Spg009069.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg009069.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 CYP72A219-like
Location	scaffold2: 28016628 .. 28022945 (-)
Sequence length	1548
RNA-Seq Expression	Spg009069.1
Synteny	Spg009069.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCAAAGATAAGTTCCCCCTCCATCGCAATAATTGAAGATAAGTTCTCTCTGTCTCGATGGATCTTAAGGCTTCAATTTGGATGGTGGTTATGGTGTTGTTATCGAGATGGGGATGGAAATTTTTGAATTGGATTTGGTTGAAGCCGAAGAAGCTTGAAAAGTGCCTGAGAGAGCAAGGATTCGCCGGAAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACAGTGCTGCCATGACGGAGCAGGCCAAATCCAAACCCATGAACTTCTCCCATGATATTGTTCCACGTGTCTTCCCCTCCATACATCAAACCATCCACAAATATGGTAACATCTTCAATTCTTTTTCTAGTTTTATTTTCACTCCTTAACTTTGTCATGGTTCGGTTTTTGCCGTGACCCGACCGTCCAAAACCAACTGAACCGACGTCAATTAGTCAGTTTGGAGGCTAGTTTTGCATTTCGATCGATCGGAGTAAAAAAATTGAAAAATCAACTTACCAATCGAGAGAATTGGAAAAAAAAAAAATTGGGCACCTATCTTAACGAAAAATGGTTTTGAGAAAACCAAGGAAGCCCATGGTTTTCTCAAAACCATTGTTGGTTTTTAAATAAAATACATTTTTTCCCTCCTCTTTCCTCTCCCTTCCCATGTCATTCCATTTTTAATTTCCCTCTTTTTCCTCTCTTCTCTCTCATCTCTTCTCTTTCCACTCTTCTTTTCCTTCTCTCTTCTAAATGAATGCATTTTTAAAGAAAAAATACAAAAAAAAAATTATTATTGAATTCAAGAAATGTGCATATATATATATACTTGATTATCTAACCTTAAATAAGGAAAAAACTTGTCTGCATACCGGTATGTAGGGATCTCCCTGCATACCACCATTAAAACGTTGACAACTAAGAGAGCCAACAAAAAATTCTCATTTTCTCTCTTCTTCTTTCTTACTTGTCAAGATTTTAATGGGGTATATAGGGAGATCTCTACATACGGTATGCAGGCAAGTTTTGTTCCTTAAATAATTGGCACACTATCACAAACACAAAATAGTATGTTATACAATAAATTAATGGTCGATGAAAGTTTACAAAGTTTGTCGAAGAATTAACAAATTAGTCTAATTGAACTAACTTGTGTAAGTCAACATCGTTAAAGGTAAATTGTTTGAAGTTAGAGATGACATTGAGACCAAATTTGAACAACATTTGAAGGATAAAATTTATCATTTTTTGTTTATTTTGCATTGAATAAATACTATAAACTCTTTATAAGAGTTTTATATTTGTTTTAATACATGTTATATATTTTATTATATTATACATACTAAATTTTATTTCAACTAAAAACCAGGAAACAAGAAGCAGTTATATATCAAATACATTTTTGTTTCTCTATCTTTTTTTTATTAAAAAAGAAACATAAATAGTTATCAAACATGTCGTTTCTGTTTTTCGAAAAACAAAAAAATGAGAACGTTATCAAAGAGGTCCTCAAACATCTACCAAATAATCCAAACTAATTTATTGATTGTAAGTGATTTGTTTGTTTTTGTTAAGAATATTCAACATAGAAAAGCTTGGTAGAAGACAAAACTTGCAATAGAATAGTTGTTGTAAGTCAAAATTCTTATCTAATATAGGGAAGAAGTCGTTCATGTGGTTTGGAGCGTATCCAAGAGTGCACGTGGTAGACCCTGAACAATTGAAAGCTGCTGTTTGTCTGGTCTATGATATTCAAAAGCCAGGTATCAACCCCTTTCTTAGGTTTCTTCTCAGTGGACTTGTTAGTCTTGAAGGACAAAAATGGGCAAAACACAGAAAGATAATCAACCCTGCCTTTCATTTCCAAAAGTTGAAGGTATGTTTCATTTCCCTTTATCTCTTCACAAATTTCGTGTATGAAAGCAAAAAAAAGGTCAAAATTTCATCATTCTTTTCCAACTCTCTATCTGAAAGAGTAGATAACAAAAACAAAAAAAAAACTTGACTTCGTTTCTAAAATCATGGGAAGAAGGTAAATAACAAAATAAAGAAATTTAGACATGGAAGTAATGTTTATAAACTTAATTTTCAAAAGCAAAAATCGAAAAATCAAATAGACATCAAACGGGGCATAAATGATTATCGAACGGGGACCTTTGTTTTTAGTTTTATGTTTTTGAAATTTATGTTTGTTTTCTCATAATTTTTCACCTTTATGAAAGAAATATTTGAATTCTTAGTCAAATTCCGAAACCAAAAACAAGTTTTTAAAAGCTACTTGGTTTTAGTTTTCAAAACTTGACTTGGATTTCAAAAATATTTCTAAAAAGTAGATAACAAAACATGAGTGAAATTAGTGTTTATAAACTTAATTTTCAAAAAACCAAAACAAAAAAAACTAAGGCCCCGTTTGATAACCATTTGGTTTTTGAAAATTATGCTTGTTTTCTCCCTATTTTTCCTTACCATGGTTTTCGTATTTGTCAAAGACACGTTTGAATTCCTAGTCAGATTCCAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTAGTTTAAAAAAATTTGCTTGGTTTTTAAAAACATGGAAAGAAGTGTGTTAGAAAAACAAGGAAACTTGTAGGTGGAAGTAGTGTTTATAAGCTTAATTTTCAAAGACAAAAAATCAAGAACCAAATGGTTATCAAACGAGGCCTAAATGATTATCAAACACAATCTACACACTCAAAGAAGTTAGTCATAGACACTATATATTGTTGATCTCTGTACAACATGTCCAACGAACAAAGTTCAATTGGTTCATATAAGTATGTTTACAATATCATATCAAACTAGGATATGTTGTTAAATGACCAATAATCGCAAACCGAAAGCTTAAGCTGATGAGTTATGGTAAATTTAATCTTATATCAAGACTTCAACAATGATATGCACTTATTTCGCCCAAAAAGATGGACAAATTAGAGTAAAGATTTGAATTGCTACAATTTCTAAATCATTATATAGTGTACTCTGAGTAAATTTTGTTGAAAGTCTAAATGTTCATATATACCCTCACATTGACATACAGGGCATGGTGCCAACAATCTGTGAGAGTTGCAATGAGATGATTAACAAATGGGAAAGAATGATCTCAGAAGATGGATTTTGTGAATTGGATGTGATGCCTCATTTGCAAAATTTGGCAGCTGATGTCATTTCTCGAACAGCATTTGGAAGTAGTTATGAAGAAGGAAAAATCATCTTTCAACATCTAAAAGAATTGACAGATTTGGTCCTTAAAGTTACATCTAGAGTTTACATACCGGGATGGAGGTAACATAATATTTTCTAATTCAAAATTACTTGTTTACAAATATGGAAATGTTTAATTGTTTAGGATATAGACATTAGTAAGTATTAAATTCAGCTTTCACTTAGGCTCCGTTTGATAACCATTTCGTTTTCGGTTTTTGGTTTTTGAAAATTAAGCTTATACACACCTACTTTCACTTATTTTAGTATCAACCTTCTTCCTTGTTTTCAAAAATCAAGTCCAATTTTGAAAAAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGGCTAGGAATTCAAATGGATCTTTAATAAGGATGAAAGTCATGGTAGAGAATTTAGGAGAAAACAAGCATAATTTTCAAAAACAAAAAACAAAAAACGAAATGGTTATCAAACGGAGCCTTAATGATTTATTATCCTTATTAAAGACTCGTTTGGAAATACCTTTACGTTTCAATGTTTTTCAAGAATGCATTTTTTAAAAACATTTTGTATAAAAGTTGTTTTGAATTTAAACAATCATATGTTTGGTGACATTTCCTCACAAATGTTTTTATACACAAGTTATTATATACTTAAAAGCTCTTGTTAAAAAATTTGCTTTCAAGTGTTTCTCTCAAAATGGTTTATTTCTCAATCTCATTTTTTAATAAATCATTTTTAGTGGTTGCCAAACACTAGAATTTTTTTCAAAATAGTTTGTTTTCAAAATTAAACACTCGAAAAGTTAAACCAAACATATCCTAATATTATTTCATTGCTTGGATGTTGAGTTCATCAATATATGGATGTGGGAATTTAAACCTTCAACTTTTTAGTCGAGATTATGATACCCTAATCAGTTGTGTTATATTGAAGTTGACATTAATGAGTATCTAATGTTTGCATACTTCATATGTATACCATTCCAATCATGGTTTATTATATATTACTTTTGGTATACCTAATCCATTGAGAACATGAACATGAGTGTATCAAATTTATTATCATTTGAGAAGTATTATTTCATCTCATTATTAGCTTTGTGCAGGTTTCTACCAACCAAGTCTAACAATAGAATGAAAAAGATAAGTAAAGAAATAAAAACTGTGCTGTTTAGCCTTGTCAATAAAAGGCAAATGAGTATGAAGGCAGGTGACACAGTACATAATAATGATCTATTAAGCATCCTCCTAGAATCAAATTTCAAAGAAATTGAAGAACATGGAAGTAACGATATTGGAATGAGCATAGAAGACATGATCGAAGAATGTAAGGTTTTCTATTTTGCTGGCCAAGAAACCACAGCATTGTTACTAGTTTGGACGATGGTTTTGTTGAGCTCAAATCCAAAATGGCAAGAGCGAGCAAGAGCTGAGGTCCTAGAAGTTTTTGGTAACAACAAACCAGATTTTGATGGTTTAAATCGCCTAAAAATTGTGAGTGAGATTTTTTTTTTCTTGTATTTGTTCTCTCCTTTTTTTTTGTTTCATTCTTTAAATTTGGTCTCATATATACCCAAATACTTAGGCTGGTTTGATAACTATTTCGTTTTAGGTTTTTTTGGTTTTTGAAAATTATGCTTGCTAATTTCTTCATAATTTTCATACCTTCTTGGTTTTCATCTTTCTCAAGGGCACATTTAAATTCTTAGCCAAATTTCGAAAACAAAAACAAGTTTTTGAAAACTACGACTCTGTTTGATAATCATTTGGTTTTTGGTTTTTTGTTTTTGAAAATTATGTTTATAAACACTACCTCCACTTCTAAGTTTATTTATTATTATTATTATTATTATTATTATTATTATTTGTTATCTACCTTCTTCCCATGTTTTCAAAAACCAAGTCAATTTCTAAATACTAAGGTTCCGTTTGATAATCATTTGGTTTTTGAAAATTATAGTTGTTTTCTCCTAAATTTCCTACCATGGCTTTCACCCTAGTTAAGGTTGCATATTTGGTTTTTGAAAATTATAGTTGTTTTCTCCTAAATTTCCTACCATGGCTTTCACCCTAGTTAAGGTTGCATTTGAACTCCTAACTAAATTTTAAAAACAAAAGCAAGTTTTTGAAAACTACTTTTTTTTTTGTTTCCAAATTTGGACTTGGTTTTTGGAAACAAGGTAGAAGATTGAGACTAAAACCAAGAAACTTAAGCTTAATTTTCAAAAACTAAAAACCAAATGGTTATCAAACGGGGCCTAAAAAAGTAGTTTTCAATTTTTATTATTTTTTTTTTTTGGAATTTGGCGCTAAGAATTCAAGTGTGTCCTTAATAAAGATGAAAACCATGGTTAGAAAATTGGAAGAAAACAAACATAATTTTAAAAAACTAAAAACAAAAAACCAAACAATTATCAAACAAGACCCTATTTTTTAGTTTTCAAAACTTGAGTTGGTTTTTTAAAACATAAGAAGTAGATAACAAAACAAAGAAACTTATAGGTGGGTAGAAATAGTATTTATAGGCTTAATTAATTTTCAAAAACTAAAATTCAAAAACAAAATAGTTATCAAACGACGTCTTAAATACTCAGTTACTTGTTCTAAACTCTTGCATTAGTTATTTTGGCAATGAACAAGCATAACACAACATGTTATGTTTGCAATGTGGTAGGTTAGTATGATTTTGAACGAGGTTCTTAGATTATACCCACCGGGAGGAATGTCTAGTCGTCTTATTAAAAAGGAAACAAGACTTGGAAAAGTGAGGCTGCCGGCTGGAGTGATGTTGTACTTTCCAATTGCTCTTATCCATCGCGATCAAGAGCTATGGGGTGAGGATGCAAATGAATTCAATCCACAAAGATTCGCTGAAGGAGTTTCGAAAGCAACACAGAATCAAGCTGGGTTCTTCCCATTTGGATGGGGTCCTCGAATATGCATCGGACAAAATTTTGCCATGATTGAGGCCAAAATTGCACTGTCCATGATCCTACAACACTTCAAATTTGAACTTTCTTCTTCCTATACTCATGCTCCTACATTTGTTTTTACTATTCAACCTCAATATGGAGCTCATATCATCCTACGTAAACTTTAG

mRNA sequence

ATGGATCTTAAGGCTTCAATTTGGATGGTGGTTATGGTGTTGTTATCGAGATGGGGATGGAAATTTTTGAATTGGATTTGGTTGAAGCCGAAGAAGCTTGAAAAGTGCCTGAGAGAGCAAGGATTCGCCGGAAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACAGTGCTGCCATGACGGAGCAGGCCAAATCCAAACCCATGAACTTCTCCCATGATATTGTTCCACGTGTCTTCCCCTCCATACATCAAACCATCCACAAATATGGGAAGAAGTCGTTCATGTGGTTTGGAGCGTATCCAAGAGTGCACGTGGTAGACCCTGAACAATTGAAAGCTGCTGTTTGTCTGGTCTATGATATTCAAAAGCCAGGTATCAACCCCTTTCTTAGGTTTCTTCTCAGTGGACTTGTTAGTCTTGAAGGACAAAAATGGGCAAAACACAGAAAGATAATCAACCCTGCCTTTCATTTCCAAAAGTTGAAGGGCATGGTGCCAACAATCTGTGAGAGTTGCAATGAGATGATTAACAAATGGGAAAGAATGATCTCAGAAGATGGATTTTGTGAATTGGATGTGATGCCTCATTTGCAAAATTTGGCAGCTGATGTCATTTCTCGAACAGCATTTGGAAGTAGTTATGAAGAAGGAAAAATCATCTTTCAACATCTAAAAGAATTGACAGATTTGGTCCTTAAAGTTACATCTAGAGTTTACATACCGGGATGGAGGTTTCTACCAACCAAGTCTAACAATAGAATGAAAAAGATAAGTAAAGAAATAAAAACTGTGCTGTTTAGCCTTGTCAATAAAAGGCAAATGAGTATGAAGGCAGGTGACACAGTACATAATAATGATCTATTAAGCATCCTCCTAGAATCAAATTTCAAAGAAATTGAAGAACATGGAAGTAACGATATTGGAATGAGCATAGAAGACATGATCGAAGAATGTAAGGTTTTCTATTTTGCTGGCCAAGAAACCACAGCATTGTTACTAGTTTGGACGATGGTTTTGTTGAGCTCAAATCCAAAATGGCAAGAGCGAGCAAGAGCTGAGGTCCTAGAAGTTTTTGGTAACAACAAACCAGATTTTGATGGTTTAAATCGCCTAAAAATTGTTAGTATGATTTTGAACGAGGTTCTTAGATTATACCCACCGGGAGGAATGTCTAGTCGTCTTATTAAAAAGGAAACAAGACTTGGAAAAGTGAGGCTGCCGGCTGGAGTGATGTTGTACTTTCCAATTGCTCTTATCCATCGCGATCAAGAGCTATGGGGTGAGGATGCAAATGAATTCAATCCACAAAGATTCGCTGAAGGAGTTTCGAAAGCAACACAGAATCAAGCTGGGTTCTTCCCATTTGGATGGGGTCCTCGAATATGCATCGGACAAAATTTTGCCATGATTGAGGCCAAAATTGCACTGTCCATGATCCTACAACACTTCAAATTTGAACTTTCTTCTTCCTATACTCATGCTCCTACATTTGTTTTTACTATTCAACCTCAATATGGAGCTCATATCATCCTACGTAAACTTTAG

Coding sequence (CDS)

ATGGATCTTAAGGCTTCAATTTGGATGGTGGTTATGGTGTTGTTATCGAGATGGGGATGGAAATTTTTGAATTGGATTTGGTTGAAGCCGAAGAAGCTTGAAAAGTGCCTGAGAGAGCAAGGATTCGCCGGAAATCCTTACCGGCTTTTCTCCGGCGACATGAAGGACAGTGCTGCCATGACGGAGCAGGCCAAATCCAAACCCATGAACTTCTCCCATGATATTGTTCCACGTGTCTTCCCCTCCATACATCAAACCATCCACAAATATGGGAAGAAGTCGTTCATGTGGTTTGGAGCGTATCCAAGAGTGCACGTGGTAGACCCTGAACAATTGAAAGCTGCTGTTTGTCTGGTCTATGATATTCAAAAGCCAGGTATCAACCCCTTTCTTAGGTTTCTTCTCAGTGGACTTGTTAGTCTTGAAGGACAAAAATGGGCAAAACACAGAAAGATAATCAACCCTGCCTTTCATTTCCAAAAGTTGAAGGGCATGGTGCCAACAATCTGTGAGAGTTGCAATGAGATGATTAACAAATGGGAAAGAATGATCTCAGAAGATGGATTTTGTGAATTGGATGTGATGCCTCATTTGCAAAATTTGGCAGCTGATGTCATTTCTCGAACAGCATTTGGAAGTAGTTATGAAGAAGGAAAAATCATCTTTCAACATCTAAAAGAATTGACAGATTTGGTCCTTAAAGTTACATCTAGAGTTTACATACCGGGATGGAGGTTTCTACCAACCAAGTCTAACAATAGAATGAAAAAGATAAGTAAAGAAATAAAAACTGTGCTGTTTAGCCTTGTCAATAAAAGGCAAATGAGTATGAAGGCAGGTGACACAGTACATAATAATGATCTATTAAGCATCCTCCTAGAATCAAATTTCAAAGAAATTGAAGAACATGGAAGTAACGATATTGGAATGAGCATAGAAGACATGATCGAAGAATGTAAGGTTTTCTATTTTGCTGGCCAAGAAACCACAGCATTGTTACTAGTTTGGACGATGGTTTTGTTGAGCTCAAATCCAAAATGGCAAGAGCGAGCAAGAGCTGAGGTCCTAGAAGTTTTTGGTAACAACAAACCAGATTTTGATGGTTTAAATCGCCTAAAAATTGTTAGTATGATTTTGAACGAGGTTCTTAGATTATACCCACCGGGAGGAATGTCTAGTCGTCTTATTAAAAAGGAAACAAGACTTGGAAAAGTGAGGCTGCCGGCTGGAGTGATGTTGTACTTTCCAATTGCTCTTATCCATCGCGATCAAGAGCTATGGGGTGAGGATGCAAATGAATTCAATCCACAAAGATTCGCTGAAGGAGTTTCGAAAGCAACACAGAATCAAGCTGGGTTCTTCCCATTTGGATGGGGTCCTCGAATATGCATCGGACAAAATTTTGCCATGATTGAGGCCAAAATTGCACTGTCCATGATCCTACAACACTTCAAATTTGAACTTTCTTCTTCCTATACTCATGCTCCTACATTTGTTTTTACTATTCAACCTCAATATGGAGCTCATATCATCCTACGTAAACTTTAG

Protein sequence

MDLKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL

Homology

[Close]

BLAST of Spg009069.1 vs. NCBI nr
Match: XP_038878667.1 (cytochrome P450 CYP72A219-like isoform X1 [Benincasa hispida])

HSP 1 Score: 835.1 bits (2156), Expect = 3.3e-238
Identity = 396/516 (76.74%), Postives = 451/516 (87.40%), Query Frame = 0


Query: 1   MDLKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAM 60
           MD   SIWMV+MV L  WGW+FLNWIWL+PKKLEK LR+QGFAGN YRL+SGD+ D AA+
Sbjct: 1   MDFSDSIWMVIMVCLVGWGWRFLNWIWLRPKKLEKWLRQQGFAGNSYRLYSGDVNDMAAV 60

Query: 61  TEQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVY 120
            EQAKSKPM+FSHDI PRV PSIH TIHKYGK SF W G YPRV+++DPEQLKAA  L  
Sbjct: 61  MEQAKSKPMSFSHDIAPRVIPSIHHTIHKYGKNSFTWLGPYPRVYIMDPEQLKAAFSLYT 120

Query: 121 DIQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKW 180
           D QKP INP  R L  GL++ EG KWAKHRKIINPAFHF+KLK MVPT+ ESC+EMI+KW
Sbjct: 121 DFQKPTINPLGRLLFDGLINHEGAKWAKHRKIINPAFHFEKLKDMVPTMFESCSEMISKW 180

Query: 181 ERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVY 240
           ERMIS+DGFCELDVMPHLQNLAADVISRTAFGS+YEEGKIIF  LKELT+LV+K ++ VY
Sbjct: 181 ERMISKDGFCELDVMPHLQNLAADVISRTAFGSTYEEGKIIFNLLKELTNLVIKTSNTVY 240

Query: 241 IPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEI 300
           IPGWRFLPTK NNRMKKISKE+KT+L +L+NKRQ +MK G+ +H +DLL ILLESNF EI
Sbjct: 241 IPGWRFLPTKLNNRMKKISKEMKTLLLNLINKRQRAMKEGEAIH-SDLLGILLESNFNEI 300

Query: 301 EEHGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFG 360
           +EHG+ +IGMSI+D++EECKVFY AGQETTA+LLVWTMVLLSS  KWQ+RAR+EVL+VFG
Sbjct: 301 QEHGNVNIGMSIDDLVEECKVFYLAGQETTAILLVWTMVLLSSYTKWQDRARSEVLQVFG 360

Query: 361 NNKPDFDGLNRLKIVSMILNEVLRLYPPGGM-SSRLIKKETRLGKVRLPAGVMLYFPIAL 420
            NKPDFDGLNRLK+V+MILNEVLRLYPP  + S RL+KKETRLG + LP+GV+L FPI L
Sbjct: 361 YNKPDFDGLNRLKVVTMILNEVLRLYPPASVFSRRLVKKETRLGNLTLPSGVLLSFPILL 420

Query: 421 IHRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSM 480
           IH+D ELWGEDANEFNP+RFAEG+SKAT+NQ GFFPFGWGPRIC+GQNFAMIEAKIALSM
Sbjct: 421 IHQDPELWGEDANEFNPERFAEGISKATKNQTGFFPFGWGPRICVGQNFAMIEAKIALSM 480

Query: 481 ILQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           ILQHF FELSSSYTHAP  + TIQPQ+GAHIIL KL
Sbjct: 481 ILQHFSFELSSSYTHAPISILTIQPQHGAHIILHKL 515

[Close]

BLAST of Spg009069.1 vs. NCBI nr
Match: XP_022933047.1 (cytochrome P450 CYP72A219-like [Cucurbita moschata])

HSP 1 Score: 816.2 bits (2107), Expect = 1.6e-232
Identity = 381/513 (74.27%), Postives = 440/513 (85.77%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SIW V +V L+ WGW+FLNWIWL+PKKLE+ LR QGFAGNPYRL SGDMKD AAM E
Sbjct: 7   IRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERSLRNQGFAGNPYRLLSGDMKDIAAMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PSI+ TIHKYGK SFMW G YPRVH++DP+QLKAA+ L++D+
Sbjct: 67  QAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAALSLMHDV 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           QKP INP  R L  GL   EG KW KHRKIINPAFHF+ LKGMVP +CESC EMI KWE 
Sbjct: 127 QKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
           ++S+DG+ ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +LV+K TS VYIP
Sbjct: 187 VVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNNRMKKISKE++ +L  ++NKRQ +M AG  +H NDLLSILL+SN KEI E
Sbjct: 247 GWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMH-NDLLSILLQSNLKEIRE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           HG+ND+GMSIED+++EC+VFY AGQETTA+LL+WTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HGNNDVGMSIEDIVDECRVFYLAGQETTAMLLIWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLN LKIV+MILNEVLRLYPP  + SRL++ ET+LGK+  PAGVML FPI LIHR
Sbjct: 367 KPDFDGLNGLKIVTMILNEVLRLYPPAALVSRLVQNETKLGKLSFPAGVMLSFPIILIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D++LWG+DANEFNP RF EGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKKLWGDDANEFNPDRFTEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSYTHAPT +   +PQ+GAHI+LRKL
Sbjct: 487 HFSFELSSSYTHAPTSILITRPQHGAHIMLRKL 518

[Close]

BLAST of Spg009069.1 vs. NCBI nr
Match: XP_022974803.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 808.1 bits (2086), Expect = 4.3e-230
Identity = 380/513 (74.07%), Postives = 438/513 (85.38%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SIW V +V L+ WGW+FLNWIWL+PKKLE+CLR+QGFAGNPYRL +GDMKD AAM E
Sbjct: 7   IRDSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKDIAAMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PSI+ T HKYGK SFMW G YPRVH++DP+QLKAA  L++D+
Sbjct: 67  QAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAFSLMHDV 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           QKP INP  R L  GL   EG KW KHRKIINPAFHF++LK MVP +CESC EMI KWE 
Sbjct: 127 QKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
           ++S+D + ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +LV+K TS VYIP
Sbjct: 187 VVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNN MKKISKE+KT+L  ++NKRQ +M AG+ +H NDLLSILL+SN KEI E
Sbjct: 247 GWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAMH-NDLLSILLQSNLKEILE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           HG+ND+GMSIED+++EC+VFY AGQETTA+LLVWTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HGNNDVGMSIEDIVDECRVFYLAGQETTAMLLVWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLNRLKIV+MILNEVLRLYPP  + SRL++ E +LGK+  PAGVML FPI LIHR
Sbjct: 367 KPDFDGLNRLKIVTMILNEVLRLYPPPALISRLVQNEIKLGKLSFPAGVMLSFPIILIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D+ LWG+DANEFNP RFAEGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKNLWGDDANEFNPDRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSY HAPT   T +PQ+GAHI+L KL
Sbjct: 487 HFSFELSSSYKHAPTSNLTTRPQHGAHIMLLKL 518

[Close]

BLAST of Spg009069.1 vs. NCBI nr
Match: XP_023516285.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516286.1 cytochrome P450 CYP72A219-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023516287.1 cytochrome P450 CYP72A219-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.4 bits (2084), Expect = 7.3e-230
Identity = 377/513 (73.49%), Postives = 439/513 (85.58%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SIW V +V L+ WGW+FLNWIWL+PKKLE+CLR+QGFAGNPYRL +GDMKD A M E
Sbjct: 7   IRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKDIATMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PSI+ TIHKYGK SFMW G YP VH++DP+QLKAA+ L++D+
Sbjct: 67  QAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPSVHIMDPDQLKAALSLMHDV 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           +KP INP  R L  GL   EG KW KHRKIINPAFHF++LK MVP +CESC EMI KWE 
Sbjct: 127 RKPSINPLQRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
            +S+DG+ ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +L +K TS VYIP
Sbjct: 187 GVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLFVKATSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNNRMKKISKE+K +L  ++NKRQ +M AG+ +H NDLLSILL+SN KEI+E
Sbjct: 247 GWRFVPTKSNNRMKKISKELKALLLDVINKRQRAMMAGEAMH-NDLLSILLQSNLKEIQE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           HG+ND+GMSI+D+++EC+VFY AGQETTA+LLVWTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HGNNDVGMSIKDIVDECRVFYLAGQETTAMLLVWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLNRLKIV+MILNEVLRLYPP  + SRL++ ET+L K+  PAGVML FPI  IHR
Sbjct: 367 KPDFDGLNRLKIVTMILNEVLRLYPPAAVVSRLVQNETKLEKLSFPAGVMLSFPIIFIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D++LWG+DANEFNP RFAEGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKKLWGDDANEFNPDRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSYTHAPT   T +PQ+GAHI+L KL
Sbjct: 487 HFSFELSSSYTHAPTSNLTTRPQHGAHIMLCKL 518

[Close]

BLAST of Spg009069.1 vs. NCBI nr
Match: XP_023520477.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.0 bits (2083), Expect = 9.5e-230
Identity = 379/513 (73.88%), Postives = 440/513 (85.77%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SI  V++V L+ WGW++LNWIWL+PKKLE+CLR+QGFAGNPYRL +GD+KD AAM E
Sbjct: 7   IRVSILAVIVVWLAVWGWRYLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDIKDIAAMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PS++ TIH YGK SFMW G YPRVH++DP+QLKAA  L++DI
Sbjct: 67  QAISSPMNFSHDIAPRVIPSMNHTIHTYGKNSFMWIGPYPRVHIMDPDQLKAAFSLMHDI 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           QKP INP  R L  GL   EG KW KHRKIINPAFHF+ LK MVP +CESC EMI KWE 
Sbjct: 127 QKPSINPLSRLLFDGLADHEGPKWVKHRKIINPAFHFKHLKDMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
           ++S+DG+ ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +L++KV S VYIP
Sbjct: 187 LVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLIVKVVSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNNRMKKISKE+KT+L +++NKRQ +M AG+ +H NDLLSILL+SN KEI E
Sbjct: 247 GWRFVPTKSNNRMKKISKELKTLLLNVINKRQRAMMAGEAMH-NDLLSILLQSNLKEIRE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           H +ND+GMSI+D++ EC+VFY AGQETTA+LLVWTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HENNDVGMSIKDIVNECRVFYLAGQETTAMLLVWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLNRLKIV+MILNEVLRLYPP    SRL++ ET+L K+  PAGVML FPI LIHR
Sbjct: 367 KPDFDGLNRLKIVTMILNEVLRLYPPVAFFSRLVQNETKLEKLSFPAGVMLSFPIILIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D++LWG+DANEFNP RFAEGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKKLWGDDANEFNPDRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSYTHAPT   T +PQYGAHI+LRKL
Sbjct: 487 HFSFELSSSYTHAPTSNLTTRPQYGAHIMLRKL 518

[Close]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.2e-180
Identity = 291/515 (56.50%), Postives = 388/515 (75.34%), Query Frame = 0


Query: 1   MDLKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAM 60
           + L +S   +V+V+L   GW+  NW+WL+P+KLEK LR QGF GN YRLF GD+K+   M
Sbjct: 5   LKLISSFCAIVVVIL--LGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVM 64

Query: 61  TEQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVY 120
            ++AKSKP+N   DI+PR+ P   + I  YGK SF+W G  P VH+++P+ +K  +   Y
Sbjct: 65  LKEAKSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFY 124

Query: 121 DIQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKW 180
             QKP  NP  + L +G+   EG +WAKHRK+INPAFH +KLK M+P I  S +E++ KW
Sbjct: 125 QFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKW 184

Query: 181 ERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVY 240
           E M+S  G  ELDV+P+L+ L +DVISRTAFGSSYEEG+ IFQ  +E  +L+++ +  +Y
Sbjct: 185 EEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIY 244

Query: 241 IPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEI 300
           +PG RFLPTK N RMK+I+KE+K  L S++NKR  +M+AG+   ++DLL ILLESN KEI
Sbjct: 245 LPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEI 304

Query: 301 EEHGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFG 360
           ++HG+ + G++++++IEECK+F+FAGQETT+ LLVWTM+LLS +  WQ+RA+ EVL  FG
Sbjct: 305 KQHGNTNFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFG 364

Query: 361 NNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALI 420
           NNKPDFDGLN LK+V+MIL EVLRLYPP     R I +E +LG++ LPAGV+L  PI L+
Sbjct: 365 NNKPDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILL 424

Query: 421 HRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMI 480
           H DQE+WG+DA EFNP+RF+EGV KAT+ +  +FPF WGPRICIGQNFAM+EAK+A++MI
Sbjct: 425 HYDQEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMI 484

Query: 481 LQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           LQ F F LS SY HAP  + T+QPQYGAH+IL  L
Sbjct: 485 LQRFSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

[Close]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 5.5e-180
Identity = 297/512 (58.01%), Postives = 383/512 (74.80%), Query Frame = 0


Query: 4   KASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQ 63
           K +  + V V++  W WK LNW+W+ P+KLE+ LR+QGF GN YRLF GD+K+S+ MT +
Sbjct: 10  KIAAAVAVAVVVVGWAWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMTRK 69

Query: 64  AKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQ 123
           AK KP+N S D V RV P IHQT+ KYGK SF+W G  PRV ++DPE +K  +   Y   
Sbjct: 70  AKLKPINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSYKFN 129

Query: 124 KPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERM 183
           KP  NP ++    GL + EG+ WAKHRK++NPAFH ++LK M+P +  SC EM++KW++M
Sbjct: 130 KPKRNPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKWDKM 189

Query: 184 ISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPG 243
           IS+DG  ELDV P LQ L +DVIS TAFGSSYEEG I+F+   E  +LV+K    VYIPG
Sbjct: 190 ISKDGSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQSVYIPG 249

Query: 244 WRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEH 303
           W +LPTK N +MK+I ++ ++ L +++NK+  +M+AG+    +D+L ILLESN KE    
Sbjct: 250 WSYLPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGEG-STDDILGILLESNLKEQLGQ 309

Query: 304 GSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNK 363
           G  ++GMSI++++ ECK FYFAGQETT+ LLVWTMVLLS +P WQ RAR EVL+ FGN K
Sbjct: 310 GKKNVGMSIQEVMGECKQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQFGNAK 369

Query: 364 PDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRD 423
           PDFD LN LKIV+MIL EVLRLYPP     R + +ET LG + LPAGV +  PI ++H D
Sbjct: 370 PDFDNLNHLKIVTMILYEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMILHHD 429

Query: 424 QELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQH 483
           Q +WG+DA EFNP+RF+EGVSKAT+NQ  FFPFGWGPRICIGQNFA++EAK+AL++ILQ 
Sbjct: 430 QNIWGDDAKEFNPERFSEGVSKATKNQVVFFPFGWGPRICIGQNFALLEAKLALAIILQR 489

Query: 484 FKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           F FELS SYTHAPT V T+QPQ+GA++IL KL
Sbjct: 490 FSFELSPSYTHAPTTVLTVQPQHGANLILHKL 520

[Close]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot
Match: Q2MJ19 (Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 3.7e-168
Identity = 276/515 (53.59%), Postives = 386/515 (74.95%), Query Frame = 0


Query: 2   DLKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMT 61
           + K++I ++ +     + W+ LNW+WLKPKK+EK LREQG  GNPYRL  GD KD   M 
Sbjct: 6   ETKSAIILITVTFGLVYAWRVLNWMWLKPKKIEKLLREQGLQGNPYRLLLGDAKDYFVMQ 65

Query: 62  EQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYD 121
           ++ +SKPMN S DI PRV P IH  +  +GKKSF+WFG  P V + +PEQ++     + +
Sbjct: 66  KKVQSKPMNLSDDIAPRVAPYIHHAVQTHGKKSFIWFGMKPWVILNEPEQIREVFNKMSE 125

Query: 122 IQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWE 181
             K     F++ +  GLV LEG+KW+KHR+IINPAFH +KLK M PT  +SCN++I+ WE
Sbjct: 126 FPKVQYK-FMKLITRGLVKLEGEKWSKHRRIINPAFHMEKLKIMTPTFLKSCNDLISNWE 185

Query: 182 RMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYI 241
           +M+S +G CE+DV P LQ+L +DVI+R++FGSSYEEG+ +FQ   E  +L++K   +  I
Sbjct: 186 KMLSSNGSCEMDVWPSLQSLTSDVIARSSFGSSYEEGRKVFQLQIEQGELIMKNLMKSLI 245

Query: 242 PGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIE 301
           P WRFLPT  + ++ +  K+I+T L +++NKR+ ++KAG+    NDLL +LLESN +EI+
Sbjct: 246 PLWRFLPTADHRKINENEKQIETTLKNIINKREKAIKAGEAT-ENDLLGLLLESNHREIK 305

Query: 302 EHGS-NDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFG 361
           EHG+  ++G+S+E+++ EC++F+ AGQETT+ LLVWTMVLLS  P WQERAR EVLE+FG
Sbjct: 306 EHGNVKNMGLSLEEVVGECRLFHVAGQETTSDLLVWTMVLLSRYPDWQERARKEVLEIFG 365

Query: 362 NNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALI 421
           N KPDFDGLN+LKI++MIL EVLRLYPP    +R ++ + +LG + L AG+ +Y PI LI
Sbjct: 366 NEKPDFDGLNKLKIMAMILYEVLRLYPPVTGVARKVENDIKLGDLTLYAGMEVYMPIVLI 425

Query: 422 HRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMI 481
           H D ELWG+DA  FNP+RF+ G+SKAT  +  +FPFG GPRICIGQNF+++EAK+A+++I
Sbjct: 426 HHDCELWGDDAKIFNPERFSGGISKATNGRFSYFPFGAGPRICIGQNFSLLEAKMAMALI 485

Query: 482 LQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           L++F FELS +Y HAP+ V ++QPQ+GAH+ILRK+
Sbjct: 486 LKNFSFELSQTYAHAPSVVLSVQPQHGAHVILRKI 518

[Close]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 7.7e-166
Identity = 272/501 (54.29%), Postives = 376/501 (75.05%), Query Frame = 0


Query: 20  WKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPMNFSHDIVPRV 79
           WK LNW W+KPKKLEK LR+ G  GN YRL  GD+ +++ M ++AKSKP+NF++ I+PR+
Sbjct: 38  WKMLNWAWIKPKKLEKRLRQLGLKGNSYRLLIGDLGETSQMDKEAKSKPINFTNHIIPRI 97

Query: 80  FPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINPFLRFLLSGLV 139
            P +H+ I  +GK  F+W G  P V + DPE +K  +   Y  QKP  +   + +  G+ 
Sbjct: 98  MPFLHKIIQSHGKNPFIWQGPNPAVIITDPELIKEVLTKNYTYQKPPSDDLGKLISQGVA 157

Query: 140 SLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERM--ISEDGFCELDVMPH 199
           + +  KWAKHR+++NPAFH +KLK M+P    SC+EM+ KWE++   S +G  ELDV P+
Sbjct: 158 AYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCSEMLKKWEKLASASAEGSFELDVWPY 217

Query: 200 LQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLPTKSNNRMKK 259
           LQ++ +DVISRTAFGS+ EEGK IF+  KE+ + +L++    Y PG  +LPTK   RMKK
Sbjct: 218 LQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHILQILQSFYFPGMLYLPTKRIRRMKK 277

Query: 260 ISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIE-EHGSN---DIGMSIE 319
           ISKE+ + + +++ KR  +MKAG+   N+DLL ILL+SNFKEIE ++G+N   + GMS E
Sbjct: 278 ISKEVHSSVLNIIMKRVEAMKAGEP-SNDDLLGILLDSNFKEIENDNGNNSNKNCGMSFE 337

Query: 320 DMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFDGLNRLK 379
           ++IEECK+FYFAGQETTA+LLVWT++LLS +P+WQ RAR EVL++FGN++PDFD LN LK
Sbjct: 338 EVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARDEVLQIFGNSEPDFDALNHLK 397

Query: 380 IVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELWGEDANE 439
           +++M++NEVLRLYPPG M SR+I ++T+LG + LP G+ ++ P  ++H D+ELWG+DA E
Sbjct: 398 VITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGISIFLPTIMLHHDEELWGDDAKE 457

Query: 440 FNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFELSSSYT 499
           F P+RF+EG+SKAT+ Q  +FPF  GPRICIG NFAM+EAK+AL++ILQ F FE+S SYT
Sbjct: 458 FKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFAMLEAKMALALILQRFTFEISPSYT 517

Query: 500 HAPTFVFTIQPQYGAHIILRK 515
           HAP  + T+QPQYGA +IL K
Sbjct: 518 HAPHTLVTLQPQYGAQLILYK 537

[Close]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot
Match: Q05047 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 6.5e-165
Identity = 267/516 (51.74%), Postives = 371/516 (71.90%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ +I   +  L+  W W+ L+W W  PK++EK LR+QGF GNPYR   GD+K+S  M +
Sbjct: 8   IRKAIAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQ 67

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           +A SKPM F++DIVPR+ P I+ TI+ YG+ SF W G  PR+HV++PE +K  +      
Sbjct: 68  EALSKPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKY 127

Query: 123 QK--PGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKW 182
           QK     NP ++FLL+G+ S EG KW+KHR+II+PAF  +KLK M+P      ++M+ KW
Sbjct: 128 QKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKW 187

Query: 183 ERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVY 242
           E++  + G  E+D+ P    L +DVIS+ AFGS+YEEG  IF+ LKEL DL +     VY
Sbjct: 188 EKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVY 247

Query: 243 IPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEI 302
           IPGW +LPTK N RMK+I+KEI  +L  ++NKR  ++KAG+    +DLL +LLESN +EI
Sbjct: 248 IPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEP-GEDDLLGVLLESNIQEI 307

Query: 303 EEHGS-NDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVF 362
           ++ G+  D GMSI D+IEECK+FYFAGQETT +LL WT +LLS +P+WQERAR EVL+ F
Sbjct: 308 QKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAF 367

Query: 363 GNNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIAL 422
           G NKP+F+ LN LK VSMIL EVLRLYPP    ++++ K+T+LG   +PAG  +  P  +
Sbjct: 368 GKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVM 427

Query: 423 IHRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSM 482
           +HR++ +WGEDA EFNP RF +GV+ AT+N   + PF WGPR+C+GQNFA+++AK+ L+M
Sbjct: 428 LHREKSIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAM 487

Query: 483 ILQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           ILQ FKF+++ SY HAP  + T+QPQ+G+H+I +KL
Sbjct: 488 ILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

[Close]

BLAST of Spg009069.1 vs. ExPASy TrEMBL
Match: A0A6J1EYN3 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111439752 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 7.6e-233
Identity = 381/513 (74.27%), Postives = 440/513 (85.77%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SIW V +V L+ WGW+FLNWIWL+PKKLE+ LR QGFAGNPYRL SGDMKD AAM E
Sbjct: 7   IRVSIWAVAVVCLAVWGWRFLNWIWLRPKKLERSLRNQGFAGNPYRLLSGDMKDIAAMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PSI+ TIHKYGK SFMW G YPRVH++DP+QLKAA+ L++D+
Sbjct: 67  QAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAALSLMHDV 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           QKP INP  R L  GL   EG KW KHRKIINPAFHF+ LKGMVP +CESC EMI KWE 
Sbjct: 127 QKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
           ++S+DG+ ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +LV+K TS VYIP
Sbjct: 187 VVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNNRMKKISKE++ +L  ++NKRQ +M AG  +H NDLLSILL+SN KEI E
Sbjct: 247 GWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMH-NDLLSILLQSNLKEIRE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           HG+ND+GMSIED+++EC+VFY AGQETTA+LL+WTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HGNNDVGMSIEDIVDECRVFYLAGQETTAMLLIWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLN LKIV+MILNEVLRLYPP  + SRL++ ET+LGK+  PAGVML FPI LIHR
Sbjct: 367 KPDFDGLNGLKIVTMILNEVLRLYPPAALVSRLVQNETKLGKLSFPAGVMLSFPIILIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D++LWG+DANEFNP RF EGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKKLWGDDANEFNPDRFTEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSYTHAPT +   +PQ+GAHI+LRKL
Sbjct: 487 HFSFELSSSYTHAPTSILITRPQHGAHIMLRKL 518

[Close]

BLAST of Spg009069.1 vs. ExPASy TrEMBL
Match: A0A6J1IHE1 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111473561 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 2.1e-230
Identity = 380/513 (74.07%), Postives = 438/513 (85.38%), Query Frame = 0


Query: 3   LKASIWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTE 62
           ++ SIW V +V L+ WGW+FLNWIWL+PKKLE+CLR+QGFAGNPYRL +GDMKD AAM E
Sbjct: 7   IRDSIWAVAVVCLAVWGWRFLNWIWLRPKKLERCLRKQGFAGNPYRLLTGDMKDIAAMRE 66

Query: 63  QAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDI 122
           QA S PMNFSHDI PRV PSI+ T HKYGK SFMW G YPRVH++DP+QLKAA  L++D+
Sbjct: 67  QAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAFSLMHDV 126

Query: 123 QKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWER 182
           QKP INP  R L  GL   EG KW KHRKIINPAFHF++LK MVP +CESC EMI KWE 
Sbjct: 127 QKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRKWEE 186

Query: 183 MISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIP 242
           ++S+D + ELDVMPHL NLAADVISRTAFGSSYEEGK+IF  LK+L +LV+K TS VYIP
Sbjct: 187 VVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTVYIP 246

Query: 243 GWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEE 302
           GWRF+PTKSNN MKKISKE+KT+L  ++NKRQ +M AG+ +H NDLLSILL+SN KEI E
Sbjct: 247 GWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAMH-NDLLSILLQSNLKEILE 306

Query: 303 HGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNN 362
           HG+ND+GMSIED+++EC+VFY AGQETTA+LLVWTMVLLSS  +WQ+ AR EVL+VFGNN
Sbjct: 307 HGNNDVGMSIEDIVDECRVFYLAGQETTAMLLVWTMVLLSSYTRWQDLAREEVLQVFGNN 366

Query: 363 KPDFDGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHR 422
           KPDFDGLNRLKIV+MILNEVLRLYPP  + SRL++ E +LGK+  PAGVML FPI LIHR
Sbjct: 367 KPDFDGLNRLKIVTMILNEVLRLYPPPALISRLVQNEIKLGKLSFPAGVMLSFPIILIHR 426

Query: 423 DQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQ 482
           D+ LWG+DANEFNP RFAEGVSKATQNQAGFFPFGWGPRICIGQNFA+IEAKIALSMILQ
Sbjct: 427 DKNLWGDDANEFNPDRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAIIEAKIALSMILQ 486

Query: 483 HFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           HF FELSSSY HAPT   T +PQ+GAHI+L KL
Sbjct: 487 HFSFELSSSYKHAPTSNLTTRPQHGAHIMLLKL 518

[Close]

BLAST of Spg009069.1 vs. ExPASy TrEMBL
Match: A0A5D3CEG3 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003160 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 3.0e-229
Identity = 383/517 (74.08%), Postives = 442/517 (85.49%), Query Frame = 0


Query: 1   MDLKAS-IWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAA 60
           MD + S IW V MV L R GW+FLNWIWL PKKLEKCLREQGFAGNPYRL+SGD+ D  A
Sbjct: 1   MDFRNSMIWFVFMVCLLRCGWRFLNWIWLTPKKLEKCLREQGFAGNPYRLYSGDLNDVVA 60

Query: 61  MTEQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLV 120
           M E+AKSKPMNFSHDIVPRV PS+H TI KYGK SFMW G  PRV ++DPEQLKAA+ L 
Sbjct: 61  MEEEAKSKPMNFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLY 120

Query: 121 YDIQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINK 180
            D QKP  NP +R L  GL++ EG+KW KHRKIIN AFHF+KLK MVPT+ ESCNEMI+K
Sbjct: 121 NDFQKPTTNPLVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISK 180

Query: 181 WERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRV 240
           WE+M S+DG CELDV+P+LQ+L ADVISRTAFGS+YEEGKIIF H KEL  LVL+ ++ V
Sbjct: 181 WEKMTSKDGSCELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTV 240

Query: 241 YIPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKE 300
           Y+PGWRFLPTKSNNRMKKI+KEIKT+L ++++KRQ +MK  +T+H++DLL ILLESN  E
Sbjct: 241 YLPGWRFLPTKSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSDDLLGILLESNSNE 300

Query: 301 IEEHGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVF 360
           I+EHG+ +IGMSI D+IEECK+FYFAGQETTA+LLVWTMVLL S  KWQ+RARAEVL+VF
Sbjct: 301 IKEHGNVNIGMSINDLIEECKIFYFAGQETTAVLLVWTMVLLGSYTKWQDRARAEVLQVF 360

Query: 361 GNNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSR-LIKKETRLGKVRLPAGVMLYFPIA 420
           GN+KPDFDGLNRLKIV+MILNEVLRLYPPG   SR L+KKETRLG ++  +GV L FPI 
Sbjct: 361 GNDKPDFDGLNRLKIVTMILNEVLRLYPPGSAVSRNLVKKETRLGNLKFSSGVTLSFPII 420

Query: 421 LIHRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALS 480
           LIHRD+ELWGEDA+EFNP+RFAEGVSKAT+NQ  FFPFGWGPRIC+GQNFAMIEAKIALS
Sbjct: 421 LIHRDKELWGEDADEFNPERFAEGVSKATKNQTAFFPFGWGPRICVGQNFAMIEAKIALS 480

Query: 481 MILQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           +ILQHF FELS SYTHAP  + TIQPQ+GAHIIL KL
Sbjct: 481 IILQHFSFELSPSYTHAPISILTIQPQHGAHIILHKL 517

[Close]

BLAST of Spg009069.1 vs. ExPASy TrEMBL
Match: A0A1S3BQL9 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103492242 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 3.0e-229
Identity = 383/517 (74.08%), Postives = 442/517 (85.49%), Query Frame = 0


Query: 1   MDLKAS-IWMVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAA 60
           MD + S IW V MV L R GW+FLNWIWL PKKLEKCLREQGFAGNPYRL+SGD+ D  A
Sbjct: 1   MDFRNSMIWFVFMVCLLRCGWRFLNWIWLTPKKLEKCLREQGFAGNPYRLYSGDLNDVVA 60

Query: 61  MTEQAKSKPMNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLV 120
           M E+AKSKPMNFSHDIVPRV PS+H TI KYGK SFMW G  PRV ++DPEQLKAA+ L 
Sbjct: 61  MEEEAKSKPMNFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLY 120

Query: 121 YDIQKPGINPFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINK 180
            D QKP  NP +R L  GL++ EG+KW KHRKIIN AFHF+KLK MVPT+ ESCNEMI+K
Sbjct: 121 NDFQKPTTNPLVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISK 180

Query: 181 WERMISEDGFCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRV 240
           WE+M S+DG CELDV+P+LQ+L ADVISRTAFGS+YEEGKIIF H KEL  LVL+ ++ V
Sbjct: 181 WEKMTSKDGSCELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTV 240

Query: 241 YIPGWRFLPTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKE 300
           Y+PGWRFLPTKSNNRMKKI+KEIKT+L ++++KRQ +MK  +T+H++DLL ILLESN  E
Sbjct: 241 YLPGWRFLPTKSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSDDLLGILLESNSNE 300

Query: 301 IEEHGSNDIGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVF 360
           I+EHG+ +IGMSI D+IEECK+FYFAGQETTA+LLVWTMVLL S  KWQ+RARAEVL+VF
Sbjct: 301 IKEHGNVNIGMSINDLIEECKIFYFAGQETTAVLLVWTMVLLGSYTKWQDRARAEVLQVF 360

Query: 361 GNNKPDFDGLNRLKIVSMILNEVLRLYPPGGMSSR-LIKKETRLGKVRLPAGVMLYFPIA 420
           GN+KPDFDGLNRLKIV+MILNEVLRLYPPG   SR L+KKETRLG ++  +GV L FPI 
Sbjct: 361 GNDKPDFDGLNRLKIVTMILNEVLRLYPPGSAVSRNLVKKETRLGNLKFSSGVTLSFPII 420

Query: 421 LIHRDQELWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALS 480
           LIHRD+ELWGEDA+EFNP+RFAEGVSKAT+NQ  FFPFGWGPRIC+GQNFAMIEAKIALS
Sbjct: 421 LIHRDKELWGEDADEFNPERFAEGVSKATKNQTAFFPFGWGPRICVGQNFAMIEAKIALS 480

Query: 481 MILQHFKFELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           +ILQHF FELS SYTHAP  + TIQPQ+GAHIIL KL
Sbjct: 481 IILQHFSFELSPSYTHAPISILTIQPQHGAHIILHKL 517

[Close]

BLAST of Spg009069.1 vs. ExPASy TrEMBL
Match: A0A5A7VAQ5 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00130 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 3.3e-204
Identity = 342/508 (67.32%), Postives = 411/508 (80.91%), Query Frame = 0


Query: 10  VVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPM 69
           V+++LL   GWK ++WIW +PKKLEK LR QGFAGN YR+  GD+K+SAAM E+A SKPM
Sbjct: 15  VLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILHGDLKESAAMREEAMSKPM 74

Query: 70  NFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINP 129
           NFS+ I PRV PS++ TI  YGKKS MW G  PRVH++DPEQLK     + D QKP +NP
Sbjct: 75  NFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQLKIVFSSINDFQKPTMNP 134

Query: 130 FLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESC-NEMINKWERMISEDG 189
             R L+ GL SLEGQKW KHRKIINPAFH +KLK MVP    SC NEM++KWE M+S +G
Sbjct: 135 LTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYHSCYNEMVSKWEGMVSREG 194

Query: 190 FCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLP 249
            CELDVMP+LQN+A+DVISRTAFGSSYE+GK IF+   EL +LV++    +YIPGWRFLP
Sbjct: 195 SCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELANLVIQANLGIYIPGWRFLP 254

Query: 250 TKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSN-D 309
           TKSN +MK+ISKEI T++  ++N+++ S+KAG+ +   DLLSIL+ESN  EI++HG+N +
Sbjct: 255 TKSNRKMKEISKEITTLILGIMNEKEKSIKAGEAI-QTDLLSILMESNMNEIKQHGNNKN 314

Query: 310 IGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFD 369
           IGMSIED+IEECK+FY AGQETTA LLVWTMVLLSS  +WQERARAEV E+FGN KPD+D
Sbjct: 315 IGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIFGNKKPDYD 374

Query: 370 GLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELW 429
           GLNRLK+V+MI NEVLRLYPP  M  R+I+KETRLG + LP GVML  P+ LIH D ELW
Sbjct: 375 GLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEGVMLGLPVVLIHLDPELW 434

Query: 430 GEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFE 489
           GEDA+EFNP+RF+EG+SKAT+N + F PFGWGPRICIGQNFAMIEAK+ALS+ILQ F FE
Sbjct: 435 GEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMIEAKMALSIILQRFSFE 494

Query: 490 LSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           LS SYTHAP  V T QPQ+GAHIIL KL
Sbjct: 495 LSPSYTHAPIAVLTTQPQHGAHIILHKL 521

[Close]

BLAST of Spg009069.1 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 575.5 bits (1482), Expect = 4.3e-164
Identity = 279/510 (54.71%), Postives = 367/510 (71.96%), Query Frame = 0


Query: 9   MVVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKP 68
           +VV+  ++ W WK LN  WL+PKK E  L+ QG +G P+    GD+K  A+M EQ KS+P
Sbjct: 15  VVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMVEQEKSRP 74

Query: 69  MNFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGIN 128
           +N + D   RV P I QT+  +GK S+MW G    V V  PE +K  +  VYD  KP ++
Sbjct: 75  INLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRVYDFPKPPVH 134

Query: 129 PFLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISEDG 188
           P +    +G+   EG+KW+KHRKIINP+FH +KLK M+P   ESC+EMI+KWE++++E G
Sbjct: 135 PIVELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISKWEKLVTEQG 194

Query: 189 FC-ELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFL 248
              E+DV P+L +L +DVISRTAFGSSYEEGK IF+  +E    VLK     +IPG RFL
Sbjct: 195 SSNEIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALELAFIPGMRFL 254

Query: 249 PTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSND 308
           PTK+N RM++I+KE+K+ L  ++ KRQ  M  G+    NDLL ILLESN        S D
Sbjct: 255 PTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGE-APKNDLLGILLESN--------SGD 314

Query: 309 IGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFG-NNKPDF 368
            GMSIED++EEC++F+FAGQETTA+LLVWTM++LS + KWQ++AR E+L+V G NNKP+F
Sbjct: 315 HGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKPNF 374

Query: 369 DGLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGK-VRLPAGVMLYFPIALIHRDQE 428
           D L+RLK +SMILNEVLRLYPPG +  R ++KET+LG+ + LP G  +  P+ ++HRD E
Sbjct: 375 DALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPE 434

Query: 429 LWGEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFK 488
           LWGED +EFNP+RFA+G+SKAT+NQ  F PFGWGPR C GQNFA++EAK+AL +ILQ F 
Sbjct: 435 LWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRFS 494

Query: 489 FELSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           FELS SYTHAP  V T+ PQ+GA +I   L
Sbjct: 495 FELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

[Close]

BLAST of Spg009069.1 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 567.4 bits (1461), Expect = 1.2e-161
Identity = 268/507 (52.86%), Postives = 359/507 (70.81%), Query Frame = 0


Query: 10  VVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPM 69
           VV+ ++S W W+ L W+W KPK LE  LR QG AG PY    GD+K +  M  +A+SKP+
Sbjct: 12  VVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSKPL 71

Query: 70  NFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINP 129
             + DI PRV P   Q    YG+  F WFG  P + ++DPEQ+K     VYD QKP   P
Sbjct: 72  KLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHTFP 131

Query: 130 FLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISEDG- 189
               +  GL + +G KWAKHR+IINPAFH +K+K MVP   +SC E++ +W++++S+ G 
Sbjct: 132 LATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDKGS 191

Query: 190 FCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLP 249
            CE+DV P L ++ ADVISRTAFGSSY+EG+ IF+   EL  L+++   + +IPG+ +LP
Sbjct: 192 SCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSYLP 251

Query: 250 TKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSNDI 309
           TKSN RMK  ++EI+ +L  +VNKR  + +AG+   ++DLL ILLESN ++ E +     
Sbjct: 252 TKSNRRMKAAAREIQVILRGIVNKRLRAREAGE-APSDDLLGILLESNLRQTEGN----- 311

Query: 310 GMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFDG 369
           GMS ED++EECK+FYFAGQETT++LLVWTMVLLS +  WQ RAR EV +VFG+ +PD +G
Sbjct: 312 GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEG 371

Query: 370 LNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELWG 429
           LN+LK+++MIL EVLRLYPP    +R I KE +LG + LP GV +  PI L+  D ELWG
Sbjct: 372 LNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIELWG 431

Query: 430 EDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFEL 489
            DA EFNP RF +G+SKAT++Q  FFPF WGPRICIGQNFA++EAK+A+++IL+ F FE+
Sbjct: 432 NDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSFEI 491

Query: 490 SSSYTHAPTFVFTIQPQYGAHIILRKL 516
           S SY HAP  V TI PQ+GA +I+ KL
Sbjct: 492 SPSYVHAPYTVITIHPQFGAQLIMHKL 512

[Close]

BLAST of Spg009069.1 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 565.5 bits (1456), Expect = 4.5e-161
Identity = 269/507 (53.06%), Postives = 362/507 (71.40%), Query Frame = 0


Query: 10  VVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPM 69
           V +V++S W W+ L W+W KPK LE  LR QG AG PY    GD+K + +M  +A+SKP+
Sbjct: 12  VAVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSKPI 71

Query: 70  NFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINP 129
           N + DI PR+ P   Q +  +G+  F WFGA P + ++DPEQ+   +  VYD QK    P
Sbjct: 72  NLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHTFP 131

Query: 130 FLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISE-DG 189
             R + +G++S +G KWAKHR+IINPAFH +K+K MVP   +SC+E++ KW++++S+ + 
Sbjct: 132 LGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDKES 191

Query: 190 FCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLP 249
            CE+DV P L ++ ADVISRTAFGSS  EG+ IF+   EL  L+++   + +IPG+ +LP
Sbjct: 192 SCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSYLP 251

Query: 250 TKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSNDI 309
           TK N RMK  ++EI+ +L  +VNKR  + +AG+   N+DLL ILLESN  + + +     
Sbjct: 252 TKGNRRMKAKAREIQVILRGIVNKRLRAREAGE-APNDDLLGILLESNLGQTKGN----- 311

Query: 310 GMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFDG 369
           GMS ED++EECK+FYF GQETT++LLVWTMVLLS +  WQ RAR EV +VFG+ +PD +G
Sbjct: 312 GMSTEDLMEECKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDAEG 371

Query: 370 LNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELWG 429
           LN+LK+++MIL EVLRLYPP    SR I KE  LG + LP GV++  PI L+ RD ELWG
Sbjct: 372 LNQLKVMTMILYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTELWG 431

Query: 430 EDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFEL 489
            DA EF P RF +G+SKAT+NQA FFPF WG RICIGQNFA++EAK+A+++ILQ F FEL
Sbjct: 432 NDAGEFKPDRFKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSFEL 491

Query: 490 SSSYTHAPTFVFTIQPQYGAHIILRKL 516
           S SY HAP  VFTI PQ+GA +I+ KL
Sbjct: 492 SPSYVHAPYTVFTIHPQFGAPLIMHKL 512

[Close]

BLAST of Spg009069.1 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 562.4 bits (1448), Expect = 3.8e-160
Identity = 263/508 (51.77%), Postives = 359/508 (70.67%), Query Frame = 0


Query: 10  VVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPM 69
           V++ ++  W W+ + W+W+KPK LE  L+ QG  G PY    GD+K +  M  +A+SKP+
Sbjct: 11  VLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSKPI 70

Query: 70  NFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINP 129
           N + DI PR+ P   + ++ +GK  F+W G  P + + +PEQ+K     V D +K    P
Sbjct: 71  NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKASTFP 130

Query: 130 FLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISE-DG 189
            +R L  GL S +G KWA HR+IINPAFH +K+K M+P     C+E++ +WE++ ++ + 
Sbjct: 131 LIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDKES 190

Query: 190 FCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFLP 249
             E+DV P L N+ ADVIS TAFGSSY+EG+ IFQ   EL +L+ +   + YIPG RF P
Sbjct: 191 PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGSRFYP 250

Query: 250 TKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSNDI 309
           TKSN RMK I +E+  +L  +V+KR+ + +AG+   N+DLL ILLESN +E     S   
Sbjct: 251 TKSNRRMKAIDREVDVILRGIVSKREKAREAGEPA-NDDLLGILLESNSEE-----SQGN 310

Query: 310 GMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFG-NNKPDFD 369
           GMS+ED+++ECK+FYFAGQETT++LLVWTMVLLS +  WQ RAR EV++V G NNKPD +
Sbjct: 311 GMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPDME 370

Query: 370 GLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELW 429
            LN LK+++MI NEVLRLYPP     R++ KE +LG++ LPAG+ +Y P  L+ RD ELW
Sbjct: 371 SLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTELW 430

Query: 430 GEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFE 489
           G+DA +F P+RF +G+SKAT+NQ  FFPFGWGPRICIGQNFAM+EAK+A+++ILQ F FE
Sbjct: 431 GDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFSFE 490

Query: 490 LSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           LS SY HAP  V T +PQ+GAH+IL KL
Sbjct: 491 LSPSYVHAPQTVMTTRPQFGAHLILHKL 512

[Close]

BLAST of Spg009069.1 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 557.8 bits (1436), Expect = 9.4e-159
Identity = 268/508 (52.76%), Postives = 358/508 (70.47%), Query Frame = 0


Query: 10  VVMVLLSRWGWKFLNWIWLKPKKLEKCLREQGFAGNPYRLFSGDMKDSAAMTEQAKSKPM 69
           VV+V++S W W+ L W+W KPK LE  LR QG AG PY    GD+K +  M  +A SKP+
Sbjct: 12  VVVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLKRNVNMLTEATSKPI 71

Query: 70  NFSHDIVPRVFPSIHQTIHKYGKKSFMWFGAYPRVHVVDPEQLKAAVCLVYDIQKPGINP 129
             + DI PRV P   Q +  +G+  F W G  P + ++DPE +K     VYD  K     
Sbjct: 72  KLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEVFNKVYDYPKAQTFL 131

Query: 130 FLRFLLSGLVSLEGQKWAKHRKIINPAFHFQKLKGMVPTICESCNEMINKWERMISEDG- 189
             R + +G+++ +G KWAKHR+IINPAFH +K+K MVP   +SC++++ +W +++S+ G 
Sbjct: 132 LGRLIATGIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSDVVGEWSKLVSDKGS 191

Query: 190 -FCELDVMPHLQNLAADVISRTAFGSSYEEGKIIFQHLKELTDLVLKVTSRVYIPGWRFL 249
             CE+DV P L ++  DVISRTAFGSSY+EG+ IF+   EL  L+L+   +VYIPG+R+L
Sbjct: 192 SSCEVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLILQAFWKVYIPGYRYL 251

Query: 250 PTKSNNRMKKISKEIKTVLFSLVNKRQMSMKAGDTVHNNDLLSILLESNFKEIEEHGSND 309
           PTKSN RMK  ++EI+ +L  +VNKR  + +AG    N+DLL ILLESN  + + +    
Sbjct: 252 PTKSNRRMKAAAREIQVILKGIVNKRLRAREAGKAAPNDDLLGILLESNLGQAKGN---- 311

Query: 310 IGMSIEDMIEECKVFYFAGQETTALLLVWTMVLLSSNPKWQERARAEVLEVFGNNKPDFD 369
            GMS ED++EECK+FYFAGQETT++LLVW MVLLS +  WQ RAR EV +VFG+ +PD +
Sbjct: 312 -GMSTEDVMEECKLFYFAGQETTSVLLVWAMVLLSHHQDWQARAREEVKQVFGDKEPDTE 371

Query: 370 GLNRLKIVSMILNEVLRLYPPGGMSSRLIKKETRLGKVRLPAGVMLYFPIALIHRDQELW 429
            L++LK+++MIL EVLRLYPP    +R I KE +LG + LPAGV +  PI L+ RD  LW
Sbjct: 372 CLSQLKVMTMILYEVLRLYPPVTHLTRAIDKEMKLGDLTLPAGVHISLPIMLVQRDPMLW 431

Query: 430 GEDANEFNPQRFAEGVSKATQNQAGFFPFGWGPRICIGQNFAMIEAKIALSMILQHFKFE 489
           G DA EF P+RF +G+SKAT++Q  FFPF WGPRICIGQNFAM+EAK+A+++ILQ F FE
Sbjct: 432 GTDAAEFKPERFKDGLSKATKSQVSFFPFAWGPRICIGQNFAMLEAKMAMALILQTFTFE 491

Query: 490 LSSSYTHAPTFVFTIQPQYGAHIILRKL 516
           LS SY HAP  V TI PQ+GAH+ILRKL
Sbjct: 492 LSPSYVHAPQTVVTIHPQFGAHLILRKL 514

The following BLAST results are available for this feature:

BLAST of Spg009069.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878667.1	3.3e-238	76.74	cytochrome P450 CYP72A219-like isoform X1 [Benincasa hispida]	[more]
XP_022933047.1	1.6e-232	74.27	cytochrome P450 CYP72A219-like [Cucurbita moschata]	[more]
XP_022974803.1	4.3e-230	74.07	cytochrome P450 CYP72A219-like [Cucurbita maxima]	[more]
XP_023516285.1	7.3e-230	73.49	cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_023520477.1	9.5e-230	73.88	cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg009069.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH21	3.2e-180	56.50	Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
A0A0S2IHL2	5.5e-180	58.01	Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1	[more]
Q2MJ19	3.7e-168	53.59	Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1	[more]
W8JWW3	7.7e-166	54.29	Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1	[more]
Q05047	6.5e-165	51.74	Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1	[more]

BLAST of Spg009069.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EYN3	7.6e-233	74.27	cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111439752 PE=...	[more]
A0A6J1IHE1	2.1e-230	74.07	cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111473561 PE=3 ...	[more]
A0A5D3CEG3	3.0e-229	74.08	Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BQL9	3.0e-229	74.08	cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103492242 PE=3 SV=1	[more]
A0A5A7VAQ5	3.3e-204	67.32	Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Spg009069.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14620.1	4.3e-164	54.71	cytochrome P450, family 72, subfamily A, polypeptide 8	[more]
AT3G14690.1	1.2e-161	52.86	cytochrome P450, family 72, subfamily A, polypeptide 15	[more]
AT3G14650.1	4.5e-161	53.06	cytochrome P450, family 72, subfamily A, polypeptide 11	[more]
AT3G14610.1	3.8e-160	51.77	cytochrome P450, family 72, subfamily A, polypeptide 7	[more]
AT3G14640.1	9.4e-159	52.76	cytochrome P450, family 72, subfamily A, polypeptide 10	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 325..342 score: 38.63 coord: 454..463 score: 57.77 coord: 377..388 score: 40.16 coord: 463..474 score: 40.73
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 84..490 e-value: 1.7E-81 score: 274.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 201..219 score: 23.49 coord: 334..360 score: 29.71 coord: 453..463 score: 53.49 coord: 463..486 score: 32.47 coord: 87..106 score: 24.83 coord: 314..331 score: 25.46
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 71..514 e-value: 1.3E-107 score: 362.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 76..513
None	No IPR available	PANTHER	PTHR24282:SF140	CYTOCHROME	coord: 7..512
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 7..512
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 456..465

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg009069	Spg009069	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg009069.1-cds	Spg009069.1-cds-scaffold2:28016628..28017053	CDS
Spg009069.1-cds	Spg009069.1-cds-scaffold2:28018234..28018621	CDS
Spg009069.1-cds	Spg009069.1-cds-scaffold2:28019645..28019889	CDS
Spg009069.1-cds	Spg009069.1-cds-scaffold2:28021084..28021301	CDS
Spg009069.1-cds	Spg009069.1-cds-scaffold2:28022618..28022888	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg009069.1-five_prime_utr	Spg009069.1-five_prime_utr-scaffold2:28022889..28022945	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg009069.1	Spg009069.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen