Spg003034.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg003034.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 76C1-like
Location	scaffold12: 38491984 .. 38492355 (-)
Sequence length	372
RNA-Seq Expression	Spg003034.1
Synteny	Spg003034.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAAGCTCCGCCTTGGAACCAAGCTCTGCATCGTTCTCAGCTCCCCTTCCTCCGTCAATGAAGCCCTCCGCCACCACGAAACCGTCTTCGCCAACCGAGATTCTACTGTCGCTGCTCGTCTCGTCAGCTACAGCGGCGCCGACATTGTCTTCACCCAGGACGTTGACGATTGGAAGAAGCTGAGGAAAATCTTCGCCCGCAAAATGCTTAGCAAATCAAATCTCGATGCGACCTACTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTCGAATCGGCCGGAGCCCCAATCGACGTCGGCAAAGTGGGTTTCTCCGCTGCTCTGAAATCGGTTATGGCGATGACATGGGGCGGCTGA

mRNA sequence

ATGCTCAAGCTCCGCCTTGGAACCAAGCTCTGCATCGTTCTCAGCTCCCCTTCCTCCGTCAATGAAGCCCTCCGCCACCACGAAACCGTCTTCGCCAACCGAGATTCTACTGTCGCTGCTCGTCTCGTCAGCTACAGCGGCGCCGACATTGTCTTCACCCAGGACGTTGACGATTGGAAGAAGCTGAGGAAAATCTTCGCCCGCAAAATGCTTAGCAAATCAAATCTCGATGCGACCTACTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTCGAATCGGCCGGAGCCCCAATCGACGTCGGCAAAGTGGGTTTCTCCGCTGCTCTGAAATCGGTTATGGCGATGACATGGGGCGGCTGA

Coding sequence (CDS)

ATGCTCAAGCTCCGCCTTGGAACCAAGCTCTGCATCGTTCTCAGCTCCCCTTCCTCCGTCAATGAAGCCCTCCGCCACCACGAAACCGTCTTCGCCAACCGAGATTCTACTGTCGCTGCTCGTCTCGTCAGCTACAGCGGCGCCGACATTGTCTTCACCCAGGACGTTGACGATTGGAAGAAGCTGAGGAAAATCTTCGCCCGCAAAATGCTTAGCAAATCAAATCTCGATGCGACCTACTCTGTGCGAAGACAAGAGGTGAGAAAAGTGATTAAAGGCGTGTTCGAATCGGCCGGAGCCCCAATCGACGTCGGCAAAGTGGGTTTCTCCGCTGCTCTGAAATCGGTTATGGCGATGACATGGGGCGGCTGA

Protein sequence

MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWKKLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMTWGG

Homology

[Close]

BLAST of Spg003034.1 vs. NCBI nr
Match: KAA0039671.1 (7-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 199.1 bits (505), Expect = 2.2e-47
Identity = 100/123 (81.30%), Postives = 112/123 (91.06%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           +LKL LGTKLCIVL+SPSSVNEALRH ET+F+ RD+TV A L SY G+DIVFTQD ++WK
Sbjct: 77  ILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDENNWK 136

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKSNLDA+YS+RRQEVRKVIKGVFESAG PID+GK+GF AALKSVMAMT
Sbjct: 137 KLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSVMAMT 196

Query: 121 WGG 124
           WGG
Sbjct: 197 WGG 199

[Close]

BLAST of Spg003034.1 vs. NCBI nr
Match: XP_008437141.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 199.1 bits (505), Expect = 2.2e-47
Identity = 100/123 (81.30%), Postives = 112/123 (91.06%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           +LKL LGTKLCIVL+SPSSVNEALRH ET+F+ RD+TV A L SY G+DIVFTQD ++WK
Sbjct: 77  ILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDENNWK 136

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKSNLDA+YS+RRQEVRKVIKGVFESAG PID+GK+GF AALKSVMAMT
Sbjct: 137 KLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSVMAMT 196

Query: 121 WGG 124
           WGG
Sbjct: 197 WGG 199

[Close]

BLAST of Spg003034.1 vs. NCBI nr
Match: XP_023550541.1 (ferruginol synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 195.3 bits (495), Expect = 3.1e-46
Identity = 96/122 (78.69%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLR+G KLC+VL+SPSS+NEA RHHETVF NRD+TV A L SY G+ IVFTQD DDWK
Sbjct: 81  VFKLRIGNKLCVVLNSPSSINEAFRHHETVFPNRDTTVCALLCSYDGSGIVFTQDGDDWK 140

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKS LDA+YSVRRQEVRKVIKGVFESAG PID+GKVGF A +KSV+AMT
Sbjct: 141 KLRKIFVRKMLSKSFLDASYSVRRQEVRKVIKGVFESAGTPIDIGKVGFLATMKSVLAMT 200

Query: 121 WG 123
           WG
Sbjct: 201 WG 202

[Close]

BLAST of Spg003034.1 vs. NCBI nr
Match: XP_038906861.1 (labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 191.8 bits (486), Expect = 3.5e-45
Identity = 94/123 (76.42%), Postives = 109/123 (88.62%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLRLGTKLC+VLSSPSSVNEAL H ETV  NRDST++A L +Y GADI+F+QD  DWK
Sbjct: 83  IFKLRLGTKLCVVLSSPSSVNEALHHQETVLDNRDSTISALLATYGGADILFSQDEGDWK 142

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKSNLDA+YS+RR+EVRKVIKG FESAG+PI++GK+GF A +KSVMAMT
Sbjct: 143 KLRKIFTRKMLSKSNLDASYSLRRKEVRKVIKGAFESAGSPINIGKLGFFATVKSVMAMT 202

Query: 121 WGG 124
           WGG
Sbjct: 203 WGG 205

[Close]

BLAST of Spg003034.1 vs. NCBI nr
Match: XP_008437142.1 (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 186.8 bits (473), Expect = 1.1e-43
Identity = 91/123 (73.98%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLRLG KLC VL+SPSS+NE LRHHETVF NRD+TV+ARL +Y G+DIVF+Q+  DWK
Sbjct: 78  IFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSARLCTYGGSDIVFSQNEGDWK 137

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIFARKMLSK+NLDA+Y +RRQEVRK+IKGV ESAG PID G++ F AA+KSVMAM 
Sbjct: 138 KLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSVMAMI 197

Query: 121 WGG 124
           WGG
Sbjct: 198 WGG 200

[Close]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot
Match: F4JW83 (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-11
Identity = 36/110 (32.73%), Postives = 65/110 (59.09%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           +L LRLG     V+SSP    + L+  + VF+NR +T+A R ++Y G+D+ F      W+
Sbjct: 66  LLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNYGPFWR 125

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFS 111
           ++RK++   + S+   ++  SV  +EV K ++ V  + G P+++ K+ FS
Sbjct: 126 RMRKLYVMMLFSRKRAESWVSV-DEEVHKSVRLVASNVGKPLNICKLAFS 174

[Close]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot
Match: O48923 (Cytochrome P450 71D10 OS=Glycine max OX=3847 GN=CYP71D10 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 3.4e-11
Identity = 39/122 (31.97%), Postives = 75/122 (61.48%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           ++ L+LG    I+++SP    E ++ H+  F++R   V +R+VSY+G+ IVF+Q  D W+
Sbjct: 79  LMHLKLGEVSNIIVTSPEMAQEIMKTHDLNFSDRPDFVLSRIVSYNGSGIVFSQHGDYWR 138

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           +LRKI   ++L+   + +  S+R +EV +++K +  +A    + G   F+   +S+ +MT
Sbjct: 139 QLRKICTVELLTAKRVQSFRSIREEEVAELVKKIAATAS---EEGGSIFNLT-QSIYSMT 196

Query: 121 WG 123
           +G
Sbjct: 199 FG 196

[Close]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 5.8e-11
Identity = 34/103 (33.01%), Postives = 60/103 (58.25%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           ++ L+LG    +V++SP    E L+ H+  FA+R   +A  +V Y+ +DI F    D W+
Sbjct: 67  LMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPEIVCYNRSDIAFCPYGDYWR 126

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPID 104
           ++RKI   ++LS  N+ +  S+RR EV +++  V  S   P++
Sbjct: 127 QMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEPVN 169

[Close]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot
Match: Q6WKZ0 (Cytochrome P450 71D94 OS=Mentha gracilis OX=241069 GN=CYP71D94 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.3e-10
Identity = 33/101 (32.67%), Postives = 64/101 (63.37%), Query Frame = 0


Query: 4   LRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWKKLR 63
           ++LG    +VLSS  +  +A++  +  FA+R  +V +R++ Y   DI+F+   D W ++R
Sbjct: 70  VQLGEVYSVVLSSAEAAKQAMKVLDPNFADRFDSVGSRIMWYDNDDIIFSPYNDHWHQMR 129

Query: 64  KIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDV 105
           KI   ++LS  N+ +   +R++E+ ++I+ + +S GAP+DV
Sbjct: 130 KICVSELLSPKNVRSFGFIRQEEIERLIRVMKQSEGAPVDV 170

[Close]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot
Match: O81971 (Cytochrome P450 71D9 OS=Glycine max OX=3847 GN=CYP71D9 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-10
Identity = 34/106 (32.08%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           ++ L+LG    IV+SSP    E ++ H+ +FA+R   +AA ++ Y    + FT   D W+
Sbjct: 71  LMHLKLGEVSTIVVSSPEYAKEVMKTHDHIFASRPYVLAAEIMDYDFKGVAFTPYGDYWR 130

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGK 107
           +LRKIFA ++LS   + +   +R + +   IK +    G+ ++V K
Sbjct: 131 QLRKIFALELLSSKRVQSFQPIREEVLTSFIKRMATIEGSQVNVTK 176

[Close]

BLAST of Spg003034.1 vs. ExPASy TrEMBL
Match: A0A5A7TDF0 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00010 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-47
Identity = 100/123 (81.30%), Postives = 112/123 (91.06%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           +LKL LGTKLCIVL+SPSSVNEALRH ET+F+ RD+TV A L SY G+DIVFTQD ++WK
Sbjct: 77  ILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDENNWK 136

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKSNLDA+YS+RRQEVRKVIKGVFESAG PID+GK+GF AALKSVMAMT
Sbjct: 137 KLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSVMAMT 196

Query: 121 WGG 124
           WGG
Sbjct: 197 WGG 199

[Close]

BLAST of Spg003034.1 vs. ExPASy TrEMBL
Match: A0A1S3ATA1 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-47
Identity = 100/123 (81.30%), Postives = 112/123 (91.06%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           +LKL LGTKLCIVL+SPSSVNEALRH ET+F+ RD+TV A L SY G+DIVFTQD ++WK
Sbjct: 77  ILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYGGSDIVFTQDENNWK 136

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIF RKMLSKSNLDA+YS+RRQEVRKVIKGVFESAG PID+GK+GF AALKSVMAMT
Sbjct: 137 KLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGKLGFVAALKSVMAMT 196

Query: 121 WGG 124
           WGG
Sbjct: 197 WGG 199

[Close]

BLAST of Spg003034.1 vs. ExPASy TrEMBL
Match: A0A5A7T9J1 (Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00030 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.4e-44
Identity = 91/123 (73.98%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLRLG KLC VL+SPSS+NE LRHHETVF NRD+TV+ARL +Y G+DIVF+Q+  DWK
Sbjct: 78  IFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSARLCTYGGSDIVFSQNEGDWK 137

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIFARKMLSK+NLDA+Y +RRQEVRK+IKGV ESAG PID G++ F AA+KSVMAM 
Sbjct: 138 KLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSVMAMI 197

Query: 121 WGG 124
           WGG
Sbjct: 198 WGG 200

[Close]

BLAST of Spg003034.1 vs. ExPASy TrEMBL
Match: A0A1S3ATW0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.4e-44
Identity = 91/123 (73.98%), Postives = 107/123 (86.99%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLRLG KLC VL+SPSS+NE LRHHETVF NRD+TV+ARL +Y G+DIVF+Q+  DWK
Sbjct: 78  IFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSARLCTYGGSDIVFSQNEGDWK 137

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIFARKMLSK+NLDA+Y +RRQEVRK+IKGV ESAG PID G++ F AA+KSVMAM 
Sbjct: 138 KLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGTPIDFGELSFLAAVKSVMAMI 197

Query: 121 WGG 124
           WGG
Sbjct: 198 WGG 200

[Close]

BLAST of Spg003034.1 vs. ExPASy TrEMBL
Match: A0A6J1DW75 (7-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC111023676 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.6e-42
Identity = 92/123 (74.80%), Postives = 104/123 (84.55%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KLRLGTKLC+VL+SPS+VNEAL   +TVFANRD TV A L SY G+DIV T D  DWK
Sbjct: 109 VFKLRLGTKLCVVLTSPSAVNEALHRQDTVFANRDPTVCALLASYGGSDIVITSDGADWK 168

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESAGAPIDVGKVGFSAALKSVMAMT 120
           KLRKIFARKMLSKSNL+A+Y VRR+EVR+ IKGVFESAG PIDVG+VGF A LKSVMAM 
Sbjct: 169 KLRKIFARKMLSKSNLEASYCVRRREVREAIKGVFESAGTPIDVGEVGFLATLKSVMAMI 228

Query: 121 WGG 124
           +GG
Sbjct: 229 FGG 231

[Close]

BLAST of Spg003034.1 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 92.0 bits (227), Expect = 3.5e-19
Identity = 50/125 (40.00%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KL LG+KL IV++SPS   E L+  +  F+NRD  +  R  +Y G DIV+T    +W+
Sbjct: 76  IFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWR 135

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESA--GAPIDVGKVGFSAALKSVMA 120
           +LRKI   K+LS+  LD+ Y +RR+EVR+  + ++E     +P+ VG   F   +   M 
Sbjct: 136 QLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMN 195

Query: 121 MTWGG 124
           M WGG
Sbjct: 196 MLWGG 200

[Close]

BLAST of Spg003034.1 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 90.5 bits (223), Expect = 1.0e-18
Identity = 46/124 (37.10%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           ++K+ LG+KL IV+SSP    E L+ H+  FAN D     ++ +Y G DI+++     W+
Sbjct: 88  LMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGTHWR 147

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESA--GAPIDVGKVGFSAALKSVMA 120
           +LRK+   KM +  +L+A+YS RR+E R+ I  + E A  G+P+++G+  F +    V  
Sbjct: 148 RLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREGSPVNLGEQIFLSIFNVVTR 207

Query: 121 MTWG 123
           M WG
Sbjct: 208 MMWG 211

[Close]

BLAST of Spg003034.1 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 90.1 bits (222), Expect = 1.3e-18
Identity = 46/124 (37.10%), Postives = 74/124 (59.68%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           ++K+ LG+KL IV+SSP    E L+ H+  FAN D     ++ +Y G DI+++     W+
Sbjct: 119 LMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGTHWR 178

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESA--GAPIDVGKVGFSAALKSVMA 120
           +LRK+   KM +   L+A+YS RR+E R+ I  + E A  G+P+++G+  F +    V  
Sbjct: 179 RLRKLCVMKMFTTPTLEASYSTRREETRQTIVHMSEMAREGSPVNLGEQIFLSIFNVVTR 238

Query: 121 MTWG 123
           M WG
Sbjct: 239 MMWG 242

[Close]

BLAST of Spg003034.1 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 89.7 bits (221), Expect = 1.7e-18
Identity = 45/125 (36.00%), Postives = 78/125 (62.40%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KL LG+KL +V++SPS  +E L+  +  F+N D  + AR V+Y G D+V+     +W+
Sbjct: 78  IFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWR 137

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESA--GAPIDVGKVGFSAALKSVMA 120
            LRK+ A K+ S+  LD+ Y +RR+E+R+  + +++     +P++VG+  F   +  +M 
Sbjct: 138 MLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMN 197

Query: 121 MTWGG 124
           M WGG
Sbjct: 198 MLWGG 202

[Close]

BLAST of Spg003034.1 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 84.3 bits (207), Expect = 7.3e-17
Identity = 43/125 (34.40%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 1   MLKLRLGTKLCIVLSSPSSVNEALRHHETVFANRDSTVAARLVSYSGADIVFTQDVDDWK 60
           + KL LG+KL +V+++PS   E L+  +  F+N D  + AR V+Y G D+V+     +W+
Sbjct: 76  IFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWR 135

Query: 61  KLRKIFARKMLSKSNLDATYSVRRQEVRKVIKGVFESA--GAPIDVGKVGFSAALKSVMA 120
            LRK+   K+LS   L++ Y +RR+E+R+  + +++     +P++VG+  F   +   M 
Sbjct: 136 MLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNLTMN 195

Query: 121 MTWGG 124
           M WGG
Sbjct: 196 MLWGG 200

The following BLAST results are available for this feature:

BLAST of Spg003034.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039671.1	2.2e-47	81.30	7-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa]	[more]
XP_008437141.1	2.2e-47	81.30	PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo]	[more]
XP_023550541.1	3.1e-46	78.69	ferruginol synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038906861.1	3.5e-45	76.42	labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida]	[more]
XP_008437142.1	1.1e-43	73.98	PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo]	[more]

BLAST of Spg003034.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JW83	1.5e-11	32.73	Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1	[more]
O48923	3.4e-11	31.97	Cytochrome P450 71D10 OS=Glycine max OX=3847 GN=CYP71D10 PE=2 SV=1	[more]
A6YIH8	5.8e-11	33.01	Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1	[more]
Q6WKZ0	1.3e-10	32.67	Cytochrome P450 71D94 OS=Mentha gracilis OX=241069 GN=CYP71D94 PE=2 SV=1	[more]
O81971	2.2e-10	32.08	Cytochrome P450 71D9 OS=Glycine max OX=3847 GN=CYP71D9 PE=2 SV=1	[more]

BLAST of Spg003034.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TDF0	1.1e-47	81.30	7-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3ATA1	1.1e-47	81.30	7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 ...	[more]
A0A5A7T9J1	5.4e-44	73.98	Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S3ATW0	5.4e-44	73.98	flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=...	[more]
A0A6J1DW75	6.6e-42	74.80	7-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC11102367...	[more]

BLAST of Spg003034.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12300.1	3.5e-19	40.00	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]
AT4G22710.1	1.0e-18	37.10	cytochrome P450, family 706, subfamily A, polypeptide 2	[more]
AT4G22690.1	1.3e-18	37.10	cytochrome P450, family 706, subfamily A, polypeptide 1	[more]
AT4G12310.1	1.7e-18	36.00	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]
AT4G12320.1	7.3e-17	34.40	cytochrome P450, family 706, subfamily A, polypeptide 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..119 e-value: 4.4E-11 score: 42.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..123 e-value: 7.5E-18 score: 66.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..122
None	No IPR available	PANTHER	PTHR24299:SF20	SUBFAMILY NOT NAMED	coord: 2..122
None	No IPR available	PANTHER	PTHR24299	CYTOCHROME P450 FAMILY 1	coord: 2..122

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg003034	Spg003034	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg003034.1-cds	Spg003034.1-cds-scaffold12:38491984..38492355	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg003034.1	Spg003034.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006865	amino acid transport
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0015293	symporter activity