close]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot
Match: P37395 (Thioredoxin OS=Cyanidium caldarium OX=2771 GN=trxA PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-05
Identity = 26/104 (25.00%), Postives = 55/104 (52.88%), Query Frame = 0


Query: 86  SELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNMVP 145
           ++  F+K + ++    +LV++ + A WC     + P ++ LA +Y  +++   I+ +  P
Sbjct: 8   TDFSFEKEVVNS---EKLVLVDFWAPWCGPCRMISPVIDELAQEYVEQVKIVKINTDENP 67

Query: 146 HKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMI 190
             +    G+  +P + L+KDGK+ D VIG      + N ++K +
Sbjct: 68  -SISAEYGIRSIPTLMLFKDGKRVDTVIGAVPKSTLTNALKKYL 107

[Close]

BLAST of Spg009660.1 vs. ExPASy TrEMBL
Match: A0A6J1FZ60 (thioredoxin-like 3-2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449247 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.8e-95
Identity = 175/196 (89.29%), Postives = 180/196 (91.84%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GTVKSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTVKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQFRDESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFRDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. ExPASy TrEMBL
Match: A0A6J1HTY1 (thioredoxin-like 3-2, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466549 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.3e-94
Identity = 173/196 (88.27%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GT+KSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTLKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQF DESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFCDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 196

[Close]

BLAST of Spg009660.1 vs. ExPASy TrEMBL
Match: A0A5D3CG32 (Thioredoxin-like 3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002530 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.6e-93
Identity = 176/202 (87.13%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLP------VPRLVVSGHNGLPLSRKFPPLSQKVSATG 60
           MVKALPLHLG VKSFPKFD+ FDLP        RL  SG N LPLSRKFPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCR 120
           EK S+LGAWTQ+S LQFRDESPVSIELKSI SELEFDKAIADA ERNELVILVWMANWCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVVNDVQKMIERELTGE 197
           LYKAYLVVNDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of Spg009660.1 vs. ExPASy TrEMBL
Match: A0A1S3BGQ4 (thioredoxin-like 3-2, chloroplastic isoform X8 OS=Cucumis melo OX=3656 GN=LOC103489448 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.6e-93
Identity = 176/202 (87.13%), Postives = 182/202 (90.10%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLP------VPRLVVSGHNGLPLSRKFPPLSQKVSATG 60
           MVKALPLHLG VKSFPKFD+ FDLP        RL  SG N LPLSRKFPP+ QKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCR 120
           EK S+LGAWTQ+S LQFRDESPVSIELKSI SELEFDKAIADA ERNELVILVWMANWCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIG 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEV+G
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVG 180

Query: 181 LYKAYLVVNDVQKMIERELTGE 197
           LYKAYLVVNDV+KMIERELT E
Sbjct: 181 LYKAYLVVNDVRKMIERELTDE 202

[Close]

BLAST of Spg009660.1 vs. ExPASy TrEMBL
Match: A0A6J1FZ53 (thioredoxin-like 3-2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449247 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.4e-93
Identity = 174/196 (88.78%), Postives = 179/196 (91.33%), Query Frame = 0


Query: 1   MVKALPLHLGTVKSFPKFDAPFDLPVPRLVVSGHNGLPLSRKFPPLSQKVSATGEKISVL 60
           MVK LP+H GTVKSFPK DA FDLP  +L VSG N LPLS+KFPPLS KVSATGEKISVL
Sbjct: 1   MVKVLPVHFGTVKSFPKLDARFDLPAAKLTVSGFNRLPLSQKFPPLSLKVSATGEKISVL 60

Query: 61  GAWTQESPLQFRDESPVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLK 120
           G WTQES LQFRDESPVSIELKSICSELEFDKAI DA +RNE VI+VWMANWCRNYIYLK
Sbjct: 61  GDWTQESSLQFRDESPVSIELKSICSELEFDKAITDAEKRNEPVIIVWMANWCRNYIYLK 120

Query: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYL 180
           PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLA MPAIQLWKD KKQDEVIGLYKAYL
Sbjct: 121 PQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLA-MPAIQLWKDSKKQDEVIGLYKAYL 180

Query: 181 VVNDVQKMIERELTGE 197
           VVNDVQKMIERELTGE
Sbjct: 181 VVNDVQKMIERELTGE 195

[Close]

BLAST of Spg009660.1 vs. TAIR 10
Match: AT5G04260.1 (WCRKC thioredoxin 2 )

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-43
Identity = 81/155 (52.26%), Postives = 112/155 (72.26%), Query Frame = 0


Query: 38  PLSRKFPPLSQKVSATGEKISVLGAWTQESPLQFRDESPVSIELKSICSELEFDKAIADA 97
           P + K+   + K+    E+I  +    Q+  LQ  D+SPVS+EL  IC E  FD+ + DA
Sbjct: 35  PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVELGPICGESHFDQVMEDA 94

Query: 98  GERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAKM 157
            +  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +DVN VP++LV RAG+ KM
Sbjct: 95  QKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVDVNAVPYRLVSRAGVTKM 154

Query: 158 PAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIERE 193
           P IQLW+DG+KQ EVIG +KA+ VVN+V++MIE +
Sbjct: 155 PTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

[Close]

BLAST of Spg009660.1 vs. TAIR 10
Match: AT5G06690.1 (WCRKC thioredoxin 1 )

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-27
Identity = 52/116 (44.83%), Postives = 84/116 (72.41%), Query Frame = 0


Query: 76  PVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQ 135
           P ++E++ I +  E D  ++ A + ++ +I+ WMA+WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 136 FYCIDVNMVPHKLVVRAGLAKMPAIQLWKDGKKQDEVIGLYKAYLVVNDVQKMIER 192
           FY +DVN VP  LV R  ++KMP IQLWK+ + ++EVIG +K +LV+ +V+++I +
Sbjct: 153 FYYVDVNKVPQTLVKRGNISKMPTIQLWKEDEMKEEVIGGHKGWLVIEEVRELINK 208

[Close]

BLAST of Spg009660.1 vs. TAIR 10
Match: AT5G06690.2 (WCRKC thioredoxin 1 )

HSP 1 Score: 80.9 bits (198), Expect = 1.3e-15
Identity = 35/80 (43.75%), Postives = 55/80 (68.75%), Query Frame = 0


Query: 76  PVSIELKSICSELEFDKAIADAGERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQ 135
           P ++E++ I +  E D  ++ A + ++ +I+ WMA+WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 136 FYCIDVNMVPHKLVVRAGLA 156
           FY +DVN VP  LV R  ++
Sbjct: 153 FYYVDVNKVPQTLVKRGNIS 172

The following BLAST results are available for this feature:

BLAST of Spg009660.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944830.1	5.8e-95	89.29	thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita moschata]	[more]
XP_038891538.1	5.8e-95	88.67	thioredoxin-like 3-2, chloroplastic isoform X1 [Benincasa hispida]	[more]
KAG7012912.1	7.6e-95	89.29	Thioredoxin-like 3-2, chloroplastic, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_023541920.1	2.9e-94	88.78	thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022967023.1	1.1e-93	88.27	thioredoxin-like 3-2, chloroplastic isoform X1 [Cucurbita maxima]	[more]

BLAST of Spg009660.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZT6	1.6e-42	52.26	Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC2 PE...	[more]
Q9FG36	3.5e-26	44.83	Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=WCRKC1 PE...	[more]
Q6Z7L3	3.3e-24	46.02	Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
P80028	3.1e-06	29.67	Thioredoxin H-type OS=Chlamydomonas reinhardtii OX=3055 GN=TRXH PE=1 SV=3	[more]
P37395	4.4e-05	25.00	Thioredoxin OS=Cyanidium caldarium OX=2771 GN=trxA PE=3 SV=1	[more]

BLAST of Spg009660.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ60	2.8e-95	89.29	thioredoxin-like 3-2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HTY1	5.3e-94	88.27	thioredoxin-like 3-2, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5D3CG32	2.6e-93	87.13	Thioredoxin-like 3-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475...	[more]
A0A1S3BGQ4	2.6e-93	87.13	thioredoxin-like 3-2, chloroplastic isoform X8 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1FZ53	3.4e-93	88.78	thioredoxin-like 3-2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Spg009660.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04260.1	1.1e-43	52.26	WCRKC thioredoxin 2	[more]
AT5G06690.1	2.5e-27	44.83	WCRKC thioredoxin 1	[more]
AT5G06690.2	1.3e-15	43.75	WCRKC thioredoxin 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 72..188 e-value: 6.3E-13 score: 50.4
None	No IPR available	PANTHER	PTHR47192:SF4	THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC	coord: 33..193
None	No IPR available	CDD	cd02947	TRX_family	coord: 89..190 e-value: 8.00219E-12 score: 57.1829
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 87..178 e-value: 1.6E-9 score: 37.7
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 37..194 score: 8.914598
IPR044253	Thioredoxin-like 3-1/3-2	PANTHER	PTHR47192	THIOREDOXIN-LIKE 3-2, CHLOROPLASTIC	coord: 33..193
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 82..190

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg009660	Spg009660	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg009660.1-five_prime_utr	Spg009660.1-five_prime_utr-scaffold7:5834104..5834537	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg009660.1-cds	Spg009660.1-cds-scaffold7:5834538..5834860	CDS
Spg009660.1-cds	Spg009660.1-cds-scaffold7:5835208..5835282	CDS
Spg009660.1-cds	Spg009660.1-cds-scaffold7:5835413..5835479	CDS
Spg009660.1-cds	Spg009660.1-cds-scaffold7:5835878..5835895	CDS
Spg009660.1-cds	Spg009660.1-cds-scaffold7:5835979..5836086	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg009660.1-three_prime_utr	Spg009660.1-three_prime_utr-scaffold7:5836087..5836561	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg009660.1	Spg009660.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009570	chloroplast stroma