Spg005823.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg005823.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Omega-3 fatty acid desaturase
Location	scaffold11: 930946 .. 934972 (+)
Sequence length	1134
RNA-Seq Expression	Spg005823.1
Synteny	Spg005823.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAACACAAAATTAAAAATTCACATACTCGATACAAAATTAAAAATTTAAGAACCTATCGAGTATCAGATTTTCACTAGACGTAAGAGTAAAATTTCAAAAATCGGTAGACACTCTTTAAAGTCGAGTGACCAAATTTAGTCGTTAAACTTATAATTTAACCTAAATAAAATAAAAAAAAAATAAAAAGTCAAAATAAGAGGTGTAGCGATGGGTTCGTAAGCCAAGAGTTCCCTTTTATAATCACTTCCTTCCCTCATTCTTCATTTGCCCTTTTCCCCTTCAATCCCCAACCCTCCAAAATTCCTCCCCAATCCACCATGGCCATTCCTCTGTCGCCCTCCGCCACCTGCAACGGCAACGGCAACGCCACCGGCACGGCGGCGCCTCCCCCTTTCGACCCCTCCGCCCCTCCCCCCTTCCGCATCTCCGAGATCCGCGCCGCCATTCCCCCCCATTGCTGGGTCAAGAACCCCTGGCGCTCCCTCGTCTACGTCCTCCGCGATTTCGCCGTCGTCTCCGTATTGGTCGCCGCCGCCGTCTCCCTCGACTCCTGGCTCGTCTGGCCCCTCTACTGGCTCGCTCAGGGCACCATGTTCTGGGCCATCTTCGTCCTCGGCCACGACTGGTTTGCTTCCATTCTCAACTCCCTCTGCTCTGTTTTCCTCTCCGATTCTCATCTCTCTGTTTTTTTACAGCGGCCATGGCAGCTTCTCCAACAGCTCCGCCCTCAATTCCTTCATGGGTCTCCTTCTTCACTCCGCCATTTTGGTTCCTTACCATGGATGGTACTTTCTTCTTCCCCCAAATCCCCAGATCGATCTTCCTCTGTTCTTCCGACATTGAAACCAGAGGCACTCTGTTTTTTTGTTCTTGCAGGAGAATCAGCCACAGAACCCATCATCAGAACCATGGAAATGTGGAGAAAGACGAATCCTGGGTTCCAGTACGTTCGTCAGATTTGTCGGATCTCTCTGTTTTTTAAGGTTTTACATCCATTTCAATGGCGGTTTTTTGTTTTTTTCAGATGACGAAGAAGACTTACAAGCAGCTGGAGAGAAGAACACGAATCCTCAGATTCACTCTGCCTTTCCCCATTCTGGCTTACCCGTTTTACCTGGTGAGTGATTTCGGTTTCGATTCGGTTCGGTTCGATTCGGTTTCGGATATCGAAAATTTGTGTTTGTTGTGTTGCAGATGTGGAGAAGCCCAGGAAAAGAAGGATCTCATTTCAATCCATACAGCGAATTGTTTGCTCCAAATGAGAGAAAAGACATTGTGATTTCGACCTCGTGTTGGACATTAATGGCGGTTCTTTTAGTCTATTTGTCCTTCGTTTTTGGTCCCATTCAAATGTTCAAAATCTATGGCGTTCCTTACTGGGTGAAGATCTCTCCTTTTCTCTTATGTTCTTTTTCTTTTTAGCCGAAATTAAGGAAGAAAAGTTTGGTCGATTTTCAAATTTTTAAGGAATGAATGAATTGATTTGATTCAATGGTATTAATGATGGTTGCTGGTCAACATCTCTGCATTTTTCTTTTCACATTAAAAGAAATTCCATTTCTAAGTTTCTCTCACATGGCACAACTCATCAGTTTCATTCAAAAAAGTTGAAAAAGTTTCCATTTTTTTATATTTTACATCATGGGTTTTTGTTTTTGGATCTGAATTTGAATTGGGTTTTTCTTTTTTTTAGATTTTTGTGATGTGGTTGGACTTTGTGACATATCTGCACCACCATGGGTATGAACAGAAGCTGCCTTGGTACAGAGGAGAGGTAAGAAAAGAGATAAAGATTTTTCCTTTTTTTTTGGGAAAGAGAATTTTTTTTTGAAACCCAGATGGGAAAATTTAAAAAAAAAAAAAAAAAATTGATGGACAGGAATGGACTTACCTACGTGGCGGGCTGACGACCGTTGATCGGGATTATGGCTTGTTCAACAACATCCATCATGATATCGGAACTCATGTTATCCATCATTTGTTTCCTCAGATCCCTCATTATCATCTCGTGGAAGCGGTATGTCTTGATCGTTGAATTATGTTTTGAATTATGTTTTGAATTATGTTTTGAATTATGTTTTGAATTAGCGTGTTCTTATTATAAGATAGTTGTTAATCTAAAAAGGGAAAAAAATGGTTTTAGGACGGGATAGGAATGAAATAGTCATATTTAGAGTATGTTTGGTTTAACTGACGAGATTGAGAAATAAACTATTTTGAGAAAAACACCTAGAAAACAAATTTTTAAAAACATTTATGAGAAAAAGTAATCAAACGGATGAGTGTTTAAATTCTAAACATTTTTTATACAAAATGTTTAAAATAAAATGCATTTTTAAGATATTTCAAACGTTTCAAATCTTAATTTCAAACTTTTTTTTTATAGGAATATTCCAAACTTGTTTTAATTTTGTAAACTAAGATGTTGGAAATGTTTTGTTGTAATAGAATATGTAACGGCATTAATTAGTTCAATAAGCATGAATTTTATAAAACAGAAAAGGACAAAAAAATTTGTCTTCTTTTTCAGTTTTAAAAAATTGAAATTGTTTTATTAAACAATTTCCACTACCTAACTTTCACTTTTTAAAGTTTCAAAAAAAAAAAAAAACTTTCACTTTCTAAAAACCAAACCCTTTTTCGAAAGGCTAAATTATAAAAAATGTCCCTGAACTATGGAGTTTGTTTAAAAAATGCCCCTAAACATTGAAAAGTTTCAAAAATACTCATGAACTTTGAAAAATGTTTCAAAAATACCCTTGTCGTTAATTTTTGGATGGAAACCGTTAAAGTTTCAGTTTCAAAAATACCTTTGAACTTTCAAAAAAAATTAAAAAATACTCTTATTAAAAAATGCTTCTTCTTCTTTCCTCCTCTCACCATAAATTCTAACAGTTTTCATCCAAAAATTAACTACAAGAGTATTTTTGAAACATTTTTCAAAGTTCAGGGGTATTTTTTTATAATTTAGCCTTTTTTAAATCTTTAACAAATACCCAAACAATTTTTTTAGAAATCACCAATCCCTTTTAGCTTTTTATACTTTTCCCATAATTTTATTTTCAAATCCCTTTTGGAAAAAAAAAAAAGTAAGTTATTAATCCTTTTGTTTTTTCTCTATTTTTAGAGATCATAAATTAAGTCAAATTTTGAAAAACTTTATAAAATAACCCTCTTTAGATCATAATCAAGTCAAAATTTGGAAAACTTTAAAAAAGAACTAATTTTAATATATTCTTTTAAAATTGGATTAAGAATTCAAATCTATTTATAATAGAAATAGAAAGCATACCTAAGAAATTGTGAGGAAACTACCATAATTCTAAAAAAATAGAATCATTGTCTAATAGGGTATAAACAAGTAGAACTTTCAAACCCAAAGTCATCATCAAACATAACCCTGGAACCAAATTTTTCGATCCAAATCCAACTTGACCTCTTTTAAGACAGAAAACAACACCCCCATTAACAAAAGATAAAAGTAAAAAGGCACAAATTTTACATCAACCCCTACAAACCATTTGTATAGAAGTCGGCACACTAAAGATTCAAACTTCAATGGCAACCAACGAAGACTCCAAAAGGCAGGTGAAATGCTTTGTCGAGTAAGATCAGTCGAAACATACCTAAATCGACCCGAACCCATATTCAAAAGGAATGGGAAAGGACCCAATAGTAATAGAGTAAAGTTAGTGGAGGAGGAAGGAAAATGGCAATAATTGAATTGAAATGTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAGACAAAGGCAGCAAAGGGAGTGTTGGGGAAGTACTATAGAGAGCCAAAGAAATCAGGACCAATTCCATTTCATTTGGTAAAGAATCTGGTGAATAGCCTAAGACAAGACCACTATGTCAGTGATCAGGGCAATATTGTCTTTTACCAGACAGATCCTTATCTTTACCACTAAAAATTCATTTCTCTTCTCCTTTTTTTTTCAAAGCTTAGTCAGTTTTTCTTTTACAATTGTTCTTCTTGTTGCCTTAGTTGCTCTTGTA

mRNA sequence

ATGGCCATTCCTCTGTCGCCCTCCGCCACCTGCAACGGCAACGGCAACGCCACCGGCACGGCGGCGCCTCCCCCTTTCGACCCCTCCGCCCCTCCCCCCTTCCGCATCTCCGAGATCCGCGCCGCCATTCCCCCCCATTGCTGGGTCAAGAACCCCTGGCGCTCCCTCGTCTACGTCCTCCGCGATTTCGCCGTCGTCTCCGTATTGGTCGCCGCCGCCGTCTCCCTCGACTCCTGGCTCGTCTGGCCCCTCTACTGGCTCGCTCAGGGCACCATGTTCTGGGCCATCTTCGTCCTCGGCCACGACTGCGGCCATGGCAGCTTCTCCAACAGCTCCGCCCTCAATTCCTTCATGGGTCTCCTTCTTCACTCCGCCATTTTGGTTCCTTACCATGGATGGAGAATCAGCCACAGAACCCATCATCAGAACCATGGAAATGTGGAGAAAGACGAATCCTGGGTTCCAATGACGAAGAAGACTTACAAGCAGCTGGAGAGAAGAACACGAATCCTCAGATTCACTCTGCCTTTCCCCATTCTGGCTTACCCGTTTTACCTGATGTGGAGAAGCCCAGGAAAAGAAGGATCTCATTTCAATCCATACAGCGAATTGTTTGCTCCAAATGAGAGAAAAGACATTGTGATTTCGACCTCGTGTTGGACATTAATGGCGGTTCTTTTAGTCTATTTGTCCTTCGTTTTTGGTCCCATTCAAATGTTCAAAATCTATGGCGTTCCTTACTGGATTTTTGTGATGTGGTTGGACTTTGTGACATATCTGCACCACCATGGGTATGAACAGAAGCTGCCTTGGTACAGAGGAGAGGAATGGACTTACCTACGTGGCGGGCTGACGACCGTTGATCGGGATTATGGCTTGTTCAACAACATCCATCATGATATCGGAACTCATGTTATCCATCATTTGTTTCCTCAGATCCCTCATTATCATCTCGTGGAAGCGACAAAGGCAGCAAAGGGAGTGTTGGGGAAGTACTATAGAGAGCCAAAGAAATCAGGACCAATTCCATTTCATTTGGTAAAGAATCTGGTGAATAGCCTAAGACAAGACCACTATGTCAGTGATCAGGGCAATATTGTCTTTTACCAGACAGATCCTTATCTTTACCACTAA

Coding sequence (CDS)

ATGGCCATTCCTCTGTCGCCCTCCGCCACCTGCAACGGCAACGGCAACGCCACCGGCACGGCGGCGCCTCCCCCTTTCGACCCCTCCGCCCCTCCCCCCTTCCGCATCTCCGAGATCCGCGCCGCCATTCCCCCCCATTGCTGGGTCAAGAACCCCTGGCGCTCCCTCGTCTACGTCCTCCGCGATTTCGCCGTCGTCTCCGTATTGGTCGCCGCCGCCGTCTCCCTCGACTCCTGGCTCGTCTGGCCCCTCTACTGGCTCGCTCAGGGCACCATGTTCTGGGCCATCTTCGTCCTCGGCCACGACTGCGGCCATGGCAGCTTCTCCAACAGCTCCGCCCTCAATTCCTTCATGGGTCTCCTTCTTCACTCCGCCATTTTGGTTCCTTACCATGGATGGAGAATCAGCCACAGAACCCATCATCAGAACCATGGAAATGTGGAGAAAGACGAATCCTGGGTTCCAATGACGAAGAAGACTTACAAGCAGCTGGAGAGAAGAACACGAATCCTCAGATTCACTCTGCCTTTCCCCATTCTGGCTTACCCGTTTTACCTGATGTGGAGAAGCCCAGGAAAAGAAGGATCTCATTTCAATCCATACAGCGAATTGTTTGCTCCAAATGAGAGAAAAGACATTGTGATTTCGACCTCGTGTTGGACATTAATGGCGGTTCTTTTAGTCTATTTGTCCTTCGTTTTTGGTCCCATTCAAATGTTCAAAATCTATGGCGTTCCTTACTGGATTTTTGTGATGTGGTTGGACTTTGTGACATATCTGCACCACCATGGGTATGAACAGAAGCTGCCTTGGTACAGAGGAGAGGAATGGACTTACCTACGTGGCGGGCTGACGACCGTTGATCGGGATTATGGCTTGTTCAACAACATCCATCATGATATCGGAACTCATGTTATCCATCATTTGTTTCCTCAGATCCCTCATTATCATCTCGTGGAAGCGACAAAGGCAGCAAAGGGAGTGTTGGGGAAGTACTATAGAGAGCCAAAGAAATCAGGACCAATTCCATTTCATTTGGTAAAGAATCTGGTGAATAGCCTAAGACAAGACCACTATGTCAGTGATCAGGGCAATATTGTCTTTTACCAGACAGATCCTTATCTTTACCACTAA

Protein sequence

MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLYH

Homology

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BLAST of Spg005823.1 vs. NCBI nr
Match: KAA0031835.1 (acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo var. makuwa])

HSP 1 Score: 743.0 bits (1917), Expect = 1.2e-210
Identity = 350/377 (92.84%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  +   NGNGNAT  AAP PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSLVYVL
Sbjct: 1   MAIPLPANGNGNGNGNAT-AAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RD AVVS LVAAAV  DSWL WP+YWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL
Sbjct: 61  RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           LLHS ILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRFTLPFPIL
Sbjct: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS+LFAP+ERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD
Sbjct: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSL+QDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360

Query: 361 SDQGNIVFYQTDPYLYH 378
           SDQGNIVFYQTDPYLYH
Sbjct: 361 SDQGNIVFYQTDPYLYH 376

[Close]

BLAST of Spg005823.1 vs. NCBI nr
Match: XP_008457364.1 (PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis melo])

HSP 1 Score: 738.4 bits (1905), Expect = 3.0e-209
Identity = 349/378 (92.33%), Postives = 358/378 (94.71%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPP-PFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYV 60
           MAIPL      NGNGN+  TAA P PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSLVYV
Sbjct: 1   MAIPL----PANGNGNSNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYV 60

Query: 61  LRDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG 120
           LRD AVVS LVAAAV  DSWL WP+YWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG
Sbjct: 61  LRDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG 120

Query: 121 LLLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPI 180
           LLLHS ILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRFTLPFPI
Sbjct: 121 LLLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPI 180

Query: 181 LAYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQM 240
           LAYPFYLMWRSPGKEGSHFNPYS+LFAP+ERKDIVISTSCWTLMAVLLVYLSFVFGP Q+
Sbjct: 181 LAYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQI 240

Query: 241 FKIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH 300
           FK+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH
Sbjct: 241 FKLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH 300

Query: 301 DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHY 360
           DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSL+QDHY
Sbjct: 301 DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHY 360

Query: 361 VSDQGNIVFYQTDPYLYH 378
           VSDQGNIVFYQTDPYLYH
Sbjct: 361 VSDQGNIVFYQTDPYLYH 374

[Close]

BLAST of Spg005823.1 vs. NCBI nr
Match: XP_022997807.1 (omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita maxima])

HSP 1 Score: 731.9 bits (1888), Expect = 2.8e-207
Identity = 339/376 (90.16%), Postives = 355/376 (94.41%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  S   N    A GTAAPP FDPSAPPPFRISEIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1   MAIPLPASRPGNDTVYANGTAAPPSFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RDF ++S LVAAA  LDSWLVWP+YW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61  RDFVIISALVAAAAYLDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHSAILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRF+LPFPI 
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS++FAPNERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPTQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFV+WLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWIFVIWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLINNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNS RQDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD+GN+VFYQTDPYLY
Sbjct: 361 SDEGNVVFYQTDPYLY 376

[Close]

BLAST of Spg005823.1 vs. NCBI nr
Match: XP_023523732.1 (omega-3 fatty acid desaturase, endoplasmic reticulum-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.5 bits (1887), Expect = 3.7e-207
Identity = 337/376 (89.63%), Postives = 355/376 (94.41%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  S   N   +  GTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1   MAIPLPASRPGNDTVHTNGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RDF ++S LVAAA   DSWLVWP+YW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61  RDFVIISALVAAAAYFDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHSAILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRF+LPFPI 
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS++FAPNERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPTQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFV+WLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWIFVIWLDVVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLINNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNS RQDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD+GN+VFYQTDPYLY
Sbjct: 361 SDEGNVVFYQTDPYLY 376

[Close]

BLAST of Spg005823.1 vs. NCBI nr
Match: XP_023523733.1 (omega-3 fatty acid desaturase, endoplasmic reticulum-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.1 bits (1886), Expect = 4.9e-207
Identity = 337/376 (89.63%), Postives = 355/376 (94.41%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  S   N   +  GTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1   MAIPLPASRPGNDTVHTNGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RDF ++S LVAAA   DSWLVWP+YW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61  RDFIIISALVAAAAYFDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHSAILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRF+LPFPI 
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS++FAPNERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPTQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFV+WLD VTYLHHHGYEQKLPWYRGEEW+YLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWIFVIWLDVVTYLHHHGYEQKLPWYRGEEWSYLRGGLTTVDRDYGLINNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNS RQDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD+GN+VFYQTDPYLY
Sbjct: 361 SDEGNVVFYQTDPYLY 376

[Close]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot
Match: P48626 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=4097 GN=FAD3 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.8e-167
Identity = 268/351 (76.35%), Postives = 303/351 (86.32%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDPSAPPPFR++EIR  IP HCWVK+P RSL YV+RD   V+ L+  A+ LDSWL +PLY
Sbjct: 22  FDPSAPPPFRLAEIRNVIPKHCWVKDPLRSLSYVVRDVIFVATLIGIAIHLDSWLFYPLY 81

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           W  QGTMFWAIFVLGHDCGHGSFS+S  LN+ +G +LHSAILVPYHGWRISH+THHQNHG
Sbjct: 82  WAIQGTMFWAIFVLGHDCGHGSFSDSQLLNNVVGHILHSAILVPYHGWRISHKTHHQNHG 141

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           NVE DESWVPM +K Y ++   T+ LR+ +PFP+LAYP YLM RSPGK GSHFNPYS+LF
Sbjct: 142 NVETDESWVPMPEKLYNKVGYSTKFLRYKIPFPLLAYPMYLMKRSPGKSGSHFNPYSDLF 201

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P+ERK +V ST CWT+MA LL+YL   FG +QMFKIYG PY IFVMWLDFVTYLHHHGY
Sbjct: 202 QPHERKYVVTSTLCWTVMAALLLYLCTAFGSLQMFKIYGAPYLIFVMWLDFVTYLHHHGY 261

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
           E+KLPWYRG+EW+YLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHL EATKAA
Sbjct: 262 EKKLPWYRGKEWSYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLREATKAA 321

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLY 377
           K VLGKYYREPKKSGPIPFHLVK+L  S++QDHYVSD G IVFYQTDP+++
Sbjct: 322 KPVLGKYYREPKKSGPIPFHLVKDLTRSMKQDHYVSDSGEIVFYQTDPHIF 372

[Close]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot
Match: P48625 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=FAD3 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 8.2e-165
Identity = 258/352 (73.30%), Postives = 302/352 (85.80%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDPSAPPPF+I+EIRA+IP HCWVKNPWRSL YVLRD  V++ LVAAA+  D+WL+W +Y
Sbjct: 25  FDPSAPPPFKIAEIRASIPKHCWVKNPWRSLSYVLRDVLVIAALVAAAIHFDNWLLWLIY 84

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
              QGTMFWA+FVLGHDCGHGSFS+S  LNS +G +LHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 85  CPIQGTMFWALFVLGHDCGHGSFSDSPLLNSLVGHILHSSILVPYHGWRISHRTHHQNHG 144

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           ++EKDESWVP+T+K YK L+  TR++RFT+PFP+  YP YL  RSPGKEGSHFNPYS LF
Sbjct: 145 HIEKDESWVPLTEKIYKNLDSMTRLIRFTVPFPLFVYPIYLFSRSPGKEGSHFNPYSNLF 204

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P+ERK I IST CW  M  LL+YLSF+  P+ + K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 205 PPSERKGIAISTLCWATMFSLLIYLSFITSPLLVLKLYGIPYWIFVMWLDFVTYLHHHGH 264

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
            QKLPWYRG+EW+YLRGGLTTVDRDYG   NIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 265 HQKLPWYRGKEWSYLRGGLTTVDRDYGWIYNIHHDIGTHVIHHLFPQIPHYHLVEATQAA 324

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLYH 378
           K VLG YYREP++S P+PFHL+K L+ S+RQDH+VSD G++V+YQTD  L H
Sbjct: 325 KPVLGDYYREPERSAPLPFHLIKYLIQSMRQDHFVSDTGDVVYYQTDSLLLH 376

[Close]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot
Match: P32291 (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Vigna radiata var. radiata OX=3916 GN=ARG1 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-164
Identity = 267/364 (73.35%), Postives = 307/364 (84.34%), Query Frame = 0


Query: 13  GNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAA 72
           GNG   G  +   FDP APPPF+I++IRAAIP HCW K+  RSL YVLRD  VV+ L A+
Sbjct: 11  GNGAREGDQS--YFDPGAPPPFKIADIRAAIPKHCWEKSTLRSLSYVLRDVLVVTALAAS 70

Query: 73  AVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHG 132
           A+S +SW  WPLYW AQGTMFWA+FVLGHDCGHGSFSNSS LNSF+G +LHS ILVPY+G
Sbjct: 71  AISFNSWFFWPLYWPAQGTMFWALFVLGHDCGHGSFSNSSKLNSFVGHILHSLILVPYNG 130

Query: 133 WRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPG 192
           WRISHRTHHQNHG+VEKDESWVP+T+K YK L+  TR+LR++ PFPI AYPFYL  RSPG
Sbjct: 131 WRISHRTHHQNHGHVEKDESWVPLTEKVYKNLDDMTRMLRYSFPFPIFAYPFYLWNRSPG 190

Query: 193 KEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVM 252
           KEGSHFNPYS LF+P ERK +V ST CW ++  +L+YLS   GPI M K+YGVPY IFVM
Sbjct: 191 KEGSHFNPYSNLFSPGERKGVVTSTLCWGIVLSVLLYLSLTIGPIFMLKLYGVPYLIFVM 250

Query: 253 WLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQ 312
           WLDFVTYLHHHGY  KLPWYRG+EW+YLRGGLTTVDRDYG  NN+HHDIGTHVIHHLFPQ
Sbjct: 251 WLDFVTYLHHHGYTHKLPWYRGQEWSYLRGGLTTVDRDYGWINNVHHDIGTHVIHHLFPQ 310

Query: 313 IPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTD 372
           IPHYHLVEATK+AK VLGKYYREP+KSGP+PFHL+K L+ S+ QDH+VSD G+IV+YQTD
Sbjct: 311 IPHYHLVEATKSAKSVLGKYYREPQKSGPLPFHLLKYLLQSISQDHFVSDTGDIVYYQTD 370

Query: 373 PYLY 377
           P L+
Sbjct: 371 PKLH 372

[Close]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot
Match: P46310 (sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD7 PE=1 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 1.8e-164
Identity = 258/351 (73.50%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDP APPPF +++IRAAIP HCWVKNPW+SL YV+RD A+V  L A A  L++W+VWPLY
Sbjct: 88  FDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLY 147

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           WLAQGTMFWA+FVLGHDCGHGSFSN   LNS +G LLHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 148 WLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHG 207

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           +VE DESW PM++K Y  L++ TR  RFTLP  +LAYPFYL  RSPGK+GSH++P S+LF
Sbjct: 208 HVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLF 267

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P ERKD++ ST+CWT MA LLV L+F  GPIQM K+YG+PYWI VMWLDFVTYLHHHG+
Sbjct: 268 LPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGH 327

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
           E KLPWYRG+EW+YLRGGLTT+DRDYGL NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 328 EDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 387

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLY 377
           K VLGKYYREP KSGP+P HL++ L  S+++DHYVSD+G +V+Y+ DP LY
Sbjct: 388 KPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGEVVYYKADPNLY 438

[Close]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot
Match: P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 9.0e-164
Identity = 255/350 (72.86%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDP APPPF +++IRAAIP HCWVKNPW S+ YV+RD A+V  L A A   ++WL+WPLY
Sbjct: 81  FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           W AQGTMFWA+FVLGHDCGHGSFSN   LNS  G LLHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           +VE DESW P+ +  YK LE+ T++ RFTLPFP+LAYPFYL  RSPGK+GSH++P S+LF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P E+KD++ ST+CWT MA LLV L+FV GPIQM K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
           E KLPWYRG+EW+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYL 376
           K VLGKYYREPK SGP+P HL+ +L+ S++QDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430

[Close]

BLAST of Spg005823.1 vs. ExPASy TrEMBL
Match: A0A5A7SRR2 (Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00850 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.0e-211
Identity = 350/377 (92.84%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  +   NGNGNAT  AAP PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSLVYVL
Sbjct: 1   MAIPLPANGNGNGNGNAT-AAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RD AVVS LVAAAV  DSWL WP+YWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL
Sbjct: 61  RDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           LLHS ILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRFTLPFPIL
Sbjct: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPIL 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS+LFAP+ERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD
Sbjct: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSL+QDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHYV 360

Query: 361 SDQGNIVFYQTDPYLYH 378
           SDQGNIVFYQTDPYLYH
Sbjct: 361 SDQGNIVFYQTDPYLYH 376

[Close]

BLAST of Spg005823.1 vs. ExPASy TrEMBL
Match: A0A1S3C608 (acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis melo OX=3656 GN=LOC103497071 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.5e-209
Identity = 349/378 (92.33%), Postives = 358/378 (94.71%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPP-PFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYV 60
           MAIPL      NGNGN+  TAA P PFDPSAPPPFRI+EIRAAIPPHCWVKNPWRSLVYV
Sbjct: 1   MAIPL----PANGNGNSNATAAAPVPFDPSAPPPFRIAEIRAAIPPHCWVKNPWRSLVYV 60

Query: 61  LRDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG 120
           LRD AVVS LVAAAV  DSWL WP+YWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG
Sbjct: 61  LRDLAVVSALVAAAVFFDSWLFWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMG 120

Query: 121 LLLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPI 180
           LLLHS ILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRFTLPFPI
Sbjct: 121 LLLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFTLPFPI 180

Query: 181 LAYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQM 240
           LAYPFYLMWRSPGKEGSHFNPYS+LFAP+ERKDIVISTSCWTLMAVLLVYLSFVFGP Q+
Sbjct: 181 LAYPFYLMWRSPGKEGSHFNPYSDLFAPSERKDIVISTSCWTLMAVLLVYLSFVFGPFQI 240

Query: 241 FKIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH 300
           FK+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH
Sbjct: 241 FKLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHH 300

Query: 301 DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHY 360
           DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLV NLVNSL+QDHY
Sbjct: 301 DIGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPIHLVNNLVNSLKQDHY 360

Query: 361 VSDQGNIVFYQTDPYLYH 378
           VSDQGNIVFYQTDPYLYH
Sbjct: 361 VSDQGNIVFYQTDPYLYH 374

[Close]

BLAST of Spg005823.1 vs. ExPASy TrEMBL
Match: A0A6J1K635 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX=3661 GN=LOC111492652 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.4e-207
Identity = 339/376 (90.16%), Postives = 355/376 (94.41%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  S   N    A GTAAPP FDPSAPPPFRISEIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1   MAIPLPASRPGNDTVYANGTAAPPSFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RDF ++S LVAAA  LDSWLVWP+YW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61  RDFVIISALVAAAAYLDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHSAILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRF+LPFPI 
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS++FAPNERKDIVISTSCWTLMAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPTQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFV+WLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWIFVIWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLINNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNS RQDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD+GN+VFYQTDPYLY
Sbjct: 361 SDEGNVVFYQTDPYLY 376

[Close]

BLAST of Spg005823.1 vs. ExPASy TrEMBL
Match: A0A6J1GAA9 (omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata OX=3662 GN=LOC111452212 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 5.2e-207
Identity = 336/376 (89.36%), Postives = 355/376 (94.41%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL  S   N   +A GTAAP PFDPSAPPPFRISEIRAAIPPHCWVKNPWRSL+YVL
Sbjct: 1   MAIPLPASRPGNDTVHANGTAAPHPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RDF ++S LVAAA   DSWLVWP+YW+AQGTMFWAIFVLGHDCGHGSFSNS+ALNSFMGL
Sbjct: 61  RDFVIISALVAAAAYFDSWLVWPIYWIAQGTMFWAIFVLGHDCGHGSFSNSTALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHSAILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLERRTRILRF+LPFPI 
Sbjct: 121 ILHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLERRTRILRFSLPFPIF 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS++FAPNERKDIVISTSCWT+MAVLLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDIFAPNERKDIVISTSCWTIMAVLLVYLSFVFGPTQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYW+FV+WLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGL NNIHHD
Sbjct: 241 KLYGVPYWVFVIWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLINNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNS RQDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPLHLVKNLVNSFRQDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD+GN+VFYQTDPYLY
Sbjct: 361 SDEGNVVFYQTDPYLY 376

[Close]

BLAST of Spg005823.1 vs. ExPASy TrEMBL
Match: A0A0A0LVJ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.4e-206
Identity = 343/377 (90.98%), Postives = 355/377 (94.16%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           MAIPL        N NAT  AAP PFDPSAPPPFRISEIRAAIPPHCWVK+PWRSL+YVL
Sbjct: 1   MAIPLL------ANANAT-AAAPAPFDPSAPPPFRISEIRAAIPPHCWVKSPWRSLLYVL 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RD A+VS LVAAA+  DSWLVWP+YWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL
Sbjct: 61  RDLAIVSALVAAALFFDSWLVWPIYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           LLHS ILVPYHGWRISHRTHHQNHGNVEKDESWVP+TKKTYKQLE+RTRILRFTLPFPIL
Sbjct: 121 LLHSFILVPYHGWRISHRTHHQNHGNVEKDESWVPLTKKTYKQLEKRTRILRFTLPFPIL 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYPFYLMWRSPGKEGSHFNPYS+LFAP ER+DIVISTSCWTLMA LLVYLSFVFGP Q+F
Sbjct: 181 AYPFYLMWRSPGKEGSHFNPYSDLFAPGERRDIVISTSCWTLMAALLVYLSFVFGPFQIF 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPYWIFVMWLD VTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD
Sbjct: 241 KLYGVPYWIFVMWLDVVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIP HLVKNLVNSL+QDHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPTHLVKNLVNSLKQDHYV 360

Query: 361 SDQGNIVFYQTDPYLYH 378
           SDQGNIVFYQTDPYLYH
Sbjct: 361 SDQGNIVFYQTDPYLYH 370

[Close]

BLAST of Spg005823.1 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 580.1 bits (1494), Expect = 1.3e-165
Identity = 258/351 (73.50%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDP APPPF +++IRAAIP HCWVKNPW+SL YV+RD A+V  L A A  L++W+VWPLY
Sbjct: 88  FDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLY 147

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           WLAQGTMFWA+FVLGHDCGHGSFSN   LNS +G LLHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 148 WLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHG 207

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           +VE DESW PM++K Y  L++ TR  RFTLP  +LAYPFYL  RSPGK+GSH++P S+LF
Sbjct: 208 HVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLF 267

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P ERKD++ ST+CWT MA LLV L+F  GPIQM K+YG+PYWI VMWLDFVTYLHHHG+
Sbjct: 268 LPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGH 327

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
           E KLPWYRG+EW+YLRGGLTT+DRDYGL NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 328 EDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 387

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYLY 377
           K VLGKYYREP KSGP+P HL++ L  S+++DHYVSD+G +V+Y+ DP LY
Sbjct: 388 KPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGEVVYYKADPNLY 438

[Close]

BLAST of Spg005823.1 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 577.8 bits (1488), Expect = 6.4e-165
Identity = 255/350 (72.86%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDP APPPF +++IRAAIP HCWVKNPW S+ YV+RD A+V  L A A   ++WL+WPLY
Sbjct: 81  FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           W AQGTMFWA+FVLGHDCGHGSFSN   LNS  G LLHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           +VE DESW P+ +  YK LE+ T++ RFTLPFP+LAYPFYL  RSPGK+GSH++P S+LF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P E+KD++ ST+CWT MA LLV L+FV GPIQM K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEATKAA 325
           E KLPWYRG+EW+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHLVEAT+AA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAA 380

Query: 326 KGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYVSDQGNIVFYQTDPYL 376
           K VLGKYYREPK SGP+P HL+ +L+ S++QDH+VSD G++V+Y+ DP L
Sbjct: 381 KPVLGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVYYEADPKL 430

[Close]

BLAST of Spg005823.1 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 573.5 bits (1477), Expect = 1.2e-163
Identity = 262/376 (69.68%), Postives = 311/376 (82.71%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           M + +      NG+  A        FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1   MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RD   V+ L  AAV +DSW +WPLYW AQGT+FWAIFVLGHDCGHGSFS+   LNS +G 
Sbjct: 61  RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHS ILVPYHGWRISHRTHHQNHG+VE DESWVP+ ++ YK+L   TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYP YL +RSPGKEGSHFNPYS LFAP+ERK I  ST+CW++M V L+ LSFVFGP+ + 
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHD 300
           K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+EW+YLRGGLTT+DRDYG+FNNIHHD
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHD 300

Query: 301 IGTHVIHHLFPQIPHYHLVEATKAAKGVLGKYYREPKKSGPIPFHLVKNLVNSLRQDHYV 360
           IGTHVIHHLFPQIPHYHLV+ATKAAK VLG+YYREPK SG IP HLV++LV S+++DHYV
Sbjct: 301 IGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYV 360

Query: 361 SDQGNIVFYQTDPYLY 377
           SD G+IVFY+TDP LY
Sbjct: 361 SDTGDIVFYETDPDLY 376

[Close]

BLAST of Spg005823.1 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 498.8 bits (1283), Expect = 3.8e-141
Identity = 221/296 (74.66%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 26  FDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVLRDFAVVSVLVAAAVSLDSWLVWPLY 85
           FDP APPPF +++IRAAIP HCWVKNPW S+ YV+RD A+V  L A A   ++WL+WPLY
Sbjct: 81  FDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLY 140

Query: 86  WLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGLLLHSAILVPYHGWRISHRTHHQNHG 145
           W AQGTMFWA+FVLGHDCGHGSFSN   LNS  G LLHS+ILVPYHGWRISHRTHHQNHG
Sbjct: 141 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 200

Query: 146 NVEKDESWVPMTKKTYKQLERRTRILRFTLPFPILAYPFYLMWRSPGKEGSHFNPYSELF 205
           +VE DESW P+ +  YK LE+ T++ RFTLPFP+LAYPFYL  RSPGK+GSH++P S+LF
Sbjct: 201 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 260

Query: 206 APNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMFKIYGVPYWIFVMWLDFVTYLHHHGY 265
            P E+KD++ ST+CWT MA LLV L+FV GPIQM K+YG+PYWIFVMWLDFVTYLHHHG+
Sbjct: 261 LPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHGH 320

Query: 266 EQKLPWYRGEEWTYLRGGLTTVDRDYGLFNNIHHDIGTHVIHHLFPQIPHYHLVEA 322
           E KLPWYRG+EW+YLRGGLTT+DRDYG  NNIHHDIGTHVIHHLFPQIPHYHLVEA
Sbjct: 321 EDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEA 376

[Close]

BLAST of Spg005823.1 vs. TAIR 10
Match: AT2G29980.2 (fatty acid desaturase 3 )

HSP 1 Score: 392.9 bits (1008), Expect = 2.9e-109
Identity = 180/275 (65.45%), Postives = 217/275 (78.91%), Query Frame = 0


Query: 1   MAIPLSPSATCNGNGNATGTAAPPPFDPSAPPPFRISEIRAAIPPHCWVKNPWRSLVYVL 60
           M + +      NG+  A        FDPSA PPF+I +IRAAIP HCWVK+P RS+ YV+
Sbjct: 1   MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV 60

Query: 61  RDFAVVSVLVAAAVSLDSWLVWPLYWLAQGTMFWAIFVLGHDCGHGSFSNSSALNSFMGL 120
           RD   V+ L  AAV +DSW +WPLYW AQGT+FWAIFVLGHDCGHGSFS+   LNS +G 
Sbjct: 61  RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH 120

Query: 121 LLHSAILVPYHGWRISHRTHHQNHGNVEKDESWVPMTKKTYKQLERRTRILRFTLPFPIL 180
           +LHS ILVPYHGWRISHRTHHQNHG+VE DESWVP+ ++ YK+L   TR+LR+T+P P+L
Sbjct: 121 ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML 180

Query: 181 AYPFYLMWRSPGKEGSHFNPYSELFAPNERKDIVISTSCWTLMAVLLVYLSFVFGPIQMF 240
           AYP YL +RSPGKEGSHFNPYS LFAP+ERK I  ST+CW++M V L+ LSFVFGP+ + 
Sbjct: 181 AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL 240

Query: 241 KIYGVPYWIFVMWLDFVTYLHHHGYEQKLPWYRGE 276
           K+YGVPY IFVMWLD VTYLHHHG+++KLPWYRG+
Sbjct: 241 KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGK 275

The following BLAST results are available for this feature:

BLAST of Spg005823.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0031835.1	1.2e-210	92.84	acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum [Cucumis me...	[more]
XP_008457364.1	3.0e-209	92.33	PREDICTED: acyl-lipid omega-3 desaturase (cytochrome b5), endoplasmic reticulum ...	[more]
XP_022997807.1	2.8e-207	90.16	omega-3 fatty acid desaturase, endoplasmic reticulum-like [Cucurbita maxima]	[more]
XP_023523732.1	3.7e-207	89.63	omega-3 fatty acid desaturase, endoplasmic reticulum-like isoform X1 [Cucurbita ...	[more]
XP_023523733.1	4.9e-207	89.63	omega-3 fatty acid desaturase, endoplasmic reticulum-like isoform X2 [Cucurbita ...	[more]

BLAST of Spg005823.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48626	1.8e-167	76.35	Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum OX=409...	[more]
P48625	8.2e-165	73.30	Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max OX=3847 GN=F...	[more]
P32291	1.4e-164	73.35	Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Vigna radiata var. radia...	[more]
P46310	1.8e-164	73.50	sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis th...	[more]
P48622	9.0e-164	72.86	Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloropla...	[more]

BLAST of Spg005823.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SRR2	6.0e-211	92.84	Acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ...	[more]
A0A1S3C608	1.5e-209	92.33	acyl-lipid omega-3 desaturase (Cytochrome b5), endoplasmic reticulum OS=Cucumis ...	[more]
A0A6J1K635	1.4e-207	90.16	omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita maxima OX...	[more]
A0A6J1GAA9	5.2e-207	89.36	omega-3 fatty acid desaturase, endoplasmic reticulum-like OS=Cucurbita moschata ...	[more]
A0A0A0LVJ0	4.4e-206	90.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G532210 PE=3 SV=1	[more]

BLAST of Spg005823.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11170.1	1.3e-165	73.50	fatty acid desaturase 7	[more]
AT5G05580.1	6.4e-165	72.86	fatty acid desaturase 8	[more]
AT2G29980.1	1.2e-163	69.68	fatty acid desaturase 3	[more]
AT5G05580.2	3.8e-141	74.66	fatty acid desaturase 8	[more]
AT2G29980.2	2.9e-109	65.45	fatty acid desaturase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021863	Fatty acid desaturase, N-terminal	PFAM	PF11960	DUF3474	coord: 21..71 e-value: 7.7E-23 score: 81.2
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 78..333 e-value: 2.9E-34 score: 119.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR32100	OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC	coord: 19..375
None	No IPR available	PANTHER	PTHR32100:SF52	TEMPERATURE-SENSITIVE SN-2 ACYL-LIPID OMEGA-3 DESATURASE (FERREDOXIN), CHLOROPLASTIC	coord: 19..375
None	No IPR available	CDD	cd03507	Delta12-FADS-like	coord: 50..318 e-value: 9.75272E-74 score: 226.723

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg005823	Spg005823	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg005823.1-five_prime_utr	Spg005823.1-five_prime_utr-scaffold11:930946..931266	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg005823.1-cds	Spg005823.1-cds-scaffold11:931267..931574	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:931645..931734	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:931826..931892	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:931974..932066	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:932144..932329	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:932643..932723	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:932829..932966	CDS
Spg005823.1-cds	Spg005823.1-cds-scaffold11:934714..934884	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg005823.1-three_prime_utr	Spg005823.1-three_prime_utr-scaffold11:934885..934972	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg005823.1	Spg005823.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0006636	unsaturated fatty acid biosynthetic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function	GO:0042389	omega-3 fatty acid desaturase activity