Spg009653.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg009653.1 vs. NCBI nr
Match: XP_022139254.1 (probable membrane-associated kinase regulator 4 [Momordica charantia])

HSP 1 Score: 602.8 bits (1553), Expect = 1.9e-168
Identity = 313/350 (89.43%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYDPADDDYIDMEV SYTNFLCHSKSSP + REFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKS+SDYD RKD FEEFYSTPLTT+VNTPTATSTPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSESCRISRELNPDEYLLEY+ D +YLN ESSKKSWT+K KHSSLGLKLKASRAY
Sbjct: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTKSGCSDESCAAATKIADEG ASKA+ECSKK AKVEKRKP GQ PSSIAN NKEK
Sbjct: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT+N  RNRRSFSM IKRQP+IKPSSSASLH SS+S  +NSS YFLKRCGSVNSEIENS
Sbjct: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSS+SLAVS DQEIPELCRG
Sbjct: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350

BLAST of Spg009653.1 vs. NCBI nr
Match: XP_023527329.1 (probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.4 bits (1526), Expect = 2.5e-165
Identity = 304/350 (86.86%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+GSYTNFLC SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKSSSDYD+RKD FEEF+STPLTTS+NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEY+ D SYLN ESSKKSWTK+ K S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKRLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTK+GCSDESCA A KIADEG ASKANECSKK AKVEKRKPFG   SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT++SS NRRSFS+ IKRQPNIKP SSASL+P+S+SCPNNSSGY+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKP-SSASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of Spg009653.1 vs. NCBI nr
Match: KAG6601024.1 (putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031828.1 putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 591.7 bits (1524), Expect = 4.3e-165
Identity = 304/350 (86.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+GSYTNFLC SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKSSSDYD+RKD FEEF+STPLTTS+NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEY+ D SYLN ESSKKSWTKK K S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTK+GCSDESCA A KIADEG ASKANECSKK AKVEKRKPFG   SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT++SS NRRSFS+ IKRQPNIKP SSASL+P+ +SCPNNSSGY+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKP-SSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of Spg009653.1 vs. NCBI nr
Match: XP_022956992.1 (probable membrane-associated kinase regulator 4 [Cucurbita moschata])

HSP 1 Score: 589.7 bits (1519), Expect = 1.6e-164
Identity = 303/350 (86.57%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+GSYTNFLC SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKSSSDYD+RKD FEEF+STPLTTS+NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEY+ D SYLN ESSKKSWTKK K S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTK+GCSDESCA A KIADEG ASKANECSKK AKVEKRKPFG   SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT++S  NRRSFS+ IKRQPNIKP SSASL+P+ +SCPNNSSGY+LKRCGSVNSEIENS
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKP-SSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of Spg009653.1 vs. NCBI nr
Match: XP_038893327.1 (probable membrane-associated kinase regulator 4 [Benincasa hispida])

HSP 1 Score: 580.5 bits (1495), Expect = 9.8e-162
Identity = 307/350 (87.71%), Postives = 321/350 (91.71%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPS DP+DDDYIDME+GSYTNF C SKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDMEIGSYTNFHCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQM+EKLLQKSSSDYDYRKD FEEFY+TPL+TSVNTP  TSTPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLSTSVNTP--TSTPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEYLLEY+ DVSYLN ES KKSWTKK KHS+L LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGESLKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTKSGCSDESCAAA KIADEG ASKANE SKK+AKVEKRKPFG   SSIAN NK+K
Sbjct: 181 IKSLFTKSGCSDESCAAAAKIADEGSASKANEYSKKSAKVEKRKPFGPISSSIANSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT+NSSRNRRSFSM IKRQPNIK  SSA  +PSS+SCPN SS  +LKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSMVIKRQPNIKSPSSAPQYPSSSSCPNISSRNYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQEIPELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEIPELCRG 348

BLAST of Spg009653.1 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.7e-66
Identity = 174/362 (48.07%), Postives = 224/362 (61.88%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMS-LNSRGREPTTSPAD 60
           MA  +   D  ++DYIDMEV S+TN L     S    REFEFQMS L     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTN-LVRKTLSNNYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTT-SVNTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+K+L+      DY  D  +EFYSTPL T +V TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILE------DYTFD--DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FESCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKK---FKHSSLGLKLK 180
           FESC +SP+ESC++S+ELNP++Y LEY++ +     +  KKSWT K    K SSLG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKSGCSDE-SCA-AATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSI 240
           ASRAY++S F K+ CSDE SCA +A ++ADE              +  + KPFGQ  +  
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADE----------DSVLRYSRVKPFGQIKT-- 240

Query: 241 ANLNKEKANTDNSSRNRRSFSMAIKRQ----PNIKPSSSASLHPSSASCPNNSSGYFLKR 300
               K+++N   S  +RRSFS++++RQ     N K S+S    P            FLKR
Sbjct: 241 -ERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNNKSSNSLGFRPLQ----------FLKR 300

Query: 301 CGSVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELC 351
             S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS++ +A  DDQE  ++ 
Sbjct: 301 STSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMF 328

BLAST of Spg009653.1 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.9e-32
Identity = 133/352 (37.78%), Postives = 170/352 (48.30%), Query Frame = 0

Query: 12  DDDYIDMEV----------GSYTNFLCHSKSSPRQSREFEFQMSLNS-RGREPTTSPADE 71
           DD YIDMEV           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 131
           LFY G+LLPLH PPRL+MV+KLL  SSS              TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSS--------TAATETPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSESC--RISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASR 191
           SC I   E+C   IS EL        + E     N      SW+KK KHSS+  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTELK------RFIES----NENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNK 251
           AYIK+LF+K  CSD S                          E    F   PS ++    
Sbjct: 198 AYIKALFSKQACSDSS--------------------------EINPRFKIEPSKVSRKKN 257

Query: 252 EKANTDNSSR-NRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEI 311
              N++N    +RRSFS  I+R    K S+S+S   SS+S  + SS +     GS++ + 
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSSS---SSSSASSLSSSFSFGSNGSLDLQT 317

Query: 312 --------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDD 342
                   +NSI+GAI HCKQS + +  +S  T SE+     S TS++   D
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGD 317

BLAST of Spg009653.1 vs. ExPASy Swiss-Prot
Match: Q3E936 (Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 GN=MAKR1 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.9e-07
Identity = 91/332 (27.41%), Postives = 136/332 (40.96%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVEKL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL +V  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFESCNISPSESCRISRELNPDEYLLEYAE 149
           SDY     +     +   +T  N+ T ++  F   +  P + C  SR  +         +
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSRPSS-------VTD 134

Query: 150 DVSYLNAESSKKSWTKKFKHSSLGLKLKASRAYIKSLFTKSGCSDESCAAATKIADEGLA 209
           D  +       KS +  F  S      K       +L   S  S  +  AA   + + ++
Sbjct: 135 DEDFFFKPPKNKSSSGGFSLSRFSSVFKKDPK--TNLHHHSSSSSTATTAAAPSSVKRMS 194

Query: 210 SKANECSKKAAKVEKRKPFGQTPSSIANLNKEKANT--DNSSRNRRSFSMAIKRQPNIKP 269
           S A E  +K  K  K      +P   +N+  E +++  D+ +  R + ++          
Sbjct: 195 STAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIRGTTTVTTVTAAPTVV 254

Query: 270 SSSASLHPS--------------SASCPN------NSSGYFLK--------RCGSVNS-- 320
           SS   L  S              +ASCP+      N SG   +         C S +S  
Sbjct: 255 SSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGGFPVHQGSCSSSSSNN 314

BLAST of Spg009653.1 vs. ExPASy TrEMBL
Match: A0A6J1CCE6 (probable membrane-associated kinase regulator 4 OS=Momordica charantia OX=3673 GN=LOC111010211 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.0e-169
Identity = 313/350 (89.43%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYDPADDDYIDMEV SYTNFLCHSKSSP + REFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSYDPADDDYIDMEVSSYTNFLCHSKSSPPRPREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKS+SDYD RKD FEEFYSTPLTT+VNTPTATSTPFE
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSNSDYDCRKDNFEEFYSTPLTTAVNTPTATSTPFE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSESCRISRELNPDEYLLEY+ D +YLN ESSKKSWT+K KHSSLGLKLKASRAY
Sbjct: 121 SCNISPSESCRISRELNPDEYLLEYSADATYLNGESSKKSWTRKLKHSSLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTKSGCSDESCAAATKIADEG ASKA+ECSKK AKVEKRKP GQ PSSIAN NKEK
Sbjct: 181 IKSLFTKSGCSDESCAAATKIADEGSASKAHECSKKCAKVEKRKPLGQIPSSIANFNKEK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT+N  RNRRSFSM IKRQP+IKPSSSASLH SS+S  +NSS YFLKRCGSVNSEIENS
Sbjct: 241 GNTENRDRNRRSFSMTIKRQPDIKPSSSASLHTSSSSYSHNSSAYFLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQS SQQP RSRKTVSEVGIYLLSS+SLAVS DQEIPELCRG
Sbjct: 301 IQGAIAHCKQSHSQQPFRSRKTVSEVGIYLLSSSSLAVSGDQEIPELCRG 350

BLAST of Spg009653.1 vs. ExPASy TrEMBL
Match: A0A6J1GXX8 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111458502 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.9e-165
Identity = 303/350 (86.57%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPSYDP+DDDYIDME+GSYTNFLC SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVE+LLQKSSSDYD+RKD FEEF+STPLTTS+NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEY LEY+ D SYLN ESSKKSWTKK K S+LGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTK+GCSDESCA A KIADEG ASKANECSKK AKVEKRKPFG   SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT++S  NRRSFS+ IKRQPNIKP SSASL+P+ +SCPNNSSGY+LKRCGSVNSEIENS
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKP-SSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of Spg009653.1 vs. ExPASy TrEMBL
Match: A0A1W6RZ58 (Membrane-associated kinase regulator 4 OS=Cucurbita pepo OX=3663 GN=MAKR4 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.1e-161
Identity = 299/350 (85.43%), Postives = 320/350 (91.43%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSYD ADDDYIDME+G Y+NFLCHSKSSPRQSREFEFQMS N+R REPTTSPADE
Sbjct: 1   MAVDIPSYDSADDDYIDMEIGPYSNFLCHSKSSPRQSREFEFQMSSNTREREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYR D FEEFYSTP +T+VN+PTATSTP E
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRIDIFEEFYSTPFSTAVNSPTATSTPLE 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSESC+ISRELNPDEYLLEY+ D SYLN ESSKKSWTKK KHSSL LKLKASRAY
Sbjct: 121 SCNISPSESCQISRELNPDEYLLEYSSDTSYLNGESSKKSWTKKLKHSSLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTKSGCS+ES AAATKIADEGLASKANECSKK AK+EKRKP GQ P SIAN NKEK
Sbjct: 181 IKSLFTKSGCSEESTAAATKIADEGLASKANECSKKGAKLEKRKPLGQIPISIANSNKEK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            N D+   NRRSFS+AIKRQPNIKPSSSA+LHP+S+SCPN+S GYF K CG +NSE ENS
Sbjct: 241 GNMDH---NRRSFSLAIKRQPNIKPSSSATLHPTSSSCPNDSKGYFQKVCGGMNSEFENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           +QGAIAHCKQSQSQQPL S+KTVSEVGIYLLSS++LAVSDDQEIPELCRG
Sbjct: 301 VQGAIAHCKQSQSQQPLHSKKTVSEVGIYLLSSSTLAVSDDQEIPELCRG 347

BLAST of Spg009653.1 vs. ExPASy TrEMBL
Match: A0A6J1JSI7 (probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=LOC111489379 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.8e-161
Identity = 302/350 (86.29%), Postives = 321/350 (91.71%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DP+DDDYIDME+GSYTNFLC SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSSDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQMVEKL QKSSSDYD+RKD FEEF STPLTTS+NTPTATSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLWQKSSSDYDFRKDTFEEF-STPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELN DEY LEY+ D SYLN ESSKKSWTKK K S+LGLKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNSDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRTY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKSLFTK+GCSDESCA A KIADEG ASKANECSKK AKVEKRKPFG   SSI+N NK+K
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT++SS NRRSFS+ IKRQPNIKP SSASL+P+S+SCPNNSSGY+LKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKP-SSASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS+SL VSDDQE PELCRG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 348

BLAST of Spg009653.1 vs. ExPASy TrEMBL
Match: A0A5A7V1W4 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00070 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.5e-160
Identity = 301/350 (86.00%), Postives = 319/350 (91.14%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPS DP+DDDYID+E+GSYTNF C SKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 120
           LFYNGKLLPLHHPPRLQM+EKLLQKSSSDYDYRKD FEEFY+TPLTTS+NTP  TSTPF+
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120

Query: 121 SCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASRAY 180
           SCNISPSES RISRELNPDEYLLEY+ DVSYLN E SKKSWTKK KHS+L LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNKEK 240
           IKS FTKSGCSDESCAAA KIADEG ASK NECSKK A+VEKRKPFG   SSIAN NK+K
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240

Query: 241 ANTDNSSRNRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEIENS 300
            NT+NSSRNRRSFS+ IKRQP IK SS A LHP+S+SCPN SSG +LKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELCRG 351
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSS++L VSDDQEIPEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of Spg009653.1 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 253.1 bits (645), Expect = 3.4e-67
Identity = 174/362 (48.07%), Postives = 224/362 (61.88%), Query Frame = 0

Query: 1   MAVDIPSYDPADDDYIDMEVGSYTNFLCHSKSSPRQSREFEFQMS-LNSRGREPTTSPAD 60
           MA  +   D  ++DYIDMEV S+TN L     S    REFEFQMS L     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTN-LVRKTLSNNYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTT-SVNTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+K+L+      DY  D  +EFYSTPL T +V TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILE------DYTFD--DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FESCNISPSESCRISRELNPDEYLLEYAEDVSYLNAESSKKSWTKK---FKHSSLGLKLK 180
           FESC +SP+ESC++S+ELNP++Y LEY++ +     +  KKSWT K    K SSLG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKSGCSDE-SCA-AATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSI 240
           ASRAY++S F K+ CSDE SCA +A ++ADE              +  + KPFGQ  +  
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADE----------DSVLRYSRVKPFGQIKT-- 240

Query: 241 ANLNKEKANTDNSSRNRRSFSMAIKRQ----PNIKPSSSASLHPSSASCPNNSSGYFLKR 300
               K+++N   S  +RRSFS++++RQ     N K S+S    P            FLKR
Sbjct: 241 -ERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNNKSSNSLGFRPLQ----------FLKR 300

Query: 301 CGSVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSTSLAVSDDQEIPELC 351
             S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS++ +A  DDQE  ++ 
Sbjct: 301 STSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMF 328

BLAST of Spg009653.1 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 139.0 bits (349), Expect = 7.1e-33
Identity = 133/352 (37.78%), Postives = 170/352 (48.30%), Query Frame = 0

Query: 12  DDDYIDMEV----------GSYTNFLCHSKSSPRQSREFEFQMSLNS-RGREPTTSPADE 71
           DD YIDMEV           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVEKLLQKSSSDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFE 131
           LFY G+LLPLH PPRL+MV+KLL  SSS              TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSS--------TAATETPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSESC--RISRELNPDEYLLEYAEDVSYLNAESSKKSWTKKFKHSSLGLKLKASR 191
           SC I   E+C   IS EL        + E     N      SW+KK KHSS+  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTELK------RFIES----NENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKSGCSDESCAAATKIADEGLASKANECSKKAAKVEKRKPFGQTPSSIANLNK 251
           AYIK+LF+K  CSD S                          E    F   PS ++    
Sbjct: 198 AYIKALFSKQACSDSS--------------------------EINPRFKIEPSKVSRKKN 257

Query: 252 EKANTDNSSR-NRRSFSMAIKRQPNIKPSSSASLHPSSASCPNNSSGYFLKRCGSVNSEI 311
              N++N    +RRSFS  I+R    K S+S+S   SS+S  + SS +     GS++ + 
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSSS---SSSSASSLSSSFSFGSNGSLDLQT 317

Query: 312 --------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSTSLAVSDD 342
                   +NSI+GAI HCKQS + +  +S  T SE+     S TS++   D
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGD 317

BLAST of Spg009653.1 vs. TAIR 10
Match: AT5G26230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 57.8 bits (138), Expect = 2.1e-08
Identity = 91/332 (27.41%), Postives = 136/332 (40.96%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVEKL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL +V  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDYDYRKDAFEEFYSTPLTTSVNTPTATSTPFESCNISPSESCRISRELNPDEYLLEYAE 149
           SDY     +     +   +T  N+ T ++  F   +  P + C  SR  +         +
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSRPSS-------VTD 134

Query: 150 DVSYLNAESSKKSWTKKFKHSSLGLKLKASRAYIKSLFTKSGCSDESCAAATKIADEGLA 209
           D  +       KS +  F  S      K       +L   S  S  +  AA   + + ++
Sbjct: 135 DEDFFFKPPKNKSSSGGFSLSRFSSVFKKDPK--TNLHHHSSSSSTATTAAAPSSVKRMS 194

Query: 210 SKANECSKKAAKVEKRKPFGQTPSSIANLNKEKANT--DNSSRNRRSFSMAIKRQPNIKP 269
           S A E  +K  K  K      +P   +N+  E +++  D+ +  R + ++          
Sbjct: 195 STAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIRGTTTVTTVTAAPTVV 254

Query: 270 SSSASLHPS--------------SASCPN------NSSGYFLK--------RCGSVNS-- 320
           SS   L  S              +ASCP+      N SG   +         C S +S  
Sbjct: 255 SSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGGFPVHQGSCSSSSSNN 314