Spg002974.1 (mRNA) Sponge gourd (cylindrica) v1

Overview
NameSpg002974.1
TypemRNA
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmitogen-activated protein kinase kinase kinase YODA-like
Locationscaffold6: 1424354 .. 1432099 (+)
Sequence length2850
RNA-Seq ExpressionSpg002974.1
SyntenySpg002974.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCGCTAAGGCATACTCTCTCTTTCTCTTTCATTTCTCTCTCTCCCTTTCGTTACGCGATTTTCTCAATCCCACAATTCATCATAAAATGGAGAAGATTGATGTAACGCTTCGCCTCTGATAATCTGTGATTTCAGATGATTAGGGTTTCGATTTTTTTCTAATTTCAGTGTTCAGTTTGAATTATTGCGGGAGTCTACCTCACTGGGATTTGGGCGAGTATAGTGGGATTCTACTGGAATTTGCAGTTTCCAACATTTAAGGTAGCACTTCTTCGTTTCATTCTTCTAATCTAATTTTAGTAAATTACTGATTATCTGGTTTTCGTTTCGTTAGTCACTGTGCTAGTTTATCTGTATTAGATTCTGGGTAGATCTGTATCGTAGAGTCGAGTCTTGAGGATTATGGAAGTGTAGCTCGTTGTTTGATTTTCTAAACGGCCGTGGGAAAGAAAAGGAGGAAATTTCGAAGTGTCGTTGGCGAAGACGGAGCTTAATGGTTCAGTTCGTTTTGTTGCAGTGGGAATTTCGGTTGCATCAGATGACATAAGCGATTAGAGGTTGGATTTGAATTTATTTCTGTGGTCTAATTTATCTTTTTTCCCCTTTTCTTGATAAGAGTAGAAGAGAGCAAGAATCATTTGTTTATATGTTGGATATGTTGATTATCTCATCGTTTTTACTTGTTCAGCGCTGTAATTAATGATCTCCAACTCTCATTAACCATTGGTTATCTTTGGTGGGGGGGGGGGGGGTTGTTGCTAGTCAAAACAAATCAAAAAGTAATTAAGGGAGAAATGGGACAAAATATTATAGCCCCAATTAAGTAGATTTGGGGATTATGAAAAGTCTCCCCAATTTATCATGATAAGATAATAGAAAATTAACTGTCTCCAATTAAGTAGACTTGGGGATTATAAAAAGAGTCCAATAGTGCTTGAAAAATCTCTCCACTATCCTCTTAATTCAAAGGTTCAAAGGTATTTTGTGATTGTGGACCATTTCAGCCATGAAATGCACTAATTGTACAATAGTCCTGGCCTCCACCATAAAAGATTCCAAAAGTTTAAGAGAAATCTACCCAGTTTTTGCAGTATTTCTAGTGAAGAATATAAAGTTAATGGATTCAGAGTCTGCTTGAAGCATGTTTGTTACATCTCAAGTCATATGAAATCCTGAATTTCTTGCATGCATAAGCTTACTTTAAGAGTGTTCATGCTATCAAGGACTGGTTTTTTTATTTTTTATTTTTTAAAAAATTTTTACAATAATAAATAGAATGCTGTTTCTCCAACAGATACATCATCTTCGTAATATCTTATGCAGGATTTTGTTACAAGTTCTTGATTATTGGGATTTCTCAAATTCTATCTGAAGTGGTGGTTTGGATACTCAGCCACAGCTTTACCAGCATGCCTATGGGGTAAAAAGTTACTGATGATATGAAGGGTATCATTTACTTGTGGGATGCTTTGGAAGTGTAACATCTGCAATCATCTATTTTCACGAAATATTTTCGCTCTGCAGAGTGAGTTCAATTTAGAGTCAGCTTCTTTGCTGTTTCCACATAATATAGAATAAAACCAAAAATGCCTTCATGGTGGGGGAAGCCATCATCCAAAGAAGTAAAGAAGAAAACGAGCAAGGAAAGTTTCATTGACTCATTACACCGTAAGTTTAAGAATTCACCTGAAGGTAAAGTAAGCAGTAGATCAGGAAGTTCTCGTAGACGTGGTGGTGACACTATATCTGAGAAGGGGTCTAAATCACCTCTATCAAGATCACCATCACCTTCCAAAGAAGTATCTAGATGTCAAAGTTTTGCTGAAAGATCACGTTCTCACCAACTTCCACTTCCAGAACTGCACCCTGTAGGTGTAGGTCGTACAGATTCTGGGATTAGTGTGACAGCAAAATCAAAATTAGAGAGAAGCTCCAAGACACCTTCATTCTTACCCCTCCCTAGACCTGCATGTATTCGAAGTAGGCCAGATCCTGCAGATTTGGATGGAGACTTGGTCACTGGTTCAGTCTCTGGTGAGTCCTCAAGTGATAGTGACGATCCCAATGATTCACGTCAACGTAGTCCTCAGGCAACTGACTATGACACTGGGACTAGAACTGTGATAGGTTCTACTGAACCTAGGTAAGCTCGCATAAGTCTTTATTTCATCTTTATCTTCCATTCTGGAGTCATTCTTTTCATTGTCCGTACTCTTAGTTGAATGGTTAAATAAGGTTACTTGCATCATTTTATTTGTTTGTCCTTGTTAGCAGTCTGCTTGCATCTGATGTATTGTTTTTTAAATTGTTGTTTTACTTTTCCCTGACATGTTTGCTGGTTTTTTTTCTGAAGTGCAACTCTCAAAGATCAGTCTTCTACTGTCGTGCAAATAAATTTAAAAGAGGGCAAGAAATTGGAAAGTCTTTCATTTCCTCATAAGAATGCTTCATTACCTAAACGGAGGCCTTTGAGCAGCAATGTAACAAATCTACAAGTTCCTCGCCATGGGGCTTTCTTTAGCGCTCCAGATAGTTCCATGTCTAGTCCCTCTAGAAGTCCTATGAGGGTATTTAGCACTGAGCAAGTCATGAACACTGCTGTTTGGGCTGGAAAGTCTCACCCAGATGTCATTTTAGGTGGATCTGGCCACTGCTCCAGTCCTGGTTCTGGTCACAATTCAGGGCACAACTCTATGGGAGGTGATATGGCGGGCCACTTCTTTTGGCAACAAAGTAGGGGTAGCCCCGAATATTCTCCAGTACCTAGTTCTCGAATGACTAGCCCCGGCCCAAGCTCCAGAATTCAAAGTGGTGCTGTCACACCTATTCATCCTAGAGCAGGAGCTCCACCTGCTGAGTCACAAACATGCTGGCCTGATGAGAAACAAACTCATCGTTTGCCTCTTCCTCCCATAGCAATCTCAAACTCTTCTCCATTTTCTCATTCAAATTCAGCAGTGACATCTCCTTCAGTGCCTAGGAGTCCAGGAAGGACAGAGACTCCAGCAAGCCCAGGTTCACGCTGGAAAAAGGGGAAGCTATTGGGTCGGGGCACATTTGGGCACGTATATGTTGGTTTTAATAGGTATGTAAACAAAAAATATTTAATTTTTCATTTTCATTGATTCAGGCAAAAGTTTGTTCATTCACATAACATTTATGATGCTGTAGGCTTATAGTCTTATCCGATATAAGTGATTATGATACCTTCATTATGGTATTGATTCCGTTTATCTCGTGAGGTCTGTCATTTTTAGTTCCTAACATGGTTGGTTTCTGATTTTTTTTTTGTTTATTATTTCAATGATTCAGTGAGAGTGGAGAAATGTGTGCAATGAAGGAGGTCACGTTATTTTCTGACGATGCAAAGTCTAAGGAAAGTGCTAAACAATTAATGCAGGTACAAGTTCTATCTTATTGCAGATCTCATCCAGTGCTTCCTTTTTTCACTCTACATTATTTCTTTGTTTTATTAAGAATTTACTTTTCAATTTAATGGCAGGAAATTGCTTTATTGAGTCGTTTACGGCATCCCAACATCGTGCAGTATTATGGTTCGGAAACAGTGAGTATTTTGCTTCAATTTTTTTTTGGCCGTTAACATTCTCATTATAATATTATGAAGAGAGGTGGTGAAGTGATCATCCCTTCCCCCTCGCATATGGAGGCCTCCCTAAAGTTTTGGGGAGGGGGGGGGGGGGGGTGGGGGTGGAGATACATATTGAAGAGAGATGGTGAGAGGTTTAAGATGATGATTTGTCAATGCAAGCAAATCAGATTTAATACCTAGTGTTGCAATGTCATTACTCTATTAAGGGGCTCGCTAAGCTTTGACAAAGGGACGTGTTAAATTGAAAAGGCTCGAGTAAATTTATTTTCTATTTTGGTAGAGTACCTTGGTCACTTTTTTTAACACAGGAACCCATTGTCCATGACCAAAAAAAGGCCAACTTTTCTATTACCTTCGTTATAACTACTGATATGTTAATTTGAGTAGGTAGTTGACAGTTTTGTATGAAGATAAGTCCTTGGAGGTGAAGTATCTATATTATGTAGGATTCAACTTGAGACCTAATTTCTAATAAGTTCCAATTAACTACTGTTGCTGCATACAATGGGTGTAGTTGGAAATCTGGGTTTTAGGCGGTTATGTAATCTTCCATGGTTTGGACATCTAATTTTTTATTTGAAATCATTCGTAACAGGTGGGTGATAAACTCTATATATATCTGGAATATGTATCTGGTGGCTCCATTTATAAATTACTACAAGAGTATGGACAATTTGGGGAACTAGCAATTCGTAGCTATACTCAACAAATACTATCAGGACTTGCATATTTGCATGCTAAAACTACAGTGCACAGGTGATTTAAACATCTTCGCCCACAGTTTGTCTGACTCATGCAATAGTGTAGTAGCTGCTACAATCTTATTGTTCCTTGTTTGCGGGACTTTAGACAGAATTGAACTTCTAACCTCTTCAGGGATATCAAAGGAGCAAACATACTTGTTGACCCAAATGGTCGTGTTAAATTGGCAGACTTTGGGATGGCGAAGCATGTAAGATAGCTCACAGTTTCCACCTTTACTATACCTCAAGTGATATGCTTTGAAAGCAATTCTAAATTTGACATCTCCAGCCATTAAATTTATAGAGCTAGTTGTTTTCTTTCTTTTTGTTTATCGATTATCAACAATCTTGTGAATATGAGGAGAAATGAACTGCGAAAAAAAACAATCTTGTGAATATGAAATAACCTGACAGGTGATATTTTAAATTATTATTAAAATTTAATTATTGAAGTCAATTGTAAGATGGCAAGTAAAAAGTTATTTCTAGTAGTTTTTATTAAATTGTGTTTTTTTTTCCTTTATTCTTTTTTTTTTTTTGGATAATAAACCAGACTTTCAATTAGAATGTGCACGGGGAGCGTATGTTATTGAAAACCCATGATTCTATTTCAATTTCAAATACAAATTTTAACATTTCATATTTATCTGCTATATTTACATTACATGTATTAGACATTACTTCAAAAGTTTACTGGTTTTGCGGTTATGAACTATTTTTGTATGTGGATATACTCAAATCCTTAAACTAAAAAAAAAAATTTACGTCTAATGGACATGTTAAGACAGTAGTAAATAAGTGATCATTATTCACTTAAATGATGTTGGTTAACGTGCTGCTGTTTTGTTTTTGTTTTTTCTTTTGGGTTTCAGATCACTGGCCAATCATGTCCCCTGTCGTTTAAGGGAAGCCCTTACTGGATGGCACCTGAGGTCAGAATTTATTGCATCTGTAAGCGAAAGCGATAAATATATGATGATACATATGCCTGACCCGTCACTTTCCCAGGTCATTAAGAACTCAAATGGTTGCAACCTTGCCGTTGATATTTGGAGTCTTGGATGTACTGTTCTGGAGATGGCTACTACAAAGCCACCTTGGAGCCAGTATGAAGGGGTGAGTATGGACGTTTTTTGAATATCTATCTAAGCTATAAAAGTGTCTTCAACGGTAGGAAATACACTTTCTATTGGACTGACGAGATAGCACATTTAAATTGCAAAAAATAATGTCGAAGGCTTTTGGTGTGCTATATTTTATTTTACTCTTAATTTTTTTTTAATTATTAAAGTAACCAAAAAGTTTCTATTAGGATTGTAAATTGTACGCATGAGTTAAGACACATCATTGTTTATTCTTCTCAAAGTTCATGGTTTCGCTGGTGGTCTATCTTGAATATTTCCTTGAAGCTTACTCTCACATTTGTTACATAGTGCCTTGTTCTTTGTTCTGACGGTGGGACATTTTATATTCTTGCTTAAAAGGTTGCTGCGATGTTTAAGATTGGCAATAGCAAAGAACTCCCAGTAATTCCAGAGCACCTCTCAGACGATGGGAAGGATTTTGTGAGACTATGTCTGCAACGAAACCCACATCATCGACCTACAGCTGCTCAACTTTTGGATCATCCTTTTGTCAAATATGCTGCACCTGTAGAGAGGCCTTTTTTGAGCTCTGAACCTTCTGATGCAACTCCTGGGGTTACAAACGGAGTGAAGATTTTGGTACACATACTTCTTTACATAGATTGTTTATTCATTGCTGTCTGTACTGGTTCAATTTTACTGCAGATGTGTTATAACAGCTTCTCCTTAATTTGCATGGTGCAAAAGATCTATGCTTTGCTGTCTTACCGTTTCTCTTACTGGATTCTTATTTTTTATATATACTGATATTTTAGTCGAATACTAAGCTAAGCTAGGCGACCAGCTGTGAGTCTATAACAAATTAGTAGAGTTTTGATATCTAGATAGAACTTTGGGTCATTTGGATTAGAATCTGGAAAGATTCTAGTGCCTCAGCCGGGAAAATCTTTTTCTATTTCAGTAGTTCAGTAAATGATTTTTTTTTTCCCTTTTCTGTCCTTGGTTCTGAGTGTTGTCTGTTAATCACTTCCAGGGTATTGGTCAATCACGGACTACTTCTTTGGACTCAGATAGCAGACTTGCAATTCATTCTTCAAGAGTCTCAAAAGCTGTTCTTCATGCCAGGTTCACTCTTCCCCCTCAACCCCAACCCCCAACAAATAAATAAATAAATTATGTTTTACTGACTGAGACAAGATTTAATTCTGATTTCATTTTCCATCCAGCGAAATCAGTATTCCCAGAAACATATCATGTCCTGTTTCACCAATTGGAAGTCCTCTTTTGCACTCGCGATCACCTCAACATCCCAGTGGAAGAATGTCGCCTTCACCTATATCAAGTCCACGAACCACTTCGGGCTCGTCAACGCCTTTGACAGGATGTGGTGGTGCAATTCCTTACAACCACCTGAAACAAGCTATATACTTGCAAGAGGGATTTGGAAGCATGCCAAAGTCCTTAAATAACAGTCCGTACAGCAGTGGTCTCTCCTTCCATGATTCAAATCCCGATATTTTTCGAGGGTTGCAGCCTGGGACTCCCATCTTCTCTGAAATGATACCAGAAAATGATATCTTGGGAAAGCAGGTTGGAAGGCCTGCTTATGGTGAATTGTATGATGGACAGCAGGTCTTGGCTGATCGAGTGTCTCGACAGCTCCTGAGGGATCATGTTAAAACAAACCCTTCTCTTGATTTGAGTCCTAGCCCTACATTGTCTGGCCGCATGAATGGTATCTGACAACGAATGCGTCGACCTACTAGAGCTAGTTGTCAGGAGTAAGAAGCTTTCTTCAGATCGGAAAGATTAATACCTAATCATTATTTGCCAAAACACGTTTATATAAGAACAGAGGGAGAAGGAATAGTAGACTAAGATTGATTTTCTTAAGGTGGAAGTAGCTGGATATGAAGTCTTCCACCACCGAACCAGTGGAGATGATTCTTTTGACATTTTGATTCTTGAAGGATAAGAAGGCTCACCTGTGTGACTAAATTAACGTCTGGTTCGATTTGATCCGGCGATGACCAAGGTATCAGCTTGTACAGTGTTCGAGTGTTCGATTTATCATCTGACCAACTAATCTGAGATGCAAAATTTTAATTTTGTTCATGCGGGATGACTTCTGATGATCCAATGTGAAACGGGGGTTACTTGAGAATAGAGAGAAGTGGCCACTCATTAAGTTCCTGAGCTAGGAAAGCACTTACAGCAGTGACAATTGTACATTTTTTTAGATCATCATATAGAAAGACTGAAGGAACAAATGGTAATTTCCATCTCTGAACCTTCTTCCTTTCAACC

mRNA sequence

ATGCCTTCATGGTGGGGGAAGCCATCATCCAAAGAAGTAAAGAAGAAAACGAGCAAGGAAAGTTTCATTGACTCATTACACCGTAAGTTTAAGAATTCACCTGAAGGTAAAGTAAGCAGTAGATCAGGAAGTTCTCGTAGACGTGGTGGTGACACTATATCTGAGAAGGGGTCTAAATCACCTCTATCAAGATCACCATCACCTTCCAAAGAAGTATCTAGATGTCAAAGTTTTGCTGAAAGATCACGTTCTCACCAACTTCCACTTCCAGAACTGCACCCTGTAGGTGTAGGTCGTACAGATTCTGGGATTAGTGTGACAGCAAAATCAAAATTAGAGAGAAGCTCCAAGACACCTTCATTCTTACCCCTCCCTAGACCTGCATGTATTCGAAGTAGGCCAGATCCTGCAGATTTGGATGGAGACTTGGTCACTGGTTCAGTCTCTGGTGAGTCCTCAAGTGATAGTGACGATCCCAATGATTCACGTCAACGTAGTCCTCAGGCAACTGACTATGACACTGGGACTAGAACTGTGATAGGTTCTACTGAACCTAGTGCAACTCTCAAAGATCAGTCTTCTACTGTCGTGCAAATAAATTTAAAAGAGGGCAAGAAATTGGAAAGTCTTTCATTTCCTCATAAGAATGCTTCATTACCTAAACGGAGGCCTTTGAGCAGCAATGTAACAAATCTACAAGTTCCTCGCCATGGGGCTTTCTTTAGCGCTCCAGATAGTTCCATGTCTAGTCCCTCTAGAAGTCCTATGAGGGTATTTAGCACTGAGCAAGTCATGAACACTGCTGTTTGGGCTGGAAAGTCTCACCCAGATGTCATTTTAGGTGGATCTGGCCACTGCTCCAGTCCTGGTTCTGGTCACAATTCAGGGCACAACTCTATGGGAGGTGATATGGCGGGCCACTTCTTTTGGCAACAAAGTAGGGGTAGCCCCGAATATTCTCCAGTACCTAGTTCTCGAATGACTAGCCCCGGCCCAAGCTCCAGAATTCAAAGTGGTGCTGTCACACCTATTCATCCTAGAGCAGGAGCTCCACCTGCTGAGTCACAAACATGCTGGCCTGATGAGAAACAAACTCATCGTTTGCCTCTTCCTCCCATAGCAATCTCAAACTCTTCTCCATTTTCTCATTCAAATTCAGCAGTGACATCTCCTTCAGTGCCTAGGAGTCCAGGAAGGACAGAGACTCCAGCAAGCCCAGGTTCACGCTGGAAAAAGGGGAAGCTATTGGGTCGGGGCACATTTGGGCACGTATATGTTGGTTTTAATAGGCTTATAGTCTTATCCGATATAAGTGATTATGATACCTTCATTATGGTATTGATTCCTGAGAGTGGAGAAATGTGTGCAATGAAGGAGGTCACGTTATTTTCTGACGATGCAAAGTCTAAGGAAAGTGCTAAACAATTAATGCAGGAAATTGCTTTATTGAGTCGTTTACGGCATCCCAACATCGTGCAGTATTATGGTTCGGAAACAGTGGGTGATAAACTCTATATATATCTGGAATATGTATCTGGTGGCTCCATTTATAAATTACTACAAGAGTATGGACAATTTGGGGAACTAGCAATTCGTAGCTATACTCAACAAATACTATCAGGACTTGCATATTTGCATGCTAAAACTACAGTGCACAGAATTGAACTTCTAACCTCTTCAGGGATATCAAAGGAGCAAACATACTTGTTGACCCAAATGGTCGTGTTAAATTGGCAGACTTTGGGATGGCGAAGCATATCACTGGCCAATCATGTCCCCTGTCGTTTAAGGGAAGCCCTTACTGGATGGCACCTGAGGTCAGAATTTATTGCATCTGTAAGCGAAAGCGATAAATATATGATGATACATATGCCTGACCCGTCACTTTCCCAGGTCATTAAGAACTCAAATGGTTGCAACCTTGCCGTTGATATTTGGAGTCTTGGATGTACTGTTCTGGAGATGGCTACTACAAAGCCACCTTGGAGCCAGTATGAAGGGGTTGCTGCGATGTTTAAGATTGGCAATAGCAAAGAACTCCCAGTAATTCCAGAGCACCTCTCAGACGATGGGAAGGATTTTGTGAGACTATGTCTGCAACGAAACCCACATCATCGACCTACAGCTGCTCAACTTTTGGATCATCCTTTTGTCAAATATGCTGCACCTGTAGAGAGGCCTTTTTTGAGCTCTGAACCTTCTGATGCAACTCCTGGGGTTACAAACGGAGTGAAGATTTTGGGTATTGGTCAATCACGGACTACTTCTTTGGACTCAGATAGCAGACTTGCAATTCATTCTTCAAGAGTCTCAAAAGCTGTTCTTCATGCCAGCGAAATCAGTATTCCCAGAAACATATCATGTCCTGTTTCACCAATTGGAAGTCCTCTTTTGCACTCGCGATCACCTCAACATCCCAGTGGAAGAATGTCGCCTTCACCTATATCAAGTCCACGAACCACTTCGGGCTCGTCAACGCCTTTGACAGGATGTGGTGGTGCAATTCCTTACAACCACCTGAAACAAGCTATATACTTGCAAGAGGGATTTGGAAGCATGCCAAAGTCCTTAAATAACAGTCCGTACAGCAGTGGTCTCTCCTTCCATGATTCAAATCCCGATATTTTTCGAGGGTTGCAGCCTGGGACTCCCATCTTCTCTGAAATGATACCAGAAAATGATATCTTGGGAAAGCAGGTTGGAAGGCCTGCTTATGGTGAATTGTATGATGGACAGCAGGTCTTGGCTGATCGAGTGTCTCGACAGCTCCTGAGGGATCATGTTAAAACAAACCCTTCTCTTGATTTGAGTCCTAGCCCTACATTGTCTGGCCGCATGAATGGTATCTGA

Coding sequence (CDS)

ATGCCTTCATGGTGGGGGAAGCCATCATCCAAAGAAGTAAAGAAGAAAACGAGCAAGGAAAGTTTCATTGACTCATTACACCGTAAGTTTAAGAATTCACCTGAAGGTAAAGTAAGCAGTAGATCAGGAAGTTCTCGTAGACGTGGTGGTGACACTATATCTGAGAAGGGGTCTAAATCACCTCTATCAAGATCACCATCACCTTCCAAAGAAGTATCTAGATGTCAAAGTTTTGCTGAAAGATCACGTTCTCACCAACTTCCACTTCCAGAACTGCACCCTGTAGGTGTAGGTCGTACAGATTCTGGGATTAGTGTGACAGCAAAATCAAAATTAGAGAGAAGCTCCAAGACACCTTCATTCTTACCCCTCCCTAGACCTGCATGTATTCGAAGTAGGCCAGATCCTGCAGATTTGGATGGAGACTTGGTCACTGGTTCAGTCTCTGGTGAGTCCTCAAGTGATAGTGACGATCCCAATGATTCACGTCAACGTAGTCCTCAGGCAACTGACTATGACACTGGGACTAGAACTGTGATAGGTTCTACTGAACCTAGTGCAACTCTCAAAGATCAGTCTTCTACTGTCGTGCAAATAAATTTAAAAGAGGGCAAGAAATTGGAAAGTCTTTCATTTCCTCATAAGAATGCTTCATTACCTAAACGGAGGCCTTTGAGCAGCAATGTAACAAATCTACAAGTTCCTCGCCATGGGGCTTTCTTTAGCGCTCCAGATAGTTCCATGTCTAGTCCCTCTAGAAGTCCTATGAGGGTATTTAGCACTGAGCAAGTCATGAACACTGCTGTTTGGGCTGGAAAGTCTCACCCAGATGTCATTTTAGGTGGATCTGGCCACTGCTCCAGTCCTGGTTCTGGTCACAATTCAGGGCACAACTCTATGGGAGGTGATATGGCGGGCCACTTCTTTTGGCAACAAAGTAGGGGTAGCCCCGAATATTCTCCAGTACCTAGTTCTCGAATGACTAGCCCCGGCCCAAGCTCCAGAATTCAAAGTGGTGCTGTCACACCTATTCATCCTAGAGCAGGAGCTCCACCTGCTGAGTCACAAACATGCTGGCCTGATGAGAAACAAACTCATCGTTTGCCTCTTCCTCCCATAGCAATCTCAAACTCTTCTCCATTTTCTCATTCAAATTCAGCAGTGACATCTCCTTCAGTGCCTAGGAGTCCAGGAAGGACAGAGACTCCAGCAAGCCCAGGTTCACGCTGGAAAAAGGGGAAGCTATTGGGTCGGGGCACATTTGGGCACGTATATGTTGGTTTTAATAGGCTTATAGTCTTATCCGATATAAGTGATTATGATACCTTCATTATGGTATTGATTCCTGAGAGTGGAGAAATGTGTGCAATGAAGGAGGTCACGTTATTTTCTGACGATGCAAAGTCTAAGGAAAGTGCTAAACAATTAATGCAGGAAATTGCTTTATTGAGTCGTTTACGGCATCCCAACATCGTGCAGTATTATGGTTCGGAAACAGTGGGTGATAAACTCTATATATATCTGGAATATGTATCTGGTGGCTCCATTTATAAATTACTACAAGAGTATGGACAATTTGGGGAACTAGCAATTCGTAGCTATACTCAACAAATACTATCAGGACTTGCATATTTGCATGCTAAAACTACAGTGCACAGAATTGAACTTCTAACCTCTTCAGGGATATCAAAGGAGCAAACATACTTGTTGACCCAAATGGTCGTGTTAAATTGGCAGACTTTGGGATGGCGAAGCATATCACTGGCCAATCATGTCCCCTGTCGTTTAAGGGAAGCCCTTACTGGATGGCACCTGAGGTCAGAATTTATTGCATCTGTAAGCGAAAGCGATAAATATATGATGATACATATGCCTGACCCGTCACTTTCCCAGGTCATTAAGAACTCAAATGGTTGCAACCTTGCCGTTGATATTTGGAGTCTTGGATGTACTGTTCTGGAGATGGCTACTACAAAGCCACCTTGGAGCCAGTATGAAGGGGTTGCTGCGATGTTTAAGATTGGCAATAGCAAAGAACTCCCAGTAATTCCAGAGCACCTCTCAGACGATGGGAAGGATTTTGTGAGACTATGTCTGCAACGAAACCCACATCATCGACCTACAGCTGCTCAACTTTTGGATCATCCTTTTGTCAAATATGCTGCACCTGTAGAGAGGCCTTTTTTGAGCTCTGAACCTTCTGATGCAACTCCTGGGGTTACAAACGGAGTGAAGATTTTGGGTATTGGTCAATCACGGACTACTTCTTTGGACTCAGATAGCAGACTTGCAATTCATTCTTCAAGAGTCTCAAAAGCTGTTCTTCATGCCAGCGAAATCAGTATTCCCAGAAACATATCATGTCCTGTTTCACCAATTGGAAGTCCTCTTTTGCACTCGCGATCACCTCAACATCCCAGTGGAAGAATGTCGCCTTCACCTATATCAAGTCCACGAACCACTTCGGGCTCGTCAACGCCTTTGACAGGATGTGGTGGTGCAATTCCTTACAACCACCTGAAACAAGCTATATACTTGCAAGAGGGATTTGGAAGCATGCCAAAGTCCTTAAATAACAGTCCGTACAGCAGTGGTCTCTCCTTCCATGATTCAAATCCCGATATTTTTCGAGGGTTGCAGCCTGGGACTCCCATCTTCTCTGAAATGATACCAGAAAATGATATCTTGGGAAAGCAGGTTGGAAGGCCTGCTTATGGTGAATTGTATGATGGACAGCAGGTCTTGGCTGATCGAGTGTCTCGACAGCTCCTGAGGGATCATGTTAAAACAAACCCTTCTCTTGATTTGAGTCCTAGCCCTACATTGTCTGGCCGCATGAATGGTATCTGA

Protein sequence

MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKSPLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPSFLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVIGSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAFFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWPDEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHLKQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI
Homology
BLAST of Spg002974.1 vs. NCBI nr
Match: KAG7013246.1 (Mitogen-activated protein kinase kinase kinase YODA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1552.3 bits (4018), Expect = 0.0e+00
Identity = 815/951 (85.70%), Postives = 838/951 (88.12%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSS RRGGDTISE   KS
Sbjct: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSCRRGGDTISE---KS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           PLSRSPSPSKEVSRCQSFA RS SHQLPLPELHP+G+G TDSGISVTAKSKLERSSKT S
Sbjct: 61  PLSRSPSPSKEVSRCQSFAGRSHSHQLPLPELHPIGIGPTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIR RPDPADLDGDLVTGSVSGESSSDSDDP DSRQRSPQATDYD GTRTV+
Sbjct: 121 FLPLPRPACIRRRPDPADLDGDLVTGSVSGESSSDSDDPIDSRQRSPQATDYDVGTRTVV 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GS EPSATLKDQSSTVVQ NLKEGKK ESLSFPHK  S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSIEPSATLKDQSSTVVQTNLKEGKKAESLSFPHKKPSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMRVFSTEQVMNTAVW+GKSH DVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWSGKSHSDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMA HFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAAHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE+VGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSESVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP+HRPTAA+LLDHPFVKY
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPYHRPTAARLLDHPFVKY 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSDATPGVTNGVK LGIGQSR TS+DSDSRLA+HS RVSK VLHAS+I
Sbjct: 721 AAPVERPILSSEPSDATPGVTNGVKTLGIGQSRITSVDSDSRLAVHSLRVSKPVLHASDI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLL SRSPQHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NILRNISCPVSPIGSPLLRSRSPQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQ- 900
           KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPG PIFSEMIPENDILGKQ 
Sbjct: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGAPIFSEMIPENDILGKQV 896

Query: 901 -VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
            VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPS TLSGRMNGI
Sbjct: 901 GVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSTTLSGRMNGI 896

BLAST of Spg002974.1 vs. NCBI nr
Match: KAG6574189.1 (Mitogen-activated protein kinase kinase kinase YODA, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 814/951 (85.59%), Postives = 837/951 (88.01%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSS RRGGDTISE   KS
Sbjct: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSCRRGGDTISE---KS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           PLSRSPSPSKEVSRCQSFA RS SHQLPLPELHP+G+G TDSGISVTAKSKLERSSKT S
Sbjct: 61  PLSRSPSPSKEVSRCQSFAGRSHSHQLPLPELHPIGIGPTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIR RPDPADLDGDLVTGSVSGESSSDSDDP DSRQRSPQATDYD GTRTV+
Sbjct: 121 FLPLPRPACIRRRPDPADLDGDLVTGSVSGESSSDSDDPIDSRQRSPQATDYDVGTRTVV 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GS EPSATLKDQSSTVVQ NLKEGKK ESLSFPHK  S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSIEPSATLKDQSSTVVQTNLKEGKKAESLSFPHKKPSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMRVFSTEQVMNTAVW+GKSH DVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWSGKSHSDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMA HFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAAHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE+V DKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSESVSDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP+HRPTAA+LLDHPFVKY
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPYHRPTAARLLDHPFVKY 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSDATPGVTNGVK LGIGQSR TS+DSDSRLA+HS RVSK VLHAS+I
Sbjct: 721 AAPVERPILSSEPSDATPGVTNGVKTLGIGQSRITSVDSDSRLAVHSLRVSKPVLHASDI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLL SRSPQHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NILRNISCPVSPIGSPLLRSRSPQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQ- 900
           KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPG PIFSEMIPENDILGKQ 
Sbjct: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGAPIFSEMIPENDILGKQV 896

Query: 901 -VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
            VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPS TLSGRMNGI
Sbjct: 901 GVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSTTLSGRMNGI 896

BLAST of Spg002974.1 vs. NCBI nr
Match: XP_022968042.1 (mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucurbita maxima] >XP_022968043.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucurbita maxima] >XP_022968044.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucurbita maxima] >XP_022968045.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucurbita maxima] >XP_022968046.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1549.3 bits (4010), Expect = 0.0e+00
Identity = 812/951 (85.38%), Postives = 838/951 (88.12%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSS RRGGDTISE   KS
Sbjct: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSCRRGGDTISE---KS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           PLSRSPSPSKEVSRCQSFAERS SHQLPLPELHP+G+G TDSGISVTAKSKLERSSKT S
Sbjct: 61  PLSRSPSPSKEVSRCQSFAERSHSHQLPLPELHPIGIGPTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACI+ RPDPADLDGDLVTGSVSGESSSD DDP DSRQRSPQATDY+ GTRTV+
Sbjct: 121 FLPLPRPACIQRRPDPADLDGDLVTGSVSGESSSDIDDPIDSRQRSPQATDYEVGTRTVV 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GS EPSATLKDQSSTV+Q NLKEGKK ESLSFPHK  S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSIEPSATLKDQSSTVMQTNLKEGKKAESLSFPHKKPSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMRVFSTEQVMNTAVW+GKSH DVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWSGKSHSDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMA HFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAAHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE+VGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSESVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP+HRPTAA+LLDHPFVKY
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPYHRPTAARLLDHPFVKY 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERPFLSSEPSDATPGVTNGVK LGIGQSR TS+DSD RLA+HSSRVSK VLHAS+I
Sbjct: 721 AAPVERPFLSSEPSDATPGVTNGVKTLGIGQSRITSVDSDGRLAVHSSRVSKPVLHASDI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLL SRSPQHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NILRNISCPVSPIGSPLLRSRSPQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQ- 900
           KQAIYLQEG GSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPG PIFSEMIPENDILGKQ 
Sbjct: 841 KQAIYLQEGLGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGAPIFSEMIPENDILGKQV 896

Query: 901 -VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
            VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPS TLSGRMNGI
Sbjct: 901 GVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSTTLSGRMNGI 896

BLAST of Spg002974.1 vs. NCBI nr
Match: XP_038884021.1 (mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Benincasa hispida] >XP_038884029.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Benincasa hispida] >XP_038884036.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Benincasa hispida] >XP_038884043.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1542.3 bits (3992), Expect = 0.0e+00
Identity = 803/949 (84.62%), Postives = 837/949 (88.20%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGK SSK+VKKKTSKESFI+SLHRKFKNSPEGKV+SRSGSSR+RGGDTISEKGSKS
Sbjct: 22  MPSWWGK-SSKDVKKKTSKESFIESLHRKFKNSPEGKVNSRSGSSRKRGGDTISEKGSKS 81

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           P+SRSPSPSKEV+RCQSFAERS SH+LPLP+LHPVGVGRTDSGISVTAKSKLERSSKT S
Sbjct: 82  PISRSPSPSKEVARCQSFAERSCSHKLPLPDLHPVGVGRTDSGISVTAKSKLERSSKTSS 141

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIRSRPDPADLDGDLVTGSV GESSSDSDDP DSRQRSP+ATDYD G RTVI
Sbjct: 142 FLPLPRPACIRSRPDPADLDGDLVTGSVFGESSSDSDDPTDSRQRSPRATDYDIGARTVI 201

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GSTEPS TLKDQS T VQINLKEGKK ESL FPHKN+S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 202 GSTEPSETLKDQSPTFVQINLKEGKKAESLPFPHKNSSMPKRRPLSSNVTNLQVPRHGAF 261

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMR+FSTEQVMN AVW GKSHPDVILGGSG CSSPGSGHNSGHNSM
Sbjct: 262 FSAPDSSMSSPSRSPMRIFSTEQVMNAAVWTGKSHPDVILGGSGPCSSPGSGHNSGHNSM 321

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPP ESQTC P
Sbjct: 322 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPTESQTCCP 381

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETP SPGSRWKKGKLLGRGT
Sbjct: 382 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPVSPGSRWKKGKLLGRGT 441

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 442 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 501

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE VGDK YIYLEYVSGGSIYKLLQEYGQFGELAIR+YTQQILS
Sbjct: 502 ALLSRLRHPNIVQYYGSEAVGDKFYIYLEYVSGGSIYKLLQEYGQFGELAIRNYTQQILS 561

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 562 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 621

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVD+WSLGCTVLEMATTKP
Sbjct: 622 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDVWSLGCTVLEMATTKP 681

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLL+HPFVK+
Sbjct: 682 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLEHPFVKH 741

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSD TPGVTNGVKILGIGQSRTTS+DSDSRLA+HSSRVSKAVLHASEI
Sbjct: 742 AAPVERPILSSEPSDTTPGVTNGVKILGIGQSRTTSMDSDSRLAVHSSRVSKAVLHASEI 801

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +IPRNISCPVSPIGSPLLHSRS QHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 802 NIPRNISCPVSPIGSPLLHSRSSQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 861

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQV 900
           KQAI+LQEGFGSMPKSLN+SP+S G+SFHDSNPDIFRGLQPG  IFSEMIPENDILGKQV
Sbjct: 862 KQAIFLQEGFGSMPKSLNSSPFSCGISFHDSNPDIFRGLQPGAHIFSEMIPENDILGKQV 917

Query: 901 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
           GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDL+PS TLS RMNGI
Sbjct: 922 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLNPSATLSSRMNGI 917

BLAST of Spg002974.1 vs. NCBI nr
Match: XP_038884051.1 (mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [Benincasa hispida] >XP_038884060.1 mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1542.3 bits (3992), Expect = 0.0e+00
Identity = 803/949 (84.62%), Postives = 837/949 (88.20%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGK SSK+VKKKTSKESFI+SLHRKFKNSPEGKV+SRSGSSR+RGGDTISEKGSKS
Sbjct: 1   MPSWWGK-SSKDVKKKTSKESFIESLHRKFKNSPEGKVNSRSGSSRKRGGDTISEKGSKS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           P+SRSPSPSKEV+RCQSFAERS SH+LPLP+LHPVGVGRTDSGISVTAKSKLERSSKT S
Sbjct: 61  PISRSPSPSKEVARCQSFAERSCSHKLPLPDLHPVGVGRTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIRSRPDPADLDGDLVTGSV GESSSDSDDP DSRQRSP+ATDYD G RTVI
Sbjct: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVFGESSSDSDDPTDSRQRSPRATDYDIGARTVI 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GSTEPS TLKDQS T VQINLKEGKK ESL FPHKN+S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSTEPSETLKDQSPTFVQINLKEGKKAESLPFPHKNSSMPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMR+FSTEQVMN AVW GKSHPDVILGGSG CSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRIFSTEQVMNAAVWTGKSHPDVILGGSGPCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPP ESQTC P
Sbjct: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPTESQTCCP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETP SPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPVSPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE VGDK YIYLEYVSGGSIYKLLQEYGQFGELAIR+YTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSEAVGDKFYIYLEYVSGGSIYKLLQEYGQFGELAIRNYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVD+WSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDVWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLL+HPFVK+
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLEHPFVKH 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSD TPGVTNGVKILGIGQSRTTS+DSDSRLA+HSSRVSKAVLHASEI
Sbjct: 721 AAPVERPILSSEPSDTTPGVTNGVKILGIGQSRTTSMDSDSRLAVHSSRVSKAVLHASEI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +IPRNISCPVSPIGSPLLHSRS QHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NIPRNISCPVSPIGSPLLHSRSSQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQV 900
           KQAI+LQEGFGSMPKSLN+SP+S G+SFHDSNPDIFRGLQPG  IFSEMIPENDILGKQV
Sbjct: 841 KQAIFLQEGFGSMPKSLNSSPFSCGISFHDSNPDIFRGLQPGAHIFSEMIPENDILGKQV 896

Query: 901 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
           GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDL+PS TLS RMNGI
Sbjct: 901 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLNPSATLSSRMNGI 896

BLAST of Spg002974.1 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 9.0e-229
Identity = 504/958 (52.61%), Postives = 612/958 (63.88%), Query Frame = 0

Query: 4   WWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKSPL- 63
           WW K  SK+ KKKT+KES ID+ +RK   + E + S RS  SRRR  + +SE+G+ S L 
Sbjct: 3   WWSK--SKDEKKKTNKESIIDAFNRKLGFASEDRSSGRSRKSRRRRDEIVSERGAISRLP 62

Query: 64  SRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPSFL 123
           SRSPSPS  VSRCQSFAERS +  LP P + P  V  TDSG++ + +  L+ + K PS+L
Sbjct: 63  SRSPSPSTRVSRCQSFAERSPAVPLPRPIVRP-HVTSTDSGMNGSQRPGLDANLK-PSWL 122

Query: 124 PLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVIGS 183
           PLP+P    S PD    + D  T SVS   SS  D P+DS   SP A+D + G RT +  
Sbjct: 123 PLPKPHGATSIPDNTGAEPDFATASVS-SGSSVGDIPSDS-LLSPLASDCENGNRTPV-- 182

Query: 184 TEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASL---PKRRPLSSNVTNLQVP-RHG 243
              + + +DQS   +  N    +  + +  P+KN  L   P+RRPL ++V NLQ+P R  
Sbjct: 183 ---NISSRDQS---MHSNKNSAEMFKPV--PNKNRILSASPRRRPLGTHVKNLQIPQRDL 242

Query: 244 AFFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHN 303
              SAPDS +SSPSRSPMR F  +QV N  +   K + DV L GSG CSSPGSG+NSG+N
Sbjct: 243 VLCSAPDSLLSSPSRSPMRSFIPDQVSNHGLLISKPYSDVSLLGSGQCSSPGSGYNSGNN 302

Query: 304 SMGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTC 363
           S+GGDMA   FW QSR SPE SPVPS RMTSPGPSSRIQSGAVTP+HPRAG     S T 
Sbjct: 303 SIGGDMATQLFWPQSRCSPECSPVPSPRMTSPGPSSRIQSGAVTPLHPRAGGSTTGSPTR 362

Query: 364 WPDE--KQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLL 423
             D+  +Q+HRLPLPP+ ISN+ PFS + SA TSPSVPRSP R E   SPGSRWKKG+LL
Sbjct: 363 RLDDNRQQSHRLPLPPLLISNTCPFSPTYSAATSPSVPRSPARAEATVSPGSRWKKGRLL 422

Query: 424 GRGTFGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQL 483
           G G+FGHVY+GFN                    ESGEMCAMKEVTL SDD KS+ESA+QL
Sbjct: 423 GMGSFGHVYLGFN-------------------SESGEMCAMKEVTLCSDDPKSRESAQQL 482

Query: 484 MQEIALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQ 543
            QEI++LSRLRH NIVQYYGSETV DKLYIYLEYVSGGSIYKLLQEYGQFGE AIR+YTQ
Sbjct: 483 GQEISVLSRLRHQNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYGQFGENAIRNYTQ 542

Query: 544 QILSGLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRL 603
           QILSGLAYLHAK TVHR                           +   +I +  H   ++
Sbjct: 543 QILSGLAYLHAKNTVHR--------------------------DIKGANILVDPHGRVKV 602

Query: 604 REALTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMA 663
            +     H+ ++      +   Y M         +VIKNSNG NLAVDIWSLGCTVLEMA
Sbjct: 603 ADFGMAKHITAQSGPLSFKGSPYWM-------APEVIKNSNGSNLAVDIWSLGCTVLEMA 662

Query: 664 TTKPPWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHP 723
           TTKPPWSQYEGV AMFKIGNSKELP IP+HLS++GKDFVR CLQRNP +RPTAAQLLDH 
Sbjct: 663 TTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHA 722

Query: 724 FVKYAAPVERPFLSSEPSDATPGVTNGVKILGIGQSRT-TSLDSDSRLAIHSSRVSKAVL 783
           FV+   P+ERP +S EP++A    ++ ++ L IG +R+   LDS+      ++   K + 
Sbjct: 723 FVRNVMPMERPIVSGEPAEAMNVASSTMRSLDIGHARSLPCLDSEDA----TNYQQKGLK 782

Query: 784 HASEISI---PRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCG 843
           H S  SI   PRN+SCP+SP+GSP+ HS SP H SGR SPSPISSP   SGSSTPLTGCG
Sbjct: 783 HGSGFSISQSPRNMSCPISPVGSPIFHSHSP-HISGRRSPSPISSPHALSGSSTPLTGCG 842

Query: 844 GAIPYNHLKQAI--YLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRG-LQPGTPIFSEM 903
           GAIP++H +Q    +L EG GS     +   + +   F + +    R    P TP     
Sbjct: 843 GAIPFHHQRQTTVNFLHEGIGSSRSPGSGGNFYTNSFFQEPSRQQDRSRSSPRTPPHVFW 880

Query: 904 IPENDILGKQVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMN 948
                I      +P Y    D Q VL+D VS+QLL +H+K   SLDL P  +  G  N
Sbjct: 903 DNNGSI------QPGYNWNKDNQPVLSDHVSQQLLSEHLKLK-SLDLRPGFSTPGSTN 880

BLAST of Spg002974.1 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.3e-75
Identity = 218/548 (39.78%), Postives = 280/548 (51.09%), Query Frame = 0

Query: 275 HPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEY--SPVPSSRMTSPGP 334
           H D + G +   +S  S  +SG      D        + RG  ++  +  P S       
Sbjct: 86  HQDHVSGSTSGSTSVSSVSSSG----SADDQSQLVASRGRGDVKFNVAAAPRSPERVSPK 145

Query: 335 SSRIQSGAVTPIHPR-AGAPPAESQTCWPDE----KQTHRLPLPPIAISNSSPFSHSNSA 394
           ++ I +   +P H R +G    ES T   D+     + H LP PP     +SP       
Sbjct: 146 AATITTRPTSPRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP-----TSP------- 205

Query: 395 VTSPSV---PRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFI 454
            TSPS     R  G  ET  S  S WKKGK LG GTFG VY+GFN               
Sbjct: 206 -TSPSAVHGSRIGGGYETSPSGFSTWKKGKFLGSGTFGQVYLGFN--------------- 265

Query: 455 MVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKL 514
                E G+MCA+KEV + SDD  SKE  KQL QEI LL++L HPNIVQYYGSE   + L
Sbjct: 266 ----SEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETL 325

Query: 515 YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISK 574
            +YLEYVSGGSI+KLL++YG F E  I++YT+QIL+GLAYLH + TVHR          K
Sbjct: 326 SVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHR--------DIK 385

Query: 575 EQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHM 634
               L+     +     G     +A HV        T +     F  S            
Sbjct: 386 GANILVDPNGEIKLADFG-----MAKHV--------TAFSTMLSFKGS------------ 445

Query: 635 PDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIP 694
           P     +V+ + NG   AVDIWSLGCT+LEMAT+KPPWSQ+EGVAA+FKIGNSK+ P IP
Sbjct: 446 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIP 505

Query: 695 EHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNGV 754
           +HLS+D K+F+RLCLQRNP  RPTA+QLL+HPF++    V     +S P D  P   +G 
Sbjct: 506 DHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVAS---TSLPKDFPPRSYDGN 558

Query: 755 KILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSPQ 813
             L   +       S    A     +S+ +   S  ++    S PVSP  SP L    P 
Sbjct: 566 FSLQPTREPYPGRLSHDNYA--KQPLSRTIKSPSRENVRAITSLPVSPCSSP-LRQLGPA 558

BLAST of Spg002974.1 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 8.3e-65
Identity = 211/556 (37.95%), Postives = 284/556 (51.08%), Query Frame = 0

Query: 184 EPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAFFSA 243
           +P   + D++S+   +    G          +N+S     P + N   + +P      SA
Sbjct: 131 DPERLISDRTSSGPPLTSVNGGFARDSRKATENSSYQDFSPRNRNGYWVNIPT----MSA 190

Query: 244 PDSSMSSPSRSPMRVFSTEQV-------MNTAVWAGKSHPDVILGGSGHCSSPGSGHN-- 303
           P S   SP  SP R  +   +        +   W   S PD+ L  SG    P + ++  
Sbjct: 191 PTSPYMSPVPSPQRKSTGHDLPFFYLPPKSNQAW---SAPDMPLDTSG--LPPPAFYDIT 250

Query: 304 --SGHNSMGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPP 363
             S  NS           +Q R SP+ S  PSS + S   S+  +    +P+HPR     
Sbjct: 251 AFSTDNSPIHSPQPRSPRKQIR-SPQPSR-PSSPLHSVDSSAPPRDSVSSPLHPRLSTDV 310

Query: 364 AESQTCWPDEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKK 423
              +    D    H LPLPP A  +SS      SA + PS P++P + ++     S+WKK
Sbjct: 311 TNGRR---DCCNVHPLPLPPGATCSSS------SAASVPS-PQAPLKLDS-FPMNSQWKK 370

Query: 424 GKLLGRGTFGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKES 483
           GKL+GRGTFG VYV  N                    E+G +CAMKEV LF DD KS E 
Sbjct: 371 GKLIGRGTFGSVYVASN-------------------SETGALCAMKEVELFPDDPKSAEC 430

Query: 484 AKQLMQEIALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEY-GQFGELAI 543
            KQL QEI LLS L+HPNIVQY+GSETV D+ +IYLEYV  GSI K ++++ G   E  +
Sbjct: 431 IKQLEQEIKLLSNLQHPNIVQYFGSETVEDRFFIYLEYVHPGSINKYIRDHCGTMTESVV 490

Query: 544 RSYTQQILSGLAYLHAKTTVHR-----IELLTSSGISKEQTYLLTQMVVLNWQTLGWRSI 603
           R++T+ ILSGLAYLH K TVHR       L+ +SG+ K   +                  
Sbjct: 491 RNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASGVVKLADF------------------ 550

Query: 604 SLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVI--KNSN-GCNLAV 663
            +A H        LTG   R++   S+  S  +M      P L Q +  K+SN     AV
Sbjct: 551 GMAKH--------LTG--QRADL--SLKGSPYWMA-----PELMQAVMQKDSNPDLAFAV 608

Query: 664 DIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP 720
           DIWSLGCT++EM T KPPWS++EG AAMFK+   ++ P IPE +S +GKDF+RLC QRNP
Sbjct: 611 DIWSLGCTIIEMFTGKPPWSEFEGAAAMFKV--MRDSPPIPESMSPEGKDFLRLCFQRNP 608

BLAST of Spg002974.1 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 2.4e-48
Identity = 126/354 (35.59%), Postives = 178/354 (50.28%), Query Frame = 0

Query: 387 AVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFIMV 446
           A+ S S    P +     +P  RW+KG+++G G FG VY+G N                 
Sbjct: 57  ALPSISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMN----------------- 116

Query: 447 LIPESGEMCAMKEVTLFSDDA---KSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDK 506
              +SGE+ A+KEV++  + A   +++   ++L +E+ LL  L HPNIV+Y G+      
Sbjct: 117 --VDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGS 176

Query: 507 LYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHR-----IELLT 566
           L I LE+V GGSI  LL ++G F E  IR YT+Q+L GL YLH    +HR       L+ 
Sbjct: 177 LNILLEFVPGGSISSLLGKFGSFPESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVD 236

Query: 567 SSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDK 626
           + G  K   +  ++ VV            LA     +  +    W               
Sbjct: 237 NKGCIKLADFGASKKVV-----------ELATMTGAKSMKGTPYW--------------- 296

Query: 627 YMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWS-QYEGVAAMFKIGNS 686
                     ++  +    G + + DIWS+GCT++EMAT KPPWS QY+ VAA+F IG +
Sbjct: 297 ----------MAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTT 355

Query: 687 KELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSS 732
           K  P IPEHLS + KDF+  CLQ+ PH R +A+ LL HPFV       RPFL S
Sbjct: 357 KSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRS 355

BLAST of Spg002974.1 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 3.0e-46
Identity = 123/367 (33.51%), Postives = 179/367 (48.77%), Query Frame = 0

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           D+ Q ++ P P +     +     +     PS    P       +P   W+KG+L+GRG 
Sbjct: 20  DDNQENQPPFPGVLADKITSCIRKSKIFIKPSFSPPPPANTVDMAPPISWRKGQLIGRGA 79

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTL---FSDDAKSKESAKQLM 480
           FG VY+G N                    +SGE+ A+K+V +   F+   K++   ++L 
Sbjct: 80  FGTVYMGMNL-------------------DSGELLAVKQVLIAANFASKEKTQAHIQELE 139

Query: 481 QEIALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQ 540
           +E+ LL  L HPNIV+Y G+    D L I LE+V GGSI  LL+++G F E  +R+YT+Q
Sbjct: 140 EEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQ 199

Query: 541 ILSGLAYLHAKTTVHR-----IELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHV 600
           +L GL YLH    +HR       L+ + G  K   +  ++ V             LA   
Sbjct: 200 LLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVA-----------ELATMT 259

Query: 601 PCRLREALTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTV 660
             +  +    W                         ++  +    G + + DIWS+GCTV
Sbjct: 260 GAKSMKGTPYW-------------------------MAPEVILQTGHSFSADIWSVGCTV 319

Query: 661 LEMATTKPPWS-QYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQ 719
           +EM T K PWS QY+ VAA+F IG +K  P IP+ LS D KDF+  CLQ  P+ RPTA++
Sbjct: 320 IEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASE 331

BLAST of Spg002974.1 vs. ExPASy TrEMBL
Match: A0A6J1HW31 (mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467405 PE=4 SV=1)

HSP 1 Score: 1549.3 bits (4010), Expect = 0.0e+00
Identity = 812/951 (85.38%), Postives = 838/951 (88.12%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSS RRGGDTISE   KS
Sbjct: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSCRRGGDTISE---KS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           PLSRSPSPSKEVSRCQSFAERS SHQLPLPELHP+G+G TDSGISVTAKSKLERSSKT S
Sbjct: 61  PLSRSPSPSKEVSRCQSFAERSHSHQLPLPELHPIGIGPTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACI+ RPDPADLDGDLVTGSVSGESSSD DDP DSRQRSPQATDY+ GTRTV+
Sbjct: 121 FLPLPRPACIQRRPDPADLDGDLVTGSVSGESSSDIDDPIDSRQRSPQATDYEVGTRTVV 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GS EPSATLKDQSSTV+Q NLKEGKK ESLSFPHK  S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSIEPSATLKDQSSTVMQTNLKEGKKAESLSFPHKKPSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMRVFSTEQVMNTAVW+GKSH DVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWSGKSHSDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMA HFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAAHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE+VGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSESVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP+HRPTAA+LLDHPFVKY
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPYHRPTAARLLDHPFVKY 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERPFLSSEPSDATPGVTNGVK LGIGQSR TS+DSD RLA+HSSRVSK VLHAS+I
Sbjct: 721 AAPVERPFLSSEPSDATPGVTNGVKTLGIGQSRITSVDSDGRLAVHSSRVSKPVLHASDI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLL SRSPQHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NILRNISCPVSPIGSPLLRSRSPQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQ- 900
           KQAIYLQEG GSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPG PIFSEMIPENDILGKQ 
Sbjct: 841 KQAIYLQEGLGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGAPIFSEMIPENDILGKQV 896

Query: 901 -VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
            VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPS TLSGRMNGI
Sbjct: 901 GVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSTTLSGRMNGI 896

BLAST of Spg002974.1 vs. ExPASy TrEMBL
Match: A0A6J1FZV1 (mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449425 PE=4 SV=1)

HSP 1 Score: 1541.6 bits (3990), Expect = 0.0e+00
Identity = 811/951 (85.28%), Postives = 835/951 (87.80%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSS RRGGDTISE   KS
Sbjct: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSCRRGGDTISE---KS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           PLSRSPSPSKEVSRCQSFA RS SHQLPLPELHP+G+G TDSGISVTAKSKLERSSKT S
Sbjct: 61  PLSRSPSPSKEVSRCQSFAGRSHSHQLPLPELHPIGIGPTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIR RPDPADLDGDLVTGSVSGESSSDSDDP DSRQRSPQATDYD GTRTV+
Sbjct: 121 FLPLPRPACIRRRPDPADLDGDLVTGSVSGESSSDSDDPIDSRQRSPQATDYDVGTRTVV 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GS EPSATLKDQSSTVVQ NLKEGKK ESLSFPHK  S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSIEPSATLKDQSSTVVQTNLKEGKKAESLSFPHKKPSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMRVFSTEQVMNTAVW+GKSH DVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWSGKSHSDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMA HFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAAHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAISNSSPFSHS SAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISNSSPFSHSTSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSE+VGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSESVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAKTTVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNP+HRPTAA+LLDHPFVKY
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPYHRPTAARLLDHPFVKY 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSDATPGVTNGVK LGIGQSR TS+DSDSRLA+HS RVSK VLHAS+I
Sbjct: 721 AAPVERPILSSEPSDATPGVTNGVKTLGIGQSRITSVDSDSRLAVHSLRVSKPVLHASDI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLL SRSPQHPSGRMSPSPISSPRTTSGSSTPLT CGGAIPYNHL
Sbjct: 781 NILRNISCPVSPIGSPLLRSRSPQHPSGRMSPSPISSPRTTSGSSTPLTACGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQ- 900
           KQAIYLQ+GFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQ G PIFSEMIPENDILGKQ 
Sbjct: 841 KQAIYLQDGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQHGAPIFSEMIPENDILGKQV 896

Query: 901 -VGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
            VGRPAYGELYDGQQVLADRVSRQLLRDHVKTN SLDLSPS TLSGRMNGI
Sbjct: 901 GVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNLSLDLSPSTTLSGRMNGI 896

BLAST of Spg002974.1 vs. ExPASy TrEMBL
Match: A0A6J1CGN4 (mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia OX=3673 GN=LOC111011495 PE=4 SV=1)

HSP 1 Score: 1539.2 bits (3984), Expect = 0.0e+00
Identity = 808/951 (84.96%), Postives = 837/951 (88.01%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRR-GGDTISEKGSK 60
           MPSWWGK SSKEVKKKTSKESFIDSLHRKFKNSPEGKV+ RSG+SRRR G DTISEKGSK
Sbjct: 1   MPSWWGKSSSKEVKKKTSKESFIDSLHRKFKNSPEGKVNGRSGTSRRRCGADTISEKGSK 60

Query: 61  SPLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTP 120
           SPLS SPSPSKEVSRCQSFAERSRSHQLPLP  HPVGVGRTDSGISVTAK KLERSSKT 
Sbjct: 61  SPLSGSPSPSKEVSRCQSFAERSRSHQLPLPHQHPVGVGRTDSGISVTAKLKLERSSKTS 120

Query: 121 SFLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTV 180
           SFLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDS+DPNDSRQRSPQATDYD GTRTV
Sbjct: 121 SFLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSEDPNDSRQRSPQATDYDIGTRTV 180

Query: 181 IGSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGA 240
            GSTEP ATLKDQS TV+QINL+EGK+ E+LS  HKN+SLPKRRPLSSNVTNLQVPRHGA
Sbjct: 181 TGSTEPCATLKDQSPTVLQINLREGKQAETLSLSHKNSSLPKRRPLSSNVTNLQVPRHGA 240

Query: 241 FFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNS 300
           FFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGK+HPDVILGGSGHCSSPGSGHNSGHNS
Sbjct: 241 FFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKAHPDVILGGSGHCSSPGSGHNSGHNS 300

Query: 301 MGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCW 360
           MGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCW
Sbjct: 301 MGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCW 360

Query: 361 PDEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRG 420
           PDEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRG
Sbjct: 361 PDEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRG 420

Query: 421 TFGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQE 480
           TFGHVYVGFN                    ESGEMCAMKEVTLFSDDAKSKESAKQLMQE
Sbjct: 421 TFGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSKESAKQLMQE 480

Query: 481 IALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQIL 540
           IALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQI+
Sbjct: 481 IALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQII 540

Query: 541 SGLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREA 600
           SGLAYLHAKTTVHR                           +   +I +  +   +L + 
Sbjct: 541 SGLAYLHAKTTVHR--------------------------DIKGANILVDPNGRVKLADF 600

Query: 601 LTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTK 660
               H+  +      +   Y M         +VIKNSNGCNLAVDIWSLGCTVLEMATTK
Sbjct: 601 GMAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDIWSLGCTVLEMATTK 660

Query: 661 PPWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVK 720
           PPWSQYEGVAAMFKIGNSKELPVIP+HLSDDGKDFVR CLQRNP HRPTAAQLL+HPF+K
Sbjct: 661 PPWSQYEGVAAMFKIGNSKELPVIPDHLSDDGKDFVRQCLQRNPLHRPTAAQLLEHPFIK 720

Query: 721 YAAPVERPFLSSEPSDATPGVTNGVKILGIGQSR-TTSLDSDSRLAIHSSRVSKAVLHAS 780
           YAAPVERP LSSEPSDATPGVTNGVK LGIGQSR  T +DSD RLA+HSSRVSKAVLHAS
Sbjct: 721 YAAPVERPILSSEPSDATPGVTNGVKTLGIGQSRIPTRVDSD-RLAVHSSRVSKAVLHAS 780

Query: 781 EISIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYN 840
           EI+IPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYN
Sbjct: 781 EINIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYN 840

Query: 841 HLKQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGK 900
           HLKQ+IYLQEGFGSMPKS+NNSPYSSGLSFHDSNPDIFRGLQPG  IFSE+IPENDILGK
Sbjct: 841 HLKQSIYLQEGFGSMPKSINNSPYSSGLSFHDSNPDIFRGLQPGAHIFSELIPENDILGK 898

Query: 901 QVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
           QVGR AYG  YDGQ VLADRVSRQLLRDHVKTNPSLDLSPSP LSGRMNGI
Sbjct: 901 QVGRSAYGASYDGQLVLADRVSRQLLRDHVKTNPSLDLSPSPALSGRMNGI 898

BLAST of Spg002974.1 vs. ExPASy TrEMBL
Match: A0A0A0L9U6 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182770 PE=4 SV=1)

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 796/949 (83.88%), Postives = 832/949 (87.67%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGK SSK+VKKKTSKESFIDSLHRKFKNSPEGKV+SRSGSSR+RGGDT+SEKGSKS
Sbjct: 1   MPSWWGK-SSKDVKKKTSKESFIDSLHRKFKNSPEGKVNSRSGSSRKRGGDTVSEKGSKS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           P+SRSPSPSKEV+RCQSFAER+ SH+LPLP+L PVGVGRTDSGISV AKSKLERSSKT S
Sbjct: 61  PISRSPSPSKEVARCQSFAERTHSHKLPLPDLRPVGVGRTDSGISVAAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIRSRPDPADLDGDLVTGSV GESSSDSDDPNDSRQRSP ATDYD G RTVI
Sbjct: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVFGESSSDSDDPNDSRQRSPPATDYDIGARTVI 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GSTEPS TLKDQS TVVQ NLKEGKK ESL FPHKN+S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSTEPSETLKDQSPTVVQKNLKEGKKAESLPFPHKNSSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMR+FSTEQVMN AVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRIFSTEQVMNAAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAIS  SPFSHSNSAVTSPSVPRSPGRTETPASPG RWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISICSPFSHSNSAVTSPSVPRSPGRTETPASPGPRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKS+ESAKQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSRESAKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSETVGDK YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSETVGDKFYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAK TVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKATVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVD+WSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDVWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLL+HPFVK+
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLEHPFVKH 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP L SEPSD TPGVTNGVKILGIGQSRTTS+DSD RLA+HSSRVSKAVLHASEI
Sbjct: 721 AAPVERPILISEPSDTTPGVTNGVKILGIGQSRTTSMDSDGRLAVHSSRVSKAVLHASEI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRT SGSSTPLTGCGGAIPYNHL
Sbjct: 781 NISRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTMSGSSTPLTGCGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQV 900
           KQ IYLQEGF SMPKSLN+SPYSSG+SFHDSNPDIFRGLQPG  IFSEMIPEN++LGKQ+
Sbjct: 841 KQTIYLQEGFVSMPKSLNSSPYSSGISFHDSNPDIFRGLQPGAHIFSEMIPENEVLGKQI 896

Query: 901 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
           GRPAY E+YDGQ +LADRVSRQLLRDHVK NPSLDLSPS TLSGRMNGI
Sbjct: 901 GRPAYSEVYDGQHILADRVSRQLLRDHVKANPSLDLSPSATLSGRMNGI 896

BLAST of Spg002974.1 vs. ExPASy TrEMBL
Match: A0A5D3DHE5 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00770 PE=4 SV=1)

HSP 1 Score: 1526.5 bits (3951), Expect = 0.0e+00
Identity = 795/949 (83.77%), Postives = 832/949 (87.67%), Query Frame = 0

Query: 1   MPSWWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKS 60
           MPSWWGK SSK+VKKKTSKESFIDSLHRKF+NSP+GKV+SRSGSSR+RGGDT+SEKGSKS
Sbjct: 1   MPSWWGK-SSKDVKKKTSKESFIDSLHRKFRNSPDGKVNSRSGSSRKRGGDTVSEKGSKS 60

Query: 61  PLSRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPS 120
           P+SRSPSPSKEV RCQSFAERS SH+LPLP+L PVGVGRTDSGISVTAKSKLERSSKT S
Sbjct: 61  PISRSPSPSKEVVRCQSFAERSHSHKLPLPDLRPVGVGRTDSGISVTAKSKLERSSKTSS 120

Query: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVI 180
           FLPLPRPACIRSRPDPADLDGDLVTGSV GESSSDSDDPNDSRQRSP ATDYD G  TVI
Sbjct: 121 FLPLPRPACIRSRPDPADLDGDLVTGSVFGESSSDSDDPNDSRQRSPPATDYDIGAITVI 180

Query: 181 GSTEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASLPKRRPLSSNVTNLQVPRHGAF 240
           GSTEPS TLKDQS TVVQINLKEGKK E+L FPHK++S+PKRRPLSSNVTNLQVPRHGAF
Sbjct: 181 GSTEPSETLKDQSPTVVQINLKEGKKAENLPFPHKHSSIPKRRPLSSNVTNLQVPRHGAF 240

Query: 241 FSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300
           FSAPDSSMSSPSRSPMR+FSTEQVMN AVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM
Sbjct: 241 FSAPDSSMSSPSRSPMRIFSTEQVMNAAVWAGKSHPDVILGGSGHCSSPGSGHNSGHNSM 300

Query: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360
           GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP
Sbjct: 301 GGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTCWP 360

Query: 361 DEKQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLLGRGT 420
           DEKQTHRLPLPPIAIS SSPFSHSNSAVTSPSVPRSPGRT+TPASPG RWKKGKLLGRGT
Sbjct: 361 DEKQTHRLPLPPIAISISSPFSHSNSAVTSPSVPRSPGRTDTPASPGPRWKKGKLLGRGT 420

Query: 421 FGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEI 480
           FGHVYVGFN                    ESGEMCAMKEVTLFSDDAKS+ES KQLMQEI
Sbjct: 421 FGHVYVGFN-------------------SESGEMCAMKEVTLFSDDAKSRESTKQLMQEI 480

Query: 481 ALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540
           ALLSRLRHPNIVQYYGSETVGDK YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS
Sbjct: 481 ALLSRLRHPNIVQYYGSETVGDKFYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILS 540

Query: 541 GLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREAL 600
           GLAYLHAK TVHR                           +   +I +  +   +L +  
Sbjct: 541 GLAYLHAKATVHR--------------------------DIKGANILVDPNGRVKLADFG 600

Query: 601 TGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKP 660
              H+  +      +   Y M         +VIKNSNGCNLAVD+WSLGCTVLEMATTKP
Sbjct: 601 MAKHITGQSCPLSFKGSPYWM-------APEVIKNSNGCNLAVDVWSLGCTVLEMATTKP 660

Query: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKY 720
           PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLL+HPFVK+
Sbjct: 661 PWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLEHPFVKH 720

Query: 721 AAPVERPFLSSEPSDATPGVTNGVKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEI 780
           AAPVERP LSSEPSD TPGVTNGVKILGI QSRTTS+DSD RLA+HSSRVSKAVLHASEI
Sbjct: 721 AAPVERPILSSEPSDTTPGVTNGVKILGISQSRTTSMDSDGRLAVHSSRVSKAVLHASEI 780

Query: 781 SIPRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCGGAIPYNHL 840
           +I RNISCPVSPIGSPLLHSRS QHPSGRMSPSPISSPRT SGSSTPLTGCGGAIPYNHL
Sbjct: 781 NISRNISCPVSPIGSPLLHSRS-QHPSGRMSPSPISSPRTMSGSSTPLTGCGGAIPYNHL 840

Query: 841 KQAIYLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRGLQPGTPIFSEMIPENDILGKQV 900
           KQ IYLQEGF SMPKSLN+SPYSSG+SFHDSNPDIFRGLQPG  IFSEMIPEND+LGKQ+
Sbjct: 841 KQTIYLQEGFLSMPKSLNSSPYSSGISFHDSNPDIFRGLQPGAHIFSEMIPENDVLGKQI 895

Query: 901 GRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMNGI 950
           GRPAY ELYDGQ VLADRVS+QLLRDHVKTNPSLDLSPS TLSGRMNGI
Sbjct: 901 GRPAYSELYDGQHVLADRVSQQLLRDHVKTNPSLDLSPSATLSGRMNGI 895

BLAST of Spg002974.1 vs. TAIR 10
Match: AT1G63700.1 (Protein kinase superfamily protein )

HSP 1 Score: 795.0 bits (2052), Expect = 6.4e-230
Identity = 504/958 (52.61%), Postives = 612/958 (63.88%), Query Frame = 0

Query: 4   WWGKPSSKEVKKKTSKESFIDSLHRKFKNSPEGKVSSRSGSSRRRGGDTISEKGSKSPL- 63
           WW K  SK+ KKKT+KES ID+ +RK   + E + S RS  SRRR  + +SE+G+ S L 
Sbjct: 3   WWSK--SKDEKKKTNKESIIDAFNRKLGFASEDRSSGRSRKSRRRRDEIVSERGAISRLP 62

Query: 64  SRSPSPSKEVSRCQSFAERSRSHQLPLPELHPVGVGRTDSGISVTAKSKLERSSKTPSFL 123
           SRSPSPS  VSRCQSFAERS +  LP P + P  V  TDSG++ + +  L+ + K PS+L
Sbjct: 63  SRSPSPSTRVSRCQSFAERSPAVPLPRPIVRP-HVTSTDSGMNGSQRPGLDANLK-PSWL 122

Query: 124 PLPRPACIRSRPDPADLDGDLVTGSVSGESSSDSDDPNDSRQRSPQATDYDTGTRTVIGS 183
           PLP+P    S PD    + D  T SVS   SS  D P+DS   SP A+D + G RT +  
Sbjct: 123 PLPKPHGATSIPDNTGAEPDFATASVS-SGSSVGDIPSDS-LLSPLASDCENGNRTPV-- 182

Query: 184 TEPSATLKDQSSTVVQINLKEGKKLESLSFPHKNASL---PKRRPLSSNVTNLQVP-RHG 243
              + + +DQS   +  N    +  + +  P+KN  L   P+RRPL ++V NLQ+P R  
Sbjct: 183 ---NISSRDQS---MHSNKNSAEMFKPV--PNKNRILSASPRRRPLGTHVKNLQIPQRDL 242

Query: 244 AFFSAPDSSMSSPSRSPMRVFSTEQVMNTAVWAGKSHPDVILGGSGHCSSPGSGHNSGHN 303
              SAPDS +SSPSRSPMR F  +QV N  +   K + DV L GSG CSSPGSG+NSG+N
Sbjct: 243 VLCSAPDSLLSSPSRSPMRSFIPDQVSNHGLLISKPYSDVSLLGSGQCSSPGSGYNSGNN 302

Query: 304 SMGGDMAGHFFWQQSRGSPEYSPVPSSRMTSPGPSSRIQSGAVTPIHPRAGAPPAESQTC 363
           S+GGDMA   FW QSR SPE SPVPS RMTSPGPSSRIQSGAVTP+HPRAG     S T 
Sbjct: 303 SIGGDMATQLFWPQSRCSPECSPVPSPRMTSPGPSSRIQSGAVTPLHPRAGGSTTGSPTR 362

Query: 364 WPDE--KQTHRLPLPPIAISNSSPFSHSNSAVTSPSVPRSPGRTETPASPGSRWKKGKLL 423
             D+  +Q+HRLPLPP+ ISN+ PFS + SA TSPSVPRSP R E   SPGSRWKKG+LL
Sbjct: 363 RLDDNRQQSHRLPLPPLLISNTCPFSPTYSAATSPSVPRSPARAEATVSPGSRWKKGRLL 422

Query: 424 GRGTFGHVYVGFNRLIVLSDISDYDTFIMVLIPESGEMCAMKEVTLFSDDAKSKESAKQL 483
           G G+FGHVY+GFN                    ESGEMCAMKEVTL SDD KS+ESA+QL
Sbjct: 423 GMGSFGHVYLGFN-------------------SESGEMCAMKEVTLCSDDPKSRESAQQL 482

Query: 484 MQEIALLSRLRHPNIVQYYGSETVGDKLYIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQ 543
            QEI++LSRLRH NIVQYYGSETV DKLYIYLEYVSGGSIYKLLQEYGQFGE AIR+YTQ
Sbjct: 483 GQEISVLSRLRHQNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYGQFGENAIRNYTQ 542

Query: 544 QILSGLAYLHAKTTVHRIELLTSSGISKEQTYLLTQMVVLNWQTLGWRSISLANHVPCRL 603
           QILSGLAYLHAK TVHR                           +   +I +  H   ++
Sbjct: 543 QILSGLAYLHAKNTVHR--------------------------DIKGANILVDPHGRVKV 602

Query: 604 REALTGWHLRSEFIASVSESDKYMMIHMPDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMA 663
            +     H+ ++      +   Y M         +VIKNSNG NLAVDIWSLGCTVLEMA
Sbjct: 603 ADFGMAKHITAQSGPLSFKGSPYWM-------APEVIKNSNGSNLAVDIWSLGCTVLEMA 662

Query: 664 TTKPPWSQYEGVAAMFKIGNSKELPVIPEHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHP 723
           TTKPPWSQYEGV AMFKIGNSKELP IP+HLS++GKDFVR CLQRNP +RPTAAQLLDH 
Sbjct: 663 TTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHA 722

Query: 724 FVKYAAPVERPFLSSEPSDATPGVTNGVKILGIGQSRT-TSLDSDSRLAIHSSRVSKAVL 783
           FV+   P+ERP +S EP++A    ++ ++ L IG +R+   LDS+      ++   K + 
Sbjct: 723 FVRNVMPMERPIVSGEPAEAMNVASSTMRSLDIGHARSLPCLDSEDA----TNYQQKGLK 782

Query: 784 HASEISI---PRNISCPVSPIGSPLLHSRSPQHPSGRMSPSPISSPRTTSGSSTPLTGCG 843
           H S  SI   PRN+SCP+SP+GSP+ HS SP H SGR SPSPISSP   SGSSTPLTGCG
Sbjct: 783 HGSGFSISQSPRNMSCPISPVGSPIFHSHSP-HISGRRSPSPISSPHALSGSSTPLTGCG 842

Query: 844 GAIPYNHLKQAI--YLQEGFGSMPKSLNNSPYSSGLSFHDSNPDIFRG-LQPGTPIFSEM 903
           GAIP++H +Q    +L EG GS     +   + +   F + +    R    P TP     
Sbjct: 843 GAIPFHHQRQTTVNFLHEGIGSSRSPGSGGNFYTNSFFQEPSRQQDRSRSSPRTPPHVFW 880

Query: 904 IPENDILGKQVGRPAYGELYDGQQVLADRVSRQLLRDHVKTNPSLDLSPSPTLSGRMN 948
                I      +P Y    D Q VL+D VS+QLL +H+K   SLDL P  +  G  N
Sbjct: 903 DNNGSI------QPGYNWNKDNQPVLSDHVSQQLLSEHLKLK-SLDLRPGFSTPGSTN 880

BLAST of Spg002974.1 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 288.1 bits (736), Expect = 2.6e-77
Identity = 218/548 (39.78%), Postives = 281/548 (51.28%), Query Frame = 0

Query: 275 HPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEY--SPVPSSRMTSPGP 334
           H D + G +   +S  S  +SG      D        + RG  ++  +  P S       
Sbjct: 86  HQDHVSGSTSGSTSVSSVSSSG----SADDQSQLVASRGRGDVKFNVAAAPRSPERVSPK 145

Query: 335 SSRIQSGAVTPIHPR-AGAPPAESQTCWPDE----KQTHRLPLPPIAISNSSPFSHSNSA 394
           ++ I +   +P H R +G    ES T   D+     + H LP PP     +SP       
Sbjct: 146 AATITTRPTSPRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP-----TSP------- 205

Query: 395 VTSPSV---PRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFI 454
            TSPS     R  G  ET  S  S WKKGK LG GTFG VY+GFN               
Sbjct: 206 -TSPSAVHGSRIGGGYETSPSGFSTWKKGKFLGSGTFGQVYLGFN--------------- 265

Query: 455 MVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKL 514
                E G+MCA+KEV + SDD  SKE  KQL QEI LL++L HPNIVQYYGSE   + L
Sbjct: 266 ----SEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETL 325

Query: 515 YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISK 574
            +YLEYVSGGSI+KLL++YG F E  I++YT+QIL+GLAYLH + TVHR          K
Sbjct: 326 SVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHR--------DIK 385

Query: 575 EQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHM 634
               L+     +     G     +A HV        T +     F  S            
Sbjct: 386 GANILVDPNGEIKLADFG-----MAKHV--------TAFSTMLSFKGS------------ 445

Query: 635 PDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIP 694
           P     +V+ + NG   AVDIWSLGCT+LEMAT+KPPWSQ+EGVAA+FKIGNSK+ P IP
Sbjct: 446 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIP 505

Query: 695 EHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNGV 754
           +HLS+D K+F+RLCLQRNP  RPTA+QLL+HPF++    V     +S P D  P   +G 
Sbjct: 506 DHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVAS---TSLPKDFPPRSYDGN 557

Query: 755 KILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSPQ 813
             L   +     L  D+        +S+ +   S  ++    S PVSP  SP L    P 
Sbjct: 566 FSLPTREPYPGRLSHDN---YAKQPLSRTIKSPSRENVRAITSLPVSPCSSP-LRQLGPA 557

BLAST of Spg002974.1 vs. TAIR 10
Match: AT1G53570.3 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 288.1 bits (736), Expect = 2.6e-77
Identity = 218/548 (39.78%), Postives = 281/548 (51.28%), Query Frame = 0

Query: 275 HPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEY--SPVPSSRMTSPGP 334
           H D + G +   +S  S  +SG      D        + RG  ++  +  P S       
Sbjct: 86  HQDHVSGSTSGSTSVSSVSSSG----SADDQSQLVASRGRGDVKFNVAAAPRSPERVSPK 145

Query: 335 SSRIQSGAVTPIHPR-AGAPPAESQTCWPDE----KQTHRLPLPPIAISNSSPFSHSNSA 394
           ++ I +   +P H R +G    ES T   D+     + H LP PP     +SP       
Sbjct: 146 AATITTRPTSPRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP-----TSP------- 205

Query: 395 VTSPSV---PRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFI 454
            TSPS     R  G  ET  S  S WKKGK LG GTFG VY+GFN               
Sbjct: 206 -TSPSAVHGSRIGGGYETSPSGFSTWKKGKFLGSGTFGQVYLGFN--------------- 265

Query: 455 MVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKL 514
                E G+MCA+KEV + SDD  SKE  KQL QEI LL++L HPNIVQYYGSE   + L
Sbjct: 266 ----SEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETL 325

Query: 515 YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISK 574
            +YLEYVSGGSI+KLL++YG F E  I++YT+QIL+GLAYLH + TVHR          K
Sbjct: 326 SVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHR--------DIK 385

Query: 575 EQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHM 634
               L+     +     G     +A HV        T +     F  S            
Sbjct: 386 GANILVDPNGEIKLADFG-----MAKHV--------TAFSTMLSFKGS------------ 445

Query: 635 PDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIP 694
           P     +V+ + NG   AVDIWSLGCT+LEMAT+KPPWSQ+EGVAA+FKIGNSK+ P IP
Sbjct: 446 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIP 505

Query: 695 EHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNGV 754
           +HLS+D K+F+RLCLQRNP  RPTA+QLL+HPF++    V     +S P D  P   +G 
Sbjct: 506 DHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVAS---TSLPKDFPPRSYDGN 557

Query: 755 KILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSPQ 813
             L   +     L  D+        +S+ +   S  ++    S PVSP  SP L    P 
Sbjct: 566 FSLPTREPYPGRLSHDN---YAKQPLSRTIKSPSRENVRAITSLPVSPCSSP-LRQLGPA 557

BLAST of Spg002974.1 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 285.4 bits (729), Expect = 1.7e-76
Identity = 218/548 (39.78%), Postives = 280/548 (51.09%), Query Frame = 0

Query: 275 HPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEY--SPVPSSRMTSPGP 334
           H D + G +   +S  S  +SG      D        + RG  ++  +  P S       
Sbjct: 86  HQDHVSGSTSGSTSVSSVSSSG----SADDQSQLVASRGRGDVKFNVAAAPRSPERVSPK 145

Query: 335 SSRIQSGAVTPIHPR-AGAPPAESQTCWPDE----KQTHRLPLPPIAISNSSPFSHSNSA 394
           ++ I +   +P H R +G    ES T   D+     + H LP PP     +SP       
Sbjct: 146 AATITTRPTSPRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP-----TSP------- 205

Query: 395 VTSPSV---PRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFI 454
            TSPS     R  G  ET  S  S WKKGK LG GTFG VY+GFN               
Sbjct: 206 -TSPSAVHGSRIGGGYETSPSGFSTWKKGKFLGSGTFGQVYLGFN--------------- 265

Query: 455 MVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKL 514
                E G+MCA+KEV + SDD  SKE  KQL QEI LL++L HPNIVQYYGSE   + L
Sbjct: 266 ----SEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETL 325

Query: 515 YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISK 574
            +YLEYVSGGSI+KLL++YG F E  I++YT+QIL+GLAYLH + TVHR          K
Sbjct: 326 SVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHR--------DIK 385

Query: 575 EQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHM 634
               L+     +     G     +A HV        T +     F  S            
Sbjct: 386 GANILVDPNGEIKLADFG-----MAKHV--------TAFSTMLSFKGS------------ 445

Query: 635 PDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIP 694
           P     +V+ + NG   AVDIWSLGCT+LEMAT+KPPWSQ+EGVAA+FKIGNSK+ P IP
Sbjct: 446 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIP 505

Query: 695 EHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNGV 754
           +HLS+D K+F+RLCLQRNP  RPTA+QLL+HPF++    V     +S P D  P   +G 
Sbjct: 506 DHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVAS---TSLPKDFPPRSYDGN 558

Query: 755 KILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSPQ 813
             L   +       S    A     +S+ +   S  ++    S PVSP  SP L    P 
Sbjct: 566 FSLQPTREPYPGRLSHDNYA--KQPLSRTIKSPSRENVRAITSLPVSPCSSP-LRQLGPA 558

BLAST of Spg002974.1 vs. TAIR 10
Match: AT1G53570.4 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 282.7 bits (722), Expect = 1.1e-75
Identity = 218/549 (39.71%), Postives = 280/549 (51.00%), Query Frame = 0

Query: 275 HPDVILGGSGHCSSPGSGHNSGHNSMGGDMAGHFFWQQSRGSPEY--SPVPSSRMTSPGP 334
           H D + G +   +S  S  +SG      D        + RG  ++  +  P S       
Sbjct: 86  HQDHVSGSTSGSTSVSSVSSSG----SADDQSQLVASRGRGDVKFNVAAAPRSPERVSPK 145

Query: 335 SSRIQSGAVTPIHPR-AGAPPAESQTCWPDE----KQTHRLPLPPIAISNSSPFSHSNSA 394
           ++ I +   +P H R +G    ES T   D+     + H LP PP     +SP       
Sbjct: 146 AATITTRPTSPRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP-----TSP------- 205

Query: 395 VTSPSV---PRSPGRTETPASPGSRWKKGKLLGRGTFGHVYVGFNRLIVLSDISDYDTFI 454
            TSPS     R  G  ET  S  S WKKGK LG GTFG VY+GFN               
Sbjct: 206 -TSPSAVHGSRIGGGYETSPSGFSTWKKGKFLGSGTFGQVYLGFN--------------- 265

Query: 455 MVLIPESGEMCAMKEVTLFSDDAKSKESAKQLMQEIALLSRLRHPNIVQYYGSETVGDKL 514
                E G+MCA+KEV + SDD  SKE  KQL QEI LL++L HPNIVQYYGSE   + L
Sbjct: 266 ----SEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETL 325

Query: 515 YIYLEYVSGGSIYKLLQEYGQFGELAIRSYTQQILSGLAYLHAKTTVHRIELLTSSGISK 574
            +YLEYVSGGSI+KLL++YG F E  I++YT+QIL+GLAYLH + TVHR          K
Sbjct: 326 SVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYLHGRNTVHR--------DIK 385

Query: 575 EQTYLLTQMVVLNWQTLGWRSISLANHVPCRLREALTGWHLRSEFIASVSESDKYMMIHM 634
               L+     +     G     +A HV        T +     F  S            
Sbjct: 386 GANILVDPNGEIKLADFG-----MAKHV--------TAFSTMLSFKGS------------ 445

Query: 635 PDPSLSQVIKNSNGCNLAVDIWSLGCTVLEMATTKPPWSQYEGVAAMFKIGNSKELPVIP 694
           P     +V+ + NG   AVDIWSLGCT+LEMAT+KPPWSQ+EGVAA+FKIGNSK+ P IP
Sbjct: 446 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIP 505

Query: 695 EHLSDDGKDFVRLCLQRNPHHRPTAAQLLDHPFVKYAAPVERPFLSSEPSDATPGVTNG- 754
           +HLS+D K+F+RLCLQRNP  RPTA+QLL+HPF++    V     +S P D  P   +G 
Sbjct: 506 DHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVAS---TSLPKDFPPRSYDGN 557

Query: 755 VKILGIGQSRTTSLDSDSRLAIHSSRVSKAVLHASEISIPRNISCPVSPIGSPLLHSRSP 813
             +    +     L  D+      SR  K    +   ++    S PVSP  SP L    P
Sbjct: 566 FSLQPTREPYPGRLSHDNYAKQPLSRTIK----SPRENVRAITSLPVSPCSSP-LRQLGP 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013246.10.0e+0085.70Mitogen-activated protein kinase kinase kinase YODA, partial [Cucurbita argyrosp... [more]
KAG6574189.10.0e+0085.59Mitogen-activated protein kinase kinase kinase YODA, partial [Cucurbita argyrosp... [more]
XP_022968042.10.0e+0085.38mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucurbita m... [more]
XP_038884021.10.0e+0084.62mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Benincasa h... [more]
XP_038884051.10.0e+0084.62mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [Benincasa h... [more]
Match NameE-valueIdentityDescription
Q9CAD59.0e-22952.61Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
F4HRJ42.3e-7539.78Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q9C5H58.3e-6537.95Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702... [more]
Q405412.4e-4835.59Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220403.0e-4633.51Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1HW310.0e+0085.38mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucurbita... [more]
A0A6J1FZV10.0e+0085.28mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucurbita... [more]
A0A6J1CGN40.0e+0084.96mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia ... [more]
A0A0A0L9U60.0e+0083.88Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182... [more]
A0A5D3DHE50.0e+0083.77Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma... [more]
Match NameE-valueIdentityDescription
AT1G63700.16.4e-23052.61Protein kinase superfamily protein [more]
AT1G53570.22.6e-7739.78mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.32.6e-7739.78mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.11.7e-7639.78mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.41.1e-7539.71mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 579..725
e-value: 5.4E-21
score: 76.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 408..553
e-value: 1.2E-29
score: 105.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 286..412
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 313..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 797..830
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 932..949
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 793..830
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 371..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 930..949
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..192
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..192
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 1..553
coord: 631..871
NoneNo IPR availablePANTHERPTHR48016:SF13MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE YODAcoord: 1..553
coord: 631..871
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 450..553
e-value: 2.3E-20
score: 73.1
coord: 632..718
e-value: 4.0E-14
score: 52.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 410..718
score: 33.502541
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 405..720

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spg002974Spg002974gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg002974.1-five_prime_utrSpg002974.1-five_prime_utr-scaffold6:1424354..1424615five_prime_UTR
Spg002974.1-five_prime_utrSpg002974.1-five_prime_utr-scaffold6:1424715..1424911five_prime_UTR
Spg002974.1-five_prime_utrSpg002974.1-five_prime_utr-scaffold6:1425678..1425940five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1425941..1426497CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1426708..1427439CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1427526..1427582CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1427667..1427754CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1427857..1427919CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1428560..1428720CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1428818..1428916CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1429580..1429823CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1430192..1430431CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1430826..1430917CDS
Spg002974.1-cdsSpg002974.1-cds-scaffold6:1431019..1431535CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spg002974.1-three_prime_utrSpg002974.1-three_prime_utr-scaffold6:1431536..1432099three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spg002974.1Spg002974.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity